阅读说明：本技术 一种从显齿蛇葡萄叶中提取二氢杨梅素的方法 (Method for extracting dihydromyricetin from ampelopsis grossedentata leaves ) 是由 陆丽萍 于 2021-09-18 设计创作，主要内容包括：本发明公开了一种从显齿蛇葡萄叶中提取二氢杨梅素的方法,包括以下步骤：S1、采摘鲜叶,将其分割为小段,进行摊凉；S2、将摊凉叶采用蒸汽杀青方式进行杀青；S3、将得到的杀青叶进行一次揉捻,采用螺旋绞龙进行挤压揉捻；S4、将得到的揉捻叶进行一次发酵,在无氧发酵箱中发酵2小时；S5、将一次发酵的显赤蛇葡萄叶放入蒸笼进行蒸煮,对蒸汽进行冷凝,同时对蒸笼水进行收集；S6、将蒸煮后的叶片进行二次揉捻；S7、将二次揉捻后的叶片,进行二次发酵,发酵时间为24小时；S8、将二次发酵的叶片进行烘干,然后粉碎；S9、将粉碎后的叶片与溶剂混合,搅拌过滤,得到溶液；S10、将冷凝水、蒸笼水和溶液混合；S11、将混合液过滤,对混合液进行提取。(The invention discloses a method for extracting dihydromyricetin from ampelopsis grossedentata leaves, which comprises the following steps: s1, picking fresh leaves, dividing the fresh leaves into small segments, and spreading for cooling; s2, deactivating enzyme of the spread and cooled leaves by adopting a steam deactivation mode; s3, rolling the obtained enzyme-removed leaves for the first time, and extruding and rolling by adopting a spiral auger; s4, performing primary fermentation on the obtained twisted leaves, and fermenting for 2 hours in an anaerobic fermentation box; s5, steaming the primarily fermented red-snake grape leaves in a steamer, condensing steam, and collecting water in the steamer; s6, carrying out secondary rolling on the steamed leaves; s7, carrying out secondary fermentation on the twice-twisted leaves for 24 hours; s8, drying the twice fermented leaves, and then crushing; s9, mixing the crushed leaves with a solvent, stirring and filtering to obtain a solution; s10, mixing the condensed water, the steamer water and the solution; s11, filtering the mixed solution, and extracting the mixed solution.)

1. A method for extracting dihydromyricetin from Ampelopsis grossedentata leaves is characterized by comprising the following steps:

s1, picking fresh leaves of the red-brown ampelopsis, dividing the fresh leaves into small segments of 3-5cm, and forcibly spreading and cooling the small segments by using a fan to ensure that the fresh leaves lose 20 percent of water;

s2, de-enzyming the spread and cooled leaves obtained in the step S1, and de-enzyming the fresh dehydrated leaves in a steam de-enzyming mode;

s3, rolling the enzyme-removed leaves obtained in the step S2 for the first time, and extruding and rolling by adopting a spiral auger;

s4, performing primary fermentation on the twisted leaves obtained in the step S3, and fermenting for 2 hours in an anaerobic fermentation box;

s5, steaming the primarily fermented red-snake grape leaves in a steamer, condensing steam, and collecting water in the steamer;

s6, twisting the steamed red ampelopsis leaves for the second time;

s7, carrying out secondary fermentation on the red-snake grape leaves after secondary rolling for 24 hours;

s8, drying the red-snake grape leaves subjected to secondary fermentation, and crushing after drying;

s9, mixing the crushed red-striped ampelopsis leaves with a solvent, stirring and filtering to obtain a solution;

s10, mixing the condensed water, the steamer water and the solution, adding the solvent, stirring, and heating the mixed solution;

and S11, filtering the mixed solution, standing to obtain a solid mixture, and extracting the solid mixture.

2. The method of claim 1, wherein the extraction of dihydromyricetin from Ampelopsis grossedentata leaves comprises: the weight ratio of the condensed water to the steamer water to the solution is 1: 1: 1.

3. the method of claim 2, wherein the extraction of dihydromyricetin from Ampelopsis grossedentata leaves comprises: the solvent is one or the combination of methanol solution and ethanol solution.

4. The method of claim 3, wherein the extraction of dihydromyricetin from Ampelopsis grossedentata leaves comprises: the mixed solution comprises at least two times of filtration.

5. An apparatus for using the method for extracting dihydromyricetin from ampelopsis grossedentata leaves as claimed in claim 4, comprising a cooling device (1), a rolling device, a cooking device (3), a fermentation device (4), a drying device (5) and a crushing device (6), characterized in that: spread out cool device (1) and link to each other with cooking device (3) through first conveyer (71), it includes that thick twisting subassembly (21) and thin twisting subassembly (22) are kneaded to the device, thick twisting subassembly (21) sets up between fermenting installation (4) and cooking device (3), thin twisting subassembly (22) set up in fermenting installation (4), fermenting installation (4) link to each other with drying device (5) through second conveyer (72), drying device (5) link to each other with reducing mechanism (6), reducing mechanism (6) one end is provided with extraction element (8), cooking device (3) link to each other with extraction element (8).

6. The apparatus of claim 5, wherein: the rough twisting component (21) comprises an auger shaft (211) and a shell (212), the auger shaft (211) is arranged in the shell (212), and one end of the auger shaft (211) is connected with a driving motor.

7. The apparatus of claim 6, wherein: the extraction device (8) comprises an outer cylinder (811) and an inner cylinder (818), a cover plate (814) for closing an opening is rotatably connected to the inner cylinder (818), a solvent spray head (815) and a gas spray head (817) are fixed to the cover plate (814), a first screen (820) is arranged on the side wall of the inner cylinder (818), and a baffle plate (821) for closing the first screen (820) is vertically connected to the cover plate (814) in a sliding manner; the bottom of the outer cylinder (811) is provided with a second screen (826), the inner cylinder (818) is fixed with a cylinder (822), an output shaft of the cylinder (822) is fixed with a sealing plate (824) used for sealing the second screen (826), the bottom of the outer cylinder (811) is fixed with a heater (823), and the outer cylinder (811) is fixed with a liquid inlet spray head and a recoverer.

Technical Field

The invention relates to the technical field of dihydromyricetin extraction, in particular to a method for extracting dihydromyricetin from Ampelopsis grossedentata leaves.

Background

The ampelopsis grossedentata is a plant of ampelopsis of Vitaceae, is a special plant of China, contains 18 amino acids necessary for human bodies, 22 trace elements and more than or equal to 6 percent of flavonoid compounds as functional components, has the function of improving the immunity of the human bodies, and is particularly suitable for people with weak constitution and people with low immunity. The vine tea has unique function, wide application, no toxic side effect and suitability for both old and young, and can comprehensively enhance the body resistance, eliminate diseases, strengthen the body and prolong the life after being drunk for a long time. The vine tea as a health-care tea made of precious pure natural wild plants has unique taste and comprehensive efficacy which is incomparable with any tea. Ordinary tea can not be drunk at night and is easy to spoil, while the active flavone of the vine tea can kill bacteria and can be drunk after being soaked for one week. The Chinese and western medicines can not be taken together with common tea, and can not be dissolved by tea, and the vine tea can not be dissolved by taking together with the vine tea, and can assist the Chinese and western medicines to exert better effect. A major revolution called "tea" by plant experts. The longevity rattan and magic tea is prepared from Tujia people who have cold, inflammation, frequent micturition, burn, scald, acne and foot odor hundreds of years ago by oral administration and external application, and is a longevity rattan and magic tea which is prepared by a plurality of centenarians and has the average life of more than 83 years. The main active component dihydromyricetin of vine tea has various physiological activities such as liver protection, cancer resistance and the like, and currently, dihydromyricetin is used as a novel anticancer drug and is started to enter clinical tests. The ampelopsis grossedentata has high content of dihydromyricetin, and is not convenient to extract the dihydromyricetin by adopting the traditional equipment for fermentation. The dihydromyricetin in the vine tea is mainly prepared by fermentation, and the selection of a fermentation method and fermentation equipment is particularly important when the vine tea is prepared, so that quality problems can be caused by carelessness. The existing dihydromyricetin is extracted by directly adopting the vine tea after processing during the extraction process, partial dihydromyricetin loss can exist in the vine tea during the processing process, so that the extraction rate is not high, the existing extraction scheme is mature, and the application does not relate to the extraction step but aims to obtain higher extraction rate.

Disclosure of Invention

In order to solve the technical problems in the prior art, the following technical scheme is provided: a method for extracting dihydromyricetin from Ampelopsis grossedentata leaves comprises the following steps:

s1, picking fresh leaves of the red-brown ampelopsis, dividing the fresh leaves into small segments of 3-5cm, and forcibly spreading and cooling the small segments by using a fan to ensure that the fresh leaves lose 20 percent of water;

s2, de-enzyming the spread and cooled leaves obtained in the step S1, and de-enzyming the fresh dehydrated leaves in a steam de-enzyming mode;

s3, rolling the enzyme-removed leaves obtained in the step S2 for the first time, and extruding and rolling by adopting a spiral auger;

s4, performing primary fermentation on the twisted leaves obtained in the step S3, and fermenting for 2 hours in an anaerobic fermentation box;

s5, steaming the primarily fermented red-snake grape leaves in a steamer, condensing steam, and collecting water in the steamer;

s6, twisting the steamed red ampelopsis leaves for the second time;

s7, carrying out secondary fermentation on the red-snake grape leaves after secondary rolling for 24 hours;

s8, drying the red-snake grape leaves subjected to secondary fermentation, and crushing after drying;

s9, mixing the crushed red-striped ampelopsis leaves with a solvent, stirring and filtering to obtain a solution;

s10, mixing the condensed water, the steamer water and the solution, adding the solvent, stirring, and heating the mixed solution;

and S11, filtering the mixed solution, standing to obtain a solid mixture, and extracting the solid mixture.

As a further improvement of the above technical solution:

the weight ratio of the condensed water to the steamer water to the solution is 1: 1: 1.

the solvent is one or the combination of methanol solution and ethanol solution.

The mixed solution comprises at least two times of filtration.

The invention also discloses equipment for using the method for extracting dihydromyricetin from Ampelopsis grossedentata leaves as claimed in claim 4, which comprises a spreading and cooling device, a rolling device, a cooking device, a fermentation device, a drying device and a crushing device, wherein the spreading and cooling device is connected with the cooking device through a first conveyor, the rolling device comprises a coarse rolling component and a fine rolling component, the coarse rolling component is arranged between the fermentation device and the cooking device, the fine rolling component is arranged in the fermentation device, the fermentation device is connected with the drying device through a second conveyor, the drying device is connected with the crushing device, one end of the crushing device is provided with an extraction device, and the cooking device is connected with the extraction device.

As a further improvement of the above technical solution:

the rough twisting component comprises an auger shaft and a shell, the auger shaft is arranged in the shell, and one end of the auger shaft is connected with a driving motor.

The extraction device comprises an outer cylinder and an inner cylinder, a cover plate for sealing an opening is rotatably connected to the inner cylinder, a solvent spray nozzle and a gas spray nozzle are fixed on the cover plate, a first screen is arranged on the side wall of the inner cylinder, and a baffle for sealing the first screen is vertically and slidably connected to the cover plate; the bottom of urceolus is provided with the second screen cloth, be fixed with the cylinder on the inner tube, the output shaft of cylinder is fixed with the closing plate that is used for sealing the second screen cloth, the bottom of urceolus is fixed with the heater, be fixed with feed liquor shower nozzle and recoverer on the urceolus.

Compared with the prior art, the invention has the following beneficial effects:

the method for extracting the dihydromyricetin from the ampelopsis grossedentata leaves of the invention ensures that the dihydromyricetin in the ampelopsis grossedentata leaves can overflow fully through two times of fermentation and two times of cooking. The first fermentation activates 10-30% dihydromyricetin, and the dihydromyricetin is extracted from fresh leaves by cooking, the second fermentation can completely activate the residual dihydromyricetin, the two fermentation processes are favorable for extracting the dihydromyricetin, and the extraction rate of the dihydromyricetin is obviously improved.

Drawings

FIG. 1 is a schematic diagram of the apparatus of the present invention;

FIG. 2 is a schematic view of the structure of the extraction apparatus of the present invention;

FIG. 3 is a schematic view of the rough rolling assembly according to the present invention.

Reference numerals: 1. spreading and cooling the device; 3. a cooking device; 4. a fermentation device; 5. a drying device; 6. a crushing device; 71. a first transmitter; 72. a second transmitter; 8. an extraction device; 21. a rough rolling component; 211. a packing auger shaft; 212. a housing; 213. a drive motor; 22. a fine rolling assembly; 811. an outer cylinder; 813. a recoverer; 814. a cover plate; 815. a solvent spray head; 816. a motor; 817. a gas shower; 818. an inner barrel; 819. a liquid inlet spray head; 820. a first screen; 821. a baffle plate; 822. a cylinder; 823. a heater; 824. a closing plate; 826. a second screen.

