阅读说明：本技术 动物养殖用复合益生菌发酵饲料及其制备方法 (Composite probiotic fermented feed for animal breeding and preparation method thereof ) 是由 谈春季 李桂霞 范东良 李瑞忠 安电进 刘玉珍 于 2019-10-30 设计创作，主要内容包括：本发明公开了一种动物养殖用复合益生菌发酵饲料及其制备方法,包括固体培养基和复合益生菌菌液,以重量份计,所述固体培养基包括米糠20-40份,玉米喷浆蛋白10-25份,玉米粉1-5份,次粉3-10份,DDGS1-5份,麸皮5-10份,氯化钠0.1-0.5份；所述复合益生菌菌液为20-35份；本发明制备方法是将各组份粉碎、混合、发酵,发酵条件为厌氧,温度30-45℃,时间10-30天。本发明的发酵饲料能够有效提高猪、禽和反刍采食量、增加饲料的转化利用率、减少畜舍氨气排放量、增强母畜繁殖能力、提高奶水质量等,且本发明配方成本低廉,固体培养基好获；本发明的制备方法简单,能有效解决成品封装后的二次发酵、包装胀包问题。本发明制备的复合益生菌发酵饲料适用于养殖猪、禽和反刍类等通用的物种。(The invention discloses a composite probiotic fermented feed for animal breeding and a preparation method thereof, wherein the feed comprises a solid culture medium and a composite probiotic bacterial liquid, and the solid culture medium comprises, by weight, 20-40 parts of rice bran, 10-25 parts of corn guniting protein, 1-5 parts of corn flour, 3-10 parts of wheat middling, 1-5 parts of DDGS, 5-10 parts of bran and 0.1-0.5 part of sodium chloride; 20-35 parts of the composite probiotic bacterial liquid; the preparation method comprises the steps of crushing, mixing and fermenting the components, wherein the fermentation condition is anaerobic, the temperature is 30-45 ℃, and the time is 10-30 days. The fermented feed can effectively improve the feed intake of pigs, poultry and ruminants, increase the conversion utilization rate of the feed, reduce the discharge amount of ammonia gas in animal houses, enhance the reproductive capacity of female animals, improve the quality of milk and the like, and has low formula cost and good solid culture medium acquisition; the preparation method is simple, and can effectively solve the problems of secondary fermentation and package expansion after the finished product is packaged. The composite probiotic fermented feed prepared by the invention is suitable for breeding general species such as pigs, poultry and ruminants.)

1. A composite probiotic fermented feed for animal breeding is characterized in that: the probiotic bacteria liquid culture medium comprises a solid culture medium and a compound probiotic bacteria liquid, wherein the solid culture medium comprises the following components in parts by weight: 20-40 parts of rice bran, 10-25 parts of corn guniting protein, 1-5 parts of corn flour, 3-10 parts of wheat middling, 5-5 parts of DDGS1, 5-10 parts of bran and 0.1-0.5 part of sodium chloride; 20-35 parts of composite probiotic bacterial liquid.

2. The composite probiotic fermented feed for animal breeding according to claim 1, characterized in that: the composite probiotic bacteria liquid comprises lactobacillus plantarum bacteria liquid, saccharomyces cerevisiae bacteria liquid and bacillus subtilis bacteria liquid.

3. The composite probiotic fermented feed for animal breeding according to claim 2, characterized in that: the viable count of the lactobacillus plantarum in the composite probiotic bacterial liquid is more than or equal to 1 multiplied by 10⁸CFU/ml, the number of viable bacteria of Saccharomyces cerevisiae is not less than 1 × 10⁸CFU/ml, viable count of Bacillus subtilis is not less than 1 × 10⁹CFU/ml。

4. The composite probiotic fermented feed for animal breeding according to any one of claims 1 to 3, characterized in that: in the solid culture medium, 30 parts of rice bran, 18 parts of corn guniting protein, 3 parts of corn flour, 6 parts of wheat middling, 3 parts of DDGS, 8 parts of bran, 0.3 part of sodium chloride and 28 parts of composite probiotic bacterial liquid.

