阅读说明：本技术 一种芹菜素的提取工艺 (Extraction process of apigenin ) 是由 杨成东 于 2018-05-29 设计创作，主要内容包括：本发明涉及一种芹菜素的提取工艺,具体涉及一种匀浆法与超声波相结合提取芹菜素的方法。其特点是,包括如下步骤：a.取芫花切成1~2cm的小段置于匀浆机内,加入提取溶剂,在匀浆机中混合匀浆；b.匀浆后的物料置于超声装置中,提取2h；c.微滤膜过滤,减压浓缩至原体积的1/4-6；d.上述提取物用乙醇溶液溶解,加入活性炭回流脱色,脱色液浓缩后用乙醚萃取,萃取液浓缩结晶,即得芹菜素。本发明流程简单、快速、高效的优点,同时整个工艺有机溶剂使用量少,能够降低成本,具有良好的应用前景。(The invention relates to an extraction process of apigenin, in particular to a method for extracting apigenin by combining a homogenization method and ultrasonic waves. The method is characterized by comprising the following steps: a. cutting flos genkwa into small sections of 1-2 cm, placing the small sections in a homogenizer, adding an extraction solvent, and mixing and homogenizing in a homogenizer; b. placing the homogenized material in an ultrasonic device, and extracting for 2 h; c. filtering with microfiltration membrane, and concentrating under reduced pressure to 1/4-6 of original volume; d. dissolving the above extract with ethanol solution, adding active carbon, refluxing for decolorizing, concentrating decolorized solution, extracting with diethyl ether, concentrating the extractive solution, and crystallizing to obtain apigenin. The method has the advantages of simple flow, rapidness and high efficiency, and meanwhile, the whole process has small usage amount of organic solvent, can reduce cost and has good application prospect.)

1. The extraction process of apigenin is characterized by comprising the following steps:

a. Cutting flos Genkwa into 1-2 cm segments, placing in a homogenizer, adding 70-80% ethanol as extraction solvent, and mixing and homogenizing in a homogenizer;

b. placing the homogenized material in an ultrasonic device, and extracting for 2 h;

c. Filtering with microfiltration membrane, and concentrating under reduced pressure to 1/4-6 of original volume;

d. dissolving the above extract with ethanol solution, adding active carbon, refluxing for decolorizing, concentrating decolorized solution, extracting with diethyl ether, concentrating the extractive solution, and crystallizing to obtain apigenin.

2. the extraction process of apigenin according to claim 1, characterized in that the power of the homogenizer in step a is 160W, and the rotation speed of the homogenizer is 8000-12000 r/min.

3. The extraction process of apigenin according to claim 1, wherein the microfiltration membrane in step c is Al2O3, 0.2 μm.

Technical Field

the invention belongs to the technical field of plant extraction, relates to an apigenin extraction process, and particularly relates to a method for extracting apigenin by combining a homogenization method and ultrasonic waves.

background

The homogenate extraction refers to a method for extracting effective components from tissues by adding an extraction solvent to homogenate or grind the tissues. The method for extracting the effective components of the plants by using the homogenization method can directly place the materials in a homogenizer, mix the materials with an extraction solvent in a homogenizing device for homogenization, tear and crush the materials by mechanical and hydraulic shearing action, and synchronously carry out material crushing and effective component extraction, thereby achieving the purpose of quickly and intensively extracting the effective components of the plants. The method has the advantages of complete extraction, high efficiency, low energy consumption, etc. At present, no report on the method for extracting apigenin is found.

disclosure of Invention

The technical problem to be solved by the invention is as follows: aiming at the defects of the prior art, a process for extracting apigenin from lilac daphne flower bud is provided.

The technical scheme of the invention is carried out according to the following steps:

a. cutting flos Genkwa into 1-2 cm segments, placing in a homogenizer, adding 70-80% ethanol as extraction solvent, and mixing and homogenizing in a homogenizer;

b. placing the homogenized material in an ultrasonic device, and extracting for 2 h;

c. Filtering with microfiltration membrane, and concentrating under reduced pressure to 1/4-6 of original volume;

d. Dissolving the above extract with ethanol solution, adding active carbon, refluxing for decolorizing, concentrating decolorized solution, extracting with diethyl ether, concentrating the extractive solution, and crystallizing to obtain apigenin.

The power of the homogenizer in the step a is 160W, and the rotating speed of the homogenizer is 8000-12000 r/min.

The microfiltration membrane in the step c is Al2O3 of 0.2 mu m

the present invention will be further described with reference to specific embodiments, but the scope of the invention as claimed is not limited to the following embodiments.

the specific implementation mode is as follows: