阅读说明：本技术 一种天然植物生物酶转化防治痤疮外用制剂及其制备方法和应用 (External preparation for preventing and treating acne through conversion of natural plant biological enzyme and preparation method and application thereof ) 是由 蒋用 何正有 汤秀强 李佳 毕建军 高鑫 于 2020-12-28 设计创作，主要内容包括：本发明公开了一种天然植物生物酶转化防治痤疮外用制剂及其制备方法和应用,属于化妆品技术领域；所述外用制剂按重量份计,包括：苦参10-20份、丹参10-20份、莼菜10-20份、积雪草10-20份、银杏5-15份、艾叶5-15份、马齿苋5-10份、山竹5-10份、黄芩5-10份、牡丹皮5-10份、甘草2-5份、麝香草2-5份和薄荷醇1-2份,本发明的配方科学,通过生物酶技术转换,提高植物生物利用度,获得疗效较高具有祛痘、杀菌消炎、控油调理肌肤、修复疤痕的植物配方,并且调配出依据皮肤生理特点适应不同肤质患者的使用制剂,在消除痤疮后皮肤无疤痕,无毒副作用,使用方便,能有效改善皮肤微循环,平衡油脂分泌,增强细胞新陈代谢,彻底清除皮肤内的垃圾和毒素,使皮肤炎症迅速消除。(The invention discloses an external preparation for preventing and treating acne through natural plant biological enzyme conversion, a preparation method and application thereof, belonging to the technical field of cosmetics; the external preparation comprises the following components in parts by weight: 10-20 parts of radix sophorae flavescentis, 10-20 parts of salvia miltiorrhiza, 10-20 parts of water shield, 10-20 parts of centella, 5-15 parts of ginkgo, 5-15 parts of folium artemisiae argyi, 5-10 parts of purslane, 5-10 parts of mangosteen, 5-10 parts of scutellaria baicalensis, 5-10 parts of moutan bark, 2-5 parts of liquorice, 2-5 parts of thyme and 1-2 parts of menthol. Completely remove garbage and toxin in the skin and quickly eliminate skin inflammation.)

1. An external preparation for preventing and treating acne through conversion of natural plant biological enzyme is characterized by comprising the following components in parts by weight: 10-20 parts of radix sophorae flavescentis, 10-20 parts of salvia miltiorrhiza, 10-20 parts of water shield, 10-20 parts of centella, 5-15 parts of ginkgo, 5-15 parts of folium artemisiae argyi, 5-10 parts of purslane, 5-10 parts of mangosteen, 5-10 parts of scutellaria baicalensis, 5-10 parts of moutan bark, 2-5 parts of liquorice, 2-5 parts of thyme and 1-2 parts of menthol.

2. The topical formulation for acne control by natural plant bio-enzymatic conversion according to claim 1, further comprising a microorganism species, wherein said microorganism species is a gram-positive species, preferably said gram-positive species comprises one of rhizopus, aspergillus niger, mucor, monascus purpureus, lactobacillus, penicillium and saccharomycetes, preferably said gram-positive species is mucor, more preferably said mucor is mucor rouxii.

3. The method for preparing the external preparation for preventing and treating acne through natural plant bio-enzyme transformation according to claim 2, is characterized by comprising the following steps:

(1) pulverizing the raw materials of claim 1, adding the pulverized raw materials into sodium dihydrogen phosphate buffer solution to obtain reaction solution, adding the microbial thallus into the reaction solution, performing enzyme conversion reaction to obtain enzyme conversion solution, and drying the enzyme conversion solution at low temperature to obtain active dry powder;

(2) mixing the active dry powder with an ethanol solution to prepare a hydroalcoholic solution, performing ultrasonic treatment at normal temperature, and performing immersion reflux circulation extraction to prepare an immersion liquid;

(3) heating the maceration extract, refluxing, immediately cooling, filtering, drying at low temperature to obtain powder, mixing the powder with water and glycerol to obtain the external preparation for preventing and treating acne by natural plant biological enzyme conversion.

4. The method for preparing the external preparation for preventing and treating acne through natural plant bio-enzyme transformation according to claim 3, wherein the method for preparing the microbial cells comprises the following steps: adopting PDA culture medium to make reproduction culture of thallus of mucor rouxii CAA09-2 at 26-30 deg.C and rotating speed of 80-100rpm, and when the thallus is in logarithmic growth phase, centrifuging to collect thallus.

5. The method for preparing an external preparation for preventing and treating acne by natural plant bio-enzyme transformation as claimed in claim 3, wherein in the step (1), the volume ratio of the raw material to the sodium dihydrogen phosphate buffer solution is 1: 8-12;

preferably, the concentration of the sodium dihydrogen phosphate buffer solution is 0.1-0.5mol/L, and the pH value is 6.0;

preferably, the mass ratio of the raw material to the microbial cells is 100:0.5-5, and preferably, the mass ratio of the raw material to the microbial cells is 100: 1-3;

preferably, the pH of the reaction solution is 5.5-6.0, the temperature of the enzyme conversion reaction is 28-40 ℃, and the time of the enzyme conversion reaction is 20-30 h.

6. The method for preparing the external preparation for preventing and treating acne through natural plant bio-enzyme transformation as claimed in claim 3, wherein in the step (2), the volume ratio of the active dry powder to the ethanol solution is 1: 10; the mass fraction of the ethanol solution is 50-80%, preferably, the mass fraction of the ethanol solution is 60-75%;

preferably, the hydroalcoholic solution is adjusted to pH 5.5-6.5 with boric acid;

preferably, the ultrasonic treatment time is 30-60 min;

preferably, the ultrasonic frequency is 20 to 40 khz;

preferably, the dipping reflux circular extraction is alternately and circularly used by a dipping extraction method and a reflux extraction method, the time of single dipping extraction or reflux extraction is not limited, the total extraction time is kept to be 3-6 hours per day, and the continuous extraction is carried out for 5-8 days;

preferably, the reflux extraction time is 3-6 h.

7. The method for preparing the external preparation for preventing and treating acne through natural plant bio-enzyme transformation as claimed in claim 3, wherein in the step (3), the steeping liquor is heated to 85-90 ℃ and is circulated for 3-6 hours under reflux.

8. The method for preparing an external preparation for preventing and treating acne through bio-enzymatic conversion of natural plants as claimed in claim 3, wherein in the step (3), the mass ratio of the powder, the glycerin and the water is 1-2:2-5: 50.

9. Use of the natural plant bio-enzyme transformed acne preventing and treating preparation prepared by the preparation method according to any one of claims 3 to 8 in preparing an external preparation for preventing and treating acne dermatosis.

Technical Field

The invention belongs to the technical field of cosmetics, and particularly relates to an external preparation for preventing and treating acne through conversion of natural plant biological enzyme, and a preparation method and application thereof.

Background

Acne is a chronic inflammatory skin disease of a pilosebaceous unit, mainly occurs in adolescents, is mainly characterized by polymorphic skin lesions of facial acne, papules, pustules, nodules and the like due to exuberant body yang heat, external gushing of blood heat and qi and blood depression, and has clinical manifestations. The surface microbiota of the acne is disordered, and the bacterial infection generates inflammation, repeatedly stimulates the hyperplasia of skin connective tissue to generate scars, so that the patients have itching and pain and suffer mental pain. A large amount of funds are invested in many countries for research, and various medicaments for treating the acne are provided, and the products have various characteristics and certain curative effects in the aspect of treating the acne. For example, hormone drugs such as diethylstilbestrol, antibiotics such as gentamicin, macrolide antibiotics, tetracycline and the like are adopted, the treatment method has certain curative effect, but the side effect is large, the cure rate is low, the disease is easy to relapse after the disease is cured, most of the drugs are chemical preparations and hormone drugs, and the side effect is easy to generate after the drug is used. There are also reports of using traditional Chinese medicine compound therapy, but the actual effect is limited, the skin availability is poor, and the relative action is slow.

