阅读说明：本技术 一种提高鱼虾抗病力的饲料添加剂、其制备方法及应用 (Feed additive for improving disease resistance of fishes and shrimps as well as preparation method and application thereof ) 是由 张亮 于 2021-01-20 设计创作，主要内容包括：本发明提供一种提高鱼虾抗病力的饲料添加剂,其中甘氨酸、黄芪多糖和水飞蓟素能够协同提高水产动物肝脏SOD的活性,促进水产动物胸腺、骨髓、淋巴结的增重和发育,保护肝细胞膜,有效提高水产动物的抗氧化能力和抗应激能力；破壁酵母粉具有诱食效果,能够提高鱼虾对其他饲料的利用率,促进鱼虾的快速生长；复合中草药乳液通过各组分相互作用,共同到达抗病毒、抗炎、调节免疫系统的功效。本发明通过甘氨酸、破壁酵母粉、黄芪多糖、水飞蓟素、复合中草药乳液和黄原胶共同作用,协同达到抗炎、杀菌、提高鱼虾免疫力的功效,有效提高鱼虾的抗病力。本发明提供的饲料添加剂,不含激素,不会产生抗药性,具有保肝健肠、消炎抗菌、利胆促长的功效。(The invention provides a feed additive for improving disease resistance of fishes and shrimps, wherein glycine, astragalus polysaccharide and silymarin can synergistically improve the activity of SOD (superoxide dismutase) of livers of aquatic animals, promote the weight increment and development of thymus, bone marrow and lymph nodes of the aquatic animals, protect liver cell membranes and effectively improve the oxidation resistance and stress resistance of the aquatic animals; the wall-broken yeast powder has a food calling effect, can improve the utilization rate of other feeds for fishes and shrimps and promote the quick growth of the fishes and shrimps; the composite Chinese herbal medicine emulsion achieves the effects of resisting virus, resisting inflammation and regulating an immune system together through the interaction of all components. The invention synergistically achieves the effects of resisting inflammation, sterilizing and improving the immunity of fishes and shrimps by the combined action of glycine, wall-broken yeast powder, astragalus polysaccharide, silymarin, composite Chinese herbal medicine emulsion and xanthan gum, and effectively improves the disease resistance of fishes and shrimps. The feed additive provided by the invention does not contain hormone, does not generate drug resistance, and has the effects of protecting liver and intestines, diminishing inflammation and resisting bacteria, and benefiting gallbladder and promoting growth.)

1. The feed additive for improving the disease resistance of fishes and shrimps is characterized by comprising the following components in parts by weight:

1-10 parts of glycine, 1-10 parts of wall-broken yeast powder, 1-10 parts of astragalus polysaccharide, 1-3 parts of silymarin, 20-85 parts of composite Chinese herbal medicine emulsion and 0.1-3 parts of xanthan gum;

the composite Chinese herbal medicine emulsion comprises the following raw materials in parts by weight:

1-8 parts of a radix bupleuri extract, 1-5 parts of a bighead atractylodes rhizome extract, 1-8 parts of a schisandra chinensis extract, 1-5 parts of a licorice extract, 1-5 parts of a medlar extract, 1-5 parts of a oriental wormwood extract, 1-8 parts of a honeysuckle extract, 1-5 parts of a folium isatidis extract, 1-5 parts of a radix sileris extract, 1-5 parts of a fennel extract, 0-30 parts of tween 8010, 1-10 parts of alkyl glycoside and 10-20 parts of pure water.

2. The feed additive for improving disease resistance of fishes and shrimps as claimed in claim 1, wherein the preparation method of the compound traditional Chinese medicine emulsion comprises the following steps:

mixing Tween 80 and alkyl glucoside, adding water, uniformly stirring, heating to 40-50 ℃, adding the rest raw materials, and keeping the temperature at 40-50 ℃ for 3-5 hours to obtain the composite Chinese herbal medicine emulsion.

3. The feed additive for improving disease resistance of fishes and shrimps as claimed in claim 1, wherein the preparation method of the bupleurum extract in the composite traditional Chinese medicine emulsion comprises the following steps:

adding radix bupleuri into 6-8 times of water, soaking for 4-6 hours, extracting volatile oil under reflux to obtain filter residue, adding the filter residue into 3-5 times of water, decocting for 2-3 times for 1-2 hours each time, filtering, combining filtrates, concentrating to obtain a concentrated solution, adding 1-2 times of ethanol solution with the weight concentration of 55-65% into the concentrated solution, soaking for 30-60min, performing suction filtration to obtain a precipitate, adding 1-2 times of water, filtering, and drying to obtain the radix bupleuri extract.

