阅读说明：本技术 一种肉苁蓉中草药酵素及其制备方法 (Cistanche Chinese herbal medicine enzyme and preparation method thereof ) 是由 王磊 于 2020-12-16 设计创作，主要内容包括：本发明涉及一种肉苁蓉中草药酵素及其制备方法,肉苁蓉中草药酵素以肉苁蓉、杜仲雄花、南瓜籽、黄精、黑果枸杞、玛咖粉、金银花、蒲公英、鱼腥草、马齿苋、茯苓、芡实、赤小豆、益智仁、淡竹叶、薏苡仁、覆盆子为主要原料药,在制备过程中经提取、浓缩、发酵等工艺加工成酵素。本发明的酵素保留原有活性物质,提高了原有植物的功效,以达到温补肾阳、增强免疫力、提升男性功能的效果。(The invention relates to a cistanche Chinese herbal medicine enzyme and a preparation method thereof, the cistanche Chinese herbal medicine enzyme takes cistanche, eucommia male flower, pumpkin seed, rhizoma polygonati, black fruit medlar, maca powder, honeysuckle, dandelion, houttuynia cordata, purslane, tuckahoe, gorgon fruit, red bean, sharpleaf galangal fruit, lophatherum gracile, coix seed and raspberry as main raw material medicines, and is processed into the enzyme through the processes of extraction, concentration, fermentation and the like in the preparation process. The enzyme disclosed by the invention retains the original active substances, improves the efficacy of the original plants, and achieves the effects of warming and recuperating kidney yang, enhancing the immunity and improving the male functions.)

1. The cistanche Chinese herbal medicine enzyme is characterized by being prepared from the following raw materials: cistanche, eucommia male flower, pumpkin seed, rhizoma polygonati, lycium ruthenicum, maca powder, honeysuckle, dandelion, houttuynia, purslane, poria cocos, gordon euryale seed, phaseolus calcaratus, fructus alpiniae oxyphyllae, lophatherum gracile, coix seed, raspberry and microbial inoculum.

2. The cistanche salsa Chinese herbal medicine enzyme according to claim 1, wherein the cistanche salsa Chinese herbal medicine enzyme is prepared from the following raw materials in parts by weight:

40-70 parts of cistanche, 10-20 parts of eucommia male flowers, 10-20 parts of pumpkin seeds, 20-30 parts of rhizoma polygonati, 10-20 parts of lycium ruthenicum, 30-50 parts of maca powder, 5-10 parts of honeysuckle, 5-10 parts of dandelion, 5-10 parts of houttuynia cordata, 5-10 parts of purslane, 5-10 parts of poria cocos, 5-10 parts of gordon euryale seeds, 5-10 parts of phaseolus calcaratus, 5-10 parts of sharpleaf galangal fruit, 5-10 parts of lophatherum gracile, 5-10 parts of coix seeds and 5-10 parts of raspberry.

3. The cistanche salsa Chinese herbal medicine enzyme according to claim 2, wherein the cistanche salsa Chinese herbal medicine enzyme is prepared from the following raw materials in parts by weight:

55 parts of cistanche, 15 parts of eucommia male flower, 15 parts of pumpkin seed, 25 parts of rhizoma polygonati, 15 parts of lycium ruthenicum, 40 parts of maca powder, 8 parts of honeysuckle, 8 parts of dandelion, 8 parts of houttuynia cordata, 8 parts of purslane, 7.5 parts of poria cocos, 7.5 parts of gordon euryale seed, 7.5 parts of phaseolus calcaratus, 7 parts of fructus alpiniae oxyphyllae, 7 parts of lophatherum gracile, 7 parts of semen coicis and 7 parts of raspberry.

4. The cistanche salsa herbal ferment of any one of claims 1-3, wherein the microbial agent comprises one or a combination of at least two of Bifidobacterium, Lactobacillus, Streptococcus, lactococcus, Propionibacterium, Leuconostoc, Kluyveromyces, Pediococcus, Staphylococcus, and Bacillus.