In conclusion, the external preparation for preventing and treating acne through natural plant biological enzyme conversion, which can improve the bioavailability of plants and effectively improve skin microcirculation and has no toxic or side effect, needs to be researched.

Disclosure of Invention

The invention aims to provide an external preparation for preventing and treating acne through natural plant biological enzyme conversion, a preparation method and application thereof, which solve the defects of the prior art, have no scars on the skin after acne elimination, no toxic or side effect and convenient use, can effectively improve skin microcirculation, balance grease secretion, enhance cell metabolism, thoroughly eliminate garbage and toxin in the skin and quickly eliminate skin inflammation.

The invention provides an external preparation for preventing and treating acne through conversion of natural plant biological enzyme, which comprises the following components in parts by weight: 10-20 parts of radix sophorae flavescentis, 10-20 parts of salvia miltiorrhiza, 10-20 parts of water shield, 10-20 parts of centella, 5-15 parts of ginkgo, 5-15 parts of folium artemisiae argyi, 5-10 parts of purslane, 5-10 parts of mangosteen, 5-10 parts of scutellaria baicalensis, 5-10 parts of moutan bark, 2-5 parts of liquorice, 2-5 parts of thyme and 1-2 parts of menthol.

The formula achieves the advantage of combination of prevention and treatment in terms of medicament composition, and the curative effect is better than that of the formula in the prior art. The medicine for preventing and treating acne provided by the invention contains 13 traditional Chinese medicinal materials, wherein the extracted matrine and active substances have remarkable and wide antibacterial, anti-inflammatory and acaricidal effects, and also have inhibiting effects on various dermatophytes. The total tanshinone extracted from Saviae Miltiorrhizae radix has strong antibacterial effect on Staphylococcus aureus and its drug-resistant strain, and has inhibitory effect on Staphylococcus aureus, Escherichia coli, and Bacillus proteus. The water shield is sweet and cold in nature, and can clear heat, expel toxin and enhance skin immunity. The mangosteen contains various oxanone compounds, and the biological activity of the mangosteen has the effects of oxidation resistance, inflammation resistance and bacteria resistance. The thyme can diminish inflammation, sterilize, disperse blood and relieve swelling, and has good auxiliary treatment effects on various inflammations. Herba Centellae has effects of clearing away heat and toxic materials, removing dampness, relieving swelling, nourishing, relieving inflammation, and tranquilizing. Can stimulate the replacement of deep skin cells, promote the formation of collagen in the dermis and regenerate fibrin. The Scutellariae radix extract has effects in inhibiting acute inflammatory reaction, inhibiting inflammatory exudation swelling, increasing capillary permeability, and inhibiting inflammatory mediator release. Inhibiting histamine release relieves inflammatory mediators to dilate blood vessels, increases permeability of blood vessel walls, and chemotaxis of leukocytes. Has better antibacterial effect than Coptidis rhizoma, and has no drug resistance. The liquorice has the functions of clearing away heat and toxic materials, diminishing inflammation and inhibiting bacteria. The composition and the formula of the medicines are based on the principle of combining traditional Chinese medicine dialectical treatment with dialectical treatment, and are selected according to the compatibility and formula requirements of the traditional Chinese medicine aiming at the main factors causing the acne, such as endocrine factors, the action of sebum, microorganisms in hair follicles and the like, and are scientifically formulated, so that the medicine effects of the raw materials are fully exerted, and the best acne prevention and treatment effect is achieved.

The strain is a gram-positive strain, preferably, the gram-positive strain comprises one of rhizopus, aspergillus niger, mucor, monascus, lactobacillus, penicillium and saccharomycetes, preferably, the gram-positive strain is mucor, and more preferably, the mucor is mucor rouxii. Under the condition of strictly controlling enzyme digestion, the microbial strains can effectively obtain triterpenes and saponins active substances with 2-10 monosaccharide branches.

The natural plant contains a large amount of effective components, mainly comprising triterpenes, saponins, brass, alkaloids, volatile oil and polyacetylene compounds. Many triterpenes and saponins are formed by linking sapogenin and saccharide, and the saccharide composing saponin is commonly glucose, galactose, rhamnose, arabinose, xylose, glucuronic acid, galacturonic acid and the like.

