阅读说明：本技术 促进钙吸收的发酵乳制品及其制备方法 (Fermented dairy product for promoting calcium absorption and preparation method thereof ) 是由 王世杰 冯丽莉 刘尧尧 袁庆彬 张栋 李艳 康志远 薛玉玲 韩美娜 荀一萍 封肖 于 2020-12-25 设计创作，主要内容包括：本发明属于乳制品加工领域,公开了一种促进钙吸收的发酵乳制品及其制备方法。所述发酵乳制品是通过将白砂糖加入到生牛乳中溶解,加热、均质、灭菌,加入复合益生菌,发酵后制得。本发明通过加入嗜热链球菌JMCC0030能够提高牛奶中钙元素的利用率,建立肠道菌群,提高人体对钙元素的吸收效果。本发明适用于一种发酵乳制品的制备,尤其适用于一种促进钙吸收的发酵乳制品的制备,所得促进钙吸收的发酵乳制品适合各类人群食用。(The invention belongs to the field of dairy product processing, and discloses a fermented dairy product for promoting calcium absorption and a preparation method thereof. The fermented dairy product is prepared by adding white granulated sugar into raw milk, dissolving, heating, homogenizing, sterilizing, adding composite probiotics, and fermenting. According to the invention, the streptococcus thermophilus JMCC0030 is added, so that the utilization rate of calcium in milk can be improved, intestinal flora is established, and the absorption effect of a human body on the calcium can be improved. The invention is suitable for preparing the fermented milk product, in particular to the fermented milk product for promoting the calcium absorption, and the fermented milk product for promoting the calcium absorption is suitable for various people.)

1. A fermented dairy product for promoting calcium absorption characterized by: the raw materials for preparing the active ingredients of the medicine comprise the following components in parts by weight: 900-950 parts of raw milk, 0-100 parts of white granulated sugar and composite probiotics.

2. A fermented dairy product promoting calcium absorption according to claim 1, characterized in that: the composite probiotics consist of lactobacillus bulgaricus JMCC0018 and streptococcus thermophilus JMCC 0030; the colony number of the lactobacillus bulgaricus JMCC0018 in raw cow milk is 1 multiplied by 10⁶~1×10⁸cfu/ml; the colony number of streptococcus thermophilus JMCC0030 in raw cow milk is 1 × 10⁶~1×10⁸cfu/ml。

3. A method of producing a fermented dairy product promoting calcium absorption according to claim 1 or 2, characterized in that: the method comprises the steps of adding white granulated sugar into raw milk, dissolving, heating, homogenizing, sterilizing, adding composite probiotics, and fermenting to obtain the fermented milk product for promoting calcium absorption.

4. The production method according to claim 3, characterized in that: the heating temperature is 58-70 ℃.

5. The production method according to claim 3 or 4, characterized in that: the homogenizing pressure is 16-20 MPa.

6. The production method according to claim 3 or 4, characterized in that: the temperature of sterilization is 134-138 ℃, and the time is 4-15 s.

7. The production method according to claim 3 or 4, characterized in that: the fermentation temperature is 38-42 ℃, and the fermentation time is 12-24 h; at the end of the fermentation, the acidity was 75 ° T.

Technical Field

The invention belongs to the field of dairy product processing, and relates to a fermented dairy product, in particular to a fermented dairy product for promoting calcium absorption.

Background

Calcium, an element essential to living beings. Ca is useful for human body in muscle, nerve, body fluid and bone²⁺A bound protein. Calcium is the main inorganic component of human bone and teeth, and is also an essential element for neurotransmission, muscle contraction, blood coagulation, hormone release, milk secretion and the like. Calcium accounts for about 1.4% of the total weight of the human body, participates in metabolism, and must be supplemented every day; the deficiency or excess of calcium in human body can affect growth and health.

At present, the main calcium supplement products in the market are mainly pure milk and health care products. Although the pure milk has high calcium content, the calcium element in the pure milk is rarely absorbed by the human body; the health care product for supplementing calcium is prepared by multiple medicines, and although calcium element in the health care product is easy to absorb, the health of human body is affected by side effects after long-term large-scale administration, so that potential safety hazards are caused.

Therefore, there is a need to develop a milk product which can make human body increase the utilization rate of calcium element in milk to supplement the daily calcium required by human body.

Disclosure of Invention

In order to overcome the defects in the prior art, the invention aims to provide a fermented milk product for promoting calcium absorption and a preparation method thereof, so as to achieve the purposes of improving the utilization rate of calcium in milk and improving the absorption rate of calcium.