4. The feed additive for improving disease resistance of fishes and shrimps as claimed in claim 1, wherein the preparation method of the bighead atractylodes rhizome extract in the composite traditional Chinese medicine emulsion comprises the following steps:

taking and crushing the bighead atractylodes rhizome to obtain bighead atractylodes rhizome powder with the particle size of 20-100 meshes, adding the powder into ethyl acetate with the weight of 4-5 times of the powder, performing reflux extraction for 60-80 min, performing suction filtration to obtain filtrate, and concentrating to obtain bighead atractylodes rhizome volatile oil; adding filter residues into warm water at the temperature of 40-45 ℃, performing reflux extraction, filtering, concentrating to obtain a concentrated solution, adding ethanol into the concentrated solution, performing suction filtration and drying to obtain atractylodes macrocephala polysaccharide, and mixing the atractylodes macrocephala volatile oil and the atractylodes macrocephala polysaccharide to obtain the atractylodes macrocephala extract.

5. The feed additive for improving disease resistance of fish and shrimp, which is provided by claim 1, is characterized in that the preparation method of the schisandra extract in the compound traditional Chinese medicine emulsion comprises the following steps: crushing fructus schisandrae, adding into an extraction kettle, and performing CO extraction at the pressure of 25-35 MPa and the temperature of 35-40 DEG C₂Extracting under the condition that the flow is 15-20L/h, and drying to obtain the schisandra extract.

6. The feed additive for improving disease resistance of fishes and shrimps as claimed in claim 1, wherein the preparation method of the artemisia capillaris extract in the compound traditional Chinese medicine emulsion comprises the following steps: taking herba artemisiae scopariae, adding the herba artemisiae scopariae into methanol which is 3-5 times of the amount of the herba artemisiae scopariae, soaking for 5-10 hours, distilling and extracting for 2-3 times, combining filtrates, concentrating, adding an ethanol solution, carrying out spin drying to obtain an extract, and drying to obtain the herba artemisiae scopariae extract.

7. The feed additive for improving disease resistance of fishes and shrimps as claimed in claim 1, wherein the preparation method of the honeysuckle extract in the composite traditional Chinese medicine emulsion comprises the following steps: adding 8-10 times of water into honeysuckle, distilling and extracting for 2-3 times, combining filtrates, concentrating under reduced pressure until the relative density is 1.12-1.15, adding 2-3 times of ethanol, refrigerating at 0-5 ℃ for 12-24 h, filtering, concentrating under reduced pressure, and drying to obtain the honeysuckle extract.

8. The feed additive for improving disease resistance of fishes and shrimps as claimed in claim 1, wherein the preparation method of the saposhnikovia divaricata extract in the composite traditional Chinese medicine emulsion comprises the following steps:

taking and crushing divaricate saposhnikovia root, adding the crushed divaricate saposhnikovia root into 5-8 times of methanol, performing reflux extraction for 2-3 h, concentrating, and drying to obtain the divaricate saposhnikovia root extract.

9. The method for preparing a feed additive for improving disease resistance of fish and shrimp according to any one of claims 1-8, comprising the steps of:

and (3) adding other components into the Chinese herbal medicine emulsion, uniformly mixing, and granulating to obtain the feed additive.

10. The use of the feed additive according to any one of claims 1 to 8 for improving disease resistance of fish and shrimp in the preparation of antiviral feed for fish and shrimp.

Technical Field

The invention belongs to the technical field of aquaculture, and particularly relates to a feed additive for improving disease resistance of fishes and shrimps, and a preparation method and application thereof.

Background

In the process of aquaculture, fishes and shrimps are often invaded by bacteria, viruses and the like, so that the fishes and shrimps die in a large amount or the quality of the fishes and shrimps is reduced. In the traditional fish and shrimp culture process, a large amount of antibiotics are used for preventing and treating fish and shrimp diseases, and the survival rate of the fish and shrimp is improved. However, antibiotics are easy to remain in bodies of fishes and shrimps, so that the quality of the fishes and shrimps is reduced, and the antibiotics are easy to cause drug resistance of the fishes and shrimps after being used for a long time, and pollute the water area, so that ecological imbalance is caused, and vicious circle is caused.

Disclosure of Invention

In order to solve the technical problems, the invention provides a feed additive for improving disease resistance of fishes and shrimps, which does not contain hormone, does not generate drug resistance, and has the effects of protecting liver and intestines, diminishing inflammation, resisting bacteria, benefiting gallbladder and promoting growth.

