阅读说明：本技术 一种修复女性私处微生态平衡的组合物及其制备方法和应用 (Composition for restoring microecological balance of female private parts and preparation method and application thereof ) 是由 雷登凤 胡胜兵 高合意 于 2021-01-06 设计创作，主要内容包括：本发明涉及一种修复女性私处微生态平衡的组合物及其制备方法和应用,所述修复女性私处微生态平衡的组合物的制备原料包括：五倍子、蛇床子、丹皮、苦参和植物乳杆菌发酵产物。本发明所涉及的组合物由传统中药搭配乳杆菌发酵产物组成,各中药组分从不同方面对女性私处的金黄色葡萄球菌、大肠杆菌和白色念珠菌等具有一定的抑菌能力,加入的植物乳杆菌发酵产物可有效提高私处正常微生物群落中嗜酸乳杆菌、植物乳杆菌、鼠李糖乳杆菌等菌群丰度,同时可调节阴道pH值,形成酸性屏障,进一步抑制致病菌的生长。本发明所涉及的组合物用特定的中药组分搭配乳杆菌发酵产物,五种成分科学搭配,协同增效,具有显著的修复女性私处微生态平衡的功效。(The invention relates to a composition for restoring microecological balance of female private parts, a preparation method and application thereof, wherein the preparation raw materials of the composition for restoring the microecological balance of the female private parts comprise: galla chinensis, fructus Cnidii, cortex moutan, radix Sophorae Flavescentis and Lactobacillus plantarum fermented product. The composition related by the invention is composed of traditional Chinese medicines matched with lactobacillus fermentation products, each traditional Chinese medicine component has certain bacteriostatic ability to staphylococcus aureus, escherichia coli, candida albicans and the like in female private parts from different aspects, and the added lactobacillus plantarum fermentation products can effectively improve the abundance of lactobacillus acidophilus, lactobacillus plantarum, lactobacillus rhamnosus and other flora in normal microflora in the private parts, and can adjust the pH value of vagina to form an acid barrier and further inhibit the growth of pathogenic bacteria. The composition provided by the invention uses specific traditional Chinese medicine components in combination with a lactobacillus fermentation product, the five components are scientifically matched, and the synergistic effect is achieved, so that the composition has the obvious effect of repairing the microecological balance of female private parts.)

1. A composition for restoring micro-ecological balance in a female private, the composition for restoring micro-ecological balance in a female private being prepared from the following raw materials: galla chinensis, fructus Cnidii, cortex moutan, radix Sophorae Flavescentis and Lactobacillus plantarum fermented product.

2. The composition for restoring microecological balance in female private parts according to claim 1, wherein the composition for restoring microecological balance in female private parts is prepared from the following raw materials in parts by weight: 100-150 parts of gallnut, 40-60 parts of fructus cnidii, 30-50 parts of cortex moutan, 30-50 parts of radix sophorae flavescentis and 10-50 parts of lactobacillus plantarum fermentation product.

3. A method of preparing a composition for restoring micro-ecological balance in a female human private, according to claim 1 or 2, comprising: mixing the extractive solutions of Galla chinensis, fructus Cnidii, cortex moutan and radix Sophorae Flavescentis with Lactobacillus plantarum fermented product.

4. The method for preparing a composition for restoring microecological balance in female private parts according to claim 3, wherein the method for preparing the Chinese gall extract and the kuh-seng extract comprises the following steps:

(1) grinding Galla chinensis and/or radix Sophorae Flavescentis, breaking cell wall, and sieving with 200-400 mesh sieve;

(2) soaking the sieved powder in water, reflux extracting, and filtering;

(3) pumping the filtrate into a concentration device for decompression and concentration;

(4) and standing the concentrated solution and centrifuging to obtain the Chinese gall extract and/or the radix sophorae flavescentis extract.

5. The method for preparing a composition for restoring microecological balance in female private parts according to claim 4, wherein the weight of the water of step (2) is 7-13 times the weight of Galla chinensis and/or Sophora flavescens;

preferably, the soaking time in the step (2) is 20-40 min;

preferably, the reflux extraction time of the step (2) is 2-4 h;

preferably, the concentration in the step (3) is carried out until the concentration of crude drugs is 0.2-0.4 g/g;

preferably, the standing temperature in the step (4) is 2-8 ℃, and the time is 10-12 h;

preferably, the speed of the centrifugation in the step (4) is 7000rpm, 5000-.

