阅读说明：本技术 一种具有神经保护及提高记忆的保健品组合物及制备方法 (Health-care product composition with functions of protecting nerves and improving memory and preparation method thereof ) 是由 王迪 孔繁格 胡文继 官月 李玉 刘洋 李兰州 于 2021-08-11 设计创作，主要内容包括：本发明提供一种具有神经保护及提高记忆的保健品组合物,以珊瑚状猴头菌、蜜环菌、牛肝菌及羊肚菌混合提取物为主要原料,以一定比例混合后获得的提取物作为复方保健品,通过协同作用提升记忆力和认知能力,抗氧化及缓解阿尔茨海默病的症状,相对于单独使用一种菌粉相比,具有更好的效果。(The invention provides a health-care product composition with nerve protection and memory improvement, which takes the mixed extracts of Hericium coralloides, Armillaria mellea, bolete and morchella as main raw materials, takes the extracts obtained after mixing in a certain proportion as a compound health-care product, improves the memory and cognitive ability through synergistic action, resists oxidation and relieves the symptoms of Alzheimer disease, and has better effect compared with the single use of one kind of bacterial powder.)

1. A health product composition with nerve protection and memory improvement functions is characterized by being prepared from the following raw materials in parts by weight:

40-65 parts of coralloid hericium erinaceus powder, 20-40 parts of armillaria mellea powder, 5-15 parts of bolete powder and 10-20 parts of morchella powder.

2. The health food composition with neuroprotection and memory enhancement of claim 1, wherein:

50 parts of coralloid hericium erinaceus powder, 25 parts of armillaria mellea powder, 10 parts of bolete powder and 15 parts of morchella esculenta powder.

3. The preparation method of the health-care product composition with the functions of protecting nerves and improving memory according to claim 1 or 2, which is prepared by the following steps:

1) weighing the raw materials in proportion, respectively sieving with a 50-mesh sieve, uniformly mixing, adding water, decocting for 1.5-3 hours, wherein the mass of the added water is 10-40 times of that of the composition every time, and obtaining a decoction;

2) filtering the decoction, concentrating under reduced pressure, and spray drying to obtain powder.

4. The health-care composition with neuroprotection and memory improvement according to claim 1 or 2, which can be made into pharmaceutical preparations such as powder, oral liquid, granule, tablet, capsule, pill, syrup or paste.

5. The health-care composition with neuroprotection and memory improvement as claimed in claim 1 or 2, wherein conventional pharmaceutically acceptable adjuvants can be added.

Technical Field

The invention belongs to the technical field of health-care product processing, particularly relates to a health-care product composition with functions of protecting nerves and improving memory, and also provides a preparation method of the health-care product composition.

Background

Alzheimer's Disease (AD) causes a decline in memory and other cognitive abilities, which accounts for over 60% of dementia cases. Most of the existing marketed drugs aim at a single target point to serve as a brain metabolism improving agent or a neuroprotective agent, but the effect is very little. In recent years, with the progressive research on the pharmacological activity of traditional Chinese medicines, the clinical use of bacterial medicines has a remarkable auxiliary treatment effect on AD patients, and the AD symptom relief and progress show a considerable development trend. Since alzheimer disease cannot be cured at present, prevention becomes the only choice for middle-aged and elderly people, and functional foods related to the alzheimer disease also become the need.

Disclosure of Invention

The invention provides a health-care product composition with functions of protecting nerves and improving memory, which can improve memory and cognitive ability and has obvious antioxidant and anti-aging effects.

The invention provides a health-care product composition with functions of protecting nerves and improving memory, which is prepared from the following raw materials in parts by weight: 40-65 parts of coralloid hericium erinaceus powder, 20-40 parts of armillaria mellea powder, 5-15 parts of bolete powder and 10-20 parts of morchella powder.

Preferably, the health-care product composition with the functions of protecting nerves and improving memory comprises the following raw materials in parts by weight: 50 parts of coralloid hericium erinaceus powder, 25 parts of armillaria mellea powder, 10 parts of bolete powder and 15 parts of morchella esculenta powder.

The invention provides a preparation method of a health-care product composition with functions of protecting nerves and improving memory, which is prepared by the following steps:

(1) weighing the raw materials in proportion, respectively sieving with a 50-mesh sieve, uniformly mixing, adding water, decocting for 1.5-3 hours, wherein the mass of the added water is 10-40 times of that of the composition every time, and obtaining a decoction;

(2) filtering the decoction, concentrating under reduced pressure, and spray drying to obtain powder.

Preferably, the health-care product composition with the functions of protecting nerves and improving memory is prepared by the following steps:

(1) weighing the raw materials in proportion, respectively sieving with a 50-mesh sieve, uniformly mixing, adding water, decocting for 2.5 hours, wherein the mass of the added water is 30 times of that of the composition every time, and obtaining a decoction;

(2) filtering the decoction, concentrating under reduced pressure, and spray drying to obtain powder.

The invention also provides application of the health-care product composition with the functions of protecting nerves and improving memory in preparing preparations for improving memory and cognitive ability and improving senile dementia.

The preparation for improving memory and cognitive ability and senile dementia can be powder, oral liquid, granule, tablet, capsule, pill, syrup or paste.

The preparation for improving memory and cognitive ability and improving senile dementia can be added with pharmaceutically acceptable conventional auxiliary materials.

[ DOSAGE ] 3.0-6.0 g/(60 kg human body);

[ APPLICATION STATUS ] the memory is decreased; neurasthenia; alzheimer's disease.

The pharmacological and medicinal properties of the invention are as follows: the extracts obtained by extracting the four bacterium powders in a certain proportion are used as compound health care products, and the memory and cognitive abilities are improved through synergistic action, so that the effects of resisting oxidation and relieving the symptoms of Alzheimer disease are better compared with the effects of singly using one of the bacterium powders.

The invention has the following beneficial effects:

the compound preparation is prepared according to the compatibility theory of traditional Chinese medicine and the theory of combining traditional Chinese medicine with western medicine, the mixed extract of Hericium coralloides, Armillaria mellea, bolete and morchella esculenta is used as the main raw material, the extract obtained after mixing in a certain proportion is used as the compound health product, the memory and the cognitive ability are improved through the synergistic effect, and the compound health product is antioxidant and can relieve the symptoms of Alzheimer disease.

Detailed Description

The present invention will be further described with reference to the following examples. These examples are merely illustrative and do not limit the scope of the present invention in any way. It will be understood by those skilled in the art that various changes in form and details may be made therein without departing from the spirit and scope of the invention, and that such changes and modifications may be made without departing from the spirit and scope of the invention.

The coralloid hericium erinaceus powder used in the examples and comparative examples was obtained by pulverizing the fruiting body of coralloid hericium erinaceus produced in Changbai mountain with a pulverizer, and was used in the subsequent experiments as a brown-yellow powder.

The Armillaria mellea powder used in the examples and comparative examples is Armillaria mellea mycelium obtained by submerged liquid fermentation, and the Armillaria mellea mycelium obtained by centrifugation, freeze-drying and crushing by a crusher is light yellow powder and is used for subsequent experiments.

The bolete powder used in examples and comparative examples was obtained by pulverizing a bolete fruit body using a pulverizer, and was brown powder for subsequent experiments.

The morchella powder used in the examples and comparative examples was obtained by pulverizing morchella sporocarp with a pulverizer, and was light yellow powder for subsequent experiments.

Example 1

The health-care product composition with the functions of protecting nerves and improving memory comprises the following raw material medicaments:

50 parts of coralloid hericium erinaceus powder, 25 parts of armillaria mellea powder, 10 parts of bolete powder and 15 parts of morchella esculenta powder.

The preparation method of the composition comprises the following steps:

(1) drying Hericium coralloides fruiting body, Boletus edulis fruiting body, Morchella esculenta fruiting body and Armillaria mellea mycelium, pulverizing with a pulverizer, and sieving with 50 mesh sieve to obtain each bacteria powder;

(2) respectively taking 50 parts of coralline hericium erinaceus powder, 25 parts of armillaria mellea powder, 10 parts of bolete powder and 15 parts of morel powder, and uniformly mixing to obtain the health-care product composition with the functions of protecting nerves and improving memory in the embodiment 1.

