阅读说明：本技术 一种可促进牛蛙生长发育的发酵型饲料及制备方法 (Fermented feed capable of promoting growth and development of bullfrogs and preparation method thereof ) 是由 张浩然 江谢武 林天彪 尹建雄 许海辉 张涛 安振强 于 2019-11-29 设计创作，主要内容包括：本发明公开了一种可促进牛蛙生长发育的发酵型饲料,以重量份数计,包括鱼粉15-30份、肉骨粉5-10份、鸡肉粉10-20份、花生粕17-30份、玉米蛋白粉5-12份、面粉16-30份、蛙用维生素预混合饲料0.5-1份、蛙用多矿预混合饲料0.5-1份、胆汁酸0.4-0.8份,以重量计,还包括发酵组分：酵母菌1-3Kg、乳酸复合菌1-2Kg、芽孢复合菌1-2.5Kg、复合酶0.5-1Kg。还公开了一种所述可促进牛蛙生长发育的发酵型饲料的制备方法。本发明原材料获取简便,制造方法简单可行,可有效解决高密度养殖模式中牛蛙生长速率慢、消化率低导致的肠道负担重、肠道不健康、抗病性弱等问题,在养殖期间,可以显著提高牛蛙的特定增长率,降低饲料系数,减少用药量,较大幅度地提高养殖效益。(The invention discloses a fermented feed capable of promoting the growth and development of bullfrogs, which comprises 15-30 parts of fish meal, 5-10 parts of meat and bone meal, 10-20 parts of chicken meal, 17-30 parts of peanut meal, 5-12 parts of corn protein powder, 16-30 parts of flour, 0.5-1 part of vitamin premixed feed for the bullfrogs, 0.5-1 part of multi-mineral premixed feed for the bullfrogs and 0.4-0.8 part of bile acid in parts by weight, and also comprises the following fermented components in parts by weight: 1-3Kg of yeast, 1-2Kg of lactic acid composite bacteria, 1-2.5Kg of spore composite bacteria and 0.5-1Kg of complex enzyme. Also discloses a preparation method of the fermented feed capable of promoting the growth and development of bullfrogs. The invention has simple and convenient raw material acquisition and simple and feasible manufacturing method, can effectively solve the problems of heavy intestinal tract burden, unhealthy intestinal tract, weak disease resistance and the like caused by slow growth rate and low digestion rate of the bullfrogs in a high-density breeding mode, can obviously improve the specific growth rate of the bullfrogs during the breeding period, reduce the feed coefficient, reduce the dosage and greatly improve the breeding benefit.)

1. A fermented feed capable of promoting growth and development of bullfrogs is characterized by comprising the following components: fish meal, meat and bone meal, chicken meal, peanut meal, corn protein powder, flour, vitamin premixed feed for frogs, multi-mineral premixed feed for frogs and bile acid.

2. The fermented feed capable of promoting bullfrog growth and development according to claim 1, is characterized in that the weight portions of the components are 15-30 portions of fish meal, 5-10 portions of meat and bone meal, 10-20 portions of chicken meal, 17-30 portions of peanut meal, 5-12 portions of corn protein powder, 16-30 portions of flour, 0.5-1 portion of vitamin premixed feed for bullfrog, 0.5-1 portion of multi-mineral premixed feed for bullfrog and 0.4-0.8 portion of bile acid.

3. The fermented feed capable of promoting the growth and development of bullfrogs according to claim 1, further comprising the following fermentation components: yeast, lactic acid composite bacteria, spore composite bacteria and composite enzyme.

4. The fermented feed capable of promoting the growth and development of bullfrogs as claimed in claim 3, wherein the weight of each fermented component is as follows: 1-3Kg of yeast, 1-2Kg of lactic acid composite bacteria, 1-2.5Kg of spore composite bacteria and 0.5-1Kg of complex enzyme.

