阅读说明：本技术 一种灵芝孢子油软胶囊的制备工艺 (Preparation process of ganoderma lucidum spore oil soft capsule ) 是由 施小燕 于 2021-07-27 设计创作，主要内容包括：本发明涉及一种灵芝孢子油软胶囊的制备工艺,其工艺步骤如下：取灵芝孢子原料经超微粉碎破壁处理后过筛、制粒为灵芝孢子粒,超临界萃取、分离获得灵芝孢子油,与灵芝孢子粉、大豆磷脂、活性肽、二十二碳六烯酸、维生素配制为软胶囊内容物,取软胶囊内容物和密封层胶浆滴胶成型、流化床喷雾依次与结合层胶浆和缓释层胶浆包衣,干燥定型、获得成品,密封层胶浆、结合层胶浆和缓释层胶浆包裹灵芝孢子油复配软胶囊内容物、制成多层胶壳的灵芝孢子油软胶囊,密封层胶浆阻断软胶囊内容物与明胶中的氨基酸残基自氧化或交联作用,结合层胶浆增加结合性、避免分层,缓释层胶浆抗氧化、释放可控,避免崩解时间延长、提高产品质量稳定性和生物活性。(The invention relates to a preparation process of a ganoderma lucidum spore oil soft capsule, which comprises the following process steps: micronizing Ganoderma spore, breaking cell wall, sieving, granulating to obtain Ganoderma spore granule, performing supercritical extraction, separating to obtain Ganoderma spore oil, mixing with Ganoderma spore powder, soybean phospholipid, active peptide, docosahexaenoic acid, and vitamins to obtain soft capsule content, dripping the soft capsule content and sealing layer mucilage for molding, spraying with fluidized bed, sequentially coating with bonding layer mucilage and sustained release layer mucilage, drying, and shaping to obtain the final product, coating Ganoderma spore oil with the sealing layer mucilage, bonding layer mucilage and sustained release layer mucilage to obtain the final product, and making into multi-layer Ganoderma spore oil soft capsule.)

1. A preparation process of a ganoderma lucidum spore oil soft capsule is characterized by comprising the following process steps:

s1: taking a ganoderma spore raw material, carrying out superfine grinding and wall breaking treatment, sieving and granulating to obtain ganoderma spore granules;

s2: extracting Ganoderma spore granule with supercritical extraction, separating to obtain Ganoderma spore oil, mixing Ganoderma spore oil with Ganoderma spore powder, soybean phospholipid, active peptide, docosahexaenoic acid, and vitamins to obtain soft capsule content;

s3: dissolving the soft capsule shell material into glue, stirring uniformly for defoaming to prepare sealing layer mucilage, binding layer mucilage and slow release layer mucilage, taking the soft capsule content and the sealing layer mucilage for glue dripping molding to prepare the ganoderma spore oil soft capsule, cooling, drying and shaping, then coating with the binding layer mucilage and the slow release layer mucilage in sequence by fluidized bed spraying, drying and shaping, and cleaning to obtain the finished product.

2. The process for preparing the ganoderma lucidum spore oil soft capsule according to claim 1, wherein the supercritical CO is adopted during the superfine grinding and wall breaking treatment₂Carrying the Ganoderma spore raw material at 25-45MPa and-10-15 deg.C, impacting hard target material, jet-pulverizing, reducing pressure to 8-10Mpa, separating to obtain Ganoderma spore powder, sieving with fineness of 200 mesh or more, granulating to obtain Ganoderma spore granule of 40-120 mesh, and vacuum drying.

3. The process for preparing the ganoderma lucidum spore oil soft capsule according to claim 1, wherein the supercritical extraction is carried out in an extraction kettle at the pressure of 28-42 MPa, the temperature of 40-55 ℃ and supercritical CO₂The flow rate is 30-45Kg/h, the ultrasonic frequency is 30-60 kHz, the ultrasonic power is 100-800W, and supercritical CO is carried out₂Extracting for 3-6 hr, cooling in one-stage or two-stage separation kettle to 5-10 deg.C, and separating and collecting Ganoderma spore oil under 8-10 Mpa.

