阅读说明：本技术 一种菌酶协同发酵艾草饲料及其制备方法和应用 (Wormwood feed fermented by cooperation of bacterial enzymes and preparation method and application thereof ) 是由 马瑞雪 朱平华 张建明 朱崇淼 于 2021-09-30 设计创作，主要内容包括：本发明中草药资源生物饲料化利用技术领域,具体公开了一种菌酶协同发酵艾草饲料及其制备方法和应用,发酵基料以全株艾草粉为主料,玉米粉为辅料,接种混合菌种发酵培养液并添加纤维素酶进行发酵酶解,利用发酵工艺和酶技术协同作用制得菌酶协同发酵艾草饲料。其中,发酵菌种包括植物乳杆菌、粪肠球菌和产朊假丝酵母。本发明的菌酶协同发酵艾草饲料纤维物质含量显著降低,蛋白质含量升高,生物活性物质挥发油、总黄酮、鞣质等含量明显提高,气味和适口性显著改善,应用于蛋鸡养殖,可提高生产性能,增强机体免疫力,改善蛋产品品质,提高养殖经济效益。(The invention belongs to the technical field of biological feed utilization of Chinese herbal medicine resources, and particularly discloses a bacterium-enzyme synergistic fermented wormwood feed and a preparation method and application thereof. Wherein the fermentation strain comprises Lactobacillus plantarum, enterococcus faecalis and Candida utilis. The mugwort feed fermented by the bacterial enzymes in a synergistic manner has the advantages of obviously reducing the content of fiber substances, increasing the content of protein, obviously improving the contents of volatile oil, total flavone, tannin and the like of bioactive substances, obviously improving the smell and palatability, being applied to the breeding of laying hens, improving the production performance, enhancing the immunity of the organism, improving the quality of egg products and improving the economic benefit of the breeding.)

1. A preparation method of a mugwort feed by bacterium-enzyme synergistic fermentation is characterized by comprising the following steps:

(1) preparing a fermentation base material: uniformly mixing wormwood powder and corn powder, and sterilizing with high-pressure steam to obtain a fermentation base material;

(2) inoculating and fermenting: and (2) uniformly mixing the fermentation base material prepared in the step (1) with the mixed strain fermentation culture solution and cellulase, adjusting the water content to obtain a fermentation mixed material, then fermenting, and drying after fermentation to obtain the mugwort feed with the bacterium-enzyme synergistic fermentation.

2. The method according to claim 1, wherein the wormwood powder in the step (1) is prepared by crushing whole wormwood, and sieving the crushed wormwood with a 60-mesh sieve.

3. The preparation method according to claim 1, wherein the fermentation base material in the step (1) comprises 90 parts of wormwood powder and 10 parts of corn powder by weight.

4. The method according to claim 1, wherein the mixed fermentation broth in step (2) comprises lactobacillus plantarum, enterococcus faecalis, and candida utilis.

5. The method according to claim 4, wherein the mixed culture fermentation broth is prepared by:

(1) inoculating Lactobacillus plantarum in liquid culture medium to OD₆₀₀Obtaining Lactobacillus plantarum fermentation broth with the value of 2.5, inoculating enterococcus faecalis in liquid medium to OD₆₀₀Obtaining enterococcus faecalis fermentation liquor with the value of 2.2, inoculating candida utilis in a liquid culture medium to OD₆₀₀Obtaining candida utilis fermentation liquor with the value of 1.8;

(2) and (2) mixing the lactobacillus plantarum fermentation liquor, the enterococcus faecalis fermentation liquor and the candida utilis fermentation liquor prepared in the step (1) according to the volume ratio of 2:2:1 to obtain a mixed fermentation culture solution.

6. The method according to claim 1, wherein the cellulase in the step (2) has an enzymatic activity of 5X 10⁴U/g。

7. The preparation method of claim 1, wherein the fermentation base material, the mixed strain fermentation culture solution and the cellulase are uniformly mixed according to the weight ratio of 100 to (1-3) to (0.2-1) in the step (2) to obtain the fermentation mixed material.

8. The method according to claim 1, wherein the water content in step (2) is 40%, the fermentation is performed at 35-37 ℃ for 4 days, and the drying is performed at 55 ℃ until the water content is 10%.

