阅读说明：本技术 一种夏枯草总黄酮的制备方法 (A kind of preparation method of Prunella vulgaris general flavone ) 是由 杨成东 于 2018-05-25 设计创作，主要内容包括：本发明涉及一种夏枯草总黄酮的制备方法,具体涉及一种采用匀浆-超声法提取夏枯草总黄酮的方法。其处理步骤包括：将夏枯草阴干,粉碎,加入原料1~2倍的50~60%的乙醇,置于匀浆机中混合匀浆；匀浆后,将物料置于超声波装置中,加入原料3~5倍的50~60%的乙醇溶液,进行超声萃取90~120min,减压浓缩,回收乙醇,得浓缩液；将所得浓缩液经80~100目聚酰胺树脂上柱、洗脱,收集洗脱液,浓缩、干燥,获得夏枯草总黄酮。本发明具有快速、高效、溶剂使用量少等优点,且重现性好,树脂可重复使用。(The present invention relates to a kind of preparation methods of Prunella vulgaris general flavone, and in particular to a method of Prunella vulgaris general flavone is extracted using homogenate-ultrasonic method.Its processing step includes: that Prunella vulgaris is dried in the shade, and is crushed, and 1 ~ 2 times of raw material of 50 ~ 60% ethyl alcohol is added, and is placed in refiner and mixes homogenate；After homogenate, material is placed in ultrasonic unit, 3 ~ 5 times of raw material of 50 ~ 60% ethanol solution is added, carries out 90 ~ 120min of ultrasonic extraction, is concentrated under reduced pressure, ethyl alcohol is recycled, obtains concentrate；By gained concentrate through 80 ~ 100 mesh polyamide upper props, elution, eluent, concentration, dry, acquisition Prunella vulgaris general flavone are collected.The present invention has many advantages, such as that quick, efficient, solvent usage amount is few, and favorable reproducibility, resin are reusable.)

1. a kind of preparation method of Prunella vulgaris general flavone, it is characterised in that:

A. Prunella vulgaris is dried in the shade, is crushed, 1 ~ 2 times of raw material of 50 ~ 60% ethyl alcohol is added, be placed in refiner and mix homogenate 15min；

B. after being homogenized, material is placed in ultrasonic unit, 3 ~ 5 times of raw material of 50 ~ 60% ethanol solution is added, carries out ultrasound 90 ~ 120min is extracted, is concentrated under reduced pressure, ethyl alcohol is recycled, obtains concentrate；

C. by the polyamide resin column of 80 ~ 100 mesh on gained concentrate, 60 ~ 70% elution through 4 ~ 6 times of resin volumes is collected Eluent, concentration, dry, acquisition Prunella vulgaris general flavone.

2. a kind of preparation method of Prunella vulgaris general flavone according to claim 1, which is characterized in that described in step a Refiner power is 200W, and homogenate revolving speed is 10000 ~ 20000r/min.

3. a kind of preparation method of Prunella vulgaris general flavone according to claim 1, which is characterized in that described in step b The condition of ultrasonic extraction are as follows: frequency 40KHz, 50 ~ 70 DEG C of ultrasonic temperature.

4. a kind of preparation method of Prunella vulgaris general flavone according to claim 1, which is characterized in that described in step c The mass ratio of amount of resin and raw material is 1:1.

Technical field

The present invention relates to a kind of preparation methods of Prunella vulgaris general flavone, belong to traditional Chinese medicine extyaction field.

Background technique

Prunella vulgaris, also known as iron color grass, big headdress flower, stick column cap flower, sheep intestines dish, Prunella vulgaris Prunella vulgaris hammer are careless, June is dry, stick Headpin etc..For Lamiales Lamiaceae plant, usually partial desiccation fruit ear is taken to be used as medicine in summer, but common in TaiWan, China market Herb uses.Prunella vulgaris beverage all on sale is spread in general herbal tea.Mainly it is grown on sparse woods, deserted mountain, ridge and roadside, florescence 4-6 Month, the fruiting period 7-10 month.Due to i.e. withered after this careless Summer Solstice, therefore there is this name.Prunella vulgaris is cold in nature, sweet in flavor, pungent, slight bitter, has and lets out liver clearly The effect of fire, mass dissipating and swelling eliminating, clearing heat and detoxicating, expelling phlegm and arresting coughing, cooling blood and hemostasis, is suitable for scrofula, goitre, acute mastitis, head Dizzy dizzy, facial paralysis, arthralgia and myalgia, pulmonary tuberculosis, metrorrhagia, leukorrhagia, Fiedeler's disease type hepatitis and bacillary dysentery etc.. Modern pharmacological studies have shown that Prunella vulgaris reduces blood pressure, and can expand blood vessel, contained rutin has anti-inflammatory effect, and Vasopermeability can be reduced, brittleness is reduced, reduces liver rouge.There are also the effects for resisting cancer cell for Prunella vulgaris.