4. The preparation process of the ganoderma spore oil soft capsule according to claim 1, wherein the content of the soft capsule comprises the following components in percentage by mass: 75-90: 6-8:0.1-5:0.05-2: 0.05-2: 0.05-0.5, and mixing uniformly.

5. The preparation process of the ganoderma lucidum spore oil soft capsule according to claim 1, wherein the active peptide is one or more of momordica charantia peptide, sea cucumber peptide, seaweed peptide, soybean peptide and walnut peptide, and the vitamin is one or more of fat-soluble vitamin A, vitamin D, vitamin E and vitamin K.

6. The process for preparing the ganoderma lucidum spore oil soft capsule according to claim 1, wherein one or more of butyl hydroxy anisole, 2, 6-di-tert-butyl-4-methylphenol, tert-butyl hydroquinone and propyl gallate in the total mass ratio of 0.01-0.5% are further added to the content of the soft capsule.

7. The preparation process of the ganoderma spore oil soft capsule according to claim 1, wherein the soft capsule shell material of the sealing layer mucilage is hydroxypropyl methylcellulose, a plasticizer and deionized water according to a mass ratio of 50-60: 15-20: 20-40, wherein the soft capsule shell material of the bonding layer mucilage is a high molecular compound, a thickening agent and deionized water according to the mass ratio of 40-60: 10-15: 20-30, the soft capsule shell material of the sustained-release layer mucilage is pullulan polysaccharide, gelatin, a plasticizer, a disintegrating agent, an opacifier and deionized water according to the mass ratio of 20-30:25-35: 15-25: 7-12:0.1-0.5: 15-20.

8. The process for preparing the ganoderma lucidum spore oil soft capsule according to claim 7, wherein the plasticizer is one or more of glycerol, agar, polyethylene glycol 400 and phthalate, the high molecular compound is one or more of guar gum, chitin, pectin and gum arabic, and the thickener is one or more of propylene glycol alginate and sodium citrate.

9. The process for preparing the ganoderma lucidum spore oil soft capsule according to claim 8, wherein the disintegrant is one or more of starch and sodium alginate, and the opacifier is one or more of carotene, curcumin, carmine pigment and brilliant blue pigment.

10. The process for preparing the ganoderma lucidum spore oil soft capsule according to any one of claims 1 to 9, wherein the thickness of the content of the soft capsule wrapped by the sealing layer mucilage of the ganoderma lucidum spore oil soft capsule during the glue dripping is 80 to 100 μm, the coating thickness of the binding layer mucilage is 4 to 8 μm, and the coating thickness of the slow release layer mucilage is 50 to 150 μm.

Technical Field

The invention relates to a preparation process of a ganoderma spore oil soft capsule, belonging to the technical field of spore oil soft capsules.

Background

The ganoderma spore oil soft capsule is prepared by using wall-broken ganoderma spore powder as a raw material and refining liposoluble substances extracted by supercritical CO2 fluid, integrates various effective active ingredients such as triterpenoid ganoderma acid, unsaturated fatty acid, organic germanium, trace elements and the like in the protoplasm of the ganoderma spore powder, and is a health-care product mainly used for auxiliary treatment and daily health care of tumor patients. In the prior art, the ganoderma lucidum spore oil soft capsule is subjected to wall breaking treatment by a superfine pulverizer and then is filtered and extracted, and because the refining degree of spore powder is higher during extraction, the spore powder enters a separation kettle along with gas and is easy to block a pipeline, so that the extraction is ineffective; the soft capsule filling oil particles are dripped on the surface of gelatin, the gelatin skin is sealed when the gelatin particles descend under the action of gravity acceleration, the gelatin particles form a spherical shape under the action of surface tension and gradually solidify to form a soft capsule, the polypeptide fragments hydrolyzed by gelatin collagen and amino acid molecules generate cross-linking reaction, the disintegration time of the soft capsule is prolonged during storage, and the problem that the stability and the quality of a product are influenced because the capsule shell and the content ganoderma lucidum steamed stuffed bun oil contact the inner wall to form a membranous substance.

