阅读说明：本技术 铁皮石斛解酒保肝组合物和制法 (Dendrobium officinale alcohol-dispelling and liver-protecting composition and preparation method thereof ) 是由 梁斌 张丽艳 唐靖雯 杨槐 卢礼平 潘梅 彭政忠 唐先博 南美花 蒲翔 李洲 于 2019-11-18 设计创作，主要内容包括：本发明涉及铁皮石斛解酒保肝组合物和制法。具体地说,所述铁皮石斛解酒保肝组合物是由铁皮石斛叶、葛根皮和玉米低聚肽制备得到的,该组合物中包含铁皮石斛叶浸提液10～20份、铁皮石斛叶发酵液5～10份、葛根皮发酵液10～30份、玉米低聚肽粉0.2～0.6份。本发明还涉及该组合物的制备方法,包括如下步骤：将铁皮石斛叶浸提液、铁皮石斛叶发酵液、葛根皮发酵液混合均匀,加入玉米低聚肽粉使溶解,任选的加入调味剂溶解,和/或任选的加水稀释,得到组合物。本发明组合物具有优异的解酒和保肝的效果。(The invention relates to a dendrobium officinale alcohol-effect-dispelling and liver-protecting composition and a preparation method thereof. Specifically, the dendrobium officinale alcohol-effect-dispelling and liver-protecting composition is prepared from dendrobium officinale leaves, pueraria lobata peels and corn oligopeptide, and the composition comprises 10-20 parts of dendrobium officinale leaf leaching liquor, 5-10 parts of dendrobium officinale leaf fermentation liquor, 10-30 parts of pueraria lobata peel fermentation liquor and 0.2-0.6 part of corn oligopeptide powder. The invention also relates to a preparation method of the composition, which comprises the following steps: mixing the leaching solution of Dendrobium officinale leaf, fermentation broth of Dendrobium officinale leaf and fermentation broth of Pueraria lobata skin, adding corn oligopeptide powder for dissolving, optionally adding flavoring agent for dissolving, and/or optionally adding water for diluting to obtain the composition. The composition has excellent effects of relieving alcoholism and protecting liver.)

1. Dendrobium officinale alcohol-effect-dispelling and liver-protecting composition, which is prepared from Dendrobium officinale leaves, kudzu root bark and corn oligopeptide.

2. The dendrobium officinale alcohol effect relieving and liver protecting composition according to claim 1, wherein:

the dendrobium officinale leaves are added into the hangover alleviating composition in the form of dendrobium officinale leaf leaching liquor and dendrobium officinale leaf fermentation liquor; and/or

The pueraria lobata peel is added into the anti-alcoholism composition in the form of pueraria lobata peel fermentation liquor.

3. The dendrobium officinale alcohol effect relieving and liver protecting composition according to claim 1, wherein:

the dendrobium officinale leaf leaching liquor is prepared by the following method:

(a1) withering and rolling: spreading and airing the freshly picked dendrobium officinale leaves, placing the leaves in a dark and ventilated place for naturally withering for 8-10 h, then airing to enable the fresh leaves to be in a wilted state, separating old and tender leaves in a rolling chamber with the temperature of 20-24 ℃ and the humidity of 85-90%, slightly pressing and short rolling the tender leaves, rolling the old leaves by a medium-pressure hairpin, uniformly rolling, and rolling the leaves into strips;

(a2) fermentation: putting the rolled dendrobium officinale leaves into a fermentation frame, putting the dendrobium officinale leaves into a fermentation chamber for fermentation, keeping the temperature of the fermented leaves at 27-30 ℃, and spreading the leaves to be 5-15 cm thick;

(a3) and (3) drying: drying the fermented leaves at 70-80 ℃ until the water content is 15-20%, spreading for cooling, continuously baking at the high temperature of 95-105 ℃ until the water content of the fermented leaves is 3-8%, and crushing into powder with the size of 0.5mm³Crushing the rice;

(a4) leaching: adding 10-50 times of water by weight into the obtained fermented leaf particles, leaching at 85-100 ℃ for 30-60 min, then rapidly cooling to 20 ℃, and filtering through a 200-500-mesh sieve to obtain a dendrobium officinale leaf leaching liquor;

the dendrobium officinale leaf fermentation liquor is prepared by the following method:

(b1) activating strains: mixing the powder (containing Lactobacillus paracasei 3.0 × 10)¹⁰cfu/g, Lactobacillus acidophilus 1.5X 10¹⁰cfu/g and Lactobacillus plantarum 2.0X 10¹⁰cfu/g) is added into 10-20 times of sterile distilled water with the temperature of 35-40 ℃ for dissolving, and then the mixture is placed in a thermostat with the temperature of 37-39 ℃ for activation for 30min to obtain lactic acid bacteria liquid for later use;

(b2) preparing the dendrobium officinale leaf enzymatic hydrolysate: adding freshly picked dendrobium officinale leaves into water with the weight 5 times that of the leaves to perform cell wall breaking treatment, so that the wall breaking rate reaches more than 60%, then pre-boiling the leaves at 85-95 ℃ for 10-30 min, then rapidly cooling the leaves to 45-55 ℃, adjusting the pH value to 4.5-5.0, adding a complex enzyme (containing 16000U/g pectinase and 30000U/g cellulase) at the concentration of 0.04-0.08 g/L, placing the complex enzyme in a water bath kettle at 45-55 ℃, performing enzymolysis for 1-2 h, and inactivating the enzyme to obtain an enzymolysis solution of the dendrobium officinale;

(b3) preparing a dendrobium officinale leaf fermentation liquid: adding 2-5% of a nutritional aid (the nutritional aid is selected from glucose, fructose, xylose, mannose, galactose and erythrose) into the dendrobium officinale leaf enzymatic hydrolysate, sterilizing at 90-95 ℃ for 5min, and rapidly cooling to 35-40 ℃; adding activated lactobacillus liquid (the volume ratio of the enzymolysis liquid to the lactobacillus liquid is 100: 2-3), carrying out anaerobic fermentation at 35-40 ℃ for 16-72 h, immediately cooling to 2-10 ℃ when the pH of the fermentation liquid reaches 3.5-4.0, stopping fermentation, filtering with a 200-500-mesh sieve, and sterilizing the filtrate at 90-95 ℃ for 5min to obtain dendrobium officinale leaf fermentation liquid; and/or

The kudzuvine root bark fermentation liquor is prepared by the following method:

(c1) activating strains: obtaining lactic acid bacteria liquid for later use according to the method in the step (b 1);

(c2) preparation of pueraria lobata skin enzymolysis liquid: crushing kudzu root bark by using a crusher, sieving the crushed kudzu root bark by using a 100-mesh sieve, adding 10 times of water by weight, uniformly mixing, pulping to obtain kudzu root bark slurry, pre-boiling the slurry at 85-100 ℃ for 30-60 min to fully gelatinize the slurry to obtain gelatinized liquid, adjusting the pH to 5.0-6.5, adding high-temperature amylase (40000U/g) at the concentration of 0.1-0.5 g/L, placing the gelatinized liquid in a 90-95 ℃ water bath kettle, performing enzymolysis for 2-3 h, cooling to 50 ℃, adjusting the pH to 4.5-5.0, adding complex enzyme (the complex enzyme is a mixture of 40000U/g amylase and 30000U/g cellulase) at the concentration of 0.04-0.08 g/L, placing the gelatinized liquid in a 45-55 ℃ water bath kettle, performing enzymolysis for 1-2 h, and deactivating enzyme to obtain kudzu root bark enzymatic hydrolysate;

(c3) preparing a kudzuvine root bark fermentation liquid: adding 2-5% of a nutritional aid (the nutritional aid is selected from glucose, fructose, xylose, mannose, galactose and erythrose) into the pueraria lobata skin enzymolysis liquid, sterilizing at 90-95 ℃ for 5min, and rapidly cooling to 35-40 ℃; and adding activated lactobacillus liquid (the volume ratio of the enzymolysis liquid to the lactobacillus liquid is 100: 3-5), carrying out anaerobic fermentation for 16-72 h at 35-40 ℃, immediately cooling to 2-10 ℃ when the pH of the fermentation liquid reaches 3.5-4.0, stopping fermentation, filtering with a 200-500-mesh sieve, and sterilizing the filtrate at 90-95 ℃ for 5min to obtain the pueraria lobata skin fermentation liquid.

