阅读说明：本技术 增加漆黄素含量的漆树提取物的制备方法及包含其的用于转移抑制的抗癌剂组合物 (Preparation method of Rhus verniciflua Stokes extract with increased fisetin content, and anticancer agent composition for metastasis inhibition comprising the same ) 是由 朴相再 金京姬 柳尧喜 于 2018-10-12 设计创作，主要内容包括：本发明涉及增加漆黄素含量的漆树提取物的制备方法及包含其的用于转移抑制的抗癌剂组合物,更详细地,涉及如下增加漆黄素含量的漆树提取物的制备方法和以通过上述方法制备的增加漆黄素含量的漆树提取物作为主要成分的用于预防或改善癌症的保健功能食品用组合物和用于抑制癌症转移的抗癌用药学组合物：通过将选自由铂、铬、镍、硅、铜及这些金属的氧化物组成的组中的一种以上的催化剂添加至漆树提取液或漆树提取浓缩液并进行反应,来使上述漆树中含有的黄颜木素转化为漆黄素。由此,本发明提供能够通过增加天然提取物的主要功能成分来制备提供更为改善的抗氧化活性及抗癌活性的提取物的非常安全且有效的方法及抗癌剂组合物,可以通过制备为天然物抗癌剂、功能性食品等来为国民的健康做出贡献。(The present invention relates to a method for preparing a sumac extract having an increased fisetin content and an anticancer agent composition for metastasis inhibition comprising the same, and more particularly, to a method for preparing a sumac extract having an increased fisetin content, and a health functional food composition for preventing or improving cancer and an anticancer pharmaceutical composition for inhibiting metastasis of cancer, which contain the fisetin-increased fisetin extract prepared by the above method as a main component, as follows: the fustin contained in the sumac is converted into fisetin by adding one or more catalysts selected from the group consisting of platinum, chromium, nickel, silicon, copper, and oxides of these metals to a sumac extract or a sumac extract concentrate and reacting the same. Accordingly, the present invention provides a very safe and effective method and anticancer agent composition capable of preparing an extract providing more improved antioxidant activity and anticancer activity by increasing the main functional components of a natural extract, and can contribute to the health of the nation by preparing a natural anticancer agent, a functional food, and the like.)

1. A method for preparing a Rhus verniciflua Stokes extract with increased fisetin content, characterized in that more than one catalyst selected from the group consisting of platinum, chromium, nickel, silicon, copper and oxides of these metals is added to a Rhus verniciflua Stokes extract or a Rhus verniciflua Stokes extract concentrate and reacted to convert fustin contained in the above Rhus verniciflua Stokes into fisetin.

2. The method of claim 1, wherein the metal or metal oxide catalyst is added to the sumac extract or the sumac extract concentrate by itself or by impregnation with a carrier.

3. The method for producing a sumac extract with an increased fisetin content according to claim 1, characterized in that the catalyst is added in a step selected from the group consisting of during the extraction step of a sumac extract, after the completion of the extraction step, and after the concentration of the extract.

4. The method for producing a sumac extract having an increased fisetin content according to claim 1, characterized by adding a step of adding an inert gas to the sumac extract or the sumac extract concentrate to foam the same during the catalytic reaction with the catalyst.

5. A Rhus verniciflua Stokes extract having an increased content of fisetin, which is produced by the production method according to any one of claims 1 to 4.

6. A health functional food composition for preventing or ameliorating cancer, comprising the Rhus verniciflua Stokes extract of claim 5 as an active ingredient.

7. An anticancer pharmaceutical composition for inhibiting cancer metastasis, which comprises the Rhus verniciflua Stokes extract of claim 5 as an active ingredient.

8. The anticancer pharmaceutical composition for inhibiting cancer metastasis according to claim 7, wherein the cancer whose metastasis is inhibited is a malignant tumor selected from the group consisting of stomach cancer, liver cancer, large intestine cancer, lung cancer, breast cancer, rectal cancer, blood cancer and pancreatic cancer.

Technical Field

The present invention relates to a method for obtaining the following Rhus verniciflua Stokes extract and an anticancer agent composition for inhibiting metastasis containing the same: in the preparation of the Rhus verniciflua Stokes extract, the fustin, one of the main components of Rhus verniciflua Stokes, is converted into fisetin, so that fisetin has stronger anticancer activity.

Background

The lacquer is a plant belonging to the family of the Anacardiaceae, which is divided into a mountain lacquer and a raw lacquer, and the lacquer liquid is a slurry flowing out after the trees are cut open. The lacquer contains urushiol or flavonoid compounds, has an anticancer effect with a remarkable antioxidant effect, or has effects of preventing aging, removing toxins and removing liver toxins, reducing blood stasis in vivo and warming the body, or improving digestive function to chronic gastrointestinal diseases, etc., and particularly, is known to have a more remarkable effect than that of lacca.

The lacquer tree native to korea, japan, china, etc. is known as rhussvericifua STOKES. Paint has many pharmacological effects, but contains urushiol, which is known as an allergy-inducing substance, and therefore, when taken directly or contacted with leaves, serum, or the like, serious allergic symptoms such as rash, pruritus, or the like are often induced. Therefore, it should be used with great care. Recently, although a technique for removing a urushiol component has been developed, it has been found that there is a problem that the efficacy is lowered in the case of removing a urushiol component.

On the other hand, the absorption rate of useful components contained in sumac is very low, although sumac is used as a material for foods and medicines. Therefore, there is a need to develop a method for improving the absorption rate of useful components of the sumac extract.

Generally, sumac extract is known to contain fustin, fisetin, sulfopartrin, butein, and the like as flavonoids, and the content of fustin is usually very high. However, the results of the study showed that the anticancer activity of fustin was very low, while that of fisetin was the highest. Therefore, in order to increase the anticancer activity of the sumac extract, the content of fisetin needs to be increased. Generally, fustin can be converted into fisetin by using an organic synthesis method, but this process requires a variety of chemicals and is not highly likely to be converted, and therefore, it is an important subject to find a method capable of conversion without using an organic solvent such as acetone or hexane, and the like, for obtaining a fisetin extract having high anticancer activity.

The catalyst is not included in the actual contents although it is involved in the reaction, and is used for reducing the reaction energy as a general method capable of controlling the chemical reaction. Accordingly, the present inventors have made extensive studies to find a method for increasing the content of fisetin in sumac, and focused on the conversion of fustin into fisetin using such a catalyst, and have developed a method using a relevant catalyst to prepare a fisetin extract having a high fisetin content, and have confirmed that the extract has an activity as an anticancer agent for inhibiting the metastasis of cancer cells, thereby completing the present invention.

Disclosure of Invention

Technical problem

The present invention is directed to providing a method for preparing a sumac extract having more enhanced anticancer activity by increasing the content of fisetin in the sumac extract by developing a method for converting fustin, which is a water-soluble flavonoid contained in the sumac extract, into fisetin having more excellent anticancer activity.

Another object of the present invention is to provide an anticancer pharmaceutical composition or health functional food for inhibiting cancer metastasis, which provides superior physiological activity and has significantly improved anticancer activity by using the above sumac extract having an increased fisetin content.

Technical scheme

In order to achieve the above object, the present invention provides a method for preparing a sumac extract with increased fisetin content, comprising: the fustin contained in the sumac is converted into fisetin by adding one or more catalysts selected from the group consisting of platinum, chromium, nickel, silicon, copper, and oxides of these metals to a sumac extract or a sumac extract concentrate and reacting the same.

Also, the present invention provides a sumac extract increased in fisetin content, prepared by the above preparation method.

The present invention also provides a functional food composition for health care and an anticancer pharmaceutical composition for inhibiting cancer metastasis, which contains the above sumac extract as a main ingredient.

The present invention will be described in detail below.

The invention provides a preparation method of a sumac extract for increasing the content of fisetin, which comprises the following steps: the fustin contained in the sumac is converted into fisetin by adding one or more catalysts selected from the group consisting of platinum, chromium, nickel, silicon, copper, and oxides of these metals to a sumac extract or a sumac extract concentrate and reacting the same.

In the method for producing a sumac extract having an increased fisetin content of the present invention, the catalyst as the metal or metal oxide is preferably added to the sumac extract or the concentrated sumac extract by itself or by impregnating the catalyst with a carrier, and the catalyst is preferably added in a step selected from the group consisting of during the extraction step of the sumac extract, after the completion of the extraction step, and after the concentration of the extract.

