阅读说明：本技术 一种植物外源蛋白提取纯化设备 (Plant foreign protein extraction and purification equipment ) 是由 赵志刚 于 2021-05-17 设计创作，主要内容包括：一种植物外源蛋白提取纯化设备,涉及蛋白提取纯化技术领域；包括下箱体,下箱体上端设有一体成型的上箱体,上箱体上端设有开口,开口处设有可开合的盖体,上箱体内设有可转动的粉碎罐,盖体下端设有延伸至粉碎罐内部并能够旋转清理粉碎罐的旋转粉碎组件,盖体上端设有驱动旋转粉碎组件的驱动电机,下箱体内部上端安装有蝶式离心机,下箱体内部下端安装有超滤管。现有技术中蛋白纯化方法周期长、成本高、方法不通用,且组织细胞初步处理形成浆状物不易清理的问题,这种转基因植物外源蛋白提取纯化方法,一是周期短,两个月即可,大大提高工作效率；二是成本低,一万多的成本即可；三是通用性,该方法适用于动植物中各种目的蛋白的分离纯化。(A plant foreign protein extraction and purification device relates to the technical field of protein extraction and purification; including lower box, lower box upper end is equipped with integrated into one piece's last box, goes up the box upper end and is equipped with the opening, and the opening part is equipped with the lid that can open and shut, goes up and is equipped with rotatable crushing jar in the box, and the lid lower extreme is equipped with the rotatory crushing unit who extends to crushing jar inside and can rotate the clearance crushing jar, and the lid upper end is equipped with the driving motor of the rotatory crushing unit of drive, and butterfly centrifuge is installed to the inside upper end of lower box, and the ultrafiltration pipe is installed to the inside lower extreme of lower box. The method for extracting and purifying the exogenous protein of the transgenic plant has the advantages that the period is short, the period is two months, and the working efficiency is greatly improved; secondly, the cost is low, namely more than ten thousand of costs are needed; thirdly, the method is universal, and is suitable for separating and purifying various target proteins in animals and plants.)

1. A plant exogenous protein extraction and purification device comprises a lower box body (1), an upper box body (2), a crushing tank (3), a rotary crushing component (4), a butterfly centrifuge (5) and an ultrafiltration pipe (6), and is characterized in that the upper end of the lower box body (1) is provided with the integrally formed upper box body (2), the upper end of the upper box body (2) is provided with an opening, an opening is provided with a cover body (8) which can be opened and closed, the upper box body (2) is internally provided with the rotatable crushing tank (3), the lower end of the cover body (8) is provided with the rotary crushing component (4) which extends into the crushing tank (3) and can rotationally clean the crushing tank (3), the rotary crushing component (4) and the crushing tank (3) reversely rotate, the upper end of the cover body (8) is provided with a driving motor for driving the rotary crushing component (4), the butterfly centrifuge (5) is installed at the upper end inside the lower box body (1), ultrafiltration pipe (6) are installed to box (1) inside lower extreme down, row's sediment pipe (51) and light liquid pipe (52) are installed to butterfly centrifuge (5) upper end, light liquid pipe (52) and ultrafiltration pipe (6) one end intercommunication setting of butterfly centrifuge (5), smash the feed inlet intercommunication of jar (3) lower extreme and butterfly centrifuge (5) upper end, and smash jar (3) and the junction of butterfly centrifuge (5) and be equipped with electric valve.

2. The plant foreign protein extraction and purification device according to claim 1, wherein a feeding pipe is arranged at the lower end of the crushing tank (3), the crushing tank (3) is rotatably arranged at the bottom in the upper box body (2) through the feeding pipe, a cam (9) is fixed on the outer side of the feeding pipe, an inflator (10) which is rotated by the cam (9) to perform circulating inflation is arranged at the upper end of the lower box body (1), and an air inlet pipe (11) is arranged between an air outlet of the inflator (10) and the cover body (8).

3. The plant foreign protein extraction and purification device according to claim 1, wherein the lower end of the crushing tank (3) is hemispherical, the upper end of the crushing tank (3) is cylindrical, the rotary crushing assembly (4) comprises a fixing ring (41), an arc-shaped rod (42), a scraper (43) and a stirring shaft (44), the arc-shaped rod (42) is fixed at the lower end of the fixing ring (41) in a crossed manner, the vertical scrapers (43) are arranged around the upper end of the fixing ring (41), the stirring shaft (44) is fixed at the upper end of the crossed position of the arc-shaped rod (42), and the upper end of the stirring shaft (44) is fixedly connected with an output shaft of a driving motor at the top of the cover body (8).

4. The apparatus for extracting and purifying a plant foreign protein according to claim 3, wherein a wave-shaped pounding piece (46) is fixedly connected to the outer side of the arc-shaped rod (42).

5. The device for extracting and purifying the plant foreign protein according to claim 3, wherein the scraping plate (43) is V-shaped, one side of the pointed cone of the scraping plate (43) is connected with the wall surface of the crushing tank (3), a plurality of groups of stirring wheels (45) are connected to the inner side of the scraping plate (43), and the central shaft of each stirring wheel (45) extends into the scraping plate (43) and is fixedly provided with a driven gear (452).

6. The device for extracting and purifying the foreign vegetable protein according to claim 5, wherein the shearing pieces (451) are fixedly connected to the outer periphery of the stirring wheel (45), a rack (12) for driving a driven gear (452) is arranged in the scraping plate (43), a wave-shaped chute (31) is arranged at the upper end of the inside of the crushing tank (3), and the upper end of the rack (12) is slidably arranged with the chute (31).

7. The plant foreign protein extraction and purification device according to claim 1, wherein the backflow pipe assembly (7) comprises a dividing pipe (71), a liquid pump (72) and a backflow pipe (73), one end of the dividing pipe (71) is fixedly communicated with the light liquid pipe (52), the other end of the dividing pipe (71) is provided with the liquid pump (72), the backflow pipe (73) is arranged between the liquid pump (72) and the cover body (8), and the light liquid pipe (52) is provided with a valve (521) at the lower end of the dividing pipe (71).

8. The apparatus for extracting and purifying a plant foreign protein according to claim 1, wherein the slag discharge pipe (51) extends to the outside of the lower tank (1).

9. The device for extracting and purifying the foreign vegetable protein as claimed in claim 2, wherein the upper end of the cover body (8) is provided with an integrally formed external thread pipe (81), the upper end of the air inlet pipe (11) is provided with an integrally formed internal thread pipe (111), and the internal thread pipe (111) is in matching threaded connection with the external thread pipe (81).

Technical Field

The invention relates to the technical field of protein extraction and purification, in particular to a plant exogenous protein extraction and purification device.

Background

The transgenic plant transfers specific exogenous target gene into receptor organism, and the exogenous protein expressed by the exogenous gene plays a biological role. The safety of the exogenous protein needs to be proved by experiments, the development of safety experiments firstly needs to extract and purify the exogenous protein from plants, and experimental research is carried out on the protein. The separation and purification of proteins is a difficult and burdensome task, and it is necessary to select an appropriate purification method to increase the purity and quantity of the obtained proteins according to the characteristics of the proteins.

The principle of the existing protein purification is as follows: the different proteins have different amino acid sequences and spatial structures, so that the proteins have differences in physical, chemical, biological and other properties, and a set of reasonable protein purification schemes can be designed by utilizing the differences in the properties of the proteins to be separated and other proteins;

the existing protein purification method comprises the following steps: the purification of proteins is roughly divided into two stages, a coarse separation stage and a fine purification stage. The crude separation stage mainly separates the target protein from other cellular components such as RNA, DNA, etc., and the common method is ammonium sulfate precipitation. The purpose of the fine purification stage is to distinguish the target protein from other proteins with similar size and physicochemical properties, and the following methods are commonly used: gel filtration chromatography, ion exchange chromatography, hydrophobic chromatography, affinity chromatography, etc.;

the prior protein purification method has the following defects: the primary treatment of tissue cells to form a slurry is not easy to clean.

Disclosure of Invention

The invention aims to solve the technical problems that the protein purification method in the prior art is long in period, high in cost and not universal, and the pulp formed by primary treatment of histiocytes is not easy to clean.

The purpose and the effect of the invention are achieved by the following specific technical means:

a plant exogenous protein extraction and purification device comprises a lower box body, an upper box body, a crushing tank, a rotary crushing assembly, a butterfly centrifuge and an ultrafiltration tube, wherein the upper end of the lower box body is provided with an integrally formed upper box body, the upper end of the upper box body is provided with an opening, the opening is provided with a cover body which can be opened and closed, the upper box body is internally provided with a rotatable crushing tank, the lower end of the cover body is provided with the rotary crushing assembly which extends into the crushing tank and can rotationally clean the crushing tank, and the rotary crushing assembly and the crushing tank rotate oppositely, a driving motor for driving the rotary crushing assembly is arranged at the upper end of the cover body, a butterfly centrifuge is arranged at the upper end inside the lower box body, an ultrafiltration tube is arranged at the lower end inside the lower box body, a residue discharge tube and a light liquid tube are arranged at the upper end of the butterfly centrifuge, the light liquid tube of the butterfly centrifuge is communicated with one end of the ultrafiltration tube, the lower end of the crushing tank is communicated with a feed inlet at the upper end of the butterfly centrifuge, and an electric valve is arranged at the joint of the crushing tank and the butterfly centrifuge.