5. The method for preparing cistanche tubulosa Chinese herbal medicine enzyme according to any one of claims 1 to 4, wherein the method for preparing cistanche tubulosa Chinese herbal medicine enzyme comprises the following steps:

(1) cleaning Cistanchis herba, Eucommiae cortex male flower, semen Cucurbitae, rhizoma Polygonati, fructus Lycii, maca powder, flos Lonicerae, herba Taraxaci, herba Houttuyniae, herba Portulacae, Poria, semen euryales, semen Phaseoli, fructus Alpinae Oxyphyllae, folium Bambusae, Coicis semen and Rubi fructus, respectively crushing, sieving, mixing to obtain mixture;

(2) putting the mixture obtained in the step (1) into an extraction tank, adding water, extracting twice, and combining filtrates to form an extracting solution;

(3) and (3) adding golden orange juice, fig juice, orange juice and lemon juice into the extracting solution obtained in the step (2), uniformly mixing, adding a microbial agent, fermenting for 50-75h to obtain a fermentation liquid, filtering, distilling the filtered fermentation liquid at a low temperature to remove alcohol and methanol, sterilizing and homogenizing to obtain the cistanche Chinese herbal medicine enzyme.

6. The method for preparing cistanche tubulosa Chinese herbal medicine ferment as claimed in claim 5, wherein in the step (1), the sieving is 80 mesh sieving.

7. The method for preparing cistanche tubulosa-Chinese herbal medicine enzyme according to claim 5, wherein in the step (2), the extraction is specifically as follows: adding water which is 10-20 times of the weight of the mixture into the mixture to immerse all the raw materials in the water, heating the mixture to 55-65 ℃, preserving the heat, soaking the mixture for 6 hours, heating the mixture to 100 ℃, decocting the mixture for 40-60 minutes, and filtering the decoction to form a first extracting solution and filter residues; adding water 7-9 times the weight of the filter residue into the filter residue, heating to 100 ℃, decocting for 50-120 minutes, and filtering the decoction to form a second extracting solution; mixing the first extractive solution and the second extractive solution, and mixing to obtain extractive solution; preferably, the pH of the extract is adjusted to 7.0 before the addition of the microbial agent.

8. The method for preparing cistanche tubulosa-Chinese herbal medicine enzyme according to claim 5, wherein in the step (3), the addition amount of the microbial agent is 0.5-10% of the weight of the extracting solution.

9. The method for preparing cistanche tubulosa enzyme as claimed in claim 5, wherein in step (3), before adding the microbial inoculum, the strain is stored in a freeze-drying tube or a glycerol tube, and before inoculation, the strain is activated and then inoculated.

10. The method for preparing cistanche tubulosa-Chinese herbal medicine enzyme according to claim 5, wherein in the step (3), the fermentation temperature is 25-45 ℃; the filtering is carried out by adopting a 325-mesh sieve; the low-temperature distillation temperature is 75 ℃, and the time is 4-7 h; the sterilization temperature is 75-95 ℃, and the sterilization time is 10-30 min.

Technical Field

The invention belongs to the technical field of preparation of Chinese herbal medicine enzymes, and relates to a cistanche Chinese herbal medicine enzyme and a preparation method thereof.

Background

In recent years, scholars at home and abroad carry out extensive research on chemical components and pharmacological effects of the cistanche deserticola, so that the effects of the cistanche deserticola in tonifying kidney and strengthening yang and refreshing male functions are further verified to be remarkable, and the cistanche deserticola is found to express good activity in the aspects of enhancing the immunity of the organism, improving the endurance of the organism, resisting oxidation, regulating the functions of a nervous system and an endocrine system and the like; through the evaluation and demonstration of the national Weijian Commission experts group, cistanche is listed in the homology of medicine and food catalogue and is implemented in 2020.

At present, the cistanche deserticola industry is in an initial stage, and the cistanche deserticola fermentation and related Chinese herbal medicine formula fermentation technology is not widely applied; because the chemical components contained in the traditional Chinese medicinal materials are very complex, the traditional Chinese medicinal materials not only contain various effective components, but also contain toxic components. Generally, a small amount of toxic components are inevitably left in a traditional Chinese medicine extracting solution or a traditional Chinese medicine concentrated solution, so that toxic and side effects are caused. Meanwhile, the traditional Chinese medicine extract has bitter taste or peculiar smell and is not accepted by most people.

In view of this, this patent is filed.

Disclosure of Invention

In order to solve the problems in the prior art, the invention provides a cistanche Chinese herbal medicine enzyme and a preparation method thereof, and the obtained cistanche Chinese herbal medicine enzyme is a beverage, and is a Chinese herbal medicine plant composite enzyme beverage with the effects of warming and recuperating kidney yang, enhancing immunity, improving male functions and the like.

One objective of the invention is to provide a cistanche Chinese herbal medicine enzyme;

the invention also aims to provide a preparation method of the cistanche Chinese herbal medicine ferment.

