阅读说明：本技术 一种山药黏液—纳米氧化锌溶液及其制备方法和应用 (Chinese yam mucilage-nano zinc oxide solution and preparation method and application thereof ) 是由 马凡怡 张昀 李晓静 刘绣华 蔡延新 白洁 于 2020-05-27 设计创作，主要内容包括：本发明属于抗菌、保鲜剂领域,特别是指一种山药黏液—纳米氧化锌溶液及其制备方法和应用。将山药黏液加入纳米氧化锌分散液中,制备的溶液具有抗菌作用,该溶液环保,无毒无害,可快速降解,可以用于食品保鲜,符合绿色化学的要求。本发明首先提取山药黏液,然后加入不同比例的山药黏液制成不同比例的山药黏液—纳米氧化锌溶液。本方法制备的五种山药黏液—纳米氧化锌溶液具有很好的水溶性,分散性,溶液具有抗菌作用,能够延长食品的保质期,制备的溶液在食品、医疗等领域有广泛的应用前景。(The invention belongs to the field of antibacterial and preservative agents, and particularly relates to a yam mucilage-nano zinc oxide solution as well as a preparation method and application thereof. The yam mucilage is added into the nano zinc oxide dispersion liquid, the prepared solution has an antibacterial effect, is environment-friendly, non-toxic and harmless, can be rapidly degraded, can be used for food preservation, and meets the requirement of green chemistry. The invention firstly extracts yam mucilage, and then adds yam mucilage with different proportions to prepare yam mucilage-nano zinc oxide solution with different proportions. The five Chinese yam mucilages-nano zinc oxide solution prepared by the method has good water solubility and dispersibility, the solution has an antibacterial effect, the shelf life of food can be prolonged, and the prepared solution has wide application prospects in the fields of food, medical treatment and the like.)

1. A preparation method of a yam mucilage-nano zinc oxide solution is characterized by comprising the following steps:

(1) extracting Chinese yam mucilage: cleaning rhizoma Dioscoreae with clear water, air drying until the surface is anhydrous, peeling, cutting into uniform cylindrical blocks, adding appropriate amount of distilled water into rhizoma Dioscoreae, stirring to obtain slurry, centrifuging, and collecting supernatant to obtain mucus DOM;

(2) preparing yam mucilage-nano zinc oxide solution: and (2) adding the nano zinc oxide dispersion liquid into a beaker, then sequentially adding distilled water and glycerol, placing the mixture into a water bath kettle, then adding carboxymethyl cellulose (CMC), stirring the mixture while adding the CMC until the mixture is uniform, then adding the viscous DOM extracted in the step (1) in proportion, and stirring the mixture uniformly to obtain CMC-ZnO, CMC-ZnO-DOM1, CMC-ZnO-DOM2 or CMC-ZnO-DOM3 solution.

2. The method for preparing yam mucilage-nano zinc oxide solution according to claim 1, which is characterized in that: the addition mass volume ratio w/v of the Chinese yam and the distilled water in the step (1) is (2-10): (60-100).

3. The method for preparing yam mucilage-nano zinc oxide solution according to claim 1, which is characterized in that: in the step (2), the mass ratio w/w of the nano zinc oxide, the CMC and the mucus DOM is 2: (1-1.4): (0.6-1).

4. The method for preparing yam mucilage-nano zinc oxide solution according to claim 1, which is characterized in that: the temperature of the water bath kettle in the step (2) is 40-60 ℃.

5. The yam mucilage-nano zinc oxide solution prepared by the method of any one of claims 1-4, which is characterized in that: the yam mucilage-nano zinc oxide solution is CMC-ZnO, CMC-ZnO-DOM1, CMC-ZnO-DOM2 or CMC-ZnO-DOM3 solution.

6. The use of the yam mucilage-nano zinc oxide solution of claim 5 for prolonging the shelf life of food and medicine.

7. The use of the yam mucilage-nano zinc oxide solution of claim 5 for inhibiting the orientation of escherichia coli and staphylococcus aureus.