Disclosure of Invention

The invention aims to provide a preparation process of a ganoderma lucidum spore oil soft capsule aiming at the defects of the prior art, the sealing layer mucilage, the binding layer mucilage and the slow release layer mucilage wrap the contents of the ganoderma lucidum spore oil compound soft capsule to prepare the ganoderma lucidum spore oil soft capsule with a multilayer capsule shell, the autoxidation or the crosslinking action is blocked, the disintegration time is prevented from being prolonged, and the quality stability and the bioactivity of the product are improved.

The invention is realized by the following technical scheme:

a preparation process of a ganoderma lucidum spore oil soft capsule comprises the following process steps:

s1: taking a ganoderma spore raw material, carrying out superfine grinding and wall breaking treatment, sieving and granulating to obtain ganoderma spore granules;

supercritical CO is adopted during wall breaking treatment of superfine grinding₂Carrying a ganoderma lucidum spore raw material through a nozzle under the pressure of 25-45MPa and the temperature of-10-15 ℃ to impact a hard target material for jet flow crushing, reducing the pressure to 8-10MPa, separating to obtain ganoderma lucidum spore powder, sieving to obtain ganoderma lucidum spore powder with the fineness of more than or equal to 200 meshes, granulating to obtain ganoderma lucidum spore granules with the granularity of 40-120 meshes through a granulating machine, and vacuum drying;

s2: performing supercritical extraction and separation on ganoderma spore particles to obtain ganoderma spore oil, wherein the ganoderma spore oil, the ganoderma spore powder, the soybean phospholipid, the active peptide, the docosahexaenoic acid and the vitamins are mixed according to the mass ratio: 75-90: 6-8:0.1-5:0.05-2: 0.05-2: 0.05-0.5, and mixing to obtain soft capsule content;

in the supercritical extraction, supercritical CO is carried out in an extraction kettle at the pressure of 28-42 MPa and the temperature of 40-55 DEG C₂The flow rate is 30-45Kg/h, the ultrasonic frequency is 30-60 kHz, the ultrasonic power is 100-800W, and supercritical CO is carried out₂Extracting for 3-6 hr, cooling in one-stage or two-stage separation kettle to 5-10 deg.C, and separating and collecting Ganoderma spore oil under 8-10 Mpa;

the active peptide is one or more of bitter gourd peptide, sea cucumber peptide, seaweed peptide, soybean peptide and walnut peptide, and the vitamin is one or more of fat-soluble vitamin A, vitamin D, vitamin E and vitamin K;

the soft capsule content also comprises one or more of butyl hydroxy anisol, 2, 6-di-tert-butyl-4-methylphenol, tert-butyl hydroquinone and propyl gallate which account for 0.01-0.5% of the total mass ratio;

s3: melting the soft capsule shell material at 60-80 ℃, uniformly stirring, preserving heat for 1-2h, standing and defoaming to prepare sealing layer mucilage, binding layer mucilage and slow release layer mucilage, wherein the soft capsule shell material of the sealing layer mucilage is hydroxypropyl methyl cellulose, plasticizer and deionized water according to the mass ratio of 50-60: 15-20: 20-40, wherein the soft capsule shell material of the bonding layer mucilage is a high molecular compound, a thickening agent and deionized water according to the mass ratio of 40-60: 10-15: 20-30, the soft capsule shell material of the sustained-release layer mucilage is pullulan polysaccharide, gelatin, a plasticizer, a disintegrating agent, an opacifier and deionized water according to the mass ratio of 20-30:25-35: 15-25: 7-12:0.1-0.5: 15-20;

the plasticizer is one or more of glycerol, agar, polyethylene glycol 400 and phthalate, the high molecular compound is one or more of guar gum, chitin, pectin and gum arabic, and the thickener is one or more of propylene glycol alginate and sodium citrate;

the disintegrating agent is one or more of starch and sodium alginate, and the opacifier is one or more of carotene, curcumin, carmine pigment and bright blue pigment;

and (3) dripping the soft capsule content and the sealing layer mucilage for forming to prepare the ganoderma lucidum spore oil soft capsule, wherein the thickness of the soft capsule content wrapped by the sealing layer mucilage is 80-100 mu m, cooling, drying and shaping, then sequentially coating with the binding layer mucilage and the slow release layer mucilage by spraying through a fluidized bed, the coating thickness of the binding layer mucilage is 4-8 mu m, the coating thickness of the slow release layer mucilage is 50-150 mu m, drying, shaping and cleaning to obtain a finished product.

