阅读说明：本技术 一种高抑菌性葡萄籽提取物的制备方法 (Preparation method of grape seed extract with high antibacterial activity ) 是由 缪文华 汪舟娜 于 2019-09-29 设计创作，主要内容包括：本发明涉及植物提取物改性技术领域,公开了一种高抑菌性葡萄籽提取物的制备方法,步骤为：将葡萄籽粉碎过筛得到葡萄籽粉末；向葡萄籽粉末中加入乙醇,超声提取得到葡萄籽提取液；将葡萄籽提取液真空干燥后得到葡萄籽提取物；用低温等离子体气氛处理葡萄籽提取物,得到高抑菌性葡萄籽提取物。本发明利用低温等离子体的高能粒子和活性组分与葡萄籽提取物表面发生作用,可以对葡萄籽提取物中的多酚类等成分表面进行修饰改性,从而有效提高了葡萄籽提取物的抑菌活性。(The invention relates to the technical field of plant extract modification, and discloses a preparation method of a high antibacterial activity grape seed extract, which comprises the following steps: crushing and sieving grape seeds to obtain grape seed powder; adding ethanol into the grape seed powder, and performing ultrasonic extraction to obtain a grape seed extracting solution; vacuum drying the grape seed extract to obtain a grape seed extract; treating the grape seed extract with low temperature plasma atmosphere to obtain grape seed extract with high antibacterial activity. The invention utilizes the action of high-energy particles and active components of low-temperature plasma on the surface of the grape seed extract, and can modify the surfaces of components such as polyphenols in the grape seed extract, thereby effectively improving the bacteriostatic activity of the grape seed extract.)

1. A preparation method of a grape seed extract with high antibacterial activity is characterized by comprising the following steps:

(1) crushing and sieving grape seeds to obtain grape seed powder;

(2) adding ethanol into the grape seed powder, and performing ultrasonic extraction to obtain a grape seed extracting solution;

(3) vacuum drying the grape seed extract to obtain a grape seed extract;

(4) treating the grape seed extract with low temperature plasma atmosphere to obtain grape seed extract with high antibacterial activity.

2. The method for preparing grape seed extract with high antibacterial activity as claimed in claim 1, wherein the working gas for generating the low temperature plasma atmosphere in step (4) is O with a volume ratio of 1:1 to 3:1₂And N₂。

3. The method for preparing a grape seed extract with high bacteriostatic activity according to claim 1 or 2, wherein the treatment power in step (4) is 600-800W.

4. The method for preparing a grape seed extract with high bacteriostatic activity according to claim 1 or 2, wherein the treatment time in step (4) is 10-15 min.

5. The method for preparing the grape seed extract with high bacteriostatic activity according to claim 1, wherein the grape seed extract is sieved by a 60-100 mesh sieve in the step (1).

6. The method for preparing a grape seed extract with high bacteriostatic activity according to claim 1, wherein the ethanol is added in the step (2) in a feed-liquid ratio of 1: (7-9), wherein the volume fraction of the ethanol is 40-60%.

7. The method for preparing the grape seed extract with high antibacterial activity according to claim 1 or 6, wherein the ultrasonic frequency in step (2) is 35-45 Hz, the ultrasonic time is 40-50 min, and the extraction temperature is 35-55 ℃.

8. The method for preparing a grape seed extract with high bacteriostatic activity according to claim 1, wherein the vacuum drying temperature in step (3) is 60-80 ℃.

Technical Field

The invention relates to the technical field of plant extract modification, in particular to a preparation method of a grape seed extract with high antibacterial activity.

Background

Grapes are one of the fruits commonly cultivated in the world, and grapes are produced in China for about 140 thousands of tons, and about 80 percent of the grapes are used for brewing wine. Grape seeds are a by-product of the wine brewing industry, and during wine brewing, 3% of the total amount of grapes are produced.

Grape seeds contain abundant polyphenols and have certain antibacterial and antioxidant capabilities, so that the grape seed extract rich in polyphenols is widely applied to the fields of foods, medicines, cosmetics and the like for bacteriostasis. For example, in chinese patent literature, "a method for inhibiting bacteria and preserving fresh prepared pork by using compound plant polyphenol", publication No. CN109042830A, the invention inhibits bacteria in fresh prepared pork by using tea polyphenol and grape seed extract, can significantly inhibit the growth of cyclosporine, pseudomonas, enterobacter and staphylococcus in the storage process of fresh prepared pork, significantly inhibit volatile amino nitrogen, and can maintain the pH, hardness and a value of fresh prepared pork in the storage process, and maintain the storage quality of the product.

