阅读说明：本技术 包含亚油酸的组合物 (Compositions comprising linoleic acid ) 是由 A·沃森 D·阿拉韦 G·托马斯 于 2019-09-10 设计创作，主要内容包括：本发明涉及包含7g/Mcal至9g/Mcal范围内的量的亚油酸的饮食或食料用于维持或改善健康宠物的皮肤质量的用途。在一些实施方式中,饮食或食料还包含约50mg/Mcal的量的锌。本发明还涉及包含7g/Mcal至9g/Mcal范围内的量的亚油酸的饮食或食料用于治疗罹患皮肤疾病或病症的宠物动物的方法。(The present invention relates to the use of a diet or foodstuff comprising linoleic acid in an amount in the range of from 7g/Mcal to 9g/Mcal for maintaining or improving the skin quality of healthy pets. In some embodiments, the diet or foodstuff further comprises zinc in an amount of about 50 mg/Mcal. The invention also relates to a method of treating a pet animal suffering from a skin disease or condition with a diet or foodstuff comprising linoleic acid in an amount in the range of from 7g/Mcal to 9 g/Mcal.)

1. A method of maintaining or improving skin quality in a healthy pet animal, the method comprising feeding the healthy pet animal a diet or foodstuff comprising linoleic acid in an amount in the range of from 7g/Mcal to 9 g/Mcal.

2. The method of claim 1, wherein linoleic acid is present in an amount of 8 g/Mcal.

3. The method according to claim 1, wherein the diet or foodstuff comprises one or more sources of linoleic acid selected from the group consisting of safflower oil, sunflower oil, soybean oil, sesame oil, canola oil, meat, and combinations of two or more thereof.

4. The method according to claim 1, wherein the diet or foodstuff further comprises zinc in an amount ranging from 40mg/Mcal to 60 mg/Mcal.

5. The method according to claim 1, wherein the diet or foodstuff further comprises zinc in an amount of 50 mg/Mcal.

6. The method according to claim 1 wherein the diet or foodstuff comprises one or more zinc sources selected from the group consisting of zinc sulfate, zinc oxide, organozinc complexes such as chelated zinc, zinc orotate, zinc picolinate, zinc gluconate, zinc acetate, and combinations of two or more thereof.

7. The method of claim 1 wherein transepidermal water loss (TEWL) is reduced in a healthy pet animal.

8. The method of claim 1, wherein the healthy pet animal is a healthy dog.

9. A diet or foodstuff as defined in any one of claims 1 to 8.

10. A method of treating a skin disease or condition in a pet animal in need thereof, the method comprising feeding the pet animal a diet or foodstuff comprising linoleic acid in an amount in the range of from 7g/Mcal to 9 g/Mcal.

11. The method of claim 10, wherein the skin disease or disorder is selected from the group consisting of dermatitis, psoriasis, atopic dermatitis, skin forms of food allergies, pruritic diseases, bacterial folliculitis, and furunculosis.

12. The method of claim 10, wherein linoleic acid is present in an amount of 8 g/Mcal.

13. The method according to claim 10, wherein the diet or foodstuff comprises one or more sources of linoleic acid selected from the group consisting of safflower oil, sunflower oil, soybean oil, sesame oil, canola oil, meat, and combinations of two or more thereof.

14. The method according to claim 10, wherein the diet or foodstuff further comprises zinc in an amount ranging from 40mg/Mcal to 60 mg/Mcal.

15. The method according to claim 10, wherein the diet or foodstuff further comprises 50mg/Mcal of zinc.

16. The method according to claim 10 wherein the diet or foodstuff comprises one or more zinc sources selected from the group consisting of zinc sulfate, zinc oxide, organozinc complexes such as chelated zinc, zinc orotate, zinc picolinate, zinc gluconate, zinc acetate, and combinations of two or more thereof.

17. The method of claim 10 wherein TEWL is decreased in the pet animal.

18. The method of claim 10, wherein the pet animal is a dog.

19. Use of a diet or foodstuff comprising linoleic acid in an amount in the range of from 7g/Mcal to 9g/Mcal in maintaining or improving the skin quality of a healthy pet animal.

20. The use of claim 19, wherein linoleic acid is present in an amount of 8 g/Mcal.

21. The use according to claim 19 or 20, wherein the diet or foodstuff comprises one or more sources of linoleic acid selected from the group consisting of safflower oil, sunflower oil, soybean oil, sesame oil, canola oil, meat, and combinations of two or more thereof.

22. Use according to any one of claims 19 to 21, wherein the diet or foodstuff further comprises zinc in an amount in the range of from 40mg/Mcal to 60 mg/Mcal.

23. The use according to claim 22, wherein the diet or foodstuff comprises zinc in an amount of 50 mg/Mcal.

24. The use according to any one of claims 19 to 23 wherein the diet or foodstuff comprises one or more zinc sources selected from the group consisting of zinc sulfate, zinc oxide, organozinc complexes such as chelated zinc, zinc orotate, zinc picolinate, zinc gluconate, zinc acetate, and combinations of two or more thereof.

25. The use of any one of claims 19 to 24, wherein TEWL is reduced in the pet animal.

26. The use according to any one of claims 19 to 25, wherein the healthy pet animal is a healthy dog.

27. A method of maintaining or improving skin quality in a healthy pet animal, the method comprising feeding the healthy pet animal with the diet or foodstuff of any one of claims 19 to 26.

28. The method of claim 27 wherein TEWL is reduced in the healthy pet animal.

29. The method of claim 27 or 28, wherein the healthy pet animal is a healthy dog.

30. A diet or foodstuff comprising linoleic acid in the range of 7 to 9g/Mcal for use in a method of treating a pet animal suffering from a skin disease or condition.

31. The diet or foodstuff for use in said method according to claim 30, wherein said skin disease or condition is selected from the group consisting of dermatitis, psoriasis, atopic dermatitis, food-allergic skin forms, pruritic diseases, bacterial folliculitis, and furunculosis.

