阅读说明：本技术 一种生物活性料液在低温下进行高倍浓缩的方法 (Method for high-power concentration of bioactive feed liquid at low temperature ) 是由 张亮 黄周金 于明晓 赵伟学 宋彦卓 姚立兵 于 2020-12-09 设计创作，主要内容包括：本发明公开了一种生物活性料液在低温下进行高倍浓缩的方法,通过将预制的生物活性稀料液进行冷冻定形后进行料冰分离,然后气吹或真空抽料使冷冻定形后的冰块完全变白,并收集得到高倍浓缩的生物活性料液,在低温条件下完成,即温度≤0℃即可完成浓缩,且浓缩效率高,一次性可实现6倍以上的浓缩倍数。同时,浓缩过程不需要复杂的设备和操作步骤,对设备的要求较低,可在各种条件下完成浓缩过程,为生物活性成分提供了一种新的浓缩途径,使用范围较广,适用于水溶性生物活性分子：包括大分子蛋白质、多糖类、肽类等均适用。(The invention discloses a method for high-power concentration of bioactive feed liquid at low temperature, which comprises freezing and shaping the prepared bioactive thin feed liquid, separating the material from ice, blowing or pumping the material in vacuum to make the ice white completely, collecting the bioactive feed liquid, and concentrating at low temperature (not higher than 0 deg.C), with high concentration efficiency, and concentration multiple of more than 6 times can be realized at one time. Meanwhile, the concentration process does not need complex equipment and operation steps, has low requirements on the equipment, can finish the concentration process under various conditions, provides a new concentration way for bioactive components, has wide application range, and is suitable for water-soluble bioactive molecules: including macromolecular proteins, polysaccharides, peptides, etc.)

1. A method for high-power concentration of bioactive feed liquid at low temperature is characterized in that: freezing and shaping the prepared bioactive thin liquid, separating the material from ice, blowing or vacuumizing to whiten the frozen and shaped ice blocks, and collecting the bioactive liquid with the concentration multiple of more than or equal to 6.

2. The method of claim 1 for high concentration of bioactive feed solution at low temperature, wherein: the freezing and shaping method comprises the specific steps of filling feed liquid into a container for freezing, so that the feed liquid is in a frozen state in a funnel.

3. The method of claim 2, wherein the bioactive feed solution is concentrated at low temperature by a high power method, comprising the steps of: the freezing temperature is-10 ℃ to-18 ℃, and the freezing time is 24h to 48 h.

4. The method of claim 2, wherein the bioactive feed solution is concentrated at low temperature by a high power method, comprising the steps of: the container is a device with a freezing chamber at the upper end and a feed liquid collecting chamber at the lower end, and a valve is arranged at the lower end of the freezing chamber.

5. The method of claim 4 for high concentration of bioactive feed solution at low temperature, wherein: feed liquid is collected the room lower extreme and is equipped with the feed opening, and the upper end side position is equipped with the evacuation mouth.

6. The method of claim 1 for high concentration of bioactive feed solution at low temperature, wherein: the bioactive material liquid is one or more of non-denatured collagen, chondroitin sulfate and collagen peptide.

7. The method of claim 1 for high concentration of bioactive feed solution at low temperature, wherein: the vacuum degree of the vacuum pumping is less than or equal to-0.1 MPa.

Technical Field

The invention belongs to the technical field of biotechnology, and particularly relates to a method for high-power concentration of bioactive feed liquid at low temperature.

Background

The application of bioactive components is more and more extensive in the field of human health, and the active components are generally sensitive to temperature, and the production process is required to be carried out under a low-temperature condition so as to ensure that the active structure of the product is not damaged, so that the active components of the product are retained to the greatest extent, and the sensory properties of the product are not changed.

At present, the concentration technology aiming at organic matters is widely applied by a membrane separation concentration technology or an evaporation concentration technology. The membrane separation concentration technology is widely applied in laboratory analysis and industrial mass production processes, but both an ultrafiltration unit and a nanofiltration unit are high in manufacturing cost, the service life of a membrane core is generally short (1-2 years), the operation temperature generally exceeds 30 ℃, great limitation is realized when heat-sensitive substances are concentrated, the concentration efficiency is continuously reduced along with the concentration, and the one-time concentration multiple is small and generally 3-4 times; the evaporation concentration technology is widely applied to vacuum type external circulation evaporation (single effect, double effect and triple effect) and vacuum type film evaporation (rising film and falling film), but the evaporation temperature is over 50 ℃, the evaporation concentration technology is not suitable for the concentration production of heat-sensitive substances, the concentration efficiency is low, and the one-time concentration multiple is small, generally 3-4 times.

Disclosure of Invention

In order to overcome the defects of the prior art, a novel concentration mode of the bioactive feed liquid is provided by utilizing freezing and vacuumizing equipment.

The invention provides a method for high-power concentration of bioactive feed liquid at low temperature, which comprises the steps of freezing and shaping a prefabricated bioactive thin feed liquid, then separating material from ice, blowing air or pumping the material in vacuum to completely whiten ice blocks after freezing and shaping, and collecting the high-power concentrated bioactive feed liquid.

Further, the freezing and shaping steps are that the feed liquid is filled in a container to be frozen, so that the feed liquid is in a frozen state in the funnel.

Further, the freezing temperature is-10 ℃ to-18 ℃, and the freezing time is 24h to 48 h.

Furthermore, the container is a device with a freezing chamber at the upper end and a feed liquid collecting chamber at the lower end, and a valve is arranged at the lower end of the freezing chamber.

Furthermore, feed liquid collection room lower extreme is equipped with the feed opening, and the upper end side position is equipped with the evacuation mouth.

Further, the bioactive material liquid is one or more of non-denatured collagen, chondroitin sulfate and collagen peptide.

Further, the vacuum degree of the vacuum pumping is less than or equal to-0.1 MPa.

Compared with the prior art, the invention has the advantages and the technical effects that: the concentration can be finished under the condition of low temperature, namely the temperature is less than or equal to 0 ℃, the concentration efficiency is high, and the concentration multiple of more than 6 times can be realized at one time. Meanwhile, the concentration process does not need complicated equipment and operation steps, has low requirements on the equipment and can be completed under various conditions; the temperature is lower in the treatment process, the activity of the bioactive feed liquid is ensured to the greatest extent, a new concentration way is provided for bioactive components, and the application range is wider: suitable for water-soluble bioactive molecules: including macromolecular proteins (such as non-denatured collagen), polysaccharides (such as chondroitin sulfate), peptides (such as collagen peptide), etc.

Drawings

Fig. 1 is a schematic structural diagram of a bioactive feed liquid concentrating container according to the present invention.

FIG. 2 is an electron micrograph of non-denatured collagen after concentration according to example 1 of the present invention.

Detailed Description

The technical solution of the present invention will be described in further detail with reference to the accompanying drawings and the detailed description.

The invention adopts the following process steps: prefabricated dilute liquid → freeze setting (-10 ℃ to-18 ℃, 24h to 48h) → material ice separation (compressed air blowing or vacuum system pumping) → concentrated liquid collection.

The container of concentrated bioactive feed liquid of preparation is a kind of upper end and is freezing chamber 1, and the lower extreme is equipped with the equipment that feed liquid collection room 2, and the upper end of freezing chamber 1 is equipped with material loading mouth 11, and 1 lower extreme of freezing chamber is equipped with valve 3, and 2 lower extremes of feed liquid collection room are equipped with feed opening 22, and the upper end side position is equipped with evacuation mouth 21 for collect indoor feed liquid evacuation to the feed liquid.

