阅读说明：本技术 酶法合成寡核苷酸的方法和体系 (Method and system for enzymatic synthesis of oligonucleotides ) 是由 肯德尔·霍夫 米歇尔·哈尔佩因 詹卡洛·加尔巴尼亚蒂 周巍 于 2018-10-01 设计创作，主要内容包括：本发明涉及在聚合酶存在下由单链固定化引物酶法合成寡核苷酸的方法、过程和系统。利用本发明的方法,在脱氧核糖核苷酸三磷酸或核糖核苷酸三磷酸存在下,可从单链固定化引物酶法合成单链寡核苷酸。双脱氧核糖核苷酸三磷酸酯、带有可逆终止子的脱氧核糖核苷酸三磷酸酯或带有可逆终止子的核糖核苷酸三磷酸酯可以酶法添加到引物或其延伸产物的末端。根据本发明的方法,单链引物可与模板结合,所形成的双链结构可使聚合酶在3’端延伸引物。(The present invention relates to methods, processes and systems for the enzymatic synthesis of oligonucleotides from single-stranded immobilized primers in the presence of a polymerase. Using the method of the present invention, a single-stranded oligonucleotide can be enzymatically synthesized from a single-stranded immobilized primer in the presence of deoxyribonucleotide triphosphate or ribonucleotide triphosphate. A dideoxyribonucleotide triphosphate, a deoxyribonucleotide triphosphate with a reversible terminator or a ribonucleotide triphosphate with a reversible terminator may be enzymatically added to the end of a primer or its extension product. According to the method of the present invention, a single-stranded primer can be bound to a template, and the double-stranded structure formed allows a polymerase to extend the primer at the 3' end.)