阅读说明：本技术 一种辅助睡眠的功能性发酵酸奶饮品的制备方法 (Preparation method of functional fermented yoghourt drink for assisting sleep ) 是由 祝逢才 王祖东 薛佩佩 于 2020-06-04 设计创作，主要内容包括：一种辅助睡眠的功能性发酵酸奶饮品的制备方法。本发明提供了一种含有γ-氨基丁酸、L-茶氨酸、酸枣仁提取物的功能性发酵酸奶。其制备工艺,每338ml发酵酸奶中含100mgγ-氨基丁酸、100mgL-茶氨酸、25mg酸枣仁提取物,将这些有功能效果的原料,再加入奶粉,保加利亚乳杆菌、嗜热链球菌等制备成助眠发酵酸奶饮料。该饮料口感好,易吸收,而且可有效帮助提高睡眠质量水平,作为一种新型饮品来普及和提升人们的健康。(A preparation method of functional fermented yogurt beverage for assisting sleep is provided. The invention provides a functional fermented yoghourt containing gamma-aminobutyric acid, L-theanine and spina date seed extracts. The preparation process comprises the steps of adding 100mg of gamma-aminobutyric acid, 100mg of L-theanine and 25mg of spina date seed extract into 338ml of fermented yogurt, and adding milk powder, lactobacillus bulgaricus, streptococcus thermophilus and the like into the raw materials with functional effects to prepare the sleep-aiding fermented yogurt drink. The beverage has good taste and easy absorption, and can effectively improve sleep quality, and be popularized as a novel beverage and improve health of people.)

1. The invention relates to a preparation method of a functional fermented yoghourt drink for assisting sleep, wherein the functional fermented yoghourt is prepared from gamma-aminobutyric acid, L-theanine, a spina date seed extract and fermented yoghourt; the wild jujube seed extract with the purity of 99 percent, namely gamma-aminobutyric acid, L-theanine and the total saponin number of more than or equal to 2 percent is selected.

2. Wherein, each 1000ml of the functional fermented yoghourt contains the following components by weight: : 296mg of gamma-aminobutyric acid, 296mg of L-theanine and 74mg of spina date seed extract.

3. The specific manufacturing process is as follows:

the water for the ingredients is purified water, and the cleaning and the disinfection in the production process are carried out according to the technological operation of the milk-containing beverage: (1) preparing primary pulp: weighing materials and feeding, namely adding whole milk powder, skim milk powder, lactobacillus bulgaricus and streptococcus thermophilus; secondly, stirring, namely uniformly mixing the added materials; thirdly, blending, namely adding RO filtered purified water, and adding white granulated sugar, food additives, gamma-aminobutyric acid, L-theanine and spina date seed extracts; (2) homogenizing and sterilizing: homogenizing at low pressure of 2MPa and high pressure of 20 MPa; secondly, UHT sterilization, UHT98 ℃ sterilization; (3) storage, filling and cooling: storing the first material liquid, filling in the second step, inverting the bottle and sterilizing for 40s in the third step, and cooling in the fourth step; (4) and (5) spraying code, labeling, boxing and warehousing.

Technical Field

The invention relates to a preparation method of a functional fermented yoghourt drink for assisting sleep

In particular to a sleep-aiding fermented yoghourt beverage which takes gamma-aminobutyric acid, L-theanine and a spina date seed extract as sleep-aiding factors.

Background

In recent years, people suffering from insomnia or sleep problems have been increasing with the improvement of living standard, the change of dietary structure and the increase of living pressure. According to the investigation of the world health organization, people with about 1/3 sleep disorder all have sleep disorder worldwide, and people with various sleep disorders in China have the sleep disorder rate as high as 38.2 percent and the sleep disorder rate is higher than 27 percent of the world. Insomnia can bring many negative effects to people's life, such as weakness, dizziness, amnesia, mental strength reduction, irritability, and mental problems of various organ functional disorders, depression, anxiety and the like. Therefore, the natural sleep-aiding functional beverage without side effect has wide market prospect.