In the method for producing a sumac extract having an increased fisetin content according to the present invention, it is preferable to add a step of removing oxygen to suppress oxidation of the catalyst during the catalytic reaction by the catalyst, and adding an inert gas to the sumac extract or the sumac extract concentrate to foam the same.

Also, the present invention provides a sumac extract increased in fisetin content, prepared by the above preparation method.

The present invention provides a functional food composition for health care and for preventing or improving cancer and a pharmaceutical composition for anticancer for inhibiting cancer metastasis, which contain the above Rhus verniciflua Stokes extract as a main ingredient.

In the pharmaceutical composition for anticancer of the present invention, preferably, the cancer whose metastasis is inhibited is a malignant tumor selected from the group consisting of gastric cancer, liver cancer, colorectal cancer, lung cancer, breast cancer, rectal cancer, blood cancer, and pancreatic cancer.

Generally, it is known that the content of fustin contained in a sumac extract is about 5% to 20% by weight and the content of fisetin is about 0% to 5% by weight, which is a known method in general organic synthesis, but there is no method for converting fustin into fisetin while maintaining the antioxidant activity of flavonoids without substantially affecting other components. Generally, oxidation refers to a phenomenon in which a target component is oxidized by contact with oxygen, and although a natural extract has antioxidant activity, contact with excessive oxygen causes a decrease in antioxidant ability. The process of converting fustin to fisetin is not a general oxidation process, but may be a reduction process with the addition of hydrogen.

The present invention, by using a catalyst, not only does not decrease but also increases the antioxidant capacity of the extract, ensures the conditions for converting fustin, which cannot be converted by the conventional method, into fisetin, and confirms that it can be used for the development of an anticancer agent composition having an effect of inhibiting cancer metastasis.

ADVANTAGEOUS EFFECTS OF INVENTION

The present invention provides a very safe and effective method and anticancer agent composition capable of preparing an extract providing more improved antioxidant activity and anticancer activity by increasing the main functional components of a natural extract, and can contribute to the health of the nation by preparing a natural anticancer agent, a functional food, and the like.

Drawings

FIG. 1 is the chemical structural formula of fustin.

FIG. 2 shows the chemical structure of fisetin.

Fig. 3 is a graph comparing the efficacy of the sumac extract prepared according to the example of the present invention in inhibiting cancer cell metastasis.

Detailed Description

The present invention can employ a method of preparing a sumac extract, treating the prepared extract or concentrating the extract, so that the catalyst added for the reaction can be removed by filtration in the subsequent filtration step, and the reaction solution can be obtained as an extract with enhanced physiological activity.

It is known that there is no precedent for using a catalyst for such a purpose, which is aimed at a sumac extract. The present invention has been developed from such a viewpoint by a new concept, and relates to a novel process and a novel composition which are not easily developed by even a researcher who is specialized in organic synthesis.

The present invention begins with the preparation of a sumac extract. The catalyst used in the step of converting the fustin, which is the main step of the present invention, into fisetin may be one or more of platinum, copper, chromium, silicon, and nickel, and preferably one or more of platinum and chromium. In the case of these metals, forms of pure metals, oxides, nitrogen oxides, and the like can be employed, and these have effects of promoting the progress of the reaction itself, and only to a certain extent vary. Usually, pure metals are the best, followed by oxides, oxynitrides in that order. For the efficiency of the reaction, the following general catalyst use method may be employed: in order to increase the reaction surface area and thereby more efficiently perform the reaction over a wide area, these catalysts have been prepared by coating a porous material such as activated carbon or zirconia on the surface of pores of a carrier, and then adding the catalyst to the carrier to increase the reaction surface area by several hundred times, thereby minimizing the amount of the catalyst used and maximizing the reaction. The catalyst can be prepared by a conventional method, that is, a method in which a metal or an oxide thereof is dissolved in a solvent, the carrier is impregnated with the solution, and then dried to support the metal component on the carrier, and the metal component is added and used as a catalyst.

The Rhus verniciflua Stokes extract can be prepared by extracting with water or diluted ethanol at 50-80 deg.C in the case of ethanol, or 60-100 deg.C in the case of water, but the extraction temperature can be higher than the applicable range of the system. The amount of the solvent used is usually about 4 to 10 times the weight of the charged sumac, and this is determined economically in consideration of efficiency, and is irrelevant to whether extraction is possible or not. The extraction time is usually selected in the range of 4 hours to 10 hours, in view of maximizing the extraction efficiency and considering the economy. If the amount of the solvent is small in the first extraction, the number of times of extraction may be up to the second or third extraction.

The point of time of catalyst input may be as follows: adding the extract simultaneously with extraction, or filtering the extract alone and then adding the extract, or concentrating the extract and then adding the concentrate.

If added simultaneously with the extraction, the water-soluble fustin is extracted from the extract and at the same time converted into fisetin by the catalyst, but this forms a precipitate and a partial loss occurs in the case of being caught in a filter during the filtration process. But has the advantage of facilitating both extraction and transformation. The fisetin forming the precipitate sticks to the filter during the filtration, but in order to increase the recovery rate of fisetin, the fisetin sticking to the filter may be dissolved and recovered using a solvent such as alcohol, which can dissolve the fisetin precipitate.

In the case where the catalyst is added to the extract liquid after the extract liquid is separately collected, the conversion can be made easier, and there is an advantage that the formation of a precipitate of fisetin having low solubility can be adjusted by making the volume of the solvent large, thereby adjusting the size of the fisetin precipitate. However, in the case of the concentrated solution, fisetin precipitates are often formed during the concentration process, and there is a difference in the size of fisetin crystals, which is difficult to adjust as compared with the extract solution.

The subsequent conversion process in the concentration step is to form a precipitate of fisetin in a trace amount when the sumac extract is concentrated, but the water-soluble fustin remains dissolved and is easily converted into fisetin by the action of the catalyst. Also, the catalytic reaction for converting fustin into fisetin continues to form a precipitate and is separated from the solution as a result of the reaction that the solubility of generated fisetin in an aqueous solution is low, and thus the balance of the reaction is shifted to a positive direction in which fustin is actively converted into fisetin, thereby maximizing the reaction efficiency and greatly improving the conversion rate. For this purpose, the following method can also be adopted: the formation of crystals of fisetin is induced by circulating a part of the contents to the outside and cooling, and the concentration of fisetin in the reaction solution is reduced by refluxing, so that the balance of conversion of fustin to fisetin is actively shifted to fisetin. Further, since foreign matter is removed by filtration in advance, a separate filtration step is not required as in the case of using a solvent for the extraction liquid. However, since the precipitation of fisetin occurs naturally during the concentration process, the dissolution/crystallization formation process is also followed in the case where the size of the precipitate needs to be adjusted.

The amount of the catalyst to be added can be at least 0.1% (w/w) and at most 30% (w/w) based on the amount of the solid component in the extract or the concentrate, and such a limit is used merely in consideration of economical efficiency and has no influence on the progress of the usual reaction. That is, the catalyst does not disappear during the reaction, although it is involved in the reaction, and therefore, even a trace amount of the catalyst can catalyze the reaction. However, a very small amount of the catalyst causes a problem of low conversion rate in terms of a long time required for the reduction of the probability of molecular collision associated with the reaction, and if the catalyst is added in an excessively high proportion, the reaction time can be shortened, but the target solution is a complex of the extract, and another unexpected additional reaction may be caused. Excessive addition of the catalyst does not continuously increase the conversion rate into fisetin, but rather causes the disappearance of fisetin produced, and therefore the final fisetin content tends to be low.

Therefore, in the present invention, when the fusel pigment is converted into fisetin by adding a catalyst, the content of fusel pigment is usually 3% (w/w) or less and the fisetin content is maintained in the range of 5% to 20% (w/w) in the final sumac extract, which is greatly influenced by the concentration of the fusel pigment and fisetin contained in the raw sumac. When the age of the raw material sumac is more than 10 years, the total content of flavonoids is remarkably increased, so that the result that the content of fisetin is increased after the conversion into fisetin by adding a catalyst can be obtained.

In the present invention, the sumac extract converted into fisetin exhibits an increase in antioxidant activity of about 20% to 35% as compared with the sumac extract before conversion, and this is expected to be data showing improved physiological activity.

