阅读说明：本技术 一种治疗肝损伤的中药组合物及其应用 (Traditional Chinese medicine composition for treating liver injury and application thereof ) 是由 兰东强 严思奇 于 2020-04-03 设计创作，主要内容包括：本发明涉及中药组合物,具体说是一种治疗肝损伤的中药组合物及其应用,其中组合物其按质量份数由以下组分制成：丹参10-20份、麸炒柴胡5-15份、大青叶10-20份、当归5-15份、龙胆5-15份、五味子5-15份、甘草5-15份。在本发明的上述组合物中,以麸炒柴胡为君药,龙胆为臣药,大青叶、五味子、当归、丹参为佐助药,甘草为使药,以解郁为先,安全、可靠、有效、无毒副作用,对治疗肝胆湿热型的肝损伤效果明显,如药物性肝损伤或其他原因导致的肝损伤(脂肪肝、病毒性肝炎等)类之类的肝胆湿热型的肝损伤。(The invention relates to a traditional Chinese medicine composition, in particular to a traditional Chinese medicine composition for treating liver injury and application thereof, wherein the composition is prepared from the following components in parts by mass: 10-20 parts of salvia miltiorrhiza, 5-15 parts of radix bupleuri stir-fried with bran, 10-20 parts of folium isatidis, 5-15 parts of angelica sinensis, 5-15 parts of gentian, 5-15 parts of schisandra chinensis and 5-15 parts of liquorice. In the composition, the radix bupleuri stir-fried with bran is taken as a monarch drug, the gentian is taken as a ministerial drug, the folium isatidis, the schisandra chinensis, the angelica sinensis and the salvia miltiorrhiza are taken as adjuvant drugs, and the liquorice is taken as a conductant drug, so that the depression is relieved, the composition is safe, reliable, effective and free of toxic and side effects, and has an obvious effect of treating liver injury of liver and gallbladder damp-heat type, such as drug-induced liver injury or liver injury (fatty liver, viral hepatitis and the like) of liver and gallbladder damp-heat type caused by other reasons.)

1. A traditional Chinese medicine composition for treating liver injury is characterized by being prepared from the following components in parts by mass: 10-20 parts of salvia miltiorrhiza, 5-15 parts of radix bupleuri stir-fried with bran, 10-20 parts of folium isatidis, 5-15 parts of angelica sinensis, 5-15 parts of gentian, 5-15 parts of schisandra chinensis and 5-15 parts of liquorice.

2. The traditional Chinese medicine composition for treating liver injury according to claim 1, which is characterized by comprising the following components in parts by mass: 15 parts of salvia miltiorrhiza, 10 parts of radix bupleuri stir-fried with bran, 15 parts of folium isatidis, 10 parts of angelica sinensis, 10 parts of gentian, 10 parts of schisandra chinensis and 10 parts of liquorice.

3. The use of the Chinese medicinal composition for treating liver injury according to claim 1 or 2, which is characterized in that: the traditional Chinese medicine composition is applied to preparing the medicine for treating liver injury caused by dampness-heat of liver and gallbladder.

4. The use of the Chinese medicinal composition for treating liver injury according to claim 3, wherein: the traditional Chinese medicine composition is applied to preparing medicines for treating drug-induced liver injury.

5. The use of the Chinese medicinal composition for treating liver injury according to claim 3, wherein: the traditional Chinese medicine composition is applied to preparing the medicine for treating the fatty liver.

6. The use of the Chinese medicinal composition for treating liver injury according to claim 3, wherein: the traditional Chinese medicine composition is applied to preparing a medicine for treating viral hepatitis.

Technical Field

The invention relates to a traditional Chinese medicine composition, in particular to a traditional Chinese medicine composition for treating liver injury and application thereof.