5. The fermented feed capable of promoting the growth and development of bullfrog according to claim 3, characterized in that the lactic acid complex bacteria comprise Pediococcus acidilactici and Lactobacillus acidophilus, the content of Pediococcus acidilactici is not less than 500 hundred million CFU/g, and the content of Lactobacillus acidophilus is not less than 500 hundred million CFU/g.

6. The fermented feed capable of promoting the growth and development of bullfrog according to claim 3, characterized in that the spore complex bacteria comprise bacillus subtilis and bacillus licheniformis, the content of the bacillus subtilis is not lower than 1000 hundred million CFU/g, and the content of the bacillus licheniformis is not lower than 500 hundred million CFU/g.

7. The fermented feed capable of promoting the growth and development of bullfrogs according to claim 3, wherein the complex enzyme comprises 10000U/g of acid protease, 5000U/g of neutral protease, 5000U/g of xylanase, 8000U/g of cellulase, 2000U/g of glucose oxidase and 5000U/g of pectinase.

8. The fermented feed capable of promoting the growth and development of bullfrogs according to claim 1, which is characterized by comprising the following components in parts by weight: 20 parts of seawater fish meal, 8 parts of meat and bone meal, 20 parts of chicken meal, 22 parts of peanut meal, 8 parts of corn protein powder, 20 parts of flour, 0.7 part of vitamin premixed feed for frogs, 0.7 part of multi-mineral premixed feed for frogs and 0.6 part of bile acid;

the fermentation liquid also comprises the following fermentation components in parts by weight: 3Kg of yeast, 1.8Kg of lactic acid composite bacteria, 2.2Kg of spore composite bacteria and 0.6Kg of complex enzyme.

9. The method for preparing a fermented feed for promoting the growth and development of bullfrogs according to any one of claims 1 to 8, comprising the steps of:

(1) granulating

Mixing the other components except the fermented component, and making into granule with particle size of 1.5-12 mm;

(2) preparation before fermentation

Dissolving the fermentation components in water to form a compound bacterial liquid;

(3) fermentation of

Atomizing and spraying the composite bacterial liquid onto the screened granules, stirring uniformly, carrying out aerobic fermentation for 15-30h, bagging, and carrying out anaerobic fermentation for 5-10 days to obtain the composite bacterial liquid.

10. The method for preparing fermented feed capable of promoting growth and development of bullfrogs according to claim 9, wherein the granules are prepared by puffing and granulating in the step (1); carrying out aerobic fermentation in a fermentation box; after the anaerobic fermentation is finished, subpackaging the fermented feed into plastic film bags with one-way valves, and sealing.

Technical Field

The invention relates to the field of special feed for bullfrogs, in particular to fermented feed capable of promoting growth and development of bullfrogs and a preparation method thereof.

Background

Bullfrog is the most famous edible frog in the world, and is named because its male ringing sound is like a cow. The bullfrog meat is white and tender, and has delicious taste and rich nutrition. Compared with chicken, duck, pig, cattle, sheep, etc., it is a nutritious health food with high protein, low fat, low calorie, and low cholesterol. Meanwhile, the nutrient also contains various micronutrients such as calcium, phosphorus, iron, thiamine, riboflavin, nicotinic acid and the like. According to modern traditional Chinese medicine, the bullfrog serving as a health food has the nourishing effects of tonifying yang, promoting diuresis, invigorating stomach, tonifying brain, tonifying deficiency, relieving cough and the like, can relieve hyperacidity, clear away heat and toxic materials, and has great effects on recovering physical fitness of people with physical weakness. The skin, skeleton and internal organs of bullfrog contain abundant collagen, and the bullfrog is a good food additive and dietary therapy tonic. In addition, from the culture perspective, the bullfrog culture technology has low requirement, the culture threshold is low, the feed coefficient of the bullfrog is low, the culture benefit is good, and the bullfrog is widely pursued by farmers. The existing bullfrog breeding industry develops rapidly, and breeding areas are mainly focused on Yuedong and Minnan.

