阅读说明：本技术 一种具有抗菌活性的金樱子果实粗多糖制备方法 (Preparation method of cherokee rose fruit crude polysaccharide with antibacterial activity ) 是由 张立新 岳丹丹 刘学贵 高品一 李丹琦 于 2019-10-24 设计创作，主要内容包括：一种具有抗菌活性的金樱子果实粗多糖制备方法,涉及一种抗菌活性多糖制备方法,通过以下制备步骤得到的：将金樱子干燥的果实,经粉碎机粉碎过筛后用石油醚、乙醇回流处理,去除脂质和含有的单糖、低聚糖以及皂苷类等干扰化学成分；然后选取微波功率(450-550W)、提取时间(10-20min)、提取次数(1-5次)以及料液比(1:40-1:60g/mL)对金樱子果实多糖提取,提取液减压浓缩干燥得到金樱子果实粗多糖。该方法取金樱子干燥的果实经除去样品中含有的单糖、低聚糖以及皂苷类等干扰化学成分,表明在多糖浓度为100-200mg/mL时显示出明显的金黄色葡萄球菌抑制作用,为进一步药物开发研究奠定了基础。(A preparation method of cherokee rose fruit crude polysaccharide with antibacterial activity relates to a preparation method of polysaccharide with antibacterial activity, which is obtained by the following preparation steps: pulverizing dried fructus Rosae Laevigatae, sieving, and reflux treating with petroleum ether and ethanol to remove lipid, monosaccharide, oligosaccharide and saponin; then selecting microwave power (450-. According to the method, the interference chemical components such as monosaccharide, oligosaccharide and saponins contained in the sample of dried cherokee rose fruit are removed, which shows that the method shows obvious staphylococcus aureus inhibition effect when the polysaccharide concentration is 100-200mg/mL, and lays a foundation for further drug development and research.)

1. The preparation method of the cherokee rose fruit crude polysaccharide with antibacterial activity is characterized in that the cherokee rose fruit crude polysaccharide with antibacterial activity is obtained by the following preparation steps:

pulverizing dried fructus Rosae Laevigatae, sieving, and reflux treating with petroleum ether and ethanol to remove lipid, monosaccharide, oligosaccharide and saponin; then combining the extracting solutions, and obtaining a crude product after decompression, concentration and drying; pulverizing dried fructus Rosae Laevigatae, and sieving to obtain fructus Rosae Laevigatae powder; adding 1000 ml of 500-1000 ml of petroleum ether into the cherokee rose fruit powder, heating in a water bath kettle, and controlling the temperature to be 60-90 DEG^oC, refluxing, extracting and degreasing for 6-12 h; after the sample volatilizes the reagent, 500-1000 ml of ethanol with the volume fraction of 60-90 percent is added into the sample to be mixed in the mixture of 70-90 percent^oC, carrying out reflux extraction for 2-5 h at the temperature of C, and removing interference chemical components such as monosaccharide, oligosaccharide and saponins contained in the sample; pouring out the liquid on the upper layer, and drying the sample to balance weight; optimization extraction tool by adopting response surface design method on the basis of single-factor experimentAfter the process, the polysaccharide of the cherokee rose fruit is extracted by selecting microwave power (450-.

Technical Field

The invention relates to a preparation method of polysaccharide with antibacterial activity, in particular to a preparation method of cherokee rose fruit crude polysaccharide with antibacterial activity.

Background

The medicinal value of cherokee rose fruit is as follows: chinese medicine cherokee rose fruitRosa laevigataMichx, a plant of the rosa genus of the rosaceae family, is widely distributed in China. All the fruits, roots and leaves mentioned in Shu Ben Cao (materia Medica of Shu). Cherokee rose fruit is recorded in Ben Cao gang mu: sour, astringent, mild and nontoxic; mainly treats spleen diarrhea and diarrhea, stops small, convenient and astringent. Mature fruits of the traditional Chinese medicine cherokee rose contain abundant polysaccharide, flavone and triterpenoid saponin compounds, so that the cherokee rose fruit has various biological activities of improving the organism immunity, resisting tumors, bacteria, stress and inflammation, reducing blood sugar, blood pressure, blood fat, arrhythmia and the like. The cherokee rose fruit as a good medicinal product has sweet taste and fragrant smell, and has extremely wide development and production prospects in the fields of food and medicine.