9. The mugwort feed synergistically fermented with the bacterial enzyme prepared by the production method according to any one of claims 1 to 8.

10. Use of the bacteriase-co-fermented wormwood feed according to claim 9 in poultry farming.

Technical Field

The invention relates to the technical field of biological feed utilization of Chinese herbal medicine resources, in particular to a mugwort feed fermented by the cooperation of bacterial enzymes and a preparation method and application thereof.

Background

The outstanding problems of the animal husbandry in China all the year round are still the serious shortage of feed resources, particularly high-quality feed raw materials, and meanwhile, the safety of livestock and poultry products and the pollution to the breeding environment are also important problems to be solved urgently. In China, commercial feed containing growth-promoting drug feed additives (except traditional Chinese medicines) is prohibited from being produced comprehensively from 7 months to 1 day in 2020, enterprises and research and development institutions are encouraged to accelerate the development of biological feed antibiotic substitute products, the antibiotic substitution in the whole industry is promoted, and the quality safety of livestock and poultry products is ensured.

The biological feed comprises fermented feed, enzymolysis feed, bacterial-enzyme synergistic fermented feed, biological feed additive and the like, wherein the bacterial-enzyme synergistic fermented feed is prepared from feed raw materials, enzyme preparations and microorganisms which are allowed to be used by national relevant regulations such as feed raw material catalog and feed additive variety catalog (2013) of Ministry of agricultural and rural areas, and is produced by the synergistic effect of fermentation engineering and enzyme engineering technologies, and single feed and mixed feed containing the microorganisms or metabolites thereof. Biological feeds are gradually accepted by the whole industry as a new safe and green alternative feed with the most potential for solving or relieving the problems of feed resource shortage, livestock and poultry product safety and breeding environment pollution. The bacterial enzyme synergistic fermented feed is widely applied to livestock breeding by utilizing the synergistic effect of microbial fermentation and enzyme preparation enzymolysis of 1+1 > 2 in the production process.

Artemisia argyi L.et Vant.L.of Compositae, which is an important medicinal material used in clinic in China, contains abundant carbohydrates, proteins, essential amino acids, minerals and vitamins, and is rich in volatile oils, flavonoids, tannins, phenylpropanoids, terpenoids and other bioactive substances, and has pharmacological health-care functions of bacteriostasis, antivirus, antioxidation, anticancer, anti-inflammation and the like. The Chinese wormwood resource yield is high, the planting distribution is wide, and according to the published data of the Chinese industry information network, the Chinese wormwood yield is 19.2 ten thousand tons in 2020, the dry wormwood yield is 11.5 ten thousand tons, and the Chinese wormwood is almost spread in China except extremely arid and alpine regions. However, many studies report that the effect of directly applying wormwood to animal feed is poor, and the reason may be that the livestock and poultry cannot effectively absorb the nutritional ingredients and bioactive substances, so that the nutritional and health-care values of wormwood cannot be fully utilized.

The mugwort resource is developed into high-value special biological feed by utilizing the bacterium-enzyme synergistic fermentation technology, the mugwort feed value can be improved, the mugwort bioactivity can be enhanced, the mugwort synergistic fermentation technology is applied to animal production, the animal production performance can be improved, the intestinal microecology can be improved, the immunity of an animal body can be enhanced, and the mugwort synergistic fermentation technology has important significance for widely opening the source of feed raw materials, improving the utilization level of the raw materials, relieving the lack of feed resources, ensuring the quality safety of livestock and poultry products and replacing antibiotics.

Disclosure of Invention

The invention provides a mugwort feed fermented by synergy of bacteria and enzymes, and a preparation method and application thereof.

In order to achieve the purpose, the invention provides the following scheme:

a preparation method of a mugwort feed by bacterium-enzyme synergistic fermentation comprises the following steps:

(1) preparing a fermentation base material: uniformly mixing wormwood powder and corn powder, and sterilizing with high-pressure steam to obtain a fermentation base material;

(2) inoculating and fermenting: and (2) uniformly mixing the fermentation base material prepared in the step (1) with the mixed fermentation culture solution and cellulase, adjusting the water content to obtain a fermentation mixed material, then fermenting, and drying after fermentation to obtain the mugwort feed with the cooperation of the bacterial enzyme and the fermentation enzyme.

