阅读说明：本技术 Pcr引物对及其应用 (PCR primer pair and application thereof ) 是由 杨林 蒋浩君 曾鹏 庄雪寒 高雅 张艳艳 蒋慧 郭晶 陈芳 徐讯 于 2017-06-20 设计创作，主要内容包括：提供了PCR引物对及其应用。其中,该PCR引物对包括：第一引物和第二引物,其中,所述第一引物包含第一特异性序列、第一随机序列和第一通用序列,所述第一特异性序列位于所述第一引物的3’端,所述第一随机序列位于所述第一引物的5’端,所述第一通用序列位于所述第一特异性序列和所述第一随机序列之间,所述第二引物包含第二特异性序列、第二随机序列和第二通用序列,所述第二特异性序列位于所述第二引物的3’端,所述第二随机序列位于所述第二引物的5’端,所述第二通用序列位于所述第二特异性序列和所述第二随机序列之间,其中,所述第一随机序列和所述第二随机序列反向互补。(PCR primer pairs and uses thereof are provided. Wherein, the PCR primer pair comprises: a first primer and a second primer, wherein the first primer comprises a first specific sequence, a first random sequence and a first universal sequence, the first specific sequence is located at the 3 'end of the first primer, the first random sequence is located at the 5' end of the first primer, the first universal sequence is located between the first specific sequence and the first random sequence, the second primer comprises a second specific sequence, a second random sequence and a second universal sequence, the second specific sequence is located at the 3 'end of the second primer, the second random sequence is located at the 5' end of the second primer, the second universal sequence is located between the second specific sequence and the second random sequence, and the first random sequence and the second random sequence are in reverse complementarity.)