6. The method for preparing a composition for restoring microecological balance in female private parts according to any one of claims 3 to 5, wherein the fructus Cnidii extract and cortex moutan extract are prepared by the steps of:

(1) grinding fructus Cnidii and/or cortex moutan, breaking cell wall, and sieving with 400 mesh sieve of 200 meshes;

(2) soaking the sieved powder in ethanol water solution, then extracting under reflux, and filtering;

(3) pumping the filtrate into a concentration device for decompression and concentration;

(4) and (3) standing the concentrated solution and then centrifuging to obtain the fructus cnidii extract and/or the cortex moutan extract.

7. The method for preparing a composition for restoring microecological balance in female private parts according to claim 6, wherein the weight of the ethanol aqueous solution of step (2) is 27 to 33 times that of cnidium monnieri fruit and/or moutan cortex;

preferably, the concentration of the ethanol aqueous solution is 60-80%, preferably 70%;

preferably, the soaking time in the step (2) is 50-70 min;

preferably, the reflux extraction time of the step (2) is 3-4 h;

preferably, the concentration in the step (3) is carried out until the concentration of crude drugs is 0.05-0.15 g/g;

preferably, the speed of the centrifugation in step (4) is 7000-9000 rpm.

8. The method of preparing a composition for restoring micro-ecological balance in female private parts according to any one of claims 3 to 7, wherein the method of preparing the lactobacillus plantarum fermentation product comprises the steps of:

(S1) activating lactobacillus plantarum, inoculating the activated lactobacillus plantarum to a culture medium, and culturing to obtain a seed solution;

(S2) inoculating the seed liquid into a fermentation tank for fermentation to obtain fermentation liquid;

(S3) centrifuging the fermentation liquor, collecting the precipitate, dispersing the precipitate with pure water, adding pullulan and/or trehalose, and crushing with a high-pressure crusher to obtain the lactobacillus plantarum fermentation product.

9. The method of claim 8, wherein said fermenting of step (S2) is at a temperature of 36-38 ℃, a dissolved oxygen content of 5-20%, a pH of 6.4-6.6, and a fermentation time of 24-60 hours;

preferably, the weight ratio of the pure water to the precipitate in the step (S3) is (10-30): 1;

preferably, the weight of pullulan and/or trehalose in the step (S3) is 1-5% of the dispersion;

preferably, the crushing pressure in the step (S3) is 120-180MPa, the temperature is 2-6 ℃, and the crushing times are 1-3.

10. Use of a composition for restoring micro-ecological balance in a female human being according to claim 1 or 2, in the manufacture of a care product for restoring micro-ecological balance in a female human being.

Technical Field

The invention belongs to the technical field of female care products, and particularly relates to a composition for restoring microecological balance of female private parts, and a preparation method and application thereof.

Background

Along with sudden and violent career advancement, the pressure is simultaneously pressed on the physiology and the psychology of modern women. The occurrence of stress directly causes the reduction of human immunity, and in addition, the modern living environment is severe day by day, and gynecological diseases are developed in an ascending trend. Traditional Chinese medicine extracts, antibiotics and other chemical bactericides are usually added into the existing commercial private care products, and although the products have high bactericidal rate and can kill a large amount of female vaginal pathogenic flora in a short period, the products can kill vaginal resident bacteria and probiotic flora simultaneously, so that the micro-ecological balance of the private is damaged, and the resistance of the private to pathogenic bacteria can be reduced after long-term use.

CN112022745A discloses a nutrient solution with functions of bacteriostasis, disinfection, skin cleaning and moistening and micro-ecological balance keeping, which is prepared by the following weight percentages: 0.1-0.5% of polyaminopropyl biguanide, 0.1-0.4% of benzalkonium chloride, 0.1-0.3% of phenoxyethanol, 0.05-0.1% of chlorphenesin, 0.01-0.05% of ethylhexyl glycerol, 0.01-0.05% of menthol, 0.01-0.05% of borneol, 0.01-0.04% of sodium citrate, 0.01-0.04% of citric acid, 0.01-0.02% of vitamin E, 0.001-0.002% of rose essential oil, 1-2% of prebiotics and the balance of water. The product has strong effect in killing Candida albicans which is frequently causing gynecological diseases, and effectively maintains the healthy environment of private microorganisms.

CN104721668A discloses a private part care solution, which consists of the following substances: 5.62 percent of polyporus umbellatus, 11.24 percent of rhizoma atractylodis, 11.24 percent of radix stemonae, 8.99 percent of fructus cnidii, 5.62 percent of rhizoma smilacis glabrae, 5.62 percent of radix sophorae flavescentis, 11.24 percent of dogwood, 11.24 percent of chrysanthemum, 11.24 percent of pomegranate rind, 11.24 percent of radix aristolochiae, 5.62 percent of alum, 0.56 percent of apple essence and 0.56 percent of benzoic acid. Through detection, the private part care solution has strong killing capability on diplococcus gonorrhoeae, staphylococcus aureus, escherichia coli and herpes virus.