Example 2

The health-care product composition with the functions of protecting nerves and improving memory comprises the following raw material medicaments:

40 parts of coralloid hericium erinaceus powder, 30 parts of armillaria mellea powder, 10 parts of bolete powder and 20 parts of morchella esculenta powder.

The preparation method of the composition comprises the following steps:

(1) drying Hericium coralloides fruiting body, Boletus edulis fruiting body, Morchella esculenta fruiting body and Armillaria mellea mycelium, pulverizing with a pulverizer, and sieving with 50 mesh sieve to obtain each bacteria powder;

(2) respectively taking 40 parts of coralline hericium erinaceus powder, 30 parts of armillaria mellea powder, 10 parts of boletus powder and 20 parts of morchella powder, adding deionized water according to the liquid-material ratio of 20:1, and decocting for 3 hours to obtain decoction;

(3) the decoction was filtered, concentrated under reduced pressure, and spray-dried into powder to obtain the neuroprotective and memory-improving nutraceutical composition of example 2, which was prepared into various dosage forms such as granules, tablets, capsules, powders, pills, syrups, or ointments according to the conventional food preparation process for the following efficacy experiments.

Example 3

The health-care product composition with the functions of protecting nerves and improving memory comprises the following raw material medicaments:

50 parts of coralloid hericium erinaceus powder, 20 parts of armillaria mellea powder, 10 parts of bolete powder and 20 parts of morchella esculenta powder.

The preparation method of the composition comprises the following steps:

(1) drying Hericium coralloides fruiting body, Boletus edulis fruiting body, Morchella esculenta fruiting body and Armillaria mellea mycelium, pulverizing with a pulverizer, and sieving with 50 mesh sieve to obtain each bacteria powder;

(2) taking 50 parts of coralline hericium erinaceus powder, 20 parts of armillaria mellea powder, 10 parts of boletus powder and 20 parts of morchella powder respectively, adding deionized water according to a liquid-material ratio of 25:1, and decocting for 2 hours to obtain decoction;

(3) the decoction was filtered, concentrated under reduced pressure, and spray-dried to obtain a powder to obtain the neuroprotective and memory enhancing nutraceutical composition of example 3, which was prepared into various dosage forms such as granules, tablets, capsules, powders, pills, syrups or ointments according to conventional food preparation methods for the following efficacy experiments.

Example 4

The health-care product composition with the functions of protecting nerves and improving memory comprises the following raw material medicaments:

60 parts of coralloid hericium erinaceus powder, 20 parts of armillaria mellea powder, 5 parts of bolete powder and 15 parts of morchella esculenta powder.

The preparation method of the composition comprises the following steps:

(1) drying Hericium coralloides fruiting body, Boletus edulis fruiting body, Morchella esculenta fruiting body and Armillaria mellea mycelium, pulverizing with a pulverizer, and sieving with 50 mesh sieve to obtain each bacteria powder;

(2) respectively taking 60 parts of coralline hericium erinaceus powder, 20 parts of armillaria mellea powder, 5 parts of boletus powder and 15 parts of morchella powder, adding deionized water according to a liquid-material ratio of 30:1, and decocting for 1.5 hours to obtain decoction;

(3) the decoction was filtered, concentrated under reduced pressure, and spray-dried to obtain a powder to obtain the neuroprotective and memory enhancing nutraceutical composition of example 4, which was prepared into various dosage forms such as granules, tablets, capsules, powders, pills, syrups or ointments according to conventional food preparation methods for the following efficacy experiments.

Example 5

The health-care product composition with the functions of protecting nerves and improving memory comprises the following raw material medicaments:

50 parts of coralloid hericium erinaceus powder, 25 parts of armillaria mellea powder, 10 parts of bolete powder and 15 parts of morchella esculenta powder.

The preparation method of the composition comprises the following steps:

(1) drying Hericium coralloides fruiting body, Boletus edulis fruiting body, Morchella esculenta fruiting body and Armillaria mellea mycelium, pulverizing with a pulverizer, and sieving with 50 mesh sieve to obtain each bacteria powder;

(2) taking 50 parts of coralline hericium erinaceus powder, 25 parts of armillaria mellea powder, 10 parts of boletus powder and 15 parts of morchella powder respectively, adding deionized water according to a liquid-material ratio of 30:1, and decocting for 2.5 hours to obtain decoction;

(3) the decoction was filtered, concentrated under reduced pressure, and spray-dried to obtain a powder to obtain the neuroprotective and memory enhancing nutraceutical composition of example 5, which was prepared into various dosage forms such as granules, tablets, capsules, powders, pills, syrups or ointments according to conventional food preparation methods for the following efficacy experiments.

Comparative example 1

The comparative example is coralloid hericium erinaceus powder.

The preparation method of the comparative example comprises the following steps: drying Hericium coralloides fruiting body, pulverizing, and sieving with 50 mesh sieve to obtain Hericium coralloides powder.

Comparative example 2

The comparative example is Armillaria mellea powder.

The preparation method of the comparative example comprises the following steps: freeze drying Armillaria mellea mycelium obtained by fermentation, pulverizing with a pulverizer, and sieving with 50 mesh sieve to obtain Armillaria mellea mycelium powder.

Comparative example 3

The comparative example is boletus powder.

The preparation method of the comparative example comprises the following steps: drying the bolete fruiting body, crushing by a crusher, and sieving by a 50-mesh sieve to obtain bolete powder.

Comparative example 4

The comparative example is morel powder.

The preparation method of the comparative example comprises the following steps: drying the morchella sporocarp, crushing by a crusher, and sieving by a 50-mesh sieve to obtain the morchella powder.

To further verify the effect of the present invention, the following experiment was performed:

test example 1

The test example is to examine the neuroprotective effect of the health product composition having neuroprotection and memory-improving effects.

(1) Test materials

The test drugs are the health-care product composition with the functions of protecting nerves and improving memory obtained in examples 1, 2, 3, 4 and 5 and the coralloid hericium erinaceus powder, armillaria mellea powder, boletus edulis powder and morchella esculenta powder of comparative examples 1, 2, 3 and 4. The mouse cell line used in this experiment was a mouse neuronal cell line (HT 22; BNCC; 337709) from Beiner Chuanglian Biopsis.

(2) Test method

The neuroprotective effect of each fungal nutraceutical composition and fungal monocomponent in the above examples and comparative examples on L-glutamic acid-induced HT22 cell damage was examined. HT22 cells at 5X 10³Cell concentration of cells seeded in 96-well plates, 5% CO₂After observing the cell state, the cells were pretreated at a dose of 25 and 100. mu.g/mL for 3 hours at a constant temperature of 37 ℃ and then modeled with L-glutamic acid (L-Glu) at a final concentration of 25 mM, and the cells were incubated for 24 hours without treatment in the blank group and again administered in the pretreatment mode. Adding 5 mg/mL MTT solution into a 96-well plate in a dark state, incubating for 4 hours in a dark state, dissolving the precipitate with DMSO, detecting the absorbance at 490/540 nm, and analyzing the change of the cell activity.

The survival rate of each group was calculated with the survival rate of the blank group as 100%. Experimental data are expressed as mean ± standard deviation (mean ± s.d.). Statistically significant differences the differences between groups were examined according to the one-way ANOVA analysis of IBM SPSS Statistics 16 software (Dunn's test performed afterwards), P <0.05 considered significant and statistically significant.