Flavone compound refers to that basic parent nucleus is the series compound of 2- phenyl chromone, is widely present in plant kingdom, Many medium-height grass the effective elements of the medicines, it is many kinds of, have multiple biological activities, except with antibacterial, anti-inflammatory, it is disease-resistant become, decompression, Outside the effects of clearing heat and detoxicating, calm, diuresis, anti-oxidant, anticancer, anti-cancer, inhibit in terms of also have remarkable result, be A kind of important natural organic oxidation-resistant agent with development prospect.The research trend of flavone compound is medicinal in its in recent years The exploitation of value is more related to the application study of extraction process, the assay of flavone compound and preparation preparation.

Solvent extraction and microwave cooperating extraction method are mostly used to the extraction of Prunella vulgaris general flavone at present.In solvent extraction With ethanol extraction method efficiency highest, but product composition is complicated, time-consuming；The microwave that microwave method energy conservation, time shorten dramatically, but reveals It is more likely to cause environmental pollution.

Homogenate, which is extracted, refers to that tissue carries out tissue homogenate or defibrination by addition extractant, with extract in tissue effectively at A kind of extracting method divided.Effective ingredients in plant is extracted using homogenate method, directly material can be placed in refiner, with extraction Solvent mixes homogenate in homogenization apparatus, and material is torn and crushed by the effect of mechanical and aquashear, keep material broken and The extraction of effective component is synchronous to be carried out, and achievees the purpose that, reinforcing quick to effective ingredients in plant is extracted.

In conjunction with the cavitation effect and mechanical effect of ultrasonic wave, so that the effective component in cell be made to be released directly It contacts and is dissolved wherein with solvent, to improve the recovery rate of effective component.

Summary of the invention

The present invention be directed to the deficiencies in the prior art, it is desirable to provide a kind of high efficiency and it is selective, easy to operate, yield is high, secondary Product is few, can realization of industrialization preparation Prunella vulgaris general flavone method.

Technical solution of the present invention is carried out according to the following steps:

A. Prunella vulgaris is dried in the shade, is crushed, 1 ~ 2 times of raw material of 50 ~ 60% ethyl alcohol is added, be placed in refiner and mix homogenate 15min；

B. after being homogenized, material is placed in ultrasonic unit, 3 ~ 5 times of raw material of 50 ~ 60% ethanol solution is added, carries out ultrasound 90 ~ 120min is extracted, is concentrated under reduced pressure, ethyl alcohol is recycled, obtains concentrate；

C. by the polyamide resin column of 80 ~ 100 mesh on gained concentrate, 60 ~ 70% elution through 4 ~ 6 times of resin volumes is collected Eluent, concentration, dry, acquisition Prunella vulgaris general flavone.

Refiner power described in step a is 200W, and homogenate revolving speed is 10000 ~ 20000r/min.

The condition of ultrasonic extraction described in step b are as follows: frequency 40KHz, 50 ~ 70 DEG C of ultrasonic temperature.

The mass ratio of amount of resin described in step c and raw material is 1:1.

The present invention has the advantages that

1. the present invention handles raw material using plant homogenates extraction method, realizes crushing, extracts step completion, simultaneously because eliminating Heating, so as to effectively avoid the destruction of general flavone ingredient.

2. the present invention uses homogenate-ultrasonic extraction Prunella vulgaris general flavone, extraction low, quick, efficient with production cost The high advantage of rate.

The present invention is further illustrated below in conjunction with specific embodiment, but the scope of protection of present invention is not limited to In following embodiments.

Specific embodiment: