阅读说明：本技术 一种养颜抗衰老配方奶粉 (Beautifying and anti-aging formula milk powder ) 是由 李双喜 于 2021-06-05 设计创作，主要内容包括：本发明涉及一种养颜抗衰老配方奶粉,以乳固体组合物、透明质酸组合物、弹性蛋白组合物、胶原蛋白组合物、维生素E、维生素C组合物为主要原料,通过优化组合及配比的调整,在几种原料优异的协同作用下,制备的配方奶粉能够养颜抗衰老,与对比实例制备的配方奶粉比较,发现本发明制备的配方奶粉感官指标正常,实验证实肤中羟脯氨酸升高,超氧化物歧化酶(SOD)升高,丙二醛(MDA)降低,同时,皮肤更红润、光滑、充满弹性,表皮更紧致,皱纹减少。因此,本发明配方奶粉具有更好的养颜抗衰老效果。(The invention relates to a beautifying and anti-aging formula milk powder, which takes a milk solid composition, a hyaluronic acid composition, an elastin composition, a collagen composition, a vitamin E composition and a vitamin C composition as main raw materials, and can be used for beautifying and resisting aging under the excellent synergistic effect of the raw materials through optimized combination and adjustment of proportion. Therefore, the formula milk powder has better beautifying and anti-aging effects.)

1. The formula milk powder for beautifying and resisting aging is prepared from the following components in parts by weight: 70000 parts of milk solid composition 99990 parts of hyaluronic acid composition, 0.1-50 parts of vitamin E10-90 parts of vitamin C composition or 30-200 parts of vitamin C composition.

2. The formula milk powder for beautifying and resisting aging is prepared from the following components in parts by weight: 70000 parts of milk solid composition 99990 parts, 0.1-50 parts of hyaluronic acid composition and 0.1-30000 parts of collagen composition.

3. The formula milk powder for beautifying and resisting aging is prepared from the following components in parts by weight: 70000 parts of milk solid composition 99990 parts, 0.1-50 parts of hyaluronic acid composition, 0.1-30000 parts of collagen composition, 10-90 parts of vitamin E or 30-200 parts of vitamin C composition.

4. The formula milk powder for beautifying and resisting aging is prepared from the following components in parts by weight: 70000 parts of milk solid composition 99990 parts, 0.1-50 parts of hyaluronic acid composition, 0.1-30000 parts of elastin composition, 10-90 parts of vitamin E or 30-200 parts of vitamin C composition.

5. The formula milk powder for beautifying and resisting aging is prepared from the following components in parts by weight: 70000 parts of milk solid composition, 99990 parts of hyaluronic acid composition, 0.1-50 parts of elastin composition, 0.1-30000 parts of collagen composition, 10-90 parts of vitamin E and 30-200 parts of vitamin C composition.

6. The formula milk powder for beautifying and resisting aging according to claims 1, 2, 3, 4, and 5, wherein the milk solid composition refers to various dry solids prepared by processing animal milk as a raw material or dry solids prepared by liquid raw material without processing according to a coefficient (if the liquid raw material is liquid, milk solid = (liquid raw material/conversion coefficient), the conversion coefficient can be selected and adjusted according to milk type, source, and the like, preferably 9-15, and most preferably 11.7), and includes, but is not limited to, one or more of whole milk powder, skim milk powder, whey protein powder, desalted whey powder, casein, fresh milk, cream, and milk protein.

7. The formula of milk powder for beautifying face and resisting aging according to claims 1, 2, 3, 4 and 5, wherein the hyaluronic acid composition is one or more of sodium hyaluronate, potassium hyaluronate and calcium hyaluronate.

The elastin composition is a general term for proteins or peptides which are rich in nonpolar amino acids such as glycine, proline, alanine, leucine and valine, usually have a repetitive sequence consisting of a large number of 3-9 amino acids and form a flexible and stable structure, and include, but are not limited to, one or more of bonito elastin peptide, elastin, hydrolyzed elastin (peptide) and elastin-like protein (peptide).

The collagen composition is protein or peptide which is extracted from bones and skins of livestock such as cattle and sheep, fish scales, gelatin, tortoise shells and the like, and is hydrolyzed or not hydrolyzed. Including but not limited to one or more of collagen, fish scale collagen, collagen peptide, and bone collagen.