The invention provides a feed additive for improving disease resistance of fishes and shrimps, which comprises the following components in parts by weight:

1-10 parts of glycine, 1-10 parts of wall-broken yeast powder, 1-10 parts of astragalus polysaccharide, 1-3 parts of silymarin, 20-85 parts of composite Chinese herbal medicine emulsion and 0.1-3 parts of xanthan gum;

the composite Chinese herbal medicine emulsion comprises the following raw materials in parts by weight:

1-8 parts of a radix bupleuri extract, 1-5 parts of a bighead atractylodes rhizome extract, 1-8 parts of a schisandra chinensis extract, 1-5 parts of a licorice extract, 1-5 parts of a medlar extract, 1-5 parts of a oriental wormwood extract, 1-8 parts of a honeysuckle extract, 1-5 parts of a folium isatidis extract, 1-5 parts of a radix sileris extract, 1-5 parts of a fennel extract, 0-30 parts of tween 8010, 1-10 parts of alkyl glycoside (APG 0810) and 10-20 parts of pure water.

The glycine, the astragalus polysaccharide and the silymarin in the feed additive for improving the disease resistance of the fishes and shrimps provided by the invention can synergistically improve the activity of the liver SOD of the aquatic animals, promote the weight increment and development of thymus, bone marrow and lymph nodes of the aquatic animals, protect liver cell membranes and effectively improve the oxidation resistance and stress resistance of the aquatic animals; the wall-broken yeast powder has a food calling effect, can improve the utilization rate of other feeds for fishes and shrimps and promote the quick growth of the fishes and shrimps; the composite Chinese herbal medicine emulsion achieves the effects of resisting virus, resisting inflammation and regulating an immune system together through the interaction of all components. The invention synergistically achieves the effects of resisting inflammation, sterilizing and improving the immunity of fishes and shrimps by the combined action of glycine, wall-broken yeast powder, astragalus polysaccharide, silymarin, composite Chinese herbal medicine emulsion and xanthan gum, thereby effectively improving the disease resistance of fishes and shrimps.

Preferably, the preparation method of the compound traditional Chinese medicine emulsion comprises the following steps:

mixing Tween 80 and alkyl glucoside, adding water, uniformly stirring, heating to 40-50 ℃, adding the rest raw materials, and keeping the temperature at 40-50 ℃ for 3-5 hours to obtain the composite Chinese herbal medicine emulsion.

The composite traditional Chinese medicine emulsion provided by the invention is uniform and stable through the method.

Preferably, the preparation method of the bupleurum extract in the composite traditional Chinese medicine emulsion comprises the following steps:

adding radix bupleuri into 6-8 times of water, soaking for 4-6 hours, extracting volatile oil under reflux to obtain filter residues, adding the filter residues into 3-5 times of water, decocting for 2-3 times for 1-2 hours each time, filtering, combining filtrates, concentrating to obtain a concentrated solution, adding 1-2 times of ethanol solution with the weight of 55-65% into the concentrated solution, soaking for 30-60 minutes, performing suction filtration to obtain a precipitate, adding 1-2 times of water, filtering, and drying to obtain the radix bupleuri extract.

The preparation method of the bupleurum extract provided by the invention is carried out by firstly extracting volatile oil from bupleurum and then extracting with 55-65% ethanol to obtain saikosaponin, wherein the yield of the saikosaponin is high and is close to 5%. The saikosaponin has antiinflammatory, antiviral, and immune system regulating effects.

Preferably, the preparation method of the atractylodes macrocephala koidz extract in the compound traditional Chinese medicine emulsion comprises the following steps:

taking and crushing the bighead atractylodes rhizome to obtain bighead atractylodes rhizome powder with the particle size of 20-100 meshes, adding the powder into ethyl acetate with the weight of 4-5 times of the powder, performing reflux extraction for 60-80 min, performing suction filtration to obtain filtrate, and concentrating to obtain bighead atractylodes rhizome volatile oil; adding filter residues into warm water at the temperature of 40-45 ℃, performing reflux extraction, filtering, concentrating to obtain a concentrated solution, adding ethanol into the concentrated solution, performing suction filtration and drying to obtain atractylodes macrocephala polysaccharide, and mixing the atractylodes macrocephala volatile oil and the atractylodes macrocephala polysaccharide to obtain the atractylodes macrocephala extract.

The preparation method of the bighead atractylodes rhizome extract provided by the invention uses ethyl acetate as a solvent for reflux extraction to obtain bighead atractylodes rhizome volatile oil, and adopts a method of warm water extraction and ethanol precipitation to obtain bighead atractylodes rhizome polysaccharide. The bighead atractylodes rhizome extract provided by the invention contains bighead atractylodes rhizome volatile oil and bighead atractylodes rhizome polysaccharide, the bighead atractylodes rhizome volatile oil has the effects of enhancing non-specific immunity of organisms, adjusting gastrointestinal tract and promoting nutrient absorption, and the bighead atractylodes rhizome polysaccharide has the effects of resisting viruses and enhancing immunity.