5. A method for preparing the composite probiotic fermented feed for animal breeding according to any one of claims 1 to 4, which is characterized by comprising the following steps: the preparation method comprises the following steps in sequence:

the method comprises the following steps: weighing the components in the solid culture medium, and crushing and mixing;

step two: adding the composite probiotic bacteria liquid into the mixed solid culture medium, and mixing to form a mixture;

step three: fermenting the mixture under the following conditions: anaerobic treatment at 30-45 deg.c for 10-30 days.

6. The preparation method of the composite probiotic fermented feed for animal breeding according to claim 5, characterized by comprising the following steps: in the first step, all components of the solid culture medium are crushed by a crusher with a sieve sheet diameter of 1.0 mm.

7. The method for preparing the composite probiotic fermented feed for animal breeding according to claim 6, characterized by comprising the following steps: in the first step, the time for mixing the components after crushing is 60-120 s.

8. The method for preparing the composite probiotic fermented feed for animal breeding according to any one of claims 5 to 7, characterized in that: in the second step, the time for mixing after adding the composite probiotic bacteria liquid is 120-180 s.

Technical Field

The invention belongs to the technical field of animal nutrition and feed, relates to a feed for breeding and a preparation method of the feed for breeding, and particularly relates to a composite probiotic fermented feed for animal breeding and a preparation method of the feed.

Background

With the increasing global population, the demand of food is increasing, so the feeding of modern livestock and poultry tends to large-scale centralized breeding, and various feeds and nutritional means are required to improve and increase the growth speed in the feeding process.

However, the shortage of feed resources and the effective utilization of unconventional raw materials are main factors restricting the further development of feed industry and animal husbandry in China. In order to solve the problem of shortage of feed resources, research and development of high-quality novel feed are required. For example, a bio-fermented feed sold on the market at present is a novel feed, which is fermented by microorganisms, so that the fermented feed contains more active probiotic bacteria, various enzymes, metabolites of each level, vitamins, protein decomposition products, active small peptides, amino acids, antibacterial substances, immune enhancement factors, growth promotion factors and the like, and can play a role in promoting growth and maintaining the balance of animal intestinal flora. Meanwhile, the feed can be digested in vitro through the fermentation of microorganisms, and then the feed can be better absorbed by animals. In addition, the feed obtained by biological fermentation is not added with any antibiotic, so that antibiotic residue is avoided, and the feed is safer for consumers to eat.

Although the fermented feed has the advantages, the popularization range of the fermented feed in the prior art is still relatively small, because microorganisms have activity, and the fermented feed is easy to generate fermentation gas in the transportation and storage processes by using the existing fermentation means and packaging means, so that the packaging bag is expanded, and the sale of the fermented feed is influenced. In order to prevent bag expansion, the external expiratory valve is arranged in the prior art, so that gas generated in the packaging bag is exhaled out of the packaging bag, and external air cannot enter the packaging bag.

Vacuum packaging is a packaging method which is recently advocated, and not only is the packaging simple and convenient, but also the storage period of the products can be prolonged, and if the vacuum packaging is applied to the packaging of fermented feed, the packaging problem of the fermented feed can be solved, but the fermented feed in the prior art uses a fermentation substrate and a fermentation process, and the bag expansion happens as usual when the vacuum packaging is adopted, so that the bag expansion cannot be avoided.

In addition, most of the feed in the prior art has high protein content, the protein conversion rate is low after the feed is used by animals, and a large amount of ammonia gas can be discharged, wherein the ammonia gas is a toxic gas, so that the feed has great influence on animal breeding and causes the problem of environmental pollution. Excessive ammonia in the air can cause cough, congestion and edema of respiratory mucosa of livestock and poultry, thereby causing conjunctivitis, bronchitis, pneumonia and pulmonary edema, and simultaneously, the ammonia can also act on a nervous system after entering blood, thereby causing nervous excitation and paralysis of the nervous system, so high-concentration ammonia in a livestock shed can increase the morbidity of animals. Therefore, the fermented feed which can reduce the discharge amount of ammonia gas after being eaten by animals and is suitable for vacuum packaging is provided, and is a great progress for the feed processing industry and the breeding industry.