Preferably, smash a jar lower extreme and be equipped with the unloading pipe, smash a jar and rotate through the unloading pipe and set up the bottom in last box, and unloading outside of tubes side is fixed with the cam, and the inflator that is inflated circularly by the cam rotation is installed to lower box upper end, installs the intake pipe between the gas outlet of inflator and the lid.

Preferably, smash a jar lower extreme and be hemispherical, smash a jar upper end and be cylindricly, rotatory crushing unit is including solid fixed ring, arc pole, scraper blade and (mixing) shaft, and solid fixed ring lower extreme cross fixation has the arc pole, gu all be equipped with vertical scraper blade around the fixed ring upper end, arc pole cross department upper end is fixed with the (mixing) shaft, the (mixing) shaft upper end and lid top driving motor's output shaft fixed connection.

Preferably, the outer side of the arc-shaped rod is fixedly connected with the smashing piece in a wave shape.

Preferably, the scraper is V-shaped, one side of the pointed cone of the scraper is connected with the wall surface of the crushing tank, a plurality of groups of stirring wheels are connected in a switching way on the inner side of the scraper, and a central shaft of each stirring wheel extends into the scraper and is fixedly provided with a driven gear.

Preferably, shear blades are fixedly connected to the periphery of the outer side of the stirring wheel, a rack for driving a driven gear is arranged in each scraper, a wavy sliding groove is formed in the upper end of the inner portion of the crushing tank, and the upper end of each rack is arranged in a sliding manner with the corresponding sliding groove.

Preferably, the backflow pipe assembly comprises a flow dividing pipe, a liquid pump and a backflow pipe, one end of the flow dividing pipe is fixedly communicated with the light liquid pipe, the liquid pump is installed at the other end of the flow dividing pipe, the backflow pipe is arranged between the liquid pump and the cover body, and the light liquid pipe is provided with a valve at the lower end of the flow dividing pipe.

Preferably, the slag discharge pipe extends to the outer side of the lower box body.

Preferably, the upper end of the cover body is provided with an integrally formed external threaded pipe, the upper end of the air inlet pipe is provided with an integrally formed internal threaded pipe, and the internal threaded pipe is in threaded connection with the external threaded pipe in a matched mode.

Has the advantages that:

1. the plant foreign protein extraction and purification equipment is provided with a rotary crushing assembly, after a driving motor is started, the rotary crushing assembly rotates, an arc rod stirs and crushes tissue cells, meanwhile, a crushing piece on the outer side of the arc rod rotationally rolls the tissue cells, the tissue cells are crushed into pulp, a scraper scrapes off pulp adhered to the inner wall of a stirring tank, the scraper drives the stirring wheel to shear the pulp when rotating, a rack rotates along with the scraper and is acted by a waveform sliding groove, the rack moves up and down, a driven gear is driven to rotate in a reciprocating manner, the stirring wheel drives the shearing piece to rotate to stir the pulp, and the pulp is uniformly crushed; the counter-rotation of the rotating milling assembly and the milling pot also allows tissue cells to be processed more quickly for initial processing.

2. This kind of plant foreign protein draws purification equipment is provided with the back flow subassembly, and after the liquid pump was started, protein and moisture got into the shunt tubes, and the waste residue of production was discharged through the scum pipe, then got into crushing jar through the back flow, and moisture and scraper blade cooperation scraped the thick liquid thing of the interior adhesion of crushing jar for crushing jar can further be cleared up cleanly, also can not cause the wasting of resources, and circulation 3-5 separation improves the utilization ratio of protein.

3. This kind of plant exogenous protein draws purification equipment is provided with the inflater, and the unloading pipe rotates and drives the cam and rotate, and the piston rod of inflater is moved in the cam top, and the gas of production then gets into in smashing the jar through the intake pipe for smash the interior pressure increase of jar, make the tissue receive the easier breakage of pressure influence, improve the efficiency of processing.

Description of the drawings:

FIG. 1 is a schematic sectional view of the plant foreign protein extraction and purification apparatus according to the present invention.

Figure 2 is a schematic view of a rotary size reduction assembly of the present invention.

FIG. 3 is a schematic top view of the squeegee of the present invention.

Fig. 4 is an enlarged schematic view of the invention at a in fig. 1.

Fig. 5 is a partial schematic view of the chute of the present invention.