Preferably, the preparation method of the schisandra extract in the composite traditional Chinese medicine emulsion comprises the following steps: crushing fructus schisandrae, adding into an extraction kettle, and performing CO extraction at the pressure of 25-35 MPa and the temperature of 35-40 DEG C₂Extracting under the condition that the flow is 15-20L/h, and drying to obtain the schisandra extract.

The schisandra extract provided by the invention contains schisandra volatile oil, organic acid, schizandrin A and schizandrin B, and has the effects of protecting liver and resisting bacteria.

Preferably, the preparation method of the artemisia capillaris extract in the composite traditional Chinese medicine emulsion comprises the following steps: taking herba artemisiae scopariae, adding the herba artemisiae scopariae into methanol which is 3-5 times of the amount of the herba artemisiae scopariae, soaking for 5-10 hours, distilling and extracting for 2-3 times, combining filtrates, concentrating, adding an ethanol solution, carrying out spin drying to obtain an extract, and drying to obtain the herba artemisiae scopariae extract.

The herba artemisiae scopariae extract provided by the invention contains sterol, azelaic acid, hexadecanoic acid and quercetin, and has a good antibacterial effect; contains no chlorophyll, and avoids dyeing water body.

Preferably, the preparation method of the honeysuckle extract in the composite traditional Chinese medicine emulsion comprises the following steps: adding 8-10 times of water into honeysuckle, distilling and extracting for 2-3 times, combining filtrates, concentrating under reduced pressure until the relative density is 1.12-1.15, adding 2-3 times of ethanol, refrigerating at 0-5 ℃ for 12-24 h, filtering, concentrating under reduced pressure, and drying to obtain the honeysuckle extract.

The honeysuckle extract provided by the invention is rich in chlorogenic acid, luteolin and saponin, has broad-spectrum antibacterial property and antiviral property, can regulate the immunity of an organism, improve the immunity of the organism, enhance the phagocytic function of leucocytes, promote the release of adrenocortical hormone and has an inhibiting effect on acute inflammation.

Preferably, the preparation method of the divaricate saposhnikovia root extract in the composite traditional Chinese medicine emulsion comprises the following steps:

taking and crushing divaricate saposhnikovia root, adding the crushed divaricate saposhnikovia root into 5-8 times of methanol, performing reflux extraction for 2-3 h, concentrating, and drying to obtain the divaricate saposhnikovia root extract.

The radix sileris extract provided by the invention contains the substances such as anti-rubella ketol, cimicifuga glycoside and the like, and has the effects of relieving fever, easing pain and resisting inflammation.

The invention provides a preparation method of the feed additive for improving disease resistance of fishes and shrimps, which comprises the following steps:

and (3) adding other components into the Chinese herbal medicine emulsion, uniformly mixing, and granulating to obtain the feed additive.

The feed additive for improving the disease resistance of the fishes and the shrimps, provided by the invention, can be used for not only a feed and improving the disease resistance of the fishes and the shrimps, but also can be used for preparing an antiviral feed for the fishes and the shrimps.

The feed additive for improving the disease resistance of the fishes and shrimps, provided by the invention, is used in an amount of 2-4 wt% in the feed.

The invention has the beneficial effects that:

1. the glycine, the astragalus polysaccharide and the silymarin in the feed additive for improving the disease resistance of the fishes and shrimps provided by the invention can synergistically improve the activity of the liver SOD of the aquatic animals, promote the weight increment and development of thymus, bone marrow and lymph nodes of the aquatic animals, protect liver cell membranes and effectively improve the oxidation resistance and stress resistance of the aquatic animals; the wall-broken yeast powder has a food calling effect, can improve the utilization rate of other feeds for fishes and shrimps and promote the quick growth of the fishes and shrimps; the composite Chinese herbal medicine emulsion achieves the effects of resisting virus, resisting inflammation and regulating an immune system together through the interaction of all components. The invention synergistically achieves the effects of resisting inflammation, sterilizing and improving the immunity of fishes and shrimps by the combined action of glycine, wall-broken yeast powder, astragalus polysaccharide, silymarin, composite Chinese herbal medicine emulsion and xanthan gum, thereby effectively improving the disease resistance of fishes and shrimps.

2. The feed additive provided by the invention does not contain hormone, does not generate drug resistance, and has the effects of protecting liver and intestines, diminishing inflammation and resisting bacteria, and benefiting gallbladder and promoting growth.

Detailed Description

In order to make the objects, technical solutions and advantages of the present invention more apparent, the technical solutions of the present invention will be described in detail below. It is to be understood that the described embodiments are merely exemplary of the invention, and not restrictive of the full scope of the invention. All other embodiments, which can be derived by a person skilled in the art from the examples given herein without any inventive step, are within the scope of the present invention.

In the examples of the present invention, 10g was used per 1 part by weight.

Example 1

A feed additive for improving disease resistance of fishes and shrimps comprises the following components in parts by weight:

1 part of glycine, 1 part of broken yeast powder, 1 part of astragalus polysaccharide, 1 part of silymarin, 20 parts of composite Chinese herbal medicine emulsion and 0.1 part of xanthan gum;

the composite Chinese herbal medicine emulsion comprises the following raw materials in parts by weight:

1 part of bupleurum extract, 1 part of atractylodes extract, 1 part of schisandra extract, 1 part of licorice extract, 1 part of medlar extract, 1 part of oriental wormwood extract, 1 part of honeysuckle extract, 1 part of dyers woad leaf extract, 1 part of divaricate saposhnikovia root extract, 1 part of fennel extract, tween 8010 part, 1 part of alkyl glycoside (APG 0810) and 10 parts of pure water.

The preparation method of the composite traditional Chinese medicine emulsion comprises the following steps:

mixing tween 80 and alkyl glycoside, adding water, stirring, heating to 40 deg.C, adding the rest raw materials, and keeping the temperature at 40 deg.C for 5 hr to obtain the compound Chinese medicinal emulsion.

The preparation method of the bupleurum extract in the composite traditional Chinese medicine emulsion comprises the following steps:

adding radix bupleuri into 6 times of water, soaking for 4h, extracting volatile oil under reflux to obtain residue, adding the residue into 3 times of water, decocting for 2 times (1 h each time), filtering, mixing filtrates, concentrating to obtain concentrated solution, adding 1 time of 55% ethanol solution, soaking for 30min, filtering to obtain precipitate, adding 1 time of water, filtering, and drying to obtain radix bupleuri extract.

The preparation method of the bighead atractylodes rhizome extract in the composite traditional Chinese medicine emulsion comprises the following steps:

pulverizing Atractylodis rhizoma to obtain Atractylodis rhizoma powder with particle size of 20-100 meshes, adding into 4 times of ethyl acetate, reflux extracting for 60min, vacuum filtering to obtain filtrate, and concentrating to obtain Atractylodis rhizoma volatile oil; adding the filter residue into warm water at 40 deg.C, reflux extracting, filtering, concentrating to obtain concentrated solution, adding ethanol into the concentrated solution, vacuum filtering, drying to obtain Atractylodis rhizoma polysaccharide, mixing the Atractylodis rhizoma volatile oil and Atractylodis rhizoma polysaccharide to obtain Atractylodis rhizoma extract.

The preparation method of the schisandra extract in the composite traditional Chinese medicine emulsion comprises the following steps: pulverizing fructus Schisandrae, adding into extraction kettle under pressure of 25MPa and temperature of 35 deg.C and CO₂Extracting at flow rate of 15L/h, and drying to obtain fructus Schisandrae extract.

The preparation method of the herba artemisiae scopariae extract in the composite traditional Chinese medicine emulsion comprises the following steps: adding herba Artemisiae Scopariae into 3 times of methanol, soaking for 5 hr, distilling and extracting for 2 times, mixing filtrates, concentrating, adding ethanol solution, spin drying to obtain extract, and drying to obtain the herba Artemisiae Scopariae extract.

The preparation method of the honeysuckle extract in the composite traditional Chinese medicine emulsion comprises the following steps: adding 8 times of water into flos Lonicerae, distilling for 2 times, mixing filtrates, concentrating under reduced pressure to relative density of 1.12, adding 2-3 times of ethanol, refrigerating at 0 deg.C for 12 hr, filtering, concentrating under reduced pressure, and drying to obtain flos Lonicerae extract.

The preparation method of the divaricate saposhnikovia root extract in the composite traditional Chinese medicine emulsion comprises the following steps:

pulverizing radix Saposhnikoviae, adding into 5 times of methanol, reflux extracting for 2 hr, concentrating, and drying to obtain radix Saposhnikoviae extract.

The preparation method of the feed additive for improving the disease resistance of the fishes and the shrimps comprises the following steps:

and (3) adding other components into the Chinese herbal medicine emulsion, uniformly mixing, and granulating to obtain the feed additive.