Therefore, it is very significant to develop a product which has strong sterilization capability, destroys the micro-ecological balance of the private parts and restores the micro-ecological balance of the female private parts.

Disclosure of Invention

Aiming at the defects of the prior art, the invention aims to provide a composition for restoring microecological balance of female private parts, a preparation method and application thereof.

In order to achieve the purpose, the invention adopts the following technical scheme:

in a first aspect, the present invention provides a composition for restoring microecological balance in a female private part, which is prepared from the following raw materials: galla chinensis, fructus Cnidii, cortex moutan, radix Sophorae Flavescentis and Lactobacillus plantarum fermented product.

The composition related by the invention is composed of traditional Chinese medicines matched with lactobacillus fermentation products, and each traditional Chinese medicine component has certain bacteriostatic ability to staphylococcus aureus, escherichia coli, candida albicans and the like at female private parts from different aspects, so that the problem that the stimulation to vaginal mucosa is possibly caused by adding chemical bacteriostatic agents such as chlorhexidine acetate or ethylparaben and the like into the traditional private parts products is solved; the gallnut is rich in hydrolyzed tannic acid, can effectively destroy membrane protein of pathogenic microorganisms in vagina, enables cytoplasm to flow outwards and kills the pathogenic microorganisms, and meanwhile, the tannic acid has a astringing effect and can tighten the skin of private parts and enable the private parts to return to a normal state; fructus Cnidii is rich in osthole, and has antiallergic, analgesic and antiinflammatory effects; the radix sophorae flavescentis is rich in matrine which can promote the release of anti-inflammatory factors, so that the effects of immunosuppression, antianaphylaxis and anti-inflammation are achieved; the cortex moutan contains paeonol, is an active monomer component extracted from root bark of Chinese medicinal peony, and has antipyretic, antiinflammatory, and antibacterial effects.

The added lactobacillus plantarum fermentation product can effectively improve the abundance of flora such as lactobacillus acidophilus, lactobacillus plantarum, lactobacillus rhamnosus and the like in the normal microbial community at the private part, solves the problem that the ecological balance of the resident flora at the private part is damaged by the broad-spectrum bactericidal property of the traditional personal care product, can adjust the pH value of the vagina, forms an acid barrier and further inhibits the growth of pathogenic bacteria. The composition provided by the invention uses specific traditional Chinese medicine components in combination with a lactobacillus fermentation product, the five components are scientifically matched, and the synergistic effect is achieved, so that the composition has the obvious effect of repairing the microecological balance of female private parts.

Preferably, the composition for restoring the micro-ecological balance of the female private parts is prepared from the following raw materials in parts by weight: 100-150 parts of gallnut, 40-60 parts of fructus cnidii, 30-50 parts of cortex moutan, 30-50 parts of radix sophorae flavescentis and 10-50 parts of lactobacillus plantarum fermentation product.

When the preparation raw materials in the composition are combined and matched according to the specific mass ratio, the composition plays a more remarkable role in repairing the microecological balance of female private parts.

The weight parts of the gallnut can be 100 parts, 105 parts, 110 parts, 115 parts, 120 parts, 125 parts, 130 parts, 135 parts, 140 parts, 145 parts, 150 parts and the like.

The fructus cnidii can be 40 parts, 45 parts, 50 parts, 55 parts, 60 parts and the like in parts by weight.

The cortex moutan can be 30 parts, 35 parts, 40 parts, 45 parts, 50 parts and the like.

The weight parts of the sophora flavescens can be 30 parts, 35 parts, 40 parts, 45 parts, 50 parts and the like.

The lactobacillus plantarum fermentation product can be 10 parts, 15 parts, 20 parts, 25 parts, 30 parts, 35 parts, 40 parts, 45 parts, 50 parts and the like in parts by weight.

Other specific point values within the above numerical ranges can be selected, and are not described in detail herein.

In a second aspect, the present invention provides a method of preparing a composition for restoring micro-ecological balance in a female human private, the method comprising: mixing the extractive solutions of Galla chinensis, fructus Cnidii, cortex moutan and radix Sophorae Flavescentis with Lactobacillus plantarum fermented product.

The preparation method of the composition for restoring the microecological balance of the female private parts, which is provided by the invention, is simple and easy to operate, is very suitable for large-scale industrial production, and has obvious practicability and practical significance.