(3) Test results

Table 1 shows the effect of the single components of the health product composition with neuroprotection and memory improvement obtained in examples 1, 2, 3, 4 and 5 and the coralloid hericium erinaceus powder, armillaria mellea powder, boletus edulis powder and morchella esculenta powder of comparative examples 1, 2, 3 and 4 on the activity of HT22 cells. Compared with the blank group, after modeling by L-glutamic acid (the final concentration is 25 mM), the cell activity of the control group is reduced to 51.7 percent (P < 0.01), when the administration concentration is 25 mu g/mL, the four bacterial powders of the control group have no obvious neuroprotective effect, when the dosage is increased to 100 mu g/mL, except the bolete powder, the survival rate of cells is remarkably improved by coralloid Hericium, Armillaria mellea and Morchella esculenta (P < 0.05), the neuroprotective effect of example 1 on HT22 cells obtained by mixing the four bacterial powders according to a certain proportion is better than that of a single component which is singly administered, and the bacterial composition obtained by hot water extraction of comparative examples 2, 3, 4 and 5 has obvious neuroprotective effect (P < 0.05) when the administration concentration is 25 mu g/mL, wherein the effect of example 5 is the best, and when the administration concentration is 100 mu g/mL, cell viability was increased to 86.5% (P < 0.01). The health-care product composition with the functions of neuroprotection and memory improvement is proved to have obvious neuroprotective effect on L-glutamic acid induced cell damage by pretreatment and administration, compared with comparative examples 1-4, the composition extract has obvious neuroprotective effect compared with single component bacterial powder, and the effect obtained after the composition is matched is proved to be better.

TABLE 1 neuroprotective effect of a nutraceutical composition with neuroprotection and memory enhancement on HT22 cells

Note: p >0.05 represents no significance, p <0.05 represents significance, and p <0.01 represents very significant.

Test example 2

The influence of the health-care product composition with the functions of protecting nerves and improving memory on the memory improvement and the antioxidant capacity of the mice is detected.

(1) Test materials

Medicine preparation: physiological saline, the health care product composition having neuroprotection and memory improvement obtained in examples 1, 2, 3, 4 and 5, and the coralloid hericium erinaceus powder, armillaria mellea powder, boletus edulis powder and morchella esculenta powder of comparative examples 1, 2, 3 and 4.

Animals: 60 male SPF grade C57BL/6 mice (8 weeks old, 18-22 g in weight) were purchased from the vinca biologies institute, license number: SCXK (Ji) -2011-. All mice were housed in an environment with a temperature of 23 + -1 deg.C and a humidity of about 55%. The lighting time is kept for 12 hours, natural light is uniformly radiated, 6 mice/cage can freely take food and water.

The kit comprises: the biochemical index detection comprises ELISA kits of central cholinergic related indexes such as acetylcholine (ACh), acetylcholinesterase (AChE) and choline acetyltransferase (ChAT) and ELISA kits of oxidative stress related indexes such as ROS and superoxide dismutase (SOD), wherein the kits are all purchased from Jiangsu Kort biotechnology limited company, and all the kit detection methods are operated according to instructions provided in a kit.

(2) Test method

Grouping and administration of mice: selecting 60 SPF grade healthy C57BL/6 male mice, randomly dividing into 10 groups of 6 mice, wherein the groups are as follows: 10 groups were selected for the blank control group, the example 1 group, the example 2 group, the example 3 group, the example 4 group, the example 5 group, the comparative example 1 group (coralloid Hericium powder group), the comparative example 2 group (Armillaria mellea powder group), the comparative example 3 group (Boletus powder group), and the comparative example 4 group (Morchella powder group). Each group was dissolved with distilled water to the appropriate drug concentration. The administration is carried out by gavage at 9:00 morning every day, the administration dose is 10 mg/kg, the gavage volume is 0.1mL/20g of body weight, and the gavage of a blank control group is carried out by gavage with normal saline for 3 weeks continuously.

Animal behavioral testing: the cognitive and memory improving effects of the health care product composition with the functions of neuroprotection and memory improvement on mice are investigated through the Morris water maze.

