阅读说明：本技术 一种具有调控毛发生长的多聚磷酸盐及其制备方法 (Polyphosphate capable of regulating and controlling hair growth and preparation method thereof ) 是由 吴庆辉 于 2021-09-27 设计创作，主要内容包括：本发明提供了一种具有调控毛发生长的多聚磷酸盐及其制备方法,包括磷酸二氢盐：其组分包括磷酸二氢钠、磷酸二氢钾、磷酸二氢钙中的一种或几种,磷酸二氢盐组分总体占比为20-100％；磷酸氢二盐：其组分包括磷酸氢二钠、磷酸氢二钾、磷酸氢二钙中的一种或几种,磷酸氢二盐组分总体占比为0-80％,制备工艺包括在400-500℃进行预热处理,其次升温值1000-1300℃进行保温处理,最后淬火处理获得产品,依据材料使用性能进行球磨获得不同尺度的样品。本发明克服了现有技术的不足,本发明通过特定的磷酸二氢盐和磷酸二氢钠的比例配合具有调控毛发生长的重要作用,适用于防脱生发产品。(The invention provides a polyphosphate for regulating and controlling hair growth and a preparation method thereof, wherein the polyphosphate comprises dihydric phosphate: the components of the compound comprise one or more of sodium dihydrogen phosphate, potassium dihydrogen phosphate and calcium dihydrogen phosphate, and the total proportion of the components of the dihydrogen phosphate is 20-100%; dibasic acid phosphate: the preparation process comprises the steps of preheating at 400-500 ℃, carrying out heat preservation at 1300 ℃ with the temperature rise value of 1000-1300 ℃, carrying out quenching treatment to obtain a product, and carrying out ball milling according to the service performance of the material to obtain samples with different scales. The invention overcomes the defects of the prior art, has the important function of regulating and controlling the hair growth by the specific proportion of the dihydric phosphate and the sodium dihydrogen phosphate, and is suitable for the hair loss preventing and hair growth promoting product.)

1. A polyphosphate for regulating hair growth, comprising:

dihydrogen phosphate salt: the components of the compound comprise one or more of sodium dihydrogen phosphate, potassium dihydrogen phosphate and calcium dihydrogen phosphate, and the total proportion of the components of the dihydrogen phosphate is 20-100%;

dibasic acid phosphate: the components of the compound comprise one or more of disodium hydrogen phosphate, dipotassium hydrogen phosphate and dicalcium hydrogen phosphate, and the total proportion of the disodium hydrogen phosphate is 0-80%.

2. The polyphosphate salt for controlling hair growth according to claim 1, wherein the polyphosphate salt comprises: preferably, the total proportion of calcium dihydrogen phosphate is 25-35%, and the total proportion of dipotassium hydrogen phosphate is 65-75%.

3. The method of claim 1, comprising the steps of:

firstly, putting dihydric phosphate and/or dibasic phosphate powder into a muffle furnace at 400-500 ℃ for preheating treatment for 1-2 hours;

step two, heating the product in the step one to 1300 ℃ at the temperature of 1000 ℃, and then carrying out constant temperature treatment for 8-12 hours;

step three, quenching the generated article in the step two at 40-60 ℃ to obtain a product, and performing ball milling according to the material use performance to obtain samples with different scales.

4. The method of claim 1, wherein the application of the polyphosphate for regulating hair growth comprises: the polyphosphate is applied to the hair loss prevention and hair growth promotion product.

5. The use method of the hair loss preventing and hair growing product containing polyphosphate as claimed in claim 4, wherein the hair loss preventing and hair growing product containing polyphosphate is uniformly smeared on the surface of hair loss fur and absorbed by massage.

Technical Field

The invention relates to the technical field of hair growth, in particular to a polyphosphate capable of regulating and controlling hair growth and a preparation method thereof.

Background

Hair, which refers to hair growing on the head. The hair can be divided into four parts of hair papilla, hair follicle, hair root and hair shaft from bottom to top. The physiological characteristics and functions of hair depend mainly on the papilla, hair follicle, sebaceous gland, etc. below the scalp epidermis. The hair follicle is the part of hair root in the dermis layer, and consists of an inner hair root sheath, an outer hair root sheath and a hair bulb, wherein the inner hair root sheath is the sheath layer directly adjacent to the hair in the later period of the hair growth period. The hair papilla is the lowermost end of the hair follicle, with capillaries and nerve endings attached. At the base of the hair follicle, the epidermal cells divide and differentiate. These epidermal cells differentiate in different ways, forming different components of the hair (such as cortex, epidermis and medulla), and the outermost cells form the inner root sheath. The sebaceous gland functions to secrete sebum, which is squeezed out through the sebum tube, and when hair passes through the sebum tube, the sebum squeezed out through the sebum tube is carried away. Sebum provides natural protection to the hair, imparting shine and water-resistance to the hair.

Scalp and hair disorders caused by scalp dysbiosis include dandruff, seborrheic dermatitis, tinea capitis, psoriasis, alopecia, and the like. Although these diseases are not serious medically and generally do not have great influence on the physical health of people, the diseases easily cause psychological stress to patients, and cause social problems and psychological problems. Therefore, how to better maintain scalp and hair health is also a medical problem to be solved, wherein androgenetic alopecia (AGA) caused by excessive male androgen secretion is the most common and serious alopecia problem at present. When fibroblasts are cultured in an environment with high androgen content, the content of secreted Fibroblast Growth Factors (FGF) is reduced, the service life of the FGF is short, so that the cell division speed of a hair root part is reduced, and even the cell division speed of the hair root part begins to die, so that the hair grows into a catagen or a resting stage, and the hair is shed in the long term. Therefore, the design and preparation of a material for effectively stabilizing the FGF factor have important significance.

Polyphosphoric acid (Poly P) is a linear polymer formed by connecting orthophosphate monomers through high-energy anhydride bonds, is the third main polyanion behind intracellular DNA and RNA, exists in most prokaryotes and all eukaryotic cells, and has an important physiological regulation function. It was reported that FGF-2 retention decreased to less than 10% after 25 hours of culture in the absence of polyphosphoric acid, which greatly affected the mitogenic activity of FGF-2. The existence of Poly P can improve the stability of FGF and prolong the service life, and the retention amount of FGF-2 after 25 hours is far higher than 10%, so that FGF-2 can have higher mitogenic activity in the presence of polyphosphoric acid compared with the case of no polyphosphoric acid.

The existing preparation method of the polyphosphate is mainly obtained by biological secretion purification and chain cutting methods, the preparation process is complex and tedious, materials with different chain lengths can generate different performances, and the design and optimization of the preparation process of the polyphosphate and the discussion of the preparation process of the polyphosphate have important significance on the regulation and control of hair growth.

Therefore, the polyphosphate capable of regulating and controlling the hair growth and the preparation method thereof are provided.

Disclosure of Invention

It is an object of the present invention to solve or at least alleviate problems in the prior art.

In order to achieve the purpose, the invention is realized by the following technical scheme:

a polyphosphate having hair growth regulating properties, comprising:

dihydrogen phosphate salt: the components of the compound comprise one or more of sodium dihydrogen phosphate, potassium dihydrogen phosphate and calcium dihydrogen phosphate, and the total proportion of the components of the dihydrogen phosphate is 20-100%;

dibasic acid phosphate: the components of the compound comprise one or more of disodium hydrogen phosphate, dipotassium hydrogen phosphate and dicalcium hydrogen phosphate, and the total proportion of the disodium hydrogen phosphate is 0-80%.

Alternatively, the total calcium phosphate monobasic is 25-35% and the total potassium phosphate dibasic is 65-75%.

A method for preparing polyphosphate for regulating hair growth comprises the following steps:

firstly, putting dihydric phosphate and/or dibasic phosphate powder into a muffle furnace at 400-500 ℃ for preheating treatment for 1-2 hours;

step two, heating the product in the step one to 1300 ℃ at the temperature of 1000 ℃, and then carrying out constant temperature treatment for 8-12 hours;

step three, quenching the generated article in the step two at 40-60 ℃ to obtain a product, and performing ball milling according to the material use performance to obtain samples with different scales.

An application method of polyphosphate for regulating and controlling hair growth is to apply the polyphosphate to a hair loss prevention and hair growth promotion product.

A method for using hair loss preventing and hair growth promoting product containing polyphosphate comprises uniformly applying the product containing polyphosphate on the surface of hair loss fur, and massaging for absorption.

The embodiment of the invention provides a polyphosphate capable of regulating and controlling hair growth and a preparation method thereof. The method has the following beneficial effects: from experimental examples 1 and 2, it can be seen that the polyphosphate produced by the present invention has the best effect on normal SD rats, and the polyphosphate produced by the present invention in example 9 has a significant effect on seborrheic alopecia, and the polyphosphate produced by the present invention in example 9 has a significantly increased number of hairs in the area after 15 days of smearing and massaging treatment, and has a tough hair quality and a normal hair follicle morphology, which most approaches the number and quality of hairs in normal SD rats, and the skin treated with the product of example 9 has a complete and healthy hair follicle status as illustrated in slice images.