Gamma-aminobutyric acid, also known as GABA, is a natural active ingredient widely distributed in animals and plants. Gamma-aminobutyric acid (GABA) was certified by the U.S. Food and Drug Administration (FDA) GRAS in month 7 of 2008. In 2009, 9.27 days, the Ministry of health in China approves gamma-aminobutyric acid as a new resource food. Gamma-aminobutyric acid has the following functions: (1) the function of reducing blood pressure: the gamma-aminobutyric acid can promote cerebral blood flow, increase oxygen supply and promote cerebral metabolism, and on the other hand, the gamma-aminobutyric acid acts on the vasomotor center of the medulla oblongata to reduce blood pressure, inhibit secretion of anti-diuretic hormone, expand blood vessels and reduce blood pressure. (2) The sleep aiding function is as follows: the gamma-aminobutyric acid is a main inhibitory neurotransmitter of the central nervous system, and can relieve physical and mental pressure, relax mood, help fall asleep, improve sleep quality, improve depression and improve memory. To evaluate the efficacy of gaba for sleep improvement, a placebo-controlled study was conducted by electroencephalography to measure brain waves after subjects had taken 100mg of gaba or placebo. The study results showed that gaba was effective in shortening the time to sleep to 5 minutes and improving the high quality sleep time compared to the placebo control group. Questionnaires show that subjects taking gamma-aminobutyric acid have improved sleep quality, especially better energy after waking. (3) The gamma-aminobutyric acid provides another way for the tricarboxylic acid cycle of glutamic acid, so that the brain tonic wine can effectively improve cerebral blood circulation, increase oxygen supply and promote the metabolic function of the brain, and can be used for treating headache, tinnitus, blurred consciousness and other diseases caused by cerebral apoplexy, head trauma sequelae, cerebral arteriosclerosis sequelae and the like. (4) The pressure-relieving effect is achieved by carrying out human body tests on the highest and longest rice-leaved garlics hanger (54 meters high and 297 meters long) in Japan, wherein an experimental group takes 1 gram of GABA and a control group only drinks water, saliva is collected once before, in the middle and after crossing the hanger, and changes of CgA (Chromogranin A) in the saliva are tested, so that substances released from the saliva when mental stress occurs are increased, and the quantity of the substances is increased, and as a result, a tested person taking gamma-aminobutyric acid is found to have less CgA secretion compared with the control group, and the pressure-relieving effect is shown. (5) The gamma-aminobutyric acid has the functions of beautifying and losing weight, can prevent skin aging, prevent arteriosclerosis and improve lipid metabolism, and can be clinically used as a medicament for improving various symptoms caused by cerebral arteriosclerosis, improving hyperlipidemia and preventing obesity. In addition, the gamma-aminobutyric acid also has the functions of improving liver and kidney functions, resisting aging, controlling asthma, improving lipid metabolism, preventing obesity, preventing arteriosclerosis, dispelling the effects of alcohol, removing odor and the like.

L-theanine has the reputation of "happy amino acid", is a unique free amino acid in tea leaves, and is separated from Yulu tea in 1949. Has effects in tranquilizing mind, relaxing, improving sleep, lowering blood pressure, resisting depression and tumor, and enhancing immunity. In recent years, theanine, which is called "natural sedative", has become one of the most popular natural health products internationally. L-theanine has the following functions: (1) the antihypertensive effect is shown in the recent research that the blood pressure of the congenital hypertension mouse can be remarkably reduced by feeding the mouse with 200g/kg of L-theanine. Experiments in human bodies also find that the blood pressure can be reduced by injecting 200mg of L-theanine once; in addition, L-theanine can also reduce the side effect of blood pressure increase caused by caffeine intake (dosage of 250 mg); (2) relaxation effect, and related researches prove that the L-theanine can promote the generation of alpha waves (relaxation state) on the surface of the brain to enable a human body to have a comfortable and pleasant feeling, but the L-theanine cannot cause the increase of the number of 0 waves generated in a sleeping state, so that the L-theanine can not enable the human body to sleep while stabilizing the emotion. The L-theanine not only has some anti-depression relaxation effects in a rest state, but also can achieve an anti-stress effect by inhibiting the excitation of cerebral cortex nerves, and relieve the physiological and psychological pressure of a human body in a loaded pressure state; (3) the L-theanine is an analogue of excitatory transmitter L-glutamic acid, and can compete with L-glutamic acid for receptor to achieve the protection effect on brain tissue. (4) Anti-fatigue effect, which is probably related to that L-theanine can inhibit the secretion of 5-hydroxytryptamine which is a central neurotransmitter in brain, promote the secretion of catecholamine which is a peripheral neurotransmitter and increase the dopamine content in brain tissues; (5) the L-theanine with the anti-tumor effect is taken as a derivative of the L-glutamine, and can achieve the purpose of inhibiting the growth of tumor cells by interfering the metabolism of the L-glutamine of the tumor cells; (6) in addition, theanine also has effects of improving learning ability, enhancing immunity, improving menstrual period syndrome, reducing body fat, resisting aging, resisting diabetes, reducing abdominal fat, reducing blood lipid and protecting liver.

Spina date seed, also known as spina date seed, jujube kernel, spina date seed and the like, is a seed of the Rhamnaceae plant spina date seed. Sweet in taste and neutral in nature; enter heart, gallbladder and liver meridians. Has the effects of nourishing liver, calming heart, tranquilizing mind, and arresting sweating. It is mainly used for treating symptoms such as vexation, insomnia, palpitation, polydipsia, sweating due to debility, etc. The semen Ziziphi Spinosae extract contains two trialkyl compounds, semen Ziziphi Spinosae saponin and flavone compounds, and has tranquilizing effect. The main effective components in the spina date seed extract are spina date seed saponin A and spina date seed saponin B, which can improve the GABA receptor expression level of brain tissues, combine the research of the Chinese and western aspects, can nourish the heart and calm the nerves, improve the GABA content and promote the GABA receptor expression, thereby playing the roles of relieving pressure, soothing the heart and sleeping. The method can be effectively applied to symptoms such as blindness, dreaminess, mental fatigue and the like caused by overstrain, mental stress and large working pressure by measuring the disappearance and recovery time of the righting reflex of the drug-filled mice.

In consideration of the above effects of gamma-aminobutyric acid, L-theanine and the wild jujube seed extract, the gamma-aminobutyric acid, L-theanine and the wild jujube seed extract are very important in the development of beverage products, and are nontoxic and safe as functional foods. The drink has good taste, the social significance and the economic significance of the product are very great, and the development of the drink beneficial to sleep brings great influence on human health.

Disclosure of Invention

In order to solve the problems, the invention provides a preparation method of sleep-aiding fermented yoghourt containing gamma-aminobutyric acid, L-theanine and spina date seed extracts, so as to help improve the function of sleep quality (sleep depth and waking awakening), and the yoghourt is fragrant, mellow and delicious in taste and suitable for popularization and drinking.

The invention is realized by the following technical scheme:

a functional fermented yogurt for assisting sleep is prepared from gamma-aminobutyric acid, L-theanine, semen Ziziphi Spinosae extract, purified water, skimmed milk powder, various fermented yogurt and food additives.

In order to verify the combined action of different contents of functional components, the following 3 different proportions are prepared for every 1000ml of fermented yoghurt, and the specific component proportion conditions are as follows:

	gamma-aminobutyric acid	L-theanine	Spina date seed saponin
				First group	148mg	148mg	88mg
Second group	296mg	296mg	74mg
				Third group	222mg	222mg	60mg

The beverage with the three components added according to different proportions is applied to 100 people with sleep problems and the effect after drinking is recorded. The trial results are as follows: in the first group, 68 people have obvious sleep promoting effect after drinking; in the second group, 96 people have obvious sleep promoting effect after drinking; the third group 85 has obvious sleep promoting effect after drinking. It can be seen that the second group of ratios is the best combination.

