阅读说明：本技术 一种罗望子种仁蛋白的提取方法 (A kind of extracting method of tamarind protein ) 是由 张娜 宋子波 艾连中 于 2019-09-16 设计创作，主要内容包括：一种罗望子种仁蛋白的提取方法,属于植物蛋白提取技术领域,食品加工技术领域。为了解决罗望子种仁中由于多糖存在粘稠度高导致蛋白质提取率低的问题,本发明提供了一种罗望子种仁蛋白的提取方法,将提取多糖后的罗望子种仁粉碎获得种仁粉；经柠檬酸进一步去除多糖残留后,离心收集沉淀；将沉淀与缓冲溶液Tris-HCl混合,调节pH为11.0～13.0,获得的混合物料经加热后进行超声波处理；在pH4.0-5.0条件下离心收集沉淀物,然后经分步提取获得种仁碱溶性球蛋白、种仁清蛋白、种仁盐溶性球蛋白、种仁醇溶蛋白和种仁谷蛋白。本发明可用于规模化的生产罗望子种仁蛋白。(A kind of extracting method of tamarind protein, belongs to vegetable protein extractive technique field, food processing technology field.In order to solve the problems, such as that since there are viscosity height to cause extraction rate of protein low for polysaccharide in tamarind kernel, the present invention provides a kind of extracting methods of tamarind protein, and the tamarind kernel after extraction polysaccharide is crushed and obtains kernel powder；After the further Polysaccharide removing residual of citric acid, precipitating is collected by centrifugation；Precipitating is mixed with buffer solution Tris-HCl, adjusting pH is 11.0~13.0, carries out ultrasonication after the mixed material of acquisition is heated；Sediment is collected by centrifugation under the conditions of pH4.0-5.0, then obtains kernel alkali solubility globulin, kernel albumin, kernel salt dissolubility globulin, kernel alcohol soluble protein and kernel glutelin through step by step arithmetic.The present invention can be used for the production tamarind protein of scale.)

1. a kind of extracting method of tamarind protein, which is characterized in that successively carry out as steps described below:

Step 1: the tamarind kernel after extraction polysaccharide is crushed, sieving, obtains kernel powder；

Step 2: by kernel powder and pH3.6 citric acid solution according to 1g:(30-50) mL ratio mixing, 800-1000 turn/min 30-50min is stirred, centrifugation is precipitated；

Step 3: the precipitating obtained in step 2 is mixed with Tris-HCl buffer solution, the precipitating and buffer solution Tris- The ratio of HCl mixing is 1g:(20-40) mL, adjusting pH value is 11.0~13.0, obtains mixed material；

Step 4: mixed material heats 40-90min under the conditions of 60~70 DEG C；

Step 5: and then mixed material is subjected to ultrasonication, the treatment conditions of the ultrasonic wave are as follows: 200~250W of power, 40~60min of time；

Step 6: the material after ultrasonication in step 5 is centrifuged, the precipitating and supernatant of acquisition adjust supernatant PH4.0~5.0 are stood, and obtain kernel alkali solubility globulin after the sediment being collected after centrifugation is freeze-dried；

Step 7: after the precipitating washing that material centrifugation in step 6 is obtained, according to 1g:(20-40) solid-liquid ratio of mL and water it is mixed It closing, 800-1000 turns/min stirring 60-90min, is then centrifuged for, and the supernatant of acquisition is freeze-dried to obtain kernel albumin, The precipitating of acquisition is stand-by；

Step 8: then, the precipitating that step 7 is obtained, according to 1g:(20-40) solid-liquid ratio of mL mixes with NaCl solution, 800-1000 turns/min stirring 60-90min, and it is kernel salt dissolubility globulin that centrifugation, which obtains supernatant, and the precipitating of acquisition is stand-by；

Step 9: then, the precipitating that step 8 is obtained, according to 1g:(20-40) solid-liquid ratio of mL mixes with ethanol solution, 800-1000 turns/min stirring 60-90min, and it is kernel alcohol soluble protein that centrifugation, which obtains supernatant, and the precipitating of acquisition is stand-by；

Step 10: then, the precipitating that step 9 is obtained, according to 1g:(20-40) solid-liquid ratio of mL mixes with NaOH solution, 800-1000 turns/min stirring 60-90min, and it is kernel glutelin that centrifugation, which obtains supernatant,.