32. The diet or foodstuff for use in a method according to claim 30 or 31, wherein linoleic acid is present in an amount of 8 g/Mcal.

33. The diet or foodstuff for use in the method according to any one of claims 30 to 32, wherein the diet or foodstuff comprises one or more sources of linoleic acid selected from the group consisting of safflower oil, sunflower oil, soybean oil, sesame oil, canola oil, meat, and combinations of two or more thereof.

34. A diet or foodstuff for use in the method according to any one of claims 30 to 33, wherein the diet or foodstuff further comprises zinc in an amount in the range of 40 to 60 mg/Mcal.

35. A diet or foodstuff for use in the method according to claim 34, wherein the diet or foodstuff comprises zinc in an amount of 50 mg/Mcal.

36. The diet or foodstuff for use in the method according to any one of claims 30 to 36, wherein the diet or foodstuff comprises one or more zinc sources selected from the group consisting of zinc sulfate, zinc oxide, organozinc complexes such as chelated zinc, zinc orotate, zinc picolinate, zinc gluconate, zinc acetate, and combinations of two or more thereof.

37. The diet or foodstuff for use in a method according to any one of claims 30 to 36, wherein TEWL is reduced in said pet animal.

38. The diet or foodstuff for use in the method according to any one of claims 30 to 37, wherein the pet animal is a dog.

Technical Field

The present disclosure relates to the field of compositions comprising linoleic acid for use in improving skin quality in healthy pet animals or for treating pet animals suffering from diseases causing skin diseases (skin disorders) or dermatological disorders (dermatological disorders).

Background

Skin-related problems are the most commonly encountered problems in cats and dogs, causing great anxiety and resulting in significant time and effort to solve. This is why maintaining the skin of the pet in an optimal state can be considered as a goal to be achieved by the pet owner. As the skin and coat condition of pets provides important visual effects, there is great interest in providing visual enhancement in animals already having healthy skin and coat conditions. The skin of pets, including dogs, is thinner than human skin, thus making them susceptible to skin discomfort. Thus, enhancing skin condition is beneficial to pets because skin helps prevent dehydration and resists changes in ambient temperature.

During periods of nutritional deficiency, the health of the skin and hair, and thus the visual appearance of the pet's hair, may be compromised.

Dietary factors are known in the art to play a major role in maintaining the healthy coat and skin of animal pets. It is well documented that the levels of nutrients in pet food can provide a complete and balanced diet for healthy animals. For example, dietary zinc is considered to be an important element involved in healthy development of coat and skin. The presence of zinc is particularly important in rapidly splitting skins (Watson, 1998, J Nutr, Vol. 128 (suppl. 12): 27835-. Linoleic acid is also important for skin and hair conditions. Notably, dogs are unable to synthesize this fatty acid and therefore are essential for dietary sources. Fatty acids are important for maintaining cell membrane fluidity and the water barrier of skin permeability (Campbell, 1990, North American veterinary clinical animal practice (Vet Clin North Am Anim practice.), Vol.29 (6): 1373-1383). A variety of other nutrients, including vitamin B, also play a role in skin condition (Watson et al, 2006, Experimental dermatology (Exp Dermatol), Vol. 15 (1): 74-81).

It has been shown in the art that skin and coat condition can be improved by providing a diet comprising higher amounts of linoleic acid and zinc than are present in conventional pet foods. It is noted that U.S. patent No. 6,331,567 discloses a foodstuff for improving the condition of pet skin comprising a combination of zinc and linoleic acid. This prior art document discloses the testing of skin and coat condition in dogs on control and test diets comprising respectively: (i)1.8g/400kcal or 6g/kcal linoleic acid and (ii)12.5mg/400kcal or 40mg/400kcal zinc. A significant increase in shine of the coat and improvement in skin scaling was observed in the test diet compared to the control diet. For the test diets, a reduction in the transdermal water loss of the dog skin was also determined. Reduction of transdermal water loss can also be achieved with a diet supplemented only with zinc, so that the addition of linoleic acid is not required. Excellent improvement of skin and hair condition properties was also obtained with a diet containing a lower amount of zinc, i.e. a diet containing 6g/kcal linoleic acid and 25mg/400kcal zinc.

There remains a need in the art for pet diets, including dog diets, that allow for the maintenance or improvement of skin condition in healthy pet animals.

As previously mentioned, healthy skin and coat indicates that the animal is generally physically healthy in most domestic pets.

However, pet animals may in some cases be affected by dermatological disorders. Therefore, improving skin and hair conditions in pet animals is also a continuing goal in the art, particularly where the pet animal is suffering from a skin disease.

For example, dog skin disease is the only most common cause of pets being brought to veterinary clinics. The proportion of animals suffering from such diseases is believed to be about 15% of the routine work of such clinics. The main diseases that led to this statistic are flea allergic dermatitis (flea allergic dermatitis), atopic dermatitis, pyoderma (pyoderma) (bacterial infection), seborrheic dermatitis (seborrhea), and mange (mange) (demodex and sarcoptian). In most of these skin diseases, the barrier properties of the skin are greatly diminished, leading in particular to a change in the transdermal water loss.

Thus, in a significant aspect, there is also a need in the art for pet compositions that are capable of treating pet animals suffering from a skin disease or disorder.

Disclosure of Invention

The present disclosure relates to the use of a diet or foodstuff comprising linoleic acid in an amount in the range of from about 7 grams per megacard (g/Mcal) to about 9g/Mcal for maintaining or improving skin quality in a pet. In some embodiments, the diet or foodstuff comprises linoleic acid in an amount ranging from 7g/Mcal to 9 g/Mcal.

In some embodiments, linoleic acid is present in an amount of about 8g/Mcal, e.g., 8 g/Mcal.

In some embodiments, the diet or foodstuff comprises one or more sources of linoleic acid selected from, for example, the group consisting of safflower oil, sunflower oil, soybean oil, sesame oil, canola oil, meat, and combinations of two or more thereof.

