阅读说明：本技术 一种微生物法制备复合抑藻剂的方法 (Method for preparing composite algistat by microbiological method ) 是由 赵亚灿 于 2020-07-09 设计创作，主要内容包括：本发明涉及一种微生物法制备复合抑藻剂的方法,包括以下步骤：(1)将大麦秸秆、芦苇和艾草粉碎成60mm-100mm的颗粒,然后加水,温度30-40℃,浸泡3-5天；(2)然后加入黄连粉,浸泡1-2天；(3)过滤,得第一滤液和滤渣,所述的滤渣加水浸泡,然后添加纤维素酶,温度40-50℃,酶解1-2h；(4)过滤,得第二滤液和滤渣,将第二滤液在90-100℃处理8-10min,然后冷却,过滤,得第三滤液；(5)合并混匀第一滤液和第三滤液,过滤,得复合抑藻剂。本发明的抑藻剂相对于单独采用大麦秸秆作为抑藻剂,抑藻效果更好,对多种藻类都具有良好的抑制效果,而且保存期长,保存6个月,抑藻效果几乎没有变化。(The invention relates to a method for preparing a composite algicide by a microbiological method, which comprises the following steps: (1) pulverizing barley straw, reed and folium Artemisiae Argyi into 60-100 mm granules, adding water, soaking at 30-40 deg.C for 3-5 days; (2) then adding the coptis powder, and soaking for 1-2 days; (3) filtering to obtain a first filtrate and filter residue, soaking the filter residue in water, adding cellulase, and performing enzymolysis at 40-50 deg.C for 1-2 hr; (4) filtering to obtain a second filtrate and filter residue, treating the second filtrate at 90-100 deg.C for 8-10min, cooling, and filtering to obtain a third filtrate; (5) and combining and uniformly mixing the first filtrate and the third filtrate, and filtering to obtain the composite algistat. Compared with the method of singly adopting barley straws as the algae inhibitor, the algae inhibitor has better algae inhibiting effect, has good inhibiting effect on various algae, and has long storage period of 6 months, and almost no change in the algae inhibiting effect.)

1. A method for preparing a composite algistat by a microbiological method is characterized by comprising the following steps:

(1) Pulverizing barley straw, reed and folium Artemisiae Argyi into 60-100 mm granules, adding water, soaking at 30-40 deg.C for 3-5 days;

(2) then adding the coptis powder, and soaking for 1-2 days;

(3) filtering to obtain a first filtrate and filter residue, soaking the filter residue in water, adding cellulase, and performing enzymolysis at 40-50 deg.C for 1-2 hr;

(4) filtering to obtain a second filtrate and filter residue, treating the second filtrate at 90-100 deg.C for 8-10min, cooling, and filtering to obtain a third filtrate;

(5) and combining and uniformly mixing the first filtrate and the third filtrate, and filtering to obtain the composite algistat.

2. The method for preparing the compound algistat by the microbiological method according to claim 1, wherein the method comprises the following steps: the barley straw, the reed, the wormwood and the coptis chinensis powder are added according to the weight parts of 60-70 parts of barley straw, 30-40 parts of reed, 5-10 parts of wormwood and 1-2 parts of coptis chinensis powder.

3. The method for preparing the compound algistat by the microbiological method according to claim 1, wherein the method comprises the following steps:

the barley straws, the reeds and the wormwood are crushed into particles of 60mm-100mm, and the volume of water added is 1g, namely 20ml-30 ml.

4. The method for preparing the compound algistat by the microbiological method according to claim 1, wherein the method comprises the following steps:

And the filter residue is soaked in water, and the volume of the water added in the soaking process is 1g, 5ml to 10 ml.

5. The method for preparing the compound algistat by the microbiological method according to claim 1, wherein the method comprises the following steps: the addition amount of the cellulase is 0.2-0.5 percent of the total weight of the barley straws, the reeds, the wormwood and the coptis chinensis powder.

6. The method for preparing the compound algistat by the microbiological method according to claim 5, wherein the method comprises the following steps:

the enzyme activity of the cellulase is 10-15 ten thousand U/g.

7. The method for preparing the composite algistat by the microbiological method according to any one of claims 1 to 6, wherein: in the soaking process of the step (1), 0.1-0.2 percent of lactic acid bacteria and 0.05-0.1 percent of rhizopus are added.

8. The method for preparing a compound algistat by a microbiological method according to claim, wherein the method comprises the following steps: each gram of the lactobacillus contains 30 hundred million live bacteria.

Technical Field

The invention relates to a method for preparing a composite algicide by a microbiological method, and belongs to the field of ecological algae control.

Background

In recent years, harmful algal blooms frequently occur in lakes and reservoirs, and particularly in summer, ecological hazards are brought to influence drinking water safety. The outbreak of algae bloom and the eutrophication of water are inseparable, and the mass propagation of algae causes the drinking water to be smelly, changes the structure of lake communities and reduces the biodiversity. How to effectively control the algae in the eutrophic water body and prevent the outbreak of the water bloom becomes a research hotspot and a leading-edge problem in the current environmental protection field. The traditional algae removal technology can be roughly divided into three types, namely physical technology, chemical technology and biological technology. The chemical technology is more commonly used, the growth of algae can be temporarily alleviated by adding algicides such as copper sulphate, diluted acid, potassium permanganate and the like, but the cost is high, the algicides also have toxic action on other aquatic organisms, and the ecological balance is inevitably destroyed and secondary environmental pollution is caused. The physical technology has the disadvantages of long operation time, high difficulty, high cost and the like. For example, the manual catching method generally accepted by governments in various regions not only has low algae removal efficiency, but also can artificially change the structure of an aquatic ecosystem, and has high cost for safely disposing the algae residues on the market. The traditional biological control method mainly aims to prevent the occurrence of water bloom by artificially increasing certain organisms which can directly feed on the algae or indirectly inhibit the growth of the algae, but has the disadvantages of more complex specific application process, larger labor capacity, higher cost and longer treatment period compared with chemical and physical methods. In recent years, the discovery of the inhibiting effect of plants on the allelopathy of algae leads the allelopathy to be applied to the fields of eutrophic water body treatment and algae control, and related researches have been widely concerned at home and abroad. The allelochemicals are secondary metabolites generated in the plant growth process, have good specificity on allelochemicals of algae, and can be generally degraded under natural conditions. Therefore, the phytochemical sensing algae control method is a method with good ecological safety and good application prospect. The application example of controlling the puffiness by utilizing allelopathy is the most successful application example up to now by utilizing the barley inserted rod to be thrown into the water body to inhibit the algae. However, there are the following problems: (1) allelochemicals have obvious selectivity on the inhibition effect of algae and have obvious inhibition effect on specific algae, but the algae community in the natural water body is composed of a plurality of algae, so that the effect is not good; (2) the existing method for preparing the algae inhibiting agent by using the straw extract has the problem of short storage period, and the algae inhibiting effect is reduced by more than 50 percent due to the fact that the algae inhibiting agent is stored hermetically for 3-6 months generally, so that the algae inhibiting agent cannot be stored and used for a long time. (3) Some medicinal plants also find the algae inhibiting effect at present, have good effect, but have high cost and cannot be used in large scale.

Disclosure of Invention

The invention provides a method for preparing a composite algistat by a microbiological method, the prepared algistat takes barley straws as main raw materials, is prepared by a biological method, has the advantages of low cost, comprehensive algae inhibition, good effect and long storage life, and effectively solves the problems of the existing plant algistat.

In order to solve the technical problems, the technical scheme adopted by the invention is as follows:

a method for preparing a composite algistat by a microbiological method comprises the following steps:

(1) pulverizing barley straw, reed and folium Artemisiae Argyi into 60-100 mm granules, adding water, soaking at 30-40 deg.C for 3-5 days;

(2) then adding the coptis powder, and soaking for 1-2 days;

(3) filtering to obtain a first filtrate and filter residue, soaking the filter residue in water, adding cellulase, and performing enzymolysis at 40-50 deg.C for 1-2 hr;

(4) filtering to obtain a second filtrate and filter residue, treating the second filtrate at 90-100 deg.C for 8-10min, cooling, and filtering to obtain a third filtrate;

(5) and combining and uniformly mixing the first filtrate and the third filtrate, and filtering to obtain the composite algistat.

Preferably: the barley straw, the reed, the wormwood and the coptis chinensis powder are added according to the weight parts of 60-70 parts of barley straw, 30-40 parts of reed, 5-10 parts of wormwood and 1-2 parts of coptis chinensis powder.

Preferably: the barley straws, the reeds and the wormwood are crushed into particles of 60mm-100mm, and the volume of water added is 1g, namely 20ml-30 ml.

Preferably: and the filter residue is soaked in water, and the volume of the water added in the soaking process is 1g, 5ml to 10 ml.

Preferably: the addition amount of the cellulase is 0.2-0.5 percent of the total weight of the barley straws, the reeds, the wormwood and the coptis chinensis powder.

Preferably: the enzyme activity of the cellulase is 10-15 ten thousand U/g.

Preferably: in the soaking process of the step (1), 0.1-0.2 percent of lactic acid bacteria and 0.05-0.1 percent of rhizopus are added.

Preferably: each gram of the lactobacillus contains 30 hundred million live bacteria.

The invention has the beneficial effects that:

according to the invention, the barley straws are used as main auxiliary materials, the reed and the wormwood are compounded, the soaking fermentation is carried out, the coptis chinensis powder is added at the later stage, then the filtration is carried out, the filter residue is subjected to enzymolysis, the filtration is carried out, and the filtrate is combined, so that the algae inhibiting agent is obtained. The algae inhibitor of the invention adopts plant waste as main raw materials, has low cost and simple preparation method, and filter residue can be used as raw materials of bio-fertilizer, and is easier to decompose and utilize compared with straw used as raw materials of bio-fertilizer.

Detailed Description

The technical solutions of the present invention will be described clearly and completely with reference to the following embodiments of the present invention, and it should be understood that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.

Algae inhibition effect experiment:

algae species:

microcystis aeruginosa: accession number FACHB-315, medium: BG11, sourced from Wuhan aquatic organism research of Chinese academy of sciences;

chlorella: accession number FACHB-26, medium: BG11, sourced from Wuhan aquatic organism research of Chinese academy of sciences;

crescent moon algae: accession number FACHB-271, medium: BG11 from Wuhan aquatic organism research of Chinese academy of sciences;

the number of glomus sp: FACHB-2077, medium: BG11 from Wuhan aquatic organism research of Chinese academy of sciences;

the number of the Cyclotella is as follows: FACHB-1638, Medium: CSI, sourced from Wuhan aquatic organism research of Chinese academy of sciences;

scenedesmus obliquus is numbered: FACHB-12BG11, Medium: BG11 from Wuhan aquatic organism research of Chinese academy of sciences;

the inhibition ratio formula is as follows: IR ═ 1-N/N0 (X100%)

In the formula, IR-inhibition rate;

n-density of algae added with algistat (pieces/ml);

n0 — control group algal density (pieces/ml).

Algae inhibition effect experiment:

six kinds of algae were cultured to logarithmic phase according to the culture instructions, and then the logarithmic phase algae was added to a 250ml culture flask and diluted to 4.0X 10 with the medium⁶cells/ml, the volume of the solution is 100ml, then 1ml of the algistat prepared by the embodiment of the invention is added, meanwhile, the group without cross algistat is set as a control group, three groups are parallel, and the cells are cultured for 5 days for algae cell calculation. The culture conditions of each group are 4000lx according to the standard, the light-dark ratio of 12:12 and the temperature (25 +/-1) DEG C.

The barley straw of the invention adopts the No. 4 straw of resident barley. The barley straw, reed and wormwood powder are all dry products.