The invention has the beneficial effects that:

(1) performing jet crushing and pressure reduction separation by using supercritical CO2 to obtain ganoderma lucidum spore powder, performing superfine crushing and wall breaking treatment on ganoderma lucidum spores, improving the wall breaking rate, granulating to avoid the problem of extraction failure caused by blocking pipelines due to the fact that the ganoderma lucidum spore powder is too thin during extraction, preferably selecting supercritical extraction conditions, combining ultrasonic-assisted supercritical CO2 extraction to improve the extraction rate, and compounding ganoderma lucidum spore oil, the ganoderma lucidum spore powder, soybean lecithin, active peptide, docosahexaenoic acid and vitamins to enable the biological activity of the contents of the compounded soft capsule to be higher and inhibit oxidative degradation into small molecular chains and capsule shell crosslinking;

(2) the sealing layer mucilage, the binding layer mucilage and the slow release layer mucilage are subjected to glue dripping molding and fluidized bed spraying of the binding layer mucilage and the slow release layer mucilage coating to prepare the finished product of the ganoderma spore oil soft capsule with a multilayer capsule shell, the sealing layer mucilage blocks the autoxidation or crosslinking action of the content of the soft capsule and amino acid residues in gelatin, the binding layer mucilage increases the binding property and avoids layering, the slow release layer mucilage is antioxidant and controllable in release, the problems that the disintegration time of the soft capsule is prolonged during storage, and the inner wall of the capsule shell and the content of ganoderma seed oil is contacted to form a membranous substance to influence the stability and quality of the product are avoided, so that the finished product of the ganoderma spore oil soft capsule can be applied to the preparation of anti-tumor food, health-care food or medicine.

Drawings

FIG. 1 is a process flow diagram of the present invention.

Detailed Description

The following description of the embodiments of the present invention will be made with reference to the accompanying drawings.

Example 1:

a preparation process of a ganoderma lucidum spore oil soft capsule comprises the following process steps:

s1: taking a ganoderma spore raw material, carrying out superfine grinding and wall breaking treatment, sieving and granulating to obtain ganoderma spore granules;

supercritical CO is adopted during wall breaking treatment of superfine grinding₂Carrying a ganoderma lucidum spore raw material at the pressure of 35MPa and the temperature of-12 ℃ through a nozzle, impacting a hard target material, carrying out jet flow crushing, reducing the pressure to 8Mpa, separating to obtain ganoderma lucidum spore powder, sieving with the fineness of 300 meshes, granulating the ganoderma lucidum spore powder into 80-mesh ganoderma lucidum spore particles through a granulation machine, and performing vacuum drying;

s2: performing supercritical extraction and separation on ganoderma spore particles to obtain ganoderma spore oil, wherein the ganoderma spore oil, the ganoderma spore powder, the soybean phospholipid, the active peptide, the docosahexaenoic acid and the vitamins are mixed according to the mass ratio: 85: 6:3:0.1: 0.2: 0.3, mixing uniformly to prepare the content of the soft capsule;

the supercritical extraction is carried out in an extraction kettle at 34MPa and 35 deg.C under supercritical CO₂The flow rate is 32Kg/h, the ultrasonic frequency is 50kHz, the ultrasonic power is 600W, and supercritical CO is carried out₂Extracting for 4 hr, cooling to 8 deg.C in the first stage, and separating under 8Mpa to collect Ganoderma spore oil;

the active peptide is composed of balsam pear peptide, sea cucumber peptide and seaweed peptide according to the mass ratio of 1:1:1, and the vitamin is composed of fat-soluble vitamin A and vitamin D according to the mass ratio of 1: 1;

the content of the soft capsule is also added with 0.05 percent of butyl hydroxy anisole and 0.05 percent of 2, 6-di-tert-butyl-4-methylphenol in the total mass ratio;

s3: melting the soft capsule shell material at 62 deg.C, stirring, maintaining the temperature for 1.5 hr, standing, and defoaming to obtain sealing layer mucilage, binding layer mucilage and slow release layer mucilage;

the soft capsule shell material of the sealing layer mucilage is hydroxypropyl methyl cellulose, a plasticizer and deionized water according to the mass ratio of 52: 18: 32, the plasticizer consists of agar and polyethylene glycol 400 according to the mass ratio of 1:1, the soft capsule shell material of the bonding layer mucilage is a high molecular compound, the thickening agent propylene glycol alginate, and deionized water according to the mass ratio of 42: 13: 24, wherein the high molecular compound is guar gum and chitin according to the mass ratio of 1:1, the soft capsule shell material of the sustained-release layer mucilage is pullulan, gelatin, plasticizer glycerol, disintegrant sodium alginate, opacifier carotene and deionized water according to the mass ratio of 23:28: 17: 8:0.2: 16;

and (3) dripping the soft capsule content and the sealing layer mucilage for forming to prepare the ganoderma lucidum spore oil soft capsule, wherein the thickness of the soft capsule content wrapped by the sealing layer mucilage is 85 microns, cooling, drying and shaping, and then sequentially coating with the binding layer mucilage and the slow release layer mucilage through fluidized bed spraying, wherein the coating thickness of the binding layer mucilage is 6 microns, the coating thickness of the slow release layer mucilage is 100 microns, drying, shaping and cleaning to obtain a finished product.

Example 2:

a preparation process of a ganoderma lucidum spore oil soft capsule comprises the following process steps:

s1: taking a ganoderma spore raw material, carrying out superfine grinding and wall breaking treatment, sieving and granulating to obtain ganoderma spore granules;

supercritical CO is adopted during wall breaking treatment of superfine grinding₂Spraying with nozzle at 40MPa and 5 deg.C, impacting hard target material with Ganoderma spore material, jet pulverizing, reducing pressure to 9Mpa, separating to obtain Ganoderma spore powder, sieving with 300 mesh sieve, granulating to obtain 60 mesh Ganoderma spore powderSpore particles are dried in vacuum;

s2: performing supercritical extraction and separation on ganoderma spore particles to obtain ganoderma spore oil, wherein the ganoderma spore oil, the ganoderma spore powder, the soybean phospholipid, the active peptide, the docosahexaenoic acid and the fat-soluble vitamin E are mixed according to the mass ratio: 86: 6:0.1:0.05: 2: 0.3, mixing uniformly to prepare the content of the soft capsule;

the supercritical extraction is carried out in an extraction kettle at 33MPa and 40 deg.C under supercritical CO₂The flow rate is 35Kg/h, the ultrasonic frequency is 45kHz, the ultrasonic power is 500W, and supercritical CO is carried out₂Extracting for 4 hr, cooling to 8 deg.C in the first-stage separation kettle, and separating and collecting Ganoderma spore oil under 10Mpa and 8Mpa in the second-stage separation kettle;

the active peptide is composed of sea cucumber peptide, seaweed peptide and soybean peptide according to the mass ratio of 1:1: 1;

the soft capsule content also comprises 0.08 percent of 2, 6-di-tert-butyl-4-methylphenol and 0.08 percent of tert-butylhydroquinone;

s3: the soft capsule shell material is subjected to glue melting at 68 ℃, uniform stirring, heat preservation for 1h, standing and defoaming to prepare sealing layer glue paste, binding layer glue paste and slow release layer glue paste, wherein the soft capsule shell material of the sealing layer glue paste is hydroxypropyl methyl cellulose, plasticizer and deionized water according to the mass ratio of 55: 18: 32, wherein the plasticizer is polyethylene glycol 400 and phthalic acid ester according to the mass ratio of 1: 1;

the soft capsule shell material of the bonding layer mucilage is a high molecular compound, a thickening agent and deionized water according to the mass ratio of 58: 12: 27, wherein the high molecular compound is composed of chitin and pectin according to the mass ratio of 1:1, and the thickening agent is composed of propylene glycol alginate and sodium citrate according to the mass ratio of 1: 1;

the soft capsule shell material of the sustained-release layer mucilage is prepared from pullulan polysaccharide, gelatin, a plasticizer, disintegrant starch, an opacifier and deionized water according to a mass ratio of 25:32: 19: 10:0.4:18, the plasticizer glycerol and the agar are mixed according to the mass ratio of 1:1, and the thickening agent is curcumin and carmine pigment mixed according to the mass ratio of 3: 1;

and (3) dripping the soft capsule content and the sealing layer mucilage for forming to prepare the ganoderma lucidum spore oil soft capsule, wherein the thickness of the soft capsule content wrapped by the sealing layer mucilage is 90 mu m, cooling, drying and shaping, and then sequentially coating with the binding layer mucilage and the slow release layer mucilage through fluidized bed spraying, wherein the coating thickness of the binding layer mucilage is 5 mu m, the coating thickness of the slow release layer mucilage is 60 mu m, drying, shaping and cleaning to obtain a finished product.

Example 3:

a preparation process of a ganoderma lucidum spore oil soft capsule comprises the following process steps:

s1: taking a ganoderma spore raw material, carrying out superfine grinding and wall breaking treatment, sieving and granulating to obtain ganoderma spore granules;

supercritical CO is adopted during wall breaking treatment of superfine grinding₂Carrying a ganoderma lucidum spore raw material under the pressure of 26MPa and the temperature of 10 ℃ through a nozzle, impacting a hard target material, carrying out jet flow crushing, reducing the pressure to 8Mpa, separating to obtain ganoderma lucidum spore powder, sieving to obtain the ganoderma lucidum spore powder with the fineness of 300 meshes, granulating the ganoderma lucidum spore powder into 100-mesh ganoderma lucidum spore particles through a granulating machine, and drying in vacuum;

s2: performing supercritical extraction and separation on ganoderma spore particles to obtain ganoderma spore oil, wherein the ganoderma spore oil, the ganoderma spore powder, the soybean phospholipid, the active peptide, the docosahexaenoic acid and the vitamins are mixed according to the mass ratio: 76: 8:4:1: 1.3: 0.18, mixing uniformly to prepare the content of the soft capsule;

the supercritical extraction is carried out in an extraction kettle at the pressure of 35MPa and the temperature of 42 ℃ and by supercritical CO₂The flow rate is 44Kg/h, the ultrasonic frequency is 50kHz, the ultrasonic power is 600W, and supercritical CO is carried out₂Extracting for 6h, cooling in the first-stage separation kettle to 8 deg.C under 10Mpa, cooling in the second-stage separation kettle to 8 deg.C, and separating to collect Ganoderma spore oil;

the active peptide is composed of seaweed peptide, soybean peptide and walnut peptide according to the mass ratio of 1:1:2, and the vitamin is composed of fat-soluble vitamin A, vitamin E and vitamin K according to the mass ratio of 1:1: 1;

the soft capsule content is also added with tert-butyl hydroquinone accounting for 0.03 percent of the total mass ratio and propyl gallate accounting for 0.06 percent of the total mass ratio;

s3: melting the soft capsule shell material at 75 deg.C, stirring, maintaining the temperature for 2 hr, standing, and defoaming to obtain sealing layer mucilage, binding layer mucilage and slow release layer mucilage; the soft capsule shell material of the sealing layer mucilage is hydroxypropyl methyl cellulose, a plasticizer and deionized water according to the mass ratio of 55: 18: 36, wherein the plasticizer is composed of agar and phthalate according to the mass ratio of 1: 2;

