阅读说明：本技术 基于细胞工程透皮给营养的纤连蛋白抗衰再生修复方法 (Cell engineering transdermal nutrition-based fibronectin anti-aging regeneration repair method ) 是由 廖籽淳 于 2021-06-25 设计创作，主要内容包括：本发明提供了一种基于细胞工程透皮给营养的纤连蛋白抗衰再生修复方法,本发明的修复方法包括先清洗需要处理的区域,之后进行标注；对标注的范围注入麻剂,再次清洗后的标注范围消毒,然后将细胞纤连蛋白制剂导入标注范围。可见,本申请的方法操作简单,无疤痕,不易受细菌等感染。本发明的细胞纤连蛋白制剂的原料包括细胞纤连蛋白、甘氨酸、氯化钠、聚谷氨酸、β-葡聚糖和生理盐水,由于细胞纤连蛋白和聚谷氨酸、β-葡聚糖相结合,使本申请的制剂具有良好的抗衰再生效果,而本申请提供的细胞纤连蛋白制剂的制备方法对能够充分融合各组分功效的同时,充分保留各组分的有效成分。(The invention provides a cell engineering transdermal nutrition-based fibronectin anti-aging regeneration repair method, which comprises the steps of cleaning an area to be treated, and labeling; the labeled ranges were infused with anesthetic, the labeled ranges were again washed and sterilized, and then the cellular fibronectin preparation was introduced into the labeled ranges. Therefore, the method is simple to operate, has no scars and is not easy to be infected by bacteria and the like. The raw materials of the cellular fibronectin preparation comprise cellular fibronectin, glycine, sodium chloride, polyglutamic acid, beta-glucan and normal saline, and the cellular fibronectin, the polyglutamic acid and the beta-glucan are combined, so that the preparation has good anti-aging regeneration effect, and the preparation method of the cellular fibronectin preparation provided by the application can fully fuse the effects of the components and fully reserve the effective components of the components.)

1. A fibronectin anti-aging regeneration repair method based on cell engineering transdermal nutrition supply is characterized by comprising the following steps:

firstly, cleaning an area needing to be processed, and then labeling;

step two, injecting anesthetic into the range marked in the step one, and cleaning the marked range again after the anesthetic exerts the drug effect;

and step three, sterilizing the marked range cleaned again in the step two, introducing the cellular fibronectin preparation into the marked range, and then applying the cellular fibronectin preparation for 10-20min by using water at the temperature of 10-20 ℃.

2. A cellular fibronectin preparation, comprising: the cellular fibronectin preparation is used for the cellular engineering transdermal nutrition-based fibronectin anti-aging regeneration repair method of claim 1.

3. The cellular fibronectin preparation of claim 2, wherein: the raw materials for preparing the cellular fibronectin preparation include cellular fibronectin, glycine, sodium chloride, polyglutamic acid, β -glucan, and physiological saline.

4. The cellular fibronectin preparation of claim 3, wherein the raw materials for preparing the cellular fibronectin preparation comprise the following components in parts by weight: 0.4-0.6 part of cellular fibronectin, 2-4 parts of glycine, 1-2 parts of sodium chloride, 0.1-0.3 part of polyglutamic acid, 4-6 parts of beta-glucan and 40-100 parts of physiological saline.

5. The cellular fibronectin preparation of claim 4, wherein: the cellular fibronectin concentration was 1.2-1.4 mg/mL.

6. A method of preparing the cellular fibronectin preparation of any of claims 2-5, comprising the steps of:

step one, dividing normal saline into a first solution and a second solution according to the volume ratio of 4: 1; putting the first solution and the second solution into two reaction kettles, and adding cellular fibronectin into the first solution for later use;

step two, sequentially adding glycine, sodium chloride and polyglutamic acid into the first solution added with cellular fibronectin in the step one to prepare a mixture for later use;

and step three, adding the second solution and the beta-glucan into the mixture prepared in the step two at the same time, stirring while adding, and uniformly stirring to prepare the cellular fibronectin preparation.

7. The method according to claim 6, wherein the steps one to three are carried out at a temperature of 15 to 30 ℃.

8. The method according to claim 6, wherein the method for producing β -glucan comprises the steps of:

puffing grains to obtain puffed material, mixing puffed material with water, adjusting pH of the extractive solution, and performing solid-liquid separation; adding amylase into the separated liquid, and separating, purifying and concentrating after saccharification reaction to obtain the beta-glucan.

9. The method of claim 8, wherein the puffed material is mixed with water at a mass ratio of 1: 10-20, the pH is adjusted to 6-14, and the mixture is extracted at 30-100 ℃ for 0.5-10 hours.