Detailed Description

The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.

In the description of the present invention, it should be understood that the terms "front", "back", "left", "right", "up", "down", and the like indicate orientations or positional relationships based on those shown in the drawings, and are used merely for convenience in describing the present invention and simplifying the description, but do not indicate or imply that the devices or elements indicated by the terms must have specific orientations, be constructed and operated in specific orientations, and therefore, should not be construed as limiting the present invention.

As shown in fig. 1 to 3, the method for extracting dihydromyricetin from ampelopsis grossedentata leaves of the present invention comprises the following steps:

s1, picking fresh leaves of the red-striped ampelopsis, dividing the fresh leaves into 3-5cm segments, putting the segments into a spreading and cooling device 1, forcibly spreading and cooling the segments by using a fan to ensure that the fresh leaves lose 20 percent of water, wherein the spreading and cooling device 1 is mature in the current market, and the spreading and cooling device with higher sales is selected.

S2, conveying the spread and cooled leaves obtained in the step S1 into a cooking device 3 through a first conveyor 71 for enzyme deactivation, and performing enzyme deactivation in a steam enzyme deactivation mode; the choice of the first conveyor 71 is a routine measure for a person skilled in the art and is not exhaustive here.

S3, rolling the enzyme-removed leaves obtained in the step S2 for the first time, and extruding and rolling by adopting a spiral auger; the method is characterized in that the enzyme-deactivated leaves are conveyed by the coarse rolling component 21, and the enzyme-deactivated leaves are rolled by the auger shaft in the conveying process.

S4, performing primary fermentation on the twisted leaves obtained in the step S3, and fermenting for 2 hours in an anaerobic fermentation box; here, the twisted leaves conveyed by the rough twisting unit 21 are fermented in the fermentation device 4, so that the partial dihydromyricetin is easily overflowed.

S5, steaming the primarily fermented red-snake grape leaves in a steamer, condensing steam, and collecting water in the steamer; the method of transporting the fermented leaves from the fermentation device 4 back to the cooking device 3 for cooking without using a transporting device, and then obtaining dihydromyricetin produced by the primary fermentation, condensing and collecting the steamer water, and the cooking equipment are well known to those skilled in the art, and thus are not detailed herein.

S6, twisting the steamed red ampelopsis leaves for the second time; here, the leaves are transported from the steaming device 3 to the fermentation device 4 by the rough rolling assembly 21, and then the fine rolling assembly 22 is started to roll the leaves, wherein the rolling time is longer than that of one rolling time, and the leaves can be processed by a rolling method or device in the vine tea processing process.

S7, carrying out secondary fermentation on the red-snake grape leaves after secondary rolling for 24 hours; the fermentation process is completed, and the secondary fermentation can be performed in the fermentation device 4, wherein the fermentation time is long, so that the dihydromyricetin in the leaves can be fully overflowed.

S8, drying the red-snake grape leaves subjected to secondary fermentation, and crushing after drying; here, the fermented leaves are placed into a drying device 5 for drying, and dihydromyricetin overflowing in the drying process begins to crystallize on the surfaces of the leaves.

S9, mixing the crushed red-striped ampelopsis leaves with a solvent, stirring and filtering to obtain a solution; the method comprises the steps of putting dried and crystallized blades into a crushing device 6 for crushing and screening, wherein crushing and screening devices are conventional devices which are not detailed in the specification, mixing and stirring powder and a solvent, and filtering filter residues to obtain a prepared solution.

S10, mixing the condensed water, the steamer water and the solution, adding the solvent, stirring, and heating the mixed solution; the weight ratio of the condensed water, the steamer water and the preparation solution is 1: 1: 1, mixing, adding solvent, and extracting dihydromyricetin from the solution by conventional method, wherein the extraction method can be referred to CN202110352990.5 for separation process of dihydromyricetin from Ampelopsis Grossdentata. Collecting the first fermentation and steaming to improve extraction rate of dihydromyricetin. When the dihydromyricetin is extracted from the solution, the use of a catalyst can be reduced by adding the condensed water and the steamer water, and the working procedures are saved.

Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that changes, modifications, substitutions and alterations can be made in these embodiments without departing from the principles and spirit of the invention, the scope of which is defined in the appended claims and their equivalents.