the soft capsule shell material of the bonding layer mucilage is a high molecular compound, a thickening agent sodium citrate and deionized water according to the mass ratio of 56: 14: 25, wherein the high molecular compound is guar gum, chitin and gum arabic according to the mass ratio of 1:1: 2;

the soft capsule shell material of the sustained-release layer mucilage is prepared from pullulan polysaccharide, gelatin, a plasticizer, a disintegrating agent sodium alginate, an opacifier and deionized water according to a mass ratio of 28:33: 17: 11:0.3:18, the plasticizer is glycerol and polyethylene glycol 400 according to the mass ratio of 1:2, and the opacifier is carotene and brilliant blue pigment 2: 1;

and (3) dripping the soft capsule content and the sealing layer mucilage for forming to prepare the ganoderma lucidum spore oil soft capsule, wherein the thickness of the soft capsule content wrapped by the sealing layer mucilage is 85 microns, cooling, drying and shaping, and then sequentially coating with the binding layer mucilage and the slow release layer mucilage through fluidized bed spraying, wherein the coating thickness of the binding layer mucilage is 6 microns, the coating thickness of the slow release layer mucilage is 130 microns, drying, shaping and cleaning to obtain a finished product.

The mechanism of the invention is as follows:

(1) by using supercritical CO₂The ganoderma lucidum spore powder obtained by jet crushing and pressure reduction separation is subjected to superfine crushing and wall breaking treatment on ganoderma lucidum spores, the problem of diffusion reduction caused by increase of fluid viscosity is avoided, the mutual collision and penetration effects of acceleration and formation of multi-spores are guaranteed, the ganoderma lucidum spore powder has the characteristic of no doping and easy separation, the wall breaking rate reaches over 88 percent, the wall breaking efficiency is improved, the absorption of human bodies to nutrients is promoted, efficient extraction of spore oil is facilitated, the powder is subjected to granulation mechanism to obtain 40-120-mesh ganoderma lucidum spore particles and vacuum drying after sieving with the fineness of more than or equal to 200 meshes, and the problem of extraction failure caused by pipeline blockage due to excessively fine ganoderma lucidum spore powder in extraction is avoided;

(2) optimizing supercritical extraction conditions and combining ultrasonic auxiliary supercritical CO₂Extracting, cooling in one-stage or two-stage separation kettle to 5-10 deg.C, separating under 8-10Mpa, and collecting the extractThe ganoderma lucidum spore oil is compounded with ganoderma lucidum spore powder, soybean phospholipid, active peptide, docosahexaenoic acid and vitamins, wherein the soybean phospholipid is ester consisting of glycerol, fatty acid, choline or cholelamine, has no side effect of medicaments, and has positive effect on preventing and treating diseases;

the active peptides including but not limited to balsam pear peptide, sea cucumber peptide, seaweed peptide, soybean peptide and walnut peptide have the functions of resisting virus, resisting hypertension, regulating immunity, regulating hormone, inhibiting bacteria, reducing cholesterol and the like by peptide bonds of at least more than two amino acids, the docosahexaenoic acid is favorable for promoting the cell activity, the vitamins including but not limited to fat-soluble vitamin A, vitamin D, vitamin E and vitamin K promote the metabolism of organisms and resist oxidation, and one or more of butyl hydroxy anisole, 2, 6-di-tert-butyl-4-methylphenol, tert-butyl hydroquinone and propyl gallate are added for resisting oxidation, so that the biological activity of the content of the soft capsule after compounding is higher, and the content of the soft capsule is inhibited from being oxidized and broken into small molecular chains and is crosslinked with the capsule shell;

(3) the soft capsule shell material is melted, evenly stirred and defoamed to prepare sealing layer mucilage, binding layer mucilage and slow release layer mucilage, the glue dripping molding is carried out, the binding layer mucilage and the slow release layer mucilage are sprayed on a fluidized bed, the thickness of the content of the soft capsule, which is wrapped by the sealing layer mucilage, of the ganoderma spore oil soft capsule is controlled to be 80-100 mu m, the thickness of the coating of the binding layer mucilage is 4-8 mu m, and the thickness of the coating of the slow release layer mucilage is 50-150 mu m, so that a finished product of the ganoderma spore oil soft capsule with a multilayer shell is prepared;

the soft capsule shell material of the sealing layer mucilage adopts hydroxypropyl methyl cellulose, has the characteristics of surface activity, high transparency, stable performance, film forming property, dispersibility and cohesiveness, and comprises but is not limited to plasticizers of glycerol, agar, polyethylene glycol 400 and phthalate for increasing the film forming property, the soft capsule content is dripped and wrapped to form a sealing layer rubber, the sealing layer rubber is sealed under the action of gravity acceleration reduction to form a soft capsule, the drying and the shaping are carried out, the autoxidation or the crosslinking action of the soft capsule content and amino acid residues in gelatin is blocked, and the problem that the degradation and the inactivation of the capsule content caused by the denaturation, the dissolution time extension and the crosslinking of the capsule shell in the storage process influence the stability and the nutritional value is avoided;

the soft capsule shell material of the bonding layer mucilage adopts thickening agents including but not limited to guar gum, chitin, pectin, gum arabic macromolecular compounds and thickening agents including but not limited to propylene glycol alginate and sodium citrate, wherein the guar gum is nonionic galactomannan extracted from endosperm of guar bean of leguminous plants, the chitin is structurally-homologous polysaccharide formed by polymerizing N-acetylglucosamine through beta connection, linear polygalacturonic acid and poly L-rhamnogalacturonic acid of which the pectin hydroxyl groups are esterified by methyl to different degrees, the gum arabic comprises aldose, galactose, glucuronic acid and the like, and is emulsified and stabilized with the propylene glycol alginate and the sodium citrate, so that the bonding performance of the sealing layer mucilage layer and the sealing layer mucilage layer is improved, and layering is avoided;

the sustained-release layer mucilage soft capsule shell material adopts pullulan polysaccharide, gelatin, plasticizer, disintegrating agent and opacifier, wherein the pullulan polysaccharide is extracellular water-soluble mucopolysaccharide which is produced by fermentation of aureobasidium pullulans and is similar to glucan and xanthan gum, has strong film forming property, gas barrier property, plasticity and viscosity, and plays a role in resisting oxidation, the disintegrating agent starch and sodium alginate are quickly cracked into fine particles in gastrointestinal fluid and are quickly dissolved and absorbed, the opacifier reduces ultraviolet effect, inhibits autoxidation or crosslinking in the storage process, and the release controllability of capsule contents is improved;

the ganoderma lucidum spore oil soft capsules prepared in examples 1 to 3 and the commercial ganoderma lucidum spore oil soft capsules (xianzhuo brand) were compared for comparative detection, and the results were as follows:

sequence number/item	Acid value/(mg.g-1)	Peroxide number (%)	Total triterpene (%)	Polysaccharide (%)	Disintegration time (min)
						Detection standard	GB/T15689-1995	GB/T5009.37-2003	Landmark DB44	ZL-SOP-TY005	Appendix XA of the 2000 pharmacopoeia
Example 1	74.31	0.083	11.23	2.11	15.3
						Example 2	64.14	0.067	12.55	3.54	11.35
Example 3	54.87	0.047	10.57	4.35	20.8
						Comparative example	36.47	0.054	2.54	1.31	10.34

The finished product of the ganoderma spore oil soft capsule can be applied to preparing anti-tumor food, health-care food or medicine, the release is controllable, and the problems that the disintegration time of the soft capsule is prolonged and the inner wall of the capsule shell and the ganoderma seed oil contained in the capsule shell is contacted into a membranous substance to influence the stability and the quality of the product in the storage period are avoided.

The above description is only for the preferred embodiment of the present invention, but the scope of the present invention is not limited thereto, and any changes or substitutions that can be easily conceived by those skilled in the art within the technical scope of the present invention are also included in the scope of the present invention. Therefore, the protection scope of the present invention shall be subject to the protection scope of the claims.