The vitamin C composition is one or more of L-ascorbic acid, L-calcium ascorbate, magnesium ascorbyl phosphate, L-sodium ascorbate, L-potassium ascorbate, L-ascorbic acid-6-palmitate (ascorbyl palmitate), and acerola cherry.

8. The formula of claims 1, 2, 3, 4, 5, wherein the formula further comprises at least one member selected from the group consisting of conjugated linoleic acid, conjugated linoleic acid glyceride, 2-palmitoleic acid triglyceride 1, 3-dioleate, gamma-linolenic acid, fish oil, soybean oil, peanut oil, sunflower seed oil, rapeseed oil, palm oil, linseed oil, vegetable fat powder, phytosterol esters, phytostanol esters, DHA algal oil, arachidonic acid oil, maltodextrin, low or polyfructose, galacto-oligosaccharide, polydextrose, glucose, corn syrup solids, isomalto-oligosaccharide, inulin, lactoferrin, casein phosphopeptide, chitosan oligosaccharide, soy oligosaccharide, beta-glucan, beta-hydroxy-beta-methylbutyrate, amino acids, soy protein isolate, papaya powder, and mixtures thereof, One or more of pumpkin powder, milk mineral salt, banana powder, blueberry powder, acerola cherry powder, strawberry powder, apple sea-buckthorn, wolfberry fruit, cherry, litchi, black sesame, Chinese yam, pine nut, milk and lotus pistil liquid or processed powder or salt compounds of different raw material sources.

9. The formula of claims 1, 2, 3, 4 and 5, wherein the formula further comprises taurine, lutein, L-carnitine, vitamin A, beta carotene, vitamin D, vitamin K, vitamin B1, vitamin B2, vitamin B6, vitamin B12, niacin, folic acid, pantothenic acid, biotin, choline, selenium agent, calcium agent, iron agent, zinc agent, magnesium agent, copper agent, manganese agent, potassium agent and phosphorus agent. The selenium agent is selected from one or more of sodium selenite, sodium selenate, selenoprotein, selenium-rich edible fungus powder, and L-selenium-methyl selenocysteine; the calcium agent is selected from one or more of calcium carbonate, calcium gluconate, calcium citrate, calcium lactate, L-calcium lactate, calcium hydrogen phosphate, calcium L-threonate, calcium glycinate, calcium aspartate, calcium citrate malate, calcium acetate (calcium acetate), calcium chloride, tricalcium phosphate, calcium vitamin E succinate, calcium glycerophosphate, calcium oxide, calcium sulfate, and bone meal; the iron agent is selected from one or more of ferrous sulfate, ferrous gluconate, ferric ammonium citrate, ferrous fumarate, ferric citrate, ferrous lactate, hemin, ferric pyrophosphate, ferriporphyrin, ferrous glycinate, reduced iron, sodium ferric ethylenediamine tetraacetate, carbonyl iron powder, ferrous carbonate, ferrous citrate, ferrous fumarate, ferrous succinate, heme iron and electrolytic iron; the zinc agent is selected from one or more of zinc sulfate, zinc gluconate, zinc glycinate, zinc oxide, zinc lactate, zinc citrate, zinc chloride, zinc acetate and zinc carbonate; the magnesium agent is selected from one or more of magnesium sulfate, magnesium chloride, magnesium oxide, magnesium carbonate, magnesium hydrogen phosphate and magnesium gluconate; the copper agent is selected from one or more of copper sulfate, copper gluconate, copper citrate and copper carbonate; the manganese agent is selected from one or more of manganese sulfate, manganese chloride, manganese carbonate, manganese citrate and manganese gluconate; the potassium agent is selected from potassium chloride, potassium carbonate, potassium bicarbonate, potassium lactate, potassium citrate, potassium gluconate, potassium dihydrogen phosphate and dipotassium hydrogen phosphate; the potassium agent is one or more selected from tricalcium phosphate and calcium hydrogen phosphate.

Technical Field

The invention belongs to the technical field of food and medicine, and particularly relates to a beautifying and anti-aging formula milk powder.

Background

Aging is a spontaneous inevitable process of living beings over time, and is a complex natural phenomenon which is manifested by structural and functional decline and decline of adaptability and resistance. Due to the pursuit of people for beauty, a medicine or an operation mode is often adopted to resist aging, but the current anti-aging beauty treatment medicines are numerous in western medicines like a double-edged sword, can treat diseases and are easy to generate side effects. To date, the search for a safe and effective natural product formula for maintaining beauty and keeping young is still a hot point of scientific research.

