阅读说明：本技术 一种重组人胰岛素或其类似物的前体的制备方法 (Preparation method of precursor of recombinant human insulin or analogue thereof ) 是由 赵亮亮 韩广杰 牛宁宁 李娜 刘衍伟 王宏伟 于 2019-05-23 设计创作，主要内容包括：一种重组人胰岛素或其类似物的前体的制备方法,包括a.菌体发酵,将发酵液进行连续流离心,收集清液；b.将步骤a中的清液经过中空纤维膜过滤,收集滤液；c.将步骤b中的滤液通过层析柱进行纯化。该方法具有步骤精简、易放大生产、无有机溶剂、收率高等优点,胰岛素前体纯度可高于90％,宿主细胞蛋白去除率高于90％,外源性DNA去除率达到89％及以上、低于0.1ng/mg。(A preparation method of precursor of recombinant human insulin or analogues thereof comprises a, fermenting thallus, carrying out continuous flow centrifugation on fermentation liquor, and collecting clear liquid; b. filtering the clear liquid in the step a through a hollow fiber membrane, and collecting filtrate; c. and (c) purifying the filtrate in the step b by a chromatographic column. The method has the advantages of simple steps, easy amplification production, no organic solvent, high yield and the like, the purity of the insulin precursor can be higher than 90%, the removal rate of host cell protein is higher than 90%, and the removal rate of exogenous DNA reaches 89% or more and is lower than 0.1 ng/mg.)