In FIGS. 1-5: the device comprises a lower box body 1, a mounting plate 101, an upper box body 2, a crushing tank 3, a chute 31, a rotary crushing assembly 4, a fixing ring 41, an arc-shaped rod 42, a scraping plate 43, a stirring shaft 44, a stirring wheel 45, a shearing piece 451, a driven gear 452, a mashing piece 46, a butterfly centrifuge 5, a slag discharge pipe 51, a light liquid pipe 52, a valve 521, an ultrafiltration pipe 6, a backflow pipe assembly 7, a shunt pipe 71, a liquid suction pump 72, a backflow pipe 73, a cover body 8, an external threaded pipe 81, a cam 9, an inflator 10, an air inlet pipe 11, an internal threaded pipe 111 and a rack 12.

Detailed Description

The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments.

Referring to fig. 1-5, a plant exogenous protein extraction and purification device comprises a lower box body 1, an upper box body 2, a crushing tank 3, a rotary crushing assembly 4, a butterfly centrifuge 5 and an ultrafiltration tube 6, wherein the upper end of the lower box body 1 is provided with the integrally formed upper box body 2, the upper end of the upper box body 2 is provided with an opening, the opening is covered with a cover body 8, the lower end of the crushing tank 3 is provided with a blanking tube, the crushing tank 3 is rotatably connected to the bottom in the upper box body 2 through the blanking tube, the outer side of the upper box body 2 is provided with a motor, an output shaft of the motor is connected with the blanking tube through a synchronous wheel and a synchronous belt, the motor drives the crushing tank 3 and the rotary crushing assembly 4 to reversely rotate, the lower end of the cover body 8 is rotatably connected with the rotary crushing assembly 4 which extends into the crushing tank 3 and can rotatably clean the crushing tank 3, the upper end of the cover body 8 is provided with a driving motor, the driving motor drives the rotary crushing assembly 4 to rotate, crush and stir, the inner part of the lower box body 1 is fixedly connected with a mounting plate 101, the upper end of the mounting plate 101 is provided with a butterfly centrifuge 5, the lower end of the inner part of the lower box body 1 is provided with an ultrafiltration pipe 6, the upper end of the butterfly centrifuge 5 is provided with a slag discharge pipe 51 and a light liquid pipe 52, the light liquid pipe 52 of the butterfly centrifuge 5 is communicated with one end of the ultrafiltration pipe 6, a feed pipe at the lower end of the crushing tank 3 is communicated with a feed inlet at the upper end of the butterfly centrifuge 5, and the feed pipe is provided with an electric valve; open lid 8 and put into crushing jar 3 with histocyte, close box 2 with lid 8 lid, start driving motor and drive rotatory crushing unit 4 and rotate, smash the histocyte and be the pulpiness through rotatory crushing unit 4.

Wherein: a cam 9 is fixed on the outer side of the blanking pipe, an inflator 10 is installed at the upper end of the lower box body 1, a piston rod of the inflator 10 extends into the upper box body 2 and is connected with the outer side of the cam 9, a return spring is sleeved in the piston rod positioned in the upper box body 2, and an air inlet pipe 11 is installed between an air outlet of the inflator 10 and the cover body 8; after the motor drives crushing jar 3 and rotates, unloading pipe rotates and drives cam 9 and rotate, and cam 9 pushes up the piston rod of inflater 10, and the gas of production then gets into crushing jar 3 through intake pipe 11 in for the pressure increase in crushing jar 3 makes the tissue receive the easier breakage of pressure influence, improves the efficiency of processing, at 8 upper end installation relief valves of lid, pressure is too big then the relief valve is opened.

