阅读说明：本技术 一种冷等离子体处理的双精氨酸抗菌剂及制备方法和应用 (Cold plasma treated bis-arginine antibacterial agent, preparation method and application ) 是由 林琳 廖雪 崔海英 朱玉林 于 2019-08-30 设计创作，主要内容包括：本发明属于食品安全领域,具体涉及一种冷等离子体处理的双精氨酸抗菌剂及制备方法和应用。利用双精氨酸的优良抗菌效果,并通过冷等离子体对双精氨酸粉末进行表面改性,进一步提高抗菌效果,制备一种安全绿色、无毒副作用新型抗菌剂。该种新型抗菌剂能有效杀死各种食源性病原菌,院内感染菌如耐甲氧西林金黄色葡萄球菌等,与不处理的双精氨酸相比,冷等离子体处理的双精氨酸在磷酸缓冲液和肉汤中的抗菌效果都有了明显的提升。(The invention belongs to the field of food safety, and particularly relates to a double-arginine antibacterial agent treated by cold plasma, and a preparation method and application thereof. The excellent antibacterial effect of the double arginine is utilized, the cold plasma is used for carrying out surface modification on the double arginine powder, the antibacterial effect is further improved, and the novel safe, green and toxic-side-effect-free antibacterial agent is prepared. The novel antibacterial agent can effectively kill various food-borne pathogenic bacteria and nosocomial infectious bacteria such as methicillin-resistant staphylococcus aureus and the like, and compared with untreated double arginine, the antibacterial effect of the cold plasma treated double arginine in phosphate buffer solution and broth is obviously improved.)

1. The cold plasma treated double arginine antibacterial agent is characterized by being prepared by the following method: firstly, processing the double-arginine solid powder by using cold plasma to obtain cold plasma modified double-arginine; and adding the arginines into the acetic acid solution, and fully stirring to dissolve the arginines to obtain the arginines acetic acid solution, namely the antibacterial agent.

2. The plasma-processed di-arginine antibacterial agent as claimed in claim 1, wherein the cold plasma uses a mixed gas of nitrogen and oxygen as an exciting gas, the flow rate is 100sccm, the processing time of the cold plasma is 3-5min, and the processing power is 300-.

3. A cold plasma treated bis-arginine antimicrobial agent according to claim 2, wherein the volume ratio of nitrogen to oxygen is 1: 1.

4. A cold plasma treated bis-arginine antibacterial agent according to claim 1, wherein in the bis-arginine acetic acid solution: the concentration of the double arginine is 2-10mg/mL, and the volume percentage concentration of the acetic acid is 1% -5%.

5. The cold plasma treated bis-arginine antimicrobial agent according to claim 1, wherein the stirring is performed under a magnetic stirring condition at a rotation speed of 25r/min for 30 min.

6. Use of a cold plasma treated bis-arginine antibacterial agent as claimed in any one of claims 1 to 5 as an antibacterial agent in the field of food safety against microbial contamination in food.

7. The use of claim 6, wherein the bacteria comprise Salmonella and methicillin-resistant Staphylococcus aureus.

Technical Field

The invention belongs to the field of food safety, and particularly relates to a double-arginine antibacterial agent treated by cold plasma, and a preparation method and application thereof.

Background

The existing antibacterial agents are various, and most of the existing antibacterial agents use organic antibacterial agents with certain toxicity, such as vanillin or ethyl vanillin compounds. Under such circumstances, there is an urgent need in the field of food safety for an antibacterial agent having a broad-spectrum antibacterial effect, which is safe and nontoxic.

The double arginine is one of the smallest biological self-assembly materials, has the following structural formula, and is formed by dehydrating and condensing two arginines, and one end of the double arginine isIs amino-NH₂One end is carboxyl-COOH, existing in a zwitterionic form at isoelectric point, i.e. -NH₃ ⁺One end is-COO^-. The double arginine can be self-assembled in a plurality of solvents, such as methanol, chloroform, water and the like, is a short peptide which is widely researched, and is a biological self-assembly material with wide application prospect because the double arginine is composed of amino acid.