In order to achieve the purpose, the technical scheme adopted by the invention is as follows:

a fermented milk product for promoting calcium absorption comprises the following raw materials of effective components in parts by weight: 900-950 parts of raw milk, 0-100 parts of white granulated sugar and composite probiotics;

as a limitation of the invention, the complex probiotics are composed of lactobacillus bulgaricus JMCC0018 and streptococcus thermophilus JMCC 0030; the colony number of the lactobacillus bulgaricus JMCC0018 in raw cow milk is 1 multiplied by 10⁶～1×10⁸cfu/ml; the colony number of the streptococcus thermophilus JMCC0030 in raw cow milk is 1 multiplied by 10⁶～1×10⁸cfu/ml；

The lactobacillus bulgaricus JMCC0018 is preserved in the China general microbiological culture Collection center of the culture Collection of microorganisms with the preservation number: CGMCC No. 14425; the streptococcus thermophilus JMCC0030 related by the invention is preserved in the China general microbiological culture Collection center of the China Committee for culture Collection of microorganisms with the preservation number: CGMCC NO.19075, which was first published in a prior application having application number 202011344643X;

the invention also provides a preparation method of the fermented dairy product for promoting calcium absorption, which comprises the steps of adding white granulated sugar into raw milk, dissolving, heating, homogenizing, sterilizing, adding composite probiotics, and fermenting to obtain the fermented dairy product for promoting calcium absorption;

as a third limitation of the invention, the heating temperature is 58-70 ℃;

as a fourth limitation of the present invention, the pressure of the homogenization is 16 to 20 MPa;

as a fifth limitation of the invention, the temperature of sterilization is 134-138 ℃ and the time is 4-15 s;

as a sixth limitation of the invention, the fermentation temperature is 38-42 ℃, the fermentation time is 12-24 h, and the acidity is 75 ° T;

due to the adoption of the technical scheme, compared with the prior art, the invention has the following beneficial effects:

(1) according to the fermented milk product for promoting calcium absorption, the streptococcus thermophilus JMCC0030 and the lactobacillus bulgaricus JMCC0018 are compounded, so that the apparent absorption rate of calcium can be improved, the blood calcium concentration is increased, and calcium absorption is facilitated;

(2) by adding streptococcus thermophilus JMCC0030 and lactobacillus bulgaricus JMCC0018 into the fermented dairy product for promoting calcium absorption, the fermented dairy product has the advantages of excellent tissue state, soft mouthfeel, delicious flavor and excellent sensory characteristics of the fermented dairy product;

(3) the preparation method of the fermented dairy product for promoting calcium absorption provided by the invention has the advantages of simple process technical steps and easily controlled process.

In conclusion, the fermented dairy product for promoting calcium absorption provided by the invention can improve the utilization rate of calcium element in milk by adding streptococcus thermophilus JMCC0030, establish intestinal flora and improve the absorption effect of human body on calcium element.

The invention is suitable for the preparation of a fermented dairy product, in particular for the preparation of a fermented dairy product for promoting calcium absorption; the obtained fermented milk product for promoting calcium absorption is suitable for all people.

Drawings

The invention is described in further detail below with reference to the figures and the embodiments.

FIG. 1 is a bar graph of the results of calcium apparent absorption in rats according to example 7 of the present invention;

FIG. 2 is a bar graph of the results of the calcium apparent absorption rate of rats in example 8 of the present invention.

Detailed Description

Preferred embodiments of the present invention will be described below with reference to the accompanying drawings. It should be understood that the description of the preferred embodiment is only for purposes of illustration and understanding, and is not intended to limit the invention.

Example 1 preparation of a fermented milk product A1 for promoting calcium absorption

This example 1 provides a method for preparing a fermented dairy product a1 for promoting calcium absorption, which comprises weighing 30kg of white sugar, adding to 900kg of raw milk heated to 58 ℃, stirring and dissolving, adjusting pressure to 20MPa, homogenizing, keeping the same pressure, continuing heating to 136 ℃, sterilizing for 4s, cooling to 42 ℃, adding 1 × 10⁶cfu/ml Lactobacillus bulgaricus JMCC0018 and 1X 10⁶And (3) fermenting cfu/ml streptococcus thermophilus JMCC0030 for 14h, monitoring the fermentation acidity to 75 DEG T, and stirring to break emulsion to obtain the fermented dairy product A1 for promoting calcium absorption.

