阅读说明：本技术 昆虫蛋白源生物饲料的制备方法 (Preparation method of insect protein source biological feed ) 是由 冯金山 于 2021-01-22 设计创作，主要内容包括：本发明公开了一种昆虫蛋白源生物饲料的制备方法,包括以下步骤：S1,按重量比计选择以下第一原料：菌种原液10～15份、红糖50～60份、富硒酵母20～30份、短链脂肪酸10～15份、谷氨酸钠5～10份；S2,将各原料加入按重量比计5～15倍的水中形成混合液,将混合液静止；S3,按重量比计选择以下第二原料：酵素渣20～30份、黄粉虫粪沙20～30份、大麦虫粪沙20～30份、松针粉5～10份、玉米粉10份～15份,将各第二原料混合形成混合料；S4,将静止后的混合液和混合物按重量计比为1～3混合均匀后进行发酵,其中,发酵的工艺参数为：温度为23℃～35℃,空气湿度为65％～75％,发酵时间为3天～5天。本发明应用于生物饲料技术领域。(The invention discloses a preparation method of insect protein source biological feed, which comprises the following steps: s1, selecting the following first raw materials in percentage by weight: 10-15 parts of strain stock solution, 50-60 parts of brown sugar, 20-30 parts of selenium-enriched yeast, 10-15 parts of short-chain fatty acid and 5-10 parts of sodium glutamate; s2, adding the raw materials into water in an amount which is 5-15 times the weight of the raw materials to form a mixed solution, and standing the mixed solution; s3, selecting the following second raw materials in percentage by weight: 20-30 parts of enzyme residues, 20-30 parts of tenebrio molitor dung sand, 20-30 parts of barley pest dung sand, 5-10 parts of pine needle powder and 10-15 parts of corn powder, and mixing the second raw materials to form a mixture; s4, uniformly mixing the static mixed liquid and the mixture according to the weight ratio of 1-3, and fermenting, wherein the fermentation process parameters are as follows: the temperature is 23-35 ℃, the air humidity is 65-75%, and the fermentation time is 3-5 days. The invention is applied to the technical field of biological feed.)

1. A preparation method of insect protein source biological feed is characterized by comprising the following steps:

s1, selecting the following first raw materials in percentage by weight: 10-15 parts of strain stock solution, 50-60 parts of brown sugar, 20-30 parts of selenium-enriched yeast, 10-15 parts of short-chain fatty acid and 5-10 parts of sodium glutamate;

s2, adding the raw materials into water which is 5-15 times the weight ratio of the raw materials to form a mixed solution, and standing the mixed solution in an environment with the temperature of 28-30 ℃ and the air humidity of 65-75%;

s3, selecting the following second raw materials in percentage by weight: 20-30 parts of enzyme residues, 20-30 parts of tenebrio molitor dung sand, 20-30 parts of barley pest dung sand, 5-10 parts of pine needle powder and 10-15 parts of corn powder, and mixing the second raw materials to form a mixture;

s4, uniformly mixing the static mixed liquid and the mixture according to the weight ratio of 1-3, and fermenting, wherein the fermentation process parameters are as follows: the temperature is 23-35 ℃, the air humidity is 65-75%, and the fermentation time is 3-5 days.

2. The method for preparing the insect protein-derived biological feed according to claim 1, wherein in step S1, the content of organic selenium in the selenium-enriched yeast is 2000-2400 mg/kg.

3. The method for preparing an insect protein-derived biological feed according to claim 1, wherein in step S1, the short-chain fatty acid and the sodium glutamate are food-grade.

4. The method for preparing an insect protein-derived biological feed according to claim 1, wherein in step S1, the strain in the strain stock solution is yeast or lactic acid bacteria.

5. The method of claim 1, wherein in step S2, the raw materials are added to 10 times the weight of water to form a mixture.

6. The method for preparing an insect protein-derived biological feed according to claim 1, wherein the mixed solution is allowed to stand for 1 hour in an environment having a temperature of 28 to 30 ℃ and an air humidity of 65 to 75% in step S2.

7. The method for preparing the insect protein-derived biological feed according to claim 1, wherein the method for preparing the ferment residues in step S3 comprises the following steps:

grinding one or more of hermetia illucens, yellow mealworms, barley worms and earthworms into worm pulp, mixing the worm pulp, the biological enzyme and the fermentation strain for fermentation, and obtaining worm pulp enzyme and worm pulp enzyme residues after fermentation.

8. The method for preparing an insect protein-derived biological feed according to claim 1, wherein in step S4, the mixture after standing and the mixture are mixed uniformly in a weight ratio of 1 and then placed in a fermentation vat for fermentation.

9. The method for preparing an insect protein-derived biological feed according to any one of claims 1 to 8, wherein the fermentation in step S4 produces sweet smell and white hyphae, which indicates that the feed is fermented into a finished product, and the finished product is sequentially detected and packaged.

10. The method for preparing a biological feed derived from insect proteins as claimed in claim 9, wherein in step S4, the finished feed is packaged by using a breathing bag with a breathing valve, and the breathing bag is covered with a fiber bag.

Technical Field

The invention relates to the technical field of biological feeds, in particular to a preparation method of an insect protein source biological feed.

Background

In the prior art, the traditional biological feed has high antibiotic content, single nutrient content and high preparation cost.

Disclosure of Invention

Technical problem to be solved

A method for preparing insect protein source biological feed, which solves the technical problems of high antibiotic content, single nutrient content and high preparation cost.

(II) technical scheme

In order to solve the technical problems, the invention provides a preparation method of insect protein source biological feed, which comprises the following steps:

s1, selecting the following first raw materials in percentage by weight: 10-15 parts of strain stock solution, 50-60 parts of brown sugar, 20-30 parts of selenium-enriched yeast, 10-15 parts of short-chain fatty acid and 5-10 parts of sodium glutamate;

s2, adding the raw materials into water which is 5-15 times the weight ratio of the raw materials to form a mixed solution, and standing the mixed solution in an environment with the temperature of 28-30 ℃ and the air humidity of 65-75%;

s3, selecting the following second raw materials in percentage by weight: 20-30 parts of enzyme residues, 20-30 parts of tenebrio molitor dung sand, 20-30 parts of barley pest dung sand, 5-10 parts of pine needle powder and 10-15 parts of corn powder, and mixing the second raw materials to form a mixture;

s4, uniformly mixing the static mixed liquid and the mixture according to the weight ratio of 1-3, and fermenting, wherein the fermentation process parameters are as follows: the temperature is 23-35 ℃, the air humidity is 65-75%, and the fermentation time is 3-5 days.

In a further improvement, in step S1, the content of organic selenium in the selenium-enriched yeast is 2000-2400 mg/kg.

In a further improvement, in step S1, the short chain fatty acid and the sodium glutamate are respectively food grade.

In a further improvement, in step S1, the strain in the strain stock solution is yeast or lactic acid bacteria.

In a further modification, in step S2, each raw material is added to 10 times by weight of water to form a mixed solution.

In a further modification, in step S2, the mixed solution is allowed to stand for 1 hour in an environment with a temperature of 28 to 30 ℃ and an air humidity of 65 to 75%.

In a further improvement, in step S3, the method for making the ferment residue includes the following steps:

grinding one or more of hermetia illucens, yellow mealworms, barley worms and earthworms into worm pulp, mixing the worm pulp, the biological enzyme and the fermentation strain for fermentation, and obtaining worm pulp enzyme and worm pulp enzyme residues after fermentation.

In a further improvement, in step S4, the mixture after standing and the mixture are mixed uniformly according to the weight ratio of 1, and then the mixture is put into a fermentation vat for fermentation.

In a further improvement, in step S4, when sweet smell and white hypha are generated by fermentation, the fermented hypha is a feed finished product, and then the feed finished product is sequentially detected and packaged.

In a further improvement, in step S4, during the sub-packaging, the finished feed is packaged by using a breathing bag with a breathing valve, and a fiber bag is sleeved outside the breathing bag.

(III) advantageous effects

The preparation method of the insect protein source biological feed can fully and reasonably utilize the enzyme residues, and can reduce the manufacturing cost of the insect protein source biological feed. The ferment residues are rich in micromolecular peptides, antibacterial peptides, chitin, various trace elements, beneficial bacteria, enzyme preparations, animal and plant growth factors, vitamins, amino acids and the like which are rich in ferment, so that the prepared insect protein source biological feed is balanced and comprehensive in nutrition. A large amount of active insect antibacterial small peptides and probiotic bacteria are reserved in the enzyme residues, and the derivatives of the enzyme residues also contain a large amount of immune factors, so that the immunity of the organism can be greatly improved, the morbidity of cultured animals can be well prevented in the culture of the animals, and the survival rate of the animals is greatly improved.

The selenium-enriched yeast is rich in organic selenium, so that the organic selenium of animals can be improved, and the production of green pollution-free products is realized by using the organic selenium in the feed instead of antibiotics. And secondly, the short-chain fatty acid, pine needle powder and the like capable of improving fiber and fat between fleshy muscles are provided by the selenium-enriched yeast rich in organic selenium, so that a safe selenium source product without toxic and side effects is provided for enterprises breeding high-end selenium-enriched agricultural products, the healthy growth of animals is promoted, and a reliable choice is provided for the enterprises to produce high-quality selenium-enriched agricultural products integrating health and delicious taste.

The insect source biological feed can greatly improve the concentration and the activity of probiotic groups by adding the yellow mealworm excrement sand and the barley worm excrement sand, and the yellow mealworm excrement sand and the barley worm excrement sand are not only high in protein content but also belong to hollow porous structures, so that the insect source biological feed is very suitable for the propagation of probiotics and has higher space density. Therefore, the insect protein source biological feed can also decompose residual bait and excrement in a farm and degrade harmful substances such as ammonia nitrogen, nitrite and hydrogen sulfide, has good control effects on purifying the culture environment and inhibiting harmful bacteria, and can also greatly improve the conversion rate of the feed and reduce the cost of the feed. The tenebrio molitor dung sand and the barley pest dung sand can make up respective defects, promote respective advantages, well improve meat taste and flavor of economically cultured animals, have different amino acid ratios and fatty acid structures, and can better meet the requirements of the animals on specific amino acid through proportioning.

The short-chain fatty acid can increase the amount of intramuscular fat and intramyofiber of the meat, and can increase the meat-feeling elasticity and the fragrant and sweet flavor of the meat; the sodium glutamate can improve the delicate flavor and sweet taste of meat; the pine needle powder contains pine ester component capable of improving meat quality and flavor. The insect powder contains a large amount of chitin and shrimp shell powder, and the brittleness and toughness of the fish skin can be greatly improved.

The insect protein source biological feed prepared by the preparation method of the insect protein source biological feed can be preserved for more than one year, while the insect protein source biological feed in the prior art can be preserved for only 5 to 7 days. Can improve meat quality and flavor of animal, increase fiber and fat content of animal muscle, and increase lean meat percentage and snowflake meat ratio and flavor. Can prevent various diseases, improve intestinal function, improve feed utilization rate, improve production performance, improve meat quality and flavor, and can be used as selenium source additive for cultivating selenium-rich meat.

The preparation method of the insect protein source biological feed adopts the strain stock solution which can be fermented in advance and can also be obtained by other methods. When the fermentation is carried out in the step S4, the success rate of the fermentation can be improved, and the risk of infectious microbe infection is reduced, because the insect protein in the strain stock solution belongs to high-protein animal raw materials, the infectious microbes are easier to breed. Therefore, the fermented strain stock solution is directly used for secondary fermentation in the step S4 and other raw materials, so that the produced insect protein source biological feed is safer, the storage time is longer, and the quality is more stable.

Detailed Description

The following examples are given to further illustrate the embodiments of the present invention. The following examples are intended to illustrate the invention but are not intended to limit the scope of the invention.

In the description of the present invention, it should be noted that, unless otherwise explicitly specified or limited, the terms "mounted," "connected," and "connected" are to be construed broadly, e.g., as meaning either a fixed connection, a removable connection, or an integral connection; can be mechanically or electrically connected; they may be connected directly or indirectly through intervening media, or they may be interconnected between two elements. The specific meanings of the above terms in the present invention can be understood in specific cases to those skilled in the art.