Technical Field

The invention belongs to the field of antibacterial and preservative agents, and particularly relates to a yam mucilage-nano zinc oxide solution as well as a preparation method and application thereof.

Background

With the improvement of living standard of people, environmental protection and health are more and more important, the production and consumption of global plastics continue to increase, wherein a great part of plastics produced every year are used for packaging food. Since such materials are not biodegradable, serious problems have been caused, such as environmental hazards. The green degradable package is sought to replace plastic products and improve the environment. The plant mucus is used as a food hydrocolloid, has good film forming potential, and has the advantages of no toxicity, low cost, no irritation and the like. Rhizoma Dioscoreae is a Dioscoreaceae plant, its tuber has various medicinal values, and rhizoma Dioscoreae mucilage has effects of reducing blood lipid and resisting bacteria. When the nanomaterial is added to a compatible polymer, the properties of the nanocomposite produced can be greatly improved. Patent CN201610672416.7 discloses an antibacterial food packaging material with food preservation function and a manufacturing method thereof, and the patent mentions that nano zinc oxide activated by a silane coupling agent is used as a photocatalytic antibacterial agent and is compatible with other various chemical components to prepare the antibacterial food packaging material for preservation. However, the packaging material cannot be directly and tightly combined with food, and the fresh-keeping effect is common; the invention mainly adds the yam mucilage into the nano zinc oxide dispersion liquid, the prepared solution has an antibacterial effect, and the solution is environment-friendly, non-toxic, harmless, rapidly degradable, capable of being used for food preservation and meeting the requirement of green chemistry.

Disclosure of Invention

The invention provides a yam mucilage-nano zinc oxide solution and a preparation method and application thereof.

The technical scheme of the invention is realized as follows:

a preparation method of a yam mucilage-nano zinc oxide solution comprises the following steps:

(1) extracting Chinese yam mucilage: cleaning rhizoma Dioscoreae with clear water, air drying until the surface is anhydrous, peeling, cutting into uniform cylindrical blocks, adding appropriate amount of distilled water into rhizoma Dioscoreae, stirring to obtain slurry, centrifuging, and collecting supernatant to obtain mucus DOM;

(2) preparing yam mucilage-nano zinc oxide solution: and (2) adding the nano zinc oxide dispersion liquid into a beaker, then sequentially adding distilled water and glycerol, placing the mixture into a water bath kettle, then adding carboxymethyl cellulose (CMC), stirring the mixture while adding until the mixture is uniform, then adding the viscous liquid DOM extracted in the step (1) in proportion, and stirring the mixture uniformly to obtain CMC-ZnO, CMC-ZnO-DOM1, CMC-ZnO-DOM2 or CMC-ZnO-DOM3 solutions.

The addition mass volume ratio w/v of the Chinese yam and the distilled water in the step (1) is (2-10): (60-100).

In the step (2), the mass ratio w/w of the nano zinc oxide, the CMC and the mucus DOM is 2: (1-1.4): (0.6-1)

The temperature of the water bath kettle in the step (2) is 40-60 ℃.

The yam mucilage-nano zinc oxide solution prepared by the method is CMC-ZnO, CMC-ZnO-DOM1, CMC-ZnO-DOM2 or CMC-ZnO-DOM 3.

The yam mucilage-nano zinc oxide solution is applied to prolonging the shelf life of food and medicines.

The yam mucilage-nano zinc oxide solution is used for inhibiting escherichia coli: (escherichia coli)E.coil) And Staphylococcus aureus (S.aureus) The application of the direction.

The invention has the following beneficial effects:

1. the method has simple operation and short reaction time, and the prepared five solutions have good dispersibility in the aqueous solution. The prepared yam mucilage-zinc oxide solution can be used for food preservation, antibiosis and the like.