Examples 2 to 6 methods for producing fermented milk products A2 to A6 that promote calcium absorption

Examples 2 to 6 respectively provide methods for preparing fermented dairy products a2 to a6 for promoting calcium absorption, which are basically the same as those in example 1, except that the raw material usage amount and some process parameters are different, and the specific process parameters are shown in table 1.

Table 1: process parameter table of fermented milk products A2-A6 for promoting calcium absorption

The other steps and parameters were the same as in example 1.

Example 7 Effect of Streptococcus thermophilus JMCC0030 on calcium uptake in rats

The effect of Streptococcus thermophilus JMCC0030 on the absorption rate of calcium in rats was studied in this example and specifically tested as follows.

The test substance: freeze drying Streptococcus thermophilus JMCC0030 to obtain powder;

comparison of test substances: freeze-drying streptococcus thermophilus JMCC0016 to prepare powder;

pretreatment: respectively and accurately weighing 2500mg of a test object, metering the volume to 100ml by using deionized water, uniformly mixing, and fresh preparing every day to obtain corresponding bacterial powder suspension;

test animals: 1 week old clean male SD rat 9;

test feed: the calcium-containing synthetic feed is administered, and the calcium content of the feed is 1000 mg/kg.

The test method comprises the following steps: a total of 9 1-week-old clean male SD rats are selected and fed with the synthetic calcium-containing feed, and the synthetic calcium-containing feed is randomly divided into three groups of 3 rats each, wherein the three groups are respectively as follows: blank control group, JMCC0030 group, JMCC0016 group. Wherein, the blank control group is perfused with deionized water according to 0.3mL/100gBW daily, the JMCC0030 group is perfused with fresh JMCC0030 powder suspension prepared according to 0.3mL/100gBW daily, and the JMCC0016 group is perfused with fresh JMCC0016 powder suspension prepared according to 0.3mL/100gBW daily. The stomach is drenched continuously for 3 weeks every day, fresh excrement of rats is collected every week, freeze-dried and weighed, and the amount of the excrement calcium is measured. After the last gastric lavage, all rats are fasted for 12 hours without water supply, then blood is taken from eyeballs, and serum is separated.

The apparent absorption rate of calcium is calculated as follows:

apparent absorption rate of calcium%

The body weight results for each group of rats are shown in table 2, the calcium apparent absorption results are shown in fig. 1, and the blood calcium level results are shown in table 3.

Table 2 rat body weight results table

Group of	Initial body weight (g)	Body weight (g) at the end of the test
			Blank control group	25	107
JMCC0016 group	25	108
			JMCC0030 group	25	109

The results in table 2 show that there was no significant difference in body weight between the rats in each group, indicating that gavage with streptococcus thermophilus JMCC0030 and streptococcus thermophilus JMCC0016 at 3 weeks of age had no significant effect on the body weight of the rats in each group.

The results of the calcium apparent absorption rate measurements are shown in fig. 1, and the results show that the rats in the JMCC0030 group have higher calcium apparent absorption rate values than the other two groups, and have significant differences. The apparent calcium absorption rate of the rats in the JMCC0016 group is slightly higher than that of the rats in the blank control group, and the results are not obviously different. The test results show that the gavage streptococcus thermophilus JMCC0030 can improve the calcium apparent absorption rate of rats to a certain extent, and the improvement effect is more remarkable than that of JMCC 0016.

Table 3: blood calcium level results

The results show that the blood calcium concentration of rats in each group has no significant difference, which indicates that the gavage streptococcus thermophilus JMCC0030 and the streptococcus thermophilus JMCC0016 have no significant influence on the serum calcium concentration of the rats, and indicates that the JMCC0030 can not directly influence the serum calcium concentration.

Example 8 Effect of fermented milk products promoting calcium absorption on calcium absorption in rats

The effect of the fermented milk product for promoting calcium absorption on the calcium absorption rate of rats was studied, and the specific test is as follows.

The test substance: randomly drawing two fermented milk products for promoting calcium absorption provided in examples 1-6 and two fermented milk products provided in comparative examples;

comparative example fermented milk product B was prepared by the same preparation method as in example 1, except that 1X 10 S.thermophilus was used⁶cfu/ml Streptococcus thermophilus JMCC0016, other steps and process parameters are the same as example 1;

test animals: 1 week old cleaning grade male SD rat 12 in total;

test feed: the calcium-containing synthetic feed is administered, and the actual measurement value of the calcium-containing amount of the feed is 1000 mg/kg.

