阅读说明：本技术 通过使用捕获前的絮凝改善免疫球蛋白的亲和色谱 (Improved affinity chromatography of immunoglobulins by using flocculation prior to capture ) 是由 Z·凯西 Z·苏托 于 2020-03-25 设计创作，主要内容包括：本发明涉及用于从细胞培养收获物中纯化免疫球蛋白的新方法。这些方法的特征在于在初始捕获色谱步骤之前的清洗步骤。优选地,初始捕获色谱为亲和色谱,并且通过絮凝和过滤获得捕获前的澄清效果。通过使用此类预捕获的澄清步骤,实现了从捕获基质洗脱的免疫球蛋白的改进的质量。此外,细胞培养液的更澄清导致亲和色谱过程中的沉淀减少,由此增加了昂贵的亲和树脂的寿命。这是以大规模生产的免疫球蛋白纯化的重要改善。本发明进一步涉及捕获色谱下游的连续精制色谱和过滤步骤,产生高纯度的免疫球蛋白。(The present invention relates to a novel method for purifying immunoglobulins from cell culture harvests. These methods are characterized by a washing step prior to the initial capture chromatography step. Preferably, the initial capture chromatography is affinity chromatography and the clarification effect before capture is obtained by flocculation and filtration. By using such a pre-capture clarification step, an improved quality of the immunoglobulin eluted from the capture matrix is achieved. Furthermore, the clarification of the cell culture broth leads to a reduction of the precipitation during affinity chromatography, thereby increasing the lifetime of the expensive affinity resin. This is an important improvement in the purification of immunoglobulins produced on a large scale. The invention further relates to a continuous purification chromatography and filtration step downstream of the capture chromatography, resulting in high purity immunoglobulins.)