阅读说明：本技术 一种枯茗醛/双精氨酸复合抗菌剂及制备方法和应用 (Cuminaldehyde/double-arginine composite antibacterial agent and preparation method and application thereof ) 是由 林琳 廖雪 崔海英 戴锦铭 于 2019-08-30 设计创作，主要内容包括：本发明属于食品抗菌剂制备领域,具体涉及一种枯茗醛/双精氨酸复合抗菌剂及制备方法和应用。本发明将双精氨酸与枯茗醛、乙醇、无菌蒸馏水按照一定比例混合制备枯茗醛/双精氨酸复合抗菌剂,不仅能够降低抗菌剂的制备成本,而且可提高抗菌剂的抗菌性能。该种新型复合抗菌剂能有效杀死各种食源性病原菌,如大肠杆菌和耐甲氧西林金黄色葡萄球菌等。与单独的双精氨酸(枯茗醛)相比,复合抗菌剂在水果蔬菜及肉类的应用中具有良好的抗菌效果。(The invention belongs to the field of preparation of food antibacterial agents, and particularly relates to a cuminaldehyde/double-arginine composite antibacterial agent, and a preparation method and application thereof. According to the invention, the double arginine, the cuminaldehyde, the ethanol and the sterile distilled water are mixed according to a certain proportion to prepare the cuminaldehyde/double arginine composite antibacterial agent, so that the preparation cost of the antibacterial agent can be reduced, and the antibacterial performance of the antibacterial agent can be improved. The novel composite antibacterial agent can effectively kill various food-borne pathogenic bacteria, such as escherichia coli, methicillin-resistant staphylococcus aureus and the like. Compared with single-component di-arginine (cuminaldehyde), the composite antibacterial agent has good antibacterial effect in the application of fruits, vegetables and meat.)

1. The cuminaldehyde/double-arginine composite antibacterial agent is characterized in that: the composite antibacterial agent comprises, by mass, 0.05% -0.2% of cuminaldehyde, 0.5% -1% of ethanol, 0.2% -1% of double arginine and the balance of sterile distilled water.

2. The method for preparing the composite antibacterial agent of cuminaldehyde/diarginine as claimed in claim 1, wherein the composite antibacterial agent is obtained by mixing diarginine, cuminaldehyde, ethanol and sterile distilled water in proportion, and dissolving them by magnetic stirring.

3. The method for preparing the cuminaldehyde/diarginine complex antibacterial agent according to claim 1, wherein the magnetic stirring condition is that the cuminaldehyde/diarginine complex antibacterial agent is fully dissolved after stirring for 1 hour at 25 r/min.

4. The use of the composite antimicrobial agent of cuminaldehyde/arginines according to claim 1, wherein the composite antimicrobial agent is used for storage and preservation of fruits, vegetables and meats.

Technical Field

The invention belongs to the field of preparation of food antibacterial agents, and particularly relates to a cuminaldehyde/double-arginine composite antibacterial agent, and a preparation method and application thereof.

Background

The existing antibacterial agents are various, and most of the existing antibacterial agents use organic antibacterial agents with certain toxicity, such as hypochlorous acid, vanillin and glutaraldehyde compounds. Under the background, the field of food antibacterial agent preparation urgently needs an antibacterial agent which is safe, non-toxic and has a broad-spectrum antibacterial effect.

The double arginine is one of the smallest biological self-assembly materials and is formed by dehydrating and condensing two arginines. At isoelectric point in the form of a zwitterion, i.e. -NH-at one end₃ ⁺One end is-COO^-And (3) arginine di-arginine. The self-assembly material can be self-assembled in a plurality of solvents, such as methanol, chloroform, water and the like, and is a short peptide which is widely researched, and is a biological self-assembly material with wide application prospect because the short peptide is composed of amino acid.

Cumin, also known as cumin celery or cumin, also known as cumin, belongs to the family of Umbelliferae and is an annual or biennial herb. Cuminum cyminum plants are native to Mediterranean sea and the northern African of the middle east, are distributed in India, Russia, North America and China at present, and the India is the world's largest Cuminum cyminum producing country. Cuminum celery in China only has 1 species, and is planted in Hexi corridor in Gansu and southern Xinjiang in Xinjiang. In addition, the cumin has the effects of regulating qi, stimulating appetite, warming the middle-jiao and spleen, promoting blood circulation, restoring consciousness, removing dampness, dispelling cold, dispelling wind, relieving pain and the like, can be used as an excitant, and is an important national medicine in some parts of China, such as Xinjiang. The cumin seeds are rich in essential oil, the content of the cumin seeds can reach 3% -4%, and the content of cuminic aldehyde and cuminic acid accounts for about 65% of the total content of the cumin essential oil.

The cuminaldehyde/double-arginine composite antibacterial agent with excellent antibacterial effect is prepared by combining the cuminaldehyde and the double-arginine, and has better application prospect and market value.

Disclosure of Invention

The invention aims to provide a cuminaldehyde/double-arginine composite antibacterial agent, a preparation method and application thereof in food.

The preparation method of the cuminaldehyde/double-arginine composite antibacterial agent comprises the steps of mixing double-arginine, cuminaldehyde, ethanol and sterile distilled water according to a certain proportion, and obtaining the composite antibacterial agent after magnetic stirring and dissolution.

The cuminaldehyde/double-arginine composite antibacterial agent comprises, by mass, 0.05% -0.2% of cuminaldehyde, 0.5% -1% of ethanol, 0.2% -1% of double-arginine and the balance of sterile distilled water.

The magnetic stirring condition is that the mixture is stirred for 1 hour at 25r/min to be fully dissolved.

The invention combines the double arginine and the cuminaldehyde to prepare the cuminaldehyde/double arginine composite antibacterial agent with excellent antibacterial effect. The invention has good sterilization effect which is as high as 99.999 percent, and can be applied to sterilization and fresh-keeping of most foods. The invention has no influence on the quality of food, and has great development prospect in the aspects of food antibiosis and fresh keeping, even cleaning and sterilization of medical appliances.

Drawings

FIG. 1 shows the antibacterial effect of the cuminaldehyde/arginin complex antibacterial agent on Escherichia coli.

FIG. 2 shows the antibacterial effect of the cuminaldehyde/arginin complex antibacterial agent against E.coli in cherry tomatoes.

FIG. 3 shows the antibacterial effect of the cuminaldehyde/arginin complex antibacterial agent against methicillin-resistant Staphylococcus aureus (MRSA).

FIG. 4 shows the antibacterial effect of the cuminaldehyde/arginin complex antibacterial agent against methicillin-resistant Staphylococcus aureus (MRSA) in pork.

N.d. indicates no detection.

Detailed Description

The following examples illustrate specific embodiments of the present invention, but the scope of the present invention is not limited thereto.