Plasma (plasma) refers to a highly ionized gas, which is ionized when exposed to high external energy to generate a charge-neutral system consisting of a large number of charged particle ions, electrons, neutral particle atoms and molecules. The various reactive species in the plasma state can generate different free radicals and functional groups that will change the surface properties of the material, but not the overall properties. The energy of most particles in the low-temperature plasma is slightly higher than the chemical bond energy in the polymer, and the low-temperature plasma has enough energy to cause various chemical bonds on the surface of the polymer to be broken or recombined after being accelerated by an electric field, so that the surface of the molecular material can be modified. For example, plasma treatment of some nanofiber membranes can alter the surface properties of the membrane and increase the solubility of the membrane. If the low-density polyethylene is treated by plasma, the adsorption quantity of nisin can be increased, and the antibacterial and fresh-keeping effects on food can be achieved. Therefore, the surface treatment modification of the double arginine by using the low-temperature plasma is tried to further improve the antibacterial effect of the double arginine.

There are many domestic patent applications for cold plasma. Chinese patent CN107633993B discloses a cold plasma double glow discharge vegetable and fruit seed treatment device; chinese patent CN106381690A discloses a method for preparing a high-strength antibacterial oil-water separation type material by cold plasma pretreatment; chinese patent CN109122816A discloses a preparation method and application of a plasma-treated Majorana Hortensis extract. But no patent is made on the cold plasma treated arginines.

The cold plasma and the double arginine are combined to prepare the novel antibacterial agent with excellent antibacterial effect, and the novel antibacterial agent has better application prospect and market value.

Disclosure of Invention

The invention aims to provide a cold plasma treated biarginine antibacterial agent, a preparation method and application thereof in the field of food safety.

The preparation method of the antibacterial agent comprises the steps of firstly treating the solid powder of the double arginine by using cold plasma to obtain the cold plasma modified double arginine; and adding the arginines into the acetic acid solution, and fully stirring to dissolve the arginines to obtain the arginines acetic acid solution, namely the antibacterial agent.

The cold plasma takes the mixed gas of nitrogen and oxygen as the exciting gas, the flow rate is 100sccm, the treatment time of the cold plasma is 3-5min, and the treatment power is 300-.

The volume ratio of the nitrogen to the oxygen is 1: 1.

In the acetic acid bisarginine solution: the concentration of the double arginine is 2-10mg/mL, and the volume percentage concentration of the acetic acid is 1% -5%.

The stirring condition is that magnetic stirring is carried out for 30min under the condition of the rotating speed of 25 r/min.

The energy of most particles in the low-temperature plasma is slightly higher than the chemical bond energy in the polymer, and the low-temperature plasma has enough energy to cause various chemical bonds on the surface of the polymer to be broken or recombined after being accelerated by an electric field, so that the surface of the molecular material can be modified. The invention utilizes the advantage of high energy of cold plasma to carry out surface modification on the double arginine, thereby further improving the antibacterial effect of the double arginine. The double arginine antibacterial agent treated by the cold plasma can realize the effects of safety, no toxicity, low dose, high efficiency (economy), broad-spectrum antibacterial property and the like.

Drawings

FIG. 1 the antimicrobial effect of a cold plasma treated bis-arginine antimicrobial on Salmonella.

Figure 2 antimicrobial effect of cold plasma treated bis-arginine antimicrobial in chicken broth against salmonella.

Figure 3 antimicrobial effect of cold plasma treated biarginine antimicrobial on Methicillin Resistant Staphylococcus Aureus (MRSA).

Figure 4 antimicrobial effect of cold plasma treated biarginine antimicrobial against methicillin-resistant staphylococcus aureus (MRSA) in pork broth.

N.d. indicates no detection.

Detailed Description

The following examples illustrate specific embodiments of the present invention, but the scope of the present invention is not limited thereto.