4. The dendrobium officinale alcohol effect relieving and liver protecting composition according to claim 1, wherein:

the dendrobium officinale leaf leaching liquor is prepared by the following method:

(a1) withering and rolling: spreading and airing the freshly picked dendrobium officinale leaves, placing the leaves in a dark and ventilated place for naturally withering for 8h, then airing to enable the fresh leaves to be in a wilted state, separating old and tender leaves in a rolling chamber with the temperature of 22 ℃ and the humidity of 85%, slightly pressing and short rolling tender leaves, rolling old leaves by a medium-pressure hairpin, uniformly rolling, and rolling the leaves into strips;

(a2) fermentation: putting the rolled dendrobium officinale leaves into a fermentation frame, putting the dendrobium officinale leaves into a fermentation chamber for fermentation, keeping the temperature of the fermented leaves at 28 ℃, and spreading the leaves with the thickness of 5 cm;

(a3) and (3) drying: drying the fermented leaves at 75 ℃ until the water content is 15%, spreading for cooling, continuously baking at a high temperature of 95 ℃ until the water content of the fermented leaves is 3-4%, and crushing into powder with the size of 0.5mm³Crushing the rice;

(a4) leaching: adding 10 times of water into the fermented leaf granules, leaching at 90 deg.C for 45min, rapidly cooling to 20 deg.C, and filtering with 200 mesh sieve to obtain herba Dendrobii leaf leaching solution;

the dendrobium officinale leaf fermentation liquor is prepared by the following method:

(b1) activating strains: mixing the powder (containing Lactobacillus paracasei 3.0 × 10)¹⁰cfu/g, Lactobacillus acidophilus 1.5X 10¹⁰cfu/g and Lactobacillus plantarum 2.0X 10¹⁰cfu/g) is added into 10 times of sterile distilled water with the temperature of 37 ℃ for dissolution, and then the mixture is placed in a thermostat with the temperature of 37 ℃ for activation for 30min to obtain lactic acid bacteria liquid for later use;

(b2) preparing the dendrobium officinale leaf enzymatic hydrolysate: adding freshly picked dendrobium officinale leaves into water with the weight 5 times that of the leaves to perform cell wall breaking treatment, so that the wall breaking rate reaches more than 60%, then pre-boiling for 20min at 90 ℃, then rapidly cooling to 50 ℃, adjusting the pH value to 4.5-5.0, adding a complex enzyme (containing 16000U/g pectinase and 30000U/g cellulase) at the concentration of 0.06g/L, placing the complex enzyme in a water bath kettle at 50 ℃, performing enzymolysis for 1.5h, and performing enzyme deactivation to obtain an enzymatic hydrolysate of the dendrobium officinale;

(b3) preparing a dendrobium officinale leaf fermentation liquid: adding 3% of nutritional adjuvants (selected from glucose, fructose, xylose, mannose, galactose and erythrose) into the enzymatic hydrolysate, sterilizing at 95 deg.C for 5min, and rapidly cooling to 37 deg.C; adding activated lactobacillus liquid (the volume ratio of the enzymolysis liquid to the lactobacillus liquid is 100: 2.5), carrying out anaerobic fermentation at 37 ℃ for 16-72 h, immediately cooling to 4 ℃ when the pH of the fermentation liquid reaches 3.5-4.0, stopping fermentation, filtering with a 200-mesh sieve, and sterilizing the filtrate at 95 ℃ for 5min to obtain dendrobium officinale leaf fermentation liquid; and/or

The kudzuvine root bark fermentation liquor is prepared by the following method:

(c1) activating strains: obtaining lactic acid bacteria liquid for later use according to the method in the step (b 1);

(c2) preparation of pueraria lobata skin enzymolysis liquid: crushing kudzuvine root bark by using a crusher, sieving the crushed kudzuvine root bark by using a 100-mesh sieve, adding 10 times of water by weight, uniformly mixing, pulping to obtain kudzuvine root bark pulp, pre-boiling the pulp at 90 ℃ for 45min to fully gelatinize the pulp to obtain gelatinized liquid, adjusting the pH to 5.0-5.5, adding high-temperature amylase (40000U/g) at the concentration of 0.25g/L, placing the gelatinized liquid in a 95 ℃ water bath kettle, performing enzymolysis for 2.5h, cooling to 50 ℃, adjusting the pH to 4.5-5.0, adding complex enzyme (the complex enzyme is a mixture of 40000U/g amylase and 30000U/g cellulase) at the concentration of 0.05g/L, placing the complex enzyme in a 50 ℃ water bath kettle, performing enzymolysis for 1.5h, and deactivating the enzyme to obtain kudzuvine root bark enzymatic hydrolysate;

(c3) preparing a kudzuvine root bark fermentation liquid: adding 3% of nutritional adjuvants (selected from glucose, fructose, xylose, mannose, galactose and erythrose) into the enzymatic hydrolysate of radix Puerariae peel, sterilizing at 95 deg.C for 5min, and rapidly cooling to 37 deg.C; and adding activated lactobacillus liquid (the volume ratio of the enzymolysis liquid to the lactobacillus liquid is 100: 3.5), carrying out anaerobic fermentation at 37 ℃ for 16-72 h, immediately cooling to 4 ℃ when the pH of the fermentation liquid reaches 3.5-4.0, stopping fermentation, filtering with a 200-mesh sieve, and sterilizing the filtrate at 95 ℃ for 5min to obtain the pueraria lobata skin fermentation liquid.

5. The dendrobium officinale alcohol effect relieving and liver protecting composition according to claim 1, wherein:

the paint comprises the following components in percentage by weight:

10-20 parts of dendrobium officinale leaf leaching liquor,

5-10 parts of dendrobium officinale leaf fermentation liquor,

10-30 parts of pueraria lobata peel fermentation liquor,

0.2-0.6 parts of corn oligopeptide powder;

the paint comprises the following components in percentage by weight:

15 parts of dendrobium officinale leaf leaching liquor,

8 parts of dendrobium officinale leaf fermentation liquor,

20 portions of pueraria root bark fermentation liquor,

0.4 part of corn oligopeptide powder;

the composition can be diluted with water, for example, the dendrobium officinale alcohol-relieving and liver-protecting composition comprises:

10-20% of dendrobium officinale leaf leaching liquor,

5-10% of dendrobium officinale leaf fermentation liquor,

10-30% of pueraria peel fermentation liquor,

0.2-0.6% of corn oligopeptide powder,

Adding water to 100%; and/or

The composition can be diluted with water, for example, the dendrobium officinale alcohol-relieving and liver-protecting composition comprises:

15 percent of dendrobium officinale leaf leaching liquor,

8 percent of dendrobium officinale leaf fermentation liquor,

20 percent of pueraria root bark fermentation liquor,

0.4 percent of corn oligopeptide powder,

Adding water to 100%.

6. The anti-hangover and hepatoprotective composition according to claim 1, further comprising a flavoring agent, such as sucrose, crystal sugar, lemon juice, such as lemon juice, etc.

7. A method for preparing the dendrobium officinale alcohol-effect-relieving and liver-protecting composition of any one of claims 1-6, which comprises the following steps:

(1) providing a leaching solution of dendrobium officinale leaves;

(2) providing a dendrobium officinale leaf fermentation liquid;

(3) providing pueraria lobata peel fermentation liquor;

(4) and (4) preparing the composition.