However, the bacteriostatic performance of the grape seed extract is still to be improved, and the grape seed extract is required to be compounded with other components to achieve the use requirement when being used as a bacteriostatic agent, so that the finding of a method for effectively improving the grape seed extract is of great significance.

Disclosure of Invention

The invention provides a preparation method of a high-antibacterial-activity grape seed extract, aiming at overcoming the problems that grape seeds contain abundant polyphenols, have certain antibacterial activity, can be widely applied to the fields of food, medicine, cosmetics and the like for bacteriostasis, but the antibacterial performance of the grape seeds is still to be improved, and the use requirement can be met only by compounding the grape seeds with other components to realize the antibacterial effect.

In order to achieve the purpose, the invention adopts the following technical scheme:

a preparation method of a grape seed extract with high antibacterial activity comprises the following steps:

(1) crushing and sieving grape seeds to obtain grape seed powder;

(2) adding ethanol into the grape seed powder, and performing ultrasonic extraction to obtain a grape seed extracting solution;

(3) vacuum drying the grape seed extract to obtain a grape seed extract;

(4) treating the grape seed extract with low temperature plasma atmosphere to obtain grape seed extract with high antibacterial activity.

In the steps (1) to (3), the grape seed extract is obtained by using ethanol as a solvent and adopting an ultrasonic extraction method, the penetration capacity of the ethanol to plant cells is also stronger, and the polyphenols in the grape seeds have a polyphenol hydroxyl structure and certain polarity and are easily dissolved in the hydrophilic organic solvent ethanol, so that the polyphenols can be effectively extracted; ethanol-insoluble substances such as starch, protein, pectin, and mucilage cannot be extracted, so the obtained grape seed extract contains polyphenols as main ingredient and has antibacterial effect.

And (4) treating the grape seed extract by using a low-temperature plasma technology in the step (4). The plasma is a fourth state of a substance under natural conditions, is an aggregate containing ions, electrons, neutral atoms (ground state or excited state), free radicals and other particles generated by ionizing gas molecules under the excitation of energy such as radiation, heat or applied electric field, and can generate an electromagnetic field with chemical bond breaking capacity, heat, ultraviolet rays, charged particles, excited state particles, metastable state particles and other active components in the generation process, and the surfaces of polyphenols and other components in the grape seed extract can be modified by utilizing the action of the high-energy particles and the active components of the low-temperature plasma and the surfaces of the grape seed extract, for example, quinone substances and other substances are generated, so that the antibacterial activity of the grape seed extract is improved.

Preferably, the working gas for generating the low-temperature plasma atmosphere in the step (4) is O with the volume ratio of 1: 1-3: 1₂And N₂. Under the atmosphere, polyphenol components in the grape seed extract can be effectively modified to generate quinones and other substances, so that the antibacterial activity of the grape seed extract is improved.

Preferably, the processing power in the step (4) is 600-800W.

Preferably, the treatment time in the step (4) is 10-15 min. Under the treatment power and time of the low-temperature plasma, the obtained product has the best antibacterial performance.

Preferably, the step (1) is carried out by sieving with a 60-100 mesh sieve. The grape seed powder with the particle size range is beneficial to extracting polyphenol components in the grape seed powder, so that the antibacterial performance of the grape seed extract is improved.

Preferably, the feed-liquid ratio of ethanol added in the step (2) is 1: (7-9), wherein the volume fraction of the ethanol is 40-60%. The polyphenol substances in the grape seed powder cannot be effectively extracted due to too low concentration of the ethanol, the polarity difference between the solvent and the polyphenol is increased due to too high concentration of the ethanol, the elution amount of lipophilic components such as alcohol-soluble impurities is increased, and the lipophilic components compete with the polyphenol substances to combine with the solvent, so that the extraction rate of the polyphenol substances is reduced, and the antibacterial performance of the grape seed extract is reduced.

Preferably, in the step (2), the ultrasonic frequency is 35-45 Hz, the ultrasonic time is 40-50 min, and the extraction temperature is 35-55 ℃. Under the ultrasonic condition and temperature, the effective components in the grape seeds can be ensured not to be damaged and can be fully extracted.

Preferably, the vacuum drying temperature in the step (3) is 60-80 ℃.

Therefore, the invention has the following beneficial effects: the grape seed extract obtained by using ethanol as a solvent and adopting an ultrasonic extraction method has high polyphenol content and certain bacteriostatic property, and then high-energy particles and active components of low-temperature plasma are utilized to react with the surface of the grape seed extract, so that the surfaces of the polyphenols and other components in the grape seed extract can be modified, such as quinone and other substances are generated, and the bacteriostatic activity of the grape seed extract is effectively improved.

Detailed Description

The invention is further described with reference to specific embodiments.