As a further optimization of the invention, the preparation method of the wormwood powder in the step (1) is that the whole wormwood is crushed and sieved by a 60-mesh sieve.

As a further optimization of the invention, the fermentation base material in the step (1) comprises 90 parts of wormwood powder and 10 parts of corn powder in parts by weight.

As a further optimization of the invention, the mixed fermentation culture solution in the step (2) comprises lactobacillus plantarum, enterococcus faecalis and candida utilis, and the preparation method comprises the following steps:

inoculating Lactobacillus plantarum in liquid culture medium to OD₆₀₀Obtaining Lactobacillus plantarum fermentation broth with the value of 2.5, inoculating enterococcus faecalis in liquid medium to OD₆₀₀Obtaining enterococcus faecalis fermentation liquor with the value of 2.2, inoculating candida utilis in a liquid culture medium to OD₆₀₀Obtaining candida utilis fermentation liquor with the value of 1.8; mixing the prepared lactobacillus plantarum fermentation liquor, enterococcus faecalis fermentation liquor and candida utilis fermentation liquor according to the volume ratio of 2:2:1 to obtain mixed fermentation culture solution.

As a further optimization of the invention, the enzyme activity of the cellulase in the step (2) is 5 x 10⁴U/g。

As a further optimization of the invention, in the step (2), the fermentation base material, the fermentation bacteria agent and the cellulase are uniformly mixed according to the weight ratio of 100: 1-3: 0.2-1 to obtain the fermentation mixed material.

As a further optimization of the invention, in the step (2), the fermentation base material, the mixed fermentation culture solution and the cellulase are uniformly mixed according to the weight ratio of 100: 2:1 to obtain the fermentation mixed material.

As a further optimization of the invention, in the step (2), the water content is 40%, the fermentation is carried out at the temperature of 35-37 ℃ for 4d, and the drying is carried out at the low temperature of 55 ℃ until the water content is 10%.

The invention also provides the mugwort feed synergistically fermented by the bacterial enzyme prepared by the preparation method.

The invention also provides application of the bacterium-enzyme synergistic fermented wormwood feed in poultry breeding.

The invention discloses the following technical effects:

the invention takes the whole plant wormwood powder as the main material and the corn flour as the auxiliary material, inoculates the mixed strain fermentation culture solution and adds the cellulase for fermentation enzymolysis, and prepares the mugwort feed by utilizing the synergistic effect of the fermentation process and the enzyme technology, thereby improving the feeding value of the mugwort, realizing the biological feed utilization of Chinese herbal medicine resources, having positive effects on relieving the current situation of feed resource shortage, ensuring the safety of livestock and poultry products, solving the problem of feed 'resistance' and relieving the environmental pollution of livestock and poultry breeding, in particular:

(1) according to the invention, aiming at the nutritional characteristics and pharmacological activity of the wormwood, scientific compatibility and combination of functional probiotics are optimized, and meanwhile, cellulase is added for synergistic fermentation of the wormwood, so that the fiber content of a fermentation product is obviously reduced compared with that before fermentation, the crude protein content is increased, the contents of volatile oil, total flavone and tannin are obviously increased, the nutritional level is obviously increased, meanwhile, the odor property and palatability of the wormwood are also obviously improved, animal feeding and digestive absorption can be promoted after the wormwood is fermented by the cooperation of the bacterial enzymes, and the utilization rate is increased.

(2) The biological wormwood feed rich in bioactive substances and beneficial metabolites and prepared by fermenting wormwood with bacterial enzymes in a synergistic manner is applied to poultry cultivation, can meet partial nutritional requirements of animals, can fully exert the pharmaceutical effect and the health-care effect of the animals, can adjust the intestinal flora of the animals, enhances the oxidation resistance and the immune function of organisms, improves the production performance of the animals, reduces the production cost and improves the economic benefit of cultivation. Particularly, the compound premix is applied to laying hen production, and can improve egg laying performance, egg quality and antioxidant capacity of laying hens.

Detailed Description

Reference will now be made in detail to various exemplary embodiments of the invention, the detailed description should not be construed as limiting the invention but as a more detailed description of certain aspects, features and embodiments of the invention.

It is to be understood that the terminology used herein is for the purpose of describing particular embodiments only and is not intended to be limiting of the invention. Further, for numerical ranges in this disclosure, it is understood that each intervening value, between the upper and lower limit of that range, is also specifically disclosed. Every smaller range between any stated value or intervening value in a stated range and any other stated or intervening value in a stated range is encompassed within the invention. The upper and lower limits of these smaller ranges may independently be included or excluded in the range.

Unless defined otherwise, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this invention belongs. Although only preferred methods and materials are described herein, any methods and materials similar or equivalent to those described herein can be used in the practice or testing of the present invention. All documents mentioned in this specification are incorporated by reference herein for the purpose of disclosing and describing the methods and/or materials associated with the documents. In case of conflict with any incorporated document, the present specification will control.

It will be apparent to those skilled in the art that various modifications and variations can be made in the specific embodiments of the present disclosure without departing from the scope or spirit of the disclosure. Other embodiments will be apparent to those skilled in the art from consideration of the specification. The description and examples are intended to be illustrative only.

As used herein, the terms "comprising," "including," "having," "containing," and the like are open-ended terms that mean including, but not limited to.

Example 1

(1) Preparation of fermentation base material: selecting whole wormwood, crushing to 60 meshes to obtain wormwood powder, uniformly mixing 90 parts of wormwood powder and 10 parts of corn in parts by weight, and performing high-pressure steam sterilization to obtain a fermentation base material.

(2) Production of mixed fermentation culture solution: respectively culturing lactobacillus plantarum, enterococcus faecalis and candida utilis to OD by liquid state fermentation₆₀₀After the values reach 2.5, 2.2 and 1.8, mixing the 3 single-strain culture solutions according to the volume ratio of 2:2:1 to obtain a mixed-strain fermentation culture solution.

(3) Inoculating and fermenting the base material: fermenting the base material, mixed fermentation culture solution and enzyme activity of 5 × 10⁴Uniformly mixing U/g cellulase according to the weight ratio of 100: 2:1 to obtain a fermentation mixed material, adjusting the water content of the fermentation mixed material to 40%, controlling the fermentation at the temperature of 35-37 ℃ for 4 days, and drying the fermentation product at the low temperature of 55 ℃ after the fermentation is finished until the water content is 10% to obtain the bacterium-enzyme synergistic fermentation wormwood feed.

Example 2

(1) Preparation of fermentation base material: selecting whole wormwood, crushing to 60 meshes to obtain wormwood powder, uniformly mixing 90 parts of wormwood powder and 10 parts of corn in parts by weight, and performing high-pressure steam sterilization to obtain a fermentation base material.

(2) Production of mixed fermentation culture solution: respectively culturing lactobacillus plantarum, enterococcus faecalis and candida utilis to OD by liquid state fermentation₆₀₀After the values reach 2.5, 2.2 and 1.8, mixing the 3 single-strain culture solutions according to the volume ratio of 2:2:1 to obtain a mixed-strain fermentation culture solution.

(3) Inoculating and fermenting the base material: fermenting the base material, mixed fermentation culture solution and enzyme activity of 5 × 10⁴Uniformly mixing U/g cellulase according to the weight ratio of 100: 1: 0.2 to obtain a fermentation mixed material, adjusting the water content of the fermentation mixed material to 40%, controlling the fermentation at the temperature of 35-37 ℃ for 3 days, and drying the fermentation product at the low temperature of 55 ℃ after the fermentation is finished until the water content is 10% to obtain the bacterium-enzyme synergistic fermentation wormwood feed.

Example 3

(1) Preparation of fermentation base material: selecting whole wormwood, crushing to 60 meshes to obtain wormwood powder, uniformly mixing 90 parts of wormwood powder and 10 parts of corn in parts by weight, and performing high-pressure steam sterilization to obtain a fermentation base material.

(2) Production of mixed fermentation culture solution: respectively culturing lactobacillus plantarum, enterococcus faecalis and candida utilis to OD by liquid state fermentation₆₀₀After the values reach 2.5, 2.2 and 1.8, mixing the 3 single-strain culture solutions according to the volume ratio of 2:2:1 to obtain a mixed-strain fermentation culture solution.

(3) Inoculating and fermenting the base material: fermenting the base material, mixed fermentation culture solution and enzyme activity of 5 × 10⁴Uniformly mixing U/g cellulase according to the weight ratio of 100: 1: 0.5 to obtain a fermentation mixed material, adjusting the water content of the fermentation mixed material to 45%, controlling the fermentation at the temperature of 35-37 ℃ for 4 days, and drying the fermentation product at the low temperature of 55 ℃ after the fermentation is finished until the water content is 10% to obtain the bacterium-enzyme synergistic fermentation wormwood feed.

Example 4

(1) Preparation of fermentation base material: selecting whole wormwood, crushing to 60 meshes to obtain wormwood powder, uniformly mixing 90 parts of wormwood powder and 10 parts of corn in parts by weight, and performing high-pressure steam sterilization to obtain a fermentation base material.

(2) Production of mixed fermentation culture solution: respectively culturing lactobacillus plantarum, enterococcus faecalis and candida utilis to OD by liquid state fermentation₆₀₀The values are 2.5 and 2.2And 1.8, mixing the 3 single-strain culture solutions according to the volume ratio of 2:2:1 to obtain a mixed-strain fermentation culture solution.

(3) Inoculating and fermenting the base material: fermenting the base material, mixed fermentation culture solution and enzyme activity of 5 × 10⁴Uniformly mixing U/g cellulase according to the weight ratio of 100: 1 to obtain a fermentation mixed material, adjusting the water content of the fermentation mixed material to be 50%, controlling the fermentation at the temperature of 35-37 ℃ for 5 days, and drying the fermentation product at the low temperature of 55 ℃ after the fermentation is finished until the water content is 10% to obtain the bacterium-enzyme synergistic fermentation wormwood feed.

Example 5

(1) Preparation of fermentation base material: selecting whole wormwood, crushing to 60 meshes to obtain wormwood powder, uniformly mixing 90 parts of wormwood powder and 10 parts of corn in parts by weight, and performing high-pressure steam sterilization to obtain a fermentation base material.

(2) Production of mixed fermentation culture solution: respectively culturing lactobacillus plantarum, enterococcus faecalis and candida utilis to OD by liquid state fermentation₆₀₀After the values reach 2.5, 2.2 and 1.8, mixing the 3 single-strain culture solutions according to the volume ratio of 2:2:1 to obtain a mixed-strain fermentation culture solution.

(3) Inoculating and fermenting the base material: fermenting the base material, mixed fermentation culture solution and enzyme activity of 5 × 10⁴Uniformly mixing U/g cellulase according to the weight ratio of 100: 2: 0.2 to obtain a fermentation mixed material, adjusting the water content of the fermentation mixed material to 45%, controlling the fermentation at 35-37 ℃ for 5 days, and drying the fermentation product at 55 ℃ to 10% of water content after the fermentation is finished to obtain the bacterium-enzyme synergistic fermentation wormwood feed.

Example 6

(1) Preparation of fermentation base material: selecting whole wormwood, crushing to 60 meshes to obtain wormwood powder, uniformly mixing 90 parts of wormwood powder and 10 parts of corn in parts by weight, and performing high-pressure steam sterilization to obtain a fermentation base material.

(2) Production of mixed fermentation culture solution: respectively culturing lactobacillus plantarum, enterococcus faecalis and candida utilis to OD by liquid state fermentation₆₀₀After the values reach 2.5, 2.2 and 1.8, mixing the 3 single-strain culture solutions according to the volume ratio of 2:2:1 to obtain a mixed-strain fermentation culture solution.

(3) Inoculating and fermenting the base material: fermenting the base material, mixed fermentation culture solution and enzyme activity of 5 × 10⁴U/g cellulase by weightUniformly mixing the materials according to the ratio of 100: 2: 0.5 to obtain a fermentation mixed material, adjusting the water content of the fermentation mixed material to be 50%, controlling the temperature to be 35-37 ℃ for fermentation for 3d, and drying the fermentation product at the low temperature of 55 ℃ after the fermentation is finished until the water content is 10% to obtain the bacterium-enzyme synergistic fermentation wormwood feed.