Background

The symptoms of liver and gallbladder damp-heat type liver injury and tongue coating pulse condition caused by various reasons are as follows: bitter taste in the mouth, poor appetite, loose stool, irritability, nausea, vomiting, insomnia and other symptoms can be combined, and tongue coating and pulse conditions are as follows: a red tongue with yellow and greasy coating and a wiry and rapid pulse. The liver injury is caused by various reasons, such as fatty liver, long-term drinking, medicines, chemical substances, virus infection, long-term liver depression and qi stagnation, wherein the medicine liver injury is the most common clinically, the medicine liver injury belongs to the categories of hypochondriac pain, jaundice, accumulation and the like in the traditional Chinese medicine, and the direct disease is clinically mainly manifested by weakness, poor appetite, nausea, vomiting, epigastric or hypochondrial discomfort and the like due to the fact that the direct disease is infected by medicine toxin, and also can have fever, yellow skin and sclera, skin itch, dark yellow urine and the like, a few related joint pain and rash, and the liver can have swelling or swelling pain. The pathogenesis of the liver disease refers to the dysfunction of the liver in smoothing flow of qi and transport, and the dysfunction of the spleen in transportation. At present, the treatment of liver injury mainly comprises the treatment of stopping liver injury drugs and combining liver protection, various drugs are various, but the number of truly effective drugs is small, and the side effect is large. Based on the concept of no prevention before disease in traditional Chinese medicine, research and development of safe and effective medicines for treating liver injury are problems to be solved urgently.

Disclosure of Invention

Aiming at the problems, the invention provides a traditional Chinese medicine composition capable of treating liver and gallbladder damp-heat type liver injury caused by various reasons and application thereof.

The technical scheme adopted by the invention for solving the technical problems is as follows: a traditional Chinese medicine composition for treating liver injury is prepared from the following components in parts by weight: 10-20 parts of salvia miltiorrhiza, 5-15 parts of radix bupleuri stir-fried with bran, 10-20 parts of folium isatidis, 5-15 parts of angelica sinensis, 5-15 parts of gentian, 5-15 parts of schisandra chinensis and 5-15 parts of liquorice.

Preferably, the components are calculated according to the following parts by mass: 15 parts of salvia miltiorrhiza, 10 parts of radix bupleuri stir-fried with bran, 15 parts of folium isatidis, 10 parts of angelica sinensis, 10 parts of gentian, 10 parts of schisandra chinensis and 10 parts of liquorice.

The invention also provides an application of the traditional Chinese medicine composition for treating liver injury, and the traditional Chinese medicine composition is applied to preparation of a medicine for treating liver injury caused by dampness-heat in liver and gallbladder.

Preferably, the traditional Chinese medicine composition is applied to preparation of a medicine for treating drug-induced liver injury.

Preferably, the traditional Chinese medicine composition is applied to preparation of a medicine for treating fatty liver.

Preferably, the traditional Chinese medicine composition is applied to preparation of a medicine for treating viral hepatitis.

In the composition, the radix bupleuri fried with bran is taken as a monarch drug, the gentian is taken as a ministerial drug, the folium isatidis, the schisandra chinensis, the angelica sinensis and the salvia miltiorrhiza are taken as adjuvant drugs, and the liquorice is taken as a conductant drug, so that the composition is safe, reliable, effective and free of toxic and side effects, and has an obvious effect of treating liver injury of liver and gallbladder damp-heat type, such as drug-induced liver injury or liver injury (fatty liver, viral hepatitis and the like) of liver and gallbladder damp-heat type caused by other reasons.

Detailed Description

The present invention will now be described in detail with reference to the following examples, which are illustrative of the present invention and are not to be construed as limiting the invention.