In general, the higher the temperature of the reaction after the addition of the catalyst, the faster the reaction rate, and therefore, the reaction is easy in the range of 60 ℃ to 100 ℃, and for the faster reaction, a temperature of 100 ℃ or higher may be used, but an excessively high temperature may increase the possibility of causing an unexpected reaction due to excessively high reactivity. Further, when the temperature is lower than 60 ℃, there is a disadvantage that the reaction time is prolonged due to the decrease in reactivity, and the reaction proceeds without any problem.

The reaction time varies depending on the temperature and the amount of the catalyst, and when the amount of the catalyst is added in a proportion of 1% (w/w) to 10% (w/w) to the fixed component, the reaction time is preferably 2 to 6 hours at a reaction temperature of 90 ℃.

As one of the reaction conditions, oxygen present during the reaction can be generally judged as inhibiting the reaction. The reason for this is that the reaction for converting the fustin into fisetin is a dehydrogenation reaction, and if oxygen is present, the oxidation reaction of the added catalyst is promoted, thereby reducing the efficiency of the reaction, and as a result, there is a possibility that the yield of fisetin may be reduced. Therefore, it is preferable to remove oxygen by a method of purging with an inert gas such as nitrogen or a method of evacuating.

After the catalytic reaction is finished, the catalyst is cooled, and in the process, the originally dissolved fisetin crystals containing tiny precipitates of fisetin gradually grow and the size of fisetin gradually becomes larger. Therefore, after recovering such fisetin, in order to remove the catalyst, the reaction solution may be concentrated to a solid content concentration of 50% (w/v) or more, pure alcohol may be added thereto, and the solution may be heated to dissolve all fisetin, and then the catalyst may be removed by filtration. Thereafter, when the filtrate is concentrated to remove the solvent, the "sumac extract increased in fisetin" from which the catalyst is removed can be obtained.

The obtained Rhus verniciflua Stokes extract with increased fisetin can be used as anticancer agent for inhibiting cancer metastasis, functional food, etc.

The sumac extract enriched with fisetin prepared in the present invention may be mixed with saccharides, lecithin, cellulose, and the like, which are generally used in the preparation of foods and medicines, to prepare a composition containing a plurality of formulations of the sumac extract powder, in a liquid or powder form.

In another aspect, the present invention provides an anticancer agent composition comprising a sumac extract having an increased fisetin content. The term "comprising" as used herein means that an amount of "fisetin" is added to the anticancer agent composition of the present invention to such an extent that the composition can exert its efficacy as an anticancer agent.

The anticancer composition of the present invention may comprise a pharmaceutically effective amount of Rhus verniciflua Stokes extract alone, and may further comprise one or more pharmaceutically acceptable carriers, excipients or diluents. The "pharmaceutically effective amount" refers to an amount sufficient for preventing, ameliorating and treating symptoms of cancer.

The pharmaceutically effective amount of the Rhus verniciflua Stokes extract of the present invention is 0.5 mg/day/kg body weight to 100 mg/day/kg body weight, preferably 0.5 mg/day/kg body weight to 5 mg/day/kg body weight. However, the pharmaceutically effective amount may be suitably changed depending on the degree of the symptoms of cancer, the age, body weight, health state, sex, administration route and treatment time of the patient.

Also, the expression "pharmaceutically acceptable" as referred to above means physiologically acceptable and does not generally cause abnormal reactions such as gastrointestinal disorders, vertigo or the like when administered to a human body. Examples of the carrier, excipient and diluent include lactose, glucose, sucrose, sorbitol, mannitol, xylitol, erythritol, maltitol, starch, gum arabic, alginate, gelatin, calcium phosphate, calcium silicate, cellulose, methyl cellulose, polyvinylpyrrolidone, water, methyl hydroxybenzoate, propyl hydroxybenzoate, talc, magnesium stearate and mineral oil. Further, a filler, an anticoagulant, a lubricant, a wetting agent, a perfume, an emulsifier, a preservative, and the like may be contained.

Also, the compositions of the present invention may be formulated by methods well known in the art to provide rapid, sustained or delayed release of the active ingredient after administration to a mammal. The dosage form can be powder, granule, tablet, emulsion, syrup, spray, soft or hard gelatin capsule, sterile injectable solution, or sterile powder.

The anticancer composition of the present invention can be administered by various routes including oral, transdermal, subcutaneous, intravenous or intramuscular routes, and the dose of the active ingredient can be appropriately selected depending on various factors such as the route of administration, the age, sex, body weight of the patient, and the severity of the patient. The anticancer composition of the present invention may be administered concurrently with a known compound having an effect of preventing, ameliorating or treating a cancer symptom. Accordingly, the present invention can provide an agent for preventing and/or treating cancer symptoms, which comprises the above sumac extract as an active ingredient.

Furthermore, the anticancer composition of the present invention can provide an effect of alleviating cancer symptoms by excellent anticancer and antioxidant effects, and can be added to food for the purpose of preventing and improving cancer symptoms. Therefore, the composition of the present invention can be flexibly used as a food having a cancer symptom preventing and improving effect, for example, a main material, an auxiliary material, a food additive, a functional food or a beverage of a food.

In the present invention, the term "food" refers to a natural product or processed product containing one or more nutrients, and preferably refers to a state that can be directly eaten after undergoing any degree of processing steps. In the usual sense, all foods, food additives, functional foods and beverages are included.

Examples of foods to which the anticancer composition of the present invention can be added include various foods, beverages, chewing gums, teas, vitamin preparations, functional foods, and the like. Further, the food that can be used in the present invention includes special nutritional foods (for example, prepared milks, infant foods, etc.), meat processed products, fish products, tofu, jelly, noodles (for example, pulled noodles, etc.), bread, health supplementary foods, seasoned foods (for example, soy sauce, soybean paste, chili paste, mixed paste, etc.), sauces, cookies (for example, snacks), candies, chocolates, chewing gums, ice creams, dairy products (for example, fermented milk, cheese, etc.), other processed foods, kimchi, pickled foods (various pickles, etc.), beverages (for example, fruit beverages, vegetable beverages, soy milk, fermented beverages, etc.), natural seasonings (for example, pulled noodle soup, etc.), and the like, but is not limited thereto. The above-mentioned food, drink or food additive can be prepared by a usual preparation method.

The "functional food" is a food group to which added value is added by physical, biochemical and biological engineering means in foods in order to apply the function of the food and express a specific purpose, or a food designed and processed in order to sufficiently express the in vivo regulatory function related to the regulation of the defense rhythm of the living body and the prevention and recovery from diseases, etc. of a combination of foods, and specifically, may be a health functional food. The functional food may contain a food auxiliary additive acceptable in terms of food science, and may further contain a suitable carrier, excipient and diluent generally used in the preparation of functional foods.

The term "beverage" refers to a general term for drinking for quenching thirst or enjoying taste, and includes functional beverages. The other ingredients contained in the above beverage are not particularly limited except for the composition for preventing and improving cancer symptoms as described above contained as essential ingredients in the indicated proportions, and may contain additional ingredients such as various flavors or natural carbohydrates as in the ordinary beverage.

Further, the food containing the composition of the present invention may contain various nutrients, vitamins, minerals (electrolytes), flavors such as synthetic flavors and natural flavors, colorants and fillers (cheese, chocolate and the like), pectic acid and salts thereof, alginic acid and salts thereof, organic acids, protective colloid thickeners, pH adjusters, stabilizers, preservatives, glycerin, alcohol, carbonating agents for carbonated beverages, and the like, and these components may be used alone or in combination.

In the food containing the anticancer composition of the present invention, the composition of the present invention described above may be contained in an amount of 0.001 to 100 weight percent, preferably 0.1 to 40 weight percent, based on the total weight of the food, and in the case of a beverage, 0.001 to 5g, preferably 0.01 to 2g, based on 100ml, but may be below the above range in the case of long-term administration for the purpose of health and hygiene or for the purpose of health regulation. The active ingredient is not at all problematic in terms of safety, and therefore, an amount of the active ingredient exceeding the above range may be used, and the amount is not limited to the above range.

Accordingly, the present invention can provide a health functional food for preventing or improving cancer diseases, which comprises the Rhus verniciflua Stokes extract of the present invention as an active ingredient, and the form of the food can be, but is not limited to, powder, granule, tablet, capsule or beverage.

On the other hand, the dosage form of the anticancer agent composition may be prepared in a preferred form according to the method of use, and in particular, in order to provide rapid, sustained or delayed release of the active ingredient after administration to a mammal, it is preferable to select a method known in the art to which the present invention pertains to perform the dosage form.

The formulation can be made into a dosage form selected from the group consisting of PLASTERS (PLASTERS), GRANULES (GRANULES), emollients (lotiones), Liniments (LINIMENTS), lemon water (LEMONADES), aromatic water (aromantcwaters), POWDERS (POWDERS), SYRUPS (SYRUPS), ophthalmic OINTMENTS (opthalmic OINTMENTS), LIQUIDS (LIQUIDS AND gels), sprays (AEROSOLS), extractants (extractants), tonics (ELIXIRS), OINTMENTS (ointements), FLUIDEXTRACTS (FLUIDEXTRACTS), EMULSIONS (emulisones), suspensions (suspersications), AND DECOCTIONS (DECOCTIONS), extract (INFUSIONS), eye ointment (OPHTHALMIC SOLUTIONS), tablet (TABLETS), suppository (SUPPOSITIORIES), injection (INJECTIONS), alcoholic preparation (spririts), Cataplasm (CATAPLSMA), Capsule (CAPSULES), Cream (CREAMS), troche (troche), tincture (TINCTURES), Paste (PASTES), pill (pils), soft or hard gelatin capsule, coated tablet, powder, granule, and capsule.

In the case of preparing a coated tablet, talc, hydroxypropylmethylcellulose, and polyethylene glycol may be added to a sumac extract containing fisetin as a main component and then mixed and tableted by a known method, or lactose, magnesium stearate, colloidal silicon dioxide, hydroxypropylmethylcellulose, polyethylene glycol, and titanium dioxide may be added and then mixed and tableted by a known method.

When the preparation is a powder, white sugar, lactose, and microcrystalline cellulose may be added to the sumac extract added fisetin as the main component, and the preparation may be carried out by a known method, or mannitol, corn starch, and colloidal silicon dioxide may be added to the main component, and the preparation may be carried out by a known method.

When the preparation is a granule, white sugar and corn starch may be added to the sumac extract added with fisetin as the main component, and the preparation may be carried out by a known method, or mannitol, lactose, povidone, or colloidal silicon dioxide may be added to the main component, and the preparation may be carried out by a known method.

In the case of preparing a capsule, microcrystalline cellulose, corn starch, hydroxypropyl cellulose, and magnesium stearate may be added to the sumac extract, which is the main component and contains fisetin, and then the capsule may be prepared by a known method, or lactose, povidone, colloidal silicon dioxide, talc, and magnesium stearate may be added to the main component and then the capsule may be prepared by a known method.

The dose of the anticancer composition of the present invention is preferably determined in consideration of the administration method, the age, sex, and weight of the person to be administered, the severity of the disease, and the like. For example, the anticancer composition of the present invention may be administered at a dose of 0.1mg/kg (body weight) to 100mg/kg (body weight) once a day on the basis of the active ingredient. However, the dose is only an example for illustration and is not limited to the above range, and the dose can be adjusted by a specialist in accordance with the severity of the patient and the condition of the patient.

The present invention will be described in more detail in the following examples. However, the scope of the invention is not limited to the following embodiments, and includes all modifications equivalent to the technical ideas.

Example 1

10kg of Korean sumac was purchased from the Kyoto market and sufficiently dried, 90L of water was added thereto, and extracted at a temperature of 95 ℃ for 6 hours, filtered, and vacuum-concentrated to obtain 580g of sumac extract powder (which is referred to as sample 1) after powdering.

100g of the above sample 1 was taken and dissolved in 5 times of water, heated to 95 ℃, and then 10g of chromium powder (100 mesh) as a catalyst was added thereto and reacted for 6 hours, and then 2000ml of alcohol was added and mixed in order to dissolve the fisetin formed as a precipitate, and after that insoluble matters including the catalyst were removed by filtration using a 0.5 μm filter, and concentrated and powdered, 93g (this was referred to as sample 2) was obtained.

Next, 100g of the above sample 1 was taken and dissolved in 5 times of water, heated to 95 ℃, and then 1g of a chromium catalyst supported on activated carbon (the content of supported chromium is 10% (w/w) by weight) as a catalyst was added thereto, and after 6 hours of reaction, 2000ml of alcohol was added and mixed in order to dissolve the fisetin formed as a precipitate, and then insoluble matter including the catalyst was removed by filtration using a 0.5 μm filter, and after concentration and powdering, 94.5g was obtained (this is referred to as sample 3).

Next, 6 parts of sample 1 (10 g) were taken, dissolved in 5 times of water, heated to 95 ℃ and then 1g of platinum or chromium oxide (Cr) was added as a catalyst₂O₃)、(NH₄)₂CrO₄Copper, nickel and silicon powders were reacted for 6 hours, and then 200ml of alcohol was added to dissolve the precipitated fisetin, followed by mixing, filtering with a 0.5 μm filter to remove insoluble matter including the catalyst, and concentrating and powdering the mixture to obtain 9.8g, 9.3g, 9.7g, 9.8g, 9.9g and 9.9g (these were referred to as sample 4, sample 5, sample 6, sample 7, sample 8 and sample 9, respectively).

Comparative example 1

To compare the difference from the prior art, 1kg of Rhus verniciflua Stokes was sufficiently dried, 10L of water was added thereto, extracted at 95 ℃ for 6 hours, filtered, and concentrated under vacuum in the same manner as in example 1, and when the concentration of the solid content reached 10.5Bx, 610ml of a concentrated solution was obtained, and treated at 90 ℃ for 3 hours while bubbling oxygen gas into a glass flask. Then, the powder was pulverized to obtain 57g (referred to as comparative example 1).

To analyze the content, high performance liquid chromatography was used to analyze the fustin and fisetin. The analysis conditions were those of generally known flavonoid compounds. The structure is shown in table 1.

TABLE 1

	Fustin (%)	Fisetin (%)	Conversion (%)
				Sample 1 (extract)	12.1	1.7	-
Sample 2 (chromium powder)	5.6	7.5	47.9
				Sample 3 (carrying chromium)	2.2	9.4	63.6
Sample 4 (platinum)	1.8	10.8	75.2
				Sample 5 (chromium oxide)	6.6	4.9	26.4
Sample 6 (ammonium chromate)	7.8	4.0	19.0
				Sample 7 (copper)	6.3	4.5	23.1
Sample 8 (Nickel)	7.0	3.7	16.5
				Sample 9 (silicon)	8.2	4.2	20.7
Comparative example 1 (oxygen treatment)	11.5	1.8	0.8

The conversion was calculated as (amount of increase in fisetin/initial content of fustin) × 100.

As shown in table 1, it was confirmed that the method described in the present invention exhibited an absolutely higher conversion rate when converting fustin into fisetin, as compared with the prior art.

Example 2

In order to compare the difference in conversion rate according to the step of adding the catalyst, in the same manner as in example 1, after preparing an extract from 10kg of sumac, 1L of the extract was filtered, and then concentrated to a solid content of 11.2% (w/v), a chromium catalyst supported on activated carbon (same as in example 3 of example 1) was added thereto in an amount of 1% (w/w) relative to the solid content, and after reacting in the same manner, 250ml of alcohol was added thereto and dissolved, and the mixture was powdered after filtering, and finally 57.3g of a sumac extract increased in fisetin was obtained. As a result of analysis using high performance liquid chromatography, the content of fisetin was 9.9% (w/w), and the conversion into fisetin was 68%.

Example 3

The anti-cancer activity increased according to fisetin was compared with the samples obtained as described above. Generally, methods for confirming the efficacy of inhibiting cancer metastasis mainly include methods of comparative attachment (attachment), Invasion (Invasion), and metastasis (Migration). In the present invention, the inhibitory effects on metastatic cancer were compared for samples 1 and 4. The results of comparison of the inhibition of metastasis by gastric cancer-derived cell AGS cells are shown in fig. 3. Sample 4, which is a sumac extract with an increased content of fisetin, showed a significantly improved result of inhibiting metastasis of AGS cells derived from gastric cancer cells, compared to sample 1, which is the non-treated group. It can be thus demonstrated that the sumac extract prepared according to the preparation method of the present invention exhibits an inhibitory effect on cancer metastasis.

Industrial applicability

The present invention relates to a method for preparing a sumac extract and a composition comprising the same, which increase the content of fisetin having a more potent anticancer activity, and can be developed into health functional foods, functional food materials, natural anticancer agents, etc., and can be developed into drugs having no side effects and having an effect of inhibiting cancer metastasis.