In some embodiments, the diet or foodstuff further comprises zinc in an amount in the range of about 40 milligrams per megacalorie (mg/Mcal) to about 60mg/Mcal, for example in an amount in the range of 40mg/Mcal to 60 mg/Mcal. In a particular embodiment, the diet or foodstuff further comprises zinc in an amount of about 50mg/Mcal, for example 50 mg/Mcal.

In some embodiments, the diet or foodstuff comprises one or more zinc sources selected from, for example, the group consisting of zinc sulfate, zinc oxide, organozinc complexes such as chelated zinc, zinc orotate, zinc picolinate, zinc gluconate, zinc acetate, and combinations of two or more thereof.

In some embodiments, the pet is a dog.

The present disclosure also relates to a method for maintaining or improving skin quality in a pet animal, in particular a dog, comprising the step of feeding the pet animal with a diet or foodstuff described in the present disclosure.

The present disclosure also relates to a diet or foodstuff comprising linoleic acid in an amount ranging from about 7g/Mcal to about 9g/Mcal for maintaining or improving skin quality in a pet animal. In some embodiments, the diet or foodstuff comprises linoleic acid in an amount from 7g/Mcal to 9 g/Mcal. In some embodiments, the diet or foodstuff comprises linoleic acid in an amount of about 8g/Mcal, for example 8 g/Mcal.

In some embodiments, the diet or foodstuff further comprises zinc in an amount ranging from about 40mg/Mcal to about 60mg/Mcal, for example 40mg/Mcal to 60 mg/Mcal. In a particular embodiment, the diet or foodstuff further comprises about 50mg/Mcal, for example 50mg/Mcal, of zinc.

In some embodiments, the diet or foodstuff is a dog diet or foodstuff.

The present disclosure also relates to a diet or foodstuff comprising linoleic acid in an amount ranging from about 7g/Mcal to about 9g/Mcal for use in a method of treating a pet animal afflicted with a skin disease or disorder. In some embodiments, the diet or foodstuff comprises linoleic acid in an amount in the range of 7g/Mcal to 9g/Mcal for use in a method of treating a pet animal afflicted with a skin disease or disorder.

The present disclosure also relates to the use of linoleic acid in an amount in the range of from about 7g/Mcal to about 9g/Mcal, for example in an amount of from 7g/Mcal to 9g/Mcal, in the manufacture of a pharmaceutical diet or foodstuff for treating a dog suffering from a skin disease or disorder.

The present disclosure also relates to a method of maintaining or improving skin quality in a healthy pet animal comprising feeding the healthy pet animal a diet or foodstuff comprising linoleic acid in an amount ranging from about 7g/Mcal to about 9g/Mcal, for example in an amount from 7g/Mcal to 9 g/Mcal.

In some embodiments, linoleic acid is present in an amount of about 8g/Mcal, e.g., 8 g/Mcal. In some embodiments, the diet or foodstuff comprises one or more sources of linoleic acid selected from the group consisting of safflower oil, sunflower oil, soybean oil, sesame oil, canola oil, meat, and combinations of two or more thereof.

In some embodiments, the diet or foodstuff further comprises zinc in an amount ranging from about 40mg/Mcal to about 60mg/Mcal, for example 40mg/Mcal to 60 mg/Mcal. In some embodiments, the diet or foodstuff further comprises about 50mg/Mcal, for example 50mg/Mcal, of zinc. In some embodiments, the diet or foodstuff comprises one or more zinc sources selected from the group consisting of zinc sulfate, zinc oxide, organozinc complexes such as chelated zinc, zinc orotate, zinc picolinate, zinc gluconate, zinc acetate, and combinations of two or more thereof.

In certain embodiments, the transepidermal water loss (TEWL) is reduced in the healthy pet animal. In a particular embodiment, the healthy pet animal is a healthy dog.

The present disclosure also describes a method of treating a skin disease or condition in a pet animal in need thereof, the method comprising feeding the pet animal a diet or foodstuff comprising linoleic acid in an amount ranging from about 7g/Mcal to about 9g/Mcal, for example in an amount from 7g/Mcal to 9 g/Mcal. In some embodiments, the skin disease or disorder is selected from the group consisting of dermatitis (dermatitis), psoriasis (psoriasis), atopic dermatitis (atopic dermatitis), food-allergic skin forms (cutaneous forms of food allergy), pruritis (pruritic diseases), bacterial folliculitis (bacterial folliculitis), and furunculosis (furinuosis).

In some embodiments, linoleic acid is present in an amount of about 8g/Mcal, e.g., 8 g/Mcal. In some embodiments, the diet or foodstuff comprises one or more sources of linoleic acid selected from the group consisting of safflower oil, sunflower oil, soybean oil, sesame oil, canola oil, meat, or a combination of two or more thereof.

In some embodiments, the diet or foodstuff further comprises zinc in an amount in the range of about 40mg/Mcal to about 60mg/Mcal, for example 40mg/Mcal to 60 mg/Mcal. In certain embodiments, the diet or foodstuff further comprises about 50mg/Mcal, e.g., 50mg/Mcal, of zinc. In some embodiments, the diet or foodstuff comprises one or more zinc sources selected from the group consisting of zinc sulfate, zinc oxide, organozinc complexes such as chelated zinc, zinc orotate, zinc picolinate, zinc gluconate, zinc acetate, or combinations of two or more thereof.

In certain embodiments, TEWL is decreased in the pet animal. In a particular embodiment, the pet animal is a dog.

The foregoing has outlined rather broadly the features and technical advantages of the present application in order that the detailed description that follows may be better understood. Additional features and advantages of the application will be described hereinafter which form the subject of the claims of the application. It should be appreciated by those skilled in the art that the conception and specific embodiment disclosed may be readily utilized as a basis for modifying or designing other structures for carrying out the same purposes of the present application. It should also be realized by those skilled in the art that such equivalent constructions do not depart from the spirit and scope of the application as set forth in the appended claims. The novel features which are believed to be characteristic of the application, both as to its organization and method of operation, together with further objects and advantages will be better understood from the following description.

Drawings

Figure 1 shows the results of measuring the trans-dermal water loss (TEWL) of dogs fed different types of food. Ordinate: TEWL in grams per hour per square meter (g/hr/m)²) And (4) showing. The abscissa: (A) dogs fed diet a; (B) dogs fed diet B; (C) dogs fed diet C; (D) dogs fed diet D. For each of groups a, B, C and D, the time points after the start of feeding the dogs with the indicated diet are expressed in weeks.

Detailed Description

The inventors have shown that an optimal improvement in the skin health of a healthy pet animal can be obtained by providing a diet or foodstuff comprising linoleic acid in a specific amount range to a healthy pet animal, in particular a healthy dog. More precisely, the inventors found that an improvement in the skin quality, i.e. a reduced trans-epidermal water loss (TEWL), of healthy pet animals is obtained by providing linoleic acid to the animals, in particular to healthy dogs, in a specific amount range.

Incidentally, in some cases, the improvement in skin quality may result in beneficial effects on body coat(s), such as beneficial effects on the shine, smoothness and softness of body coat(s).

As is well known in the art, transdermal moisture loss (TEWL) is defined as the amount of water that is transferred from inside the body to outside the body through the epidermal layer (epidermal layer) (see, e.g., du Plessis J, Stefaniak A, Eloff F, John S, Agner T, Chou TC, Nixon R, Steiner M, Franken A, Kudla I, HolnessL. International guidelines for the in vivo assessment of Skin properties in non-clinical settings: Part 2 (International guidelines for in vivo assessment of Skin properties in non-clinical settings). transdermal water dispersion and Skin hydration. Skin research and technology (Skin Res Technol.)2013 Aug; 19(3): 265-78).

The present disclosure relates to a diet or foodstuff, in particular for healthy pet animals, comprising linoleic acid in an amount ranging from 7g/Mcal to 9 g/Mcal. The present disclosure also relates to the use of a diet or foodstuff comprising linoleic acid in an amount in the range of from 7g/Mcal to 9g/Mcal for maintaining or improving the skin quality of a healthy pet animal, in particular for maintaining or improving the skin quality of a healthy pet animal.

As used herein, the term "diet" or "foodstuff (foodstuff)" refers to a substance comprising proteins, carbohydrates and/or fats, which are used in the organism to maintain growth, repair and vital processing and provide energy. The foodstuff may also contain supplementary substances or additives such as minerals, vitamins and condiments (see university of Welch Dictionary (Merriam-Webster's Collegiate Dictionary), 10 th edition, 1993). In a preferred embodiment, the diet or foodstuff according to the present disclosure consists of a nutritionally complete diet or foodstuff, which provides a complete and balanced nutritional requirement for the pet animal. Thus, the diet or foodstuff described herein is a complete pet animal food, such as a complete dog food, i.e., a nutritionally sufficient feed that can be fed to a pet animal, such as a dog, as a sole ration and is capable of sustaining life without the need for additional food (other than water).

The diet or foodstuff may comprise a carrier, diluent or excipient. Depending on the intended use, carriers, diluents or excipients suitable for use in animals, particularly pet animals such as dogs and cats, may be selected.

As used herein, a "pet animal" consists of a pet mammal. Pet mammals include dogs, cats, rabbits, hamsters, guinea pigs (guineapig), rats, and mice. Preferred pet animals herein are dogs and cats.

As used herein, "maintaining skin quality" refers to maintaining skin quality at a substantially stable level over time, e.g., maintaining a transdermal moisture loss level at a substantially stable level over time.

As used herein, "improving skin quality" refers to achieving a measurable improvement in skin quality, such as achieving a measurable reduction in transdermal moisture loss. The term "skin quality" may refer to skin properties such as skin hydration, skin texture, skin elasticity, skin integrity, skin barrier. The person skilled in the art will select a known method of measuring skin quality improvement (as described in the examples). Illustratively, as shown in the examples herein, improvements in skin condition or skin quality can be assessed by measuring the reduction in transcutaneous water loss (TEWL).

To assess the improvement in skin quality of a test diet or foodstuff, a variety of healthy animal populations have been fed different diets or foodstuffs, each comprising a given amount of linoleic acid, and then the skin quality (e.g., TEWL) was measured (i) the start time before feeding the given foodstuff, and (ii) the successive time intervals of the given foodstuff during feeding. Experimental results show that a substantial improvement in skin quality (e.g., a substantial reduction in TEWL) is obtained with a foodstuff comprising linoleic acid in an amount in the range of from about 7g/Mcal to about 9g/Mcal, such as from 7g/Mcal to 9 g/Mcal. Furthermore, by comparing the different foodstuffs tested with each other, it was found that the most effective foodstuff for which a significant improvement in skin quality has been measured is a foodstuff comprising linoleic acid in an amount in the range of from about 7g/Mcal to about 9g/Mcal, such as 7g/Mcal to 9 g/Mcal.

As used herein, the expression "x g/Mcal" for a given substance contained in a diet or foodstuff means that the substance is included in an amount of x grams per Mcal contained in the foodstuff diet.

The inventors' results also indicate that a diet or foodstuff comprising linoleic acid at 10g/Mcal or more does not provide any measurable improvement in skin quality, in particular does not significantly reduce transepidermal water loss (TEWL). The latter finding is even more surprising, as the prior art disclosed in U.S. patent No. 6,331,567 teaches that skin condition can be improved by a dietary diet comprising at least 4g/400kcal linoleic acid (i.e. at least 10g/Mcal linoleic acid), such as 7g/400kcal (i.e. 17.5 g/Mcal).

It has also been found herein that a diet or foodstuff comprising linoleic acid at 6g/Mcal or less does not provide any significant improvement in skin quality, in particular does not provide any significant reduction in trans-dermal water loss (TEWL).

In some embodiments, the diet or foodstuff comprises about 8g/Mcal linoleic acid. In some embodiments, the diet or foodstuff comprises linoleic acid at 8 g/Mcal.