Because the Chinese medicines of the Chinese gall, the common cnidium fruit, the cortex moutan and the lightyellow sophora root are different in specificity, the invention develops a specific Chinese medicine extraction mode aiming at different Chinese medicines, so that the Chinese gall, the common cnidium fruit, the cortex moutan and the lightyellow sophora root can be matched with other components to exert more remarkable beneficial effects as described above. The method comprises the following specific steps:

in the invention, the preparation method of the gallnut extract and the sophora flavescens extract comprises the following steps:

(1) grinding Galla chinensis and/or radix Sophorae Flavescentis, breaking cell wall, and sieving with 200-400 mesh sieve;

(2) soaking the sieved powder in water, reflux extracting, and filtering;

(3) pumping the filtrate into a concentration device for decompression and concentration;

(4) and standing the concentrated solution and centrifuging to obtain the Chinese gall extract and/or the radix sophorae flavescentis extract.

Preferably, the weight of the water in the step (2) is 7-13 times of the weight of the gallnut and/or the sophora flavescens, for example, 7 times, 8 times, 9 times, 10 times, 11 times, 12 times or 13 times, and other specific values in the numerical range can be selected, and are not repeated herein.

Preferably, the soaking time in step (2) is 20-40min, for example, 20min, 25min, 30min, 35min or 40min, and other specific values in the value range can be selected, and are not described herein again.

Preferably, the reflux extraction time in step (2) is 2-4h, for example, 2h, 2.5h, 3h, 3.5h, 4h, etc., and other specific values in the value range can be selected, which is not described herein again.

Preferably, the concentration in step (3) is carried out until the concentration of the crude drug is 0.2-0.4g/g, such as 0.2g/g, 0.25g/g, 0.3g/g, 0.35g/g, 0.4g/g, etc.

Preferably, the temperature of the standing in step (4) is 2-8 deg.C, such as 2 deg.C, 3 deg.C, 4 deg.C, 5 deg.C, 6 deg.C, 7 deg.C, 8 deg.C, etc., and the time is 10-12h, such as 10h, 10.5h, 11h, 11.5h, 12h, etc. Other specific point values in the numerical range can be selected, and are not described in detail herein.

Preferably, the centrifugation speed in step (4) is 5000-7000rpm, such as 5000rpm, 5500rpm, 6000rpm, 6500rpm, 7000rpm, etc., and other specific values in the numerical range can be selected, which is not described in detail herein.

In the invention, the preparation method of the fructus cnidii extracting solution and the cortex moutan extracting solution comprises the following steps:

(1) grinding fructus Cnidii and/or cortex moutan, breaking cell wall, and sieving with 400 mesh sieve of 200 meshes;

(2) soaking the sieved powder in ethanol water solution, then extracting under reflux, and filtering;

(3) pumping the filtrate into a concentration device for decompression and concentration;

(4) and (3) standing the concentrated solution and then centrifuging to obtain the fructus cnidii extract and/or the cortex moutan extract.

Preferably, the weight of the ethanol aqueous solution in the step (2) is 27-33 times of the weight of the fructus cnidii and/or the cortex moutan, for example, 27 times, 28 times, 29 times, 30 times, 31 times, 32 times or 33 times, and the like, and other specific values in the value range can be selected, and are not repeated herein.

Preferably, the concentration of the ethanol aqueous solution is 60-80%, such as 60%, 65%, 70%, 75%, 80%, etc., and other specific values within the numerical range can be selected, which is not described in detail herein. Preferably 70%.

Preferably, the soaking time in step (2) is 50-70min, for example, 50min, 55min, 60min, 65min or 70min, and other specific values in the value range can be selected, and are not repeated herein.

Preferably, the reflux extraction time in step (2) is 3-4h, for example, 3h, 3.5h, 4h, etc., and other specific point values within the range of values can be selected, which is not described herein again.

Preferably, the concentration in step (3) is carried out until the concentration of the crude drug is 0.05-0.15g/g, such as 0.05g/g, 0.08g/g, 0.10g/g, 0.12g/g, 0.15g/g, etc.

Preferably, the centrifugation speed in step (4) is 7000-9000rpm, such as 7000rpm, 7500rpm, 8000rpm, 8500rpm, 9000rpm, etc., and other specific values within the numerical range can be selected, which is not described herein again.

The lactobacillus plantarum fermentation product related by the invention is prepared by adopting a specific preparation method, and all conditions are matched with each other and mutually influenced, so that the lactobacillus plantarum fermentation product can play more excellent beneficial effects when being matched with traditional Chinese medicine components. The method comprises the following specific steps:

in the invention, the preparation method of the lactobacillus plantarum fermentation product comprises the following steps:

(S1) activating lactobacillus plantarum, inoculating the activated lactobacillus plantarum to a culture medium, and culturing to obtain a seed solution;

(S2) inoculating the seed liquid into a fermentation tank for fermentation to obtain fermentation liquid;

(S3) centrifuging the fermentation liquor, collecting the precipitate, dispersing the precipitate with pure water, adding pullulan and/or trehalose, and crushing with a high-pressure crusher to obtain the lactobacillus plantarum fermentation product.

Preferably, the temperature of the fermentation in the step (S2) is 36-38 ℃, such as 36 ℃, 37 ℃, 38 ℃ and the like; dissolved oxygen amount is 5-20%, for example, 5%, 10%, 15%, 20%, etc.; controlling the pH value to be 6.4-6.6, such as 6.4, 6.5, 6.6 and the like; the fermentation time is 24-60h, such as 24h, 36h, 48h, 60h, etc. Other specific point values within the above numerical range can be selected, and are not described in detail herein.

Preferably, the weight ratio of the pure water to the precipitate in the step (S3) is (10-30):1, for example, 10:1, 15:1, 20:1, 25:1, 30:1, etc., and other specific values in the numerical range can be selected, which is not described in detail herein.

Preferably, the weight of pullulan and/or trehalose in the step (S3) is 1-5% of the dispersion, such as 1%, 2%, 3%, 4%, 5%, etc., and other specific values in the value range can be selected, which is not described in detail herein.

Preferably, the pressure for the crushing in the step (S3) is 120-180MPa, such as 120MPa, 130MPa, 140MPa, 150MPa, 160MPa, 170MPa, 180MPa, etc.; the temperature is 2-6 deg.C, such as 2 deg.C, 3 deg.C, 4 deg.C, 5 deg.C, 6 deg.C, etc.; the number of crushing times is 1-3, such as 1,2, 3. Other specific point values within the above numerical range can be selected, and are not described in detail herein.

In a third aspect, the present invention provides the use of a composition for restoring microecological balance in a female human being, as defined in the first aspect, in the manufacture of a care product for restoring microecological balance in a female human being.

Compared with the prior art, the invention has the following beneficial effects:

the composition related by the invention is composed of traditional Chinese medicines matched with lactobacillus fermentation products, and each traditional Chinese medicine component has certain bacteriostatic ability to staphylococcus aureus, escherichia coli, candida albicans and the like at female private parts from different aspects, so that the problem that the stimulation to vaginal mucosa is possibly caused by adding chemical bacteriostatic agents such as chlorhexidine acetate or ethylparaben and the like into the traditional private parts products is solved; the added lactobacillus plantarum fermentation product can effectively improve the abundance of flora such as lactobacillus acidophilus, lactobacillus plantarum, lactobacillus rhamnosus and the like in the normal microbial community at the private part, solves the problem that the ecological balance of the resident flora at the private part is damaged by the broad-spectrum bactericidal property of the traditional personal care product, can adjust the pH value of the vagina, forms an acid barrier and further inhibits the growth of pathogenic bacteria. The composition provided by the invention uses specific traditional Chinese medicine components in combination with a lactobacillus fermentation product, the five components are scientifically matched, and the synergistic effect is achieved, so that the composition has the obvious effect of repairing the microecological balance of female private parts.

Detailed Description

To further illustrate the technical means and effects of the present invention, the following further describes the technical solution of the present invention with reference to the preferred embodiments of the present invention, but the present invention is not limited to the scope of the embodiments.

The following examples refer to Lactobacillus plantarum having ATCC number: ATCC 8014.

The ATCC number of E.coli is: ATCC 25922.

The ATCC nos. of pseudomonas aeruginosa are: ATCC 9027.

The ATCC nos. for staphylococcus aureus are: ATCC 6538.

The ATCC nos. of candida albicans are: ATCC 10231.

The lactobacillus acidophilus ATCC nos: ATCC 4356.

Example 1

The embodiment provides a composition for restoring microecological balance of female private parts, which comprises the following raw materials in parts by weight: 120 parts of nutgall, 50 parts of common cnidium fruit, 40 parts of cortex moutan, 45 parts of radix sophorae flavescentis and 30 parts of lactobacillus plantarum fermentation product. The preparation method comprises the following steps:

(1) grinding Galla chinensis and radix Sophorae Flavescentis, breaking cell wall, and sieving with 300 mesh sieve; soaking the sieved powder in 10 times of water for 30min, reflux extracting for 3h, and filtering; pumping the filtrate into a concentration device, and concentrating under reduced pressure until the concentration of crude drug is 0.3 g/g; standing the concentrated solution at 4 deg.C for 11 hr, and centrifuging at 6000rpm to obtain Galla chinensis and radix Sophorae Flavescentis extractive solution.

(2) Grinding fructus Cnidii and/or cortex moutan, breaking cell wall, and sieving with 300 mesh sieve; soaking the sieved powder in 30 times of 70% ethanol water solution for 60min, reflux extracting for 4h, and filtering; pumping the filtrate into a concentration device, and concentrating under reduced pressure until the concentration of crude drug is 0.1 g/g; standing the concentrated solution, and centrifuging at 8000rpm to obtain fructus Cnidii and cortex moutan extractive solution.