Disclosure of Invention

The technical problem to be solved by the invention is to provide a microbial fermentation feed suitable for vacuum packaging so as to improve the feed intake of pigs, increase the feed conversion rate and prevent the secondary fermentation of the feed in the storage and transportation processes and the bag expansion of the package; the invention aims to solve another technical problem and provide a preparation method of fermented feed so as to achieve the aim of convenient operation in the preparation process.

In order to achieve the purpose, the invention adopts the following technical scheme: the composite probiotic fermented feed for animal breeding comprises a solid culture medium and composite probiotic bacteria liquid, wherein the solid culture medium comprises the following components in parts by weight: 20-40 parts of rice bran, 10-25 parts of corn guniting protein, 1-5 parts of corn flour, 3-10 parts of wheat middling, 5-5 parts of DDGS1, 5-10 parts of bran and 0.1-0.5 part of sodium chloride; 20-35 parts of composite probiotic bacterial liquid.

As a limitation of the present invention: the composite probiotic bacteria liquid comprises lactobacillus plantarum bacteria liquid, saccharomyces cerevisiae bacteria liquid and bacillus subtilis bacteria liquid.

As a limitation of the present invention: the viable count of the lactobacillus plantarum in the composite probiotic bacterial liquid is more than or equal to 1 multiplied by 10⁸CFU/ml, the number of viable bacteria of Saccharomyces cerevisiae is not less than 1 × 10⁸CFU/ml, viable count of Bacillus subtilis is not less than 1 × 10⁹CFU/ml。

As another limitation of the present invention: in the solid culture medium, 30 parts of rice bran, 18 parts of corn guniting protein, 3 parts of corn flour, 6 parts of wheat middling, 3 parts of DDGS, 8 parts of bran, 0.3 part of sodium chloride and 28 parts of composite probiotic bacterial liquid.

The invention also provides a preparation method of the composite probiotic fermented feed for animal breeding, which is carried out according to the following steps in sequence:

the method comprises the following steps: weighing the components in the solid culture medium, and crushing and mixing;

step two: adding the composite probiotic bacteria liquid into the mixed solid culture medium, and mixing to form a mixture;

step three: fermenting the mixture under the following conditions: anaerobic treatment at 30-45 deg.c for 10-30 days.

As the limit to the preparation method of the composite probiotic fermented feed for animal breeding, the invention comprises the following steps: in the first step, all components of the solid culture medium are crushed by using a crusher with a sieve sheet diameter of 1.0 mm.

As a limitation to the preparation method of the composite probiotic fermented feed for animal breeding of the invention: in the first step, the time for mixing the components after crushing is 60-120 s.

As another limitation to the preparation method of the composite probiotic fermented feed for animal breeding of the present invention: in the second step, the time for mixing after adding the composite probiotic bacteria liquid is 120-180 s.

Due to the adoption of the technical scheme, compared with the prior art, the invention has the following beneficial effects:

(1) according to the invention, sodium chloride is added into the solid culture medium, and the composite probiotics are utilized to ferment the solid culture medium, so that the feed is suitable for vacuum packaging, the feed is prevented from bag expansion in the transportation and storage processes, and the quality guarantee period of the feed is prolonged;

(2) the fermented feed obtained by the invention can effectively improve the feed intake of livestock and poultry, increase the conversion utilization rate of the feed, enhance the reproductive capacity of the female livestock, improve the milk quality and the like, and probiotics and metabolites in the fermented feed can increase the digestibility of protein in the feed, so that the protein content in excrement is reduced, and the emission of ammonia in a livestock house can be effectively reduced;

(3) the invention has low formula cost, easily obtained feed raw materials and simple preparation method, and can effectively solve the problem of packing flatulence by controlling the fermentation temperature and the fermentation time through the combination of the fermentation solid culture medium, thereby being better suitable for vacuum packing and facilitating the transportation and storage of the fermented feed.

The composite probiotic fermented feed prepared by the invention is suitable for breeding general species such as pigs, poultry and ruminants.

Detailed Description

The present invention will be described in further detail with reference to examples.