阅读说明：本技术 包含工程化嵌合抗原受体和car调节剂的组合物和方法 (Compositions and methods comprising engineered chimeric antigen receptors and CAR modulators ) 是由 M.普勒 V.佩达雷迪加里 C.伊汀 于 2020-03-06 设计创作，主要内容包括：提供了一种用于制备细胞组合物的方法,所述方法包括用至少两种病毒载体的混合物转导细胞群的步骤,其中至少一种载体包含编码嵌合抗原受体(CAR)的核酸序列；且其中至少一种载体包含编码活性调节剂的核酸,所述活性调节剂调节CAR的活性、表达CAR的细胞的活性或靶细胞的活性。还提供了通过此类方法制备的细胞组合物及其在治疗疾病如癌症中的用途。(A method for preparing a cell composition is provided, the method comprising the steps of transducing a population of cells with a mixture of at least two viral vectors, wherein at least one vector comprises a nucleic acid sequence encoding a Chimeric Antigen Receptor (CAR); and wherein at least one vector comprises a nucleic acid encoding a modulator of activity that modulates the activity of the CAR, the activity of a cell expressing the CAR, or the activity of a target cell. Also provided are cell compositions prepared by such methods and their use in treating diseases such as cancer.)