阅读说明：本技术 用于生产3-羟基己二酸、α-氢化己二烯二酸和/或己二酸的基因修饰微生物以及该化学品的制造方法 (Genetically modified microorganisms for the production of 3-hydroxyadipic acid, alpha-hydrogenated adipic acid and/or adipic acid and methods for the production of such chemicals ) 是由 矶部匡平 河村健司 山田胜成 于 2020-05-08 设计创作，主要内容包括：本发明公开了能够以高产率生产3-羟基己二酸、α-氢化己二烯二酸和/或己二酸的基因修饰微生物以及利用该基因修饰微生物制造3-羟基己二酸、α-氢化己二烯二酸和/或己二酸的方法。该基因修饰微生物具有制造3-羟基己二酸、α-氢化己二烯二酸和/或己二酸的能力,并且使丙酮酸激酶的功能受损,强化了磷酸烯醇丙酮酸羧化激酶的活性和对将3-氧代己二酰-CoA还原生成3-羟基己二酰-CoA的反应进行催化的酶的活性。(Disclosed are a genetically modified microorganism capable of producing 3-hydroxyadipic acid, alpha-hydrogenated adipic acid and/or adipic acid in high yield, and a method for producing 3-hydroxyadipic acid, alpha-hydrogenated adipic acid and/or adipic acid using the genetically modified microorganism. The genetically modified microorganism has the ability to produce 3-hydroxyadipic acid, alpha-hydrohexadiene diacid and/or adipic acid and impaired pyruvate kinase function, enhancing the activity of phosphoenolpyruvate carboxylase and enzymes catalyzing the reduction of 3-oxoadipyl-CoA to 3-hydroxyadipyl-CoA.)

1. A genetically modified microorganism having the ability to produce 3-hydroxyadipate, alpha-hydrohexadiene-adipate and/or adipate and impaired pyruvate kinase function, having enhanced phosphoenolpyruvate carboxylase activity and enzymatic activity catalyzing the reduction of 3-oxoadipyl-CoA to 3-hydroxyadipyl-CoA.

2. The genetically modified microorganism of claim 1, further comprising a function impairment of phosphotransferase-like enzymes.

3. The genetically modified microorganism according to claim 1 or2, wherein,

the enzyme catalyzing the reaction of reducing the 3-oxoadipyl-CoA to 3-hydroxyadipyl-CoA is any one of the following (a) to (c):

(a) a polypeptide consisting of any one of the amino acid sequences of SEQ ID Nos. 1 to 7;

(b) a polypeptide which comprises an amino acid sequence obtained by substituting, deleting, inserting and/or adding 1 or more amino acids in any one of the amino acid sequences of SEQ ID Nos. 1 to 7 and has an enzymatic activity that catalyzes a reaction in which 3-oxoadipyl-CoA is reduced to 3-hydroxyadipyl-CoA;

(c) a polypeptide having a sequence homology of 70% or more with respect to any one of the amino acid sequences of SEQ ID Nos. 1 to 7, and having an enzymatic activity catalyzing a reaction in which 3-oxoadipyl-CoA is reduced to 3-hydroxyadipyl-CoA.

4. A process for the manufacture of 3-hydroxyadipic acid, alpha-hydrogenated adipic acid and/or adipic acid comprising:

a process for culturing the genetically modified microorganism according to any one of claims 1 to 3.

Technical Field

The present invention relates to a genetically modified microorganism producing 3-hydroxyadipic acid, α -hydrogenated adipic acid and/or adipic acid in high yield, and a method for producing 3-hydroxyadipic acid, α -hydrogenated adipic acid and/or adipic acid using the genetically modified microorganism.

Background

3-hydroxyadipic acid (IUPAC name: 3-hydroxyhexadiene acid) and α -hydrogenated adipic acid (IUPAC name: (E) -hex-2-enedioic acid) are dicarboxylic acids having 6 carbon atoms. They can be used as raw materials for polymerizing with polyols to form polyesters or with polyamines to form polyamides. Further, compounds obtained by amidation by adding ammonia to their terminals can be used as a raw material for polyamide.

The following documents relating to the production of 3-hydroxyadipic acid or α -hydrogenated adipic acid using a microorganism are known.

Patent document 1 describes a method for producing 1, 3-butadiene using a microorganism having a modified metabolic pathway, and the document describes 3-hydroxyadipate (3-hydroxyadipate) as a metabolic intermediate in a metabolic pathway for biosynthesis of 1, 3-butadiene from acetyl-CoA and succinyl-CoA.

Patent document 2 describes a method for producing adipic acid using a microorganism having a modified metabolic pathway, and describes α -hydrogenated adipic acid (2, 3-dehydroadipate) as a metabolic intermediate in a metabolic pathway for biosynthesis of trans, trans-adipic acid from acetyl-CoA and succinyl-CoA.

Patent documents 3 and 4 describe a method for producing adipic acid and Hexamethylenediamine (HMDA) using an unnatural microorganism, and the documents describe: these substances, although common in the biosynthetic pathway including the reaction of synthesizing 3-oxoadipyl-CoA from acetyl-CoA and succinyl-CoA, are different in the biosynthetic pathway following 3-oxoadipyl-CoA. Patent document 3 describes pyruvate kinase lacking an additional gene for improving formation of HMDA that is a conjugate of growth in production of HMDA, but does not describe a relationship between deficiency in pyruvate kinase and improvement in production of adipic acid.

In addition, the biosynthetic pathways described in patent documents 1 to 4 all describe enzymatic reactions via the reduction of 3-oxoadipyl-CoA to 3-hydroxyadipyl-CoA.

Patent document 5 and patent document 6 disclose methods for producing 3-hydroxyadipic acid and α -hydrogenated adipic acid using a microorganism belonging to the genus serratia, respectively. This document specifically discloses that the production efficiency of 3-hydroxyadipic acid and α -hydrohexadiene diacid can be improved by enhancing the activity of an acyltransferase that catalyzes a reaction from acetyl-CoA and succinyl-CoA to 3-oxoadipyl-CoA, but there is no description about pyruvate kinase.

In addition, patent document 7 discloses a method for improving microorganisms by In silico analysis, which includes: succinic acid productivity is improved by culturing Escherichia coli under anaerobic conditions in the absence of genes pykF, pykA and ptsG encoding pyruvate kinase and phosphotransferase-like enzymes.

Documents of the prior art

Patent document

Patent document 1: japanese Kohyo publication No. 2013-535203

Patent document 2: U.S. patent application publication No. 2011/0124911A1

Patent document 3: japanese patent laid-open publication No. 2015-146810

Patent document 4: japanese Kohyo publication 2011-515111

Patent document 5: international publication No. 2017/209102

Patent document 6: international publication No. 2017/209103

Patent document 7: japanese Kokai publication 2008-527991

Disclosure of Invention

Problems to be solved by the invention

Patent document 1 or2 describes a metabolic pathway by which 3-hydroxyadipic acid or α -hydrogenated adipic acid can be produced in a microorganism, but does not describe that the metabolism is stopped by 3-hydroxyadipic acid or α -hydrogenated adipic acid and the product is secreted into a culture medium. In addition, the following results were not verified: in the case of using a microorganism into which a metabolic pathway has been altered by introducing a nucleic acid encoding an enzyme that catalyzes a reaction of reducing 3-oxoadipyl-CoA described in patent documents 1 to 4 to 3-hydroxyadipyl-CoA, it is actually possible to produce 3-hydroxyadipic acid or α -hydrogenated adipic acid. Patent documents 3 to 6 disclose enhancement of expression of an enzyme gene involved in improvement of the productivity of 3-hydroxyadipic acid, α -hydrohexadiene or adipic acid, but all of the enzyme genes whose expression is enhanced are limited to reactions in the biosynthetic pathway downstream of acetyl-CoA and succinyl-CoA, and enhancement of enzyme activity in metabolic pathways upstream of these reactions is not described.

Accordingly, an object of the present invention is to provide a genetically modified microorganism into which a nucleic acid encoding an enzyme exhibiting excellent activity in a reduction reaction using 3-oxoadipyl-CoA as a substrate is introduced or the expression of which is enhanced, and to produce 3-hydroxyadipic acid, α -hydroadipinic acid and/or adipic acid in high yield by further modifying the upstream metabolic pathway based on the genetically modified microorganism having enhanced enzyme activity, and a method for producing a substance using the genetically modified microorganism.

Means for solving the problems

The present inventors have conducted intensive studies to achieve the above object, and as a result, have found that: the present inventors have completed the present invention by finding that a genetically modified microorganism having an ability to produce 3-hydroxyadipic acid, α -hydrohexadiene diacid and/or adipic acid and impaired pyruvate kinase function and enhanced activity of phosphoenolpyruvate carboxylase kinase and an enzyme catalyzing a reaction of reducing 3-oxoadipyl-CoA to 3-hydroxyadipyl-CoA has an excellent ability to produce 3-hydroxyadipic acid, α -hydrohexadiene diacid and/or adipic acid.

Namely, the present invention provides the following.

(1) A genetically modified microorganism having the ability to produce 3-hydroxyadipate, alpha-hydrohexadiene-adipate and/or adipate and impaired pyruvate kinase function, having enhanced phosphoenolpyruvate carboxylase activity and enzymatic activity catalyzing the reduction of 3-oxoadipyl-CoA to 3-hydroxyadipyl-CoA.

(2) The genetically modified microorganism according to (1), wherein the function of a phosphotransferase enzyme is further impaired.

(3) The genetically modified microorganism according to (1) or (2), wherein the enzyme catalyzing the reaction of reducing the 3-oxoadipyl-CoA to 3-hydroxyadipyl-CoA is any one of the following (a) to (c):

(a) a polypeptide consisting of any one of the amino acid sequences of SEQ ID Nos. 1 to 7;

(b) a polypeptide which comprises an amino acid sequence obtained by substituting, deleting, inserting and/or adding 1 or more amino acids in any one of the amino acid sequences of SEQ ID Nos. 1 to 7 and has an enzymatic activity that catalyzes a reaction in which 3-oxoadipyl-CoA is reduced to 3-hydroxyadipyl-CoA;

(c) a polypeptide having a sequence homology of 70% or more with respect to any one of the amino acid sequences of SEQ ID Nos. 1 to 7, and having an enzymatic activity catalyzing a reaction in which 3-oxoadipyl-CoA is reduced to 3-hydroxyadipyl-CoA.

(4) A process for the manufacture of 3-hydroxyadipic acid, alpha-hydrogenated adipic acid and/or adipic acid comprising: a process for culturing the genetically modified microorganism according to any 1 of (1) to (3).

Effects of the invention

The genetically modified microorganism having the ability to produce 3-hydroxyadipic acid, α -hydrohexadiene diacid and/or adipic acid, and impaired pyruvate kinase function, and enhanced phosphoenolpyruvate carboxylase activity and activity of an enzyme catalyzing the reaction of reducing 3-oxoadipyl-CoA to 3-hydroxyadipyl-CoA, is capable of producing 3-hydroxyadipic acid, α -hydrohexadiene and/or adipic acid in high yield as compared with a microorganism of a parent strain in which the gene has not been altered.

Detailed Description

The invention discovers that: in a microorganism having an ability to produce 3-hydroxyadipic acid, α -hydrogenated adipic acid and/or adipic acid, when the function of pyruvate kinase of the microorganism is impaired and the activity of phosphoenolpyruvate carboxylase and the activity of an enzyme catalyzing the reaction of reducing 3-oxoadipyl-CoA to 3-hydroxyadipyl-CoA are enhanced, 3-hydroxyadipic acid, α -hydrogenated adipic acid and/or adipic acid can be produced at a high yield.

Hereinafter, in the present specification, an enzyme catalyzing a reaction of reducing 3-oxoadipyl-CoA to 3-hydroxyadipyl-CoA may be referred to as "3-oxoadipyl-CoA reductase". In the present specification, phosphoenolpyruvic acid is abbreviated as PEP, 3-hydroxyadipic acid is abbreviated as 3HA, α -hydrohexadiene diacid is abbreviated as HMA, and adipic acid is abbreviated as ADA in some cases.

In the present invention, enhancing the activity of phosphoenolpyruvate carboxylase and the activity of an enzyme catalyzing the reaction of reducing 3-oxoadipyl-CoA to 3-hydroxyadipyl-CoA means the following method: introducing nucleic acids encoding these polypeptides into the host microorganism from the outside of the host microorganism; increasing the copy number of a nucleic acid encoding the polypeptide; a method of modifying a promoter region and a ribosome binding sequence upstream of the polypeptide-encoding region, and the like. These methods may be carried out alone or in combination. The method for introducing the nucleic acid is not particularly limited, and a method of introducing the nucleic acid into a recombinant host microorganism in an expression vector autonomously replicable in the microorganism may be used; or a method of incorporating the nucleic acid into the genome of a microorganism.

The introduced nucleic acid may be 1 kind or plural kinds. And also a combination of the introduction of nucleic acids and the enhancement of expression.

When a nucleic acid encoding a polypeptide expressed in the present invention is incorporated into an expression vector or a host microorganism genome, the nucleic acid for expression vector or genome assembly preferably comprises a promoter, a ribosome binding sequence, a nucleic acid encoding the expressed polypeptide, and a transcription termination sequence. In addition, a gene controlling the promoter activity may be included.

The promoter used in the present invention is not particularly limited as long as it can express the enzyme in the host microorganism, and examples thereof include a gap promoter, a trp promoter, a lac promoter, a tac promoter, and a T7 promoter.

In the present invention, when the nucleic acid is introduced or the expression of the polypeptide is enhanced using an expression vector, the expression vector is not particularly limited as long as it can autonomously replicate in the microorganism, and examples thereof include a pBBR1MCS vector, a pBR322 vector, a pMW vector, a pET vector, a pRSF vector, a pCDF vector, a pACYC vector, and derivatives of the above vectors.

In the present invention, in the case of introducing a nucleic acid or enhancing the expression of a polypeptide using a nucleic acid for genome assembly, the nucleic acid is introduced using site-specific homologous recombination. The method of homologous recombination at a site is not particularly limited, and examples thereof include: methods using lambda Red recombinase and FLP recombinase (Proc NatI Acad Sci USA.2000Jun 6; 97(12): 6640-; a method using lambda Red recombinase and sacB gene (Biosci Biotechnol biochem.2007 Dec; 71(12): 2905-11.).

The method for introducing the nucleic acid for expression vector or genome assembly is not particularly limited as long as the nucleic acid is introduced into the microorganism, and examples thereof include: calcium ion method (Journal of Molecular Biology,53,159 (1970)); electroporation (NM Calvin, PC Hanawalt. J. bacteriol,170(1988), pp.2796-2801), and the like.

Scheme 1 below is an example showing the reaction pathways required for the production of 3-hydroxyadipic acid, alpha-hydrogenated adipic acid and/or adipic acid. Here, reaction A represents a reaction for producing 3-oxoadipyl-CoA and coenzyme A from acetyl-CoA and succinyl-CoA. Reaction B represents the reduction of 3-oxoadipyl-CoA to 3-hydroxyadipyl-CoA. Reaction C represents a reaction to produce 2, 3-dehydroadipoyl-CoA from 3-hydroxyadipoyl-CoA. Reaction D represents the reaction to form adipoyl-CoA from 2, 3-dehydroadipoyl-CoA. Reaction E represents the reaction to produce 3-hydroxyadipic acid from 3-hydroxyadipoyl-CoA. Reaction F represents the reaction to form α -hydrogenated adipic acid from 2, 3-dehydroadipyl-CoA. Reaction G represents the reaction to produce adipic acid from adipoyl-CoA.

[ chemical formula 1]

It is known that in the case of microorganisms having the ability to produce 3-hydroxyadipic acid, α -hydrohexadiene diacid and/or adipic acid, at least the enzyme catalyzing reaction A is present in the biosynthetic pathway shown in scheme 1 above. For reactions to produce 3-hydroxyadipic acid, alpha-hydrohexadiene diacid or adipic acid from 3-oxoadipyl CoA, it is preferred to have the biosynthetic pathway shown in scheme 1. That is, in the case where the genetically modified microorganism of the present invention has an ability to produce 3-hydroxyadipic acid, the host microorganism to be the genetically modified microorganism preferably has an ability to produce 3-oxoadipyl-CoA and coenzyme A from acetyl-CoA and succinyl-CoA (reaction A); the ability to reduce 3-oxoadipyl-CoA to 3-hydroxyadipyl-CoA (reaction B); and the ability to produce 3-hydroxyadipic acid from 3-hydroxyadipoyl-CoA (reaction E). In addition, in the case where the genetically modified microorganism of the present invention has an ability to produce α -hydrogenated adipic acid, the host microorganism to be the genetically modified microorganism preferably has an ability to produce 3-oxoadipyl-CoA and coenzyme a from acetyl-CoA and succinyl-CoA (reaction a); the ability to reduce 3-oxoadipyl-CoA to 3-hydroxyadipyl-CoA (reaction B); the ability to produce 2, 3-dehydroadipoyl-CoA from 3-hydroxyadipoyl-CoA (reaction C); and the ability to produce alpha-hydrogenated hexadiene diacid from 2, 3-dehydroadipyl-CoA (reaction F). Further, in the case where the genetically modified microorganism of the present invention has an ability to produce adipic acid, as a host microorganism of the genetically modified microorganism, it is preferable to have an ability to produce 3-oxoadipyl-CoA and coenzyme A from acetyl-CoA and succinyl-CoA (reaction A); the ability to reduce 3-oxoadipyl-CoA to 3-hydroxyadipyl-CoA (reaction B); the ability to produce 2, 3-dehydroadipoyl-CoA from 3-hydroxyadipoyl-CoA (reaction C); the ability to produce adipyl-CoA from 2, 3-dehydroadipyl-CoA (reaction D); and the ability to produce adipic acid from adipyl-CoA (reaction G).

