阅读说明：本技术 一种短毛木耳及其培养方法和培养基 (Auricularia polytricha and culture method and culture medium thereof ) 是由 祁亮亮 郎宁 蓝桃菊 吴小建 李俐颖 陈振妮 叶建强 霍秀娟 林艳金 赵智勇 于 2019-08-06 设计创作，主要内容包括：本发明公开了一种短毛木耳及其培养方法和培养基,所述短毛木耳的保藏号为“CGMCC No.17779”。本发明公开了一种新品种的短毛木耳,并且明确其最佳的生长条件,为野生木耳的驯化、栽培及育种等提供了材料和依据。(The invention discloses auricularia polytricha and a culture method and a culture medium thereof, wherein the preservation number of the auricularia polytricha is CGMCC No. 17779. The invention discloses a new variety of auricularia polytricha, and defines the optimal growth conditions thereof, thereby providing materials and basis for domestication, cultivation, breeding and the like of wild auricularia polytricha.)

1. The auricularia polytricha is characterized in that the preservation number of the auricularia polytricha is CGMCC No. 17779.

2. The method of culturing Auricularia polytricha according to claim 1, wherein the culture temperature of Auricularia polytricha is 20-30 ℃.

3. The method for culturing Auricularia polytricha according to claim 2, wherein the Auricularia polytricha is cultured using malt extract as a carbon source.

4. The method of culturing Auricularia polytricha according to claim 2, wherein the Auricularia polytricha is cultured with peptone as a nitrogen source.

5. The bridge floor monitoring device of claim 1, wherein the Auricularia polytricha is cultured at a pH of 6.

6. The culture medium of Auricularia polytricha according to claim 1, comprising potato, glucose, agar, a carbon source, a nitrogen source and water; the mass ratio of the potatoes, the glucose, the agar, the carbon source, the nitrogen source and the water is 200: 20: 20: 20: 20: 1000, parts by weight; the pH of the medium was 6.

7. The culture medium of Auricularia polytricha according to claim 6, wherein the carbon source is malt extract powder; the nitrogen source is peptone.

Technical Field

The invention belongs to the field of biology, and particularly relates to auricularia polytricha and a culture method and a culture medium thereof.

Background

Auricularia fungi belong to Basidiomycota of Basidiomycota, Agaricales, Auriculariales, Auriculariaceae, most of the fungi grow on broad-leaved trees, fallen woods, dead branches, stumps or rotten woods, and a few of the fungi also grow on conifers, belong to an important group of wood rotting fungi, and play an important material circulation role in forest ecosystems. Auricularia (Auricularia) is a wood fungus widely distributed in the world, and the edible fungus of Auricularia not only has high nutritive value and medicinal value, but also has low calorie, and is a healthy and delicious edible and medicinal fungus. The Auricularia has effects of moistening lung, reducing weight, cleaning intestine and stomach, and removing toxic substances in vivo.

Guangxi karst landform is widely developed and spread in various regions to form strange karst landform, which constitutes one of the main characteristics of Guangxi landform. The excellent geographical position and the complex and diversified landforms provide favorable environment for the development of biological diversity in Guangxi, and also create abundant and diversified wild agaric germplasm resources in Guangxi, and agaric varieties in many Guangxi areas are not found at present.

Disclosure of Invention

In order to solve the problems, the invention discloses auricularia polytricha and a culture method and a culture medium thereof. The invention discloses a new variety of auricularia polytricha, and defines the optimal growth conditions thereof, and provides materials and basis for domestication, cultivation, breeding and the like of wild auricularia polytricha.

In order to achieve the purpose, the technical scheme of the invention is as follows:

the preservation number of the auricularia polytricha is CGMCC No. 17779.

In a further improvement, the culture temperature of the auricularia polytricha is 20-30 ℃.

In a further improvement, during the culture of the auricularia polytricha, malt extract powder is used as a carbon source.

In a further improvement, peptone is used as a nitrogen source during the culture of the auricularia polytricha.

In a further improvement, the pH value of the auricularia polytricha during culture is 6.

Further improvements include potatoes, glucose, agar, carbon sources, nitrogen sources and water; the mass ratio of the potatoes, the glucose, the agar, the carbon source, the nitrogen source and the water is 200: 20:20: 20: 20: 1000, parts by weight; the pH of the medium was 6.

In a further improvement, the carbon source is malt extract powder; the nitrogen source is peptone.

Drawings

FIG. 1 is a photograph of WSW-5630 Auricularia polytricha;

FIG. 2 is a hyphal pattern of WSW-5630 Auricularia polytricha on different carbon source media;

FIG. 3 is a graph of the growth rate of WSW-5630 Auricularia polytricha on different carbon source media;

FIG. 4 is a hyphal pattern of WSW-5630 Auricularia polytricha on different nitrogen source media;

FIG. 5 is a graph of the growth rate of WSW-5630 Auricularia polytricha on different nitrogen source media;

FIG. 6 is a mycelial map of WSW-5630 Auricularia polytricha on media of different pH;

FIG. 7 is a graph of the growth rate of WSW-5630 Auricularia polytricha on media of different pH;

FIG. 8 is a hyphal pattern of WSW-5630 Auricularia polytricha cultured at different temperatures;

FIG. 9 is a graph showing the growth rate of WSW-5630 Auricularia polytricha cultured at different temperatures.

FIG. 10 is a phylogenetic tree of WSW-5630 Auricularia polytricha and other Auricularia species.

Detailed Description

The invention is further explained with reference to the drawings and the embodiments.