The water maze consists of a stainless steel pool (diameter is 120 cm, height is 50 cm) and an immersed escape platform (diameter is 10 cm) which is 1cm below the water surface. Running water is injected into the water maze to enable the water maze to submerge the escape platform by about 1cm, titanium dioxide powder is added into the water, the water is uniformly stirred to be white and opaque, and the water temperature is kept at about 25 ℃. The water maze was equally divided into 4 1/4 circles, designated as four quadrants, by the water maze handling system. The escape platform is placed in the second quadrant, and the mouse is thrown into the water from the same position every time. Each experiment ensures that the environment is quiet. Space learning tests were performed for 4 consecutive days and the time required for the mouse to find the hidden platform was recorded as the escape latency. The experimental time is set to be 2min, and mice which do not find the platform within the specified time are artificially guided to the escape platform. The time at day 5 when the mice found the platform was recorded by the water maze monitoring system.

After the last behavioral test, mice were fasted for 12 hours. Blood was sampled from the tail vein, allowed to stand at room temperature for 30min, centrifuged at 3500rpm for 5 min, and after centrifugation twice, the supernatant was collected and stored in a refrigerator at-80 ℃. Mice were then euthanized by intraperitoneal injection of sodium pentobarbital (100 mg/kg). Brain tissue was collected rapidly.

Measuring the central cholinergic related index in the mouse hippocampus: the collected hippocampal tissues were homogenized with physiological saline and the protein concentration was detected by BCA kit (Millipore). And (4) testing according to the instruction provided by the kit, and detecting the content of AChE, Ach and ChAT in the hippocampus of the mouse.

Determination of relevant indexes of oxidative stress in mouse serum: and testing the collected mouse serum according to the instruction provided by the kit, and detecting the contents of SOD and ROS in the mouse serum.

The data processing method comprises the following steps: experimental data are expressed as mean ± standard deviation (mean ± s.d.). Statistically significant differences the differences between groups were examined according to the one-way ANOVA analysis of IBM SPSS Statistics 16 software (Dunn's test performed afterwards), P <0.05 considered significant and statistically significant.

(3) Results of the experiment

Morris water maze test results: the experimental results of the platform finding time, namely the escape latency period, of each group of mice are shown in table 2, the example 1 is the mixed bacterial powder of four bacteria, namely coralloid Hericium erinaceus, Armillaria mellea, boletus and morchella, the platform finding time (P < 0.05) of the mice can be obviously shortened after 3 weeks of administration, the experimental results are superior to the single-component administration, namely comparative examples 2-4 (P > 0.05), and the effect of the experimental results is similar to that of the comparative example 1, namely the effect of the coralloid Hericium erinaceus powder which is independently administered. The health-care product composition with the functions of protecting nerves and improving memory can remarkably shorten the time for a mouse to search for a platform (P < 0.05), and the health-care product composition with the functions of protecting the nerves and improving memory can remarkably shorten the time for the mouse to search for the platform and improve the space memory of the mouse in the examples 3 and 5, wherein the effect of the example 5 is most obvious, and the time is shortened by 39.6 percent (P < 0.01) compared with that of a blank control group of mice. This demonstrates the significant improvement in cognitive and memory in mice administered the compositions of examples 1-5. In addition, when the examples are compared with the comparative examples transversely, the results show that the effects of the examples on shortening the search time of mice due to the synergistic effect are more obvious and have universal significance (P <0.05 and P < 0.01), and the effects of the comparative examples are mostly not significant (P > 0.05).

TABLE 2 Effect of the combination of neuroprotective and memory enhancing nutraceuticals on the Water maze test in mice

Note: data are presented as mean ± standard deviation. (n = 6/group). P >0.05 represents no significance, P <0.05 represents significance, and P <0.01 represents extreme significance.

The determination result of the central cholinergic related index is as follows: acetylcholine (Ach), which is associated with learning, memory and cognitive abilities, is an important neurotransmitter in the brain, reflecting the state of cholinergic neurons. The neurotransmitter Ach can act on a plurality of choline receptors and is also an excitatory neurotransmitter of a plurality of motor nerves, and the increase of the content of the neurotransmitter is related to the improvement of the Alzheimer disease. Both AchE as Ach hydrolase and ChAT as Ach synthetase can be used to modulate Ach metabolism.