Genetically modified microorganisms capable of producing 3-hydroxyadipate,. alpha. -hydrogenated adipate and/or adipate in high yield can be obtained as long as the microorganism having these biosynthetic pathways can impair the function of pyruvate kinase to a host microorganism and potentiate the activity of PEP carboxylase kinase and the activity of an enzyme catalyzing the reaction of reducing 3-oxoadipyl-CoA to 3-hydroxyadipyl-CoA.

Examples of the microorganism having an ability to produce 3-hydroxyadipic acid include microorganisms belonging to the following species.

Escherichia coli such as Escherichia fergusonii and Escherichia coli.

Pseudomonas chlororaphis, Pseudomonas putida, Pseudomonas azotoformans, Pseudomonas chlororaphis subsp.

Hafnia alvei et al, Hafnia.

Corynebacterium, such as Corynebacterium acetoacidophilum, Corynebacterium acetoglutamicum, Corynebacterium ammoniagenes and Corynebacterium glutamicum.

Bacillus such as Bacillus badius, Bacillus magasterium and Bacillus roseus.

Streptomyces species such as Streptomyces vinaceae, Streptomyces karnatakensis and Streptomyces olivaceus.

Cupriavidus genus such as Cupriavidus metallidurans, Cupriavidus necator, and Cupriavidus oxalatus.

Acinetobacter species such as Acinetobacter baylyi and Acinetobacter radioresistances.

Alcaligenes faecalis and other Alcaligenes.

Nocardia-like genera such as Nocardioides albus.

Brevibacterium iododinum and the like.

Delftia species of Delftia such as Delftia acidovarans.

Shimwellia blattae et al, Shimwellia.

Aerobacter genus such as Aerobacter cloacae.

Rhizobium radiobacter and the like.

Serratia species such as Serratia grimeii, Serratia ficaria, Serratia fonticola, Serratia odorifera, Serratia plymuthica, Serratia entomophila, and Serratia nematodiphila.

In the case where the genetically modified microorganism of the present invention does not have an ability to produce 3-hydroxyadipic acid by itself, these production abilities may be imparted by appropriately combining nucleic acids encoding enzymes catalyzing reaction A, B, E and introducing the nucleic acids into the microorganism.

Examples of microorganisms which are presumed to have an ability to produce α -hydrogenated adipic acid per se include microorganisms belonging to the following genera.

Escherichia coli such as Escherichia fergusonii and Escherichia coli.

Pseudomonas fluorescens, Pseudomonas putida, Pseudomonas azotoformans, Pseudomonas chlororaphis subsp.

Hafnia alvei et al, Hafnia.

Bacillus such as Bacillus badius.

Cupriavidus genus such as Cupriavidus metallidurans, Cupriavidus numazuensis, Cupriavidus oxalaticus, etc.

Acinetobacter species such as Acinetobacter baylyi and Acinetobacter radioresistances.

Alcaligenes faecalis and other Alcaligenes.

Delftia species of Delftia such as Delftia acidovarans.

Shimwellia blattae et al, Shimwellia.

Serratia species such as Serratia grimeii, Serratia ficaria, Serratia fonticola, Serratia odorifera, Serratia plymuthica, Serratia entomophila, and Serratia nematodiphila.

In the case where the genetically modified microorganism of the present invention does not have an ability to produce α -hydrogenated adipic acid by itself, these production abilities may be imparted by appropriately combining nucleic acids encoding enzymes catalyzing reaction A, B, C, F and introducing them into the microorganism.

Examples of microorganisms presumed to have an ability to produce adipic acid per se include microorganisms belonging to the genus thermophilus such as Thermobifida fusca. In the case where the genetically modified microorganism of the present invention does not have an ability to produce adipic acid by itself, these production abilities can be imparted by appropriately combining nucleic acids encoding enzymes catalyzing reaction A, B, C, D, G and introducing them into the microorganism.

The microorganism which can be used as a host for obtaining a genetically modified microorganism in the present invention is preferably the above-listed microorganisms, and particularly preferably microorganisms belonging to the genera Escherichia (Escherichia), Serratia (Serratia), Hafnia (Hafnia), Pseudomonas (Psuedomonas), Corynebacterium (Corynebacterium), Bacillus (Bacillus), Streptomyces (Streptomyces), Cupriavidus (Cupriavidus), Acinetobacter (Acinetobacter), Alcaligenes (Alcaligenes), Brevibacterium (Brevibacterium), Delftia (Delftia), Shimwell, Aerobacter (Aerobacter), Rhizobium (Rhizobium), Thermomyces (Thermobifida), Clostridium (Clostridium), Schizosaccharomyces (Schizosaccharomyces), Kluyveromyces (Kluyveromyces), Pichia (Pichia), and Candida (Candida). Among these, microorganisms belonging to the genera Escherichia, Serratia, Hafnia and Pseudomonas are particularly preferable.

Specific examples of the enzyme catalyzing the reaction of reducing the 3-oxoadipoyl-CoA to 3-hydroxyadipoyl-CoA include the polypeptides described in the following (a) to (c).

(a) A polypeptide comprising the amino acid sequence of any one of SEQ ID Nos. 1 to 7;

(b) a polypeptide which comprises an amino acid sequence obtained by substituting, deleting, inserting and/or adding 1 or more amino acids in the amino acid sequence of any one of SEQ ID Nos. 1 to 7 and has an enzymatic activity that catalyzes a reaction in which 3-oxoadipyl-CoA is reduced to 3-hydroxyadipyl-CoA;

(c) a polypeptide having a sequence homology of 70% or more with respect to the amino acid sequence of any one of SEQ ID Nos. 1 to 7, and having an activity of reducing 3-oxoadipyl-CoA to 3-hydroxyadipyl-CoA.

In addition, an enzyme classified under EC1.1.1.35 as a 3-hydroxyacyl-CoA dehydrogenase and an enzyme classified under EC1.1.1.157 as a 3-hydroxybutyryl-CoA dehydrogenase may also be used as the enzymes having 3-oxoadipyl-CoA reductase activity. Specifically, as enzymes catalyzing the reaction of reducing 3-oxoadipyl-CoA to 3-hydroxyadipyl-CoA, PaaH (NCBI-Protein ID: NP 745425.1) derived from Pseudomonas putida KT2440 strain, PaaH (NCBI-Protein ID: NP 415913.1) derived from Escherichia coli strain K-12substr.MG1655 strain, DcaH (NCBI-Protein ID: CAG68533.1) derived from Acinetobacter baylyi ADP1 strain, PaaH (NCBI-Protein ID: WP 063197120) derived from Serratia plymuthica NBRC102599 strain, and polypeptide (NCBI-Protein ID: WP 033633399.1) derived from Serratia nematodiphila DSM21420 strain can be mentioned. Among these, the polypeptides described in (a) to (c) above are preferred.

In the present invention, the "1 or more" range is preferably 10 or less, more preferably 5 or less, particularly preferably 4 or less, and most preferably 1 or 2or less, for a polypeptide having an enzymatic activity of 3-oxoadipyl-CoA reductase, which polypeptide is composed of an amino acid sequence in which 1 or more amino acids are substituted, deleted, inserted, and/or added in an amino acid sequence described in any one of SEQ ID Nos. 1 to 7. Here, when an amino acid is substituted, the possibility that the activity of the polypeptide is maintained is increased when the amino acid is substituted with a qualitatively similar amino acid (so-called conservative substitution). That is, in the case of substitution of an amino acid, even if the substitution is made with an amino acid having similar properties, physiological activity is often maintained, and therefore, the substitution with an amino acid having similar properties is preferable. That is, the 20 amino acids constituting the natural protein can be classified into the following groups having similar properties: neutral amino acids having low polar side chains (Gly, Ile, Val, Leu, Ala, Met, Pro); neutral amino acids having a hydrophilic side chain (Asn, Gln, Thr, Ser, Tyr, Cys); acidic amino acids (Asp, Glu); basic amino acids (Arg, Lys, His); aromatic amino acids (Phe, Tyr, Trp) are not changed in properties in many cases if substitution is made between them.

In the present invention, the preferred range of sequence homology for the polypeptide having a sequence homology of 70% or more with respect to any one of the amino acid sequences of SEQ ID Nos. 1 to 7 and having the enzymatic activity of 3-oxoadipyl-CoA reductase to be used is preferably 80% or more, more preferably 85% or more, still more preferably 90% or more, still more preferably 95% or more, still more preferably 97% or more, and still more preferably 99% or more.

In the present invention, "sequence homology" refers to the proportion (percentage) of identical amino acids or bases with respect to all amino acid sequences (including amino acids as a translation initiation point) or base sequences (including a start codon) of an overlap (overlap) in an optimal alignment (alignment) in the case where 2 amino acid sequences or base sequences are arranged with or without introduction of a Gap (Gap). In the formula (1), the relatively short one has a sequence length of 400 amino acids or more, and when less than 400 amino acids, it is not defined as sequence homology. Sequence homology can be readily studied using the algorithm common in the art, BLAST (basic Local Alignment Search tool). For example, with respect to BLAST, one can readily study sequence homology using default parameters and available from the website of NCBI (national Center for Biotechnology information) or KEGG (Kyoto Encyclopedia of Genes and genomes). In addition, sequence homology can also be studied using the same function carried by software such as Genetyx.

Sequence homology (%). number of matches (intervals not counted) per shorter length of sequence (length excluding both ends interval). times.100. equation (1).

According to the formula (1), when the sequence homology among the amino acid sequences described in the sequence numbers 1 to 7 is calculated using the function (% Identity Matrix) of Genetyx, the value between the sequence numbers 2 and 4 having the lowest value of sequence homology is 71.51%, and the amino acid sequences described in the sequence numbers 1 to 7 have at least 70% or more of sequence homology with each other. The results of sequence homology calculated using Genetyx are shown in Table 1. In table 1 below, the leftmost digit represents a serial number.

[ Table 1]

Each of the amino acid sequences shown in sequence nos. 1 to 7 was used as Query, and sequence homology with all amino acid sequences registered in the amino acid database (Non-redundant protein sequences) of NCBI was investigated using BLASTP, and as a result, sequences having sequence homology of 70% or more were derived from a bacterium belonging to the genus Serratia (Serratia).

The polypeptides described in sequence numbers 1 to 7 of (a) above all have a common sequence 1 composed of 24 amino acid residues shown by sequence number 173 among 15 to 38 amino acid residues from the N-terminal side (hereinafter, for convenience, the first amino acid residue from the N-terminal side is referred to as "a.a.". accordingly, for example, the 15 to 38 amino acid residues from the N-terminal side are referred to simply as "15 to 38 a.a."). In common sequence 1 Xaa is any amino acid residue, 13a.a. is preferably phenylalanine or leucine, 15a.a. is preferably leucine or glutamine, 16a.a. is preferably lysine or asparagine, 17a.a. is glycine or serine, more preferably glycine, 19a.a. is preferably proline or arginine, 21a.a. is preferably leucine, methionine or valine. Common sequence 1 corresponds to the NAD + binding residues and the surrounding amino acid residues. Such as Biochimie 2012 Feb; 94(2):471-8. the NAD + binding residues are characterized by: the 24 th amino acid residue of common sequence 1 is aspartic acid, but in common sequence 1 is asparagine. It is believed that: the polypeptides described in SEQ ID Nos. 1 to 7 have the common sequence 1, and exhibit excellent enzymatic activity as 3-oxoadipyl-CoA reductase.

The polypeptides of (b) and (c) preferably have a common sequence 1 consisting of 24 amino acid residues represented by the sequence No. 173 within 1 to 200 a.a.. The position of the common sequence is more preferably within 1 to 150a.a., and still more preferably within 1 to 100 a.a.. Specific examples thereof include amino acid sequences of SEQ ID Nos. 8 to 86. The amino acid sequences of SEQ ID Nos. 8 to 86 have a common sequence 1 consisting of 24 amino acid residues shown in SEQ ID No. 173 in 15 to 38 a.a.. The sequence homology between the amino acid sequences of SEQ ID Nos. 8 to 86 and any one of the amino acid sequences of SEQ ID Nos. 1 to 7 is 90% or more. The results of sequence homology calculated using Genetyx are shown in tables 2-1 to 2-3 and tables 3-1 to 3-3.

[ Table 2-1]

[ tables 2-2]

[ tables 2 to 3]

[ Table 3-1]

[ tables 3-2]

[ tables 3 to 3]

The nucleic acid encoding the polypeptide described in (a) to (C) of the present invention may comprise a sequence obtained by further adding a peptide or protein to the N-terminus and/or C-terminus of the polypeptide. Examples of such a peptide or protein include a secretion signal sequence, a transport protein, a binding protein, a labeling peptide for purification, a fluorescent protein, and the like. For such a peptide or protein, those skilled in the art can select a peptide or protein having an added function according to the purpose and add it to the polypeptide of the present invention. It is to be noted that such a peptide or protein is not included in the sequence homology of the amino acid sequence.

The nucleic acid encoding the polypeptide of SEQ ID Nos. 1 to 86 is not particularly limited as long as it is a nucleotide sequence that can be translated into the amino acid sequence of SEQ ID Nos. 1 to 86, and can be determined by referring to codons (standard genetic code) corresponding to the respective amino acids. In this case, the nucleotide sequence may be redesigned using codons commonly used in the host microorganism used in the present invention.

Specific examples of the nucleotide sequences of nucleic acids encoding polypeptides having amino acid sequences of SEQ ID Nos. 1 to 86 include the nucleotide sequences of SEQ ID Nos. 87 to 172.

In the present invention, whether or not a polypeptide encoded by a nucleic acid has 3-oxoadipyl-CoA reductase activity is judged by: the following transformants A and B were prepared, and the results of the culture test were confirmed, and if 3-hydroxyadipic acid or α -hydrohexadiene diacid was produced in the culture broth, the nucleic acid encodes a polypeptide having 3-oxoadipyl-CoA reductase activity. The determination method is described using the biosynthesis pathway shown in scheme 1 above.

The transformant A has enzymes catalyzing the reactions A, E and F. The transformant B has enzymes catalyzing the reaction A, the reaction C, the reaction E and the reaction F.

First, a transformant a was prepared. Plasmids were prepared which were capable of expressing enzymes catalyzing reaction A, reaction E and reaction F, respectively. Reactions E and F may be catalyzed by the same enzyme. This plasmid was introduced into a strain of a microorganism having no ability to produce any one of 3-hydroxyadipic acid, α -hydrogenated adipic acid, and adipic acid, i.e., Escherichia coli BL21(DE 3). The transformant A is obtained by introducing an expression plasmid into the obtained transformant, wherein the expression plasmid is obtained by recombining a nucleic acid encoding a polypeptide to be investigated for the presence or absence of enzymatic activity, downstream of an appropriate promoter. The transformant A was cultured, and it was confirmed whether or not 3-hydroxyadipic acid was contained in the culture solution after the culture. When 3-hydroxyadipic acid was confirmed in the culture medium, transformant B was subsequently prepared. The transformant B is obtained by preparing a plasmid capable of expressing an enzyme catalyzing the reaction C into the transformant A and introducing the plasmid. The transformant B was cultured to confirm whether or not the culture medium after the culture contained α -hydrogenated adipic acid. If it is confirmed that α -hydrogenated adipic acid is contained in the culture medium after the culture, it is found that 3-hydroxyadipic acid produced by transformant A and α -hydrogenated adipic acid produced by transformant B are produced via 3-hydroxyadipyl-CoA, and thus the target polypeptide is judged to have 3-oxoadipyl-CoA reductase activity.

As the Gene encoding an enzyme catalyzing reaction A, pcaF (NCBI Gene ID: 1041755, SEQ ID NO: 174) derived from Pseudomonas putida KT2440 strain can be used.

As genes encoding enzymes catalyzing reactions E and F, the full-length contiguous sequences including pcaI and pcaJ (NCBI Gene ID: 1046613, 1046612, SEQ ID Nos. 175, 176) from Pseudomonas putida KT2440 strain can be used. The polypeptides encoded by pcaI and pcaJ catalyze reactions E and F by forming a complex.

As the nucleic acid encoding the enzyme catalyzing reaction C, the Pseudomonas putida KT2440 strain Gene paaF (NCBI Gene ID: 1046932, SEQ ID NO: 177) can be used.

The transformant A and the transformant B were cultured in the following manner. In addition, an antibiotic for stably maintaining the plasmid and a substance for inducing expression of the polypeptide encoded by the recombinant nucleic acid may be added appropriately during the culture. The one platinum loop transformant A or B was inoculated into 5mL of a medium I (10g/L Bacto-tryptone, Difco Laboratories, Inc.), 5g/L yeast extract for bacteria (Difco Laboratories, Inc.), 5g/L sodium chloride) adjusted to pH7, and shake-cultured at 30 ℃ for 18 hours for 120min-1 to obtain a preculture solution. Then, 0.25mL of this preculture solution was added to 5mL of medium II (10g/L of succinic acid, 10g/L of glucose, 1g/L of ammonium sulfate, 50mM of potassium phosphate, 0.025g/L of magnesium sulfate, 0.0625mg/L of iron sulfate, 2.7mg/L of manganese sulfate, 0.33mg/L of calcium chloride, 1.25g/L of sodium chloride, 2.5g/L of bacto-tryptone, 1.25g/L of bacto-yeast extract) adjusted to pH6.5, and the mixture was subjected to shake culture at 30 ℃ for 24 hours at 120 min-1. It was confirmed whether the obtained culture broth contained 3-hydroxyadipic acid or α -hydrogenated adipic acid.

The culture broth was centrifuged, and the supernatant was analyzed by LC-MS/MS to confirm that the culture broth contained 3-hydroxyadipic acid or α -hydrohexadiene diacid. The conditions for the analysis are as follows.

HPLC: 1290Infinity (Agilent Technologies, Inc.)

Column: synergi hydro-RP (manufactured by Phenomenex Co., Ltd.), length of 100mm, inner diameter of 3mm, and particle diameter of 2.5 μm

Mobile phase: 0.1% aqueous formic acid/methanol 70/30

Flow rate: 0.3 mL/min

Column temperature: 40 deg.C

An LC detector: DAD (210nm)

MS/MS: Triple-Quad LC/MS (manufactured by Agilent Technologies Co., Ltd.)

An ionization method: ESI negative mode.

The activity value of 3-oxoadipyl-CoA reductase was calculated by measuring 3-hydroxyadipyl-CoA produced by using purified 3-oxoadipyl-CoA reductase using 3-oxoadipyl-CoA prepared by carrying out an enzymatic reaction from 3-oxoadipate as a substrate. The specific method is as follows.

3-oxoadipic acid can be prepared by a known method (for example, the method described in reference example 1 of WO 2017/099209).

Preparation of 3-oxoadipyl-CoA solution: the full length of the nucleic acid encoding the CoA transferase (pcaI and pcaJ, NCBI-Gene ID: 1046613 and 1046612) was amplified by PCR using genomic DNA of Pseudomonas putida KT2440 strain as a template according to a conventional method. The base sequences of the primers used in this PCR are, for example, SEQ ID Nos. 194 and 195. This amplified fragment was inserted into the KpnI site of pRSF-1b (manufactured by Novagen) as an expression vector for Escherichia coli, and was made into a frame (frame) identical to the histidine tag sequence. This plasmid was introduced into Escherichia coli BL21(DE3), and the expression of the enzyme was induced by using isopropyl-. beta. -thiogalactopyranoside (IPTG) according to a conventional method, followed by purification using a histidine tag derived from the culture broth to obtain a CoA transferase solution. This solution was used to prepare an enzyme reaction solution for 3-oxoadipyl-CoA preparation having the following composition, and after reacting at 25 ℃ for 3 minutes, the enzyme was removed by treating with a UF membrane (Amicon Ultra-0.5mL10K, manufactured by Merck Millipore Co., Ltd.), and the obtained permeate was used as a 3-oxoadipyl-CoA solution.

(enzyme reaction solution)

100mM Tris-HCl(pH8.2)

10mM MgCl₂

0.5mM succinyl-CoA

5mM 3-oxoadipic acid sodium salt

2 μm CoA transferase

Confirmation of 3-oxoadipyl-CoA reductase Activity: the full length of the nucleic acid encoding 3-oxoadipyl-CoA reductase was amplified by PCR using the genomic DNA of the subject microorganism strain as a template according to a conventional method. The base sequences of the primers used in this PCR are, for example, SEQ ID Nos. 196 and 197. This amplified fragment was inserted into the BamHI site of an expression vector for Escherichia coli, pACYCDuet-1 (manufactured by Novagen) to form a framework identical to the histidine tag sequence. This plasmid was introduced into Escherichia coli BL21(DE3), and the expression of the enzyme was induced by isopropyl-. beta. -thiogalactopyranoside (IPTG) according to a conventional method, followed by purification using a histidine tag derived from the culture broth to obtain a 3-oxoadipyl-CoA reductase solution. 3-Oxadiapyl-CoA reductase activity can be confirmed by preparing an enzyme reaction solution having the following composition using the enzyme solution and measuring the amount of 3-hydroxyadipyl-CoA produced at 25 ℃.

(enzyme reaction solution)

100mM Tris-HCl(pH8.2)

10mM MgCl₂

150 μ L/mL 3-oxoadipyl-CoA solution

0.5mM NADH

1mM dithiothreitol

10 μm 3-oxoadipyl-CoA reductase

Next, specific examples of enzymes catalyzing reactions A, C to G are shown. As an enzyme catalyzing reaction A for producing 3-oxoadipyl-CoA, for example, acyltransferase (. beta. -ketothiolase) and the like can be used. The acylase is not particularly limited in classification based on EC number, but preferably an acylase classified into EC 2.3.1-, and specifically, there may be mentioned: an enzyme classified under EC2.3.1.174 as a 3-oxoadipyl-CoA thiolase, an enzyme classified under EC2.3.1.9 as an acetyl CoA C-acetyltransferase, and an enzyme classified under EC2.3.1.16 as an acetyl CoA C-acetyltransferase. Of these, PaaJ (NCBI-Protein ID: NP 415915) derived from Escherichia coli MG1655 strain, PcaF (NCBI-Protein ID: NP 743536) derived from Pseudomonas putida KT2440 strain, and the like are preferably used.

Whether the above acyltransferase can produce 3-oxoadipyl-CoA with succinyl-CoA and acetyl-CoA as substrates is confirmed by the following method: combining the reaction of producing 3-oxoadipyl-CoA by purification of acyltransferase and the reduction reaction of 3-oxoadipyl-CoA reductase by purification using 3-oxoadipyl-CoA as substrate, the amount of NADH reduction accompanying the reduction of 3-oxoadipyl-CoA is measured. Specific measurement methods are described below, for example.

Confirmation of acyltransferase Activity: the entire length of the nucleic acid encoding the acylase was amplified by PCR using the genomic DNA of the subject microorganism strain as a template according to a conventional method. This amplified fragment was inserted into the SacI site of an expression vector pACYCDuet-1 for Escherichia coli (manufactured by Novagen) so as to form a framework identical to the histidine tag sequence. This plasmid was introduced into Escherichia coli BL21(DE3), and the expression of the enzyme was induced by using isopropyl-. beta. -thiogalactopyranoside (IPTG) according to a conventional method, followed by purification using a histidine tag derived from the culture broth to obtain an acyltransferase solution. The acyltransferase activity can be confirmed by: using this enzyme solution, an enzyme reaction solution having the following composition was prepared, and the decrease in absorption at 340nm accompanying the oxidation of NADH at 30 ℃ was measured.

100mM Tris-HCl(pH8.0)

10mM MgCl₂

0.1mM succinyl-CoA

0.2mM acetyl-CoA

0.2mM NADH

1mM dithiothreitol

10 μ g/mL 3-oxoadipyl-CoA reductase

5 μ g/mL acyltransferase

Whether or not the enzyme possessed by the microorganism has an acyltransferase activity is confirmed by performing the above-described measurement using CFE instead of purified acyltransferase. Specific measurement methods for coliform bacteria are described below, for example.

Preparation of CFE: escherichia coli MG1655 strain as an object of activity measurement of one platinum loop was inoculated into 5mL of a medium (medium composition: tryptone at 10g/L, yeast extract at 5g/L, sodium chloride at 5 g/L) adjusted to pH7, and shake-cultured at 30 ℃ for 18 hours. The resulting culture broth was added to 5mL of a medium (medium composition: 10g/L of tryptone, 5g/L of yeast extract, 5g/L of sodium chloride, 2.5mM of ferulic acid, 2.5mM of p-coumaric acid, 2.5mM of benzoic acid, 2.5mM of cis, cis-hexadiene diacid, 2.5mM of protocatechuic acid, 2.5mM of catechol, 2.5mM of 3OA, 2.5mM of 3-hydroxyadipic acid, 2.5mM of α -hydrohexadiene diacid, 2.5mM of adipic acid, 2.5mM of phenylethylamine) adjusted to pH7, and shake-cultured at 30 ℃ for 3 hours.

To the obtained culture solution, 10mL of 0.9% sodium chloride was added, and the cells were centrifuged to remove the supernatant, and the operation was repeated 3 times to wash the cells. The washed cells were suspended in 1mL of Tris-HCl buffer composed of 100mM Tris-HCl (pH8.0) and 1mM dithiothreitol, and glass beads (. phi.0.1 mM) were added to the resulting suspension to disrupt the cells at 4 ℃ using an ultrasonic disrupter. The cell disruption solution was centrifuged, 0.5mL of the supernatant obtained was passed through a UF membrane (Amicon Ultra-0.5mL10K, Merck Millipore Co., Ltd.), the permeate was removed, 0.4mL of Tris-HCl buffer was added, the process was repeated 3 times to remove low molecular weight impurities, and the solution was suspended again in Tris-HCl buffer to a liquid volume of 0.1mL and used as CFE. The CFE was added in an amount of 0.05mL to the total amount of 0.1mL of the enzyme reaction solution in place of the purified enzyme, and the enzyme activity was confirmed.

As an enzyme catalyzing reaction C which produces 2, 3-dehydroadipoyl-CoA, for example, enoyl-CoA hydratase or the like can be used. The enoyl-CoA hydratase is not particularly limited in the classification based on the EC number, but is preferably an enoyl-CoA hydratase classified into EC number 4.2.1, and specifically, an enzyme classified into EC4.2.1.17 as enoyl-CoA hydratase or2, 3-dehydroadipyl-CoA hydratase may be mentioned. Of these, PaaF derived from Escherichia coli MG1655 strain (NCBI-Protein ID: NP 415911), PaaF derived from Pseudomonas putida KT2440 strain (NCBI-Protein ID: NP 745427), and the like are preferably used.

Since the reaction catalyzed by enoyl-CoA hydratase is generally a reversible reaction, the activity of enoyl-CoA hydratase catalyzing the reaction producing 2, 3-dehydroadipoyl-CoA using 3-hydroxyadipoyl-CoA as a substrate was confirmed by the following method: 3-hydroxyadipyl-CoA produced using a purified enoyl-CoA hydratase was assayed using 2, 3-dehydroadipyl-CoA prepared from α -hydrogenated adipic acid by an enzymatic reaction as a substrate. Specific measurement methods are described below, for example.

Preparation of α -hydrogenated adipic acid: alpha-hydrogenated adipic acid can be prepared by the method described in reference example 1 of WO2016/199858A 1.

Preparation of 2, 3-dehydroadipoyl-CoA solution: the full length of the nucleic acid encoding the CoA transferase (pcaI and pcaJ, NCBI-Gene ID: 1046613 and 1046612) was amplified by PCR using genomic DNA of Pseudomonas putida KT2440 strain as a template according to a conventional method. This amplified fragment was inserted into the KpnI site of pRSF-1b (manufactured by Novagen) as an expression vector for Escherichia coli, and was framed in the same manner as the histidine tag sequence. This plasmid was introduced into Escherichia coli BL21(DE3), and the expression of the enzyme was induced by using isopropyl-. beta. -thiogalactopyranoside (IPTG) according to a conventional method, followed by purification using a histidine tag derived from the culture broth to obtain a CoA transferase solution. This solution was used to prepare an enzyme reaction solution for 2, 3-dehydroadipoyl-CoA preparation having the following composition, and after a reaction at 30 ℃ for 10 minutes, the enzyme was removed by treatment with a UF membrane (Amicon Ultra-0.5mL10K, manufactured by Merck Millipore Co., Ltd.), and the obtained permeate was used as a2, 3-dehydroadipoyl-CoA solution.

Enzyme reaction solution for 2, 3-dehydroadipoyl-CoA production

100mM Tris-HCl(pH8.0)

10mM MgCl₂

0.4mM succinyl-CoA

2mM alpha-hydrogenated adipic acid sodium salt

20 μ g/mL CoA transferase

Confirmation of enoyl-CoA hydratase activity: the full length of the nucleic acid encoding enoyl-CoA hydratase was amplified by PCR using the genomic DNA of the subject microorganism strain as a template according to a conventional method. This amplified fragment was inserted into the NdeI site of an expression vector pET-16b for Escherichia coli (manufactured by Novagen) so as to form a frame identical to the histidine tag sequence. This plasmid was introduced into Escherichia coli BL21(DE3), and the expression of the enzyme was induced by isopropyl-. beta. -thiogalactopyranoside (IPTG) according to a conventional method, followed by purification using a histidine tag derived from the culture broth to obtain an enoyl-CoA hydratase solution. enoyl-CoA hydratase activity was confirmed by the following method: an enzyme reaction solution having the following composition was prepared using this solution, after a reaction at 30 ℃ for 10 minutes, treated with a UF membrane (Amicon Ultra-0.5mL10K, manufactured by Merck Millipore Co., Ltd.) to remove the enzyme, and the resulting permeate was examined for 3-hydroxyadipoyl-CoA by high performance liquid chromatography-mass spectrometry (LC-MS/MS) (Agilent Co., Ltd.).

100mM Tris-HCl(pH8.0)

10mM MgCl₂

300 μ L/mL 2, 3-dehydroadipyl-CoA solution

1mM dithiothreitol

20 μ g/mL enoyl-CoA hydratase

Whether or not the enzyme itself expressed in the host microorganism used in the present invention has enoyl-CoA hydratase activity is confirmed by adding 0.05mL of CFE instead of purified enoyl-CoA hydratase to 0.1mL of the enzyme reaction solution in total and performing the above measurement. Specific examples of the method for producing CFE to be used for Escherichia coli include the method for confirming the acyltransferase activity.

As an enzyme catalyzing adipoyl-CoA producing reaction D, for example, enoyl-CoA reductase or the like can be used. As the enoyl-CoA reductase, there is no particular limitation in the classification based on the EC number, but enoyl-CoA reductases classified into EC number 1.3-are preferable, and specifically, an enzyme classified into EC1.3.1.44 as trans-2-enoyl-CoA reductase and an enzyme classified into EC1.3.8.7 as acyl-CoA dehydrogenase are included. Specific examples of these are shown in, for example, Japanese Kokai publication 2011-; 119(4):1057-63, etc., among these, TER (UniProt KB: Q5EU90) derived from Euglena gracilis Z strain, Ttu 1647(NCBI-Protein ID: AAZ55682) derived from Thermobifida fusca YX strain, DcaA (NCBI-Protein ID: AAL09094.1) derived from Acinetobacter baylyi ADP1 strain, etc. are preferably used.

The activity of enoyl-CoA reductase to produce adipyl-CoA using 2, 3-dehydroadipyl-CoA as a substrate was confirmed by the following method: the amount of NADH reduction accompanying the reduction of 2, 3-dehydroadipoyl-CoA was measured using a purified enoyl-CoA reductase with 2, 3-dehydroadipoyl-CoA prepared from α -hydrogenated adipic acid by an enzymatic reaction as a substrate.

The preparation of α -hydrogenated adipic acid and the preparation of a2, 3-dehydroadipoyl-CoA solution can be performed in the same manner as described above.

Confirmation of enoyl-CoA reductase activity: the full length of the nucleic acid encoding enoyl-CoA reductase is amplified by PCR using the genomic DNA of the subject microorganism strain as a template according to a conventional method. This amplified fragment was inserted into the NdeI site of an expression vector pET-16b for Escherichia coli (manufactured by Novagen) so as to form a frame identical to the histidine tag sequence. This plasmid was introduced into Escherichia coli BL21(DE3), and the expression of the enzyme was induced by isopropyl-. beta. -thiogalactopyranoside (IPTG) according to a conventional method, followed by purification using a histidine tag derived from the culture broth to obtain an enoyl-CoA reductase solution. enoyl-CoA reductase activity can be confirmed by the following method: using this enzyme solution, an enzyme reaction solution having the following composition was prepared, and the decrease in absorption at 340nm accompanying the oxidation of NADH at 30 ℃ was measured.

100mM Tris-HCl(pH8.0)

10mM MgCl₂

300 μ L/mL 2, 3-dehydroadipyl-CoA solution 0.2mM NADH

1mM dithiothreitol

20 μ g/mL enoyl-CoA reductase

Whether or not the enzyme itself expressed in the host microorganism used in the present invention has enoyl-CoA reductase activity can be confirmed by adding 0.05mL of CFE instead of purified enoyl-CoA reductase to 0.1mL of the total amount of the enzyme reaction solution and performing the above measurement. Specific examples of the method for producing CFE to be used for Escherichia coli include the method for confirming the acyltransferase activity.

As the enzyme catalyzing reaction E for producing 3-hydroxyadipic acid, reaction F for producing α -hydrohexadiene diacid, and reaction G for producing adipic acid, CoA transferase, acyl-CoA hydratase or the like can be used, but CoA transferase is preferred.

The CoA transferase is not particularly limited in the classification based on the EC number, but is preferably a CoA transferase classified into EC number 2.8.3-, and specifically, examples thereof include CoA transferase or acyl-CoA transferase classified into EC 2.8.3.6.

In the present invention, "CoA transferase" refers to an enzyme having a catalytic activity (CoA transferase activity) of a reaction of producing a carboxylic acid and succinyl-CoA using acyl-CoA and succinic acid as substrates.

Among the enzymes catalyzing reaction E for producing 3-hydroxyadipic acid and reaction F for producing α -hydrohexadiene diacid, PcaI and PcaJ (NCBI-Protein ID: NP 746081 and NP 746082) derived from Pseudomonas putida KT2440 strain and the like are preferably used.

In addition, among enzymes catalyzing reaction G for producing adipic acid, DcaI and DcaJ (NCBI-Protein ID: CAG68538 and CAG68539) derived from Acinetobacter baylyi ADP1 strain, and the like are preferably used.

Since this enzymatic reaction is a reversible reaction, the CoA transferase activity using 3-hydroxyadipyl-CoA, 2, 3-dehydroadipyl-CoA or adipyl-CoA as a substrate can be confirmed by the following method: 3-hydroxyadipyl-CoA, 2, 3-dehydroadipyl-CoA or adipyl-CoA generated using a purified CoA transferase was assayed using 3-hydroxyadipic acid and succinyl-CoA, α -hydrogenated adipyl-diacid and succinyl-CoA, or adipic acid and succinyl-CoA as a substrate. Specific measurement methods are described below, for example.

Preparation of 3-hydroxyadipic acid: 3-Hydroxyadipic acid can be prepared by the method described in reference example 1 of WO2016/199856A 1.

Confirmation of CoA transferase Activity with 3-hydroxyadipic acid as substrate: according to a conventional method, PCR is performed using genomic DNA of the subject microorganism strain as a template, and the entire length of the nucleic acid encoding CoA transferase is amplified. This amplified fragment was inserted into the KpnI site of an expression vector pRSF-1b for Escherichia coli (manufactured by Novagen) so as to have the same frame as the histidine tag sequence. This plasmid was introduced into Escherichia coli BL21(DE3), and the expression of the enzyme was induced by using isopropyl-. beta. -thiogalactopyranoside (IPTG) according to a conventional method, followed by purification using a histidine tag derived from the culture broth to obtain a CoA transferase solution. The CoA transferase activity was confirmed by the following method: this solution was used to prepare an enzyme reaction solution having the following composition, after a reaction at 30 ℃ for 10 minutes, the enzyme was removed by treatment with a UF membrane (Amicon Ultra-0.5mL10K, manufactured by Merck Millipore Co., Ltd.), and 3-hydroxyadipyl-CoA in the permeate was detected by high performance liquid chromatography-mass spectrometry (LC-MS/MS) (Agilent Co., Ltd.).

100mM Tris-HCl(pH8.0)

10mM MgCl₂

0.4mM succinyl-CoA

2mM 3-Hydroxyadipic acid sodium salt

20 μ g/mL CoA transferase

Preparation of α -hydrogenated adipic acid: alpha-hydrogenated adipic acid can be prepared by the method described in reference example 1 of WO2016/199858A 1.

Confirmation of CoA transferase Activity with α -Hydrohexadienoic acid as substrate: according to a conventional method, PCR is performed using genomic DNA of the subject microorganism strain as a template, and the entire length of the nucleic acid encoding CoA transferase is amplified. This amplified fragment was inserted into the KpnI site of an expression vector pRSF-1b for Escherichia coli (manufactured by Novagen) so as to have the same frame as the histidine tag sequence. This plasmid was introduced into Escherichia coli BL21(DE3), and the expression of the enzyme was induced by using isopropyl-. beta. -thiogalactopyranoside (IPTG) according to a conventional method, followed by purification using a histidine tag derived from the culture broth to obtain a CoA transferase solution. The CoA transferase activity was confirmed by the following method: this solution was used to prepare an enzyme reaction solution having the following composition, after a reaction at 30 ℃ for 10 minutes, treated with a UF membrane (Amicon Ultra-0.5mL10K, manufactured by Merck Millipore Co., Ltd.) to remove the enzyme, and the permeate was examined for 2, 3-dehydroadipoyl-CoA by high performance liquid chromatography-mass spectrometry (LC-MS/MS) (Agilent Co., Ltd.).

100mM Tris-HCl(pH8.0)

10mM MgCl₂

0.4mM succinyl-CoA

2mM alpha-hydrogenated adipic acid sodium salt

20 μ g/mL CoA transferase

Confirmation of CoA transferase Activity with adipic acid as matrix: according to a conventional method, PCR is performed using genomic DNA of the subject microorganism strain as a template, and the entire length of the nucleic acid encoding CoA transferase is amplified. This amplified fragment was inserted into the KpnI site of an expression vector pRSF-1b for Escherichia coli (manufactured by Novagen) so as to have the same frame as the histidine tag sequence. This plasmid was introduced into Escherichia coli BL21(DE3), and the expression of the enzyme was induced by using isopropyl-. beta. -thiogalactopyranoside (IPTG) according to a conventional method, followed by purification using a histidine tag derived from the culture broth to obtain a CoA transferase solution. The CoA transferase activity was confirmed by the following method: this solution was used to prepare an enzyme reaction solution having the following composition, after a reaction at 30 ℃ for 10 minutes, treated with a UF membrane (Amicon Ultra-0.5mL10K, manufactured by Merck Millipore Co., Ltd.) to remove the enzyme, and the permeated solution was examined for adipoyl-CoA by high performance liquid chromatography-mass spectrometry (LC-MS/MS) (Agilent Co., Ltd.).

100mM Tris-HCl(pH8.0)

10mM MgCl₂

0.4mM succinyl-CoA

2mM adipic acid sodium salt

20 μ g/mL CoA transferase

Whether or not the enzyme itself expressed in the host microorganism used in the present invention has the CoA transferase activity is confirmed by adding 0.05mL of CFE instead of purified CoA transferase to 0.1mL of the enzyme reaction solution in total and performing the above measurement. Specific examples of the method for producing CFE to be used for Escherichia coli include the method for confirming the acyltransferase activity.

In the present invention, when a nucleic acid encoding any one of PEP carboxylase, acyltransferase, 3-oxoadipyl-CoA reductase, enoyl-CoA hydratase, enoyl-CoA reductase and CoA transferase is introduced into a host microorganism, the nucleic acid may be artificially synthesized based on the amino acid sequence of the enzyme present in the database or may be isolated from the natural source. In the case of artificial synthesis, the frequency of use of codons corresponding to respective amino acids may be changed depending on the host microorganism to be introduced.

When the nucleic acid encoding the enzyme is isolated from nature, the organism as the gene source is not particularly limited, and examples thereof include: acinetobacter such as Acinetobacter baylyi and Acinetobacter radioresistances; aerobacter genus such as Aerobacter cloacae; alcaligenes faecalis and other Alcaligenes; bacillus such as Bacillus badius, Bacillus magasterium and Bacillus roseus; brevibacterium iododinum and the like; corynebacterium species such as Corynebacterium acetoacidophilum, Corynebacterium acetoglutamicum, Corynebacterium ammoniagenes and Corynebacterium glutamicum; cupriavidus genus such as Cupriavidus metallichurans, Cupriavidus dicator, Cupriavidus numazuensis, Cupriavidus oxalatus, etc.; delftia acidovans and other Delftia species; escherichia coli, Escherichia fergusonii, and the like; hafnia alvei et al, Hafnia genus; microbacterium ammoniaphilum and other Microbacterium species; nocardia-like genera such as Nocardiaides albus; planomicrobium okanokoites and other zoonotic microorganisms; pseudomonas azotoformans, Pseudomonas chlororaphis, Pseudomonas fluorescens, Pseudomonas fragi, Pseudomonas putida, Pseudomonas reptilvora, etc.; rhizobia such as Rhizobium radiobacter; rhodosporidium such as Rhodosporidium toruloides; saccharomyces cerevisiae; serratia species such as Serratia entomophila, Serratia ficaria, Serratia fonticola, Serratia grimei, Serratia nematodiphila, Serratia odorifera, and Serratia plomutica; shimwellia blattae et al, genus Shimwellia; streptomyces species such as Streptomyces vinaceae, Streptomyces karnatakensis, Streptomyces olivaceus and Streptomyces vinaceae; yarrowia lipolytica and other Yarrowia lipolytica; yersinia genus such as Yersinia ruckeri; euglena gracilis and other Euglena genus; thermobifida fusca and other thermophilic bacteria, but preferably acinetobacter, Corynebacterium, Escherichia, Pseudomonas, Serratia, Euglena, and thermophilic bacteria.

In the present invention, the impairment of the functions of pyruvate kinase and phosphotransferase enzymes means the impairment of the activities of these enzymes. The method for impairing the function is not particularly limited, and it can be impaired by, for example: subjecting the gene encoding the enzyme to a genetic modification treatment by a genetic modification agent, ultraviolet irradiation or the like; subjecting a part or all of the base sequence to a treatment for damaging the base sequence by a site-specific mutagenesis method or the like; introducing a frameshift mutation into the base sequence; a stop codon is inserted into the nucleotide sequence. In addition, a gene may be damaged by removing all or a part of the nucleotide sequence or by replacing the nucleotide sequence with another nucleotide sequence using a gene recombination technique. Among these, a method in which a part or the whole of the base sequence is damaged is preferable.

Pyruvate kinase is classified under EC2.7.1.40 and is an enzyme that catalyzes a reaction in which phosphoenolpyruvate is dephosphorylated and converted into pyruvate and ATP. Specifically, examples thereof include pykF (NCBI-Protein ID: NP 416191, SEQ ID NO: 178), pykA (NCBI-Protein ID: NP 416368, SEQ ID NO: 179) derived from Escherichia coli strain K-12substr.MG1655, and pykF (SEQ ID NO: 180), pykA (SEQ ID NO: 181) derived from Serratia grimesii NBRC 13537. When the microorganism used in the present invention has 2or more genes encoding pyruvate kinase, it is preferable that all functions of pyruvate kinase are impaired, as shown in the metabolic pathway of scheme 2 below. Whether or not a polypeptide encoded by a gene possessed by a microorganism used in the present invention is pyruvate kinase can be determined by BLAST (basic Local Alignment Search tool) Search on the websites of NCBI (national Center for Biotechnology information) and KEGG (Kyoto Encyclopedia of Genes and genomes).

Phosphoenolpyruvate carboxylase, classified under EC4.1.1.49, is an enzyme that catalyzes the reaction that produces oxaloacetate and ATP from phosphoenolpyruvate, carbon dioxide, and ADP. Specifically, there may be mentioned pck (NCBI-Protein ID: NP 417862, SEQ ID NO: 182) derived from Escherichia coli strain K-12substr.MG1655, pckA 1 (SEQ ID NO: 183) derived from Serratia grimesii NBRC13537, pckA 2 (SEQ ID NO: 184), and the like.

PEP carboxylated kinase is physiologically responsible for the major reactions in the production of glucose from fatty acids during gluconeogenesis. The reaction catalyzed by PEP carboxylase is a reversible reaction, but during gluconeogenesis the reaction proceeds in the direction of conversion of oxaloacetate to PEP and carbon dioxide.

Whether or not the polypeptide encoded by the gene used in the present invention is PEP carboxylation kinase can be determined by BLAST (basic Local Alignment Search tool) Search on the websites of NCBI (national Center for Biotechnology information) and KEGG (Kyoto Encyclopedia of Genes and genomes), etc.

Japanese laid-open patent publication No. 2015-146810 describes: when a microorganism strain capable of producing adipic acid from acetyl-CoA and succinyl-CoA In high yield is prepared using In silico using a metabolic network model (network model), the impairment of the PEP carboxylase kinase gene is effective. In addition, Japanese laid-open patent publication No. 2015-504688 discloses: in the production of adipic acid biosynthesized via PEP, the activity of PEP carboxylase is enhanced with the aim of increasing the PEP pool (pool). That is, it is described that the reaction catalyzed by PEP carboxylase proceeds toward the production of PEP from oxaloacetate, and it is considered that the person skilled in the art can understand from this description that: since the enhancement of the enzyme activity is caused by the enhancement of the expression of the PEP carboxylase gene, it is expected that the yield of 3-hydroxyadipic acid, α -hydroadipic acid and/or adipic acid is reduced. However, in the present invention, it was found that, contrary to the expectation described above, when a genetically modified microorganism having an ability to produce 3-hydroxyadipic acid, α -hydrogenated adipic acid and/or adipic acid, and having a pyruvate kinase impaired ability, enhanced phosphoenolpyruvate carboxylase gene and expression of an enzyme gene catalyzing a reaction of reducing 3-oxoadipyl-CoA to 3-hydroxyadipyl-CoA is cultured, the production of 3-hydroxyadipic acid, α -hydrogenated adipic acid and/or adipic acid is increased.

The genetically modified microorganism of the present invention preferably has a function of a phosphotransferase enzyme. The phosphotransferase enzyme refers to phosphoenolpyruvate-dependent phosphotransferase system (PTS) (sometimes referred to as PTS enzyme in the present specification). As shown in the metabolic pathway of scheme 2 below, PTS is a main mechanism for uptake of hexose, hexitol, disaccharide, and other carbohydrates into cells. In PTS, carbohydrate is taken up into cells and converted into phosphate, and on the other hand, PEP, which is a phosphate donor, is converted into pyruvate.

acetyl-CoA, which is an intermediate in the biosynthesis of 3-hydroxyadipic acid, α -hydrohexadiene diacid, and/or adipic acid, can be synthesized from PEP via pyruvate utilizing the function of PTS enzymes.

[ chemical formula 2]

PTS enzymes are composed as follows: two common enzymes, phosphoenolpyruvate carbohydrate phosphotransferase I (phosphoenolpyruvate phosphate transferase enzyme I) and phosphocarrier protein HPr (phosphocarrier protein HPr), which function regardless of the type of carbohydrate; and membrane-bound carbohydrate specific osmolytes (enzyme II) specific for specific carbohydrates. The enzyme II further comprises sugar-specific IIA, IIB and IIC. The enzyme of enzyme II exists in a state in which single or plural enzymes are linked into a protein domain depending on the species of organism.

In the microorganism, phosphoenolpyruvate carbohydrate phosphotransferase I is encoded by the ptsI gene, phosphocarrier protein HPr is encoded by the ptsH gene, glucose-specific IIA is encoded by the crr gene, and glucose-specific IIB and IIC are both encoded by the ptsG gene.

The enzymes encoded by the ptsG Gene are classified under EC2.7.1.199 and called protein-Npi-phosphohistidine-D-glucose phosphotransferase (protein-Npi-phosphohistidine-D-glucose phosphotransferase), and PtsG (NCBI-Gene ID: 415619) derived from Escherichia coli strain K-12substr.MG1655 strain, PtsG (SEQ ID NO: 185) derived from Serratia grimesii NBRC13537 strain, and the like can be exemplified. Whether or not a polypeptide encoded by a gene of a microorganism used in the present invention is a PTS enzyme may be searched by BLAST on the website such as NCBI and KEGG.

In the present invention, 1 of the PTS enzyme genes may be damaged, or a plurality of the PTS enzyme genes may be damaged. Any of the above PTS enzyme genes may be impaired, but it is preferable to impair an enzyme gene involved in glucose uptake, and it is particularly preferable to impair ptsG gene. Specific examples of the ptsG Gene include ptsG (NCBI-Gene ID: 945651) derived from Escherichia coli strain K-12substr.MG1655, and ptsG (SEQ ID NO: 243) derived from Serratia grimesii NBRC 13537.

As described above, Escherichia coli is a microorganism having the ability to produce 3-hydroxyadipic acid and α -hydrogenated adipic acid, and Japanese patent application laid-open No. 2008-527991 describes: variants were made with the pykF and pykA encoding pyruvate kinase of Escherichia coli and the ptsG gene encoding phosphotransferase-like enzyme being impaired, and when the variants were cultured under anaerobic conditions, succinic acid yields increased and acetic acid and ethanol yields decreased. Here, as shown in the metabolic pathway of scheme 2 above, acetate and ethanol are compounds metabolized from acetyl-CoA. That is, in Japanese patent application laid-open No. 2008-527991, it is presumed that the supply amount of acetyl-CoA is decreased and the yields of acetic acid and ethanol are decreased by damaging ptsG, pykF and pykA genes of Escherichia coli.

On the other hand, as described above, the 3-hydroxyadipic acid, α -hydrogenated adipic acid and/or adipic acid produced in the method of the present invention is a compound obtained by metabolizing 3-oxoadipyl-CoA produced from acetyl-CoA and succinyl-CoA by reaction a through a plurality of reactions. Therefore, according to the disclosure of Japanese patent application laid-open No. 2008-527991, when genes of pyruvate kinase and phosphotransferase enzymes are damaged, the supply amount of acetyl-CoA is decreased, and thus it is expected that the yield of 3-hydroxyadipic acid, α -hydrogenated adipic acid and/or adipic acid is also decreased. However, in the present invention, contrary to the expectation described above, in the genetically modified microorganism in which the activity of phosphoenolpyruvate carboxylase and the activity of an enzyme catalyzing the reaction of reducing 3-oxoadipyl-CoA to 3-hydroxyadipyl-CoA are enhanced, the yield of 3-hydroxyadipic acid, α -hydrogenated adipic acid and/or adipic acid is increased and the yield of acetic acid and ethanol is also increased by damaging the genes of pyruvate kinase and phosphotransferase enzymes.

The genetically modified microorganism of the present invention may be cultured in a medium containing a carbon source that is generally available to the microorganism as a fermentation raw material, preferably in a liquid medium. A medium may be used which contains a nitrogen source, inorganic salts, and, if necessary, organic micronutrients such as amino acids and vitamins in addition to the carbon source utilizable by the genetically modified microorganism. As long as the nutrient source is contained, either a natural medium or a synthetic medium can be used.

The fermentation raw material refers to a raw material which can be metabolized by the genetically modified microorganism. "metabolism" refers to the conversion of a chemical substance taken up by a microorganism from outside the cell or produced by another chemical substance inside the cell into another chemical substance by an enzymatic reaction. As the carbon source, saccharides are preferably used. Specific examples of the saccharides include: monosaccharides such as glucose, sucrose, fructose, galactose, mannose, xylose, and arabinose; disaccharides obtained by combining these monosaccharides; polysaccharides; and a starch saccharification liquid, molasses, cellulose-containing biomass saccharification liquid and the like containing the same.

The carbon sources listed above may be used alone or in combination, but it is particularly preferable to culture the cells in a medium containing glucose. In addition of the carbon source, the concentration of the carbon source in the medium is not particularly limited, and may be appropriately set according to the kind of the carbon source. The preferred concentration of glucose is 5g/L to 300 g/L.

As the nitrogen source for culturing the genetically modified microorganism, for example, ammonia gas, ammonia water, ammonium salts, urea, nitrates, and other auxiliary organic nitrogen sources: for example, oil residues, soybean hydrolysate, casein hydrolysate, other amino acids, vitamins, corn steep liquor, yeast or yeast extract, peptides such as meat extract and tryptone, various fermented cells and hydrolysates thereof, and the like. The concentration of the nitrogen source in the medium is not particularly limited, but is preferably 0.1g/L to 50 g/L.

As the inorganic salts used for culturing the genetically modified microorganism, for example, phosphate, magnesium salt, calcium salt, iron salt, manganese salt and the like can be added as appropriate.

The culture conditions of the genetically modified microorganism for producing 3-hydroxyadipic acid, α -hydrohexadiene diacid and/or adipic acid can be appropriately adjusted or selectively set depending on the type of the genetically modified microorganism, external conditions, and the like, and the medium, culture temperature, stirring speed, pH, aeration amount, inoculation amount, and the like of the above components.

The pH range during culture is not particularly limited as long as the genetically modified microorganism can grow, and is preferably 5 to 8, more preferably 5.5 to 6.8.

The range of aeration conditions during the culture is not particularly limited as long as 3-hydroxyadipic acid, α -hydrohexadiene diacid and/or adipic acid can be produced, but for good growth of the microorganism variant, it is preferable that oxygen remains in the gas phase and/or the liquid phase in the culture vessel at least at the start of the culture.

In the case of foaming during liquid culture, mineral oil, silicone oil, and an antifoaming agent such as a surfactant may be appropriately mixed into the medium.

After 3-hydroxyadipic acid, alpha-hydrogenated adipic acid, and/or adipic acid are produced in the culture of the microorganism to recoverable amounts, the product produced can be recovered. For the recovery of the product produced, for example, the isolation may be carried out by stopping the culture at a time when the accumulation amount is moderately increased, and following a general method of extracting the fermentation product from the culture. Specifically, the bacterial cells are separated by centrifugation, filtration, or the like, and the product is separated from the culture by column chromatography, ion exchange chromatography, activated carbon treatment, crystallization, membrane separation, distillation, or the like. More specifically, there may be mentioned: a method of recovering a precipitate by adding an acid component to a salt of the product; a method in which a culture is concentrated with a reverse osmosis membrane or a rotary evaporator to remove water and increase the concentration of the product, and then the product and/or the salt of the product are crystallized by cooling crystallization or adiabatic crystallization, and then the product and/or the salt of the product are crystallized by centrifugation or filtration; a method of adding an alcohol to the culture to convert the product into an ester, recovering the ester of the product by distillation, and then obtaining the product by hydrolysis, but not limited thereto. These recovery methods can be appropriately selected and optimized according to the physical properties of the product.

Examples

The present invention will be described in detail below with reference to examples.

Reference example 1

Preparation of plasmids expressing an enzyme catalyzing a reaction producing 3-oxoadipyl-CoA and coenzyme A (reaction A), an enzyme catalyzing a reaction producing 3-hydroxyadipic acid from 3-hydroxyadipyl-CoA (reaction E) and a reaction producing α -hydroadipic acid from 2, 3-dehydroadipyl-CoA (reaction F), and the polypeptides of SEQ ID Nos. 1, 2,3, 4, 5, 6 and 7

The autonomously replicable vector pBBR1MCS-2 in E.coli (ME Kovach, (1995), Gene 166: 175-. In order to incorporate the constitutive expression promoter into this vector, primers (SEQ ID NOS: 187 and 188) for PCR amplification of the upstream region 200b (SEQ ID NO: 186) of gapA (NCBI Gene ID: NC 000913.3) were designed using the genomic DNA of Escherichia coli K-12MG1655 as a template, and PCR was carried out according to a conventional method. The resulting fragment was ligated with pBBR1MCS-2/XhoI using In-Fusion HD Cloning Kit ("タカラバイオ Co., Ltd.), and introduced into Escherichia coli strain DH 5. alpha. The plasmid was extracted from the resulting recombinant Escherichia coli strain, and the plasmid whose nucleotide sequence was confirmed by a conventional method was pBBR1MCS-2:: Pgap. Next, the pBBR1MCS-2:: Pgap was cleaved with ScaI to obtain pBBR1MCS-2:: Pgap/ScaI. In order to amplify the Gene encoding the enzyme catalyzing reaction A, primers (SEQ ID NOS: 190 and 191) for PCR amplification of the entire length of the acyltransferase Gene pcaF (NCBI Gene ID: 1041755, SEQ ID NO: 189) were designed using genomic DNA of Pseudomonas putida KT2440 as a template, and PCR was carried out according to a conventional method. The resulting fragment was ligated with pBBR1 MCS-2:Pgap/ScaI using the In-Fusion HD Cloning Kit and introduced into Escherichia coli strain DH 5. alpha. This plasmid was extracted from the resulting recombinant strain, and the plasmid whose nucleotide sequence was confirmed by a conventional method was pBBR1MCS-2:: AT. Next, the pBBR1MCS-2:: AT was cleaved with HpaI to obtain pBBR1MCS-2:: AT/HpaI. In order to amplify the genes encoding the enzymes catalyzing reaction D and reaction E, primers (SEQ ID NOS: 194, 195) for PCR amplification of the full-length continuous sequence comprising the CoA transferase genes pcaI and pcaJ (NCBI Gene ID: 1046613, 1046612, SEQ ID NOS: 192, 193) were designed using the genomic DNA of Pseudomonas putida KT2440 strain as a template, and PCR reaction was carried out according to a conventional method. The resulting fragment was ligated with pBBR1MCS-2:: AT/HpaI using the In-Fusion HD Cloning Kit and introduced into Escherichia coli strain DH5 α. This plasmid was extracted from the resulting recombinant strain, and the plasmid whose nucleotide sequence was confirmed by a conventional method was pBBR1MCS-2:: ATCT.

The pBBR1MCS-2: ATCT/ScaI was cleaved with ScaI to obtain pBBR1MCS-2: ATCT/ScaI. In order to amplify the nucleic acid encoding the polypeptide of SEQ ID NO. 1, primers (SEQ ID NO. 196 and 197) for amplifying the nucleic acid of SEQ ID NO. 87 were designed using genomic DNA of Serratia marcocens ATCC13880 as a template, and PCR was performed according to a conventional method. In order to amplify the nucleic acid encoding the polypeptide of SEQ ID NO. 2, primers (SEQ ID NOS: 198 and 199) for amplifying the nucleic acid of SEQ ID NO. 88 were designed using genomic DNA of Serratia nematodiphila DSM21420 strain as a template, and PCR was performed according to a conventional method. In order to amplify the nucleic acid encoding the polypeptide of SEQ ID NO. 3, primers (SEQ ID NO. 200 and 201) for amplifying the nucleic acid of SEQ ID NO. 89 were designed using genomic DNA of Serratia plymuthica NBRC102599 strain as a template, and PCR was performed according to a conventional method. In order to amplify the nucleic acid encoding the polypeptide of SEQ ID NO. 4, primers (SEQ ID NOS: 202 and 203) for amplifying the nucleic acid of SEQ ID NO. 90 were designed using genomic DNA of Serratia proteinacalans 568 strain as a template, and PCR was performed according to a conventional method. In order to amplify the nucleic acid encoding the polypeptide of SEQ ID NO. 5, primers (SEQ ID NO. 204 and 205) for amplifying the nucleic acid of SEQ ID NO. 91 were designed using genomic DNA of Serratia urealytica Lr5/4 strain as a template, and PCR was performed according to a conventional method. In order to amplify the nucleic acid encoding the polypeptide of SEQ ID NO. 6, primers (SEQ ID NO. 206 and 207) for amplifying the nucleic acid of SEQ ID NO. 92 were designed using genomic DNA of Serratia sp.BW106 strain as a template, and PCR was performed according to a conventional method. In order to amplify the nucleic acid encoding the polypeptide of SEQ ID NO. 7, primers (SEQ ID NO. 208 and 209) for amplifying the nucleic acid of SEQ ID NO. 93 were designed using genomic DNA of Serratia lipofectens FK01 strain as a template, and PCR was performed according to a conventional method. The fragments obtained were ligated to pBBR1MCS-2:ATCT/ScaI using the In-Fusion HD Cloning Kit ("タカラバイオ Co., Ltd.), and introduced into Escherichia coli strain DH 5. alpha. The plasmid was extracted from the obtained recombinant strain, and the nucleotide sequence was confirmed by a conventional method.

The plasmid for expressing the polypeptide of the sequence number 1 is set as pBBR1MCS-2: ATCTOR 1; the plasmid used for expressing the polypeptide of the sequence number 2 is set as pBBR1MCS-2: ATCTOR 2; the plasmid used for expressing the polypeptide of the sequence number 3 is set as pBBR1MCS-2: ATCTOR 3; the plasmid used for expressing the polypeptide of the sequence number 4 is set as pBBR1MCS-2: ATCTOR 4; the plasmid used for expressing the polypeptide of the sequence number 5 is set as pBBR1MCS-2: ATCTOR 5; the plasmid used for expressing the polypeptide of the sequence number 6 is set as pBBR1MCS-2: ATCTOR 6; the plasmids used for expressing the polypeptide of SEQ ID NO. 7 were designated "pBBR 1MCS-2:: ATCTOR 7", and these plasmids are shown in Table 4.

[ Table 4]

Reference example 2

Preparation of plasmid for expressing enzyme catalyzing reaction of producing 2, 3-dehydroadipoyl-CoA from 3-hydroxyadipoyl-CoA (reaction C)

The autonomously replicable expression vector pMW119 in Escherichia coli (manufactured by "ニッポンジーン") was excised using SacI to obtain pMW 119/SacI. In order to incorporate the constitutive expression promoter into this vector, primers (SEQ ID NOS: 210 and 211) for PCR amplification of the upstream region 200b (SEQ ID NO: 186) of gapA (NCBI Gene ID: NC 000913.3) were designed using the genomic DNA of Escherichia coli K-12MG1655 as a template, and PCR was carried out according to a conventional method. The resulting fragment was ligated with pMW119/SacI using In-Fusion HD Cloning Kit ("タカラバイオ Co., Ltd.), and introduced into Escherichia coli strain DH 5. alpha. The plasmid was extracted from the resulting recombinant Escherichia coli strain, and the plasmid whose nucleotide sequence was confirmed by a conventional method was designated pMW119:: Pgap. Next, pMW119:: Pgap was cleaved with SphI to give pMW119:: Pgap/SphI. In order to amplify the Gene encoding the enzyme catalyzing reaction C, primers (SEQ ID NOS: 212 and 213) for PCR amplification of the full length of enoyl-CoA hydratase Gene paaF (NCBI Gene ID: 1046932, SEQ ID NO: 176) were designed using genomic DNA of Pseudomonas putida KT2440 strain as a template, and PCR reaction was carried out according to a conventional method. The resulting fragment was ligated with pMW 119:Pgap/SphI using In-Fusion HD Cloning Kit ("タカラバイオ Co., Ltd.), and introduced into Escherichia coli strain DH5 α. The plasmid was extracted from the obtained recombinant strain, and the nucleotide sequence was confirmed by a conventional method. The resulting plasmid was designated "pMW 119:: EH".

Reference example 3

Preparation of plasmids expressing an enzyme catalyzing a reaction (reaction A) producing 3-oxoadipyl-CoA and coenzyme A from acetyl-CoA and succinyl-CoA, an enzyme catalyzing a reaction (reaction G) producing adipic acid from adipyl-CoA, and the polypeptides of SEQ ID NOS.1, 2,3, 4, 5, 6 and 7

In order to amplify the Gene encoding the enzyme catalyzing reaction G, primers (SEQ ID NOS: 241, 242) for PCR amplification of the full-length continuous sequence containing CoA transferase genes dcaI and dcaJ (NCBI Gene ID: CR543861.1, SEQ ID NOS: 239, 240) were designed using the genomic DNA of Acinetobacter baylyi ADP1 strain as a template, and PCR reaction was carried out according to a conventional method. The fragments obtained by cleaving the pBBR1MCS-2:: ATCTOR1, pBBR1MCS-2:: ATCTOR2, pBBR1MCS-2:: ATCTTOR 3, pBBR1MCS-2:: ATCTTOR 4, pBBR1MCS-2:: ATCTTOR 5, pBBR1MCS-2:: ATCTTOR 6, pBBR1MCS-2:: ATCTOR7, were ligated to the fragment prepared In reference example 1 using the In-Fusion HD Cloning Kit, and introduced into Escherichia coli DH5 α. The plasmids were extracted from the resulting recombinant strains, and the plasmids whose nucleotide sequences were confirmed by a conventional method were pBBR1MCS-2:: ATCT2OR1, pBBR1MCS-2:: ATCT2OR2, pBBR1MCS-2:: ATCT2OR3, pBBR1MCS-2:: ATCT2OR4, pBBR1MCS-2:: ATCT2OR5, pBBR1MCS-2:: ATCT2OR6, pBBR1MCS-2:: ATCT2OR7, respectively.

Reference example 4

Preparation of plasmid for expressing enzyme catalyzing reaction for producing 2, 3-dehydroadipoyl-CoA from 3-hydroxyadipoyl-CoA (reaction C) and reaction for producing adipoyl-CoA from 2, 3-dehydroadipoyl-CoA (reaction D)

pMW119:: EH was cut with HindIII to give pMW119:: EH/HindIII. In order to amplify the gene encoding the enzyme catalyzing reaction D, primers (SEQ ID NOS: 215 and 216) for PCR amplification of the entire length of dcaA (NCBI-Protein ID: AAL09094.1, SEQ ID NO: 214) derived from Acinetobacter baylyi ADP1 strain were designed, and PCR reaction was carried out according to a conventional method. The resulting fragment was ligated with pMW119:, EH/HindIII using In-Fusion HD Cloning Kit ("タカラバイオ Co., Ltd.), and introduced into Escherichia coli strain DH5 α. The plasmid was extracted from the resulting recombinant strain, and the plasmid whose nucleotide sequence was confirmed by a conventional method was designated pMW119:: EHER.

Reference example 5

Preparation of plasmids expressing PEP carboxylase, an enzyme catalyzing a reaction (reaction A) for producing 3-oxoadipyl-CoA and coenzyme A, an enzyme catalyzing a reaction (reaction E) for producing 3-hydroxyadipic acid from 3-hydroxyadipyl-CoA and a reaction (reaction F) for producing alpha-hydroadipyl-adipyl-diacid from 2, 3-dehydroadipyl-CoA, and the polypeptides described in SEQ ID Nos. 1, 2,3, 4, 5, 6 and 7

In order to assemble a promoter which constitutively expresses PEP carboxylase, primers (SEQ ID NOS: 217 and 218) for PCR amplification of the upstream region 200b (SEQ ID NO: 186) of gapA (NCBI Gene ID: NC 000913.3) were designed using genomic DNA of Escherichia coli K-12MG1655 as a template, and PCR was carried out according to a conventional method. The fragments obtained by cleaving pBBR1MCS-2:: ATCTOR1, pBBR1MCS-2:: ATCTOR2, pBBR1MCS-2:: ATCTTOR 3, pBBR1MCS-2:: ATCTTOR 4, pBBR1MCS-2:: ATCTTOR 5, pBBR1MCS-2:: ATCTTOR 6, pBBR1MCS-2:: ATCTOR7, were ligated to SacI prepared In reference example 1 using the In-Fusion HD Cloning Kit, and the fragments obtained by cleaving were introduced into Escherichia coli DH5 α. The plasmids were extracted from the resulting recombinant strain and the nucleotide sequences of the plasmids were confirmed by a conventional method, respectively, pBBR1MCS-2: ATCTTOR 1Pgap, pBBR1MCS-2: ATCTTOR 2Pgap, pBBR1MCS-2: ATCTTOR 3Pgap, pBBR1MCS-2: ATCTTOR 4Pgap, pBBR1MCS-2: ATCTTOR 5Pgap, pBBR1MCS-2: ATCTTOR 6Pgap, pBBR1MCS-2: ATCTTOR 7 Pgap. Then, in order to amplify the gene encoding PEP carboxylase, primers (seq id nos 220 and 221) for PCR amplification of the full-length continuous sequence including the PEP carboxylase gene (seq id no 219) were designed using the genomic DNA of Serratia grimesii NBRC13537 strain as a template, and PCR reaction was performed according to a conventional method. The resulting fragments were ligated with SacI by using the In-Fusion HD Cloning Kit and pBBR1MCS-2:, ATCTTOR 1Pgap, pBBR1MCS-2:, ATCTTOR 2Pgap, pBBR1MCS-2:, ATCTTOR 3Pgap, pBBR1MCS-2:, ATCTTOR 4Pgap, pBBR1MCS-2:, ATCTTOR 5Pgap, pBBR1MCS-2:, ATCTTOR 6Pgap, pBBR1MCS-2:, ATCTTOR 7Pgap, respectively, and introduced into Escherichia coli DH 5. alpha. The plasmids were extracted from the resulting recombinant strain, and the base sequences of the plasmids determined by the conventional method were pBBR1MCS-2:, ATCTTOR 1PCK, pBBR1MCS-2:, ATCTTOR 2PCK, pBBR1MCS-2:, ATCTTOR 3PCK, pBBR1MCS-2:, ATCTTOR 4PCK, pBBR1MCS-2:, ATCTTOR 5PCK, pBBR1MCS-2:, ATCTTOR 6PCK, pBBR1MCS-2:, and ATCTTOR 7PCK, respectively.

Reference example 6

Preparation of plasmids for expressing PEP carboxylase, an enzyme catalyzing a reaction (reaction A) from acetyl-CoA and succinyl-CoA to 3-oxoadipyl-CoA and coenzyme A, an enzyme catalyzing a reaction (reaction G) from adipyl-CoA to adipic acid, and the polypeptides of SEQ ID Nos. 1, 2,3, 4, 5, 6 and 7

The upstream region 200b of gapA (NCBI Gene ID: NC 000913.3) using the genomic DNA of Escherichia coli K-12MG1655 as a template and the PEP-carboxylated kinase Gene from Serratia grimesii NBRC13537 strain were inserted into pBBR1MCS-2:: ATCT2OR1, pBBR1MCS-2:: ATCT2OR2, pBBR1MCS-2:: ATCT2OR3, pBBR1MCS-2:: ATCT2OR4, pBBR1MCS-2:: ATCT2OR5, pBBR1MCS-2:: T2OR6, pBATCR 1 MCS-2:ATCT2 OR7, using the same method and plasmid as in reference example 5. The plasmids thus obtained were designated pBBR1MCS-2: ATCT2OR1PCK, pBBR1MCS-2: ATCT2OR2PCK, pBBR1MCS-2: ATCT2OR3PCK, pBBR1MCS-2: ATCT2OR4PCK, pBBR1MCS-2: ATCT2OR5PCK, pBBR1MCS-2: ATCT2OR6PCK, pBBR1MCS-2: ATCT2OR7PCK, respectively.

Example 1

Preparation of a mutant of microorganism belonging to genus Serratia in which pyruvate kinase function is impaired

A mutant of a microorganism belonging to the genus Serratia, in which the function of pyruvate kinase is impaired, is produced by disrupting pykF and pykA genes encoding pyruvate kinase of the microorganism belonging to the genus Serratia.

Methods of impairment of pykF and pykA can be found in Proc Natl Acad Sci USA.2000Jun 6; 97 (97) (6640) and 6645.