Linoleic acid may be present in varying amounts in a variety of substances comprising linoleic acid in the diet or foodstuff described herein. However, in a preferred embodiment, the diet or foodstuff comprises one or more substances in which linoleic acid is present in a relatively high amount. Thus, in some embodiments, the diet or foodstuff described herein comprises one or more linoleic acid derived materials, i.e., linoleic acid containing materials.

The preferred linoleic acid source, i.e., linoleic acid containing material, is preferably a vegetable oil, although animal oils or fats may also be used. Preferred sources of linoleic acid, i.e., linoleic acid containing material, include safflower oil, sunflower oil, soybean oil, sesame oil, canola oil, other vegetable or animal oils/fats, meat, or combinations of two or more thereof.

Most preferably, the linoleic acid source, i.e. the linoleic acid containing material, comprised in the diet or foodstuff described herein is selected from the group comprising safflower oil, sunflower oil, soybean oil or a combination of two or more thereof.

In some preferred embodiments, the diet or foodstuff further comprises zinc.

In the diet or foodstuff described herein, zinc can be present in variable amounts in a variety of substances in which it is contained. However, in a preferred embodiment, the diet or foodstuff further comprises one or more substances in which zinc is present in substantial amounts, for example in the form of a zinc salt or zinc complex.

As shown in the examples, the diet or foodstuff disclosed herein comprises a specific amount of linoleic acid and additionally a certain amount of zinc. Zinc can be present in an amount from about 40mg/Mcal to about 60mg/Mcal, such as from 40mg/Mcal to 60 mg/Mcal. In some embodiments, the zinc is present in an amount of about 50mg/Mcal, for example 50 mg/Mcal. The diet or foodstuff disclosed herein comprising a specific amount of linoleic acid and additionally an amount of zinc, for example in the amounts disclosed herein, may provide a measurable improvement in skin quality, for example a significant reduction in TEWL.

In some embodiments, the diet or foodstuff described herein comprises both linoleic acid and zinc in amounts greater than that contained in a conventional diet or foodstuff for a pet animal, particularly a dog or cat diet or foodstuff, and particularly a dog's foodstuff diet. Thus, in some embodiments, the foodstuff diet described herein comprises (i) linoleic acid in an amount ranging from about 7g/Mcal to about 9g/Mcal, and (ii) zinc in an amount of about 50 mg/Mcal. In some embodiments, the foodstuff diet described herein comprises (i) linoleic acid in an amount ranging from 7g/Mcal to 9g/Mcal and (ii) zinc in an amount of about 50 mg/Mcal.

As used herein, an amount of zinc "about 50 mg/Mcal" includes zinc in an amount in the range of 45mg/Mcal to 55mg/Mcal, including the range of 48mg/Mcal to 52 mg/Mcal. In some embodiments, the zinc is present in an amount of 50 mg/Mcal.

In some embodiments, the diet or foodstuff comprises (i) linoleic acid in an amount in the range of from about 7g/Mcal to about 9g/Mcal and (ii) zinc in an amount in the range of from 40mg/Mcal to 60mg/Mcal, such as 45mg/Mcal to 55mg/Mcal, such as 50 mg/Mcal. In some embodiments, the diet or foodstuff comprises (i) linoleic acid in an amount in the range of 7 to 9g/Mcal and (ii) zinc in an amount in the range of 40 to 60mg/Mcal, such as 45 to 55mg/Mcal, such as 50 mg/Mcal.

In some preferred embodiments, the zinc source, i.e., the zinc-containing material, includes zinc sulfate, zinc oxide, organozinc complexes such as chelated zinc, zinc orotate, zinc picolinate, zinc gluconate, zinc acetate, or a combination of two or more thereof. In some preferred embodiments, the zinc source, i.e., the zinc-containing material, is selected from the group comprising zinc sulfate, zinc oxide, an organozinc complex, or a combination of two or more thereof.

In accordance with the present disclosure, when these substances are combined in a diet or foodstuff as described herein, the concentrations of linoleic acid and zinc can be used at any concentration, i.e., (i) any concentration of linoleic acid in the range of about 7g/Mcal to about 9g/Mcal, such as an amount of 7g/Mcal to 9g/Mcal, or 8g/Mcal, and (ii) any concentration of about 50mg/Mcal, such as any concentration of zinc in the range of about 40mg/Mcal to about 58mg/Mcal, such as any concentration of zinc in the range of about 45mg/Mcal to about 55mg/Mcal, such as any concentration of zinc in the range of about 48mg/Mcal to about 52mg/Mcal, such as any concentration of zinc in the range of 40mg/Mcal to 58mg/Mcal, such as any concentration of zinc in the range of 45mg/Mcal to 55mg/Mcal, for example, any concentration of zinc in the range of 48mg/Mcal to 52 mg/Mcal. Such concentrations include about 8g/Mcal linoleic acid and about 50mg/Mcal zinc. Such concentrations also include 8g/Mcal linoleic acid and 50mg/Mcal zinc.

Since zinc and linoleic acid are ubiquitous in food products, it will generally be desirable in accordance with the present disclosure to determine the concentration of each of zinc and linoleic acid present in the ingredients of a diet or foodstuff, and then add a sufficient amount to bring the total concentration of each of zinc and linoleic acid to the desired level. For example, a desired amount of linoleic acid or linoleic acid containing material may be added to a conventional pet diet or foodstuff that already contains linoleic acid, but in an amount that is less than the desired amount. By way of still further example, a desired amount of one or more zinc-containing materials can be added to a conventional pet diet or foodstuff that already contains zinc, but in an amount less than the desired amount.

To measure the linoleic acid content of a food composition, one skilled in the art can refer to any well-known technique. For example, a method based on specification NF EN ISO 5508/5509 via gas chromatography, which can be used on dry or wet diets.

To measure the zinc content of a foodstuff composition, the skilled person may refer to any well-known technique. For example, a method based on specification NF EN ISO 6869 via atomic absorption spectroscopy or ICP (for wet diets) may be used.