Example 2

A feed additive for improving disease resistance of fishes and shrimps comprises the following components in parts by weight:

10 parts of glycine, 10 parts of broken yeast powder, 10 parts of astragalus polysaccharide, 3 parts of silymarin, 85 parts of composite Chinese herbal medicine emulsion and 0.3 part of xanthan gum;

the composite Chinese herbal medicine emulsion comprises the following raw materials in parts by weight:

8 parts of bupleurum extract, 5 parts of atractylodes macrocephala koidz extract, 8 parts of schisandra chinensis extract, 5 parts of liquorice extract, 5 parts of medlar extract, 5 parts of oriental wormwood extract, 8 parts of honeysuckle extract, 5 parts of folium isatidis extract, 5 parts of divaricate saposhnikovia root extract, 5 parts of fennel extract, tween 8030, 10 parts of alkyl glycoside (APG 0810) and 20 parts of pure water.

The preparation method of the composite traditional Chinese medicine emulsion comprises the following steps:

mixing tween 80 and alkyl glycoside, adding water, stirring, heating to 50 deg.C, adding the rest raw materials, and keeping the temperature at 50 deg.C for 5 hr to obtain the compound Chinese medicinal emulsion.

The preparation method of the bupleurum extract in the composite traditional Chinese medicine emulsion comprises the following steps:

adding radix bupleuri into 8 times of water, soaking for 6h, extracting volatile oil under reflux to obtain residue, adding the residue into 5 times of water, decocting for 3 times, each time for 2h, filtering, mixing filtrates, concentrating to obtain concentrated solution, adding 2 times of 65% ethanol solution into the concentrated solution, soaking for 60min, filtering to obtain precipitate, adding 2 times of water, filtering, and drying to obtain radix bupleuri extract.

The preparation method of the bighead atractylodes rhizome extract in the composite traditional Chinese medicine emulsion comprises the following steps:

pulverizing Atractylodis rhizoma to obtain Atractylodis rhizoma powder with particle size of 20-100 meshes, adding into 5 times of ethyl acetate, reflux extracting for 80min, vacuum filtering to obtain filtrate, and concentrating to obtain Atractylodis rhizoma volatile oil; adding the filter residue into warm water at 45 deg.C, reflux extracting, filtering, concentrating to obtain concentrated solution, adding ethanol into the concentrated solution, vacuum filtering, drying to obtain Atractylodis rhizoma polysaccharide, mixing the Atractylodis rhizoma volatile oil and Atractylodis rhizoma polysaccharide to obtain Atractylodis rhizoma extract.

The preparation method of the schisandra extract in the composite traditional Chinese medicine emulsion comprises the following steps: pulverizing fructus Schisandrae, adding into extraction kettle under 35MPa and 40 deg.C with CO₂Extracting at flow rate of 20L/h, and drying to obtain fructus Schisandrae extract.

The preparation method of the herba artemisiae scopariae extract in the composite traditional Chinese medicine emulsion comprises the following steps: adding herba Artemisiae Scopariae into 5 times of methanol, soaking for 10 hr, distilling and extracting for 3 times, mixing filtrates, concentrating, adding ethanol solution, spin drying to obtain extract, and drying to obtain the herba Artemisiae Scopariae extract.

The preparation method of the honeysuckle extract in the composite traditional Chinese medicine emulsion comprises the following steps: adding 10 times of water into flos Lonicerae, distilling and extracting for 3 times, mixing filtrates, concentrating under reduced pressure to relative density of 1.15, adding 3 times of ethanol, refrigerating at 5 deg.C for 24 hr, filtering, concentrating under reduced pressure, and drying to obtain flos Lonicerae extract.

The preparation method of the divaricate saposhnikovia root extract in the composite traditional Chinese medicine emulsion comprises the following steps:

pulverizing radix Saposhnikoviae, adding into 8 times of methanol, reflux extracting for 3 hr, concentrating, and drying to obtain radix Saposhnikoviae extract.

The preparation method of the feed additive for improving the disease resistance of the fishes and the shrimps comprises the following steps:

and (3) adding other components into the Chinese herbal medicine emulsion, uniformly mixing, and granulating to obtain the feed additive.

Example 3

A feed additive for improving disease resistance of fishes and shrimps comprises the following components in parts by weight:

8 parts of glycine, 8 parts of broken yeast powder, 8 parts of astragalus polysaccharide, 2 parts of silymarin, 60 parts of composite Chinese herbal medicine emulsion and 0.5 part of xanthan gum;

the composite Chinese herbal medicine emulsion comprises the following raw materials in parts by weight:

5 parts of bupleurum extract, 3 parts of atractylodes extract, 3 parts of schisandra extract, 3 parts of licorice extract, 3 parts of medlar extract, 3 parts of oriental wormwood extract, 3 parts of honeysuckle extract, 3 parts of dyers woad leaf extract, 3 parts of divaricate saposhnikovia root extract, 3 parts of fennel extract, tween 8025, 8 parts of alkyl glycoside (APG 0810) and 15 parts of pure water.