Many plants contain active triterpenes and saponins, which help to soothe and calm the skin and thus prevent acne. Meanwhile, the skin can promote the regeneration of collagen of the damaged dermal layer skin, promote the blood circulation in the skin and help the color fading of the acne marks. Many plant triterpenes and saponins have relatively large sugar chains, and the size of the sugar chains affects the absorption and bioavailability of the effective substances in the skin. The invention obtains microbial strains with glucoside shear enzymes by screening, controls enzyme digestion conditions, and performs enzyme digestion of sugar branched chains of side chains by the enzymes to obtain the triterpene and saponin substances with short branched chains of 2-10 sugars. The invention finds that the application of the short-chain branch triterpenes and saponins of 2-10 sugar in the external preparation for preventing and treating acne can improve the bioavailability of skin and obtain better curative effect at lower dose.

Further, the preparation method of the external preparation for preventing and treating acne through natural plant biological enzyme conversion comprises the following steps:

(1) crushing raw materials, adding the crushed raw materials into a sodium dihydrogen phosphate buffer solution to prepare a reaction solution, then adding the microbial thalli into the reaction solution to perform an enzyme conversion reaction to prepare an enzyme conversion solution, and drying the enzyme conversion solution at a low temperature to obtain active dry powder;

(2) mixing the active dry powder with an ethanol solution to prepare a hydroalcoholic solution, performing ultrasonic treatment at normal temperature, and performing immersion reflux circulation extraction to prepare an immersion liquid;

(3) heating the maceration extract, refluxing, immediately cooling, filtering, drying at low temperature to obtain powder, mixing the powder with water and glycerol to obtain the external preparation for preventing and treating acne by natural plant biological enzyme conversion.

Further, the preparation method of the microbial thallus comprises the following steps: adopting PDA culture medium to make reproduction culture of thallus of mucor rouxii CAA09-2 at 26-30 deg.C and rotating speed of 80-100rpm, and when the thallus is in logarithmic growth phase, centrifuging to collect thallus.

Further, in the step (1), the volume ratio of the raw material to the sodium dihydrogen phosphate buffer solution is 1: 8-12;

preferably, the concentration of the sodium dihydrogen phosphate buffer solution is 0.1-0.5mol/L, and the pH value is 6.0;

preferably, the mass ratio of the raw material to the microbial cells is 100:0.5-5, and preferably, the mass ratio of the raw material to the microbial cells is 100: 1-3;

preferably, the pH of the reaction solution is 5.5-6.0, the temperature of the enzyme conversion reaction is 28-40 ℃, and the time of the enzyme conversion reaction is 20-30 h.

Further, in the step (2), the volume ratio of the active dry powder to the ethanol solution is 1: 10; the mass fraction of the ethanol solution is 50-80%, preferably, the mass fraction of the ethanol solution is 60-75%;

preferably, the hydroalcoholic solution is adjusted to pH 5.5-6.5 with boric acid;

preferably, the ultrasonic treatment time is 30-60 min;

preferably, the ultrasonic frequency is 20 to 40 khz;

preferably, the dipping reflux circular extraction is alternately and circularly used by a dipping extraction method and a reflux extraction method, the time of single dipping extraction or reflux extraction is not limited, the total extraction time is kept to be 3-6 hours per day, and the continuous extraction is carried out for 5-8 days;

preferably, the reflux extraction time is 3-6 h;

preferably, the mass fraction of the 2-10 sugar short-chain branched triterpenes and saponins in the active dry powder in the step (2) is 5-15%.

