阅读说明：本技术 一种利用磁珠法提取血液基因组dna的试剂盒及其应用 (Kit for extracting blood genome DNA by using paramagnetic particle method and application thereof ) 是由 张立岩 于 2020-12-22 设计创作，主要内容包括：本发明公开了一种利用磁珠法提取血液基因组DNA的试剂盒及其应用,属于分子生物学领域。本发明的目的是为了提供一种安全快速从血液中提取基因组DNA的试剂盒,该试剂盒包括结合液、红细胞裂解液、磁珠、磁珠漂洗液和DNA洗脱液。本发明试剂盒不包含有机溶剂,对全血细胞进行裂解后,通过磁珠吸附DNA分子,再通过漂洗去除杂质,最后对磁珠上的DNA分子进行洗脱,获得高质量的基因组DNA。该试剂盒具有操作简单快速、提取效率高、成本低廉的优点,提取的血液基因组DNA纯度高,能够满足后续如PCR扩增、基因测序等科研或临床诊断分析。(The invention discloses a kit for extracting blood genome DNA by a paramagnetic particle method and application thereof, belonging to the field of molecular biology. The invention aims to provide a kit for safely and quickly extracting genomic DNA from blood, which comprises a binding solution, a red blood cell lysate, magnetic beads, a magnetic bead rinsing solution and DNA eluent. The kit does not contain an organic solvent, after cracking the whole blood cells, the DNA molecules are adsorbed by the magnetic beads, impurities are removed by rinsing, and finally the DNA molecules on the magnetic beads are eluted to obtain high-quality genome DNA. The kit has the advantages of simple and rapid operation, high extraction efficiency and low cost, and the extracted blood genome DNA has high purity, and can meet the requirements of follow-up scientific research or clinical diagnosis and analysis such as PCR amplification, gene sequencing and the like.)