10. Use of the cell-engineered, transdermally-administered, fibronectin anti-aging regenerative repair method of claim 1 for anti-aging.

Technical Field

The invention relates to the technical field of biology, in particular to a cell engineering transdermal nutrition-based fibronectin anti-aging regeneration repair method.

Background

Fibronectin, a high molecular weight glycoprotein in the extracellular matrix, is a major cell adhesion molecule that exerts structural and adhesive effects in the cellular fibrous matrix. Fibronectin can promote cell growth, improve cell adherence rate, enhance cell metabolism, shorten cell growth time, and improve cell fusion rate in monoclonal antibody preparation technology. Fibronectin exists mainly in three forms, namely plasma fibronectin produced by hepatocytes or endothelial cells, cellular fibronectin secreted by fibroblasts, early mesenchymal cells and fetal fibronectin in placenta, amniotic tissue.

At present, cellular fibronectin is not widely applied in the anti-aging regeneration and repair process, so that the provision of a cellular fibronectin anti-aging regeneration and repair method has important significance.

Disclosure of Invention

The invention aims to provide a cell engineering transdermal nutrition-based fibronectin anti-aging regeneration repair method, which can be widely applied to anti-aging regeneration repair of skin.

In order to achieve the above object, the embodiments of the present invention provide the following technical solutions:

according to a first aspect of embodiments of the present invention, there is provided a method for cytoengineered transdermal nutritional fibronectin anti-aging regeneration repair, comprising the steps of:

firstly, cleaning an area needing to be processed, and then labeling;

step two, injecting anesthetic into the range marked in the step one, and cleaning the marked range again after the anesthetic exerts the drug effect;

and step three, sterilizing the marked range cleaned again in the step two, introducing the cellular fibronectin preparation into the marked range, and then applying the cellular fibronectin preparation for 10-20min by using water at the temperature of 10-20 ℃.

According to a second aspect of embodiments of the present invention there is provided a cellular fibronectin preparation for use in the cell engineering based transdermal nutrient-delivered fibronectin anti-aging regenerative repair method.

Further, the raw materials for preparing the cellular fibronectin preparation include cellular fibronectin, glycine, sodium chloride, polyglutamic acid, β -glucan, and physiological saline.

Further, the raw materials for preparing the cellular fibronectin preparation comprise the following components in parts by weight: 0.4-0.6 part of cellular fibronectin, 2-4 parts of glycine, 1-2 parts of sodium chloride, 0.1-0.3 part of polyglutamic acid, 4-6 parts of beta-glucan and 40-100 parts of physiological saline.

Further, the cellular fibronectin concentration is 1.2-1.4 mg/mL.

According to a third aspect of embodiments of the present invention there is provided a method of preparing a cellular fibronectin preparation comprising the steps of:

step one, dividing normal saline into a first solution and a second solution according to the volume ratio of 4: 1; putting the first solution and the second solution into two reaction kettles, and adding cellular fibronectin into the first solution for later use;

step two, sequentially adding glycine, sodium chloride and polyglutamic acid into the first solution added with cellular fibronectin in the step one to prepare a mixture for later use;

and step three, adding the second solution and the beta-glucan into the mixture prepared in the step two at the same time, stirring while adding, and uniformly stirring to prepare the cellular fibronectin preparation.

Further, the first step, the second step and the third step are carried out at the temperature of 15-30 ℃.

Further, the preparation method of the beta-glucan comprises the following steps: puffing grains to obtain puffed material, mixing puffed material with water, adjusting pH of the extractive solution, and performing solid-liquid separation; adding amylase into the separated liquid, and separating, purifying and concentrating after saccharification reaction to obtain the beta-glucan. The puffing treatment is airflow puffing or extrusion puffing.

Further, the puffed material and water are fully mixed according to the mass ratio of 1: 10-20, the pH value is adjusted to 6-14, and the mixture is extracted for 0.5-10 hours at the temperature of 30-100 ℃.

According to a first aspect of embodiments of the present invention, there is provided a use of a cell-engineering transdermal nutrient-based fibronectin anti-aging regenerative repair method for anti-aging.

The embodiment of the invention has the following advantages: the embodiment of the invention provides a cell engineering transdermal nutrition-based fibronectin anti-aging regeneration repair method which is simple to operate, only needs to select a treated area, then cleans, injects anesthetic, sterilizes and introduces cellular fibronectin preparation, has no scar and is not easy to be infected by bacteria and the like. The raw materials of the cellular fibronectin preparation comprise cellular fibronectin, glycine, sodium chloride, polyglutamic acid, beta-glucan and normal saline, and the cellular fibronectin, the polyglutamic acid and the beta-glucan are combined, so that the preparation has good anti-aging regeneration effect, and the preparation method of the cellular fibronectin preparation provided by the application can fully fuse the effects of the components and fully reserve the effective components of the components.