The invention provides a traditional Chinese medicine composition for treating liver injury, which is prepared from the following components in parts by mass: 10-20 parts of salvia miltiorrhiza, 5-15 parts of radix bupleuri stir-fried with bran, 10-20 parts of folium isatidis, 5-15 parts of angelica sinensis, 5-15 parts of gentian, 5-15 parts of schisandra chinensis and 5-15 parts of liquorice. Because the liver is the rigid viscera and mainly has the functions of smoothing flow of qi, treating depression due to joy of sex and depression, and treating liver depression, the treatment takes depression relieving as the first step, and the bupleurum stir-fried with bran is the monarch drug in the formula, has pungent and dispersing flavor, soothes liver depression and regulates qi movement. Liver damage is usually caused by damp-heat, and the damp-heat is more serious and mild, and gentian root in the formula is used as a ministerial drug to clear heat and dry dampness, and excels in purging liver-fire and gallbladder-fire. The spleen and stomach are located in the middle energizer and are the pivot for qi movement to go up and down, and the middle-jiao gan Cao tonifies spleen and qi, relieves toxicity, relieves spasm and pain, and relieves property of the drugs. The kidney stores essence, the liver stores blood, and essence is transformed into blood, so there is isogenous with B and C, tonifying kidney and nourishing liver, so it uses Wu Wei Zi to astringe and arrest discharge, benefit qi and promote fluid production, tonify kidney and calm heart. Liver diseases are caused by stagnation of qi and blood stasis or blood stasis and qi stagnation, mainly adopts depression relieving method and blood stasis symptom, and adopts liver qi soothing and blood circulation promoting method, wherein salvia miltiorrhiza is used for promoting blood circulation to relieve pain, relieving restlessness and soothing nerves in the formula; dang Gui can activate blood and regulate menstruation, tonify blood, relax bowels and expel damp-heat from stool. The drug-induced liver injury is caused by direct toxicity of the drugs, and is accompanied by heat-clearing and detoxifying, while the dyers woad leaf in the formula has the effects of heat-clearing and detoxifying and cooling blood. Therefore, the traditional Chinese medicine composition has good curative effect on drug-induced liver injury. Meanwhile, in clinical practice, the composition of the invention is found to be as effective as liver injury caused by other reasons (such as fatty liver, viral hepatitis and the like). Therefore, the composition of the invention is applied to the preparation of medicines for treating drug-induced liver injury, fatty liver, viral hepatitis and the like, and has good treatment effect.

Preferably, the components are calculated according to the following parts by mass: 15 parts of salvia miltiorrhiza, 10 parts of radix bupleuri stir-fried with bran, 15 parts of folium isatidis, 10 parts of angelica sinensis, 10 parts of gentian, 10 parts of schisandra chinensis and 10 parts of liquorice.

Wherein:

dan Shen is bitter in flavor and slightly warm in nature, enters heart and liver meridians, and can activate blood, remove stasis and alleviate pain, so it is indicated for mass, abdominal mass, blood stasis and abdominal pain. The principle of red sage root for curing persistent and chronic hepatitis is that it can expand peripheral blood vessel, reduce portal vein pressure, improve blood circulation in liver and increase the supply of nutrients and oxygen to liver cell. Salvia miltiorrhiza can activate blood and remove stasis, and has a certain effect on eliminating hyperplasia of liver fibrous connective tissue.

Folium isatidis, bitter and cold in nature and flavor, enters liver, heart, stomach and spleen channels, has the effects of clearing heat and removing toxicity, cooling blood and removing ecchymoses, is mainly used for treating fever with high fever and polydipsia, coma, macula, hematemesis, epistaxis, jaundice and the like, has the effect of treating chronic granulocytic leukemia, for example, indirubin has a high inhibition rate on L7212 leukemia mice, and is compared with other anticancer drugs observed from animal experiments. The dyers woad leaf has the function of cholagogue, for example, the dyers woad leaf has the function of obviously increasing bile secretion for dogs, and the dyers woad leaf extract can increase the activity of mouse microsome CYP2E1 enzyme, but has no influence on the transcription level.

Chinese angelica, with sweet, pungent, bitter and warm nature and flavor, enters liver, heart and spleen channels, has the functions of enriching blood, promoting blood circulation, regulating menstruation, relieving pain, moistening dryness and lubricating intestines, and is mainly used for treating blood deficiency syndromes, irregular menstruation, amenorrhea, dysmenorrhea, abdominal mass accumulation, metrorrhagia, deficiency cold and abdominal pain and the like. Radix Angelicae sinensis has liver protecting effect, such as liver injury caused by acute carbon tetrachloride poisoning of mice. The radix Angelicae sinensis injection can prevent rat hepatic glycogen reduction caused by D-galactosamine, and protect activity of ATP enzyme, glucose-6-phosphatase, and 5-nucleotidase of cells. For normal animals, angelica does not affect the glycogen content and the utilization of glucose by the liver, but can enhance the endogenous respiration. Ip 25% Chinese angelica injection (10ml/kg body weight) for white rats has certain protection effect on hepatic cell nucleus damage caused by D-galactosamine.

Radix bupleuri, bitter in property, pungent and slightly cold in property, enters liver and gallbladder meridians, has the functions of relieving exterior syndrome, abating fever, soothing liver, relieving depression and lifting yang qi, and is mainly used for treating exogenous fever, alternating cold and heat, malaria, liver depression and hypochondriac pain and the like. The water extract and decoction (1: 20) of bupleuri radix can increase total bile output and bile salt content of dog. The crude saikosaponin is orally administered at a dose of 500mg/kg daily for 3 days, and can restore liver function of rats with carbon tetrachloride liver injury, and also can restore liver function and tissue injury caused by galactose. Experiments on mouse liver injury caused by carbon tetrachloride show that the saikosaponin a has the effects of protecting liver cell injury and promoting lipid metabolism in liver. Bupleuri radix has anti-tumor effect, such as saikosaponin d inhibiting tumor growth on mouse ehrlich ascites carcinoma by intragastric or intraperitoneal injection, and can significantly prolong animal survival time. Preparing Tumor Necrosis Factor (TNF) with anticancer effect from bupleuri radix and New Zealand white rabbit, and treating hepatocarcinoma cell as target cell to cause cancer cell necrosis and lysis; the same experiment was carried out using Hela cells and lung gland cells, and the same results were obtained.

Gentiana scabra Bunge, bitter and cold in nature and entering liver and gallbladder meridians, has the effects of clearing heat and drying dampness, purging liver fire and arresting convulsion, and is mainly used for treating damp-heat jaundice, stranguria with urine, yin swelling and pruritus, damp-heat leukorrhagia, head swelling and headache due to excessive fire of liver and gallbladder, conjunctival congestion and swelling pain, deafness and swelling, hypochondriac pain and bitter taste. Radix Gentianae has effects of promoting bile flow and protecting liver, and can significantly increase bile flow when administered 50g/kg radix Gentianae injection or 4.5g/kg intravenous injection to healthy dog in duodenum of healthy and liver-damaged mouse. It can be seen from the curve of bile secretion flow in dogs that two peaks appear at 5 and 20 minutes of administration. Gentiopicrin has protective effect on mouse liver acute injury model caused by carbon tetrachloride and D-galactosamine (Galn), and can relieve liver necrosis and liver cell pathological change degree of administration group animals, and resist hepatic cell glycogen synthesis disorder caused by carbon tetrachloride. 25g/kg of gentian injection is injected subcutaneously, and the content of serum bilirubin can be obviously reduced for a mouse experimental jaundice model (hyperbilirubinemia and cholestasis) caused by alpha-naphthalene isothiocyanate.

The schisandra chinensis is sour and warm in nature and taste, enters lung, heart and kidney channels, has the functions of astringing, arresting discharge, tonifying qi, promoting the production of body fluid, calming the heart and soothing the nerves, and is mainly used for treating cough and asthma due to deficiency, nocturnal emission, enuresis, chronic diarrhea, spontaneous perspiration and night sweat, body fluid deficiency and thirst, palpitation and insomnia and the like. Wu Wei Zi is used for tonics and unprocessed for cough. Schisandra chinensis is sour and salty in property, enters liver to tonify kidney, pungent and bitter in property, enters heart to tonify lung, and sweet in property, enters middle energizer to benefit spleen and stomach. Schisandrin B has an induction effect on drug enzymes, and is a drug enzyme inducer. The function of the drug enzyme is mainly biological transformation. P-450 is one of the important components in the pharmazyme system. The key role is played in the terminal transformation process of exogenous foreign matters such as steroids and lipid decomposition products and the like entering the body, such as drugs and poisons, and the drug enzyme induction is often accompanied by liver enlargement, and the two are coupled phenomena. Therefore, the mechanism of the anti-liver injury and detoxification of schisandrin B and the problem of liver enlargement caused by schisandrin B are mainly due to the effect of inducing drug enzymes.