As already detailed previously in this disclosure, the diet or foodstuff described herein is most preferably composed of a comprehensive and balanced food product (e.g., as described by the American National Research Council (1985), Nutritional Requirements for dogs, National Academy of sciences publishers (National Academy Press), washington, or the American Feed Control Officials Association (Association of American Feed Control Officials), official publications 1996, the disclosure of each of which is incorporated herein by reference). I.e., the concentration levels of zinc and linoleic acid specified herein, are preferably used with high quality commercial food products. As used herein, "high-quality commercial food" refers to a diet manufactured to produce a protein digestibility of 80% or more, for example, as recommended by the national research council for dogs, above. Similar high nutritional standards will apply to other animals and humans.

The diet or foodstuff as described herein consists of a non-human animal diet or foodstuff, which is a wet or dry composition (food). Wet food products generally describe food products sold in canned form having a moisture content of from 70% to 90%. Dry food products generally describe food products having a similar composition, but a moisture content of 5% to 15%, and are therefore presented as small biscuit-like kibbles (small biscuit-kibbles).

In a preferred embodiment, the diet or foodstuff described herein consists of a dog diet or foodstuff.

The Diet or foodstuff may be prepared according to any method known in the art, for example, the Waltham Book of Dog and Cat Nutrition (Waltham Book of Dog and Cat Nutrition), edited by ATB Edney, chapter a. rainbird, pages 57 to 74, entitled "Balanced Diet (a Balanced Diet"), pergammon Press, oxford, the disclosure of which is incorporated herein by reference. The concentrations of zinc and linoleic acid to be added to the diet/feed are calculated based on the energy content of the diet/feed and any other nutrients that the animal may consume.

The present specification includes methods for preparing the diets or foodstuffs detailed herein. The method of manufacturing the foodstuff may be performed according to any method known in the art.

The diet or foodstuff may be made by mixing together and kneading a plurality of ingredients to produce a unified dough or meat emulsion (meat emulsion) that may be cooked. This also applies to liquids in which the ingredients are mixed well prior to the retort step in the package. The process of creating embodiments of the dry pet food is typically accomplished by baking and/or extrusion. The dough is typically fed into a machine called an expander and/or extruder that uses pressurized steam or water to cook the ingredients. While the dough is inside the extruder, it is under extreme pressure and at elevated temperature. The dough is then pushed through a die (having a hole of a particular size and shape) and then cut with a knife. The expanded dough pieces are passed through a dryer as a kibble to reduce the moisture to a defined target to ensure that the food product is stable prior to consumption. The kibble can then be sprayed with fat, oil, minerals, vitamins, natural extract mixture, palatant, and optionally sealed in a package.

The composition may be in the form of a powder or crumb, including a white powder or a solid form. It is useful to add the powder to the animal's main diet. Other forms include solid particles, granules, tablets or liquids.

The foodstuff diet herein is preferably packaged. In this way, the consumer is able to identify the ingredients in the food product from the packaging and confirm that it is appropriate for the particular pet in question. The packaging may be metal, plastic, paper or card (card).

The composition in the form of a diet or foodstuff may comprise any product consumed in the diet by the pet. Accordingly, the present disclosure encompasses standard food products as well as pet food snacks (e.g., snack bars, cookies, and desserts). The diet or foodstuff herein is preferably a cooked product. It may be incorporated into meat or animal derived materials such as those derived from beef, chicken, turkey, pork, duck, kangaroo, lamb or fish, blood plasma, bone marrow, feather (e.g., feather hydrolysates such as poultry feather hydrolysate), and the like. The product may optionally be meat free (preferably including meat substitutes such as soy, zein (corn gluten) or soy hydrolysate) to provide a protein source. The product may comprise other protein sources such as vegetable proteins (wheat gluten, pea protein) or soy protein concentrates or hydrolysates, milk proteins, gluten (gluten), etc.). The product may also comprise a starch source, such as one or more grains (e.g., wheat, corn, rice, oats, barley, etc.), or carbohydrates from other sources (e.g., potatoes), or no starch. The product may comprise fibres, such as chicory (chicory), beet pulp (sugar beet pulp) etc. and/or ingredients such as inulin (inulin), fructo-oligosaccharides (fructicosaccharide), probiotics (probiotics), most preferably the combined ingredients of the diet or foodstuff may provide all recommended vitamins and minerals (a comprehensive and balanced food) for the specific animal in question, as described for example in the 1985 american national research council, the nutritional requirements for dogs, the american national academy of sciences press, the washington district or the american association for feed control officials (official publication 1996).

As already detailed elsewhere herein, the dietary diets of the present disclosure improve skin quality, particularly by reducing trans-dermal water loss (TEWL), for example in pet animals such as dogs.

Thus, in some preferred embodiments, the diet or foodstuff is suitable for feeding healthy dogs.

In some preferred embodiments, the diet or foodstuff is used to maintain or improve the skin quality of healthy dogs.

As used herein, the term "healthy pet animal" refers to a pet animal that is not affected by any skin disease or disorder. For example, the healthy pet animal may be a healthy dog. As used herein, a "healthy dog" consists of a dog that is not suffering from any skin disease or disorder. Skin diseases or conditions include dermatitis, psoriasis, atopic dermatitis, skin forms of food allergies, pruritic diseases, bacterial folliculitis, and furunculosis. Dermatitis includes allergic dermatitis (e.g., flea bite allergic dermatitis or poor skin food reaction), atopic dermatitis (e.g., environmental atopic dermatitis), pyoderma (bacterial infection), mange (demodex and scabies), and immune or autoimmune dermatitis.

The present disclosure also relates to a method for maintaining or improving the skin quality of a healthy pet animal comprising the step of feeding the healthy pet animal with a diet or foodstuff as described herein.

In some embodiments, the method comprises the step of feeding a healthy pet animal with a diet or foodstuff comprising linoleic acid in an amount ranging from about 7g/Mcal to about 9 g/Mcal. In some embodiments, the method comprises the step of feeding a healthy pet animal with a diet or foodstuff comprising linoleic acid in an amount in the range of 7g/Mcal to 9 g/Mcal. In some embodiments, the diet or foodstuff comprises linoleic acid in an amount of about 8g/Mcal, for example 8 g/Mcal.