Detailed Description

The technical solutions of the present invention will be described clearly and completely with reference to the following embodiments, and it should be understood that the described embodiments are some, but not all, embodiments of the present invention. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.

Example 1

The embodiment provides a cell engineering transdermal nutrition-based fibronectin anti-aging regeneration repair method, which comprises the following steps:

firstly, cleaning an area needing to be processed, and then labeling;

step two, injecting anesthetic into the range marked in the step one, and cleaning the marked range again after the anesthetic exerts the drug effect;

and step three, sterilizing the marked range after the second cleaning, introducing the cellular fibronectin preparation into the marked range, and applying the cellular fibronectin preparation for 10-20min by using water at the temperature of 10-20 ℃.

The repair method provided by the embodiment is simple to operate, only needs to select the treated area, then cleans, injects anesthetic, sterilizes and introduces the cellular fibronectin preparation, has no scar, and is free from infection of bacteria and the like.

Example 2

This example provides a cellular fibronectin preparation for use in the method of anti-aging regenerative repair of cellular fibronectin. The cellular fibronectin preparation is prepared from the following raw materials in parts by weight: 0.4 part of cellular fibronectin, 2 parts of glycine, 1 part of sodium chloride, 0.1 part of polyglutamic acid, 4 parts of beta-glucan and 40 parts of physiological saline. Preferably, the cellular fibronectin concentration is 1.2 mg/mL.

Example 3

This example provides a cellular fibronectin preparation for use in the method of anti-aging regenerative repair of cellular fibronectin. The cellular fibronectin preparation is prepared from the following raw materials in parts by weight: 0.6 part of cellular fibronectin, 4 parts of glycine, 2 parts of sodium chloride, 0.3 part of polyglutamic acid, 6 parts of beta-glucan and 100 parts of normal saline. Preferably, the cellular fibronectin concentration is 1.4 mg/mL.

Example 4

This example provides a cellular fibronectin preparation for use in the method of anti-aging regenerative repair of cellular fibronectin. The cellular fibronectin preparation is prepared from the following raw materials in parts by weight: 0.5 part of cellular fibronectin, 3 parts of glycine, 1.5 parts of sodium chloride, 0.2 part of polyglutamic acid, 5 parts of beta-glucan and 70 parts of physiological saline. Preferably, the cellular fibronectin concentration is 1.3 mg/mL.

The cellular fibronectin preparations provided in examples 2 to 4 were prepared from cellular fibronectin, glycine, sodium chloride, polyglutamic acid, β -glucan, and physiological saline, and the preparations of the present application had good anti-aging and regeneration effects due to the combination of cellular fibronectin, polyglutamic acid, and β -glucan.

Example 5

This example provides a method for preparing a cellular fibronectin preparation, comprising the steps of:

step one, dividing normal saline into a first solution and a second solution according to the volume ratio of 4: 1; putting the first solution and the second solution into two reaction kettles, and adding cellular fibronectin into the first solution for later use;

step two, sequentially adding glycine, sodium chloride and polyglutamic acid into the first solution added with cellular fibronectin in the step one to prepare a mixture for later use;

and step three, adding the second solution and the beta-glucan into the mixture prepared in the step two at the same time, stirring while adding, and uniformly stirring to prepare the cellular fibronectin preparation.

Further, the first step, the second step and the third step are carried out at the temperature of 15-30 ℃.

Example 6

The embodiment provides a preparation method of beta-glucan, which comprises the following steps: puffing grains to obtain puffed material, mixing puffed material with water, adjusting pH of the extractive solution, and performing solid-liquid separation; adding amylase into the separated liquid, and separating, purifying and concentrating after saccharification reaction to obtain the beta-glucan. Mixing the puffed material with water at a mass ratio of 1: 10-20, adjusting pH to 6-14, and extracting at 30-100 deg.C for 0.5-10 hr.

The preparation methods of example 5 and example 6 can fully integrate the efficacy of each component and fully retain the effective ingredients of each component.

Example 7

The embodiment provides application of a cell engineering transdermal nutrition-based fibronectin anti-aging regeneration repair method in anti-aging.

Finally, it should be noted that: the above embodiments are only used to illustrate the technical solution of the present invention, and not to limit the same; while the invention has been described in detail and with reference to the foregoing embodiments, it will be understood by those skilled in the art that: the technical solutions described in the foregoing embodiments may still be modified, or some or all of the technical features may be equivalently replaced; and the modifications or the substitutions do not make the essence of the corresponding technical solutions depart from the scope of the technical solutions of the embodiments of the present invention.