阅读说明：本技术 一种腺苷蛋氨酸发酵液破壁提取的方法 (Method for extracting ademetionine fermentation liquor by breaking cell wall ) 是由 金丹甜 付静 李明青 吴东奇 游云龙 于 2020-08-14 设计创作，主要内容包括：本发明公开了一种腺苷蛋氨酸发酵液破壁提取的方法。该腺苷蛋氨酸发酵液破壁提取的方法包括将酿酒酵母在发酵罐中培养30-80h,期间补加葡萄糖及L-蛋氨酸,直接将发酵液用于后提取；将酵母发酵液通入陶瓷膜中；经过浓缩后；菌浓浓度在30％～60％；将高菌浓浓度料液补水继续浓缩至透析液无色透明,将有机溶剂添加至陶瓷膜中,循环20-50min,继续浓缩取清液得抽提液；其中,有机溶剂与含固量比为1:1.3～1.8。本发明具有步骤简单,对设备的要求偏低,大大节约人力、物力、生产周期等生产成本,且提取收率高,有效提高了产品利润的效果。(The invention discloses a method for extracting adenosine methionine fermentation liquor by breaking cell walls. The method for extracting the adenosine methionine fermentation liquor by breaking the wall comprises the steps of culturing saccharomyces cerevisiae in a fermentation tank for 30-80 hours, supplementing glucose and L-methionine during the culture period, and directly using the fermentation liquor for post-extraction; introducing yeast fermentation liquor into the ceramic membrane; after concentration; the concentration of the bacteria is 30-60%; adding water into the high-bacteria-concentration feed liquid, continuously concentrating until the dialysate is colorless and transparent, adding an organic solvent into the ceramic membrane, circulating for 20-50min, and continuously concentrating to obtain a clear liquid to obtain an extract; wherein the ratio of the organic solvent to the solid content is 1: 1.3-1.8. The method has the advantages of simple steps, low requirement on equipment, great saving of production cost such as manpower, material resources and production period, high extraction yield and effective improvement of product profit.)

1. A method for extracting ademetionine fermentation liquor by breaking cell walls is characterized by comprising the following steps:

s1, culturing the saccharomyces cerevisiae in a fermentation tank for 30-80h, supplementing glucose and L-methionine during the culture, and directly using the fermentation liquor for post-extraction;

s2, introducing the yeast fermentation liquor into the ceramic membrane; after concentration; the concentration of the bacteria is 30-60%;

s3, adding water into the high-bacteria-concentration feed liquid, continuously concentrating until the dialysate is colorless and transparent, adding the organic solvent into the ceramic membrane, circulating for 20-50min, and continuously concentrating to obtain clear liquid to obtain an extract; wherein the ratio of the organic solvent to the solid content is 1: 1.3-1.8.

2. The method for extracting ademetionine fermentation broth by wall breaking according to claim 1, wherein the Saccharomyces cerevisiae (ATCC11909) is Saccharomyces cerevisiae in step S1.

3. The method for wall-breaking extraction of ademetionine fermentation broth according to claim 2, wherein the pore size of the ceramic membrane in step S2 is 8-100 nm.

4. The method for wall-broken extraction of ademetionine fermentation broth according to claim 1, wherein the standard of colorless in step S3 is below standard colorimetric solution Y-3.

5. The method for breaking the wall of the adenosyl methionine fermentation broth for extraction according to claim 1, wherein the solvent in step S3 is an ester solvent or a ketone solvent.

6. The method for wall-broken extraction of ademetionine fermentation liquor according to claim 1, wherein the solid content in step S3 is the product of the total volume of the fermentation liquor and the microbial concentration of the fermentation liquor.

7. The method for wall-breaking extraction of ademetionine fermentation broth according to claim 1, wherein in step S3, the high-concentration feed liquid is supplemented with water and continuously concentrated until the dialysate is colorless and transparent, the organic solvent is added into the ceramic membrane, the circulation is carried out for 20-25min, and the supernatant is continuously concentrated to obtain an extract; wherein the ratio of the organic solvent to the solid content is 1: 1.3-1.5.

8. The method for wall-breaking extraction of ademetionine fermentation broth according to claim 5, wherein said ester solvent and ketone solvent are ethyl acetate, acetone or butanone.

Technical Field

The invention relates to the technical field of biological pharmacy, in particular to a method for extracting adenosine methionine fermentation liquor by breaking walls.

Background

S-Adenosyl-L-methionine (SAM) was discovered in 1953 as a sulfonyl compound in the form of an organic quaternary sulfur of methionine. It is widely present in various organisms and is an important physiologically active substance in the human body. Biologically, SAM is a one-, three-and five-carbon group donor, with methyl-, propyl-, and sulfur-transfer physiological effects.

Its chemical name is "+/-5 '- [ (R) -3-amino-3-carboxypropyl ] methylsulfonyl ] -5' -deoxyadenosine 1, 4-butanedisulfonate"

English name ± -5'- [ (R) -, 3-amino-3-carboxypropyl ] methysulfonio ] -5' -deoxysylnine inner salt,1, 4-butandisultaine

Chemical structure

At present, the domestic demand for 1, 4-butanedisulfonic acid adenosine methionine is large, but the productivity is insufficient, and under the condition based on the prior art, the extraction mode with high yield and simple operation on the adenosine methionine fermentation liquor needs to be researched and invented.

Disclosure of Invention

According to one aspect of the invention, the method for extracting the ademetionine fermentation liquor by breaking the wall comprises the following steps:

s1, culturing the saccharomyces cerevisiae in a fermentation tank for 30-80h, supplementing glucose and L-methionine during the culture, and directly using the fermentation liquor for post-extraction;

s2, introducing the yeast fermentation liquor into the ceramic membrane; after concentration; the concentration of the bacteria is 30-60%;

s3, adding water into the high-bacteria-concentration feed liquid, continuously concentrating until the dialysate is colorless and transparent, adding the organic solvent into the ceramic membrane, circulating for 20-50min, and continuously concentrating to obtain clear liquid to obtain an extract; wherein the ratio of the organic solvent to the solid content is 1: 1.3-1.8.

In some embodiments, the Saccharomyces cerevisiae in step S1 is Saccharomyces cerevisiae (ATCC 11909).

In some embodiments, the ceramic membrane in step S2 has a pore size of 8-100 nm.

In some embodiments, the colorless standard in step S3 is less than or equal to standard colorimetric solution Y-3.

In some embodiments, the solvent in step S3 is an ester solvent or a ketone solvent.

In some embodiments, the solid content in step S3 is the product of the total volume of the fermentation broth and the microbial concentration of the fermentation broth.

In some embodiments, in step S3, the high-bacteria-concentration feed liquid is further concentrated until the dialysate is colorless and transparent, the organic solvent is added into the ceramic membrane, the circulation is performed for 20-25min, and the clear liquid is further concentrated to obtain an extract; wherein the ratio of the organic solvent to the solid content is 1: 1.3-1.5.

In some embodiments, the ester solvent and ketone solvent in step S3 are ethyl acetate, acetone or butanone.

The method for extracting the adenosine methionine fermentation liquor by breaking the wall has the advantages that the fermentation liquor of the adenosine methionine producing bacteria is subjected to ceramic membrane filtration to obtain high-bacteria-concentration fermentation liquor; adding organic solvent solution, circulating, filtering and collecting filtrate. The method has simple steps, low requirements on equipment, high extraction yield and the like, greatly saves the production cost of manpower, material resources, production period and the like, and effectively improves the product profit.

Detailed Description

The present invention will be described in further detail with reference to examples.