2. Five solutions of CMC, CMC-ZnO-DOM1, CMC-ZnO-DOM2 and CMC-ZnO-DOM3 are prepared, wherein the solutions of CMC-ZnO, CMC-ZnO-DOM1, CMC-ZnO-DOM2 and CMC-ZnO-DOM3 have high antibacterial ability, and can be used for treating staphylococcus aureus (staphylococcus aureus)S.aureus) And Escherichia coli (E. coil) Has antibacterial effect.

Drawings

In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings used in the description of the embodiments or the prior art will be briefly described below, it is obvious that the drawings in the following description are only some embodiments of the present invention, and for those skilled in the art, other drawings can be obtained according to the drawings without creative efforts.

FIG. 1 is the SEM images of five solutions of CMC (A), CMC-ZnO (B), CMC-ZnO-DOM1 (C), CMC-ZnO-DOM2 (D) and CMC-ZnO-DOM3 (E) obtained in example 1.

FIG. 2 is an infrared spectrum of five solutions CMC (A), CMC-ZnO (B), CMC-ZnO-DOM1 (C), CMC-ZnO-DOM2 (D) and CMC-ZnO-DOM3 (E) obtained in example 1.

FIG. 3 is a thermogravimetric plot of five solutions, CMC (A), CMC-ZnO (B), CMC-ZnO-DOM1 (C), CMC-ZnO-DOM2 (D), CMC-ZnO-DOM3 (E), obtained in example 1.

FIG. 4 shows the results of example 1 for five solutions of CMC (A), CMC-ZnO (B), CMC-ZnO-DOM1 (C), CMC-ZnO-DOM2 (D), CMC-ZnO-DOM3 (E) against E.coli (E)E. coil) The antibacterial pattern of (1).

FIG. 5 shows five solutions of CMC (A), CMC-ZnO (B), CMC-ZnO-DOM1 (C), CMC-ZnO-DOM2 (D), CMC-ZnO-DOM3 (E) obtained in example 1 against Staphylococcus aureus (S.), (B)S.aureus) The antibacterial pattern of (1).

FIG. 6 is a graph of weight loss rate of five film-coated apples for 15 days.

Detailed Description

The technical solutions of the present invention will be described clearly and completely with reference to the following embodiments of the present invention, and it should be understood that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be obtained by a person skilled in the art without inventive effort based on the embodiments of the present invention, are within the scope of the present invention.

A preparation method of a yam mucilage-nano zinc oxide solution comprises the following steps:

(1) extracting Chinese yam mucilage: cleaning rhizoma Dioscoreae with clear water, air drying until the surface is anhydrous, peeling, cutting into uniform cylindrical blocks, adding appropriate amount of distilled water into rhizoma Dioscoreae, stirring to obtain slurry, centrifuging, and collecting supernatant to obtain mucus DOM;

(2) preparing yam mucilage-nano zinc oxide solution: and (2) adding the nano zinc oxide dispersion liquid into a beaker, then sequentially adding distilled water and glycerol, placing the mixture into a water bath kettle, then adding CMC, stirring the mixture uniformly while adding, adding the mucus DOM extracted in the step (1) in proportion, and stirring the mixture uniformly to obtain CMC-ZnO, CMC-ZnO-DOM1, CMC-ZnO-DOM2 or CMC-ZnO-DOM3 solution.

The addition mass volume ratio w/v of the Chinese yam and the distilled water in the step (1) is 2-10: 60-100.

In the step (2), the mass ratio w/w of the nano zinc oxide, the CMC and the mucus DOM is 2: (1-1.4): (0.6-1)

The temperature of the water bath kettle in the step (2) is 40-60 ℃.

The yam mucilage-nano zinc oxide solution prepared by the method is CMC-ZnO, CMC-ZnO-DOM1, CMC-ZnO-DOM2 or CMC-ZnO-DOM 3.

The yam mucilage-nano zinc oxide solution is applied to prolonging the shelf life of food and medicines.

The yam mucilage-nano zinc oxide solution is used for inhibiting escherichia coli: (escherichia coli)E.coil) And Staphylococcus aureus (S.aureus) The application of the direction.