Preparation of a variant of a microorganism belonging to genus Serratia with impaired pykF

PCR was carried out using pKD4 as a template and the oligomeric DNAs of SEQ ID Nos. 222 and 223 as primers to obtain a PCR fragment of 1.6kb in sequence length for damaging pykF. An ampicillin-resistant strain was obtained by introducing pKD46, which is an expression plasmid for FRT recombinase (FRT recombinant), into Serratia grimaceii NBRC 13537. The resulting strain was inoculated into 5mL of LB medium containing 500. mu.g/mL ampicillin, and shake-cultured at 30 ℃ for 1 day. 0.5mL of the culture was inoculated into 50mL of LB medium containing 500. mu.g/mL of ampicillin and 50mM of arabinose, and cultured by rotation at 30 ℃ for 2 hours. After the culture solution was cooled in ice for 20 minutes, the cells were washed 3 times with 10% (w/w) glycerol. The washed pellet was suspended in 100. mu.L of 10% (w/w) glycerol, mixed with 5. mu.L of PCR fragment, and then cooled in an electroporation Cuvette (Cuvette) for 10 minutes. After electroporation (3kV, 200. omega. and 25. mu.F) was performed using Gene pulser (Bio-rad), 1mL of SOC medium was added immediately, and shaking culture was performed at 30 ℃ for 2 hours. All were spread on LB agar medium containing 25. mu.g/mL kanamycin and incubated at 30 ℃ for 1 day. Colony direct PCR was performed using the obtained kanamycin-resistant strain, and the damage of the target gene and the insertion of the kanamycin-resistant gene were confirmed from the band length. The primers used were the oligo DNAs of SEQ ID Nos. 224 and 226.

Subsequently, the kanamycin-resistant strain was inoculated into 5mL of LB medium, and pKD46 was detached by 2 subcultures at 37 ℃ to obtain an ampicillin-sensitive strain. An ampicillin-resistant strain was again obtained by introducing pCP20 into the ampicillin-sensitive strain. The obtained strain was cultured at 40 ℃ and then subjected to colony direct PCR, and the detachment of the kanamycin resistance gene was confirmed from the band length. The primers used were oligo DNAs of SEQ ID Nos. 225 and 226. The kanamycin-sensitive strain was inoculated into 5mL of LB medium, and pCP20 was exfoliated by 2 subcultures at 37 ℃. The resulting strain was designated as Serratia grinesi NBRC 13537. delta. pykF.

Preparation of a variant of a microorganism of the genus Serratia with impaired pykA

PCR was carried out using pKD4 as a template and the oligo DNAs of SEQ ID Nos. 227 and 228 as primers to obtain a pykA-compromised PCR fragment of 1.6kb in sequence length.

The Serratia grinesii NBRC13537 Δ pykF strain was impaired in pykA by the same method as that for the production of the pykF impaired strain. The strain was introduced with pKD46 and then a pykA-damaging PCR fragment was introduced. Colony direct PCR was performed using the obtained kanamycin-resistant strain, and the damage of the target gene and the insertion of the kanamycin-resistant gene were confirmed from the band length. The primers used were the oligo DNAs of SEQ ID Nos. 224 and 230.

Subsequently, pKD46 was released to obtain an ampicillin-sensitive strain. An ampicillin-resistant strain was again obtained by introducing pCP20 into the ampicillin-sensitive strain. The obtained strain was subjected to colony direct PCR, and the shedding of the kanamycin resistance gene was confirmed from the band length. The primers used were the oligo DNAs of SEQ ID Nos. 229 and 230. pCP20 was detached from the kanamycin-sensitive strain. The obtained strain was designated as Sg. DELTA.PP.

Example 2

Preparation of a variant of a microorganism belonging to the genus Serratia into which a plasmid is introduced, which impairs the function of pyruvate kinase and expresses PEP carboxylase and an enzyme catalyzing reactions A, B, E and F

The plasmids prepared in reference example 1 were introduced into each of the Sg.DELTA.PP prepared in example 1 to prepare a microorganism variant of Serratia.

Sg.DELTA.PP was inoculated into 5mL of LB medium and shake-cultured at 30 ℃ for 1 day. 0.5mL of the culture broth was inoculated into 5mL of LB medium and shake-cultured at 30 ℃ for 2 hours. After the culture solution was cooled in ice for 20 minutes, the cells were washed 3 times with 10% (w/w) glycerol. The washed pellet was suspended in 100. mu.L of 10% (w/w) glycerol, and mixed with 1. mu.L of pBBR1MCS-2:: ATCTRO 1PCK, pBBR1MCS-2:: ATCTRO 2PCK, pBBR1MCS-2:: ATCTRO 3PCK, pBBR1MCS-2:: ATCTRO 4PCK, pBBR1MCS-2:: ATCTRO 5PCK, pBBR1MCS-2:: ATCTRO 6PCK or pBBR1MCS-2:: ATCTRO 7PCK, followed by ice-cooling in an electroporation cuvette for 10 minutes. After electroporation (3kV, 200. omega. and 25. mu.F) was performed using Gene pulser (Bio-rad), 1mL of SOC medium was added immediately, and shaking culture was performed at 30 ℃ for 1 hour. 50 μ L was spread on LB agar medium containing 25 μ g/mL kanamycin, and incubated at 30 ℃ for 1 day. The obtained strains were designated as Sg. delta. PP/3HA1PCK, Sg. delta. PP/3HA2PCK, Sg. delta. PP/3HA3PCK, Sg. delta. PP/3HA4PCK, Sg. delta. PP/3HA5PCK, Sg. delta. PP/3HA6PCK, and Sg. delta. PP/3HA7PCK, respectively.

Reference example 7

Preparation of a variant of a microorganism belonging to the genus Serratia into which a plasmid expressing PEP carboxylase and an enzyme catalyzing reactions A, B, E and F was introduced without impairing the function of pyruvate kinase

In the same manner as in example 2, pBBR1MCS-2:, ATCTOR1PCK, pBBR1MCS-2:, ATCTOR2PCK, pBBR1MCS-2:, ATCTOR3PCK, pBBR1MCS-2:, ATCTOR4PCK, pBBR1MCS-2:, ATCTOR5PCK, pBBR1MCS-2:, ATCTOR6PCK, or pBBR1MCS-2:, ATCTOR7PCK was introduced into Serratia grimesii NBRC 13537. In addition, as a control, a strain in which pBBR1MCS-2 was introduced as a blank vector into Serratia grimesii NBRC13537 was prepared. The resulting strains were designated as Sg/3HA1PCK, Sg/3HA2PCK, Sg/3HA3PCK, Sg/3HA4PCK, Sg/3HA5PCK, Sg/3HA6PCK, Sg/3HA7PCK, and Sg/pBBR, respectively (negative control).

Reference example 8

Preparation of a variant of a microorganism belonging to the genus Serratia into which a plasmid is introduced, which impairs the function of pyruvate kinase and expresses an enzyme that catalyzes reactions A, B, E and F

In the same manner as in example 2, pBBR1MCS-2: ATCTOR1, pBBR1MCS-2: ATCTOR2, pBBR1MCS-2: ATCTOR3, pBBR1MCS-2: ATTOR 3, pBBR1MCS-2: ATCTOR4, pBBR1MCS-2: ATCTOR5, pBBR1MCS-2: ATTOR 6, or pBBR1MCS-2: ATCTOR7 was introduced into Sg.DELTA.PP. In addition, as a control, a strain was prepared in which pBBR1MCS-2 was introduced as a blank vector into Sg. DELTA.PP. The resulting strains were designated as Sg. DELTA. PP/3HA1, Sg. DELTA. PP/3HA2, Sg. DELTA. PP/3HA3, Sg. DELTA. PP/3HA4, Sg. DELTA. PP/3HA5, Sg. DELTA. PP/3HA6, Sg. DELTA. PP/3HA7, and Sg. DELTA. PP/pBBR (negative control), respectively.

Example 3

Assay for the production of 3-hydroxyadipic acid and alpha-hydrohexadiene diacid using a variant of a microorganism of the genus Serratia having impaired pyruvate kinase function and enhanced PEP carboxylase kinase activity

Production tests of 3-hydroxyadipic acid and α -hydrohexadiene diacid were carried out using the microorganism variants of Serratia prepared in example 2.

The mutant produced in example 2, which was a platinum loop, was inoculated into 5mL of a medium I (10g/L tryptone for bacteria (manufactured by Difco Laboratories), 5g/L yeast extract for bacteria (manufactured by Difco Laboratories), 5g/L sodium chloride, 25. mu.g/mL kanamycin) (a 18mm glass tube, an aluminum plug) adjusted to pH7, and cultured with shaking at 30 ℃ for 24 hours for 120 min-1. 0.25mL of this culture medium was added to 5mL of medium II (50g/L glucose, 1g/L ammonium sulfate, 50mM potassium phosphate, 0.025g/L magnesium sulfate, 0.0625mg/L iron sulfate, 2.7mg/L manganese sulfate, 0.33mg/L calcium chloride, 1.25g/L sodium chloride, 2.5g/L tryptone for bacteria, 1.25g/L yeast extract for bacteria, 25. mu.g/mL kanamycin) (phi 18mM glass tube, aluminum plug) adjusted to pH6.5, and shake-cultured at 30 ℃ for 24 hours at 120 min-1.

Quantitative analysis of substrates and products

The supernatant obtained by centrifugation of the cells from the culture broth was subjected to membrane treatment using Millex-GV (0.22 μm, PVDF, manufactured by Merck), and the permeate was analyzed by the following method, whereby the concentrations of 3-hydroxyadipic acid, α -hydrohexadiene diacid, and other products accumulated in the culture supernatant and of saccharides remaining unused in the culture medium were quantified. Further, the yields of 3-hydroxyadipic acid and α -hydrogenated adipic acid calculated from the results using the following formula (2) are shown in table 5.

However, in the quantitative analysis by LC-MS/MS, the case of 0.1mg/L or less is set to be the detection limit or less, and is shown as N.D in the table.

Yield (%). yield of product formation (mol)/sugar consumption (mol). times.100. formula (2)

Quantitative analysis of 3-hydroxyadipic acid and alpha-hydrohexadiene diacid Using LC-MS/MS

HPLC: 1290Infinity (Agilent Technologies, Inc.)

Column: synergi hydro-RP (manufactured by Phenomenex Co., Ltd.), length of 100mm, inner diameter of 3mm, and particle diameter of 2.5 μm

Mobile phase: 0.1% aqueous formic acid/methanol 70/30

Flow rate: 0.3 mL/min

Column temperature: 40 deg.C

An LC detector: 1260DAD VL + (210nm)

MS/MS: Triple-Quad LC/MS (manufactured by Agilent Technologies Co., Ltd.)

An ionization method: ESI negative mode.

Quantitative analysis of organic acids by HPLC

HPLC: LC-10A ("manufactured)

Column: shim-pack SPR-H ("Shimadzu ジーエルシー"), length 250mm, inner diameter 7.8mm, particle diameter 8 μm

Shim-pack SCR-101H (manufactured by Shimadzu ジーエルシー), length 250mm, inner diameter 7.8mm, particle diameter 10 μm

Mobile phase: 5mM p-toluenesulfonic acid

Reaction solution: 5mM p-toluenesulfonic acid, 0.1mM EDTA, 20mM Bis-Tris

Flow rate: 0.8 mL/min

Column temperature: 45 deg.C

A detector: CDD-10Avp ("manufactured made" separately)

Quantitative analysis of sugars and alcohols by HPLC

HPLC: shimazu project ("manufactured post)

Column: shodex soot SH1011 ("Showa electric Co., Ltd."), 300mm in length, 8mm in inner diameter, and 6 μm in particle diameter

Mobile phase: 0.05M aqueous sulfuric acid solution

Flow rate: 0.6 mL/min

Column temperature: 65 deg.C

A detector: RID-10A ("manufactured").

Comparative example 1

Assay for the production of 3-hydroxyadipic acid and alpha-hydrohexadiene diacid using a variant of a microorganism of the genus Serratia which does not impair the function of pyruvate kinase and which has enhanced PEP carboxylase activity

The mutants prepared in reference example 7 were cultured in the same manner as in example 3. The concentrations of 3-hydroxyadipic acid, α -hydrogenated adipic acid, and other products accumulated in the culture supernatant and of the sugars remaining unused in the medium were quantified. Further, the yields of 3-hydroxyadipic acid and α -hydrogenated adipic acid calculated from the results using the above formula (2) are shown in table 5.

Comparative example 2

Assay for the production of 3-hydroxyadipic acid and alpha-hydrohexadiene diacid using a variant of a microorganism of the genus Serratia which has impaired pyruvate kinase function and in which PEP carboxylase kinase activity has not been potentiated

The mutants prepared in reference example 8 were cultured in the same manner as in example 3. The concentrations of 3-hydroxyadipic acid, α -hydrogenated adipic acid, and other products accumulated in the culture supernatant and of the sugars remaining unused in the medium were quantified. Further, the yields of 3-hydroxyadipic acid and α -hydrogenated adipic acid calculated from the results using the above formula (2) are shown in table 5.

When the results of comparative examples 1 and 2 are compared with the results of example 3, it is understood that: by impairing the function of pyruvate kinase of a microorganism belonging to the genus Serratia and enhancing the activity of PEP carboxylase, the yields of 3-hydroxyadipic acid and alpha-hydrohexadiene diacid can be improved.

[ Table 5]

Example 4

Preparation of variant of Escherichia coli having impaired function of pyruvate kinase

The genes pykF and pykA encoding pyruvate kinase of escherichia coli were disrupted to create variants of escherichia coli with impaired pyruvate kinase function, and the disruption method for pykF and pykA was performed according to Proc Natl Acad Sci usa.2000jun 6; 97 (97) (6640) and 6645.

Preparation of variants of Escherichia coli having pykF-impaired Gene

PCR was carried out using pKD4 as a template and the oligomeric DNA of SEQ ID Nos. 231 and 232 as primers to obtain a 1.6kb PCR fragment of sequence length for pykF disruption.

pKD46, which is an expression plasmid for FRT recombinase, was introduced into Escherichia coli MG1655 strain to obtain ampicillin-resistant strain. The resulting strain was inoculated into 5mL of LB medium containing 100. mu.g/mL ampicillin, and shake-cultured at 30 ℃ for 1 day. 0.5mL of the culture was inoculated into 50mL of LB medium containing 100. mu.g/mL of ampicillin and 50mM of arabinose, and rotary-cultured at 30 ℃ for 2 hours. After the culture solution was cooled in ice for 20 minutes, the cells were washed 3 times with 10% (w/w) glycerol. The washed pellet was suspended in 100. mu.L of 10% (w/w) glycerol, mixed with 5. mu.L of PCR fragment, and then cooled in an electroporation cuvette for 10 minutes. After electroporation (3kV, 200. omega. and 25. mu.F) was performed using Gene pulser (Bio-rad), 1mL of SOC medium was added immediately, and shaking culture was performed at 30 ℃ for 2 hours. All were spread on LB agar medium containing 25. mu.g/mL kanamycin and incubated at 30 ℃ for 1 day. Colony direct PCR was performed using the obtained kanamycin-resistant strain, and the damage of the target gene and the insertion of the kanamycin-resistant gene were confirmed from the band length. The primers used were the oligo DNAs of SEQ ID Nos. 224 and 234.

Subsequently, the kanamycin-resistant strain was inoculated into 5mL of LB medium, and pKD46 was detached by 2 subcultures at 37 ℃ to obtain an ampicillin-sensitive strain. An ampicillin-resistant strain was again obtained by introducing pCP20 into the ampicillin-sensitive strain. The obtained strain was cultured at 40 ℃ and then subjected to colony direct PCR, and the detachment of the kanamycin resistance gene was confirmed from the band length. The primers used were the oligo DNAs of SEQ ID Nos. 233 and 234. The kanamycin-sensitive strain was inoculated into 5mL of LB medium, and pCP20 was exfoliated by 2 subcultures at 37 ℃. The resulting strain was designated Escherichia coli MG 1655. delta. pykF.

Preparation of variants of Escherichia coli having pykA impaired

PCR was carried out using pKD4 as a template and the oligo DNAs of SEQ ID Nos. 235 and 236 as primers to obtain a pykA-compromised PCR fragment of 1.6kb in sequence length.

The Escherichia coli MG 1655. delta. pykF strain was impaired in pykA by the same method as that for the preparation of the pykF impaired strain. The strain was introduced with pKD46 and then with a pykA-impairing PCR fragment. Colony direct PCR was performed using the obtained kanamycin-resistant strain, and the damage of the target gene and the insertion of the kanamycin-resistant gene were confirmed from the band length. The primers used were the oligo DNAs of SEQ ID Nos. 224 and 238.

Subsequently, pKD46 was released to obtain an ampicillin-sensitive strain. An ampicillin-resistant strain was again obtained by introducing pCP20 into the ampicillin-sensitive strain. The obtained strain was subjected to colony direct PCR, and the shedding of the kanamycin resistance gene was confirmed from the band length. The primers used were the oligo DNAs of SEQ ID Nos. 237 and 238. pCP20 was detached from the kanamycin-sensitive strain. The obtained strain was designated as Ec. DELTA.PP.

Example 5

Preparation of Escherichia coli variant having plasmid introduced thereinto which impairs pyruvate kinase function and expresses PEP carboxylase and enzyme catalyzing reactions A, B, E and F

The plasmids prepared in reference example 1 were introduced into Ec Δ PP prepared in example 4, respectively, to prepare Escherichia coli variants.