Regarding the selection of freezing temperature: 1) the non-modified collagen, the chondroitin sulfate and the collagen peptide can not be coagulated at the temperature of-10 ℃ and-18 ℃; 2) the risk of deterioration and microbial contamination of the product during long-time storage can be increased due to overhigh temperature, and the difficulty in separating the materials from the ice can be caused by overlow temperature; therefore, a temperature of-10 ℃ to-18 ℃ is the optimum separation temperature. The specific embodiment is as follows:

EXAMPLE 1 preparation of non-denatured collagen

1) Feed liquid prefabrication: 1kg of non-denatured collagen dilute liquid is prepared, and the content of the initial effective components is 1.56 percent;

2) freezing: the feed liquid is put into a container (with a pore canal with a valve at the lower end) for freezing, the freezing temperature is adjusted to-18 ℃, and the freezing time is 48 hours. The feed liquid is made to present a certain stable shape in the funnel, i.e. frozen state. (ii) a

3) Material ice separation: opening a valve at the lower end of the container and starting to collect the concentrated solution; pumping material from the lower end of the container under vacuum of-0.1 MPa for 10-15min, and stopping collecting concentrated solution until the ice cake is completely whitened.

4) Sampling the concentrated solution, detecting the content of effective constituent at 10.33%, and the concentration multiple at 6.6 times, and electron microscope image is shown in FIG. 2.

EXAMPLE 2 preparation of chondroitin sulfate

1) Feed liquid prefabrication: 1kg of chondroitin sulfate dilute solution is prepared, and the content of the initial effective components is 2.36%;

2) freezing: freezing the feed liquid in a container (with a pore channel with a valve at the lower end), adjusting the freezing temperature to-10 deg.C, and making the feed liquid present a certain stable shape in the funnel;

3) material ice separation: opening a valve at the lower end of the container and starting to collect the concentrated solution; and (3) pumping the material from the lower end of the container for 10-15min under the vacuum degree of-0.1 MPa, pumping out all the material liquid in the ice blocks until the ice blocks are completely whitened, and stopping collecting the concentrated solution.

4) Sampling the concentrated solution, and detecting that the content of the effective components is 18.01 percent and the concentration multiple is 7.6 times.

Example 3 preparation of collagen peptide

1) Feed liquid prefabrication: 1kg of collagen peptide dilute liquid is prepared, and the content of the initial effective components is 3.31 percent;

2) freezing: freezing the feed liquid in a container (with a pore channel with a valve at the lower end), adjusting the freezing temperature to-10 deg.C, and making the feed liquid present a certain stable shape in the funnel;

3) material ice separation: opening a valve at the lower end of the container and starting to collect the concentrated solution; and (3) pumping the material from the lower end of the container for 10-15min under the vacuum degree of-0.2 MPa, pumping out all the material liquid in the ice blocks until the ice blocks are completely whitened, and stopping collecting the concentrated solution.

4) Sampling the concentrated solution, and detecting that the content of the effective components is 26.54 percent and the concentration multiple is 8.0 times.

The concentration is carried out below 0 ℃, so that the soluble substance sensitive to temperature can be completely applicable, common bioactive feed liquid is carried out at high temperature, and the activity of the bioactive feed liquid can be damaged at the high temperature; the concentration can be realized without expensive and complicated special equipment, and the dependence of low-temperature concentration on equipment is solved; the concentration efficiency is high, high-power concentration can be realized at one time, a new concentration way is provided for bioactive components, the application range is wide, and the method is suitable for water-soluble bioactive molecules: including macromolecular proteins (such as non-denatured collagen), polysaccharides (such as chondroitin sulfate), peptides (such as collagen peptide), etc.

In addition, in the description of the present invention, it is to be understood that the terms "central," "upper," "lower," "front," "rear," "left," "right," "vertical," "horizontal," "top," "bottom," "inner," "outer," "axial," "radial," "circumferential," and the like are used in an orientation or positional relationship indicated for convenience in describing the present invention and to simplify description, but do not indicate or imply that the device or element so referred to must have a particular orientation, be constructed and operated in a particular orientation, and thus should not be construed as limiting the present invention.

The above description is only an example of the present invention, and is not intended to limit the present invention in any way, and those skilled in the art can make many variations and modifications of the present invention without departing from the scope of the present invention by using the method disclosed above, and the present invention is covered by the claims.