In some embodiments, the diet or foodstuff further comprises zinc in an amount in the range of about 50mg/Mcal, for example about 40mg/Mcal to about 60mg/Mcal, for example about 45mg/Mcal to about 55mg/Mcal, for example 40mg/Mcal to 60mg/Mcal, for example 45mg/Mcal to 55mg/Mcal, for example 50 mg/Mcal.

In a preferred embodiment of the feeding method, the diet or foodstuff described herein is fed daily to a healthy pet animal, such as a healthy pet dog. In other embodiments, a healthy pet animal, such as a healthy pet dog, is fed the diet or foodstuff described herein every three of two, four days.

The present disclosure also relates to a diet or foodstuff comprising linoleic acid in an amount in the range of from about 7g/Mcal to about 9g/Mcal, for example in an amount of from 7g/Mcal to 9g/Mcal, for example about 8g/Mcal, for use in maintaining or improving skin quality in a pet animal. In some embodiments, the diet or foodstuff comprises linoleic acid in an amount of 8 g/Mcal.

In some embodiments, the diet or foodstuff comprises (i) linoleic acid in an amount ranging from about 7g/Mcal to about 9g/Mcal, and (ii) zinc in an amount ranging from about 40mg/Mcal to about 60 mg/Mcal; for maintaining or improving skin quality in a pet animal. In some embodiments, the diet or foodstuff comprises (i) linoleic acid in an amount ranging from 7g/Mcal to 9 g/Mcal; and (ii) zinc in an amount in the range of 40mg/Mcal to 60 mg/Mcal; for maintaining or improving skin quality in a pet animal.

In some embodiments, the diet or foodstuff further comprises zinc in an amount of about 50 mg/Mcal. In some embodiments, the diet or foodstuff further comprises zinc in an amount of 50 mg/Mcal.

In some embodiments, the diet or foodstuff is a dog diet or foodstuff.

The experimental results contained in the examples show that the diet or foodstuff described herein improves the skin quality and specifies that the improvement in skin quality results in beneficial effects on body hair, for example on the shine, smoothness and softness of body hair of healthy pet animals, such as healthy dogs.

The inventors have also determined that the improvement in skin quality provided by the diet or foodstuff described herein may also be beneficial to pet animals suffering from skin diseases or disorders. Indeed, skin diseases or conditions cause changes in the skin barrier, for example, leading to changes in transdermal water loss, which can be corrected by feeding the diet or foodstuff to unhealthy pet animals.

Thus, according to the second aspect of the present disclosure, the diet or foodstuff described herein may be used for therapeutic purposes in treating a pet animal suffering from a skin disease or disorder.

According to the second aspect described above, the present disclosure relates to a diet or foodstuff comprising linoleic acid in an amount in the range of from about 7g/Mcal to about 9g/Mcal, for example 7g/Mcal to 9g/Mcal, for use in treating a pet animal afflicted with a skin disease or disorder.

In some embodiments, the present disclosure relates to a method of treating a pet animal afflicted with a skin disease or disorder, the method comprising feeding the pet animal a diet or foodstuff comprising linoleic acid in an amount ranging from about 7g/Mcal to about 9g/Mcal, e.g., 7g/Mcal to 9 g/Mcal.

According to this second aspect, the present disclosure also relates to the use of linoleic acid in an amount ranging from about 7g/Mcal to about 9g/Mcal, for example from 7g/Mcal to 9g/Mcal, for the preparation of a pharmaceutical diet or foodstuff for the treatment of dogs suffering from diseases or dermatological conditions causing skin.

Still according to this second aspect, the present disclosure also relates to a method for treating a pet animal suffering from a skin disease or disorder, the method comprising feeding the pet animal a diet or foodstuff comprising linoleic acid in an amount in the range of from about 7g/Mcal to about 9g/Mcal, for example 7g/Mcal to 9 g/Mcal.

According to this second aspect, the skin disease or condition is preferably selected from the group comprising dermatitis, psoriasis, atopic dermatitis, skin forms of food allergies, pruritic diseases (pruritic disease), bacterial folliculitis, and furunculosis. Dermatitis includes allergic dermatitis (e.g., flea bite allergic dermatitis or poor skin food reaction), atopic dermatitis (e.g., environmental atopic dermatitis), pyoderma (bacterial infection), mange (demodex and scabies), and immune or autoimmune dermatitis.

For clarity, in a preferred embodiment of the second aspect of the present disclosure, the food or diet for treating a pet animal suffering from a skin disease or disorder may be any embodiment of the diet or foodstuff described elsewhere in the present disclosure when used to improve the skin quality of a healthy pet animal. Unless otherwise indicated, various embodiments of the dietary diets for non-therapeutic purposes described elsewhere herein are expressly incorporated into possible embodiments of the diet or diet as a purpose for treating a pet animal suffering from a skin disease or disorder.

The disclosure is further illustrated by, but is not in any way limited to, the following examples.

Examples

Example 1: specific combinations of linoleic acid and zinc improve skin characteristics in companion animals

Objects of the embodiments

The aim was to study the effect of diets containing different concentrations of zinc and linoleic acid on the properties of labrador black skin. The diet contains more zinc and linoleic acid than the usual minimum standards, as recommended by NRC (national research council nutritional requirements for cats and dogs), FEDIAF (european pet food industry association of european animals sources of animals), and AAFCO (american feed control official association), but below previously shown levels that improve skin and hair.

A. Materials and methods

Four diets were fed during the study. All 34 black labrador were fed a benchmark diet with recommended minimum levels of zinc (21mg/1000kcal) and linoleic acid (3.8g/1000kcal) for the 12 weeks prior to the start of the trial. The dogs were then randomized into 4 groups of 8 dogs each. One group continued on the baseline diet (diet a) while the other 3 groups were transferred to diet B, diet C and diet D, with zinc and linoleic acid concentrations as shown in table 1 below.