The preparation method of the composite traditional Chinese medicine emulsion comprises the following steps:

mixing tween 80 and alkyl glycoside, adding water, stirring, heating to 45 deg.C, adding the rest raw materials, and keeping the temperature at 45 deg.C for 4 hr to obtain the compound Chinese herbal medicine emulsion.

The preparation method of the bupleurum extract in the composite traditional Chinese medicine emulsion comprises the following steps:

adding radix bupleuri into 6 times of water, soaking for 5h, extracting volatile oil under reflux to obtain residue, adding the residue into 4 times of water, decocting for 2 times (1.5 h each time), filtering, mixing filtrates, concentrating to obtain concentrated solution, adding 1.5 times of 60% ethanol solution into the concentrated solution, soaking for 45min, vacuum filtering to obtain precipitate, adding 1.5 times of water, filtering, and drying to obtain radix bupleuri extract.

The preparation method of the bighead atractylodes rhizome extract in the composite traditional Chinese medicine emulsion comprises the following steps:

pulverizing Atractylodis rhizoma to obtain Atractylodis rhizoma powder with particle size of 20-100 meshes, adding into 4 times of ethyl acetate, reflux extracting for 60min, vacuum filtering to obtain filtrate, and concentrating to obtain Atractylodis rhizoma volatile oil; adding the filter residue into warm water at 40 deg.C, reflux extracting, filtering, concentrating to obtain concentrated solution, adding ethanol into the concentrated solution, vacuum filtering, drying to obtain Atractylodis rhizoma polysaccharide, mixing the Atractylodis rhizoma volatile oil and Atractylodis rhizoma polysaccharide to obtain Atractylodis rhizoma extract.

In the compoundingThe preparation method of the schisandra extract in the medicine emulsion comprises the following steps: pulverizing fructus Schisandrae, adding into extraction kettle under 30MPa and 35 deg.C with CO₂Extracting at flow rate of 15L/h, and drying to obtain fructus Schisandrae extract.

The preparation method of the herba artemisiae scopariae extract in the composite traditional Chinese medicine emulsion comprises the following steps: adding herba Artemisiae Scopariae into 4 times of methanol, soaking for 8 hr, distilling and extracting for 2 times, mixing filtrates, concentrating, adding ethanol solution, spin drying to obtain extract, and drying to obtain the herba Artemisiae Scopariae extract.

The preparation method of the honeysuckle extract in the composite traditional Chinese medicine emulsion comprises the following steps: adding 9 times of water into flos Lonicerae, distilling for 2 times, mixing filtrates, concentrating under reduced pressure to relative density of 1.14, adding 2 times of ethanol, refrigerating at 2 deg.C for 20 hr, filtering, concentrating under reduced pressure, and drying to obtain flos Lonicerae extract.

The preparation method of the divaricate saposhnikovia root extract in the composite traditional Chinese medicine emulsion comprises the following steps:

pulverizing radix Saposhnikoviae, adding into 6 times of methanol, reflux extracting for 2.5 hr, concentrating, and drying to obtain radix Saposhnikoviae extract.

The preparation method of the feed additive for improving the disease resistance of the fishes and the shrimps comprises the following steps:

and (3) adding other components into the Chinese herbal medicine emulsion, uniformly mixing, and granulating to obtain the feed additive.

Comparative example 1

A fish and shrimp feed additive comprises the following components in parts by weight:

8 parts of glycine, 8 parts of broken yeast powder, 8 parts of astragalus polysaccharide, 2 parts of silymarin, 0.5 part of xanthan gum and 10 parts of water;

the preparation method of the feed additive for improving the disease resistance of the fishes and the shrimps comprises the following steps:

and (3) taking water, adding other components, uniformly mixing, and granulating to obtain the feed additive.

Comparative example 2

A fish and shrimp feed additive comprises the following components in parts by weight:

8 parts of glycine, 8 parts of astragalus polysaccharide, 60 parts of composite Chinese herbal medicine emulsion and 0.5 part of xanthan gum;

the composite Chinese herbal medicine emulsion comprises the following raw materials in parts by weight:

5 parts of bupleurum extract, 3 parts of atractylodes macrocephala koidz extract, 3 parts of schisandra chinensis extract, 3 parts of liquorice extract, 3 parts of medlar extract, 3 parts of honeysuckle extract, 3 parts of folium isatidis extract, 8025 parts of tween, 8 parts of alkyl glycoside (APG 0810) and 10 parts of pure water.

The preparation method of the fish and shrimp feed additive comprises the following steps:

and (3) adding other components into the Chinese herbal medicine emulsion, uniformly mixing, and granulating to obtain the feed additive.