EcΔ PP was inoculated into 5mL of LB medium and shake-cultured at 30 ℃ for 1 day. 0.5mL of the culture broth was inoculated into 5mL of LB medium and shake-cultured at 30 ℃ for 2 hours. After the culture solution was cooled in ice for 20 minutes, the cells were washed 3 times with 10% (w/w) glycerol. The washed pellet was suspended in 100. mu.L of 10% (w/w) glycerol, and mixed with 1. mu.L of pBBR1MCS-2:: ATCTRO 1PCK, pBBR1MCS-2:: ATCTRO 2PCK, pBBR1MCS-2:: ATCTRO 3PCK, pBBR1MCS-2:: ATCTRO 4PCK, pBBR1MCS-2:: ATCTRO 5PCK, pBBR1MCS-2:: ATCTRO 6PCK or pBBR1MCS-2:: ATCTRO 7PCK, followed by ice-cooling in an electroporation cuvette for 10 minutes. After electroporation (3kV, 200. omega. and 25. mu.F) was performed using Gene pulser (Bio-rad), 1mL of SOC medium was added immediately, and shaking culture was performed at 30 ℃ for 1 hour. 50 μ L was spread on LB agar medium containing 25 μ g/mL kanamycin, and incubated at 30 ℃ for 1 day. The obtained strains were designated as Ec Δ PP/3HA1PCK, Ec Δ PP/3HA2PCK, Ec Δ PP/3HA3PCK, Ec Δ PP/3HA4PCK, Ec Δ PP/3HA5PCK, Ec Δ PP/3HA6PCK, and Ec Δ PP/3HA7PCK, respectively.

Reference example 9

Preparation of Escherichia coli variant having plasmid introduced thereinto which expresses PEP carboxylase and enzyme catalyzing reactions A, B, E and F without impairing pyruvate kinase function

In the same manner as in example 5, pBBR1MCS-2:, ATCTOR1PCK, pBBR1MCS-2:, ATCTOR2PCK, pBBR1MCS-2:, ATCTOR3PCK, pBBR1MCS-2:, ATCTOR4PCK, pBBR1MCS-2:, ATCTOR5PCK, pBBR1MCS-2:, ATCTOR6PCK or pBBR1MCS-2:, ATCTOR7PCK was introduced into Escherichia coli MG 1655. In addition, as a control, a strain in which pBBR1MCS-2 was introduced as a blank vector into Escherichia coli MG1655 was prepared. The resulting strains were designated as Ec/3HA1PCK, Ec/3HA2PCK, Ec/3HA3PCK, Ec/3HA4PCK, Ec/3HA5PCK, Ec/3HA6PCK, Ec/3HA7PCK, and Ec/pBBR (negative control), respectively.

Reference example 10

Preparation of Escherichia coli variant having plasmid introduced thereinto which impairs pyruvate kinase function and expresses enzyme catalyzing reactions A, B, E and F

In the same manner as in example 5, pBBR1MCS-2:, ATCTRR 1, pBBR1MCS-2:, ATCTRR 2, pBBR1MCS-2:, ATCTRR 3, pBBR1MCS-2:, ATCTRR 4, pBBR1MCS-2:, ATCTRR 5, pBBR1MCS-2:, ATCTRR 6 or pBBR1MCS-2:, ATCTRR 7 was introduced into Ec Δ PP. In addition, as a control, a strain in which pBBR1MCS-2 was introduced as a blank vector into Ec Δ PP was prepared. The resulting strains were designated as Ec Δ PP/3HA1, Ec Δ PP/3HA2, Ec Δ PP/3HA3, Ec Δ PP/3HA4, Ec Δ PP/3HA5, Ec Δ PP/3HA6, Ec Δ PP/3HA7, and Ec Δ PP/pBBR (negative control), respectively.

Example 6

Test for producing 3-hydroxyadipic acid and alpha-hydrohexadiene diacid using a variant of Escherichia coli having impaired pyruvate kinase function and enhanced PEP carboxylase kinase activity

The mutants prepared in example 5 were cultured in the same manner as in example 3. The concentrations of 3-hydroxyadipic acid, α -hydrogenated adipic acid, and other products accumulated in the culture supernatant and of the sugars remaining unused in the medium were quantified. The yields of 3-hydroxyadipic acid and α -hydrogenated adipic acid calculated from these values by using the above formula (2) are shown in Table 6.

Comparative example 3

Assays for the production of 3-hydroxyadipic acid and alpha-hydrohexadiene diacid using variants of Escherichia coli that do not compromise pyruvate kinase function and that have enhanced PEP carboxylase kinase activity

The mutants prepared in reference example 9 were cultured in the same manner as in example 3. The concentrations of 3-hydroxyadipic acid, α -hydrogenated adipic acid, and other products accumulated in the culture supernatant and of the sugars remaining unused in the medium were quantified. The yields of 3-hydroxyadipic acid and α -hydrogenated adipic acid calculated from these values by using the above formula (2) are shown in Table 6.

Comparative example 4

Assays for the production of 3-hydroxyadipic acid and alpha-hydrohexadiene diacid using variants of Escherichia coli with impaired pyruvate kinase function and without potentiating PEP carboxylase kinase activity

The mutants prepared in reference example 10 were cultured in the same manner as in example 3. The concentrations of 3-hydroxyadipic acid, α -hydrogenated adipic acid, and other products accumulated in the culture supernatant and of the sugars remaining unused in the medium were quantified. Further, the yields of 3-hydroxyadipic acid and α -hydrogenated adipic acid calculated from the results using the above formula (2) are shown in table 6.

When the results of comparative examples 3 and 4 are compared with the results of example 6, it is understood that: by impairing the function of pyruvate kinase of Escherichia coli and enhancing the activity of PEP carboxylase, the yields of 3-hydroxyadipic acid and alpha-hydrohexadiene diacid can be increased.

[ Table 6]

Example 7

Preparation of a variant of a microorganism belonging to the genus Serratia into which a plasmid is introduced, which impairs the function of pyruvate kinase and expresses PEP carboxylase and an enzyme catalyzing reactions A, B, C, E and F

The plasmid pMW119:: EH prepared in reference example 2 was introduced into each of the Serratia microorganism variants prepared in example 2, to prepare Serratia microorganism variants.

Sg.DELTA.PP/3 HA1PCK, Sg.DELTA.PP/3 HA2PCK, Sg.DELTA.PP/3 HA3PCK, Sg.DELTA.PP/3 HA4PCK, Sg.DELTA.PP/3 HA5PCK, Sg.DELTA.PP/3 HA6PCK, and Sg.DELTA.PP/3 HA7PCK were inoculated into 5mL of LB medium containing 25. mu.g/mL of kanamycin, and shake-cultured at 30 ℃ for 1 day. 0.5mL of the culture broth was inoculated into 5mL of LB medium containing 25. mu.g/mL of kanamycin, and shake-cultured at 30 ℃ for 2 hours. After the culture solution was cooled in ice for 20 minutes, the cells were washed 3 times with 10% (w/w) glycerol. The washed pellet was suspended in 100. mu.L of 10% (w/w) glycerol, mixed with 1. mu.L of pMW119:: EH, and then cooled in an electroporation cuvette for 10 minutes with ice. After electroporation (3kV, 200. omega. and 25. mu.F) was performed using Gene pulser (Bio-rad), 1mL of SOC medium was added immediately, and shaking culture was performed at 30 ℃ for 1 hour. 50. mu.L of the suspension was spread on LB agar medium containing 500. mu.g/mL ampicillin and 25. mu.g/mL kanamycin, and incubated at 30 ℃ for 1 day. The obtained strains were designated as Sg. delta. PP/HMA1PCK, Sg. delta. PP/HMA2PCK, Sg. delta. PP/HMA3PCK, Sg. delta. PP/HMA4PCK, Sg. delta. PP/HMA5PCK, Sg. delta. PP/HMA6PCK, and Sg. delta. PP/HMA7PCK, respectively.

Reference example 11

Preparation of a variant of a microorganism belonging to the genus Serratia into which a plasmid expressing PEP carboxylase and an enzyme catalyzing reactions A, B, C, E and F was introduced without impairing the function of pyruvate kinase

pMW119: EH was introduced into Sg/3HA1PCK, Sg/3HA2PCK, Sg/3HA3PCK, Sg/3HA4PCK, Sg/3HA5PCK, Sg/3HA6PCK, Sg/3HA7PCK by the same method as in example 7. In addition, as a control, a strain in which pMW119 was introduced as a blank vector into Sg/pBBR was prepared. The resulting strains were designated as Sg/HMA1PCK, Sg/HMA2PCK, Sg/HMA3PCK, Sg/HMA4PCK, Sg/HMA5PCK, Sg/HMA6PCK, Sg/HMA7PCK, and Sg/pBBRpMW, respectively (negative control).

Reference example 12

Preparation of a variant of a microorganism belonging to the genus Serratia into which a plasmid is introduced, which impairs the function of pyruvate kinase and expresses an enzyme that catalyzes reactions A, B, C, E and F

In the same manner as in example 7, pMW119: EH was introduced into Sg. DELTA. PP/3HA1, Sg. DELTA. PP/3HA2, Sg. DELTA. PP/3HA3, Sg. DELTA. PP/3HA4, Sg. DELTA. PP/3HA5, Sg. DELTA. PP/3HA6, and Sg. DELTA. PP/3HA 7. In addition, as a control, a strain in which pMW119 was introduced as a blank vector into Sg. DELTA.PP/pBBR was prepared. The obtained strains were designated as Sg. DELTA. PP/HMA1, Sg. DELTA. PP/HMA2, Sg. DELTA. PP/HMA3, Sg. DELTA. PP/HMA4, Sg. DELTA. PP/HMA5, Sg. DELTA. PP/HMA6, Sg. DELTA. PP/HMA7, and Sg. DELTA. PP/pBBRpMW, respectively (negative control).

Example 8

Test for producing alpha-hydrogenated adipic acid Using variants of microorganisms of the genus Serratia in which the function of pyruvate kinase is impaired and the Activity of PEP carboxylase is enhanced

The mutant strain prepared in example 7 was cultured in the same manner as in example 3 except that ampicillin was added at an amount of 500. mu.g/mL to the culture medium. The concentrations of the alpha-hydrogenated adipic acid and other products accumulated in the culture supernatant and the saccharides remaining in the culture medium without being used were quantified. The yield of α -hydrogenated adipic acid calculated from this value by using the above formula (2) is shown in Table 7.

Comparative example 5

Test for producing alpha-hydrogenated adipic acid Using variants of microorganisms of the genus Serratia which do not impair the function of pyruvate kinase and in which the Activity of PEP carboxylase kinase is enhanced

The mutants prepared in reference example 11 were cultured in the same manner as in example 8. The concentrations of the alpha-hydrogenated adipic acid and other products accumulated in the culture supernatant and the saccharides remaining in the culture medium without being used were quantified. The yield of α -hydrogenated adipic acid calculated from this value by using the above formula (2) is shown in Table 7.

Comparative example 6

Test for producing alpha-hydrogenated adipic acid Using variants of microorganisms of the genus Serratia in which the function of pyruvate kinase is impaired and the Activity of PEP carboxylase is not enhanced

The mutants prepared in reference example 12 were cultured in the same manner as in example 8. The concentrations of the alpha-hydrogenated adipic acid and other products accumulated in the culture supernatant and the saccharides remaining in the culture medium without being used were quantified. Further, the yield of α -hydrogenated adipic acid calculated from the results by using the above formula (2) is shown in table 7.

When the results of comparative examples 5 and 6 were compared with the results of example 8, it was found that: by damaging the pyruvate kinase gene of a microorganism belonging to the genus Serratia and enhancing the activity of PEP carboxylase, the yield of alpha-hydrogenated adipic acid can be increased.

[ Table 7]

Example 9

Preparation of Escherichia coli variant having plasmid introduced thereinto which impairs pyruvate kinase function and expresses PEP carboxylase and enzyme catalyzing reactions A, B, C, E and F

The Escherichia coli variants prepared in example 5 were introduced into the plasmid pMW119 prepared in reference example 2, EH, to prepare Escherichia coli variants.

EcDELTA PP/3HA1PCK, EcDELTA PP/3HA2PCK, EcDELTA PP/3HA3PCK, EcDELTA PP/3HA4PCK, EcDELTA PP/3HA5PCK, EcDELTA PP/3HA6PCK, and EcDELTA PP/3HA7PCK were inoculated into 5mL of LB medium containing 25. mu.g/mL of kanamycin, and shake-cultured at 30 ℃ for 1 day. 0.5mL of the culture broth was inoculated into 5mL of LB medium containing 25. mu.g/mL of kanamycin, and shake-cultured at 30 ℃ for 2 hours. After the culture solution was cooled in ice for 20 minutes, the cells were washed 3 times with 10% (w/w) glycerol. The washed pellet was suspended in 100. mu.L of 10% (w/w) glycerol, mixed with 1. mu.L of pMW119:: EH, and then cooled in an electroporation cuvette for 10 minutes with ice. After electroporation (3kV, 200. omega. and 25. mu.F) was performed using Gene pulser (Bio-rad), 1mL of SOC medium was added immediately, and shaking culture was performed at 30 ℃ for 1 hour. 50 μ L of the suspension was spread on LB agar medium containing 100 μ g/mL ampicillin and 25 μ g/mL kanamycin, and incubated at 30 ℃ for 1 day. The obtained strains were designated as Ec Δ PP/HMA1PCK, Ec Δ PP/HMA2PCK, Ec Δ PP/HMA3PCK, Ec Δ PP/HMA4PCK, Ec Δ PP/HMA5PCK, Ec Δ PP/HMA6PCK, and Ec Δ PP/HMA7PCK, respectively.

Reference example 13

Preparation of Escherichia coli variant having plasmid introduced thereinto which expresses PEP carboxylase and enzyme catalyzing reactions A, B, C, E and F without impairing pyruvate kinase function

pMW119: EH was introduced into Ec/3HA1PCK, Ec/3HA2PCK, Ec/3HA3PCK, Ec/3HA4PCK, Ec/3HA5PCK, Ec/3HA6PCK, Ec/3HA7PCK by the same method as in example 9. In addition, as a control, a strain in which pMW119 was introduced as a blank vector into Ec/pBBR was prepared. The resulting strains were designated Ec/HMA1PCK, Ec/HMA2PCK, Ec/HMA3PCK, Ec/HMA4PCK, Ec/HMA5PCK, Ec/HMA6PCK, Ec/HMA7PCK, Ec/pBBRpMW, respectively (negative control).

Reference example 14

Preparation of Escherichia coli variant having plasmid introduced thereinto which impairs pyruvate kinase function and expresses enzyme catalyzing reactions A, B, C, E and F

In the same manner as in example 9, pMW119:, Ec. DELTA.PP/3 HA1, Ec. DELTA.PP/3 HA2, Ec. DELTA.PP/3 HA3, Ec. DELTA.PP/3 HA4, Ec. DELTA.PP/3 HA5, Ec. DELTA.PP/3 HA6, and Ec. DELTA.PP/3 HA7 were introduced. In addition, as a control, a strain in which pMW119 was introduced as a blank vector into Ec Δ PP/pBBR was prepared. The obtained strains were designated as Ec Δ PP/HMA1, Ec Δ PP/HMA2, Ec Δ PP/HMA3, Ec Δ PP/HMA4, Ec Δ PP/HMA5, Ec Δ PP/HMA6, Ec Δ PP/HMA7, and Ec Δ PP/pBBRpMW (negative control), respectively.

Example 10

Test for producing alpha-hydrogenated adipic acid Using variants of Escherichia coli having impaired pyruvate kinase function and enhanced PEP carboxylase Activity

The mutant strain prepared in example 9 was cultured in the same manner as in example 6 except that ampicillin was added at a concentration of 100. mu.g/mL to the culture medium. The concentrations of the alpha-hydrogenated adipic acid and other products accumulated in the culture supernatant and the saccharides remaining in the culture medium without being used were quantified. The yield of α -hydrogenated adipic acid calculated from this value by using the above formula (2) is shown in Table 8.

Comparative example 7

Test for producing alpha-hydrogenated adipic acid Using variants of Escherichia coli which do not impair the function of pyruvate kinase and in which the Activity of PEP-carboxylated kinase is enhanced

The mutants prepared in reference example 13 were cultured in the same manner as in example 10. The concentrations of the alpha-hydrogenated adipic acid and other products accumulated in the culture supernatant and the saccharides remaining in the culture medium without being used were quantified. The yield of α -hydrogenated adipic acid calculated from this value by using the above formula (2) is shown in Table 8.

Comparative example 8

Test for producing alpha-hydrogenated adipic acid Using variants of Escherichia coli in which the function of pyruvate kinase is impaired and the Activity of PEP-carboxylated kinase is not enhanced

The mutants prepared in reference example 14 were cultured in the same manner as in example 10. The concentrations of the alpha-hydrogenated adipic acid and other products accumulated in the culture supernatant and the saccharides remaining in the culture medium without being used were quantified. Further, the yield of α -hydrogenated adipic acid calculated from the results by using the above formula (2) is shown in table 8.

When the results of comparative examples 7 and 8 were compared with the results of example 10, it was found that: by impairing the function of pyruvate kinase of Escherichia coli and enhancing the activity of PEP carboxylation kinase, the yield of alpha-hydrogenated adipic acid can be increased.

[ Table 8]

Example 11

Preparation of a variant of a microorganism belonging to the genus Serratia into which a plasmid is introduced, which impairs the function of pyruvate kinase and expresses PEP carboxylase and an enzyme catalyzing reactions A, B, C, D and G

In the same manner as in example 2, pBBR1MCS-2: (ATCT 2OR1 PCK), pBBR1MCS-2: (ATCT 2OR2 PCK), pBBR1MCS-2: (ATCT 2OR3 PCK), pBBR1MCS-2: (ATCT 2OR4 PCK), pBBR 2 MCS-2: (ATCT 2OR5 PCK), pBBR1MCS-2: (ATCT 2OR6 PCK), OR pBBR1MCS-2: (ATCT 2OR7 PCK) prepared in reference example 6 was introduced into Sg. DELTA.PP. The plasmid pMW119:: EHER prepared in reference example 4 was introduced into each of the obtained variants in the same manner as in example 7 to prepare a microorganism variant of Serratia. The obtained strains were designated as Sg. delta. PP/ADA1PCK, Sg. delta. PP/ADA2PCK, Sg. delta. PP/ADA3PCK, Sg. delta. PP/ADA4PCK, Sg. delta. PP/ADA5PCK, Sg. delta. PP/ADA6PCK, and Sg. delta. PP/ADA7PCK, respectively.