The skin is elastic, mainly because the collagen of the collagen fibers in the dermis layer of the skin forms the scaffold of the skin. As people age, work is busy and tired, the loss of collagen secretion in the skin causes delicate skin to become loose and dry, and problems such as wrinkles and loss of luster occur. Therefore, to maintain youthful skin, the collagen content of the skin must be increased. Collagen is the main component of human tissue, and has inseparable relation with various organ tissues and cells of human body, so that it is used for repairing and regenerating human organ tissues. Elastin is the major cause of sagging, drooping and fine wrinkles of the skin as it ages naturally, elastic fibers break down more and more significantly, most of which are affected by age 70. Many people think that how to supplement collagen is the key point for maintaining youthful skin, and elastin is more important. Elastin acts as a rubber band in the skin, allowing the skin to stretch and fold, and functions like a spring in a mattress to maintain and support the elasticity of the skin. Vitamin C has effects of reducing skin melanin pigmentation, reducing formation of black spot and freckle, and whitening skin. Vitamin E has effects of delaying aging, preventing cancer, preventing multiple chronic diseases, protecting skin from ultraviolet and pollution, and reducing scar and pigment deposition. The milk protein is also an important protein, wherein the whey protein is a protein extracted from milk, has the characteristics of high nutritional value, easy digestion and absorption, various active ingredients and the like, and is one of the accepted high-quality protein supplements for human bodies.

A large amount of data is inquired, and the formula milk powder prepared from the milk solid composition, the hyaluronic acid composition, the elastin composition, the collagen composition, the vitamin E and the vitamin C composition has the effects of beautifying and resisting aging, and has not been reported. Some patents disclose that compositions formulated with collagen, vitamin E, vitamin C, and related components have a cosmetic effect, but do not relate to formula milk, or to formula milk formulated with elastin or hyaluronic acid or milk solids. Due to the formula of the formula milk powder, not only the inherent characteristics of the formula milk powder, such as taste, color, taste and mixing, but also the beautifying and anti-aging effects of the formula are considered.

Through comparison experiments, the formula milk powder prepared by the invention has better effects of beautifying and resisting aging compared with milk powder prepared by collagen and milk solids and milk powder prepared by vitamin E, vitamin C and milk solids.

Disclosure of Invention

Through repeated exploration and animal experiments, the inventor finally finds that the beautifying and anti-aging effects of the beautifying and anti-aging formula milk powder are as follows from strong to weak: the formula milk powder comprises a milk solid composition, a hyaluronic acid composition, an elastin composition, a collagen composition, vitamin E and a vitamin C composition which are used as main raw materials, a formula milk powder which takes the milk solid composition, the hyaluronic acid composition, the elastin composition, the vitamin E and the vitamin C composition as main raw materials, a formula milk powder which takes the milk solid composition, the hyaluronic acid composition, the collagen composition, the vitamin E and the vitamin C composition as main raw materials, a formula milk powder which takes the milk solid composition, the hyaluronic acid composition, the vitamin E or the vitamin C composition as main raw materials, and a formula milk powder which takes the milk solid composition, the hyaluronic acid composition and the collagen composition as main raw materials.

The formula milk powder with several compositions has stronger effects of beautifying and resisting aging than milk powder prepared by collagen and milk solids and milk powder prepared by vitamin E, vitamin C and milk solids on the premise of ensuring unique particles, mouthfeel, color, taste and mixing of the formula milk powder.

The invention realizes excellent synergistic effect mainly by optimizing components and adjusting proportion, ensures the unique color, aroma and taste and brewing of formula milk powder, obviously improves the function of promoting skin cell proliferation, and realizes effective beautifying and anti-aging.

The purpose of the invention is realized by the following modes:

the formula milk powder for beautifying and resisting aging is prepared from the following components in parts by weight: 70000 parts of milk solid composition 99990 parts of hyaluronic acid composition, 0.1-50 parts of vitamin E10-90 parts of vitamin C composition or 30-200 parts of vitamin C composition.

The formula milk powder for beautifying and resisting aging is prepared from the following components in parts by weight: 70000 parts of milk solid composition 99990 parts, 0.1-50 parts of hyaluronic acid composition and 0.1-30000 parts of collagen composition.

The formula milk powder for beautifying and resisting aging is prepared from the following components in parts by weight: 70000 parts of milk solid composition 99990 parts, 0.1-50 parts of hyaluronic acid composition, 0.1-30000 parts of elastin composition, 10-90 parts of vitamin E or 30-200 parts of vitamin C composition.