8. The method of claim 7, wherein:

the dendrobium officinale leaf leaching liquor is prepared by the following method:

(a1) withering and rolling: spreading and airing the freshly picked dendrobium officinale leaves, placing the leaves in a dark and ventilated place for naturally withering for 8-10 h, then airing to enable the fresh leaves to be in a wilted state, separating old and tender leaves in a rolling chamber with the temperature of 20-24 ℃ and the humidity of 85-90%, slightly pressing and short rolling the tender leaves, rolling the old leaves by a medium-pressure hairpin, uniformly rolling, and rolling the leaves into strips;

(a2) fermentation: putting the rolled dendrobium officinale leaves into a fermentation frame, putting the dendrobium officinale leaves into a fermentation chamber for fermentation, keeping the temperature of the fermented leaves at 27-30 ℃, and spreading the leaves to be 5-15 cm thick;

(a3) and (3) drying: drying the fermented leaves at 70-80 ℃ until the water content is 15-20%, spreading for cooling, and then continuously baking at the high temperature of 95-105 ℃ until the fermented leaves contain water3-8% of the total weight, and crushing the mixture into particles with the size of 0.5mm³Crushing the rice;

(a4) leaching: adding 10-50 times of water by weight into the obtained fermented leaf particles, leaching at 85-100 ℃ for 30-60 min, then rapidly cooling to 20 ℃, and filtering through a 200-500-mesh sieve to obtain a dendrobium officinale leaf leaching liquor;

the dendrobium officinale leaf fermentation liquor is prepared by the following method:

(b1) activating strains: mixing the powder (containing Lactobacillus paracasei 3.0 × 10)¹⁰cfu/g, Lactobacillus acidophilus 1.5X 10¹⁰cfu/g and Lactobacillus plantarum 2.0X 10¹⁰cfu/g) is added into 10-20 times of sterile distilled water with the temperature of 35-40 ℃ for dissolving, and then the mixture is placed in a thermostat with the temperature of 37-39 ℃ for activation for 30min to obtain lactic acid bacteria liquid for later use;

(b2) preparing the dendrobium officinale leaf enzymatic hydrolysate: adding freshly picked dendrobium officinale leaves into water with the weight 5 times that of the leaves to perform cell wall breaking treatment, so that the wall breaking rate reaches more than 60%, then pre-boiling the leaves at 85-95 ℃ for 10-30 min, then rapidly cooling the leaves to 45-55 ℃, adjusting the pH value to 4.5-5.0, adding a complex enzyme (containing 16000U/g pectinase and 30000U/g cellulase) at the concentration of 0.04-0.08 g/L, placing the complex enzyme in a water bath kettle at 45-55 ℃, performing enzymolysis for 1-2 h, and inactivating the enzyme to obtain an enzymolysis solution of the dendrobium officinale;

(b3) preparing a dendrobium officinale leaf fermentation liquid: adding 2-5% of a nutritional aid (the nutritional aid is selected from glucose, fructose, xylose, mannose, galactose and erythrose) into the dendrobium officinale leaf enzymatic hydrolysate, sterilizing at 90-95 ℃ for 5min, and rapidly cooling to 35-40 ℃; adding activated lactobacillus liquid (the volume ratio of the enzymolysis liquid to the lactobacillus liquid is 100: 2-3), carrying out anaerobic fermentation at 35-40 ℃ for 16-72 h, immediately cooling to 2-10 ℃ when the pH of the fermentation liquid reaches 3.5-4.0, stopping fermentation, filtering with a 200-500-mesh sieve, and sterilizing the filtrate at 90-95 ℃ for 5min to obtain dendrobium officinale leaf fermentation liquid; and/or

The kudzuvine root bark fermentation liquor is prepared by the following method:

(c1) activating strains: obtaining lactic acid bacteria liquid for later use according to the method in the step (b 1);

(c2) preparation of pueraria lobata skin enzymolysis liquid: crushing kudzu root bark by using a crusher, sieving the crushed kudzu root bark by using a 100-mesh sieve, adding 10 times of water by weight, uniformly mixing, pulping to obtain kudzu root bark slurry, pre-boiling the slurry at 85-100 ℃ for 30-60 min to fully gelatinize the slurry to obtain gelatinized liquid, adjusting the pH to 5.0-6.5, adding high-temperature amylase (40000U/g) at the concentration of 0.1-0.5 g/L, placing the gelatinized liquid in a 90-95 ℃ water bath kettle, performing enzymolysis for 2-3 h, cooling to 50 ℃, adjusting the pH to 4.5-5.0, adding complex enzyme (the complex enzyme is a mixture of 40000U/g amylase and 30000U/g cellulase) at the concentration of 0.04-0.08 g/L, placing the gelatinized liquid in a 45-55 ℃ water bath kettle, performing enzymolysis for 1-2 h, and deactivating enzyme to obtain kudzu root bark enzymatic hydrolysate;

(c3) preparing a kudzuvine root bark fermentation liquid: adding 2-5% of a nutritional aid (the nutritional aid is selected from glucose, fructose, xylose, mannose, galactose and erythrose) into the pueraria lobata skin enzymolysis liquid, sterilizing at 90-95 ℃ for 5min, and rapidly cooling to 35-40 ℃; and adding activated lactobacillus liquid (the volume ratio of the enzymolysis liquid to the lactobacillus liquid is 100: 3-5), carrying out anaerobic fermentation for 16-72 h at 35-40 ℃, immediately cooling to 2-10 ℃ when the pH of the fermentation liquid reaches 3.5-4.0, stopping fermentation, filtering with a 200-500-mesh sieve, and sterilizing the filtrate at 90-95 ℃ for 5min to obtain the pueraria lobata skin fermentation liquid.

9. The method of claim 7, wherein:

the dendrobium officinale leaf leaching liquor is prepared by the following method:

(a1) withering and rolling: spreading and airing the freshly picked dendrobium officinale leaves, placing the leaves in a dark and ventilated place for naturally withering for 8h, then airing to enable the fresh leaves to be in a wilted state, separating old and tender leaves in a rolling chamber with the temperature of 22 ℃ and the humidity of 85%, slightly pressing and short rolling tender leaves, rolling old leaves by a medium-pressure hairpin, uniformly rolling, and rolling the leaves into strips;

(a2) fermentation: putting the rolled dendrobium officinale leaves into a fermentation frame, putting the dendrobium officinale leaves into a fermentation chamber for fermentation, keeping the temperature of the fermented leaves at 28 ℃, and spreading the leaves with the thickness of 5 cm;

(a3) and (3) drying: drying the fermented leaves at 75 ℃ until the water content is 15%, spreading for cooling, continuously baking at a high temperature of 95 ℃ until the water content of the fermented leaves is 3-4%, and crushing into powder with the size of 0.5mm³Crushing the rice;

(a4) leaching: adding 10 times of water into the fermented leaf granules, leaching at 90 deg.C for 45min, rapidly cooling to 20 deg.C, and filtering with 200 mesh sieve to obtain herba Dendrobii leaf leaching solution;

the dendrobium officinale leaf fermentation liquor is prepared by the following method:

(b1) activating strains: mixing the powder (containing Lactobacillus paracasei 3.0 × 10)¹⁰cfu/g, Lactobacillus acidophilus 1.5X 10¹⁰cfu/g and Lactobacillus plantarum 2.0X 10¹⁰cfu/g) is added into 10 times of sterile distilled water with the temperature of 37 ℃ for dissolution, and then the mixture is placed in a thermostat with the temperature of 37 ℃ for activation for 30min to obtain lactic acid bacteria liquid for later use;

(b2) preparing the dendrobium officinale leaf enzymatic hydrolysate: adding freshly picked dendrobium officinale leaves into water with the weight 5 times that of the leaves to perform cell wall breaking treatment, so that the wall breaking rate reaches more than 60%, then pre-boiling for 20min at 90 ℃, then rapidly cooling to 50 ℃, adjusting the pH value to 4.5-5.0, adding a complex enzyme (containing 16000U/g pectinase and 30000U/g cellulase) at the concentration of 0.06g/L, placing the complex enzyme in a water bath kettle at 50 ℃, performing enzymolysis for 1.5h, and performing enzyme deactivation to obtain an enzymatic hydrolysate of the dendrobium officinale;