However, the bullfrog breeding has some problems, and in the existing bullfrog breeding mode, the high-density breeding mode of deep water or semi-deep water is mainly used, so that the high-density breeding brings high income to farmers, and simultaneously brings the following problems:

1. the activity space of the bullfrog is compressed, and the growth speed of the bullfrog is reduced;

2. the high-density bred bullfrogs have poor living environment, low body resistance and low digestion level, are greedy to eat, and are infected by intestinal inflammation, so that other internal organs are affected to cause death;

3. the high-density culture causes the water quality to be poor, pathogens such as bacteria and the like are easy to breed, so that the bullfrogs are easy to catch diseases, and the dosage is very high.

The bullfrog feed appearing in the market at present mainly takes a bullfrog complete formula feed as a main material, and a functional feed is not reported. The common complete formula feed can meet the nutritional requirements of bullfrogs in all stages, but cannot solve the problems of low digestion level, slow growth speed and high dosage of the bullfrogs under high-density culture. This also restricts the further development of the bullfrog breeding industry.

Disclosure of Invention

In order to solve the technical problems, the invention aims to provide a fermented feed capable of promoting the growth and development of bullfrogs and a preparation method thereof, aiming at the problems of low digestion and utilization rate, slow growth speed, high dosage and the like of bullfrogs, and the fermented feed can improve the absorption efficiency of bullfrogs, enhance the immunity of frogs, improve the water quality and reduce the dosage.

In order to achieve the purpose, the invention provides the following technical scheme:

a fermented feed capable of promoting growth and development of bullfrogs comprises the following components: fish meal, meat and bone meal, chicken meal, peanut meal, corn protein powder, flour, vitamin premixed feed for frogs, multi-mineral premixed feed for frogs and bile acid.

As a preferred technical scheme, the feed comprises, by weight, 15-30 parts of fish meal, 5-10 parts of meat and bone meal, 10-20 parts of chicken meal, 17-30 parts of peanut meal, 5-12 parts of corn protein powder, 16-30 parts of flour, 0.5-1 part of vitamin premixed feed for frogs, 0.5-1 part of multi-mineral premixed feed for frogs and 0.4-0.8 part of bile acid.

As a preferred technical scheme, the fermentation medium further comprises the following fermentation components: yeast, lactic acid composite bacteria, spore composite bacteria and composite enzyme.

As a preferred technical scheme, the weight of each fermentation component is as follows: the weight of each fermentation component is as follows: 1-3Kg of yeast, 1-2Kg of lactic acid composite bacteria, 1-2.5Kg of spore composite bacteria and 0.5-1Kg of complex enzyme.

As a preferred technical scheme, the lactic acid complex bacteria comprise pediococcus acidilactici and lactobacillus acidophilus, wherein the content of the pediococcus acidilactici is not lower than 500 hundred million CFU/g, and the content of the lactobacillus acidophilus is not lower than 500 hundred million CFU/g.

As a preferred technical scheme, the spore compound bacteria comprise bacillus subtilis and bacillus licheniformis, wherein the content of the bacillus subtilis is not lower than 1000 hundred million CFU/g, and the content of the bacillus licheniformis is not lower than 500 hundred million CFU/g.

As a preferable technical scheme, the complex enzyme comprises 10000U/g acid protease, 5000U/g neutral protease, 5000U/g xylanase, 8000U/g cellulase, 2000U/g glucose oxidase and 5000U/g pectinase.

As a preferred technical scheme, the fish meal is seawater fish meal.

As a preferable technical scheme, the fermented feed capable of promoting the growth and development of bullfrogs comprises the following components in parts by weight: 20 parts of seawater fish meal, 8 parts of meat and bone meal, 20 parts of chicken meal, 22 parts of peanut meal, 8 parts of corn protein powder, 20 parts of flour, 0.7 part of vitamin premixed feed for frogs, 0.7 part of multi-mineral premixed feed for frogs and 0.6 part of bile acid;

the fermentation liquid also comprises the following fermentation components in parts by weight: 3Kg of yeast, 1.8Kg of lactic acid composite bacteria, 2.2Kg of spore composite bacteria and 0.6Kg of complex enzyme.