The formula milk powder for beautifying and resisting aging is prepared from the following components in parts by weight: 70000 parts of milk solid composition 99990 parts, 0.1-50 parts of hyaluronic acid composition, 0.1-30000 parts of collagen composition, 10-90 parts of vitamin E or 30-200 parts of vitamin C composition.

The formula milk powder for beautifying and resisting aging is prepared from the following components in parts by weight: 70000 parts of milk solid composition, 99990 parts of hyaluronic acid composition, 0.1-50 parts of elastin composition, 0.1-30000 parts of collagen composition, 10-90 parts of vitamin E and 30-200 parts of vitamin C composition.

The milk solid composition refers to various dry solids prepared by processing animal milk as a raw material or dry solids prepared by liquid raw materials without processing according to a coefficient (if the liquid raw materials are liquid, milk solid = (liquid raw materials/conversion coefficient), the conversion coefficient can be conventionally selected and adjusted according to the type, source and the like of milk, is preferably 9-15, and is most preferably 11.7), and comprises one or more of whole milk powder, skim milk powder, whey protein powder, desalted whey powder, casein, fresh milk, cream and milk protein.

The hyaluronic acid composition is one or more of sodium hyaluronate, potassium hyaluronate and calcium hyaluronate.

The elastin composition is a general term for proteins or peptides which are rich in nonpolar amino acids such as glycine, proline, alanine, leucine and valine, usually have a repetitive sequence consisting of a large number of 3-9 amino acids and form a flexible and stable structure, and include, but are not limited to, one or more of bonito elastin peptide, elastin, hydrolyzed elastin (peptide) and elastin-like protein (peptide).

The collagen composition is protein extracted from bones and skins of cattle, sheep and other livestock, fish scales, gelatin, tortoise shells and the like, and is hydrolyzed or not hydrolyzed. Including but not limited to one or more of collagen, fish scale collagen, collagen peptide, and bone collagen.

The vitamin C composition is one or more of L-ascorbic acid, L-calcium ascorbate, magnesium ascorbyl phosphate, L-sodium ascorbate, L-potassium ascorbate, L-ascorbic acid-6-palmitate (ascorbyl palmitate), and acerola cherry.

The formula milk powder further comprises conjugated linoleic acid, conjugated linoleic acid glyceride, 1, 3-dioleate 2-palmitic acid triglyceride, gamma-linolenic acid, fish oil, soybean oil, peanut oil, sunflower seed oil, rapeseed oil, palm oil, linseed oil, vegetable fat powder, phytosterol ester, phytostanol ester, DHA algae oil, arachidonic acid grease, maltodextrin, low or polyfructose, galacto-oligosaccharide, polydextrose, glucose, corn syrup solid, isomalto-oligosaccharide, inulin, lactoferrin, casein phosphopeptide, chitosan oligosaccharide, soybean oligosaccharide, beta-glucan, beta-hydroxy-beta-methylbutyric acid, amino acid, soybean protein isolate, papaya powder, pumpkin powder, milk mineral salt, banana powder, blueberry powder, acerola powder, Strawberry powder, fructus Mali Pumilae, fructus Hippophae, fructus Lycii, fructus Pruni Pseudocerasi, fructus litchi, semen Sesami Niger, rhizoma Dioscoreae, semen Pini, milk, or herba Nelumbinis pistil liquid or processed powder or salt compound of different raw materials.