(b3) preparing a dendrobium officinale leaf fermentation liquid: adding 3% of nutritional adjuvants (selected from glucose, fructose, xylose, mannose, galactose and erythrose) into the enzymatic hydrolysate, sterilizing at 95 deg.C for 5min, and rapidly cooling to 37 deg.C; adding activated lactobacillus liquid (the volume ratio of the enzymolysis liquid to the lactobacillus liquid is 100: 2.5), carrying out anaerobic fermentation at 37 ℃ for 16-72 h, immediately cooling to 4 ℃ when the pH of the fermentation liquid reaches 3.5-4.0, stopping fermentation, filtering with a 200-mesh sieve, and sterilizing the filtrate at 95 ℃ for 5min to obtain dendrobium officinale leaf fermentation liquid; and/or

The kudzuvine root bark fermentation liquor is prepared by the following method:

(c1) activating strains: obtaining lactic acid bacteria liquid for later use according to the method in the step (b 1);

(c2) preparation of pueraria lobata skin enzymolysis liquid: crushing kudzuvine root bark by using a crusher, sieving the crushed kudzuvine root bark by using a 100-mesh sieve, adding 10 times of water by weight, uniformly mixing, pulping to obtain kudzuvine root bark pulp, pre-boiling the pulp at 90 ℃ for 45min to fully gelatinize the pulp to obtain gelatinized liquid, adjusting the pH to 5.0-5.5, adding high-temperature amylase (40000U/g) at the concentration of 0.25g/L, placing the gelatinized liquid in a 95 ℃ water bath kettle, performing enzymolysis for 2.5h, cooling to 50 ℃, adjusting the pH to 4.5-5.0, adding complex enzyme (the complex enzyme is a mixture of 40000U/g amylase and 30000U/g cellulase) at the concentration of 0.05g/L, placing the complex enzyme in a 50 ℃ water bath kettle, performing enzymolysis for 1.5h, and deactivating the enzyme to obtain kudzuvine root bark enzymatic hydrolysate;

(c3) preparing a kudzuvine root bark fermentation liquid: adding 3% of nutritional adjuvants (selected from glucose, fructose, xylose, mannose, galactose and erythrose) into the enzymatic hydrolysate of radix Puerariae peel, sterilizing at 95 deg.C for 5min, and rapidly cooling to 37 deg.C; and adding activated lactobacillus liquid (the volume ratio of the enzymolysis liquid to the lactobacillus liquid is 100: 3.5), carrying out anaerobic fermentation at 37 ℃ for 16-72 h, immediately cooling to 4 ℃ when the pH of the fermentation liquid reaches 3.5-4.0, stopping fermentation, filtering with a 200-mesh sieve, and sterilizing the filtrate at 95 ℃ for 5min to obtain the pueraria lobata skin fermentation liquid.

10. The method of claim 7, wherein:

when the composition is prepared, the dendrobium officinale leaf leaching liquor, the dendrobium officinale leaf fermentation liquor and the kudzuvine root bark fermentation liquor are uniformly mixed, the corn oligopeptide powder is added to dissolve, the flavoring agent is optionally added to dissolve, and/or the optional water is added to dilute, so that the composition is obtained; and/or

It also comprises the operations of filling and sterilizing the obtained composition; for example, the sterilization is UHT sterilization; for example, the UHT sterilization is sterilization at 135-140 ℃ for 4-10 seconds.

Technical Field

The invention belongs to the technical field of medicines, relates to a composition for relieving alcoholism, and particularly relates to an anti-alcoholism composition prepared from dendrobium officinale, radix puerariae and corn peptide. In addition, the composition of the invention also has excellent liver protection effect

Background

Dendrobium officinale is a rare and rare medicinal plant in Orchidaceae, is the top grade of Dendrobium nobile, is the head of the "Jiuda Mesona chinensis Benth", and has high medicinal value. Although the medicinal part of the dendrobium officinale is the stem, the research on flowers and leaves is less, and the research on literature data shows that the dendrobium officinale stem and leaves have the same functional components, and the polysaccharide content of the leaves is about one third of that of the stem. Modern medicine shows that the dendrobium officinale leaf mainly contains polysaccharide as an effective component and has the effects of enhancing immunity, resisting tumors, reducing blood fat, resisting oxidation and the like.

Radix Puerariae is root of Pueraria lobata Ohwi of Leguminosae or dried root of Pueraria thomsonii, and is mostly grown in wild flora and deep soil. In ancient times, Kudzuvine root was listed as "guan-drug". The kudzu root is a plant which is approved by the Ministry of health of China and can be used as both medicine and food, is called Asian ginseng, and has a magical effect of containing about 12 percent of flavonoids. These compounds mainly comprise more than ten effective components such as soybean (glycitin), daidzein, puerarin, etc. Kudzu root is sweet and pungent in taste, neutral in nature, and enters spleen and stomach meridians, the earliest medical monograph ' Shennong herbal Jing ' in China has detailed records, the famous prescription ' kudzu root decoction ' listed in the ' Shang Han Lun ' of Sheng Zhang Zhongjing in the Han Dynasty is still widely used as an important exterior-resolving prescription so far, the Tang Dynasty Ben ' describes the efficacy of kudzu root on relieving alcoholism in the ' dietetic herbal ', the kudzu root is still regarded as a sobering-up ' Shengpin ', and the modern medicine proves that the kudzu root has the function of expanding blood vessels and coronary arteries, can effectively increase the blood flow of brain and coronary arteries, and has unique curative effects on angina caused by hypertension, cardiovascular sclerosis, arrhythmia, coronary heart disease and the like.

The corn oligopeptide (corn peptide) has reliable safety. The corn peptide has various biological activities, such as antioxidation, hypertension resistance, immunity enhancement, fatigue resistance, liver protection and the like. It is noted that animal and human experiments have found that corn peptide also has an important but different functional property from other food-derived oligopeptides, namely, sobering-up effect, thereby protecting the liver.

Disclosure of Invention

The invention aims to provide an anti-inebriation composition with a simple formula to realize the anti-inebriation effect. The present inventors have surprisingly found that a composition prepared from corn oligopeptide powder, dendrobium officinale and pueraria lobata as raw materials exhibits excellent technical effects by using the method of the present invention, and the present invention has been completed based on this finding.

Therefore, in the first aspect of the invention, the dendrobium officinale alcohol-effect-dispelling and liver-protecting composition is prepared from dendrobium officinale leaves, pueraria lobata peels and corn oligopeptide.

According to the first aspect of the invention, the dendrobium officinale alcohol-effect-relieving and liver-protecting composition is added into the alcohol-effect-relieving composition in the form of a dendrobium officinale leaf leaching liquor and a dendrobium officinale leaf fermentation liquor.

According to the dendrobium officinale alcohol effect relieving and liver protecting composition of the first aspect of the invention, the pueraria lobata peel is added into the alcohol effect relieving composition in the form of pueraria lobata peel fermentation liquor.

According to the first aspect of the invention, the dendrobium officinale alcohol-effect-dispelling and liver-protecting composition is prepared by the following method:

(a1) withering and rolling: spreading and airing the freshly picked dendrobium officinale leaves, placing the leaves in a dark and ventilated place for naturally withering for 8-10 h, then airing to enable the fresh leaves to be in a wilted state, separating old and tender leaves in a rolling chamber with the temperature of 20-24 ℃ and the humidity of 85-90%, slightly pressing and short rolling the tender leaves, rolling the old leaves by a medium-pressure hairpin, uniformly rolling, and rolling the leaves into strips;

(a2) fermentation: putting the rolled dendrobium officinale leaves into a fermentation frame, putting the dendrobium officinale leaves into a fermentation chamber for fermentation, keeping the temperature of the fermented leaves at 27-30 ℃, and spreading the leaves to be 5-15 cm thick;

(a3) and (3) drying: drying the fermented leaves at 70-80 ℃ until the water content is 15-20%, spreading for cooling, continuously baking at the high temperature of 95-105 ℃ until the water content of the fermented leaves is 3-8%, and crushing into powder with the size of 0.5mm³Crushing the rice;

(a4) leaching: adding 10-50 times of water by weight into the obtained fermented leaf particles, leaching for 30-60 min at 85-100 ℃, then rapidly cooling to 20 ℃, and filtering through a 200-500-mesh sieve to obtain the dendrobium officinale leaf leaching liquor.

According to the first aspect of the invention, the dendrobium officinale alcohol-effect-dispelling and liver-protecting composition is prepared by the following method:

(b1) activating strains: mixing the powder (containing Lactobacillus paracasei 3.0 × 10)¹⁰cfu/g, Lactobacillus acidophilus 1.5X 10¹⁰cfu/g and Lactobacillus plantarum 2.0X 10¹⁰cfu/g) is added into 10-20 times of sterile distilled water with the temperature of 35-40 ℃ for dissolving, and then the mixture is placed in a thermostat with the temperature of 37-39 ℃ for activation for 30min to obtain lactic acid bacteria liquid for later use;

(b2) preparing the dendrobium officinale leaf enzymatic hydrolysate: adding freshly picked dendrobium officinale leaves into water with the weight 5 times that of the leaves to perform cell wall breaking treatment, so that the wall breaking rate reaches more than 60%, then pre-boiling the leaves at 85-95 ℃ for 10-30 min, then rapidly cooling the leaves to 45-55 ℃, adjusting the pH value to 4.5-5.0, adding a complex enzyme (containing 16000U/g pectinase and 30000U/g cellulase) at the concentration of 0.04-0.08 g/L, placing the complex enzyme in a water bath kettle at 45-55 ℃, performing enzymolysis for 1-2 h, and inactivating the enzyme to obtain an enzymolysis solution of the dendrobium officinale;

(b3) preparing a dendrobium officinale leaf fermentation liquid: adding 2-5% of a nutritional aid (the nutritional aid is selected from glucose, fructose, xylose, mannose, galactose and erythrose) into the dendrobium officinale leaf enzymatic hydrolysate, sterilizing at 90-95 ℃ for 5min, and rapidly cooling to 35-40 ℃; and adding activated lactobacillus liquid (the volume ratio of the enzymolysis liquid to the lactobacillus liquid is 100: 2-3), carrying out anaerobic fermentation at 35-40 ℃ for 16-72 h, immediately cooling to 2-10 ℃ when the pH of the fermentation liquid reaches 3.5-4.0, stopping fermentation, filtering with a 200-500-mesh sieve, and sterilizing the filtrate at 90-95 ℃ for 5min to obtain the dendrobium officinale leaf fermentation liquid.

According to the first aspect of the invention, the dendrobium officinale alcohol-effect-dispelling and liver-protecting composition is prepared by the following method:

(c1) activating strains: obtaining lactic acid bacteria liquid for later use according to the method in the step (b 1);

(c2) preparation of pueraria lobata skin enzymolysis liquid: crushing kudzu root bark by using a crusher, sieving the crushed kudzu root bark by using a 100-mesh sieve, adding 10 times of water by weight, uniformly mixing, pulping to obtain kudzu root bark slurry, pre-boiling the slurry at 85-100 ℃ for 30-60 min to fully gelatinize the slurry to obtain gelatinized liquid, adjusting the pH to 5.0-6.5, adding high-temperature amylase (40000U/g) at the concentration of 0.1-0.5 g/L, placing the gelatinized liquid in a 90-95 ℃ water bath kettle, performing enzymolysis for 2-3 h, cooling to 50 ℃, adjusting the pH to 4.5-5.0, adding complex enzyme (the complex enzyme is a mixture of 40000U/g amylase and 30000U/g cellulase) at the concentration of 0.04-0.08 g/L, placing the gelatinized liquid in a 45-55 ℃ water bath kettle, performing enzymolysis for 1-2 h, and deactivating enzyme to obtain kudzu root bark enzymatic hydrolysate;

(c3) preparing a kudzuvine root bark fermentation liquid: adding 2-5% of a nutritional aid (the nutritional aid is selected from glucose, fructose, xylose, mannose, galactose and erythrose) into the pueraria lobata skin enzymolysis liquid, sterilizing at 90-95 ℃ for 5min, and rapidly cooling to 35-40 ℃; and adding activated lactobacillus liquid (the volume ratio of the enzymolysis liquid to the lactobacillus liquid is 100: 3-5), carrying out anaerobic fermentation for 16-72 h at 35-40 ℃, immediately cooling to 2-10 ℃ when the pH of the fermentation liquid reaches 3.5-4.0, stopping fermentation, filtering with a 200-500-mesh sieve, and sterilizing the filtrate at 90-95 ℃ for 5min to obtain the pueraria lobata skin fermentation liquid.

According to the first aspect of the invention, the dendrobium officinale alcohol-effect-dispelling and liver-protecting composition is prepared by the following method:

(a1) withering and rolling: spreading and airing the freshly picked dendrobium officinale leaves, placing the leaves in a dark and ventilated place for naturally withering for 8h, then airing to enable the fresh leaves to be in a wilted state, separating old and tender leaves in a rolling chamber with the temperature of 22 ℃ and the humidity of 85%, slightly pressing and short rolling tender leaves, rolling old leaves by a medium-pressure hairpin, uniformly rolling, and rolling the leaves into strips;

(a2) fermentation: putting the rolled dendrobium officinale leaves into a fermentation frame, putting the dendrobium officinale leaves into a fermentation chamber for fermentation, keeping the temperature of the fermented leaves at 28 ℃, and spreading the leaves with the thickness of 5 cm;

(a3) and (3) drying: drying the fermented leaves at 75 ℃ until the water content is 15%, spreading for cooling, continuously baking at a high temperature of 95 ℃ until the water content of the fermented leaves is 3-4%, and crushing into powder with the size of 0.5mm³Crushing the rice;

(a4) leaching: adding 10 times of water into the obtained fermented leaf granules, leaching at 90 deg.C for 45min, rapidly cooling to 20 deg.C, and filtering with 200 mesh sieve to obtain herba Dendrobii leaf leaching solution.

According to the first aspect of the invention, the dendrobium officinale alcohol-effect-dispelling and liver-protecting composition is prepared by the following method:

(b1) activating strains: mixing the powder (containing Lactobacillus paracasei 3.0 × 10)¹⁰cfu/g, Lactobacillus acidophilus 1.5X 10¹⁰cfu/g and Lactobacillus plantarum 2.0X 10¹⁰cfu/g) is added into 10 times of sterile distilled water with the temperature of 37 ℃ for dissolution, and then the mixture is placed in a thermostat with the temperature of 37 ℃ for activation for 30min to obtain lactic acid bacteria liquid for later use;

(b2) preparing the dendrobium officinale leaf enzymatic hydrolysate: adding freshly picked dendrobium officinale leaves into water with the weight 5 times that of the leaves to perform cell wall breaking treatment, so that the wall breaking rate reaches more than 60%, then pre-boiling for 20min at 90 ℃, then rapidly cooling to 50 ℃, adjusting the pH value to 4.5-5.0, adding a complex enzyme (containing 16000U/g pectinase and 30000U/g cellulase) at the concentration of 0.06g/L, placing the complex enzyme in a water bath kettle at 50 ℃, performing enzymolysis for 1.5h, and performing enzyme deactivation to obtain an enzymatic hydrolysate of the dendrobium officinale;

(b3) preparing a dendrobium officinale leaf fermentation liquid: adding 3% of nutritional adjuvants (selected from glucose, fructose, xylose, mannose, galactose and erythrose) into the enzymatic hydrolysate, sterilizing at 95 deg.C for 5min, and rapidly cooling to 37 deg.C; and adding activated lactobacillus liquid (the volume ratio of the enzymolysis liquid to the lactobacillus liquid is 100: 2.5), carrying out anaerobic fermentation at 37 ℃ for 16-72 h, immediately cooling to 4 ℃ when the pH of the fermentation liquid reaches 3.5-4.0, stopping fermentation, filtering with a 200-mesh sieve, and sterilizing the filtrate at 95 ℃ for 5min to obtain the dendrobium officinale leaf fermentation liquid.