(3) Inoculating lactobacillus plantarum stored at 4 ℃ into an MRS liquid culture medium for activation, and culturing for 24h at 37 ℃ for 2 times; inoculating the activated strain into an MRS liquid test tube culture medium, and culturing at 37 ℃ for 24h to obtain a seed solution;

(4) inoculating the seed solution into a fermentation tank according to the inoculation amount of 5%, wherein the fermentation temperature is 37 ℃, the dissolved oxygen amount is 15%, the pH value is controlled at 6.5, and the fermentation time is 48h, so as to obtain fermentation liquor;

(5) separating the fermentation liquor in a high-speed centrifugation mode, and collecting thallus precipitates; washing the precipitate with pure water for 3 times, dispersing the precipitate with pure water according to the feed-liquid ratio of 1:20, and adding 4% pullulan as a protective agent. Crushing the liquid at low temperature with a high-pressure crusher at 150MPa and 4 deg.C for 2 times to obtain Lactobacillus plantarum fermented product.

(6) Mixing the extractive solutions of Galla chinensis, fructus Cnidii, cortex moutan and radix Sophorae Flavescentis with Lactobacillus plantarum fermented product.

Example 2

The embodiment provides a composition for restoring microecological balance of female private parts, which comprises the following raw materials in parts by weight: 110 parts of gallnut, 60 parts of common cnidium fruit, 45 parts of cortex moutan, 50 parts of radix sophorae flavescentis and 20 parts of lactobacillus plantarum fermentation product. The preparation method comprises the following steps:

(1) grinding Galla chinensis and radix Sophorae Flavescentis, breaking cell wall, and sieving with 250 mesh sieve; soaking the sieved powder in 8 times of water for 40min, reflux extracting for 2h, and filtering; pumping the filtrate into a concentration device, and concentrating under reduced pressure until the concentration of crude drug is 0.3 g/g; standing the concentrated solution at 4 deg.C for 12 hr, and centrifuging at 6000rpm to obtain Galla chinensis and radix Sophorae Flavescentis extractive solution.

(2) Grinding fructus Cnidii and/or cortex moutan, breaking cell wall, and sieving with 250 mesh sieve; soaking the sieved powder in 27 times of 70% ethanol water solution for 70min, reflux extracting for 3h, and filtering; pumping the filtrate into a concentration device, and concentrating under reduced pressure until the concentration of crude drug is 0.1 g/g; standing the concentrated solution, and centrifuging at 8000rpm to obtain fructus Cnidii and cortex moutan extractive solution.

(3) Inoculating lactobacillus plantarum stored at 4 ℃ into an MRS liquid culture medium for activation, and culturing for 24h at 37 ℃ for 2 times; inoculating the activated strain into an MRS liquid test tube culture medium, and culturing at 37 ℃ for 24h to obtain a seed solution;

(4) inoculating the seed solution into a fermentation tank according to the inoculation amount of 5%, wherein the fermentation temperature is 37 ℃, the dissolved oxygen amount is 10%, the pH value is controlled at 6.4, and the fermentation time is 60 hours, so as to obtain fermentation liquor;

(5) separating the fermentation liquor in a high-speed centrifugation mode, and collecting thallus precipitates; washing the precipitate with pure water for 3 times, dispersing the precipitate with pure water according to the feed-liquid ratio of 1:20, and adding 4% trehalose as protectant. Crushing the liquid at low temperature with a high-pressure crusher at 130MPa and 4 deg.C for 3 times to obtain Lactobacillus plantarum fermented product.

(6) Mixing the extractive solutions of Galla chinensis, fructus Cnidii, cortex moutan and radix Sophorae Flavescentis with Lactobacillus plantarum fermented product.

Example 3

The embodiment provides a composition for restoring microecological balance of female private parts, which comprises the following raw materials in parts by weight: 140 parts of gallnut, 40 parts of common cnidium fruit, 35 parts of cortex moutan, 30 parts of radix sophorae flavescentis and 40 parts of lactobacillus plantarum fermentation product. The preparation method comprises the following steps:

(1) grinding Galla chinensis and radix Sophorae Flavescentis, breaking cell wall, and sieving with 400 mesh sieve; soaking the sieved powder in 12 times of water for 40min, reflux extracting for 4h, and filtering; pumping the filtrate into a concentration device, and concentrating under reduced pressure until the concentration of crude drug is 0.3 g/g; standing the concentrated solution at 4 deg.C for 10 hr, and centrifuging at 6000rpm to obtain Galla chinensis and radix Sophorae Flavescentis extractive solution.

