阅读说明：本技术 一种褐藻寡糖提取方法 (Brown algae oligosaccharide extraction method ) 是由 刘伟 于 2019-09-16 设计创作，主要内容包括：本发明涉及肥料领域,具体为褐藻提取物用于制备农业肥料的用途。目的将褐藻提取物用于农业行业的肥料中,改变现有化学药物对环境的污染,以及提高植物抗病能力。将褐藻提取物与肥料进行混合用于植物施肥；所述褐藻提取物制备方法为：将深海褐藻12.65%、纯水80%、混合酶7%、裂解酶0.1%、CZK30复合酶0.15%、山梨酸钾0.1%混合而成；步骤为：将12.65%的深海褐藻粉碎后与80%的纯水混合搅拌；并加热至40度均速搅拌均匀,进行多步复合中温弱酸生物酶解,在40度中温,加入0.1%的裂解酶开始酶解,酶解时间为2小时,再加入0.15%的CZK30复合酶进行酶解5小时,最后加入7%的混合酶进行酶解5小时；调和PH值；再加入0.1%的山梨酸钾搅拌均匀,并推送到离心机将深海褐藻分离出褐藻提出物原液。(The invention relates to the field of fertilizers, in particular to application of brown algae extract in preparation of agricultural fertilizers. The brown algae extract is used in fertilizer in agricultural industry, and can change the environmental pollution caused by chemical medicine and raise the disease resistance of plant. Mixing the brown algae extract with a fertilizer for plant fertilization; the preparation method of the brown algae extract comprises the following steps: mixing deep sea brown algae 12.65%, pure water 80%, mixed enzyme 7%, lyase 0.1%, CZK30 complex enzyme 0.15%, and potassium sorbate 0.1%; the method comprises the following steps: pulverizing deep-sea brown algae 12.65%, mixing with pure water 80%, and stirring; heating to 40 ℃, uniformly stirring at a uniform speed, performing multi-step composite medium-temperature weak acid biological enzymolysis, adding 0.1% of lyase at the medium temperature of 40 ℃ to start enzymolysis for 2 hours, adding 0.15% of CZK30 complex enzyme to perform enzymolysis for 5 hours, and finally adding 7% of mixed enzyme to perform enzymolysis for 5 hours; adjusting the pH value; adding 0.1% potassium sorbate, stirring, and delivering to a centrifuge to separate deep sea brown algae to obtain brown algae extract stock solution.)

1. A brown algae oligosaccharide extraction method is characterized by comprising 12.65% of dry brown algae, 80% of pure water, 7% of mixed enzyme, 0.1% of lyase, 0.15% of CZK30 complex enzyme and 0.1% of potassium sorbate;

the method comprises the following seven steps:

step one, breaking the wall of 12.65% dry brown algae to 200 meshes by high-power dust-free crushing, and the step comprises the following three procedures:

1) feeding dry brown algae to high-power dust-free crushing,

2) the processing from coarse crushing to fine crushing is carried out,

3) screening and detecting the powder after wall breaking, and entering the next step if the powder is qualified;

step two, pumping the dry brown algae powder obtained in the step 1 into a stirring tank;

step three, adding 80% of pure water and dry brown algae powder into a stirring tank, and mixing and stirring;

step four, heating the semi-finished products obtained in the step 1 to the step three to 40 ℃, and uniformly stirring at a uniform speed;

step five, carrying out multi-step composite moderate-temperature weak acid biological enzymolysis, wherein the step comprises the following three procedures:

1) adding 0.1% of lyase at the medium temperature of 40 ℃ to start enzymolysis, wherein the enzymolysis time is 2 hours,

2) then 0.15 percent of CZK30 complex enzyme is added for enzymolysis for 5 hours,

3) finally adding 7% of mixed enzyme for enzymolysis for 5 hours;

step six, detecting and adjusting the pH value;

and step seven, separation.

2. The method of claim 1, wherein the detection criteria in step 6 are: the pH was 7.

3. The method for extracting alginate oligosaccharides according to claim 1 or 2, wherein 0.1% potassium sorbate is added after the steps are completed, the mixture is stirred uniformly and is sent to a centrifuge to separate alginate oligosaccharide stock solution from dry brown algae.

Technical Field

The invention relates to the field of brown algae extracts, in particular to a brown algae oligosaccharide extraction method.

Background

The brown algae oligosaccharide is a latest generation functional oligosaccharide, is a brown algae oligosaccharide with the polymerization degree of less than 20 obtained by adopting an internationally advanced separation and degradation technology, and is a linear oligomer consisting of beta-D-mannuronic acid and alpha-L-guluronic acid.

The technical advantages are as follows: the brown algae oligosaccharide is derived from pure natural and healthy algae, has the excellent characteristics of the algae, extends a plurality of new biological functions, and is incomparable with other oligosaccharides.

The composite material can be applied to a plurality of industries such as medicine and health care product industry, feed industry, agricultural industry, chemical product industry and the like, and the composite material has the following properties in the medicine and health care product industry: can be used for the adjuvant treatment of hypertension, diabetes, osteoporosis, senile dementia, and constipation. The food product can be applied to the food industry: milk, cholesterol-lowering beverage, seaweed soda water (radiation-resistant and fatigue-relieving), cookies, etc. Can be used in the feed industry for: livestock and poultry feed additive and aquatic animal feed additive. Can be used as follows in the agricultural industry: foliage fertilizer, water flush fertilizer and seed coating agent. In skin care products, the following can be prepared: various lotions, creams, essences, etc. Can be used for manufacturing in the daily chemical product industry: hand sanitizer, shampoo, hair conditioner, laundry detergent, toothpaste, soap and the like.

The existing brown algae extraction technology has complex process, high cost and low extraction purity, and causes great waste.

Disclosure of Invention

The invention aims to solve the problems of complicated process, high cost, low extraction purity and great waste of the existing brown algae extraction technology.

In order to solve the technical problem, the invention provides a brown alga oligosaccharide extraction method, which comprises the steps of mixing and preparing dry brown alga 12.65%, pure water 80%, mixed enzyme 7%, lyase 0.1%, CZK30 complex enzyme 0.15%, and potassium sorbate 0.1%;

the method comprises the following seven steps:

step one, breaking the wall of 12.65% dry brown algae to 200 meshes by high-power dust-free crushing, and the step comprises the following three procedures:

1) feeding dry brown algae to high-power dust-free crushing,

2) the processing from coarse crushing to fine crushing is carried out,

3) screening and detecting the powder after wall breaking, and entering the next step if the powder is qualified;

step two, pumping the dry brown algae powder obtained in the step 1 into a stirring tank;

step three, adding 80% of pure water and dry brown algae powder into a stirring tank, and mixing and stirring;

step four, heating the semi-finished products obtained in the step 1 to the step three to 40 ℃, and uniformly stirring at a uniform speed;

step five, carrying out multi-step composite moderate-temperature weak acid biological enzymolysis, wherein the step comprises the following three procedures:

1) adding 0.1% of lyase at the medium temperature of 40 ℃ to start enzymolysis, wherein the enzymolysis time is 2 hours,

2) then 0.15 percent of CZK30 complex enzyme is added for enzymolysis for 5 hours,

3) finally adding 7% of mixed enzyme for enzymolysis for 5 hours;

step six, detecting and adjusting the pH value;

and step seven, separation.

Wherein the detection criteria in step 6 are: the pH was 7.

Further comprises the steps of adding 0.1 percent of potassium sorbate after the steps are finished, stirring evenly, and pushing the mixture to a centrifuge to separate out the alginate oligosaccharide stock solution from the dry brown algae.