The preparation method of the invention utilizes the principle of combining ultrasonic wave and hydroalcoholic dipping method, selects the hydroalcoholic solution with strong hydrophilicity and lipophilicity, utilizes the ultrasonic wave to increase the penetration rate of effective substances, adopts the method of circularly and alternately carrying out dipping method and reflux method, comprehensively extracts the effective components, and overcomes the defects of single effective component, incomplete extracted component, unstable dosage form, toxic and side effects, inconvenient use and the like of the traditional Chinese medicine by adopting decoction, aqua and simple organic solvent to extract the traditional Chinese medicine. The invention improves the traditional process in the prior art for extracting the traditional Chinese medicine, overcomes the defects of the traditional Chinese medicine formula by using the pharmacological efficacy of the traditional Chinese medicine in the formula, reduces the medicine consumption, saves the cost and the like. The single water extraction and alcohol precipitation method and the alcohol extraction and water precipitation method are improved from the traditional Chinese medicine extraction technology, a proper water-alcohol solution is prepared to comprehensively extract the effective components, the extraction rate of the plant effective substances is increased, the stability of the medicine is enhanced, and the medicine effect is improved.

Further, in the step (3), the impregnation liquid is heated to 85-90 ℃, and reflux circulation is carried out for 3-6 h; the mass ratio of the powder, the glycerol and the water is 1-2:2-5: 50.

Furthermore, the natural plant biological enzyme conversion acne prevention external preparation is applied to the preparation of external preparations for preventing and treating acne dermatosis. The external preparation is an external cream preparation, an external mask paste or external binary spray.

Furthermore, the scheme can also prepare a paste, wherein the oil phase comprises 4-6 parts of olive oil emulsifier, 2-4 parts of shea butter, 2-4 parts of jojoba oil and 2 parts of hydroxyethyl acrylate/acryloyl dimethyl sodium taurate copolymer, and the water phase comprises 80 parts of water, 5-8 parts of glycerol, 5 parts of extracted dry powder, 2 parts of 1, 3-butanediol, 1 part of carbomer, 1 part of triethanolamine and one part of 1, 2-hexanediol. Dissolving the water phase, heating to 85 deg.C, maintaining the temperature for 30min, heating the oil phase to 85 deg.C, slowly mixing with the water phase, stirring thoroughly, emulsifying, cooling and bottling. The cream is applied to affected part 3 times daily, and 15 days is a treatment course.

The preparation method of the medicine for treating acne comprises the steps of selecting a formula, crushing, culturing thalli, converting biological enzyme, dipping, refluxing, filtering, concentrating and obtaining a dry powder finished product. Concentrating the obtained liquid at low temperature to obtain dry powder, preserving to be used as additive for preparing water aqua or cream, and adjusting the drying time at low temperature according to the prior art. The dry powder is dissolved in liquid to prepare a water aqua or paste, and the using method of the external preparation for preventing and treating the acne dermatosis comprises the following steps: when in use, the liquid medicine or the ointment applied to the affected part is uniformly applied to the acne part by hands 2-3 times a day, and 15 days is a treatment course.

The invention has the beneficial effects that:

1) the formula of the invention is scientific, and the plant bioavailability is improved through the conversion of a biological enzyme technology, so that the plant formula with higher curative effect and the functions of removing acnes, sterilizing and diminishing inflammation, controlling oil, conditioning skin and repairing scars is obtained;

2) the invention prepares the use preparation which is suitable for patients with different skin types according to the physiological characteristics of the skin, has no scars on the skin after eliminating acne, no toxic or side effect and convenient use;

3) the invention can effectively improve skin microcirculation, balance oil secretion, enhance cell metabolism, thoroughly remove garbage and toxin in the skin and quickly eliminate skin inflammation.

Detailed Description

The technical effects of the present invention will be specifically described below by way of examples. It should be noted that the following examples are given solely for the purpose of illustration and are not to be construed as limitations on the scope of the invention, as many insubstantial modifications and variations of the invention may be made by those skilled in the art without departing from the spirit and scope of the invention.

Example 1

An external preparation material for preventing and treating acne through conversion of natural plant biological enzyme comprises the following components in parts by weight: 20 parts of radix sophorae flavescentis, 15 parts of salvia miltiorrhiza, 15 parts of water shield, 10 parts of centella, 5 parts of ginkgo, 5 parts of folium artemisiae argyi, 5 parts of purslane, 5 parts of mangosteen, 5 parts of scutellaria baicalensis, 5 parts of moutan bark, 3 parts of liquorice, 2 parts of thyme and 1 part of menthol.

The preparation method comprises the following steps:

(1) adopting a PDA culture medium to carry out reproduction culture on the trichoderma rouxii CAA09-2 at the temperature of 28 ℃ and the rotating speed of 80rpm, and centrifugally collecting thalli when the thalli are in a logarithmic phase of logarithmic growth;

(2) crushing raw materials into 80-mesh powder, adding the crushed raw materials into 0.1mol/L sodium dihydrogen phosphate buffer solution (pH is 6.0) according to the volume ratio of 1:10 to prepare reaction liquid, then adding the microbial bacteria into the reaction liquid to perform enzyme conversion reaction to prepare enzyme conversion liquid, and drying the enzyme conversion liquid at low temperature to obtain active dry powder; the mass ratio of the raw materials to the microbial thallus is 100: 1; the pH value of the reaction solution is 5.5, the temperature of the enzyme conversion reaction is 28 ℃, and the time of the enzyme conversion reaction is 24 hours;

(3) mixing the active dry powder with 60% ethanol solution at a volume ratio of 1:10 to prepare aqueous alcohol solution, adjusting pH to 5.5 with boric acid, performing ultrasonic treatment at room temperature at 20 KHz for 30min, and performing immersion reflux extraction to obtain immersion liquid; the dipping reflux cyclic extraction is that the dipping extraction method and the reflux extraction method are alternately used in a cyclic manner, the time of single dipping extraction or reflux extraction is not limited, the total extraction time is kept to be 3 hours per day, and the continuous extraction is carried out for 8 days;

(4) heating the steeping liquor to 85 ℃, carrying out reflux circulation for 6h, immediately cooling and filtering, drying at low temperature to obtain powder, and mixing the powder, glycerol and water according to the mass ratio of 1:2:50 to obtain the external preparation material for preventing and treating acne through natural plant biological enzyme conversion, wherein the mass fraction of the 2-10 sugar short-chain branched-chain triterpenes and saponins in the active dry powder of the enzyme conversion product in the step (2) is 12%.

Example 2

An external preparation material for preventing and treating acne through conversion of natural plant biological enzyme comprises the following components in parts by weight: 10 parts of radix sophorae flavescentis, 10 parts of salvia miltiorrhiza, 10 parts of water shield, 15 parts of centella, 10 parts of ginkgo, 10 parts of folium artemisiae argyi, 7 parts of purslane, 7 parts of mangosteen, 7 parts of scutellaria baicalensis, 7 parts of moutan bark, 2 parts of liquorice, 3 parts of thyme and 1 part of menthol.

The preparation method comprises the following steps:

(1) adopting a PDA culture medium to carry out reproduction culture on the trichoderma rouxii CAA09-2 at the temperature of 28 ℃ and the rotating speed of 100rpm, and centrifugally collecting thalli when the thalli are in a logarithmic growth phase;

(2) crushing raw materials into 80-mesh powder, adding the crushed raw materials into 0.1mol/L sodium dihydrogen phosphate buffer solution (pH is 6.0) according to the volume ratio of 1:10 to prepare reaction liquid, then adding the microbial bacteria into the reaction liquid to perform enzyme conversion reaction to prepare enzyme conversion liquid, and drying the enzyme conversion liquid at low temperature to obtain active dry powder; the mass ratio of the raw materials to the microbial thalli is 100: 3; the pH value of the reaction solution is 6.0, the temperature of the enzyme conversion reaction is 40 ℃, and the time of the enzyme conversion reaction is 24 hours;

(3) mixing the active dry powder with 75% ethanol solution at a volume ratio of 1:10 to prepare aqueous alcohol solution, adjusting pH to 5.5 with boric acid, treating with 40 KHz ultrasonic wave at room temperature for 30min, and performing reflux extraction to obtain extractive solution; the dipping reflux cyclic extraction is that the dipping extraction method and the reflux extraction method are alternately used in a cyclic manner, the time of single dipping extraction or reflux extraction is not limited, the total extraction time is kept at 6 hours per day, and the continuous extraction is carried out for 5 days;

(4) heating the steeping liquor to 90 ℃, performing reflux circulation for 3 hours, immediately cooling and filtering, drying at low temperature to obtain powder, and mixing the powder, glycerol and water according to the mass ratio of 2:5:50 to obtain the external preparation material for preventing and treating acne through natural plant biological enzyme conversion, wherein the mass fraction of the 2-10 sugar short-chain branched-chain triterpenes and saponins in the active dry powder of the enzyme conversion product in the step (2) is 10%.