The gamma-aminobutyric acid (GABA) contained in the beverage formula belongs to amino acid derivatives. It is a nonprotein amino acid which plays a role in tranquilizing nerves in animals, and is mainly prepared by decarboxylation of glutamic acid in animals. The gamma-aminobutyric acid is a central nerve sedative, and researches show that the gamma-aminobutyric acid has the effects of calming and helping sleep, resisting convulsion, reducing blood pressure, improving brain activity, nourishing nerve cells, promoting auxin secretion, protecting liver and benefiting kidney and the like. The product can be widely applied to beverage, convenience food, baked food and sports food ingredients, and simultaneously has the following advantages: pure natural, no radiation, no pollution, no additive and the like, and is suitable for drinking.

The L-theanine contained in the beverage formula is a special free amino acid naturally existing in tea leaves, and is widely used in countries and regions such as Europe and America, Japan, Taiwan China and the like as a novel food additive. L-theanine has good stability (under common food processing conditions such as sterilization, pH and heating), and thus has a wide application range. At present, chocolate, jelly, pudding, chewing gum, health tea and various refreshing beverages to which theanine is added have been developed in japan.

The spina date seed extract contained in the beverage formula is prepared by extracting dried mature seeds of a Rhamnaceae plant ziziphus jujube, belongs to a medicine-food homologous plant, has high total saponin content of more than or equal to 2 percent, has high fluidity, can be subjected to water-soluble treatment, and can be used as a food raw material, a food ingredient, a spina date seed solid beverage raw material, a liquid beverage raw material, a tablet candy raw material and the like.

The invention has the following advantages and beneficial effects: the fermented yoghourt can be drunk every day, is recommended to be drunk half an hour before sleeping, can effectively relieve difficulty in falling asleep, improve sleep quality, enhance sleep depth and relieve fatigue feeling during waking, is sweet and mellow in mouthfeel, contains lactic acid bacteria, and has a certain protection effect on intestinal tracts.

The specific manufacturing process is as follows:

the water for the ingredients is purified water, and the cleaning and the disinfection in the production process are carried out according to the technological operation of the milk-containing beverage. (1) Preparing primary pulp: weighing materials and feeding, namely adding whole milk powder, skim milk powder, lactobacillus bulgaricus and streptococcus thermophilus; secondly, stirring, namely uniformly mixing the added materials; thirdly, blending, namely adding RO filtered purified water, and adding white granulated sugar, food additives, gamma-aminobutyric acid, L-theanine and spina date seed extracts; (2) homogenizing and sterilizing: homogenizing at low pressure of 2MPa and high pressure of 20 MPa; secondly, UHT sterilization, UHT98 ℃ sterilization; (3) storage, filling and cooling: storing the first material liquid, filling in the second step, inverting the bottle and sterilizing for 40s in the third step, and cooling in the fourth step; (4) and (5) spraying code, labeling, boxing and warehousing.

FIG. 1 is a chemical structural formula of gamma-aminobutyric acid; FIG. 2 is a chemical structural formula of L-theanine; FIG. 3 is a preparation process diagram of fermented yogurt.

Example (b):

collecting 338ml fermented yogurt, collecting gamma-aminobutyric acid 100mg, collecting L-theanine 100mg, collecting semen Ziziphi Spinosae extract 25mg, mixing and dissolving, and making into bottle or canned beverage according to the existing filling process. Some preservatives can be added in a proper amount for storage and transportation, other ingredients such as fruit juice and the like can also be added to increase the variety and selling points, and meanwhile, the beverage can be used as a novel beverage to spread and improve the health quality of people.