According to the first aspect of the invention, the dendrobium officinale alcohol-effect-dispelling and liver-protecting composition is prepared by the following method:

(c1) activating strains: obtaining lactic acid bacteria liquid for later use according to the method in the step (b 1);

(c2) preparation of pueraria lobata skin enzymolysis liquid: crushing kudzuvine root bark by using a crusher, sieving the crushed kudzuvine root bark by using a 100-mesh sieve, adding 10 times of water by weight, uniformly mixing, pulping to obtain kudzuvine root bark pulp, pre-boiling the pulp at 90 ℃ for 45min to fully gelatinize the pulp to obtain gelatinized liquid, adjusting the pH to 5.0-5.5, adding high-temperature amylase (40000U/g) at the concentration of 0.25g/L, placing the gelatinized liquid in a 95 ℃ water bath kettle, performing enzymolysis for 2.5h, cooling to 50 ℃, adjusting the pH to 4.5-5.0, adding complex enzyme (the complex enzyme is a mixture of 40000U/g amylase and 30000U/g cellulase) at the concentration of 0.05g/L, placing the complex enzyme in a 50 ℃ water bath kettle, performing enzymolysis for 1.5h, and deactivating the enzyme to obtain kudzuvine root bark enzymatic hydrolysate;

(c3) preparing a kudzuvine root bark fermentation liquid: adding 3% of nutritional adjuvants (selected from glucose, fructose, xylose, mannose, galactose and erythrose) into the enzymatic hydrolysate of radix Puerariae peel, sterilizing at 95 deg.C for 5min, and rapidly cooling to 37 deg.C; and adding activated lactobacillus liquid (the volume ratio of the enzymolysis liquid to the lactobacillus liquid is 100: 3.5), carrying out anaerobic fermentation at 37 ℃ for 16-72 h, immediately cooling to 4 ℃ when the pH of the fermentation liquid reaches 3.5-4.0, stopping fermentation, filtering with a 200-mesh sieve, and sterilizing the filtrate at 95 ℃ for 5min to obtain the pueraria lobata skin fermentation liquid.

The dendrobium officinale alcohol-effect-dispelling and liver-protecting composition comprises the following components in parts by weight:

10-20 parts of dendrobium officinale leaf leaching liquor,

5-10 parts of dendrobium officinale leaf fermentation liquor,

10-30 parts of kudzu root bark fermentation liquor,

0.2-0.6 part of corn oligopeptide powder.

The dendrobium officinale alcohol-effect-dispelling and liver-protecting composition comprises the following components in parts by weight:

15 parts of dendrobium officinale leaf leaching liquor,

8 parts of dendrobium officinale leaf fermentation liquor,

20 portions of pueraria root bark fermentation liquor,

0.4 part of corn oligopeptide powder.

The dendrobium officinale alcohol-effect-relieving and liver-protecting composition according to the first aspect of the invention can be diluted with water. In one embodiment, the dendrobium officinale alcohol-effect-dispelling and liver-protecting composition comprises:

10-20% of dendrobium officinale leaf leaching liquor,

5-10% of dendrobium officinale leaf fermentation liquor,

10-30% of pueraria peel fermentation liquor,

0.2-0.6% of corn oligopeptide powder,

Adding water to 100%.

The dendrobium officinale alcohol-effect-relieving and liver-protecting composition according to the first aspect of the invention can be diluted with water. In one embodiment, the dendrobium officinale alcohol-effect-dispelling and liver-protecting composition comprises:

15 percent of dendrobium officinale leaf leaching liquor,

8 percent of dendrobium officinale leaf fermentation liquor,

20 percent of kudzuvine root bark fermentation liquor,

0.4 percent of corn oligopeptide powder,

Adding water to 100%.

The dendrobium officinale alcohol-effect-relieving and liver-protecting composition according to the first aspect of the invention may further comprise a flavoring agent, such as sucrose, crystal sugar, lemon juice such as lemon juice and the like, and when the flavoring agent is added, the flavoring agent is generally added in an amount which is conventional for the purpose of endowing the composition with a suitable taste, such as 2-10% such as 5% of sucrose, crystal sugar and the like, and 1-2% such as 1.5% of lemon juice.

Further, the invention provides a method for preparing the dendrobium officinale alcohol-effect-dispelling and liver-protecting composition in the second aspect, which comprises the following steps:

(1) providing a leaching solution of dendrobium officinale leaves;

(2) providing a dendrobium officinale leaf fermentation liquid;

(3) providing pueraria lobata peel fermentation liquor;

(4) and (4) preparing the composition.

According to the second aspect of the invention, the leaching solution of the dendrobium officinale leaves is prepared according to the following method:

(a1) withering and rolling: spreading and airing the freshly picked dendrobium officinale leaves, placing the leaves in a dark and ventilated place for naturally withering for 8-10 h, then airing to enable the fresh leaves to be in a wilted state, separating old and tender leaves in a rolling chamber with the temperature of 20-24 ℃ and the humidity of 85-90%, slightly pressing and short rolling the tender leaves, rolling the old leaves by a medium-pressure hairpin, uniformly rolling, and rolling the leaves into strips;

(a2) fermentation: putting the rolled dendrobium officinale leaves into a fermentation frame, putting the dendrobium officinale leaves into a fermentation chamber for fermentation, keeping the temperature of the fermented leaves at 27-30 ℃, and spreading the leaves to be 5-15 cm thick;

(a3) and (3) drying: drying the fermented leaves at 70-80 ℃ until the water content is 15-20%, spreading for cooling, continuously baking at the high temperature of 95-105 ℃ until the water content of the fermented leaves is 3-8%, and crushing into powder with the size of 0.5mm³Crushing the rice;

(a4) leaching: adding 10-50 times of water by weight into the obtained fermented leaf particles, leaching for 30-60 min at 85-100 ℃, then rapidly cooling to 20 ℃, and filtering through a 200-500-mesh sieve to obtain the dendrobium officinale leaf leaching liquor.

According to the method of the second aspect of the invention, the fermentation liquid of dendrobium officinale leaves is prepared according to the following method:

(b1) activating strains: mixing the powder (containing Lactobacillus paracasei 3.0 × 10)¹⁰cfu/g, Lactobacillus acidophilus 1.5X 10¹⁰cfu/g and Lactobacillus plantarum 2.0X 10¹⁰cfu/g) is added into 10-20 times of sterile distilled water with the temperature of 35-40 ℃ for dissolving, and then the mixture is placed in a thermostat with the temperature of 37-39 ℃ for activation for 30min to obtain lactic acid bacteria liquid for later use;

(b2) preparing the dendrobium officinale leaf enzymatic hydrolysate: adding freshly picked dendrobium officinale leaves into water with the weight 5 times that of the leaves to perform cell wall breaking treatment, so that the wall breaking rate reaches more than 60%, then pre-boiling the leaves at 85-95 ℃ for 10-30 min, then rapidly cooling the leaves to 45-55 ℃, adjusting the pH value to 4.5-5.0, adding a complex enzyme (containing 16000U/g pectinase and 30000U/g cellulase) at the concentration of 0.04-0.08 g/L, placing the complex enzyme in a water bath kettle at 45-55 ℃, performing enzymolysis for 1-2 h, and inactivating the enzyme to obtain an enzymolysis solution of the dendrobium officinale;

(b3) preparing a dendrobium officinale leaf fermentation liquid: adding 2-5% of a nutritional aid (the nutritional aid is selected from glucose, fructose, xylose, mannose, galactose and erythrose) into the dendrobium officinale leaf enzymatic hydrolysate, sterilizing at 90-95 ℃ for 5min, and rapidly cooling to 35-40 ℃; and adding activated lactobacillus liquid (the volume ratio of the enzymolysis liquid to the lactobacillus liquid is 100: 2-3), carrying out anaerobic fermentation at 35-40 ℃ for 16-72 h, immediately cooling to 2-10 ℃ when the pH of the fermentation liquid reaches 3.5-4.0, stopping fermentation, filtering with a 200-500-mesh sieve, and sterilizing the filtrate at 90-95 ℃ for 5min to obtain the dendrobium officinale leaf fermentation liquid.

According to the method of the second aspect of the present invention, the pueraria lobata skin fermentation liquid is prepared as follows:

(c1) activating strains: obtaining lactic acid bacteria liquid for later use according to the method in the step (b 1);

(c2) preparation of pueraria lobata skin enzymolysis liquid: crushing kudzu root bark by using a crusher, sieving the crushed kudzu root bark by using a 100-mesh sieve, adding 10 times of water by weight, uniformly mixing, pulping to obtain kudzu root bark slurry, pre-boiling the slurry at 85-100 ℃ for 30-60 min to fully gelatinize the slurry to obtain gelatinized liquid, adjusting the pH to 5.0-6.5, adding high-temperature amylase (40000U/g) at the concentration of 0.1-0.5 g/L, placing the gelatinized liquid in a 90-95 ℃ water bath kettle, performing enzymolysis for 2-3 h, cooling to 50 ℃, adjusting the pH to 4.5-5.0, adding complex enzyme (the complex enzyme is a mixture of 40000U/g amylase and 30000U/g cellulase) at the concentration of 0.04-0.08 g/L, placing the gelatinized liquid in a 45-55 ℃ water bath kettle, performing enzymolysis for 1-2 h, and deactivating enzyme to obtain kudzu root bark enzymatic hydrolysate;

(c3) preparing a kudzuvine root bark fermentation liquid: adding 2-5% of a nutritional aid (the nutritional aid is selected from glucose, fructose, xylose, mannose, galactose and erythrose) into the pueraria lobata skin enzymolysis liquid, sterilizing at 90-95 ℃ for 5min, and rapidly cooling to 35-40 ℃; and adding activated lactobacillus liquid (the volume ratio of the enzymolysis liquid to the lactobacillus liquid is 100: 3-5), carrying out anaerobic fermentation for 16-72 h at 35-40 ℃, immediately cooling to 2-10 ℃ when the pH of the fermentation liquid reaches 3.5-4.0, stopping fermentation, filtering with a 200-500-mesh sieve, and sterilizing the filtrate at 90-95 ℃ for 5min to obtain the pueraria lobata skin fermentation liquid.

According to the second aspect of the invention, the leaching solution of the dendrobium officinale leaves is prepared according to the following method:

(a1) withering and rolling: spreading and airing the freshly picked dendrobium officinale leaves, placing the leaves in a dark and ventilated place for naturally withering for 8h, then airing to enable the fresh leaves to be in a wilted state, separating old and tender leaves in a rolling chamber with the temperature of 22 ℃ and the humidity of 85%, slightly pressing and short rolling tender leaves, rolling old leaves by a medium-pressure hairpin, uniformly rolling, and rolling the leaves into strips;

(a2) fermentation: putting the rolled dendrobium officinale leaves into a fermentation frame, putting the dendrobium officinale leaves into a fermentation chamber for fermentation, keeping the temperature of the fermented leaves at 28 ℃, and spreading the leaves with the thickness of 5 cm;

(a3) and (3) drying: drying the fermented leaves at 75 ℃ until the water content is 15%, spreading for cooling, continuously baking at a high temperature of 95 ℃ until the water content of the fermented leaves is 3-4%, and crushing into powder with the size of 0.5mm³Crushing the rice;

(a4) leaching: adding 10 times of water into the obtained fermented leaf granules, leaching at 90 deg.C for 45min, rapidly cooling to 20 deg.C, and filtering with 200 mesh sieve to obtain herba Dendrobii leaf leaching solution.

According to the method of the second aspect of the invention, the fermentation liquid of dendrobium officinale leaves is prepared according to the following method:

(b1) activating strains: mixing the powder (containing Lactobacillus paracasei 3.0 × 10)¹⁰cfu/g, Lactobacillus acidophilus 1.5X 10¹⁰cfu/g and Lactobacillus plantarum 2.0X 10¹⁰cfu/g) is added into 10 times of sterile distilled water with the temperature of 37 ℃ for dissolution, and then the mixture is placed in a thermostat with the temperature of 37 ℃ for activation for 30min to obtain lactic acid bacteria liquid for later use;

(b2) preparing the dendrobium officinale leaf enzymatic hydrolysate: adding freshly picked dendrobium officinale leaves into water with the weight 5 times that of the leaves to perform cell wall breaking treatment, so that the wall breaking rate reaches more than 60%, then pre-boiling for 20min at 90 ℃, then rapidly cooling to 50 ℃, adjusting the pH value to 4.5-5.0, adding a complex enzyme (containing 16000U/g pectinase and 30000U/g cellulase) at the concentration of 0.06g/L, placing the complex enzyme in a water bath kettle at 50 ℃, performing enzymolysis for 1.5h, and performing enzyme deactivation to obtain an enzymatic hydrolysate of the dendrobium officinale;

(b3) preparing a dendrobium officinale leaf fermentation liquid: adding 3% of nutritional adjuvants (selected from glucose, fructose, xylose, mannose, galactose and erythrose) into the enzymatic hydrolysate, sterilizing at 95 deg.C for 5min, and rapidly cooling to 37 deg.C; and adding activated lactobacillus liquid (the volume ratio of the enzymolysis liquid to the lactobacillus liquid is 100: 2.5), carrying out anaerobic fermentation at 37 ℃ for 16-72 h, immediately cooling to 4 ℃ when the pH of the fermentation liquid reaches 3.5-4.0, stopping fermentation, filtering with a 200-mesh sieve, and sterilizing the filtrate at 95 ℃ for 5min to obtain the dendrobium officinale leaf fermentation liquid.

According to the method of the second aspect of the present invention, the pueraria lobata skin fermentation liquid is prepared as follows:

(c1) activating strains: obtaining lactic acid bacteria liquid for later use according to the method in the step (b 1);

(c2) preparation of pueraria lobata skin enzymolysis liquid: crushing kudzuvine root bark by using a crusher, sieving the crushed kudzuvine root bark by using a 100-mesh sieve, adding 10 times of water by weight, uniformly mixing, pulping to obtain kudzuvine root bark pulp, pre-boiling the pulp at 90 ℃ for 45min to fully gelatinize the pulp to obtain gelatinized liquid, adjusting the pH to 5.0-5.5, adding high-temperature amylase (40000U/g) at the concentration of 0.25g/L, placing the gelatinized liquid in a 95 ℃ water bath kettle, performing enzymolysis for 2.5h, cooling to 50 ℃, adjusting the pH to 4.5-5.0, adding complex enzyme (the complex enzyme is a mixture of 40000U/g amylase and 30000U/g cellulase) at the concentration of 0.05g/L, placing the complex enzyme in a 50 ℃ water bath kettle, performing enzymolysis for 1.5h, and deactivating the enzyme to obtain kudzuvine root bark enzymatic hydrolysate;

(c3) preparing a kudzuvine root bark fermentation liquid: adding 3% of nutritional adjuvants (selected from glucose, fructose, xylose, mannose, galactose and erythrose) into the enzymatic hydrolysate of radix Puerariae peel, sterilizing at 95 deg.C for 5min, and rapidly cooling to 37 deg.C; and adding activated lactobacillus liquid (the volume ratio of the enzymolysis liquid to the lactobacillus liquid is 100: 3.5), carrying out anaerobic fermentation at 37 ℃ for 16-72 h, immediately cooling to 4 ℃ when the pH of the fermentation liquid reaches 3.5-4.0, stopping fermentation, filtering with a 200-mesh sieve, and sterilizing the filtrate at 95 ℃ for 5min to obtain the pueraria lobata skin fermentation liquid.