The crude polysaccharide of the cherokee rose fruit is extracted from dried cherokee rose fruit, and has proven biological activities of protecting liver, resisting oxidation, inhibiting bacteria, resisting inflammation, resisting tumor, enhancing non-specific immune function of organism and the like, so the polysaccharide has wide development prospect in the fields of food, industry, pharmaceutical research and development and the like for deeply exploring other pharmacological activities of the cherokee rose fruit polysaccharide.

Disclosure of Invention

The invention aims to provide a preparation method of cherokee rose fruit crude polysaccharide with antibacterial activity, which removes interference chemical components such as monosaccharide, oligosaccharide and saponins contained in a sample from cherokee rose fruit dried fruit, and shows that the cherokee rose fruit crude polysaccharide shows obvious staphylococcus aureus inhibition effect when the polysaccharide concentration is 100-200mg/mL, thereby laying a foundation for further drug development and research.

The purpose of the invention is realized by the following technical scheme:

a preparation method of cherokee rose fruit crude polysaccharide with antibacterial activity is provided, wherein the cherokee rose fruit crude polysaccharide with antibacterial activity is obtained by the following preparation steps:

pulverizing dried fructus Rosae Laevigatae, sieving, and reflux treating with petroleum ether and ethanol to remove lipid, monosaccharide, oligosaccharide and saponin; then combining the extracting solutions, and obtaining a crude product after decompression, concentration and drying; pulverizing dried fructus Rosae Laevigatae, and sieving to obtain fructus Rosae Laevigatae powder; adding 1000 ml of 500-1000 ml of petroleum ether into the powder of the cherokee rose fruit in waterHeating in bath pot at 60-90 deg.C^oC, refluxing, extracting and degreasing for 6-12 h; after the sample volatilizes the reagent, 500-1000 ml of ethanol with the volume fraction of 60-90 percent is added into the sample to be mixed in the mixture of 70-90 percent^oC, carrying out reflux extraction for 2-5 h at the temperature of C, and removing interference chemical components such as monosaccharide, oligosaccharide and saponins contained in the sample; pouring out the liquid on the upper layer, and drying the sample to balance weight; after the extraction process is optimized by adopting a response surface design method on the basis of a single-factor experiment, the polysaccharide of the cherokee rose fruit is extracted by selecting microwave power (450-550W), extraction time (10-20min), extraction times (1-5 times) and a material-liquid ratio (1: 40-1:60 g/mL), and the extracting solution is decompressed, concentrated and dried to obtain the crude polysaccharide of the cherokee rose fruit. The invention has the advantages and effects that:

the antibacterial activity research result of the crude cherokee rose fruit polysaccharide prepared by the invention shows that the polysaccharide concentration is 100-200mg/mL and shows obvious staphylococcus aureus inhibition, and the polysaccharide concentration is 100-300 mg/mL and shows obvious escherichia coli inhibition.

Therefore, the cherokee rose fruit crude polysaccharide prepared by the invention has certain antibacterial activity, and lays a foundation for further development and research of related medicaments.

Drawings

FIG. 1 is a graph showing the effect of the antibacterial activity test of the crude polysaccharide of cherokee rose fruit.

Detailed Description

The present invention will be described in detail with reference to examples.

The present invention will be described below with reference to specific examples, but the present invention is not limited thereto. The experimental methods described in the following examples are all conventional methods unless otherwise specified; the reagents and biomaterials, if not specifically indicated, are commercially available.