The technical scheme adopted by the invention is as follows:

according to the cistanche salsa Chinese herbal medicine enzyme disclosed by the embodiment of the invention, the cistanche salsa Chinese herbal medicine enzyme is prepared from the following raw materials: cistanche, eucommia male flower, pumpkin seed, rhizoma polygonati, lycium ruthenicum, maca powder, honeysuckle, dandelion, houttuynia, purslane, poria cocos, gordon euryale seed, phaseolus calcaratus, fructus alpiniae oxyphyllae, lophatherum gracile, coix seed, raspberry and microbial inoculum.

The cistanche Chinese herbal medicine enzyme provided by the embodiment of the invention is prepared from the following raw materials in parts by weight:

40-70 parts of cistanche, 10-20 parts of eucommia male flowers, 10-20 parts of pumpkin seeds, 20-30 parts of rhizoma polygonati, 10-20 parts of lycium ruthenicum, 30-50 parts of maca powder, 5-10 parts of honeysuckle, 5-10 parts of dandelion, 5-10 parts of houttuynia cordata, 5-10 parts of purslane, 5-10 parts of poria cocos, 5-10 parts of gordon euryale seeds, 5-10 parts of phaseolus calcaratus, 5-10 parts of sharpleaf galangal fruit, 5-10 parts of lophatherum gracile, 5-10 parts of coix seeds and 5-10 parts of raspberry.

The cistanche Chinese herbal medicine enzyme provided by the embodiment of the invention is prepared from the following raw materials in parts by weight:

55 parts of cistanche, 15 parts of eucommia male flower, 15 parts of pumpkin seed, 25 parts of rhizoma polygonati, 15 parts of lycium ruthenicum, 40 parts of maca powder, 8 parts of honeysuckle, 8 parts of dandelion, 8 parts of houttuynia cordata, 8 parts of purslane, 7.5 parts of poria cocos, 7.5 parts of gordon euryale seed, 7.5 parts of phaseolus calcaratus, 7 parts of fructus alpiniae oxyphyllae, 7 parts of lophatherum gracile, 7 parts of semen coicis and 7 parts of raspberry.

According to the cistanche Chinese herbal medicine ferment, the strain in the microbial agent is one or a combination of at least two of bifidobacterium, lactobacillus, streptococcus, lactococcus, propionibacterium, leuconostoc, kluyveromyces, pediococcus, staphylococcus or bacillus.

The method comprises the following steps:

(1) cleaning Cistanchis herba, Eucommiae cortex male flower, semen Cucurbitae, rhizoma Polygonati, fructus Lycii, maca powder, flos Lonicerae, herba Taraxaci, herba Houttuyniae, herba Portulacae, Poria, semen euryales, semen Phaseoli, fructus Alpinae Oxyphyllae, folium Bambusae, Coicis semen and Rubi fructus, respectively crushing, sieving, mixing to obtain mixture;

(2) putting the mixture obtained in the step (1) into an extraction tank, adding water, extracting twice, and combining filtrates to form an extracting solution;

(3) and (3) adding golden orange juice, fig juice, orange juice and lemon juice into the extracting solution obtained in the step (2), uniformly mixing, adding a microbial agent, fermenting for 50-75h to obtain a fermentation liquid, filtering, distilling the filtered fermentation liquid at a low temperature to remove alcohol and methanol, sterilizing and homogenizing to obtain the cistanche Chinese herbal medicine enzyme.

Wherein in the step (1), the sieving is 80-mesh sieving.

In the step (2), the extraction specifically comprises: adding water which is 10-20 times of the weight of the mixture into the mixture to immerse all the raw materials in the water, heating the mixture to 55-65 ℃, preserving the heat, soaking the mixture for 6 hours, heating the mixture to 100 ℃, decocting the mixture for 40-60 minutes, and filtering the decoction to form a first extracting solution and filter residues; adding water 7-9 times the weight of the filter residue into the filter residue, heating to 100 ℃, decocting for 50-120 minutes, and filtering the decoction to form a second extracting solution; mixing the first extractive solution and the second extractive solution, and mixing to obtain extractive solution; preferably, the pH of the extract is adjusted to 7.0 before the addition of the microbial agent.

Wherein in the step (3), the addition amount of the microbial inoculum is 0.5-10% of the weight of the extracting solution.

Wherein, in the step (3), before the microbial agent is added, the strain is placed in a freeze-drying tube or a glycerol tube for storage, and the strain is activated and then inoculated before inoculation.

