阅读说明：本技术 新型细菌lpp突变体及其在重组蛋白质的分泌生产中的用途 (Novel bacterial lpp mutants and their use in the secretory production of recombinant proteins ) 是由 T·达斯勒 M·库尧 于 2018-07-24 设计创作，主要内容包括：本发明涉及一种细菌菌株,其含有至少一种编码重组蛋白质的基因,其特征在于其含有由以下组成的开放阅读框：(i)编码介导所述蛋白质向周质的易位的N-末端信号肽的DNA片段,其连接至(ii)编码脂蛋白(Lpp(N))的后续DNA序列(lpp(N)),其与野生型lpp基因编码的脂蛋白Lpp相比,具有至多十个氨基酸的差异,以及(iii)编码脂蛋白(Lpp(C))的另一DNA序列(lpp(C)),其与野生型lpp基因编码的脂蛋白(Lpp)相比,具有至多十个氨基酸的差异。本发明还涉及一种用于使用根据本发明的细菌菌株发酵生产重组蛋白质的方法。如此,可能实现与现有技术相比培养基中的重组蛋白的增加的量。(The invention relates to a bacterial strain containing at least one gene coding for a recombinant protein, characterized in that it contains open reading frames consisting of: (i) a DNA fragment encoding an N-terminal signal peptide mediating translocation of the protein to the periplasm linked to (ii) a subsequent DNA sequence (Lpp (N)) encoding a lipoprotein (Lpp (N)) which differs by at most ten amino acids compared to the lipoprotein Lpp encoded by the wild-type Lpp gene, and (iii) another DNA sequence (Lpp (c)) encoding a lipoprotein (Lpp (c)) which differs by at most ten amino acids compared to the lipoprotein (Lpp) encoded by the wild-type Lpp gene. The invention also relates to a method for the fermentative production of recombinant proteins using the bacterial strain according to the invention. In this way, it is possible to achieve an increased amount of recombinant protein in the culture medium compared to the prior art.)