阅读说明：本技术 工程化嘌呤核苷磷酸化酶变体酶 (Engineered purine nucleoside phosphorylase variant enzymes ) 是由 斯科特·J·诺维克 尼基·德拉斯 韦丝娜·米切尔 段达 约瓦娜·纳佐尔 奥斯卡·阿尔维左 于 2019-07-02 设计创作，主要内容包括：本发明提供了工程化嘌呤核苷磷酸化酶(PNP)酶、具有PNP活性的多肽,和编码这些酶的多核苷酸,以及包含这些多核苷酸和多肽的载体和宿主细胞。还提供了用于产生PNP酶的方法。本发明还提供了包含PNP酶的组合物,以及使用工程化PNP酶的方法。本发明尤其可用于药物化合物的产生。(The invention provides engineered Purine Nucleoside Phosphorylase (PNP) enzymes, polypeptides having PNP activity, and polynucleotides encoding these enzymes, as well as vectors and host cells comprising these polynucleotides and polypeptides. Methods for producing PNP enzymes are also provided. The invention also provides compositions comprising PNP enzymes, and methods of using engineered PNP enzymes. The invention is particularly useful for the production of pharmaceutical compounds.)