Wherein in the step (3), the fermentation temperature is 25-45 ℃; the filtering is performed by adopting a 325-mesh sieve.

Wherein the low-temperature distillation temperature is 75 ℃, and the time is 4-7 h.

Wherein the sterilization temperature is 75-95 ℃, and the sterilization time is 10-30 min.

Lophatherum gracile is used as a medicine and has the effects of cooling, relieving fever, promoting urination and inducing spawning; the main treatment is as follows: chest fever, cough, hemoptysis, toxic heat wind, thirst, erysipelas, phlegm, mania, vexation, aphonia, headache, palpitation, epidemic, vexation, and parasite killing, and spleen and stomach relieving; can also be used as pasture.

The eucommia male flower has high medicinal value from the ancient time. The research proves that the natural active components of the eucommia male flower have the functions of soothing the nerves, tranquilizing and easing pain, and the sleep can be obviously improved after long-term use. In addition, the eucommia male flower contains collagen essential to human body, has the effect of promoting muscle development and strengthening, has obvious anti-fatigue effect of active ingredient lignans, and has obvious effect on people who lack exercise for a long time doing indoor work. Scientific research finds that: the eucommia male flower specially contains more than 60 effective plant components, such as lignins, iridoids, phenylpropanoid active substances (such as geniposide acid, chlorogenic acid and aucubin), pinoresinol diglucoside, flavones, alkaloids, amino acids, polysaccharides, mineral elements Zn, Mn, Cu, Fe, Ca, P, B, Mg and K, wherein the amino acid content reaches 21.47%, the calcium content reaches 0.92%, and the zinc content reaches 51Mg/100g, which are respectively 1.8 times and 5.2 times of eucommia leaves, the effective components are most comprehensive and higher in content, the eucommia male flower collects the essence of all eucommia, so that the eucommia male flower has all health care effects of the plant eucommia, and has particularly obvious effects on strengthening liver, tonifying kidney, relaxing bowels, improving sleep and reducing three highs.

Experiments prove that the effects of warming and invigorating kidney yang, enhancing immunity and improving male functions are obviously enhanced due to the fact that the eucommia male flowers benefit essence and qi, strengthen bones and muscles and interact with the lophatherum gracile in the invention to clear heart and remove dryness so that the health-care function of the eucommia male flowers is better exerted, and the cistanche Chinese herbal medicine enzyme disclosed by the invention has the effects of warming and invigorating kidney yang, enhancing immunity and improving male functions more obviously.

The fermentation process can convert macromolecular substances of effective components in the traditional Chinese medicine concentrated solution into micromolecular substances, and the micromolecular substances have higher pharmacological activity and are easier to be absorbed by human bodies, so that the utilization rate of the effective components by the human bodies can be improved, toxic substances in the traditional Chinese medicines can be decomposed in the fermentation process, the toxic and side effects of the traditional Chinese medicines are reduced, the obtained traditional Chinese medicine ferment has good taste, and the bitter taste or the peculiar smell is improved. The traditional Chinese medicine ferment is processed by adopting the modern process technology, overcomes the defects of low pharmacological activity, difficult absorption of active ingredients, slow response, toxic and side effects and poor taste of the existing ferment, retains the original active substances, improves the efficacy of the original plants, and achieves the effects of warming and recuperating kidney yang, enhancing immunity and improving male functions.

Compared with the prior art, the invention has the beneficial effects that:

(1) according to the cistanche Chinese herbal medicine enzyme disclosed by the invention, the traditional Chinese medicine concentrated solution is fermented, so that the safety and stability of the formula are improved, the toxic and side effects are reduced, the obtained traditional Chinese medicine enzyme has good taste, and the bitter taste or peculiar smell is improved;

(2) the cistanche Chinese herbal medicine enzyme provided by the invention has the effects of warming and recuperating kidney yang, enhancing immunity and improving male functions.

(3) The golden orange juice, the fig juice, the orange juice and the lemon juice are added according to a certain proportion, so that the mouthfeel is improved, and the acceptability of consumers is improved.

(4) The raw materials of the invention are all materials which are homologous in medicine and food, and the safety of the formula is improved.

(5) The efficacy of the Chinese herbal medicine formula is combined with the activity of the biological enzyme, so that the functionality of the product is improved.

(6) The alcohol distillation process is added, harmful substances such as methanol and the like are removed, and the safety of the product is guaranteed.