2. extracting method according to claim 1, which is characterized in that the tamarind kind after extraction polysaccharide described in step 1 Polysaccharide residual quantity is 2.0-3.5g/100g in benevolence.

3. extracting method according to claim 1, which is characterized in that the mesh number of sieving described in step 1 is 80-120 Mesh.

4. extracting method according to claim 1, which is characterized in that polysaccharide residual quantity is in precipitating described in step 2 0.5-1.5g/100g。

5. extracting method according to claim 1, which is characterized in that precipitating described in step 3 and buffer solution Tris- The ratio of HCl is 1g:40mL.

6. extracting method according to claim 1, which is characterized in that the treatment conditions of ultrasonic wave described in step 5 are function Rate is 200W, time 60min.

7. extracting method according to claim 1, which is characterized in that pH described in step 6 is 4.6.

8. extracting method according to claim 1, which is characterized in that stood described in step 6, time 30-60min.

9. extracting method according to claim 1, which is characterized in that precipitating the ratio mixed with water described in step 7 is 1g:40mL.

10. extracting method according to claim 1, which is characterized in that NaCl solution concentration described in step 8 is 5% (quality), precipitating the ratio mixed with NaCl solution is 1g:40mL.

11. extracting method according to claim 1, which is characterized in that the concentration of ethanol solution described in step 9 is 70% (volume), precipitating the ratio mixed with ethanol solution is 1g:40mL.

12. extracting method according to claim 1, which is characterized in that the concentration of NaOH solution described in step 10 is 0.25% (quality), precipitating the ratio mixed with NaOH solution is 1g:40mL.

Technical field

The invention belongs to vegetable protein extractive technique field, food processing technology fields, and in particular to a kind of tamarind kind The extracting method of benevolence albumen.

Background technique

The method that the current relatively conventional protein in tamarind kernel extracts is alkali extraction-acid precipitation, but is not had Specific protein settles isoelectric point, and recovery rate is not high；Other uncommon extracting methods make egg by sodium chloride solution White matter is separated by Micellization process wherein, tamarind kernel powder and the NaCl solution of degreasing is sufficiently mixed, then pass through salt Analysis dialysis finally obtains protein concentrate, but protein extracting ratio is not high, and without tool that clear tamarind protein is included Body protein type and content.After factory extracts tamarind kernel polysaccharide at present, there are also polysaccharide residuals to lead to the sticky influence kind of solution The extraction of benevolence albumen causes valuable protein resource in tamarind kernel not utilize well.

Summary of the invention

In order to solve the problems, such as that extraction rate of protein is low in tamarind kernel, and clear tamarind protein type and contain Amount, the present invention provides a kind of extracting methods of tamarind protein, include the following steps:

Step 1: the tamarind kernel after extraction polysaccharide is crushed, sieving, obtains kernel powder；

Step 2: by kernel powder and pH3.6 citric acid solution according to 1g:(30-50) mL ratio mixing, 800-1000 Turn/min stirring 30-50min, centrifugation is precipitated；

Step 3: the precipitating obtained in step 2 is mixed with Tris-HCl buffer solution, the precipitating and buffer solution The ratio of Tris-HCl mixing is 1g:(20-40) mL, adjusting pH value is 11.0~13.0, obtains mixed material；

Step 4: mixed material heats 40-90min under the conditions of 60~70 DEG C；

Step 5: and then mixed material is subjected to ultrasonication, the treatment conditions of the ultrasonic wave are as follows: power 200~ 250W, 40~60min of time；