Drawings

FIG. 1 is a graph of hair growth in normal SD rats.

FIG. 2 is a statistical chart of hair growth scores of normal SD rats.

FIG. 3 is a graph showing the hair growth of SD rats overproof androgen.

FIG. 4 statistical chart of hair growth scores of SD rats overproof androgen.

FIG. 5 HE staining pattern of SD rat skin treated with different materials.

Detailed Description

In order to make the objects, technical solutions and advantages of the embodiments of the present invention clearer, the technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are some, but not all, embodiments of the present invention. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.

Referring to fig. 1-5, a polyphosphate for regulating hair growth, comprising:

dihydrogen phosphate salt: the components of the compound comprise one or more of sodium dihydrogen phosphate, potassium dihydrogen phosphate and calcium dihydrogen phosphate, and the total proportion of the components of the dihydrogen phosphate is 20-100%;

dibasic acid phosphate: the components of the compound comprise one or more of disodium hydrogen phosphate, dipotassium hydrogen phosphate and dicalcium hydrogen phosphate, and the total proportion of the disodium hydrogen phosphate is 0-80%.

Example 2

A method for preparing polyphosphate for regulating hair growth comprises the following steps:

firstly, putting dihydric phosphate and/or dibasic phosphate powder into a muffle furnace at 400-500 ℃ for preheating treatment for 1-2 hours;

step two, heating the product in the step one to 1300 ℃ at the temperature of 1000 ℃, and then carrying out constant temperature treatment for 8-12 hours;

step three, quenching the generated article in the step two at 40-60 ℃ to obtain a product, and performing ball milling according to the material use performance to obtain samples with different scales.

The process of the invention is a micromolecule synthesis method, and has the advantages of simple equipment and process, short synthesis time, no biological culture and higher reliability.

Compared with the prior art that the preparation process is a biological synthesis method, the process is complex, the reliability is low, and the links needing special attention are reaction temperature and time control;

example 3

An application method of polyphosphate for regulating and controlling hair growth is to apply the polyphosphate to a hair loss prevention and hair growth promotion product.

Example 4

The application method of the anti-hair loss and hair growth product containing polyphosphate comprises the steps of uniformly smearing the anti-hair loss and hair growth product containing polyphosphate on the surface of hair loss fur, and massaging and absorbing the product.

Examples 5 to 9

Examples 5-9 were prepared according to the method of example 2 with varying ratios of monobasic and dibasic phosphates as shown in the following table.

The hair growth cycle is usually 5-6 years, with four stages of growth, aging, dormancy and loss. Once the growth phase enters the catagen or telogen phase, hair loss occurs. The hair in the growth stage is subjected to growth instructions sent by cells in the papilla of the hair to regulate and control the growth of peripheral cells, wherein the growth instructions are carried by hair growth information protein (FGF), but the life and the stability of the hair are extremely poor, and if the FGF can be stabilized, the hair growth period can be prolonged, and the alopecia can be prevented. Polyphosphate (Poly P) is widely distributed in eukaryotes and prokaryotes, and is a linear polymer formed by polymerizing dozens to hundreds of inorganic phosphate monomers through high-energy phosphate bonds. Poly P can affect the virulence of bacteria and help them resist environmental stress stimuli. In eukaryotic cells, Poly P is associated with nucleoli transcription, promotes coagulation and cellular differentiation, protects growth factors, regulates pro-inflammatory responses, and is associated with bone remodeling, mineralization, and demineralization. Wherein a specific chain length (generally 75) has been confirmed to have a role of regulating and stabilizing FGF protein, it can be seen that the effect of the combination of example 9 on hair growth is more significant.

Experimental example 1

An animal experiment was conducted using sample 1 obtained as prepared in example 9, and the hair-regulating effect on normal mice and mice whose androgen secretion exceeds the standard was verified. Old age

Adult SD rats were selected for 6 rats and divided into two groups:

a first group: mice were injected with normal saline as a group of normal hormone levels;

second group: the mice simulate the situation that the secretion of the male hormone exceeds the standard by injecting the male hormone.

All rats were shaved back using an electric hair cutter the first day from the start of the experiment to expose the operating area, then applied with depilatory cream to the shaved skin and left for about 5min, then hung with a spatula and cleaned of residual depilatory cream on the peripheral hair using gauze impregnated with deionized water.

The dosage of the normal saline injected into the first group of mice is 500 mu L per day;

the second group of rats injected with androgen-simulated androgen at a dose of 5mg/kg daily, and prepared in glycerol solution.

200 μ L of the corresponding material was applied daily to each area of the shaved area of each mouse from the first day of the start of the experiment:

wherein BJ represents the existing market alopecia preventing mother liquor;

JP represents a polyphosphoric acid solution obtained in example 5 of the present invention;

CP represents a polyphosphoric acid solution obtained in example 9 of the present invention;

all applications were evenly applied with a cotton swab and the application site was gently massaged until most of the visible absorption was observed. During the experiment, the hair growth condition of each mouse was recorded by photographing every day, and the evaluation was performed according to the degree of hair exuberance, and the final results are shown in fig. 1 to 4.

The invention has the following beneficial effects:

for normal SD rats, the hair growth of the SD rats can be accelerated by three different products, and the polyphosphate produced in the embodiment 9 of the invention has the best effect.

It can be seen that the polyphosphate produced in example 9 of the present invention has significant effect on seborrheic alopecia, and after 15 days of smearing and massaging treatment, the number of hairs in the area is increased significantly, the hair quality is tough, the hair follicle morphology is normal, and the number and the quality of the hairs are most similar to those of normal SD rats.

Experimental example 2

Fresh skin tissues of mice in experiment example 1 were taken and put into a prepared fixative (10% formalin) to denature and coagulate proteins of tissues and cells, thereby preventing autolysis or bacterial decomposition after cell death and maintaining the original morphological structure of cells. Then, the water in the tissue blocks is gradually removed by using alcohol from low concentration to high concentration as a dehydrating agent. Then placing the tissue block in a transparent agent xylene which is dissolved in alcohol and paraffin for transparency, replacing the alcohol in the tissue block with xylene, and then carrying out wax dipping and embedding. Placing the transparent tissue block in melted paraffin, and placing the tissue block in a paraffin dissolving box for heat preservation. Embedding after the paraffin is completely immersed into the tissue block: preparing a container, pouring melted paraffin, quickly clamping tissue blocks soaked with paraffin and putting the tissue blocks into the container. Cooling and solidifying into blocks. Fixing the embedded wax block on a slicer, cutting into slices of 5-8 μm, ironing in heated water, sticking on a glass slide, and oven drying at 45 deg.C. And finally, HE staining is carried out to increase the color difference of each part of the tissue cell structure, thereby being beneficial to observation. Hematoxylin (H) is a basic dye that stains the nucleus and intracellular ribosome bluish purple, and the structures stained by basic dye are alkalophilic. Eosin (E) is an acid dye that stains cytoplasm red or reddish, and the structure stained by the acid dye is eosinophilic. Before dyeing, the paraffin in the slices is removed by xylene, and then the slices are dyed by high-concentration to low-concentration alcohol and finally distilled water.

The specific operation process of HE dyeing is as follows:

firstly, putting the slices into a hematoxylin water solution for dyeing for a plurality of minutes after the distilled water is added;

② color separation is carried out in acid water and ammonia water, each time is several seconds;

flushing with running water for 1 hr and adding distilled water for some moment;

fourthly, dewatering in 70 percent and 90 percent alcohol for 10 minutes respectively;

fifthly, adding alcohol eosin staining solution to dye for 2-3 minutes.

The dyed slices are dehydrated by pure alcohol and then are transparent by xylene. The transparent sections were dropped with Canadian gum and mounted with a coverslip. After the gum is slightly dried, a label is attached, the section sample can be photographed and observed by using an optical microscope to obtain the appearance of the epidermal tissue, and as shown in fig. 5, the state of the hair follicle of the skin treated by the product of the example 9 is complete and healthy as shown in the section picture.

It is noted that, herein, relational terms such as first and second, and the like may be used solely to distinguish one entity or action from another entity or action without necessarily requiring or implying any actual such relationship or order between such entities or actions. Also, the terms "comprises," "comprising," or any other variation thereof, are intended to cover a non-exclusive inclusion, such that a process, method, article, or apparatus that comprises a list of elements does not include only those elements but may include other elements not expressly listed or inherent to such process, method, article, or apparatus. Without further limitation, an element defined by the phrase "comprising an … …" does not exclude the presence of other identical elements in a process, method, article, or apparatus that comprises the element.

The above examples are only intended to illustrate the technical solution of the present invention, but not to limit it; although the present invention has been described in detail with reference to the foregoing embodiments, it will be understood by those of ordinary skill in the art that: the technical solutions described in the foregoing embodiments may still be modified, or some technical features may be equivalently replaced; and such modifications or substitutions do not depart from the spirit and scope of the corresponding technical solutions of the embodiments of the present invention.