Detailed Description

In order to make the objects, technical solutions and advantages of the present invention more apparent, the technical solutions of the present invention will be described in detail below. It is to be understood that the described embodiments are merely exemplary of the invention, and not restrictive of the full scope of the invention. All other embodiments, which can be derived by a person skilled in the art from the examples given herein without any inventive step, are within the scope of the present invention.

In some more specific embodiments, the cistanche salsa herbal ferment is prepared from the following raw materials in parts by weight:

40-70 parts of cistanche, 10-20 parts of eucommia male flowers, 10-20 parts of pumpkin seeds, 20-30 parts of rhizoma polygonati, 10-20 parts of lycium ruthenicum, 30-50 parts of maca powder, 5-10 parts of honeysuckle, 5-10 parts of dandelion, 5-10 parts of houttuynia cordata, 5-10 parts of purslane, 5-10 parts of poria cocos, 5-10 parts of gordon euryale seeds, 5-10 parts of phaseolus calcaratus, 5-10 parts of sharpleaf galangal fruit, 5-10 parts of lophatherum gracile, 5-10 parts of coix seeds and 5-10 parts of raspberry;

the preparation method of the cistanche Chinese herbal medicine ferment comprises the following steps:

(1) cleaning Cistanchis herba, Eucommiae cortex male flower, semen Cucurbitae, rhizoma Polygonati, fructus Lycii, maca powder, flos Lonicerae, herba Taraxaci, herba Houttuyniae, herba Portulacae, Poria, semen euryales, semen Phaseoli, fructus Alpinae Oxyphyllae, folium Bambusae, Coicis semen and Rubi fructus, respectively crushing, sieving, mixing to obtain mixture;

(2) putting the mixture obtained in the step (1) into an extraction tank, adding water, extracting twice, and combining filtrates to form an extracting solution;

(3) and (3) adding golden orange juice, fig juice, orange juice and lemon juice into the extracting solution obtained in the step (2), uniformly mixing, adding a microbial agent, fermenting for 50-75h to obtain a fermentation liquid, filtering, distilling the filtered fermentation liquid at a low temperature to remove alcohol and methanol, sterilizing and homogenizing to obtain the cistanche Chinese herbal medicine enzyme.

Example 1

The embodiment provides a cistanche salsa Chinese herbal medicine enzyme prepared from the following raw materials:

40g of cistanche, 20g of eucommia male flower, 10g of pumpkin seed, 30g of rhizoma polygonati, 10g of lycium ruthenicum, 50g of maca powder, 5g of honeysuckle, 10g of dandelion, 5g of houttuynia cordata, 10g of purslane, 10g of poria cocos, 5g of gordon euryale seed, 10g of phaseolus calcaratus, 10g of fructus alpiniae oxyphyllae, 5g of lophatherum gracile, 5g of semen coicis and 5g of raspberry.

The strain in the microbial agent is Kluyveromyces and Pediococcus added at a ratio of 1: 2.

The preparation method of the cistanche Chinese herbal medicine enzyme comprises the following steps:

(1) cleaning Cistanchis herba, Eucommiae cortex male flower, semen Cucurbitae, rhizoma Polygonati, fructus Lycii, maca powder, flos Lonicerae, herba Taraxaci, herba Houttuyniae, herba Portulacae, Poria, semen euryales, semen Phaseoli, fructus Alpinae Oxyphyllae, folium Bambusae, Coicis semen and Rubi fructus, respectively crushing, sieving with 80 mesh sieve, and mixing to obtain mixture;

(2) placing the mixture obtained in the step (1) in an extraction tank, adding water which is 20 times of the weight of the mixture into the mixture to immerse all the raw materials in the water, heating the mixture to 55 ℃, preserving the heat, soaking the mixture for 6 hours, heating the mixture to 100 ℃, decocting the mixture for 60 minutes, and filtering the decoction to form a first extracting solution and filter residues; adding water 7 times the weight of the filter residue into the filter residue, heating to 100 ℃, decocting for 120 minutes, and filtering the decoction to form a second extracting solution; mixing the first extractive solution and the second extractive solution, and mixing to obtain extractive solution;

(3) adjusting the pH value of the extracting solution obtained in the step (2) to 7.0, adding 0.5 wt% of kumquat juice, 0.1 wt% of fig juice, 1 wt% of orange juice and 0.1 wt% of lemon juice into the extracting solution, uniformly mixing, then adding 10 wt% of microbial inoculum based on the weight of the extracting solution, fermenting for 75 hours at 25 ℃, filtering the obtained fermentation liquor by adopting a 325-mesh sieve, distilling the filtered fermentation liquor at 75 ℃ for 47 hours at low temperature, removing alcohol and methanol, sterilizing for 30 minutes at 75 ℃, homogenizing to obtain the cistanche Chinese herbal medicine enzyme, placing the strain into a freeze-drying tube or a glycerol tube for preservation before adding the microbial inoculum, respectively activating the strain before inoculating, then inoculating, and when inoculating, ensuring that the total viable bacteria amount of the microbial inoculum is 5.0 multiplied by 10⁸cfu/g。

Example 2

The embodiment provides a cistanche salsa Chinese herbal medicine enzyme prepared from the following raw materials:

70g of cistanche, 10g of eucommia male flower, 20g of pumpkin seed, 20g of rhizoma polygonati, 10g of lycium ruthenicum, 30g of maca powder, 10g of honeysuckle, 5g of dandelion, 10g of houttuynia cordata, 5g of purslane, 5g of poria cocos, 10g of gordon euryale seed, 5g of phaseolus calcaratus, 5g of fructus alpiniae oxyphyllae, 10g of lophatherum gracile, 10g of semen coicis and 10g of raspberry.

The strain in the microbial agent is added with Lactobacillus and Staphylococcus in a ratio of 3: 2;

the preparation method of the cistanche Chinese herbal medicine enzyme comprises the following steps:

(1) cleaning Cistanchis herba, Eucommiae cortex male flower, semen Cucurbitae, rhizoma Polygonati, fructus Lycii, maca powder, flos Lonicerae, herba Taraxaci, herba Houttuyniae, herba Portulacae, Poria, semen euryales, semen Phaseoli, fructus Alpinae Oxyphyllae, folium Bambusae, Coicis semen and Rubi fructus, respectively crushing, sieving with 80 mesh sieve, and mixing to obtain mixture;

(2) placing the mixture obtained in the step (1) in an extraction tank, adding water which is 20 times of the weight of the mixture into the mixture to immerse all the raw materials in the water, heating the mixture to 55 ℃, preserving the heat, soaking the mixture for 6 hours, heating the mixture to 100 ℃, decocting the mixture for 60 minutes, and filtering the decoction to form a first extracting solution and filter residues; adding water 7 times the weight of the filter residue into the filter residue, heating to 100 ℃, decocting for 50-120 minutes, and filtering the decoction to form a second extracting solution; mixing the first extractive solution and the second extractive solution, and mixing to obtain extractive solution;

(3) adjusting the pH value of the extracting solution obtained in the step (2) to 7.0, adding 0.5 wt% of kumquat juice, 0.1 wt% of fig juice, 1 wt% of orange juice and 0.1 wt% of lemon juice into the extracting solution, uniformly mixing, then adding 1 wt% of microbial inoculum of the weight of the extracting solution, fermenting for 50 hours at 45 ℃, filtering the obtained fermentation liquor by adopting a 325-mesh sieve, distilling the filtered fermentation liquor at 75 ℃ for 7 hours at low temperature, removing alcohol and methanol, sterilizing for 10 minutes at 95 ℃, homogenizing to obtain the cistanche Chinese herbal medicine enzyme, placing the strain into a freeze-drying tube or a glycerol tube for preservation before adding the microbial inoculum, respectively activating the strain before inoculation, then inoculating, wherein the total viable bacteria amount of the microbial inoculum is 10.0 multiplied by 10 when inoculating, and the total viable bacteria amount of the microbial inoculum is 10.0 multiplied by 10⁸cfu/g。

Example 3

The embodiment provides a cistanche salsa Chinese herbal medicine enzyme prepared from the following raw materials:

55g of cistanche, 15g of eucommia male flower, 15g of pumpkin seed, 25g of rhizoma polygonati, 15g of lycium ruthenicum, 40g of maca powder, 8g of honeysuckle, 8g of dandelion, 8g of houttuynia cordata, 8g of purslane, 7.5g of poria cocos, 7.5g of gordon euryale seed, 7.5g of phaseolus calcaratus, 7g of fructus alpiniae oxyphyllae, 7g of lophatherum gracile, 7g of semen coicis and 7g of raspberry.

The strain in the microbial agent is Bifidobacterium and Leuconostoc, and is added at a ratio of 3: 1;

the preparation method of the cistanche Chinese herbal medicine enzyme comprises the following steps:

(1) cleaning Cistanchis herba, Eucommiae cortex male flower, semen Cucurbitae, rhizoma Polygonati, fructus Lycii, maca powder, flos Lonicerae, herba Taraxaci, herba Houttuyniae, herba Portulacae, Poria, semen euryales, semen Phaseoli, fructus Alpinae Oxyphyllae, folium Bambusae, Coicis semen and Rubi fructus, respectively crushing, sieving with 80 mesh sieve, and mixing to obtain mixture;

(2) placing the mixture obtained in the step (1) in an extraction tank, adding water 15 times of the weight of the mixture into the mixture to immerse all the raw materials in the water, heating the mixture to 60 ℃, preserving heat, soaking the mixture for 6 hours, heating the mixture to 100 ℃, decocting the mixture for 50 minutes, and filtering the decoction to form a first extracting solution and filter residue; adding water 8 times the weight of the filter residue into the filter residue, heating to 100 ℃, decocting for 100 minutes, and filtering the decoction to form a second extracting solution; mixing the first extractive solution and the second extractive solution, and mixing to obtain extractive solution;

(3) adjusting the pH value of the extracting solution obtained in the step (2) to 7.0, adding 0.5 wt% of kumquat juice, 0.1 wt% of fig juice, 1 wt% of orange juice and 0.1 wt% of lemon juice into the extracting solution, uniformly mixing, then adding 8 wt% of microbial inoculum based on the weight of the extracting solution, fermenting for 60 hours at 35 ℃, filtering the obtained fermentation liquor by adopting a 325-mesh sieve, distilling the filtered fermentation liquor at 75 ℃ for 4-7 hours at low temperature, removing alcohol and methanol, sterilizing for 10-30 minutes at 75-95 ℃, homogenizing to obtain the cistanche Chinese herbal medicine enzyme, placing the strain into a freeze-drying pipe or a glycerol pipe for preservation before adding the microbial inoculum, respectively activating the strain before inoculating, then inoculating, wherein during inoculating, the total viable count of the microbial inoculum is 3.0 x 10⁸cfu/g。

Example 4

The only difference between this example and example 3 is that the microbial agents used were Kluyveromyces and Pediococcus added at a viable bacteria ratio of 3: 1.

Example 5

The only difference between this example and example 3 is that the microbial agents used were Propionibacterium and Leuconostoc, and were added at a viable bacteria ratio of 3: 1.

Determination of raw materials

Comparative example 1

The only difference between this comparative example and example 3 is that the raw material was not supplemented with eucommia male flowers.

Comparative example 2

The only difference between this comparative example and example 3 is that the raw materials were not added with lophatherum gracile.

Comparative example 3

The only difference between this comparative example and example 3 is that no houttuynia cordata was added to the raw materials.

The results of comparing the cistanche Chinese herbal medicine enzymes obtained in the embodiments 3-5 and the comparative examples 1-3 and monitoring sexual life frequency, impotence, premature ejaculation, spermatorrhea, ejaculation disorder, fatigue and the like show that the eucommia male flowers, the lophatherum gracile and the houttuynia cordata added in the embodiments 3, 4 and 5 of the invention have the effects of warming and invigorating kidney yang, enhancing immunity and improving male functions and can be used for searching the reasons. Example 4 was slightly more effective in enhancing immunity and improving male sexual function than examples 3 and 5, probably because the effect of the ingredients was enhanced by Kluyveromyces and Pediococcus in a viable bacteria ratio of 3: 1.

Determination of amount of cistanche

Preparing the cistanche Chinese herbal medicine enzyme according to the raw materials and the preparation method of the embodiment 3, wherein the addition amounts of the cistanche are respectively adjusted to be 30g, 40g, 55g, 70g and 80 g; obtaining the optimal addition range and the optimal addition amount of the cistanche according to sensory evaluation criteria.

Determination test of addition amounts of kumquat juice, fig juice, orange juice and lemon juice

1. Determination of the amount of juice of golden orange

Preparing the cistanche Chinese herbal medicine enzyme according to the raw materials and the preparation method in the embodiment 3, wherein only the addition amounts of the golden orange juice are respectively adjusted to be 0.1 wt%, 0.3 wt%, 0.5 wt%, 0.7 wt% and 0.9 wt%; obtaining the optimal addition amount of golden orange juice, fig juice, orange juice and lemon juice according to sensory evaluation standard.

2. Determination of amount of Ficus Simplicissima fruit juice

Preparing the cistanche Chinese herbal medicine enzyme according to the raw materials and the preparation method of the embodiment 3, wherein the addition amounts of the fig juice are respectively adjusted to be 0.06 wt%, 0.08 wt%, 0.1 wt%, 0.12 wt% and 0.14 wt%; obtaining the optimal addition amount of the fig juice according to sensory evaluation criteria.