Step 6: the material after ultrasonication in step 5 is centrifuged, the precipitating and supernatant of acquisition, by supernatant tune PH4.0~5.0 are saved, are stood, obtain kernel alkali solubility globulin after the sediment being collected after centrifugation is freeze-dried；

Step 7: after the precipitating washing that material centrifugation in step 6 is obtained, according to 1g:(20-40) solid-liquid ratio of mL with Water mixing, 800-1000 turn/min stirring 60-90min, are then centrifuged for, the supernatant of acquisition is freeze-dried to obtain the clear egg of kernel White, the precipitating of acquisition is stand-by；

Step 8: then, the precipitating that step 7 is obtained, according to 1g:(20-40) solid-liquid ratio of mL and NaCl solution it is mixed Close, 800-1000 turns/min stirs 60-90min, and it is kernel salt dissolubility globulin that centrifugation, which obtains supernatant, the precipitating of acquisition to With；

Step 9: then, the precipitating that step 8 is obtained, according to 1g:(20-40) solid-liquid ratio of mL and ethanol solution it is mixed It closes, 800-1000 turns/min stirring 60-90min, and it is kernel alcohol soluble protein that centrifugation, which obtains supernatant, and the precipitating of acquisition is stand-by；

Step 10: then, the precipitating that step 9 is obtained, according to 1g:(20-40) solid-liquid ratio of mL and NaOH solution it is mixed It closes, 800-1000 turns/min stirring 60-90min, and it is kernel glutelin that centrifugation, which obtains supernatant,.

It further limits, polysaccharide residual quantity is 2.0- in the tamarind kernel after extraction polysaccharide described in step 1 3.5g/100g。

It further limits, the mesh number of sieving described in step 1 is 80-120 mesh.

It further limits, polysaccharide residual quantity is 0.5-1.5g/100g in precipitating described in step 2.

It further limits, the ratio of precipitating described in step 3 and buffer solution Tris-HCl are 1g:40mL.

It further limits, the reagent that adjusting pH value described in step 3 uses is food grade sodium hydroxide.

It further limits, the treatment conditions of ultrasonic wave described in step 5 are that power is 200W, time 60min.

It further limits, the reagent that adjusting pH value described in step 6 uses is food grade hydrochloric acid.

It further limits, pH described in step 6 is 4.6.

It further limits, is stood described in step 6, time 30-60min.

It further limits, it is 1g:40mL that the ratio mixed with water is precipitated described in step 7.

It further limits, NaCl solution concentration described in step 8 is 5% (quality), what precipitating was mixed with NaCl solution Ratio is 1g:40mL.

It further limits, the concentration of ethanol solution described in step 9 is 70% (volume), and precipitating is mixed with ethanol solution The ratio of conjunction is 1g:40mL.

It further limits, the concentration of NaOH solution described in step 10 is 0.25% (quality), precipitating and NaOH solution Mixed ratio is 1g:40mL.

Beneficial effect

The present invention provides a kind of tamarind protein extraction processes, have the advantage that compared with prior art

1. the present invention solves the problems, such as that tamarind seed polysaccharide solution is sticky, tamarind protein extraction process can be significant The dissolution rate of protein is improved, to improve the recovery rate of protein.

2. present invention determine that the isoelectric point of tamarind protein is 4.0-5.0.

3. handling by using ultrasonic wave kernel powder mixed liquor, there is cavitation to kernel powder, it can be effective Promotion protein separation, to significantly improve the separation rate of polysaccharide and albumen, effectively raise purity of protein, energy The sliminess for enough reducing protein fills discarded protein to further improve protein extracting ratio The processing and utilization divided.

4. the present invention goes back while the protein classes and content of tamarind kernel has been determined.

It is the production tamarind protein of high-volume scale 5. the present invention provides broad space for the exploitation of tamarind Precondition is provided.

Specific embodiment

Raw material, reagent and instrument and equipment of the present invention can be bought by commercialization approach and be obtained.