A preparation method of insect protein source biological feed comprises the following steps:

s1, selecting the following first raw materials in percentage by weight: 10-15 parts of strain stock solution, 50-60 parts of brown sugar, 20-30 parts of selenium-enriched yeast, 10-15 parts of short-chain fatty acid and 5-10 parts of sodium glutamate;

s2, adding the raw materials into water which is 5-15 times the weight ratio of the raw materials to form a mixed solution, and standing the mixed solution in an environment with the temperature of 28-30 ℃ and the air humidity of 65-75%;

s3, selecting the following second raw materials in percentage by weight: 20-30 parts of enzyme residues, 20-30 parts of tenebrio molitor dung sand, 20-30 parts of barley pest dung sand, 5-10 parts of pine needle powder and 10-15 parts of corn powder, and mixing the second raw materials to form a mixture;

s4, uniformly mixing the static mixed liquid and the mixture according to the weight ratio of 1-3, and fermenting, wherein the fermentation process parameters are as follows: the temperature is 23-35 ℃, the air humidity is 65-75%, and the fermentation time is 3-5 days.

Specifically, S1, the following first raw materials are selected according to the weight ratio: 13 parts of strain stock solution, 55 parts of brown sugar, 25 parts of selenium-enriched yeast, 13 parts of short-chain fatty acid and 8 parts of sodium glutamate;

s2, adding the raw materials into water which is 10 times of the raw materials by weight to form a mixed solution, and standing the mixed solution in an environment with the temperature of 29 ℃ and the air humidity of 70%;

s3, selecting the following second raw materials in percentage by weight: 25 parts of enzyme residues, 25 parts of yellow mealworm excrement sand, 25 parts of barley worm excrement sand, 8 parts of pine needle powder and 13 parts of corn powder, and mixing the second raw materials to form a mixture;

s4, uniformly mixing the static mixed liquor and the mixture according to the weight ratio of 2, and then fermenting, wherein the fermentation process parameters are as follows: the temperature is 29 ℃, the air humidity is 70%, and the fermentation time is 4 days.

According to the preparation method of the insect protein source biological feed, the ferment residues can be reasonably utilized fully and reasonably, and meanwhile, the manufacturing cost of the insect protein source biological feed can be reduced. The ferment residues are rich in micromolecular peptides, antibacterial peptides, chitin, various trace elements, beneficial bacteria, enzyme preparations, animal and plant growth factors, vitamins, amino acids and the like which are rich in ferment, so that the prepared insect protein source biological feed is balanced and comprehensive in nutrition. A large amount of active insect antibacterial small peptides and probiotic bacteria are reserved in the enzyme residues, and the derivatives of the enzyme residues also contain a large amount of immune factors, so that the immunity of the organism can be greatly improved, the morbidity of cultured animals can be well prevented in the culture of the animals, and the survival rate of the animals is greatly improved.

The selenium-enriched yeast is rich in organic selenium, so that the organic selenium of animals can be improved, and the production of green pollution-free products is realized by using the organic selenium in the feed instead of antibiotics. And secondly, the short-chain fatty acid, pine needle powder and the like capable of improving fiber and fat between fleshy muscles are provided by the selenium-enriched yeast rich in organic selenium, so that a safe selenium source product without toxic and side effects is provided for enterprises breeding high-end selenium-enriched agricultural products, the healthy growth of animals is promoted, and a reliable choice is provided for the enterprises to produce high-quality selenium-enriched agricultural products integrating health and delicious taste.

The insect source biological feed of the embodiment can greatly improve the concentration and the activity of the probiotic groups by adding the yellow mealworm excrement sand and the barley pest excrement sand, and the yellow mealworm excrement sand and the barley pest excrement sand are not only high in protein content but also belong to hollow porous structures, so that the insect source biological feed is very suitable for the propagation of probiotics and is higher in space density. Therefore, the insect protein source biological feed can also decompose residual bait and excrement in a farm and degrade harmful substances such as ammonia nitrogen, nitrite and hydrogen sulfide, has good control effects on purifying the culture environment and inhibiting harmful bacteria, and can also greatly improve the conversion rate of the feed and reduce the cost of the feed. The tenebrio molitor dung sand and the barley pest dung sand can make up respective defects, promote respective advantages, well improve meat taste and flavor of economically cultured animals, have different amino acid ratios and fatty acid structures, and can better meet the requirements of the animals on specific amino acid through proportioning.