The invention also provides a preparation method of the fermented feed capable of promoting the growth and development of bullfrogs, which specifically comprises the following steps:

(1) granulating

Mixing the above components except for the fermented component, and making into granule with particle size of 1.5-12 mm;

(2) preparation before fermentation

Dissolving the fermentation components in water to form a compound bacterial liquid;

(3) fermentation of

Atomizing and spraying the composite bacterial liquid onto the screened granules, stirring uniformly, carrying out aerobic fermentation for 15-30h, bagging, and carrying out anaerobic fermentation for 5-10 days to obtain the composite bacterial liquid.

As a preferable technical scheme, the granules are prepared by puffing and granulating in the step (1);

carrying out aerobic fermentation in a fermentation box; after the anaerobic fermentation is finished, the fermented feed is subpackaged into plastic film bags with one-way valves, and the bags are sealed so as to be convenient to store.

Compared with the prior art, the invention has the following advantages:

a. while meeting the daily nutritional basic requirements of bullfrogs, the anti-nutritional factors in the feed are greatly reduced through a fermentation process, the absorption efficiency of the bullfrogs can be improved, the growth rate is accelerated, and meanwhile, the feed has a large amount of beneficial bacteria and fermentation metabolites, so that the immunity and the antibacterial capability of the frogs can be enhanced, and the dosage is reduced;

b. compared with the common complete bullfrog compound feed, the invention uses the viable bacteria for solid fermentation, greatly reduces the anti-nutritional factors in the feed, simultaneously degrades some macromolecular substances which can not be digested and absorbed by the bullfrog, can improve the digestion and utilization rate of the bullfrog on the feed, and quickens the growth rate of the bullfrog;

c. compared with the common complete bullfrog compound feed, various active probiotics contained in the feed can form probiotic groups in the bullfrog intestinal tract, improve the intestinal health level, acidify the intestinal tract and resist the invasion of harmful bacteria; the fermentation metabolite contains various oligosaccharides, small peptides, vitamins and unknown growth factors, so that the immunity level of bullfrogs can be improved, the disease resistance is improved, and the dosage is reduced;

d. compared with the common complete bullfrog compound feed, the compound feed has a large amount of live bacteria, so that the microorganism on the bullfrog excrement and the feed residual bait is greatly increased, the decomposition and the conversion of the excrement and the feed residual bait are accelerated, the water quality is improved, the opportunity of infecting germs by the bullfrog is reduced to a greater extent, and a good environment is provided for the growth and the development of the bullfrog;

e. the fermented feed disclosed by the invention is stable in raw material source, relatively low in cost and simple in preparation process, and can meet the nutritional requirements of bullfrog in each growth stage;

f. the selected strains are easy to obtain, the cost is low, the fermentation effect is satisfactory, the granulated feed can be fully degraded, macromolecular substances and anti-nutritional factors in feed raw materials are reduced to a greater extent, and a large amount of beneficial metabolites are generated.

Drawings

FIG. 1 is a schematic representation of the digestion of the intragastric feed in each group three hours after experiment 1;

FIG. 2 is a schematic representation of the digestion of the intragastric feed in each group six hours after test example 1;

FIG. 3 is a schematic diagram showing the digestion of the intragastric feed in each group nine hours after experiment 1;

FIG. 4 is a schematic view of the intestinal section of bullfrog in the control group in Experimental example 2;

FIG. 5 is a schematic view of sliced intestinal tracts of bullfrog in Experimental group of Experimental example 2.

Detailed Description

The present invention will be described in further detail with reference to examples, but the embodiments of the present invention are not limited thereto. The reagents, equipment and methods adopted by the invention are all reagents, equipment and methods which are conventionally and commercially available in the technical field and are conventionally used in the technical field.