The formula milk powder is characterized by further comprising taurine, lutein, L-carnitine, vitamin A, beta carotene, vitamin D, vitamin K, vitamin B1, vitamin B2, vitamin B6, vitamin B12, nicotinic acid, folic acid, pantothenic acid, biotin, choline, a selenium agent, a calcium agent, an iron agent, a zinc agent, a magnesium agent, a copper agent, a manganese agent, a potassium agent and a phosphorus agent. The selenium agent is selected from one or more of sodium selenite, sodium selenate, selenoprotein, selenium-rich edible fungus powder, and L-selenium-methyl selenocysteine; the calcium agent is selected from one or more of calcium carbonate, calcium gluconate, calcium citrate, calcium lactate, L-calcium lactate, calcium hydrogen phosphate, calcium L-threonate, calcium glycinate, calcium aspartate, calcium citrate malate, calcium acetate (calcium acetate), calcium chloride, tricalcium phosphate, calcium vitamin E succinate, calcium glycerophosphate, calcium oxide, calcium sulfate, and bone meal; the iron agent is selected from one or more of ferrous sulfate, ferrous gluconate, ferric ammonium citrate, ferrous fumarate, ferric citrate, ferrous lactate, hemin, ferric pyrophosphate, ferriporphyrin, ferrous glycinate, reduced iron, sodium ferric ethylenediamine tetraacetate, carbonyl iron powder, ferrous carbonate, ferrous citrate, ferrous fumarate, ferrous succinate, heme iron and electrolytic iron; the zinc agent is selected from one or more of zinc sulfate, zinc gluconate, zinc glycinate, zinc oxide, zinc lactate, zinc citrate, zinc chloride, zinc acetate and zinc carbonate; the magnesium agent is selected from one or more of magnesium sulfate, magnesium chloride, magnesium oxide, magnesium carbonate, magnesium hydrogen phosphate and magnesium gluconate; the copper agent is selected from one or more of copper sulfate, copper gluconate, copper citrate and copper carbonate; the manganese agent is selected from one or more of manganese sulfate, manganese chloride, manganese carbonate, manganese citrate and manganese gluconate; the potassium agent is selected from potassium chloride, potassium carbonate, potassium bicarbonate, potassium lactate, potassium citrate, potassium gluconate, potassium dihydrogen phosphate and dipotassium hydrogen phosphate; the potassium agent is one or more selected from tricalcium phosphate and calcium hydrogen phosphate.

The components are prepared by dry method preparation of milk powder or dry-wet method composite technology of milk powder to prepare a sample.

Detailed Description

In order to make the objects, technical solutions and effects of the present invention clearer and clearer, the present invention is further described in detail below with reference to specific embodiments. It should be understood that the specific embodiments described herein are merely illustrative of the invention and are not intended to limit the invention.

Example 1

The formula milk powder is prepared from the following components in parts by weight: 99970 parts of milk solid composition, 10 parts of hyaluronic acid composition and 20 parts of vitamin E.

The milk solid composition comprises 49970 parts of whole milk powder and 50000 parts of skimmed milk powder.

The hyaluronic acid composition is sodium hyaluronate.

The above components were mixed uniformly or prepared by dry-wet compounding process to obtain comparative sample 2.

Example 2

The formula milk powder is prepared from the following components in parts by weight: 84990 parts of milk solid composition, 10 parts of hyaluronic acid composition and 15000 parts of collagen composition.

The milk solid composition comprises 34990 parts of whole milk powder and 50000 parts of skimmed milk powder. .

The hyaluronic acid composition is sodium hyaluronate.

The collagen composition is collagen.

The above components were mixed uniformly or prepared by dry-wet compounding process to obtain comparative sample 1.

Example 3

The formula milk powder is prepared from the following components in parts by weight: 84910 parts of milk solid composition, 10 parts of hyaluronic acid composition, 15000 parts of collagen composition and 70 parts of vitamin C composition.

The milk solid composition comprises 34910 parts of whole milk powder and 50000 parts of skimmed milk powder.

The hyaluronic acid composition is sodium hyaluronate.

The collagen composition is collagen.

The vitamin C composition is sodium L-ascorbate.

The above components were mixed uniformly or prepared by dry-wet compounding process to obtain comparative sample 3.

Example 4

The composition is prepared from the following components in parts by weight: 84970 parts of milk solid composition, 10 parts of hyaluronic acid composition, 15000 parts of elastin composition and 20 parts of vitamin E.

The milk solid composition comprises 34970 parts of whole milk powder and 50000 parts of skimmed milk powder.

The hyaluronic acid composition is sodium hyaluronate.

The elastin composition is skipjack elastin peptide.

The above components are mixed uniformly or prepared by a dry-wet process composite process to prepare a sample 4.

Example 5

The formula milk powder for beautifying and resisting aging is prepared from the following components in parts by weight: 70000 parts of milk solid composition, 10 parts of hyaluronic acid composition, 14900 parts of elastin composition, 15000 parts of collagen composition, 20 parts of vitamin E and 70 parts of vitamin C composition.

The milk solid composition is a whole milk powder.

The hyaluronic acid composition is sodium hyaluronate.

The elastin composition is skipjack elastin peptide.

The collagen composition is collagen.

The vitamin C composition is sodium L-ascorbate.

The above components are mixed uniformly or prepared by a dry-wet process composite process to prepare a sample 5.

The effects of the present invention are further illustrated by comparative experiments as follows:

comparative example 1

The composition is prepared from the following components in parts by weight: 99990 parts of milk solid composition, 20 parts of vitamin E and 70 parts of vitamin C composition.

The milk solid composition comprises 49990 parts of whole milk powder and 50000 parts of skimmed milk powder.

The vitamin C composition is sodium L-ascorbate.

The components are mixed evenly or prepared by a dry-wet method composite process to prepare a comparative sample 1

Comparative example 2

The composition is prepared from the following components in parts by weight: 70000 parts of milk solid composition, 20 parts of vitamin E and 70 parts of vitamin C composition.

The milk solid composition comprises 20000 parts of whole milk powder and 50000 parts of skimmed milk powder.

The vitamin C composition is sodium L-ascorbate.

The component also comprises 29910 parts of maltodextrin.

The above components were mixed uniformly or prepared by dry-wet compounding process to obtain comparative sample 2.

Comparative example 3

The composition is prepared from the following components in parts by weight: 29910 parts of milk solid composition, 20 parts of vitamin E and 70 parts of vitamin C composition.

The milk solid composition is 29910 parts of whole milk powder.

The vitamin C composition is sodium L-ascorbate.

The component also comprises 70000 parts of maltodextrin.

The above components were mixed uniformly or prepared by dry-wet compounding process to obtain comparative sample 2.

Samples the compositions or formula powders prepared according to the methods of examples 1, 2, 3, 4, 5 and comparative examples 1, 2, 3.

Firstly, comparing sensory experiments.

As can be seen from the first table, the sensory indexes of the samples prepared in examples 1, 2, 3, 4 and 5 and comparative examples 1 and 2 are all acceptable, and the sensory index of the sample prepared in comparative example 3 is not acceptable.

Test animals and groups: mice representative of healthy males were selected. Randomly dividing the mice into 3 big groups according to the body weight, wherein 90 mice in the group I are divided into 9 dose groups, and 10 mice in each group are subjected to hydroxyproline content experiment in the skin of the mice; II, dividing 90 mice into 9 dose groups, and measuring the content of superoxide dismutase (SOD) and Malondialdehyde (MDA) in the skin of the mice, wherein each group comprises 10 mice; group III mice 90, divided into 9 dose groups, each group of 10, were compared for apparent characteristics of the skin.

The samples of the present invention were fed to samples 1, 2, 3, 4, 5 and comparative samples 1, 2, 3 at a dose of 1g/kg · bw, respectively, with reference to the recommended human dose of 120 g/human/day (based on a weight of 60 kg), and the group of 1g/kg · bw was fed rice as a model group control.

Attached: the 90 experimental mice were randomly divided into 9 groups:

(1) model group control, subcutaneous injection of D-galactose, with rice feeding.

(2) Sample 1 group, D-galactose was injected subcutaneously while feeding sample 1.

(3) Sample 2 group, s.c. injected with D-galactose and fed sample 2.

(4) Sample 3 group, administered with D-galactose subcutaneously, was fed with sample 3.

(5) Sample 4 group, administered with D-galactose subcutaneously, was fed with sample 4.

(6) Sample 5 group, administered with D-galactose subcutaneously, was fed with sample 5.

(7) Comparative sample 2 group, D-galactose was injected subcutaneously while feeding comparative sample 1.

(8) Comparative sample 2 group, D-galactose was injected subcutaneously while feeding comparative sample 2.

(9) Comparative sample 2 group, D-galactose was injected subcutaneously while feeding comparative sample 3.

After each group of mice was raised for one week, the experiment was started. All mice were dehaired with 6% sodium sulfide, exposing 3X 3cm of back²The size of the skin. According to the experimental method, the experiment was terminated after the mice were raised for 3 months. After one day of fasting, the skin was cleaned and the mice were sacrificed by cervical dislocation. The removed skin was stored frozen and used for each index test. Sample effect on hydroxyproline content of mouse skin.

Secondly, measuring the content of hydroxyproline.

Hydroxyproline is a characteristic amino acid of collagen, and the content of hydroxyproline can indirectly reflect the content of collagen. As the organism ages, hydroxyproline in the collagen fibers forms cross-links in molecules, and the activity of proline hydroxylase is reduced due to the cross-linking of the bridges among macromolecules, so that the hydroxylation of proline is weakened, the hydroxyproline in the skin collagen fibers is reduced, the synthesis of the collagen fibers is insufficient, the elasticity of the skin is reduced, the moisture is reduced, and wrinkles are thick and deep.