The method according to the second aspect of the present invention, wherein the composition comprises the following components in the following weight ratio:

10-20 parts of dendrobium officinale leaf leaching liquor,

5-10 parts of dendrobium officinale leaf fermentation liquor,

10-30 parts of kudzu root bark fermentation liquor,

0.2-0.6 part of corn oligopeptide powder.

The method according to the second aspect of the present invention, wherein the composition comprises the following components in the following weight ratio:

15 parts of dendrobium officinale leaf leaching liquor,

8 parts of dendrobium officinale leaf fermentation liquor,

20 portions of pueraria root bark fermentation liquor,

0.4 part of corn oligopeptide powder.

The method according to the second aspect of the present invention, wherein the composition may further be diluted with water. In one embodiment, the dendrobium officinale alcohol-effect-dispelling and liver-protecting composition comprises:

10-20% of dendrobium officinale leaf leaching liquor,

5-10% of dendrobium officinale leaf fermentation liquor,

10-30% of pueraria peel fermentation liquor,

0.2-0.6% of corn oligopeptide powder.

Adding water to 100%.

The method according to the second aspect of the present invention, wherein the composition may further be diluted with water. In one embodiment, the dendrobium officinale alcohol-effect-dispelling and liver-protecting composition comprises:

15 percent of dendrobium officinale leaf leaching liquor,

8 percent of dendrobium officinale leaf fermentation liquor,

20 percent of kudzuvine root bark fermentation liquor,

0.4 percent of corn oligopeptide powder,

Adding water to 100%.

The method according to the second aspect of the invention wherein the composition may also comprise flavouring agents such as sucrose, ice candy, lemon juice such as lemon juice and the like, which flavouring agents, when added, are typically added in amounts conventionally used to give the composition a suitable mouthfeel, for example sucrose, ice candy and the like may be added in amounts of 2 to 10%, for example 5%, and for example lemon juice may be added in amounts of 1 to 2%, for example 1.5%.

According to the method of the second aspect of the present invention, the composition is prepared by mixing the leaching solution of the dendrobium officinale leaf, the fermentation solution of the dendrobium officinale leaf and the fermentation solution of the pueraria lobata skin uniformly, adding the corn oligopeptide powder to dissolve, optionally adding the flavoring agent to dissolve, and/or optionally adding water to dilute, to obtain the composition.

The method according to the second aspect of the present invention, further comprising the operation of filling and sterilizing the resulting composition. In one embodiment, the sterilization is UHT sterilization (i.e., ultra high temperature flash sterilization). In one embodiment, the UHT sterilization is sterilization at 135-140 ℃ for 4-10 seconds.

The inventor has found that the dendrobium officinale leaf leaching liquor and the dendrobium officinale leaf fermentation liquor are combined with the kudzuvine root bark fermentation liquor and the corn oligopeptide powder in a specific ratio to have an unexpected anti-alcohol effect.

In the above-described steps of the preparation method of the present invention, although the specific steps described therein are distinguished in some detail or in language description from the steps described in the preparation examples of the detailed embodiments below, those skilled in the art can fully summarize the above-described method steps in light of the detailed disclosure throughout the present disclosure.

Any embodiment of any aspect of the invention may be combined with other embodiments, as long as they do not contradict. Furthermore, in any embodiment of any aspect of the invention, any feature may be applicable to that feature in other embodiments, so long as they do not contradict.

The invention is further described below.

In the present invention, the corn oligopeptide is readily available from commercial sources, such as from Chongqing Biopsis.

At present, a plurality of antialcoholic beverages are available on the market, the antialcoholic effect is not good, and the pueraria is mainly matched with other Chinese medicinal materials to be simply extracted, and then the other raw materials are matched to be simply blended. The active ingredients of the product are improved in process design and formula composition, and the functional anti-alcohol beverage is pure in taste and clean in label. The invention utilizes the specific medicinal values of dendrobium officinale leaf, kudzu root and corn oligopeptide powder to prepare the anti-alcohol beverage which has unique taste and strong anti-alcohol effect, has the functions of promoting liver detoxification, kidney excretion, recovering damaged gastrointestinal mucosa and rebuilding intestinal flora, and has the effects of sobering up, protecting liver and promoting recovery of intestines and stomach after drinking.

The beverage disclosed by the invention utilizes the stomach nourishing effect of the dendrobium officinale leaf, combines the functions of dispelling the effects of alcohol and protecting the liver of the food material kudzu root and the corn oligopeptide which is a new food material with homology of medicine and food, overcomes the defect that the gastrointestinal recovery effect of the traditional alcohol-dispelling beverage after drinking is not strong, has the effects of dispelling the effects of alcohol, protecting the liver and nourishing the stomach, and can be drunk as an alcohol partner.

The invention provides an anti-inebriation beverage and a preparation method thereof, and the method comprises two aspects: firstly, preparing a leaching solution of dendrobium officinale leaf tea by using raw materials, rolling, naturally fermenting, leaching and the like; secondly, preparing a dendrobium officinale leaf fermentation liquid, wherein the main processes comprise raw material pretreatment, cell wall breaking, biological enzymolysis, microbial fermentation and the like; thirdly, preparing the pueraria lobata peel fermentation liquor by the main processes of cell wall breaking, biological enzymolysis, microbial fermentation and the like. The process can improve the utilization rate of active ingredients of the dendrobium officinale leaves and the kudzuvine roots, remove bitter and astringent taste and green taste, improve the taste and color, and simultaneously ensure that functional ingredients are more beneficial to the absorption of a human body, thereby achieving the effects of dispelling the effects of alcohol and protecting the liver.

The technical scheme adopted by the invention is as follows: processing fresh dendrobium officinale leaves by adopting a fermentation technology for preparing black tea to prepare dendrobium officinale leaf black tea, and preparing a dendrobium officinale leaf black tea leaching liquor by processes such as leaching, filtering and the like; performing cell wall breaking and biological enzymolysis treatment on fresh dendrobium officinale leaves to prepare an enzymolysis solution of the dendrobium officinale leaves, and performing probiotic fermentation, filtration and sterilization to prepare a fermentation solution of the dendrobium officinale leaves; treating the kudzu vine root bark by an enzymolysis process to prepare kudzu vine root bark enzymolysis liquid, and preparing the kudzu vine root bark fermentation liquid by probiotic fermentation, filtration and sterilization.

The dendrobium officinale leaf anti-alcoholic beverage is recorded as follows by mass: 0-30% of dendrobium officinale leaching liquor, 0-30% of dendrobium officinale fermentation liquor, 0-30% of kudzu root bark fermentation liquor, 0-1% of corn oligopeptide powder, and the balance of other flavor ingredients (added or not added) and water.

The dendrobium officinale anti-alcoholic beverage disclosed by the invention has excellent technical effects.

Detailed Description

The present invention will be further described by the following examples, however, the scope of the present invention is not limited to the following examples. It will be understood by those skilled in the art that various changes and modifications may be made to the invention without departing from the spirit and scope of the invention. The present invention has been described generally and/or specifically with respect to materials used in testing and testing methods. Although many materials and methods of operation are known in the art for the purpose of carrying out the invention, the invention is nevertheless described herein in as detail as possible. The following examples further illustrate the invention without limiting it.

The following preparation steps are given for the purpose of illustration and are based on the comparative nature of the respective examples and the person skilled in the art is fully enabled to generalize from the prior knowledge the process of the invention for preparing the products of the invention.

In the following examples of preparing the dendrobium officinale anti-alcoholic beverage of the present invention, the processing amount of each lot is not less than 20kg, if not otherwise specified.