Example 3

An external preparation material for preventing and treating acne through conversion of natural plant biological enzyme comprises the following components in parts by weight: 15 parts of radix sophorae flavescentis, 20 parts of salvia miltiorrhiza, 20 parts of water shield, 20 parts of centella, 5 parts of ginkgo, 15 parts of folium artemisiae argyi, 10 parts of purslane, 10 parts of mangosteen, 10 parts of scutellaria baicalensis, 10 parts of moutan bark, 5 parts of liquorice, 5 parts of thyme and 2 parts of menthol.

The preparation method comprises the following steps:

(1) adopting a PDA culture medium to carry out reproduction culture on the trichoderma rouxii CAA09-2 at the temperature of 28 ℃ and the rotating speed of 90rpm, and centrifugally collecting thalli when the thalli are in a logarithmic phase of logarithmic growth;

(2) crushing raw materials into 80-mesh powder, adding the crushed raw materials into 0.1mol/L sodium dihydrogen phosphate buffer solution (pH is 6.0) according to the volume ratio of 1:10 to prepare reaction liquid, then adding the microbial bacteria into the reaction liquid to perform enzyme conversion reaction to prepare enzyme conversion liquid, and drying the enzyme conversion liquid at low temperature to obtain active dry powder; the mass ratio of the raw materials to the microbial thalli is 100: 2; the pH of the reaction solution is 6.0, the temperature of the enzyme conversion reaction is 34 ℃, and the time of the enzyme conversion reaction is 24 hours;

(3) mixing the active dry powder with 65% ethanol solution at a volume ratio of 1:10 to prepare aqueous alcohol solution, adjusting pH to 5.5 with boric acid, performing ultrasonic treatment at room temperature at 30 kHz for 30min, and performing immersion reflux extraction to obtain immersion liquid; the dipping reflux cyclic extraction is that the dipping extraction method and the reflux extraction method are alternately used in a cyclic manner, the time of single dipping extraction or reflux extraction is not limited, the total extraction time is kept to be 5 hours per day, and the continuous extraction is carried out for 6 days;

(4) heating the steeping liquor to 87 ℃, performing reflux circulation for 4 hours, immediately cooling and filtering, drying at low temperature to obtain powder, and mixing the powder, glycerol and water according to the mass ratio of 1:5:50 to obtain the external preparation for preventing and treating acne through natural plant biological enzyme conversion, wherein the mass fraction of the 2-10 sugar short-chain branched triterpenes and saponins in the active dry powder of the enzyme conversion substance in the step (2) is 12%.

Example 4

An external cream preparation of an external preparation material for preventing and treating acne through natural plant biological enzyme conversion is prepared by extracting dry powder, preparing a paste body like the powder prepared in the step (3) in the example 1, wherein an oil phase comprises 4-6 parts of olive oil emulsifier, 2-4 parts of shea butter, 2-4 parts of jojoba oil and 2 parts of hydroxyethyl acrylate/acryloyl dimethyl sodium taurate copolymer, and a water phase comprises 80 parts of water, 5-8 parts of glycerol, 5 parts of enzyme conversion dry powder extract, 2 parts of 1, 3-butanediol, 1 part of carbomer, 1 part of triethanolamine and one part of 1, 2-hexanediol. Dissolving the water phase, heating to 85 deg.C, holding the temperature for 30min, heating the oil phase to 85 deg.C, slowly mixing with the water phase, stirring thoroughly for emulsification, cooling and bottling. The cream is applied to affected part 3 times daily, and 14 days is a treatment course.

