阅读说明：本技术 一种软组织填充剂及其应用 (Soft tissue filler and application thereof ) 是由 白晋 于 2018-08-27 设计创作，主要内容包括：本发明公开了一种软组织填充剂,所述软组织填充剂的原料包括：胶原蛋白、脂肪干细胞、细胞生长因子和交联剂。本发明采用胶原蛋白和脂肪干细胞介导移植隆胸,脂肪干细胞能明显提高移植脂肪细胞的成活率,脂肪干细胞分化的组织与胶原蛋白相结合,搭配使用促进细胞生长的细胞生长因子,能够进一步地抑制移植脂肪的不良吸收、感染、坏死,促进移植脂肪组织的血管再生,提高移植脂肪的成活率,有利于乳房的塑形。本发明不会产生异物反应,安全可靠,具有获取方便、来源丰富、填充效果好以及创伤小等优势。(The invention discloses a soft tissue filler, which comprises the following raw materials: collagen, adipose-derived stem cells, cell growth factors and cross-linking agents. The invention adopts collagen and adipose-derived stem cells to mediate transplantation and breast augmentation, the adipose-derived stem cells can obviously improve the survival rate of the transplanted adipose-derived cells, the differentiated tissues of the adipose-derived stem cells are combined with the collagen, and cell growth factors for promoting cell growth are matched for use, so that the poor absorption, infection and necrosis of the transplanted adipose can be further inhibited, the revascularization of the transplanted adipose tissue is promoted, the survival rate of the transplanted adipose is improved, and the breast augmentation can be facilitated. The invention does not generate foreign body reaction, is safe and reliable, and has the advantages of convenient acquisition, rich sources, good filling effect, small wound and the like.)

1. A soft tissue filler, characterized in that the raw materials of the soft tissue filler comprise: collagen, adipose-derived stem cells, cell growth factors and cross-linking agents.

2. The soft tissue filler according to claim 1, comprising the following raw materials in parts by weight: 50-80 parts of collagen, 20-5 parts of adipose-derived stem cells, 10-30 parts of cell growth factors and 1-20 parts of cross-linking agents.

3. The soft tissue filler according to claim 1 or 2, characterized in that the preparation method of the soft tissue filler comprises: mixing collagen, adipose-derived stem cells, cell growth factor and cross-linking agent, and storing at 4 deg.C under sterile condition.

4. The soft tissue filler according to claim 1 or 2, wherein the collagen is prepared by a method comprising:

separating blood to obtain a plasma layer, a leucocyte layer and a red blood cell layer of the blood, mixing the plasma layer and the leucocyte layer of the blood to obtain a mixture, and purifying the mixture to obtain collagen;

the volume ratio of the plasma layer to the leucocyte layer is 1: 1.

5. The soft tissue filler of claim 1 or 2, wherein the adipose stem cells are prepared by a method comprising:

mixing adipose tissues with physiological saline, centrifuging to obtain pure adipose tissues, performing enzymolysis on the adipose tissues to obtain primary adipose stem cells, performing subculture on the adipose stem cells to obtain third-generation adipose single cells, and obtaining the adipose stem cells when the fusion rate of the third-generation adipose stem cells is more than or equal to 80%.

6. The soft tissue bulking agent of claim 1 or 2, wherein the cellular growth factor comprises one or more of a fibroblast growth factor, an epidermal growth factor and an insulin-like growth factor.

7. The soft tissue filler of claim 1 or 2, wherein the cross-linking agent comprises one or both of 1, 4-butanediol glycidyl ether and polyethylene glycol diglycidyl ether.

8. Use of a soft tissue filler according to any one of claims 1 to 7 in the manufacture of a medicament for filling soft tissue.

9. The use of claim 8, wherein the soft tissue comprises soft tissue of the chest.

Technical Field

The invention relates to the technical field of biological preparations, in particular to a preparation based on autologous tissues and application thereof.

Background

Breast augmentation, also known as breast augmentation or breast enlargement, is an operation for enlarging the breast volume, enlarging and balancing the shape, improving the body form of women and recovering the special beauty of women by implanting medical materials or transplanting self tissues. Since the early 80 s in the 19 th century, breast augmentation by liquid paraffin injection and breast implantation of silicone oil and silica gel prostheses have been carried out successively, but medical seekers and doctors have been confused by the problems of related complications including contracture, deformation, hardening, leakage, unnatural hand feeling and the like. Therefore, the search for safe, effective, non-toxic, long-lasting tissue substitutes has become a key to the development of breast augmentation.

The autologous fat is prepared by sucking redundant subcutaneous fat cells from certain parts of a human body, purifying the sucked mixture, and injecting a medicament to obtain the composite fat particles. When people realize that autologous fat is likely to become a material for breast enlargement, the autologous fat is immediately popular with doctors and operators and has great expectations. The chest augmentation by autologous fat injection is in clinic from the end of the 19 th century, and because of natural hand feeling, small wound and continuous improvement and maturity of technology, the number of patients who receive operation treatment is increasing. However, whether the breast is raised by autologous fat injection is related to the survival rate of fat cells, absorption and other problems exist after fat tissue injection, so that multiple operations are often required, and complications such as liquefaction infection and the like are difficult.

In view of this, the present invention is set forth.

Disclosure of Invention

In order to solve the problems of the prior art, the invention aims to provide a preparation based on autologous tissue and application thereof.

In order to achieve the above object, the present invention provides a soft tissue filler, which comprises the following raw materials: collagen, adipose-derived stem cells, cell growth factors and cross-linking agents.

Preferably, the soft tissue filler comprises the following raw materials in parts by weight: 50-80 parts of collagen, 20-5 parts of adipose-derived stem cells, 10-30 parts of cell growth factors and 1-20 parts of cross-linking agents.

Preferably, the preparation method of the soft tissue filler comprises the following steps: mixing collagen, adipose-derived stem cells, cell growth factor and cross-linking agent, and storing at 4 deg.C under sterile condition.

Preferably, the preparation method of the collagen comprises the following steps:

separating blood to obtain a plasma layer, a leucocyte layer and a red blood cell layer of the blood, mixing the plasma layer and the leucocyte layer of the blood to obtain a mixture, and purifying the mixture to obtain collagen;

the volume ratio of the plasma layer to the leucocyte layer is 1: 1.

Preferably, the method for preparing the adipose-derived stem cells comprises the following steps:

mixing adipose tissues with physiological saline, centrifuging to obtain pure adipose tissues, performing enzymolysis on the adipose tissues to obtain primary adipose stem cells, performing subculture on the adipose stem cells to obtain third-generation adipose single cells, and obtaining the adipose stem cells when the fusion rate of the third-generation adipose stem cells is more than or equal to 80%.

Preferably, the cell growth factor comprises one or more of fibroblast growth factor, epidermal growth factor and insulin-like growth factor.

Preferably, the crosslinking agent comprises one or both of 1, 4-butanediol glycidyl ether and polyethylene glycol diglycidyl ether.

Preferably, the soft tissue filler dosage form comprises an injection.

The invention also provides a preparation method of the soft tissue filler with collagen and adipose-derived stem cells as raw materials, which comprises the following steps:

step one, preparation of autologous collagen

Blood is collected by a human body through blood collection, the blood enters a whole blood separator after being centrifuged for the first time, secondary centrifugation is carried out in the whole blood separator, the whole blood centrifugation is divided into three layers, namely a plasma layer, a white membrane layer and a red blood cell layer from top to bottom, the liquids of the plasma layer, the white membrane layer and the red blood cell layer are respectively led into different storage units through guide tubes to be stored separately, a proper amount of the liquids of the plasma layer and the white membrane layer are taken out from the storage units to be mixed in proportion, and the liquids are subjected to primary purification through a chromatography unit and further purified through a diafiltration unit to obtain the required autologous collagen;

step two, preparation of autologous adipose-derived stem cells

(1) Fat stem cell separation: obtaining autologous adipose tissues located in the abdomen, adding normal saline, mixing, centrifuging at the rotating speed of 1200-1800 r/min for 10-20 min, removing residual blood cells and tissue fragments, obtaining adipose tissues, and adding the mixture into the adipose tissues according to the volume ratio of 1: 2-3, adding a collagenase I solution with the mass concentration of 0.075-0.1%, digesting in a thermostatic water bath at 37 ℃ for 30-60min, centrifuging at the rotating speed of 1000-1500 r/min for 2-6 min, and removing upper adipose tissues and lower suspension to obtain adipose-derived stem cell sediment;

(2) culturing the adipose-derived stem cells: adding the obtained adipose-derived stem cell precipitate into DMEM medium for resuspension, and placing at 37 deg.C and 5% CO₂Culturing, carrying out passage, stopping until the 3 rd generation, and collecting cells for use or freezing when the fusion degree of the adipose-derived mesenchymal stem cells reaches more than 80%;

step three, preparation

And (3) uniformly mixing the autologous collagen prepared in the step one and the autologous adipose-derived stem cells prepared in the step two, and placing the mixture at 4 ℃ for aseptic storage.

Preferably, the preparation also comprises cell growth factors for promoting cell growth, and the preparation comprises 50-80% of autologous collagen, 20-50% of autologous adipose-derived stem cells and 10-30% of cell growth factors for promoting cell growth in percentage by weight.

Preferably, the cell growth factor comprises one or more of fibroblast growth factor, epidermal growth factor and insulin-like growth factor.

Preferably, the preparation also comprises 1-20 wt% of a cross-linking agent.

Preferably, the crosslinking agent is selected from one or two of 1, 4-butanediol glycidyl ether and polyethylene glycol diglycidyl ether.

The invention also provides application of the soft tissue filler in preparing a medicament for filling soft tissues.

Preferably, the soft tissue comprises soft tissue of the chest.

The breasts are composed of connective tissues and adipose tissues, and the stiff and full breasts are supported by the connective tissues to a great extent. The collagen is the main component of connective tissue, and in the connective tissue, the collagen and the polysaccharide protein are often interwoven into a net structure to generate certain mechanical strength, which is a material basis for supporting the body curve and showing the stiff and straight posture. Adipose-derived stem cells have a multi-differentiation potential and are genetically stable, and are ideal seed cells for tissue engineering and regenerative medicine.

The autologous collagen of the embodiment of the invention is prepared from autologous collagen which is extracted from human venous blood and is rich in a large amount of platelets and collagen, the blood is divided into three layers by centrifugation, the plasma layer mainly comprises plasma, water, protein, salts, various ions and the like, the white membrane layer mainly comprises a platelet-rich area, a lymphocyte-rich area, a monocyte-rich area and a granulocyte-rich area, the red cell layer can be simply divided into young red cells and normal red cells, the mixed solution of the extracted plasma layer and the white membrane layer contains a large amount of collagen and is rich in platelets and various growth factors, and after entering the dermis, the autologous collagen, elastic fibers, colloid and the like can be stimulated to generate, so that the tissue regeneration is promoted.

The adipose-derived stem cells are stem cells with multidirectional differentiation potential separated from adipose tissues, and researches show that the adipose-derived stem cells can be stably proliferated in vitro and have low decline rate, and meanwhile, the adipose-derived stem cells have the advantages of easiness in material obtaining, capability of obtaining a large amount of stem cells from a small amount of tissues, suitability for large-scale culture, small damage to organisms and the like, and have the characteristics of wide sources, large in vivo reserve and suitability for autografting. Meanwhile, the adipose-derived stem cells can secrete various cell growth factors in the growth and proliferation process, such as fibroblast growth factor, keratinocyte growth factor and the like, and have the effects of promoting cell renewal, promoting the healing of damaged cell tissues, increasing cell activity and skin immunity, beautifying, resisting aging and the like.

The embodiment of the invention has the following advantages:

1. according to the embodiment of the invention, autologous collagen and autologous adipose-derived stem cell-mediated transplantation breast augmentation are adopted, autologous adipose-derived stem cells can obviously improve the survival rate of transplanted adipose cells, tissues differentiated from autologous adipose-derived stem cells are combined with autologous collagen, and cell growth factors promoting cell growth are further matched for use, so that the absorption, infection and necrosis of transplanted fat can be effectively inhibited, the vascular regeneration of the transplanted adipose tissues is promoted, the survival rate of the transplanted fat is high, and the breast formation effect is good.

2. The embodiment of the invention utilizes the effective components of the autologous tissue to fill the tissue, and the extract is from the autologous tissue of the patient, does not generate foreign body reaction, is safe and reliable, and has the advantages of convenient acquisition, rich sources, good filling effect, small wound and the like.

Detailed Description

The following examples are intended to illustrate the invention but are not intended to limit the scope of the invention.

Example 1

The preparation method of the injection based on autologous tissue of the embodiment comprises the following steps:

step one, preparation of autologous collagen

Blood is collected by a human body through blood collection, the blood enters a whole blood separator after being centrifuged for the first time, secondary centrifugation is carried out in the whole blood separator, the whole blood centrifugation is divided into three layers, namely a plasma layer, a white membrane layer and a red blood cell layer from top to bottom, the liquids of the plasma layer, the white membrane layer and the red blood cell layer are respectively led into different storage units through guide tubes to be stored separately, a proper amount of the liquids of the plasma layer and the white membrane layer are taken out from the storage units to be mixed according to the ratio of 1:1, and the liquids are subjected to primary purification through a chromatography unit and further purified through a diafiltration unit to obtain the required autologous collagen;

step two, preparation of autologous adipose-derived stem cells

(1) Fat stem cell separation: obtaining autologous adipose tissues located in the abdomen, adding physiological saline, mixing, centrifuging at a rotating speed of 1600r/min for 15min, removing residual blood cells and tissue fragments, obtaining adipose tissues, and adding the mixture into the adipose tissues according to a volume ratio of 1: 2, adding a collagenase I solution with the mass concentration of 0.075%, digesting in a thermostatic water bath at 37 ℃ for 60min, centrifuging at the rotating speed of 1200r/min for 4min, and removing upper-layer adipose tissues and lower-layer suspension to obtain adipose-derived stem cell sediment;

(2) culturing the adipose-derived stem cells: adding the obtained adipose-derived stem cell precipitate into DMEM medium for resuspension, and placing at 37 deg.C and 5% CO₂Culturing, carrying out passage, stopping until the 3 rd generation, and collecting cells for use or freezing when the fusion degree of the adipose-derived mesenchymal stem cells reaches more than 80%;

step three, preparation

According to the weight percentage, 60 percent of autologous collagen, 25 percent of autologous adipose-derived stem cells, 15 percent of fibroblast growth factor and 5 percent of 1, 4-butanediol glycidyl ether are uniformly mixed to prepare a preparation, and the preparation is placed at 4 ℃ for aseptic storage.

Example 2

An injection based on autologous tissue, the preparation method comprises:

step one, preparation of autologous collagen

Blood is collected by a human body through blood collection, the blood enters a whole blood separator after being centrifuged for the first time, secondary centrifugation is carried out in the whole blood separator, the whole blood centrifugation is divided into three layers, namely a plasma layer, a white membrane layer and a red blood cell layer from top to bottom, the liquids of the plasma layer, the white membrane layer and the red blood cell layer are respectively led into different storage units through guide tubes to be stored separately, a proper amount of the liquids of the plasma layer and the white membrane layer are taken out from the storage units to be mixed according to the ratio of 1:1, and the liquids are subjected to primary purification through a chromatography unit and further purified through a diafiltration unit to obtain the required autologous collagen;

step two, preparation of autologous adipose-derived stem cells

(1) Fat stem cell separation: obtaining autologous adipose tissues located in the abdomen, adding physiological saline, mixing, centrifuging at the rotating speed of 1200r/min for 20min, removing residual blood cells and tissue fragments, obtaining adipose tissues, and adding the mixture into the adipose tissues according to the volume ratio of 1: 2 adding a collagenase I solution with the mass concentration of 0.1%, digesting in a thermostatic water bath at 37 ℃ for 30min, centrifuging at the rotating speed of 1500r/min for 2min, and removing upper adipose tissues and lower suspension to obtain adipose-derived stem cell sediment;

(2) culturing the adipose-derived stem cells: adding the obtained adipose-derived stem cell precipitate into DMEM medium for resuspension, and placing at 37 deg.C and 5% CO₂Culturing, carrying out passage, stopping until the 3 rd generation, and collecting cells for use or freezing when the fusion degree of the adipose-derived mesenchymal stem cells reaches more than 80%;

step three, preparation

According to the weight percentage, 55 percent of autologous collagen, 20 percent of autologous adipose-derived stem cells, 20 percent of epidermal growth factor and 5 percent of polyethylene glycol diglyceride are uniformly mixed to prepare a preparation, and the preparation is placed at 4 ℃ for aseptic storage.

Example 3

An injection based on autologous tissue, the preparation method comprises:

step one, preparation of autologous collagen

Blood is collected by a human body through blood collection, the blood enters a whole blood separator after being centrifuged for the first time, secondary centrifugation is carried out in the whole blood separator, the whole blood centrifugation is divided into three layers, namely a plasma layer, a white membrane layer and a red blood cell layer from top to bottom, the liquids of the plasma layer, the white membrane layer and the red blood cell layer are respectively led into different storage units through guide tubes to be stored separately, a proper amount of the liquids of the plasma layer and the white membrane layer are taken out from the storage units to be mixed according to the ratio of 1:1, and the liquids are subjected to primary purification through a chromatography unit and further purified through a diafiltration unit to obtain the required autologous collagen;

step two, preparation of autologous adipose-derived stem cells

(1) Fat stem cell separation: obtaining autologous adipose tissues located in the abdomen, adding physiological saline, mixing, centrifuging at the rotating speed of 1200r/min for 18min, removing residual blood cells and tissue fragments, obtaining adipose tissues, and adding the mixture into the adipose tissues according to the volume ratio of 1: 3 adding a collagenase I solution with the mass concentration of 0.075%, digesting in a thermostatic water bath at 37 ℃ for 45min, centrifuging at the rotating speed of 1200r/min for 6min, and removing upper-layer adipose tissues and lower-layer suspension to obtain adipose-derived stem cell sediment;