The short-chain fatty acid can increase the amount of intramuscular fat and intramyofiber of the meat, and can increase the meat-feeling elasticity and the fragrant and sweet flavor of the meat; the sodium glutamate can improve the delicate flavor and sweet taste of meat; the pine needle powder contains pine ester component capable of improving meat quality and flavor. The insect powder contains a large amount of chitin and shrimp shell powder, and the brittleness and toughness of the fish skin can be greatly improved.

The insect protein source biological feed prepared by the preparation method of the insect protein source biological feed can be preserved for more than one year, while the insect protein source biological feed in the prior art can be preserved for only 5-7 days. Can improve meat quality and flavor of animal, increase fiber and fat content of animal muscle, and increase lean meat percentage and snowflake meat ratio and flavor. Can prevent various diseases, improve intestinal function, improve feed utilization rate, improve production performance, improve meat quality and flavor, and can be used as selenium source additive for cultivating selenium-rich meat.

In the preparation method of the insect protein source biological feed, the adopted strain stock solution can be fermented in advance, and can also be obtained by other methods. When the fermentation is carried out in the step S4, the success rate of the fermentation can be improved, and the risk of infectious microbe infection is reduced, because the insect protein in the strain stock solution belongs to high-protein animal raw materials, the infectious microbes are easier to breed. Therefore, the fermented strain stock solution is directly used for secondary fermentation in the step S4 and other raw materials, so that the produced insect protein source biological feed is safer, the storage time is longer, and the quality is more stable.

Further, in one embodiment, in the step S1, the content of organic selenium in the selenium-enriched yeast is 2000-2400 mg/kg. Specifically, in step S1, the selenium-enriched yeast contains 2200mg/kg of organic selenium.

Further, in one embodiment, in step S1, the short chain fatty acid and the sodium glutamate are food grade, and the food grade is edible grade.

Further, in an embodiment, in step S1, the strain in the strain stock solution is yeast or lactic acid bacteria, which can well inhibit the growth and reproduction of other harmful bacteria.

Further, in one embodiment, in step S2, each raw material is added to 10 times by weight of water to form a mixed solution.

Further, in one embodiment, in step S2, the mixed solution is allowed to stand for 1 hour in an environment with a temperature of 28 to 30 ℃ and an air humidity of 65 to 75%. Specifically, in step S2, the mixed solution is allowed to stand for 1 hour in an environment at a temperature of 29 ℃ and an air humidity of 70%.

Further, in an embodiment, in step S3, the method for manufacturing the ferment slag includes the following steps:

grinding one or more of hermetia illucens, yellow mealworms, barley worms and earthworms into worm pulp, mixing the worm pulp, the biological enzyme and the fermentation strain for fermentation, and obtaining worm pulp enzyme and worm pulp enzyme residues after fermentation. The contents of the protein in the pulp ferment residues of yellow mealworms, barley worms, black soldier flies and the like are high, and the contents of substances such as glutamic acid which influences the meat quality and the delicate flavor are more abundant.

Further, in an embodiment, in step S4, the mixture after standing and the mixture are mixed uniformly according to the weight ratio of 1, and then the mixture is placed into a fermentation barrel for fermentation, which is convenient for fermentation.

Further, in an embodiment, in step S4, when the sweet smell and white hypha are generated by fermentation, it indicates that the fermented hypha is a finished feed product, and then the finished feed product is sequentially detected and packaged. The fermentation condition can be observed conveniently and timely, and abnormal conditions can be treated conveniently and timely.

Further, in an embodiment, in step S4, during the sub-packaging, the finished feed is packaged by using a breathing bag with a breathing valve, and a fiber bag is sleeved outside the breathing bag for packaging, transporting, storing and the like.

The above description is only a preferred embodiment of the present invention, and it should be noted that, for those skilled in the art, several modifications and variations can be made without departing from the technical principle of the present invention, and these modifications and variations should also be regarded as the protection scope of the present invention.