Has the advantages that:

the invention has the following innovative advantages,

According to the brown alga oligosaccharide extraction method, through multi-step composite medium-temperature weak acid biological enzymolysis, lyase, CZK30 complex enzyme and mixed enzyme are adopted to connect enzymolysis for three times, so that the separated brown alga oligosaccharide stock solution is purer, the peptide and sugar activities are higher, and the enzymolysis is more thorough, so that bioactive substances and nutrient substances in brown alga are retained to the maximum extent, polysaccharide substances are degraded into tiny oligosaccharides, the compatibility is good, and the brown alga oligosaccharide can be easily absorbed no matter being applied to animals and plants.

Drawings

FIG. 1 is a flow chart of the present invention;

FIG. 2 is a diagram showing the components of COA detection according to the present invention.

Detailed Description

The invention is further illustrated by the following figures and examples.

The invention relates to a brown alga oligosaccharide extraction method, which ensures that the extracted brown alga oligosaccharide is purer, higher in peptide and sugar activities and more thorough in enzymolysis through multi-step composite moderate-temperature weak-acid biological enzymolysis, so that bioactive substances and nutrient substances in brown alga are retained to the maximum extent, polysaccharide substances are degraded into extremely fine oligosaccharides, the compatibility is excellent, and animals and plants are very easy to absorb.

As shown in figure 1, the brown algae oligosaccharide extraction method comprises the steps of mixing and preparing dry brown algae 12.65%, pure water 80%, mixed enzyme 7%, lyase 0.1%, CZK30 complex enzyme 0.15%, and potassium sorbate 0.1%;

the preparation process comprises the following steps: breaking the wall of 12.65% dry brown algae to 200 meshes by high-power dust-free crushing, and the method comprises the following three steps:

1) feeding dry brown algae to high-power dust-free crushing,

2) the processing from coarse crushing to fine crushing is carried out,

3) screening and detecting the powder after wall breaking, and entering the next step if the powder is qualified;

pumping the crushed dry brown algae powder into a stirring tank for stirring,

then 80% pure water and dry brown algae powder are added into the stirring tank to be mixed and stirred,

heating the semi-finished product obtained in the above steps to 40 ℃ after stirring, uniformly stirring at a uniform speed,

stirring and then carrying out multi-step composite moderate-temperature weak acid biological enzymolysis, wherein the steps comprise the following three procedures:

1) under the condition of keeping at the medium temperature of 40 ℃, 0.1 percent of lyase is added to start enzymolysis, the enzymolysis time is 2 hours,

2) then 0.15 percent of CZK30 complex enzyme is added for enzymolysis for 5 hours,

3) finally adding 7% of mixed enzyme for enzymolysis for 5 hours;

detecting after completion, performing enzymolysis again if the product is unqualified, entering the next step if the product is qualified, adjusting the pH value to 7,

and finally, separating, wherein the separation process comprises the following steps: and adding 0.1% of potassium sorbate into the semi-finished product obtained in the step again, uniformly stirring, and pushing the mixture to a centrifuge to separate out brown alginate oligosaccharide stock solution from dry brown algae.

As shown in FIG. 2, the brown algae oligosaccharide stock solution separated by the brown algae oligosaccharide extraction method of the invention,

the identification of Analysis (COA) detection Analysis comprises the following components:

indole Auxin/Indole Auxin 3ppm

Endogenous Cytokinins/cytokines 0.012ppm

Vitamin B1/vb 123 ppm

Vitamin B2/v B215 ppm

Vitamin B12/Vb120.02ppm

Vitamin C/Vc 900ppm

Organic matter/Organic matter 55 g/L

Oligosaccharide/Oligosaccharide 38 g/L

Alginic acid/Alginic acid 3g/L

Mannitol/Mannitol 23 g/L

boron/B3.2 g/L

calcium/Ca 4.1 g/L

The above-described preferred embodiments of the present invention are not intended to limit the present invention, and any modifications made within the spirit and principle of the present invention should be included within the scope of the present invention.