The test method comprises the following steps: 12 male SD rats of 1 week old clean grade are selected, and are fed with the synthetic calcium-containing feed, and the synthetic calcium-containing feed is randomly divided into four groups, wherein each group comprises 3 animals: blank control group, test group I, test group II and comparison group. Wherein, the blank control group is subjected to gastric lavage and deionized water filling according to 0.3mL/100gBW per day, the test group I is subjected to gastric lavage and deionized water filling according to 0.3mL/100gBW per day and fermented milk product A1 provided in the example 1, the test group II is subjected to gastric lavage and fermented milk product A2 provided in the example 2 according to 0.3mL/100gBW per day, and the comparison group is subjected to gastric lavage and fermented milk product B provided in the comparative example 1 according to 0.3mL/100 gBW. The stomach is drenched continuously for 3 weeks every day, fresh excrement of rats is collected every week, freeze-dried and weighed, and the amount of the excrement calcium is measured. After the last gastric lavage, all rats are fasted for 12 hours without water supply, then blood is taken from eyeballs, and serum is separated.

The apparent absorption rate of calcium is calculated as follows:

apparent absorption rate of calcium%

The body weight results for each group of rats are shown in table 4, the calcium apparent absorption results are shown in fig. 2, and the blood calcium level results are shown in table 5.

Table 4 rat body weight results

Group of	Initial body weight (g)	Body weight (g) at the end of the test
			Blank control group	21	104
Test group I	24	101
			Test group II	23	105
Comparison group	21	102

The results in table 4 show that there is no significant difference in body weight between rats in each group, indicating that gavage of the fermented dairy products of the examples and comparative examples, which are 3 weeks in duration, has no significant effect on the body weight of rats in each group.

The calcium apparent absorption rate detection results are shown in fig. 2, the calcium apparent absorption rates of the test group I and the test group II are obviously higher than those of the blank control group and the comparison group, and no obvious difference exists between the test group I and the test group II, so that the intake of the fermented dairy products provided by the test group I and the test group II is beneficial to improving the calcium apparent absorption rate in the rat body, and the effect is better than that of the comparative example.

Table 5: blood calcium level results table

Group of	Serum calcium concentration (mmol/L)
		Blank control group	2.31
Test group I	2.39
		Test group II	2.41
Comparison group	2.34

The result shows that the blood calcium concentration of rats in each group has no significant difference, but the blood calcium concentration values of the rats in the test group I, the test group II and the comparison group are higher than those of the blank control group, and the blood calcium concentration values of the rats in the test group I and the test group II are higher than those of the comparison group, so that the result shows that the fermented dairy product for promoting calcium absorption can improve the serum calcium concentration of the rats to a certain extent.

As can be seen from examples 7 and 8, the fermented milk products for promoting calcium absorption provided in examples 1 to 6 can improve the apparent absorption rate of calcium in rats, increase the serum calcium concentration in rats to a certain extent, and have the ability to promote calcium absorption.

Example 10 organoleptic evaluation of fermented milk products promoting calcium absorption

The fermented milk products A1-A6 capable of promoting calcium absorption and the fermented milk product B in example 1-6 were subjected to sensory evaluation, and the evaluation criteria are shown in Table 6, wherein the score of 1 indicates that the sensory description is contained and is very obvious, the score of 0.5 indicates that the sensory description is contained but is not obvious, and the score of 0 indicates that the sensory description is absent. The evaluation randomly calls 15 consumers in the consumer population, and the evaluation is taken as a sensory evaluator, and the specific evaluation result is shown in table 7.

Table 6: sensory evaluation scoring standard table

Table 7: sensory evaluation result table

Group of	Tissue state	Taste of the product	Flavor (I) and flavor (II)
				Fermented milk product A1	14	15	15
Fermented milk product A2	15	15	15
				Fermented milk product A3	13	14	14
Fermented milk product A4	14	14	13
				Fermented milk product A5	13	13	14
Fermented milk product A6	13	14	14
				Fermented milk product B	11	13	13

The results show that the tissue state score of the fermented dairy product B provided by the comparative example is obviously lower than that of the fermented dairy products A1-A6 provided by the examples 1-6, and the fermentation characteristic of the combination of the streptococcus thermophilus JMCC0030 and the Lactobacillus bulgaricus JMCC0018 is better than that of the combination of the streptococcus thermophilus JMC0016 and the Lactobacillus bulgaricus JMCC 0018.

Although the present invention has been described in detail with reference to the above embodiments, it will be apparent to those skilled in the art that modifications may be made to the embodiments described above, or equivalents may be substituted for elements thereof. Any modification, equivalent replacement, or improvement made within the spirit and principle of the present invention should be included in the protection scope of the present invention.