(2) culturing the adipose-derived stem cells: adding the obtained adipose-derived stem cell precipitate into DMEM medium for resuspension, and placing at 37 deg.C and 5% CO₂Culturing, carrying out passage, stopping until the 3 rd generation, and collecting cells for use or freezing when the fusion degree of the adipose-derived mesenchymal stem cells reaches more than 80%;

step three, preparation

According to the weight percentage, 60 percent of autologous collagen, 20 percent of autologous adipose-derived stem cells, 17 percent of epidermal growth factor and 3 percent of 1, 4-butanediol glycidyl ether are uniformly mixed to prepare a preparation, and the preparation is placed at 4 ℃ for aseptic storage.

Example 4

An injection based on autologous tissue, the preparation method comprises:

step one, preparation of autologous collagen

Blood is collected by a human body through blood collection, the blood enters a whole blood separator after being centrifuged for the first time, secondary centrifugation is carried out in the whole blood separator, the whole blood centrifugation is divided into three layers, namely a plasma layer, a white membrane layer and a red blood cell layer from top to bottom, the liquids of the plasma layer, the white membrane layer and the red blood cell layer are respectively led into different storage units through guide tubes to be stored separately, a proper amount of the liquids of the plasma layer and the white membrane layer are taken out from the storage units to be mixed according to the ratio of 1:1, and the liquids are subjected to primary purification through a chromatography unit and further purified through a diafiltration unit to obtain the required autologous collagen;

step two, preparation of autologous adipose-derived stem cells

(1) Fat stem cell separation: obtaining autologous adipose tissues located in the abdomen, adding physiological saline, mixing, centrifuging at the rotating speed of 1200r/min for 20min, removing residual blood cells and tissue fragments, obtaining adipose tissues, and adding the mixture into the adipose tissues according to the volume ratio of 1: 2 adding a collagenase I solution with the mass concentration of 0.09%, digesting in a thermostatic water bath at 37 ℃ for 35min, centrifuging at the rotating speed of 1200r/min for 3min, and removing upper adipose tissues and lower suspension to obtain adipose-derived stem cell sediment;

(2) culturing the adipose-derived stem cells: adding the obtained adipose-derived stem cell precipitate into DMEM medium for resuspension, and placing at 37 deg.C and 5% CO₂Culturing, carrying out passage, stopping until the 3 rd generation, and collecting cells for use or freezing when the fusion degree of the adipose-derived mesenchymal stem cells reaches more than 80%;

step three, preparation

According to the weight percentage, 70 percent of autologous collagen, 10 percent of autologous adipose-derived stem cells, 12 percent of insulin-like growth factors and 8 percent of polyethylene glycol diglyceride are uniformly mixed to prepare a preparation, and the preparation is placed at 4 ℃ for aseptic storage.

Example 5

An injection based on autologous tissue, the preparation method comprises:

step one, preparation of autologous collagen

Blood is collected by a human body through blood collection, the blood enters a whole blood separator after being centrifuged for the first time, secondary centrifugation is carried out in the whole blood separator, the whole blood centrifugation is divided into three layers, namely a plasma layer, a white membrane layer and a red blood cell layer from top to bottom, the liquids of the plasma layer, the white membrane layer and the red blood cell layer are respectively led into different storage units through guide tubes to be stored separately, a proper amount of the liquids of the plasma layer and the white membrane layer are taken out from the storage units to be mixed according to the ratio of 1:1, and the liquids are subjected to primary purification through a chromatography unit and further purified through a diafiltration unit to obtain the required autologous collagen;

step two, preparation of autologous adipose-derived stem cells

(1) Fat stem cell separation: obtaining autologous adipose tissues located in the abdomen, adding physiological saline, mixing, centrifuging at a rotating speed of 1500r/min for 20min, removing residual blood cells and tissue fragments, obtaining adipose tissues, and adding the mixture into the adipose tissues according to a volume ratio of 1: 2 adding a collagenase I solution with the mass concentration of 0.1%, digesting in a thermostatic water bath at 37 ℃ for 30min, centrifuging at the rotating speed of 1000r/min for 6min, and removing upper adipose tissues and lower suspension to obtain adipose-derived stem cell sediment;

(2) culturing the adipose-derived stem cells: adding the obtained adipose-derived stem cell precipitate into DMEM medium for resuspension, and placing at 37 deg.C and 5% CO₂Culturing, subculturing toTerminating the 3 rd generation, and collecting the cells for use or freezing when the fusion degree of the adipose-derived mesenchymal stem cells reaches more than 80%;

step three, preparation

According to the weight percentage, 50 percent of autologous collagen, 30 percent of autologous adipose-derived stem cells, 10 percent of fibroblast growth factor and 10 percent of 1, 4-butanediol glycidyl ether are uniformly mixed to prepare a preparation, and the preparation is placed at 4 ℃ for aseptic storage.

Test example

In order to demonstrate that the preparation of the present invention made of autologous tissue has a good breast enhancement effect, the present invention conducted the following therapeutic effect test.

1. General data

60 women who voluntarily receive breast augmentation in the mechanism in 2 months-2017 in 2015 are selected as study objects, the study objects are 20-45 years old, the health condition is good, other systemic diseases do not exist, and informed consent is signed before breast augmentation. Inclusion criteria were: first, primary breast dysplasia and flat chest. ② spontaneous breast atrophy after pregnancy. ③ breast dysplasia after mammary tissue lesion or trauma. Fourthly, the breasts are not symmetrical or have slight ptosis. The patients are brought into the experiment voluntarily and signed an informed consent. Exclusion criteria: the psychological preparation is insufficient. ② those with inflammation of breast tissue or skin inflammation near the surgical incision. ③ those suffering from schizophrenia or psychosis. And those with pathological changes in the vital organs such as heart, liver and kidney. Patients with immune system or hematopoietic system diseases. Sixthly, the breast cancer has recurrence or metastasis tendency after operation. The patients with breast augmentation were randomly divided into trial 1-5 groups and control group, each group was 10 patients, and the two groups of patients were relatively indistinct in general data and had no statistical significance (P > 0.05).

2. Method of treatment

Test groups: the injections prepared in examples 1 to 5 of the present invention were used in test groups 1 to 5, respectively. The injection prepared by the embodiment of the invention is injected according to the multi-tunnel multi-point of the subcutaneous layer and the mammary gland lower layer according to the designed injection point, and after the injection is finished, the fat is uniformly distributed by massage.

Control group: injecting the prepared autologous fat according to the designed injection points and the multi-tunnel multi-point injection of subcutaneous layer and mammary gland lower layer, and massaging to make the fat distributed uniformly after injection.

3. Criteria for therapeutic effect

The effect is shown: the positions and the sizes of different positions are symmetrical and harmonious; the hand feeling is natural and the dynamic sense is vivid; the feeling is sensitive.

The method has the following advantages: the position and the size under the vertical position are symmetrical and harmonious; the position and size symmetry and harmony are slightly poor in the lying position; natural hand feeling and slightly poor dynamic sense; the feeling is sensitive.

And (4) invalidation: the symmetry of the position and the size under various body positions is poor; unnatural hand feeling and poor dynamic sense; the sensation is not sensitive.

4. Results

The postoperative follow-up is carried out for 3-6 months, and the comparison result of the treatment effects of the postoperative follow-up and the postoperative follow-up is shown in table 1.

TABLE 1

Group of	Show effect	Is effective	Invalidation	Total effective rate
					Test group 1	8	1	1	90％
Test group 2	7	2	1	90％
					Test group 3	7	3	0	100％
Test group 4	8	1	1	90％
					Test group 5	8	2	0	100％
Control group	2	4	4	60％

The results show that: the total effective rate of the test groups 1-5 reaches more than 90%, the total effective rate is obviously superior to that of the control group by 60%, and the difference has statistical significance. The embodiment of the invention can effectively inhibit the absorption, infection and necrosis of the transplanted fat and promote the regeneration of blood vessels of the transplanted fat tissue, and has high survival rate of the transplanted fat and good breast forming effect.

Although the invention has been described in detail above with reference to a general description and specific examples, it will be apparent to one skilled in the art that modifications or improvements may be made thereto based on the invention. Accordingly, such modifications and improvements are intended to be within the scope of the invention as claimed.