Wherein: the lower end of the crushing tank 3 is hemispherical, the upper end of the crushing tank 3 is cylindrical, the rotary crushing assembly 4 comprises a fixing ring 41, an arc rod 42, a scraping plate 43 and a stirring shaft 44, the arc rod 42 is fixed at the lower end of the fixing ring 41 in a crossed manner, vertical scraping plates 43 are arranged around the upper end of the fixing ring 41, the stirring shaft 44 is fixed at the upper end of the crossed position of the arc rod 42, the upper end of the stirring shaft 44 is fixedly connected with an output shaft of a driving motor at the top of the cover body 8, a wave-shaped crushing sheet 46 is fixedly connected to the outer side of the arc rod 42, the scraping plate 43 is V-shaped, one side of a pointed cone of the scraping plate 43 is connected with the wall surface of the crushing tank 3, a plurality of groups of stirring wheels 45 are switched on the inner side of the scraping plate 43, a driven gear 452 is fixedly installed in the stirring wheel 45, shearing sheets 451 are fixedly connected around the outer side of the stirring wheel 45, a rack 12 for driving the driven gear 452 is arranged in the scraping plate 43, a wave-shaped chute 31 is arranged at the upper end in the crushing tank 3, the upper end of the rack 12 is arranged in a sliding way with the sliding chute 31; after the driving motor is started, the rotary crushing assembly 4 rotates, the arc-shaped rod 42 stirs and crushes the histiocytes, meanwhile, the crushing piece 46 outside the arc-shaped rod 42 rotationally rolls the histiocytes to crush the histiocytes into pulp, the scraper 43 scrapes off the pulp adhered to the inner wall of the stirring tank 3, the stirring wheel 45 is driven to shear the pulp when the scraper 43 rotates, the rack 12 rotates along with the scraper 43 and is acted by the waveform chute 31 to move up and down to drive the driven gear 452 to rotate in a reciprocating way, and the stirring wheel 45 drives the shearing piece 451 to rotate to stir the pulp, so that the pulp is uniformly crushed; the counter-rotation of the rotating milling assembly 4 and the milling pot 3 also allows the tissue cells to be processed more quickly for initial processing.

Wherein: the backflow pipe assembly 7 comprises a dividing pipe 71, a liquid pump 72 and a backflow pipe 73, one end of the dividing pipe 71 is fixedly communicated with the light liquid pipe 52, the liquid pump 72 is installed at the other end of the dividing pipe 71, the backflow pipe 73 is installed between the liquid pump 72 and the cover body 8, a valve 521 is arranged at the lower end of the light liquid pipe 52, and the slag discharge pipe 51 extends to the outer side of the lower box body 1; the thick liquid thing gets into butterfly centrifuge 5 through the unloading pipe and carries out solid-liquid separation, the protein and the moisture of initial gross separation get into light liquid pipe 52, after starting drawing liquid pump 72, protein and moisture get into shunt tubes 71, the waste residue of production is discharged through row's cinder pipe 51, then get into crushing jar 3 through back flow 73, moisture scrapes the thick liquid thing of attaching the adhesion in crushing jar 3 with the cooperation of scraper blade 43, make crushing jar 3 further clear up, can not cause the wasting of resources yet, 3-5 separation circulate, the utilization ratio of protein is improved.

Wherein: the upper end of the cover body 8 is provided with an integrally formed external threaded pipe 81, the upper end of the air inlet pipe 11 is provided with an integrally formed internal threaded pipe 111, and the internal threaded pipe 111 is in matched threaded connection with the external threaded pipe 81; through threaded connection cooperation installation, it is more convenient to install and dismantle.

The working principle is as follows: firstly, the cover body 8 is opened to put the histiocyte into the crushing tank 3, the cover body 8 is covered on the box body 2, the air inlet pipe 11 is in threaded connection with the external threaded pipe 81 at the upper end of the cover body 8 through the internal threaded pipe 111, the driving motor is started to drive the rotary crushing assembly 4 to rotate, simultaneously the starting motor drives the crushing tank 3 and the rotary crushing assembly 4 to reversely rotate, the arc-shaped rod 42 stirs and crushes the histiocyte, simultaneously the crushing piece 46 at the outer side of the arc-shaped rod 42 carries out rotary grinding on the histiocyte to crush the histiocyte into pulp, the scraper 43 scrapes off the pulp adhered to the inner wall of the stirring tank 3, the stirring wheel 45 is driven to shear the pulp when the scraper 43 rotates, the rack 12 follows the scraper 43 to rotate and is acted by the wave-shaped chute 31 to move up and down to drive the driven gear 452 to rotate back and forth, the stirring wheel 45 drives the shearing piece 451 to rotate to stir the pulp and crush the pulp evenly, opening an electric valve of a feeding pipe, starting the butterfly centrifuge 5, enabling the pulp to enter the butterfly centrifuge 5, starting the butterfly centrifuge 5 to perform solid-liquid separation on the pulp, enabling protein and moisture to flow out of a light liquid pipe 52, discharging solid residues from a residue discharge pipe 51, starting a liquid pump 72, pumping a mixture of the moisture and the protein into a diversion pipe 71 from the light liquid pipe 52, enabling the mixture to enter a backflow pipe 73 through the liquid pump 72, enabling the mixture to enter the crushing tank 3, continuously stirring the inside of the crushing tank 3 through a rotary crushing assembly 4, enabling the liquid to continuously enter the butterfly centrifuge 5 through the feeding pipe, performing solid-liquid separation again, circulating for 3-5 times, closing the liquid pump 72, opening a valve 521, and enabling the protein to enter an ultrafiltration pipe 6 to perform filtration and primary separation on the protein.