一种二聚体二氢杨梅素及其制备方法和作为自由基清除剂的用途
阅读说明：本技术 一种二聚体二氢杨梅素及其制备方法和作为自由基清除剂的用途 (Dimer dihydromyricetin, preparation method thereof and application of dimer dihydromyricetin as free radical scavenger ) 是由 张友胜 秦亚茹 张业辉 焦文娟 于 2020-03-09 设计创作，主要内容包括：本发明属于天然产物应用领域,公开了一种二聚体二氢杨梅素及其制备方法和作为自由基清除剂的用途。该二聚体二氢杨梅素具有如下式(Ⅰ)所示结构。制备方法包括步骤：二氢杨梅素溶于二甲基亚砜溶液中制成0.5-5.0mol/L的DMY储备液；用磷酸盐缓冲液将DMY储备液稀释至0.01-1.0mol/L；稀释液置于37℃恒温生化培养箱中温育72h-140h；浓缩,加入甲醇,去掉溶液中析出的磷酸盐；重复操作2-3次；加入乙酸乙酯,萃取去掉二甲基亚砜；浓缩、干燥得二聚体二氢杨梅素混合物；应用制备型HPLC对二聚体二氢杨梅素混合物进行制备,以二聚体二氢杨梅素为目标产物,得到纯度超过99％的二聚体二氢杨梅素。<Image he="645" wi="571" file="DDA0002404805530000011.GIF" imgContent="drawing" imgFormat="GIF" orientation="portrait" inline="no"></Image>(The invention belongs to the field of natural product application, and discloses a dimer dihydromyricetin, a preparation method thereof and an application of the dimer dihydromyricetin as a free radical scavenger, wherein the dimer dihydromyricetin has a structure shown in the following formula (I), and the preparation method comprises the steps of dissolving the dihydromyricetin in a dimethyl sulfoxide solution to prepare a DMY stock solution with the concentration of 0.5-5.0 mol/L molThe method comprises the steps of diluting DMY stock solution to 0.01-1.0 mol/L with phosphate buffer solution, placing the diluted solution in a constant-temperature biochemical incubator at 37 ℃ for incubation for 72-140 h, concentrating, adding methanol to remove phosphate separated out from the solution, repeating the operation for 2-3 times, adding ethyl acetate to extract and remove dimethyl sulfoxide, concentrating and drying to obtain a dimer dihydromyricetin mixture, and preparing the dimer dihydromyricetin mixture by using preparative HP L C to obtain the dimer dihydromyricetin with the purity of more than 99% by taking the dimer dihydromyricetin as a target product.)
技术领域
本发明属于天然产物应用领域，特别涉及一种二聚体二氢杨梅素及其制备方法和作为自由基清除剂的用途。
背景技术
二氢杨梅素为葡萄科蛇葡萄属中显齿蛇葡萄植物(藤茶)的主要活性成分，具有清除自由基、抗氧化、抗血栓、消炎、抗肿瘤等多种奇特功效，对人类的呼吸系统、消化系统、心血管系统、神经系统、免疫系统和内分泌系统等功能性疾病的预防和治疗均有重要意义。
二聚体二氢杨梅素是由2分子二氢杨梅素通过化学键聚合而成的一种稳定的化合物，对于其制备方法和用途，目前没有研究报道。