The experimental result shows that the content of hydroxyproline in the skin of mice in the groups of samples 1, 2, 3, 4 and 5 and the model group is obviously improved, wherein the content sequence of hydroxyproline in the sample group is as follows: example 5 sample set > example 3 sample set > example 4 sample set > example 2 sample set > example 1 sample set > comparative example 2 sample set > comparative example 3 sample set, demonstrating the significant improvement in collagen synthesis ability of aged skin upon consumption of the inventive samples. Compared with the comparative sample groups 1, 2 and 3, the hydroxyproline content in the skin of the mouse is improved, which shows that the formula can greatly enhance collagen to prevent the loss of collagen in the skin through the synergistic effect of different components.

And thirdly, the effects on the mouse skin over superoxide dismutase (SOD) and Malondialdehyde (MDA).

With the age, the antioxidant enzymes in the body are reduced, the defense function is reduced or impaired, so that the accumulation of free radicals is increased, various macromolecules in skin cells are damaged, and the phenomena of dryness, wrinkles, lackluster, mass deposition of pigments and the like of the skin are caused. Aging skin generates a large amount of free radicals, which have strong oxidizing property, can initiate peroxidation of unsaturated lipids in biological membranes to form lipid peroxides, and generate Malondialdehyde (MDA), a strong cross-linking agent. Malondialdehyde can combine with biological macromolecules such as protein, nucleic acid, and lipid to form insoluble substances, which can cause damage to various macromolecules in the body. The superoxide dismutase (SOD) is the most important antioxidant enzyme for human body, and can effectively remove superoxide radical in vivo and inhibit the production of malondialdehyde. Thus, over the rise in superoxide dismutase, malondialdehyde reduces the indirect reaction and improves the anti-aging effect.

The aging causes the SOD enzyme activity in the skin of the model group mice to be obviously reduced, the MDA content is obviously increased, and the difference is obvious compared with that of the normal group mice. Compared with the model group, each experimental group shows that the SOD enzyme activity is obviously improved, the MDA content is obviously reduced, wherein the SOD enzyme activity in the sample groups 1, 2, 3, 4 and 5 is obviously higher than that in the sample groups 1, 2 and 3, and the Malondialdehyde (MDA) content is obviously lower than that in the sample groups 1, 2 and 3. The SOD enzyme activity has the following sequence: example 5 sample set > example 4 sample set > example 3 sample set > example 2 sample set > example 1 sample set > comparative example 2 sample set > comparative example 1 sample set > comparative example 3 sample set > model comparative blank set. The MDA content has the following size sequence: with example 5 sample set < example 4 sample set < example 3 sample set < example 2 sample set < example 1 sample set < comparative example 3 sample set < comparative example 2 sample set < comparative example 1 sample set < model comparative blank set. The sample set of example 5 had better SOD enzyme activity and lower MDA content, indicating that the sample set of example 5 had the best anti-aging effect, and next, the sample set of example 4, the sample set of example 3, the sample set of example 2, and the sample set of example 1.

And fourthly, apparent characteristics of the skin of the mouse.

After the experiment is finished, the appearance characteristics of the skin of each group of mice are observed, and the characteristics are shown in the fourth table.

As can be seen from the comparison of Table IV, the skin of the model group mice is dark and dull, rough and wrinkled, and becomes loose and inelastic. The comparative example group had little wrinkles, weak elasticity, loose epidermis, and few wrinkles. While the example group showed a ruddy, smooth, elastic, firm epidermis. In particular, the group of samples of example 5 had a red, smooth, elastic, firm epidermis and no wrinkles.

Through comprehensive experiments of the first, second, third and fourth tables, the skin-nourishing and anti-aging effects of the example groups are higher than those of the comparative example groups and than those of the model comparative blank groups. Example 5 sample group, showed the best skin-beautifying anti-aging effect.

The above detailed description section specifically describes the analysis method according to the present invention. It should be noted that the above description is only for the purpose of helping those skilled in the art better understand the method and idea of the present invention, and not for the limitation of the related contents. The present invention may be appropriately adjusted or modified by those skilled in the art without departing from the principle of the present invention, and the adjustment and modification also fall within the scope of the present invention.