The experimental results are shown in table 3, compared with the blank control group, after the mice are subjected to intragastric administration for 3 weeks, only the Hericium coralloides administered to the single bacterial powder of comparative examples 1-4 significantly reduced the AchE level (P < 0.05), the Armillaria mellea, bolete and morchella do not significantly change the AchE level in the hippocampus of the mice (P > 0.05), and the AchE levels (P < 0.01) are significantly reduced in examples 2-5 obtained by hot water extraction of a mixture of the four bacterial substances mixed in a certain ratio. Examples 1-5 significantly increased the Ach content in the hippocampus of mice (P < 0.05) compared to the blank control, with example 5 being the most effective, the content being 1.23 times that of the blank control, whereas in the comparative example, administration of a single powder intragastrically did not significantly change the Ach content in the hippocampus of mice (P > 0.05). For ChAT, example 5 significantly increased ChAT levels in the mouse hippocampus (P < 0.05) compared to the placebo group, while examples 1-4 all significantly increased ChAT levels (P < 0.01), with example 3 being the most effective. Comparative examples 1 and 3 show that single powder administration of Armillaria mellea and Morchella esculenta also significantly increases ChAT level (P < 0.05) in mouse hippocampus, but the effect is not significant in the examples. According to the results of AChE, Ach and ChAT experiments, the health-care product composition with nerve protection and memory improvement can achieve the nerve protection effect by improving the central cholinergic related index level, wherein the Ach and ChAT levels can be obviously changed in the example 1 prepared by mixing the four bacterial powders according to a certain proportion. The health-care product composition with the functions of protecting nerves and improving memory, which is obtained after hot water extraction, shows better effect. Although the comparative examples have the same effect, the effect of the single action is not obvious and has no significance because the action routes of different bacteria are different.

TABLE 3 influence of the health composition with neuroprotection and memory enhancement on cholinergic related indices in the mouse center

Note: in comparison with the blank control group,^*P<0.05，^**P<0.01。

the determination result of the oxidative stress related indexes comprises the following steps: oxidative stress is the basis of an important hypothesis of the pathophysiology of many diseases, including neurodegenerative diseases. SOD, which is a natural enemy of oxygen free radicals, is a scavenger of organisms, can remove harmful substances generated in the organisms, has an anti-aging effect, can prevent the generation of oxygen free radicals, and avoids the damage of the oxygen free radicals to cells, thereby having a repairing effect on damaged cells. As shown in table 4, compared with the blank control group, example 2 can significantly increase the SOD content in the serum of the mouse (P < 0.05), and examples 3, 4 and 5 can significantly increase the SOD content in the serum of the mouse (P < 0.01). In addition, the SOD content in the mouse serum is also improved by the example 1 and the comparative example, but the effect is not obvious. In addition, examples 2, 4 and 5 and comparative example 3 can significantly scavenge ROS in mouse serum, on the basis of which the scavenging effect of examples 2 and 3 on ROS is very significant (p < 0.01), wherein example 2 ROS levels are reduced by 33.6% (p < 0.01) compared to the blank control. In conclusion, the health-care product composition with the functions of protecting nerves and improving memory can improve the oxidative stress state of mice. Compared with the single-component bacterial powder of the comparative examples 1 to 4, the four bacterial powders compounded according to a certain proportion show better antioxidant effect after being leached by hot water.

TABLE 4 Effect of neuroprotective and memory-enhancing compositions on the content of oxidative stress indicators in mouse serum

Note: in comparison with the blank control group,^*P<0.05，^**P<0.01

in vitro cell experiments show that the health-care product composition with neuroprotection and memory improvement obtained after hot water extraction has better neuroprotection effect than single bacterial powder, and the same experimental results are verified in animal experiments. The above experimental results show that, compared with the blank control group and the comparative example, namely the single-component bacterial powder, the examples show good memory and cognitive abilities improvement, and simultaneously have obvious antioxidant and anti-aging effects. Example 1 is an untreated mixed bacterial powder showing a certain effect of improving memory and preventing senile dementia, but examples 2 to 5 obtained after hot water extraction show more excellent effects, and in conclusion, the composition of the present invention can be used as a health care composition for improving cognitive and memory abilities and preventing possible senile dementia.