Test examples

1. Preparing 6 parts of the same solid culture medium, respectively adding 2 wt% of the feed additives obtained in the examples 1-3 and the comparative examples 1-2 before the solid culture medium is solidified, not adding the final group, respectively pouring each part of the culture medium into 10 culture dishes, and sterilizing; these 6 groups were designated test group 1, test group 2, test group 3, control group 1, control group 2 and blank control group, respectively. The inoculation concentration on each medium was 2.5X 10⁴cfu/mL of staphylococcus aureus, then after culturing for 18h at 37 ℃, counting the number of colonies on each culture medium, calculating the concentration of the staphylococcus aureus on each culture medium, averaging, and calculating the antibacterial rate, wherein the results are shown in Table 1.

Wherein the antibacterial rate is (viable bacteria concentration after 18h of culture after the blank control group is contacted with the bacteria, viable bacteria concentration after 18h of culture after each group is contacted with the bacteria colony)/viable bacteria concentration after 18h of culture after the blank control group is contacted with the bacteria is multiplied by 100 percent

TABLE 1 results of antimicrobial Properties of each group

As can be seen from the results in table 1, the antibacterial rate of the feed additive provided by the present invention against staphylococcus aureus reaches more than 97%, the antibacterial rate of the comparative example 1 obtained after removing the composite traditional Chinese medicine emulsion is greatly reduced to 83.33%, the antibacterial rate of the feed additive of the comparative example 2 obtained after removing the wall-broken yeast powder, silymarin, and the oriental wormwood extract, the divaricate saposhnikovia root extract and the fennel extract in the composite traditional Chinese medicine emulsion is only 67.5%, and the antibacterial rates of the comparative example 1 and the comparative example 2 are greatly reduced compared with the antibacterial rates of the feed additives obtained in the embodiments 1 to 3 of the present invention, which indicates that the feed additive provided by the present invention can synergistically achieve the antibacterial efficacy through the interaction among the components.

2. Selecting 5 fishponds with the same size and water quality, wherein the area of each fishpond is 66m²The left and the right are named as No. 1 fishpond, No. 2 fishpond, No. 3 fishpond, No. 4 fishpond and No. 5 fishpond respectively. 1.2 thousands of fish tails of carp fries with the same quantity are put in each fishpond and cultured for 10 days, wherein the feed additives obtained in the examples 1-3 are respectively put in the No. 1-3 fishpond along with the feed, the feed additive of the comparative example 1 is put in the No. 4 fishpond along with the feed, the feed additive of the comparative example 2 is put in the No. 5 fishpond along with the feed, the feed and the feed additive put in each fishpond are the same, the survival rate and the average weight gain rate of the fish in each fishpond are counted, and the results are shown in the table 2.

TABLE 2 survival rate of fish in each pond

Group of	Survival rate (%)
		No. 1 fishpond	98.56
No. 2 fishpond	97.36
		No. 3 fishpond	99.12
No. 4 fishpond	87.21
		No. 5 fishpond	75.65

As can be seen from the results in table 1, the survival rate of the carp fry in the fish pond nos. 1 to 3 is more than 97%, while the survival rate of the carp fry in the fish pond nos. 4 and 5 is significantly lower than that in the fish pond nos. 1, 2 and 3; the feed additive provided by the invention can effectively improve the disease resistance of fish and shrimp and improve the survival rate of fish and shrimp.

Typical case

In the carp pond, the carp of 2 years old has the symptoms of obviously reduced food intake, fish body emaciation and slow growth, and the death phenomenon begins to appear. The fish body has swelling intestinal wall, a large amount of parasites and inflamed intestinal wall through dissection, and the fish is diagnosed with the echinococcosis carpi. The feed additive obtained in the embodiment 3 of the invention is put into a fish pond along with feed, the dosage of the feed additive is 3.5 wt% of the feed, the feed is fed for 3 times every day, the feed quantity of carps is obviously improved after the feed is continuously fed for 10 days, and no death phenomenon occurs after the feed is fed for 3 days. The feed additive for improving the disease resistance of the fishes and the shrimps, which is provided by the invention, has the effects of protecting liver and strengthening intestines, diminishing inflammation and resisting bacteria, antagonizing viruses, benefiting gallbladder and promoting growth, and obviously improves the disease resistance of the fishes and the shrimps.

The above description is only for the specific embodiments of the present invention, but the scope of the present invention is not limited thereto, and any person skilled in the art can easily conceive of the changes or substitutions within the technical scope of the present invention, and all the changes or substitutions should be covered within the scope of the present invention. Therefore, the protection scope of the present invention shall be subject to the protection scope of the appended claims.