Licorice root, radix Glycyrrhizae is sweet and mild in nature and taste. It has effects in invigorating qi, strengthening middle warmer, relieving pain, moistening lung, relieving cough, clearing pathogenic fire, removing toxic substances, and regulating functions of the above medicines. Glycyrrhizin can significantly prevent increase of glutamic-pyruvic transaminase of rat with carbon tetrachloride poisoning, and can reduce accumulation of triglyceride in liver. As can be seen from the pathological histological observation, the liver injury of the rats treated by glycyrrhizin and glycyrrhetinic acid is lighter than that of the control group. Histochemical observation shows that the liver glycogen of the white rat treated by the glycyrrhetinic acid is obviously increased, and the detection rate of the serum fetal alpha globulin of the glycyrrhizin and the glycyrrhetinic acid is higher than that of a control group. Suggesting that the two components have no collagen dissolution and reabsorption effects. In an animal model P of liver damage caused by carbon tetrachloride, glycyrrhizin and glycyrrhetinic acid have strong inhibition effect on liver damage.

The invention adopts bupleurum, salvia miltiorrhiza and schisandra chinensis, can obviously reduce drug-induced damage caused by CCl4 and the liver cell apoptosis rate of rats in a rat model with liver cirrhosis, and can block the degeneration of liver cells and the collagen fiber proliferation, thereby blocking the action of CCl4 for inducing rat liver cirrhosis, wherein the action is related to the inhibition of liver parenchymal activation, the reduction of cytokine release, the reduction of TIMP-1 wall released by lipid storage cells, the acceleration of collagen fiber decomposition and the reduction of liver cell apoptosis number. The radix bupleuri, the salvia miltiorrhiza and the schisandra chinensis can also obviously reduce serum glutamic-pyruvic transaminase of mice (CCl4+ D-GlaN) in two liver injury mouse models, can obviously increase serum and liver tissue CHE (cholinesterase), has obvious clearing effect on GSH (glutathione) accumulation caused by CCl4, and prompts that the synthesis and metabolism of glutathione peroxidase tend to be normal, thereby being beneficial to strengthening the detoxification function in the liver. Simultaneously, the MDA (malondialdehyde) level can be reduced, and the MDA and the malondialdehyde are prompted to improve the stress capability of the antioxidant defense system of the organism. (CCl4 model is similar to drug-induced liver injury model, D-GlaN model is similar to viral hepatitis-induced liver injury model).

The radix bupleuri and the salvia miltiorrhiza can effectively repair the damage of the liver pathological tissue, enhance the regeneration of liver cells, inhibit the formation of false lobules, protect the liver cells, relieve the malignant development of the tissue cells, and have good prognosis. The radix bupleuri and the schisandra chinensis can obviously reduce the serum ALT, AST, TG and MAO of rats with hepatic fibrosis; pathological tissue examination results show that the extracts of the Chinese thorowax root and the Chinese magnoliavine fruit can recover damaged liver cells and strengthen the regeneration function of the liver cells. Therefore, the bupleurum and schisandra extract has the functions of protecting and repairing damaged or injured liver cells, eliminating gallbladder stagnation and preventing the fatty degeneration of the liver cells. In addition, chai Hu has the action of soothing liver and gallbladder, so all herbs enter liver and gallbladder meridians. The radix bupleuri can reduce the intake of relevant medicines (such as gentian) by lung, kidney, spleen and heart, reduce the toxicity of the medicines to the organs, improve the distribution of other medicines in the liver and increase the relative comprehensive targeting efficiency of the liver of the prescription.

See the examples below for details:

implementation 1: taking 10g of salvia miltiorrhiza, 5g of radix bupleuri stir-fried with bran, 10g of folium isatidis, 5g of angelica sinensis, 5g of gentian, 5g of schisandra chinensis and 5g of liquorice, and decocting the traditional Chinese medicine components with water to obtain decoction for administration.