(2) Grinding fructus Cnidii and cortex moutan, breaking cell wall, and sieving with 400 mesh sieve; soaking the sieved powder in 33 times of 70% ethanol water solution for 70min, reflux extracting for 3h, and filtering; pumping the filtrate into a concentration device, and concentrating under reduced pressure until the concentration of crude drug is 0.1 g/g; standing the concentrated solution, and centrifuging at 8000rpm to obtain fructus Cnidii and cortex moutan extractive solution.

(3) Inoculating lactobacillus plantarum stored at 4 ℃ into an MRS liquid culture medium for activation, and culturing for 24h at 37 ℃ for 2 times; inoculating the activated strain into an MRS liquid test tube culture medium, and culturing at 37 ℃ for 24h to obtain a seed solution;

(4) inoculating the seed solution into a fermentation tank according to the inoculation amount of 5%, wherein the fermentation temperature is 37 ℃, the dissolved oxygen amount is 20%, the pH value is controlled at 6.6, and the fermentation time is 24 hours, so as to obtain a fermentation liquid;

(5) separating the fermentation liquor in a high-speed centrifugation mode, and collecting thallus precipitates; washing the precipitate with pure water for 3 times, dispersing the precipitate with pure water according to the feed-liquid ratio of 1:30, and adding 5% pullulan as a protective agent. Crushing the liquid at low temperature with a high-pressure crusher at 170MPa and 4 deg.C for 1 time to obtain Lactobacillus plantarum fermented product.

(6) Mixing the extractive solutions of Galla chinensis, fructus Cnidii, cortex moutan and radix Sophorae Flavescentis with Lactobacillus plantarum fermented product.

Example 4

This example provides a composition for restoring microecological balance in female private parts, which is prepared from the same raw materials as in example 1, except that the fructus Cnidii and cortex moutan are extracted with 60% ethanol in step (2) of the preparation method, and other conditions are kept unchanged.

Example 5

This example provides a composition for restoring microecological balance in female private parts, which was prepared from the following raw materials: 90 parts of gallnut, 60 parts of common cnidium fruit, 50 parts of cortex moutan, 55 parts of radix sophorae flavescentis and 30 parts of lactobacillus plantarum fermentation product. The preparation method is the same as that of example 1.

Example 6

This example provides a composition for restoring microecological balance in female private parts, which was prepared from the following raw materials: 160 parts of nutgall, 40 parts of common cnidium fruit, 30 parts of cortex moutan, 25 parts of radix sophorae flavescentis and 30 parts of lactobacillus plantarum fermentation product. The preparation method is the same as that of example 1.

Example 7

This example provides a composition for restoring microecological balance in female private parts, which was prepared from the following raw materials: 130 parts of gallnut, 60 parts of common cnidium fruit, 45 parts of cortex moutan, 50 parts of radix sophorae flavescentis and 60 parts of lactobacillus plantarum fermentation product. The preparation method is the same as that of example 1.

Comparative example 1

This comparative example provides a composition which differs from example 1 in the starting materials for its preparation: 90 parts of fructus cnidii, 80 parts of cortex moutan, 85 parts of radix sophorae flavescentis and 30 parts of lactobacillus plantarum fermentation product. The preparation method is the same as that of example 1.

Comparative example 2

This comparative example provides a composition which differs from example 1 in the starting materials for its preparation: 150 parts of Chinese gall, 50 parts of cortex moutan, 55 parts of radix sophorae flavescentis and 30 parts of lactobacillus plantarum fermentation products. The preparation method is the same as that of example 1.

Comparative example 3

This comparative example provides a composition which differs from example 1 in the starting materials for its preparation: 150 parts of Chinese gall, 55 parts of common cnidium fruit, 50 parts of lightyellow sophora root and 30 parts of lactobacillus plantarum fermentation product. The preparation method is the same as that of example 1.

Comparative example 4

This comparative example provides a composition which differs from example 1 in the starting materials for its preparation: 150 parts of Chinese gall, 60 parts of common cnidium fruit, 45 parts of cortex moutan and 30 parts of lactobacillus plantarum fermentation product. The preparation method is the same as that of example 1.

Comparative example 5

This comparative example provides a composition which differs from example 1 in the starting materials for its preparation: contains no lactobacillus plantarum fermentation product component, and other raw materials and contents are kept unchanged. The preparation process is as in example 1.

Test example 1

The test examples carried out a 24-hour bacteriostatic test on the compositions prepared in examples 1-7 and comparative examples 1-5, and characterized the inhibitory effect of each composition on pathogenic bacteria in private parts. The test method is specifically referred to "Disinfection Specification" (2002 edition).

The results are shown in table 1:

TABLE 1

Test example 2

Escherichia coli, Lactobacillus plantarum and Lactobacillus acidophilus were inoculated into an agar nutrient medium containing 5% of the composition prepared in examples 1 to 7 and comparative examples 1 to 5 (see "Disinfection Specification" (2002) for formulation) at a viable count ratio of 1:1:1, and after 24 hours of culture according to a standard method, the number of colonies of each bacterial population was counted. The pharmacological effects of the composition on up-regulation of resident flora and down-regulation of pathogenic flora are demonstrated by co-culturing the resident flora and pathogenic bacteria causing the destruction of microecological balance in the same environment, simulating the actual situation of the colonies in the private area under the condition of administration of patients and changing the abundance of different floras.

The results are shown in table 2:

TABLE 2

Test example 3

(1) Preparing a private part care solution, wherein the specific formula is as follows:

phase A: 80 parts of deionized water; 0.2 part of sodium hyaluronate; 0.2 part of acrylate/C10-30 alkanol acrylate cross-linked polymer; 5 parts of glycerol; 2 parts of 1, 3-propylene glycol;

phase B: 2 parts of 1, 2-pentanediol; 0.2 part of ethylhexyl glycerin; 0.1 part of aminomethyl propanol; citric acid monohydrate 0.09 part; example 1 preparation of 10 parts of a microecologically balanced composition;

and C phase: 260.2 parts of PEG; 0.002 part of pelargonium roseum oil; 0.003 part of cananga oil; 0.005 part of bergamot fruit extract;

the preparation method comprises the following steps:

and adding the sodium hyaluronate in the phase A into glycerol for dispersion, dispersing the acrylate/C10-30 alkanol acrylate cross-linked polymer by using water, adding other components for mixing, mixing the A, B, C phases, and stirring uniformly to obtain the private care gel for human clinical experiments.

(2) Test method

51 patients diagnosed with vaginal pathogenic microorganism infection accompanied with pudendum pruritus and increased vaginal secretion with mucopurulent, dark complexion and withered yellow are divided into A, B, C groups, each group comprises 17 patients, and the group A uses the private microecological nursing liquid twice a day, 5mL each time and lasts for two weeks; b, using a conventional nursing solution sold in the market twice a day, 5mL each time, and lasting for two weeks; group C only performed a simple two week daily nursing rinse. Microbial detection of vaginal secretion is carried out before and after use, and compared with healthy people, the influence of the private part treatment gel on the female private part micro-ecology is analyzed from three aspects of flora concentration, dominant bacteria, fungal infection and the like. The vaginal secretion microorganism detection results of healthy women are shown in the following table:

(3) results of the experiment

The results of the microbial testing of vaginal secretions before and after use of the product by subjects are shown in table 3:

TABLE 3

As can be seen from Table 3: after the group A uses the private microecological care solution, the germ flora density of the reproductive system of a patient is reduced, the diversity of the germ flora is reduced, beneficial bacteria dominate, and no fungal infection exists; after the group B uses the conventional nursing liquid sold in the market, the germ density of the reproductive system of a patient is reduced, the diversity of the florae is still high, and a large number of pathogenic bacteria still exist and the florae are abnormal; group C did not use the nursing and repairing product, and the reproductive system microecology was abnormal, and the symptoms were not improved.

The composition for repairing the micro-ecological balance of the female private parts is formed by combining traditional Chinese medicines and lactobacillus plantarum fermentation products, traditional Chinese medicine extracts can effectively reduce the number of pathogenic bacteria, the lactobacillus plantarum fermentation products contained in the formula have a promoting effect on the growth of the lactobacillus in the private ecological system, and the lactobacillus can inhibit the growth of the pathogenic bacteria through competitive inhibition among microbial communities, so that the resistance of the vagina to the pathogenic bacteria is enhanced, and a good long-acting maintenance effect is achieved.

The applicant states that the present invention is illustrated by the above examples to a composition for restoring micro-ecological balance in female private parts, a method for preparing the same and applications thereof, but the present invention is not limited to the above examples, i.e. it does not mean that the present invention must be implemented by means of the above examples. It should be understood by those skilled in the art that any modification of the present invention, equivalent substitutions of the raw materials of the product of the present invention, addition of auxiliary components, selection of specific modes, etc., are within the scope and disclosure of the present invention.

The preferred embodiments of the present invention have been described in detail, however, the present invention is not limited to the specific details of the above embodiments, and various simple modifications may be made to the technical solution of the present invention within the technical idea of the present invention, and these simple modifications are within the protective scope of the present invention.

It should be noted that the various technical features described in the above embodiments can be combined in any suitable manner without contradiction, and the invention is not described in any way for the possible combinations in order to avoid unnecessary repetition.