Table 1: test diets

Diet	Zinc (mg/1000kcal)	Linoleic acid (g/1000kcal)
			A	21	3.8
B	50	6
			C	50	8
D	50	10

Some other components of each diet are also described in table 2 below.

Table 2: other components of the diet

	Diet A	Diet B	Diet C	Diet D
					Ash content% (w/w)	6.2	6.9	6.9	6.5
Cellulose% (w/w)	1.8	1.6	1.5	1.6
					Fat% (w/w)	13.3	14.4	13.8	13.2
Protein% (w/w)	25.4	24.8	24.4	24.2
					Arachidonic acid (g/Mcal)	0.129	0.129	0.143	0.163
EPA/DHA(g/Mcal)	0.15	0.11	0.12	0.15

To assess skin characteristics, trans-dermal water loss (TEWL) was measured at weeks 0, 6 and 12 after initiation of feeding the dogs with each particular diet/diet.

TEWL measurements were taken at the end of the pre-feeding period (pre-feeding period) and after weeks 4, 8 and 12 of the subsequent differential feeding period (differential feeding period). Five separate measurements were made for each dog at each time point. Dogs were evaluated in a quiet, non-ventilated (draft free) room with uniform environmental conditions. Measurements were made using a Cutomer MPA580 equipped with a Tewameter Triple TM 330T (3 probes measuring TEWL simultaneously), which was placed 1-2 inches on one side of the lumbar spine (lumbar spine), with the hairs first separated to ensure that the probes maintained good contact with the skin. The lumbar region of the animal provides a good surface for the orientation of the 3 probes.

Dogs were acclimated to rest for 1 minute prior to study initiation to improve stability of readings. TEWL was measured twice for all dogs at each time point, and for each measurement, three probes were used to record for up to 90 seconds per second. According to the probe manufacturer's literature, the expected curve over 90 seconds will increase to a plateau, which is the measured TEWL value. For analysis, the first 30 seconds of recording were deleted and the remaining (longest) 60 seconds were used as duplicates. From a statistical perspective, TEWL modeling was performed for weeks (Week), Diet (die), and weeks with fixed effect through Diet interaction, with randomly acting structures of probes nested in repeats, in weeks (Week), and in dogs (Dog). The same comparison as described for the other models applies.

B. Results

The estimated tewl (estimated tewl) for each diet at weeks 0, 6 and 12 is shown below, with a Confidence Interval (CI) of 98.75%. The results are shown in Table 3 below.

Table 3: estimated TEWL

The differences between diets varied from week 0 and between weeks for each diet are shown in table 4 and figure 1 below.

TABLE 4 TEWL differences between diets

Response to	Comparison of	Estimating	98.75％CI	p-value
					TEWL	(diet B: week 6-week 0) - (diet A: week 6-week 0)	-1.29	(-9.85,7.28)	0.9998
TEWL	(diet B: week 12-week 0) - (diet A: week 12-week 0)	-2.24	(-10.80,6.33)	0.9889
					TEWL	(diet C: week 6-week 0) - (diet A: week 6-week 0)	-8.38	(-16.81,0.06)	0.0137
TEWL	(diet C: week 12-week 0) - (diet A: week 12-week 0)	-4.82	(-13.26,3.61)	0.4870
					TEWL	(diet D: week 6-week 0) - (diet A: week 6-week 0)	-1.65	(-9.80,6.51)	0.9981
TEWL	(diet D: week 12-week 0) - (diet A: week 12-week 0)	0.70	(-7.32,8.71)	1.0000
					TEWL	Diet A, 6-0 weeks	0.26	(-5.40,5.93)	1.0000
TEWL	Diet A, week 12-week 0	-2.69	(-8.36,2.97)	0.7216
					TEWL	Diet A week12-week 6	-2.96	(-8.62,2.71)	0.6100
TEWL	Diet B, 6-0 weeks	-1.02	(-7.45,5.40)	0.9997
					TEWL	Diet B, week 12-week 0	-4.93	(-11.36,1.49)	0.1293
TEWL	Diet B, 12-6 weeks	-3.91	(-10.33,2.52)	0.3996
					TEWL	Diet C6-week 0	-8.11	(-14.36,-1.86)	0.0002
TEWL	Diet C, week 12-week 0	-7.52	(-13.77,-1.27)	0.0010
					TEWL	Diet C, 12-6 weeks	0.59	(-5.83,7.02)	1.0000
TEWL	Diet D6-week 0	-1.38	(-7.24,4.48)	0.9944
					TEWL	Diet D, week 12-week 0	-2.00	(-7.66,3.67)	0.9326
TEWL	Diet D, week 12-week 6	-0.61	(-6.48,5.25)	1.0000

The results show a significant reduction in the transdermal water loss (TEWL) when fed to animals on diet C to a high degree. All other diets tested, in particular diet B (6mg/Mcal linoleic acid and 50mg/Mcal zinc) and D (10mg/Mcal linoleic acid and 50mg/Mcal zinc) did not indeed cause significant changes in the trans-dermal water loss (TEWL).

***

Although the presently disclosed subject matter and its advantages have been described in detail, it should be understood that various changes, substitutions and alterations can be made herein without departing from the spirit and scope of the invention as defined by the appended claims. Moreover, the scope of the present application is not intended to be limited to the particular embodiments of the process, machine, manufacture, composition of matter, means, methods and steps described in the specification. As one of ordinary skill in the art will readily appreciate from the disclosure of the disclosed subject matter, processes, machines, manufacture, compositions of matter, means, methods, or steps, presently existing or later to be developed that perform substantially the same function or achieve substantially the same result as the corresponding embodiments described herein may be utilized according to the disclosed subject matter. Accordingly, the appended claims are intended to include within their scope such processes, machines, manufacture, compositions of matter, means, methods, or steps.

Patents, patent applications, publications, product descriptions, and protocols are cited in this application, the disclosures of which are incorporated herein by reference in their entirety for all purposes.