Example 7

(1) Preparation of fermentation base material: selecting whole wormwood, crushing to 60 meshes to obtain wormwood powder, uniformly mixing 90 parts of wormwood powder and 10 parts of corn in parts by weight, and performing high-pressure steam sterilization to obtain a fermentation base material.

(2) Production of mixed fermentation culture solution: respectively culturing lactobacillus plantarum, enterococcus faecalis and candida utilis to OD by liquid state fermentation₆₀₀After the values reach 2.5, 2.2 and 1.8, mixing the 3 single-strain culture solutions according to the volume ratio of 2:2:1 to obtain a mixed-strain fermentation culture solution.

(3) Inoculating and fermenting the base material: fermenting the base material, mixed fermentation culture solution and enzyme activity of 5 × 10⁴Uniformly mixing U/g cellulase according to the weight ratio of 100: 3: 0.2 to obtain a fermentation mixed material, adjusting the water content of the fermentation mixed material to be 50%, controlling the fermentation at the temperature of 35-37 ℃ for 4 days, and drying the fermentation product at the low temperature of 55 ℃ after the fermentation is finished until the water content is 10% to obtain the bacterium-enzyme synergistic fermentation wormwood feed.

Example 8

(1) Preparation of fermentation base material: selecting whole wormwood, crushing to 60 meshes to obtain wormwood powder, uniformly mixing 90 parts of wormwood powder and 10 parts of corn in parts by weight, and performing high-pressure steam sterilization to obtain a fermentation base material.

(2) Production of mixed fermentation culture solution: respectively culturing lactobacillus plantarum, enterococcus faecalis and candida utilis to OD by liquid state fermentation₆₀₀After the values reach 2.5, 2.2 and 1.8, mixing the 3 single-strain culture solutions according to the volume ratio of 2:2:1 to obtain a mixed-strain fermentation culture solution.

(3) Inoculating and fermenting the base material: fermenting the base material, mixed fermentation culture solution and enzyme activity of 5 × 10⁴Uniformly mixing U/g cellulase according to the weight ratio of 100: 3: 0.5 to obtain a fermentation mixed material, adjusting the water content of the fermentation mixed material to 40%, controlling the fermentation at the temperature of 35-37 ℃ for 5 days, and drying the fermentation product at the low temperature of 55 ℃ after the fermentation is finished until the water content is 10% to obtain the bacterium-enzyme synergistic fermentation wormwood feed.

Example 9

(1) Preparation of fermentation base material: selecting whole wormwood, crushing to 60 meshes to obtain wormwood powder, uniformly mixing 90 parts of wormwood powder and 10 parts of corn in parts by weight, and performing high-pressure steam sterilization to obtain a fermentation base material.

(2) Production of mixed fermentation culture solution: respectively culturing lactobacillus plantarum, enterococcus faecalis and candida utilis to OD by liquid state fermentation₆₀₀After the values reach 2.5, 2.2 and 1.8, mixing the 3 single-strain culture solutions according to the volume ratio of 2:2:1 to obtain a mixed-strain fermentation culture solution.

(3) Inoculating and fermenting the base material: fermenting the base material, mixed fermentation culture solution and enzyme activity of 5 × 10⁴Uniformly mixing U/g cellulase according to the weight ratio of 100: 3: 1 to obtain a fermentation mixed material, adjusting the water content of the fermentation mixed material to 45%, controlling the fermentation at the temperature of 35-37 ℃ for 3 days, and drying the fermentation product at the low temperature of 55 ℃ after the fermentation is finished until the water content is 10% to obtain the bacterium-enzyme synergistic fermentation wormwood feed.

Example 10

The pH, viable count, Neutral Detergent Fiber (NDF), Acid Detergent Fiber (ADF), Crude Protein (CP), volatile oil, total flavonoids, and tannin contents of the fungus-enzyme co-fermented wormwood feeds prepared in examples 1 to 9 were measured, and the results are shown in table 1.

TABLE 1

Carrying out multi-index comprehensive evaluation on detection indexes of the bacterial enzyme synergistic fermented wormwood feed prepared in experimental examples 1-9 in the experimental example 1 by utilizing an approach ideal solution sorting method (TOPSIS), respectively giving weights of-1, -1, 1 and 1 to the pH value, the viable count, Neutral Detergent Fiber (NDF), Acid Detergent Fiber (ADF), Crude Protein (CP), volatile oil, total flavone and tannin content according to the influence degree of each index on the fermentation enzymolysis quality, carrying out weighted summation, and obtaining the maximum comprehensive score of the experimental example 1 as shown in the table 2, wherein the maximum comprehensive score of the experimental example 1 is 0.874, which indicates that under the condition, the comprehensive level of 8 evaluation indexes is the maximum, namely the optimal fermentation enzymolysis process parameters are as follows: the mixing weight ratio of the fermentation base material, the mixed bacteria fermentation culture solution and the cellulose is 100: 2:1, the water content of the fermentation mixed material is 40%, and the fermentation time is 4 d.

TABLE 2

Example 11

The pH value, the number of viable bacteria, Neutral Detergent Fiber (NDF), Acid Detergent Fiber (ADF), Crude Protein (CP), volatile oil, total flavonoids and tannin contents of the fermentation substrate in example 1 were measured, and compared with the fermented mugwort feed prepared in example 1 using the fungal enzyme in cooperation, and the results are shown in Table 3.

TABLE 3

As can be seen from Table 3, the fungus enzymes produced in example 1 synergistically ferment the wormwood feed, and the nutrition composition of the fermentation base material is obviously improved through fermentation and enzymolysis of the lactobacillus plantarum, the enterococcus faecalis and the candida utilis in cooperation with the cellulase, the pH value and the fiber substance content are obviously reduced, and the crude protein content is increased. Meanwhile, compared with the base material before fermentation, the essential oil content, the total flavone content and the tannin content of the mugwort feed synergistically fermented by the bacterial enzyme prepared in the example 1 are obviously improved.

Example 12

Application of bacterium-enzyme synergistic fermented wormwood feed in laying hen breeding

(1) The test method comprises the following steps: 192 healthy and 41-week-old Hailan brown laying hens with similar egg laying performance are selected and randomly divided into a control group and a test group, each group has 6 repetitions, and each repetition has 16 chickens. Feeding conventional basal diet for control group; the experimental group was fed with experimental diets formulated with the bacterial-enzyme synergistic fermented wormwood feed of example 1 and conventional basal diets in a weight ratio of 1: 9. The test period is 49 days, including 7 days of pre-feeding period and 42 days of positive feeding period, three-layer stepped cage culture is adopted, feeding is carried out twice every day, feeding and drinking water are freely carried out, and the constant illumination is 16 hours in the egg laying period.

(2) The average egg weight, laying rate, average daily feed intake and feed-egg ratio of the laying hens of the control group and the experimental group are detected, and the results are shown in table 4.

TABLE 4

The egg shell strength, the egg shell thickness, the yolk color, the egg white height and the hough unit of the laying hens of the control group and the experimental group are detected, and the result is shown in a table 5.

TABLE 5

The serum total oxidation resistance (T-AOC), malondialdehyde content (MDA), total superoxide dismutase (T-SOD), and glutathione peroxidase (GSH-Px) of the laying hens of the control group and the experimental group were measured, and the results are shown in Table 6.

TABLE 6

As can be seen from tables 4-6, compared with the control group, the experimental group added with the bacterial enzyme synergistic fermentation wormwood feed can significantly improve the laying rate of the laying hens and significantly reduce the feed-egg ratio (P <0.05), can significantly improve the protein height and the Hafu unit (P <0.05) of eggs, and simultaneously can significantly improve the glutathione peroxidase activity (GSH-Px) of the serum of the laying hens and reduce the malonaldehyde content (MDA) (P <0.05), and has no significant influence (P > 0.05) on other production performance indexes, egg quality indexes and serum oxidation resistance indexes. The results show that the bacterium-enzyme synergistic fermented wormwood feed can improve the production performance of laying hens, improve the egg quality and improve the oxidation resistance of the laying hens when applied to laying hen breeding.

The above-described embodiments are merely illustrative of the preferred embodiments of the present invention, and do not limit the scope of the present invention, and various modifications and improvements of the technical solutions of the present invention can be made by those skilled in the art without departing from the spirit of the present invention, and the technical solutions of the present invention are within the scope of the present invention defined by the claims.