Example 5

An external facial mask containing natural plant biological enzyme converted topical preparation material for preventing and treating acne is provided. The dry powder was used to prepare a liquid as the powder obtained in step (3) of example 1. Comprises 85 parts of water, 0.2 part of hyaluronic acid, 3-5 parts of glycerol, 1-2 parts of allantoin, 3 parts of enzyme-converted dry powder extract, 3 parts of 1, 3-butanediol, 2 parts of 1, 2-hexanediol, 0.5 part of carbomer, 0.5 part of triethanolamine, 1 part of xanthan gum and 1 part of hydrogenated castor oil. Dissolving the dry powder in the liquid, fully emulsifying and uniformly stirring. Heating to 85 deg.C, maintaining the temperature for 30min, cooling, and packaging. The facial mask is applied to face and affected part once a day, and 14 days is a treatment course.

Example 6

A dual-component spray for external preparation of natural plant biological enzyme for preventing and treating acne is provided. The dry powder was used to prepare a liquid as the powder obtained in step (3) of example 1. Comprises 85 parts of water, 0.2 part of hyaluronic acid, 3-5 parts of glycerol, 1-2 parts of allantoin, 3 parts of enzyme-converted dry powder extract, 2 parts of 1, 3-butanediol, 2 parts of 1, 2-hexanediol and 1 part of hydrogenated castor oil. Dissolving the dry powder in the liquid, and fully and uniformly stirring. Heating to 85 deg.C, maintaining the temperature for 30min, cooling, and packaging. The spray is sprayed on the face and the affected parts of the face two to three times a day, and 14 days are a treatment course.

Clinical effect verification of related acne removing products is carried out, study objects are all patients suffering from common acne, the study objects are 60 patients, 20 men and 40 women are 20 patients, the age range of the patients is 18-30 years old, the study patients all accord with related diagnosis standards in Chinese acne treatment guidelines, and skin lesions are follicular papules, comedones, pustules, nodules, cysts and scars and are accompanied with seborrhea. Patients with allergic reactions, gestation or lactation were excluded from the study; eliminate serious dysfunction of multiple important organs of the body. The clinical effects of the patients in the group at 7d, 14d and 28d after treatment are observed, and the comprehensive evaluation is carried out by referring to Chinese acne treatment guidelines: firstly, healing: the lesions all subsided, with only minor pigmentation and mild scars; secondly, effect is displayed: the skin damage relieving degree is more than or equal to 70 percent, and various clinical symptoms are obviously improved; ③ effective: the skin damage relieving degree is more than or equal to 30 percent, and various clinical symptoms have obvious improvement trend but do not disappear; fourthly, invalidation: the skin damage relieving degree is less than 30 percent, and various clinical symptoms are not improved or have obvious aggravation trend. The statistical table 1 of the test results of the acne cream obtained by the technology of the patent is as follows:

the statistical comparison of the curative effects of the patients in the group after 7d, 14d and 28d of the application of the acne-removing cream in the treatment process:

the above experimental example was the product obtained in example 1, and the product of the comparative example was prepared in the same manner as in example 1 except that the step of converting the microbial cells into enzyme was not performed as compared to example 1, and the other components and preparation method were the same as those of example 1. From the table 1, the invention can improve the bioavailability of plants, obtain the plant formula with higher curative effect, such as acne removing, sterilization, inflammation diminishing, oil control, skin conditioning and scar repairing, has no scar on the skin after acne removing, no toxic or side effect, is convenient to use, can effectively improve skin microcirculation, balance grease secretion, enhance cell metabolism, thoroughly remove garbage and toxin in the skin, and rapidly eliminate skin inflammation; the acne removing efficiency is improved by more than 10 percent.

The above embodiments are merely preferred embodiments of the present invention, which are provided for illustrating the technical solutions of the present invention and not for limiting the same, and it should be understood by those skilled in the art that modifications or equivalent substitutions may be made to the technical solutions of the present invention without departing from the spirit and scope of the present invention, and all of them should be covered by the protection scope of the present invention.