3. Determination of the amount of orange juice added

Preparing the cistanche Chinese herbal medicine enzyme according to the raw materials and the preparation method in the embodiment 3, wherein only the addition amounts of the orange juice are respectively adjusted to be 0.6 wt%, 0.8 wt%, 1 wt%, 1.2 wt% and 1.4 wt%; and obtaining the optimal addition amount of the orange juice according to sensory evaluation criteria.

4. Determination of the amount of lemon juice added

Preparing the cistanche Chinese herbal medicine enzyme according to the raw materials and the preparation method of the embodiment 3, wherein only the addition amounts of the lemon juice are respectively adjusted to be 0.06 wt%, 0.08 wt%, 0.1 wt%, 0.12 wt% and 0.14 wt%; obtaining the optimum addition amount of lemon juice according to sensory evaluation standard.

The method for evaluating the sensory quality of the beverage comprises the following steps:

in the whole sensory evaluation process, 24 experienced evaluators perform sensory evaluation on the cistanche Chinese herbal medicine enzyme products in 4 aspects of color, smell, taste and tissue form, the full score is 100, and the higher the score is, the better the state and the better the quality of the beverage product are, and the better the beverage product is. Referring to the national standard GB 7101-.

TABLE 1 sensory quality evaluation criteria

The result shows that according to sensory evaluation, the optimal value of the addition amount of the cistanche salsa is 55g, and the optimal range is 40-70 g; the preferred addition amount of kumquat juice, fig juice, orange juice and lemon juice is 0.5 wt%, fig juice 0.1 wt%, orange juice 1 wt% and lemon juice 0.1 wt%.

Evaluation of human body efficacy by improving male functional beverage

51 males of 35-60 years old are selected to perform functional test on the product, the male males take 30 ml once a day, the product efficacy is evaluated for one week in a period, 23 relevant indexes of the items are drawn up to perform questionnaire survey, and finally, preliminary statistical results of the product efficacy are obtained as shown in tables 2, 3 and 4.

TABLE 2 basic status of the Male investigational population

TABLE 3 Pre-drug conditions Male dysfunctional conditions

TABLE 4 improvement of male sexual dysfunction after drug administration

Through tracking and visiting, the cistanche salsa Chinese herbal medicine enzyme disclosed by the invention is found to have a good user response effect and has the effect of improving sexual life.

And (3) fatigue resistance test:

70 SPF-grade healthy adult ICR mice are divided into 7 groups, and the test enzyme beverages prepared in examples 3-5 and comparative examples 1-3 and pure water (control group) are respectively administered to the mice, and the mice are subjected to intragastric administration of 0.2mL each day for 3 weeks, and then the weight-bearing swimming time detection of the mice is carried out according to the test method of the physical fatigue relieving function test in the health food test and evaluation technical Specification 2003 edition.

The results of the weight swimming test of each group of mice are shown in Table 5.

TABLE 5 mouse weight bearing swimming test results

The inventor also makes a fatigue model by binding the mice, and administers the ferment beverage of the invention in the example 3 for 3 weeks, wherein the middle dose group is subjected to intragastric administration of 0.2mL every day, the low dose group is subjected to intragastric administration of 0.1mL every day, and the high dose group is subjected to intragastric administration of 1mL every day. Testis weight was slightly decreased in the medium dose group, and reached the highest level in both the high dose and low dose groups, while testis index was the lowest in the medium dose group. The sperm density reaches the highest dose in clinic, and the forward movement sperm ratio also has a high level, which indicates that the enzyme drink with the dose in clinic has a certain improvement effect on the sperm index of the fatigue animal model caused by binding. In the clinical dose-treated group, the blood levels of inhibin B and testosterone were increased in mice compared to the model group, while the GnRH level was decreased. For testis and epididymis tissues, the ferment beverage provided by the invention shows a certain trend for improving male reproductive indexes of a mouse fatigue model.

By detecting the testosterone level in blood, adenine induces the kidney deficiency of mice and is accompanied with the descending trend of the testosterone level, while the ferment beverage provided by the invention has a certain trend of increasing the testosterone level.

The above description is only for the specific embodiments of the present invention, but the scope of the present invention is not limited thereto, and any person skilled in the art can easily conceive of the changes or substitutions within the technical scope of the present invention, and all the changes or substitutions should be covered within the scope of the present invention. Therefore, the protection scope of the present invention shall be subject to the protection scope of the appended claims.