Reference example 15

Preparation of a variant of a microorganism belonging to the genus Serratia into which a plasmid expressing PEP carboxylase and an enzyme catalyzing reactions A, B, C, D and G was introduced without impairing the function of pyruvate kinase

In the same manner as in example 2, pBBR1MCS-2: (ATCT 2OR1 PCK), pBBR1MCS-2: (ATCT 2OR2 PCK), pBBR1MCS-2: (ATCT 2OR3 PCK), pBBR1MCS-2: (ATCT 2OR4 PCK), pBBR1MCS-2: (ATCT 2OR5 PCK), pBBR1MCS-2: (ATCT 2OR6 PCK), OR pBBR1MCS-2: (ATCT 2OR7 PCK) prepared in reference example 6 was introduced into Serratia grimeii NBRC 13537. The plasmid pMW119:: EHER prepared in reference example 4 was introduced into each of the obtained variants in the same manner as in example 7 to prepare a microorganism variant of Serratia. The obtained strains were designated as Sg/ADA1PCK, Sg/ADA2PCK, Sg/ADA3PCK, Sg/ADA4PCK, Sg/ADA5PCK, Sg/ADA6PCK, and Sg/ADA7PCK, respectively.

Reference example 16

Preparation of a variant of a microorganism belonging to the genus Serratia into which a plasmid is introduced, which impairs the function of pyruvate kinase and expresses an enzyme that catalyzes reactions A, B, C, D and G

In the same manner as in example 2, pBBR1 MCS-2:ATCT2 OR1, pBBR1 MCS-2:ATCT2 OR2, pBBR1 MCS-2:ATCT2 OR3, pBBR1 MCS-2:ATCT2 OR4, pBBR1 MCS-2:ATCT2 OR5, pBBR1 MCS-2:ATCT2 OR6, OR pBBR1 MCS-2:ATCT2 OR7 were introduced into Sg.DELTA.PP. The plasmid pMW119:: EHER prepared in reference example 4 was introduced into each of the obtained variants in the same manner as in example 7 to prepare a microorganism variant of Serratia. The obtained strains were designated as Sg. DELTA. PP/ADA1, Sg. DELTA. PP/ADA2, Sg. DELTA. PP/ADA3, Sg. DELTA. PP/ADA4, Sg. DELTA. PP/ADA5, Sg. DELTA. PP/ADA6, and Sg. DELTA. PP/ADA7, respectively.

Example 12

Test for producing adipic acid Using variants of microorganisms of the genus Serratia in which the function of pyruvate kinase is impaired and the Activity of PEP carboxylase is enhanced

The mutant strain prepared in example 11 was cultured in the same manner as in example 3 except that ampicillin was added at an amount of 500. mu.g/mL to the culture medium. The concentrations of adipic acid and other products accumulated in the culture supernatant and of the residual sugars not used in the culture medium were quantified. The adipic acid was quantified by using LC-MS/MS under the same conditions as those of 3-hydroxyadipic acid and α -hydrogenated adipic acid. The yield of adipic acid calculated from this value by using the above formula (2) is shown in Table 9.

Comparative example 9

Test for producing adipic acid Using variants of microorganisms of the genus Serratia which do not impair the function of pyruvate kinase and in which the Activity of PEP carboxylase is enhanced

The mutants prepared in reference example 15 and Sg/pBBRpMW were cultured in the same manner as in example 12. The concentrations of adipic acid and other products accumulated in the culture supernatant and of the residual sugars not used in the culture medium were quantified. The yield of adipic acid calculated from this value by using the above formula (2) is shown in Table 9.

Comparative example 10

Assay for adipic acid production Using variants of microorganisms of the genus Serratia that impaired pyruvate kinase function and did not potentiate PEP carboxylase Activity

The mutants prepared in reference example 16 and Sg.DELTA.PP/pBBRpMW were cultured in the same manner as in example 12. The concentrations of adipic acid and other products accumulated in the culture supernatant and of the residual sugars not used in the culture medium were quantified. Further, the yield of adipic acid calculated from the results by using the above formula (2) is shown in table 9.

When the results of comparative examples 9 and 10 were compared with the results of example 12, it was found that: by impairing the function of pyruvate kinase of a microorganism belonging to the genus Serratia and enhancing the activity of PEP carboxylation kinase, the yield of adipic acid can be increased.

[ Table 9]

Example 13

Preparation of Escherichia coli variant having plasmid introduced thereinto which impairs pyruvate kinase function and expresses PEP carboxylase and enzyme catalyzing reactions A, B, C, D and G

The EcDeltaPP was introduced with pBBR1MCS-2: (ATCT 2OR1 PCK), pBBR1MCS-2: (ATCT 2OR2 PCK), pBBR1MCS-2: (ATCT 2OR3 PCK), pBBR1MCS-2: (ATCT 2OR4 PCK), pBBR 2 MCS-2: (ATCT 2OR5 PCK), pBBR1MCS-2: (ATCT 2OR6 PCK), OR pBBR1MCS-2: (ATCT 2OR7 PCK) prepared in reference example 6, by the same method as in example 5. The plasmid pMW119:: EHER prepared in reference example 4 was introduced into each of the obtained variants in the same manner as in example 9 to prepare a variant of Escherichia coli. The obtained strains were designated as Ec Δ PP/ADA1PCK, Ec Δ PP/ADA2PCK, Ec Δ PP/ADA3PCK, Ec Δ PP/ADA4PCK, Ec Δ PP/ADA5PCK, Ec Δ PP/ADA6PCK, and Ec Δ PP/ADA7PCK, respectively.

Reference example 17

Preparation of Escherichia coli variant having plasmid introduced thereinto which expresses PEP carboxylase and enzyme catalyzing reactions A, B, C, D and G without impairing pyruvate kinase function

The Escherichia coli MG1655 was introduced with the pBBR1MCS-2:, ATCT2OR1PCK, pBBR1MCS-2:, ATCT2OR2PCK, pBBR1MCS-2:, ATCT2OR3PCK, pBBR1MCS-2:, ATCT2OR4PCK, pBBR1MCS-2:, ATCT2OR5PCK, pBBR1MCS-2:, ATCT2OR6PCK, OR pBBR 1-2:, ATCT2OR7PCK, which were prepared in reference example 6, in the same manner as in example 5. The plasmid pMW119:, EHER, prepared in reference example 4 was introduced into each of the obtained mutants by the same method as in example 9 to prepare mutants. The resulting strains were designated Ec/ADA1PCK, Ec/ADA2PCK, Ec/ADA3PCK, Ec/ADA4PCK, Ec/ADA5PCK, Ec/ADA6PCK, and Ec/ADA7PCK, respectively.

Reference example 18

Preparation of Escherichia coli variant having plasmid introduced thereinto which impairs pyruvate kinase function and expresses enzyme catalyzing reactions A, B, C, D and G

The EcDeltaPP was introduced with pBBR1 MCS-2:ATCT2 OR1, pBBR1 MCS-2:ATCT2 OR2, pBBR1 MCS-2:ATCT2 OR3, pBBR1 MCS-2:ATCT2 OR4, pBBR1 MCS-2:ATCT2 OR5, pBBR1 MCS-2:ATCT2 OR6, OR pBBR1 MCS-2:ATCT2 OR7 in the same manner as in example 5. The plasmid pMW119:, EHER, prepared in reference example 4 was introduced into each of the obtained mutants by the same method as in example 9 to prepare mutants. The obtained strains were designated as Ec Δ PP/ADA1, Ec Δ PP/ADA2, Ec Δ PP/ADA3, Ec Δ PP/ADA4, Ec Δ PP/ADA5, Ec Δ PP/ADA6, and Ec Δ PP/ADA7, respectively.

Example 14

Test for producing adipic acid Using variants of Escherichia coli having impaired function of pyruvate kinase and enhanced Activity of PEP carboxylase

The mutant strain prepared in example 13 was cultured in the same manner as in example 6 except that ampicillin was added at 500. mu.g/mL to the culture medium. The concentrations of adipic acid and other products accumulated in the culture supernatant and of the residual sugars not used in the culture medium were quantified. The adipic acid was quantified by using LC-MS/MS under the same conditions as those of 3-hydroxyadipic acid and α -hydrogenated adipic acid. The yield of adipic acid calculated from this value by using the above formula (2) is shown in Table 10.

Comparative example 11

Test for producing adipic acid Using variants of Escherichia coli which do not impair the function of pyruvate kinase and in which the Activity of PEP-carboxylated kinase is enhanced

The mutants prepared in reference example 17 were cultured in the same manner as in example 14. The concentrations of adipic acid and other products accumulated in the culture supernatant and of the residual sugars not used in the culture medium were quantified. The yield of adipic acid calculated from this value by using the above formula (2) is shown in Table 10.

Comparative example 12

Assay for the production of adipic acid Using variants of Escherichia coli which have impaired pyruvate kinase function and which have not enhanced PEP carboxylase kinase Activity

The mutants prepared in reference example 18 were cultured in the same manner as in example 14. The concentrations of adipic acid and other products accumulated in the culture supernatant and of the residual sugars not used in the culture medium were quantified. Further, the yield of adipic acid calculated from the results by using the above formula (2) is shown in table 10.

When the results of comparative examples 11 and 12 were compared with the results of example 14, it was found that: by impairing the function of pyruvate kinase of Escherichia coli and enhancing the activity of PEP carboxylation kinase, the yield of adipic acid can be increased.

[ Table 10]

Example 15

Test 2 for production of 3-hydroxyadipic acid and α -hydrohexadiene diacid Using a variant of a microorganism belonging to the genus Serratia, in which the function of pyruvate kinase is impaired and the activity of PEP carboxylase is enhanced

Production tests of 3-hydroxyadipic acid and α -hydrohexadiene diacid were performed under anaerobic conditions using the microorganism variants of Serratia prepared in example 2.

The microorganism variant of Serratia prepared in example 2 was cultured in the same manner as in example 3, except that the culture was performed using the medium II under standing. The concentrations of 3-hydroxyadipic acid, α -hydrogenated adipic acid, and other products accumulated in the culture supernatant and of the sugars remaining unused in the medium were quantified. The yields of 3-hydroxyadipic acid and α -hydrogenated adipic acid calculated from these values by using the above formula (2) are shown in Table 11.

Comparative example 13

Test 2 for production of 3-hydroxyadipic acid and α -hydrohexadiene diacid Using a variant of a microorganism belonging to the genus Serratia which does not impair the function of pyruvate kinase and enhances the activity of PEP carboxylase

The mutants prepared in reference example 7 were cultured in the same manner as in example 15. The concentrations of 3-hydroxyadipic acid, α -hydrogenated adipic acid, and other products accumulated in the culture supernatant and of the sugars remaining unused in the medium were quantified. The yields of 3-hydroxyadipic acid and α -hydrogenated adipic acid calculated from these values by using the above formula (2) are shown in Table 11.

Comparative example 14

Test 2 for production of 3-hydroxyadipic acid and α -hydrohexadiene diacid Using a variant of a microorganism belonging to the genus Serratia, in which the function of pyruvate kinase was impaired and the activity of PEP-carboxylated kinase was not enhanced

The mutants prepared in reference example 8 were cultured in the same manner as in example 15. The concentrations of 3-hydroxyadipic acid, α -hydrogenated adipic acid, and other products accumulated in the culture supernatant and of the sugars remaining unused in the medium were quantified. The yields of 3-hydroxyadipic acid and α -hydrogenated adipic acid calculated from these values by using the above formula (2) are shown in Table 11.

When the results of comparative examples 13 and 14 were compared with the results of example 15, it was found that: by impairing the function of pyruvate kinase of a microorganism belonging to the genus Serratia and enhancing the activity of PEP carboxylase, the yields of 3-hydroxyadipic acid and a-hydrohexadiene diacid can be improved even under anaerobic conditions.

[ Table 11]

Example 16

Test 2 for production of 3-hydroxyadipic acid and alpha-hydrohexadiene diacid Using variants of Escherichia coli having impaired function of pyruvate kinase

Production tests of 3-hydroxyadipic acid and α -hydrogenated adipic acid were carried out under anaerobic conditions using the Escherichia coli variants prepared in example 5.

The Escherichia coli variant prepared in example 5 was cultured in the same manner as in example 6, except that the culture was performed in the presence of the medium II. The concentrations of 3-hydroxyadipic acid, α -hydrogenated adipic acid, and other products accumulated in the culture supernatant and of the sugars remaining unused in the medium were quantified. The yields of 3-hydroxyadipic acid and α -hydrogenated adipic acid calculated from these values by using the above formula (2) are shown in Table 12.

Comparative example 15

Test 2 for production of 3-hydroxyadipic acid and α -hydrohexadiene diacid Using a variant of Escherichia coli which does not impair the function of pyruvate kinase and which has enhanced the Activity of PEP-carboxylated kinase

The mutants prepared in reference example 9 were cultured in the same manner as in example 16. The concentrations of 3-hydroxyadipic acid, α -hydrogenated adipic acid, and other products accumulated in the culture supernatant and of the sugars remaining unused in the medium were quantified. The yields of 3-hydroxyadipic acid and α -hydrogenated adipic acid calculated from these values by using the above formula (2) are shown in Table 12.

Comparative example 16

Test 2 for production of 3-hydroxyadipic acid and α -hydrohexadiene diacid Using variants of Escherichia coli in which the function of pyruvate kinase was impaired and the Activity of PEP-carboxylated kinase was not enhanced

The mutants prepared in reference example 10 were cultured in the same manner as in example 16. The concentrations of 3-hydroxyadipic acid, α -hydrogenated adipic acid, and other products accumulated in the culture supernatant and of the sugars remaining unused in the medium were quantified. The yields of 3-hydroxyadipic acid and α -hydrogenated adipic acid calculated from these values by using the above formula (2) are shown in Table 12.

When the results of comparative examples 15 and 16 were compared with the results of example 16, it was found that: by impairing the function of pyruvate kinase of Escherichia coli and enhancing the activity of PEP carboxylase, the yields of 3-hydroxyadipic acid and a-hydrohexadiene diacid can be increased even under anaerobic conditions.

[ Table 12]

Example 17

Experiment 2 for producing adipic acid by using a variant of a microorganism belonging to the genus Serratia, in which the function of pyruvate kinase is impaired and the activity of PEP carboxylase is enhanced

Using the serratia microorganism variant prepared in example 11, an adipic acid production test was performed under anaerobic conditions.

The microorganism variant of Serratia produced in example 11 was cultured in the same manner as in example 12, except that the culture was performed using the medium II under standing. The concentrations of adipic acid and other products accumulated in the culture supernatant and of the residual sugars not used in the culture medium were quantified. The yield of adipic acid calculated from this value by using the above formula (2) is shown in Table 13.

Comparative example 17

Test 2 for producing adipic acid Using a variant of a microorganism belonging to the genus Serratia which does not impair the function of pyruvate kinase and enhances the activity of PEP carboxylase kinase

The mutants prepared in reference example 15 were cultured in the same manner as in example 17. The concentrations of adipic acid and other products accumulated in the culture supernatant and of the residual sugars not used in the culture medium were quantified. The yield of adipic acid calculated from this value by using the above formula (2) is shown in Table 13.

Comparative example 18

Experiment 2 for producing adipic acid by using a variant of a microorganism belonging to the genus Serratia, in which the function of pyruvate kinase is impaired and the activity of PEP carboxylase kinase is not enhanced

The mutants prepared in reference example 16 were cultured in the same manner as in example 17. The concentrations of adipic acid and other products accumulated in the culture supernatant and of the residual sugars not used in the culture medium were quantified. The yield of adipic acid calculated from this value by using the above formula (2) is shown in Table 13.

When the results of comparative examples 17 and 18 were compared with the results of example 17, it was found that: by impairing the function of pyruvate kinase of a microorganism belonging to the genus Serratia and enhancing the activity of PEP carboxylase, the yield of adipic acid can be increased even under anaerobic conditions.

[ Table 13]

Example 18

Test 2 for production of adipic acid Using variants of Escherichia coli having impaired function of pyruvate kinase

Using the escherichia coli variant prepared in example 13, an adipic acid production test was performed under anaerobic conditions. The Escherichia coli variant prepared in example 13 was cultured in the same manner as in example 14, except that the culture was performed in the presence of the medium II. The concentrations of adipic acid and other products accumulated in the culture supernatant and of the residual sugars not used in the culture medium were quantified. The yield of adipic acid calculated from this value by using the above formula (2) is shown in Table 14.

Comparative example 19

Test 2 for production of adipic acid Using variants of Escherichia coli in which the function of pyruvate kinase was not impaired and the activity of PEP carboxylase was enhanced

The mutants prepared in reference example 17 were cultured in the same manner as in example 18. The concentrations of adipic acid and other products accumulated in the culture supernatant and of the residual sugars not used in the culture medium were quantified. The yield of adipic acid calculated from this value by using the above formula (2) is shown in Table 14.

Comparative example 20

Test 2 for production of adipic acid Using variants of Escherichia coli in which the function of pyruvate kinase was impaired and the Activity of PEP-carboxylated kinase was not enhanced

The mutants prepared in reference example 18 were cultured in the same manner as in example 18. The concentrations of adipic acid and other products accumulated in the culture supernatant and of the residual sugars not used in the culture medium were quantified. The yield of adipic acid calculated from this value by using the above formula (2) is shown in Table 14.

When the results of comparative examples 19 and 20 were compared with the results of example 18, it was found that: by impairing the function of pyruvate kinase of Escherichia coli and enhancing the activity of PEP carboxylation kinase, the yield of adipic acid can be improved even under anaerobic conditions.

[ Table 14]

Reference example 19

Preparation of a variant of a microorganism belonging to the genus Serratia into which a plasmid expressing an enzyme catalyzing reactions A, B, E and F was introduced without impairing the function of pyruvate kinase

In the same manner as in example 2, pBBR1MCS-2 (control), pBBR1MCS-2:: ATCTOR1, pBBR1MCS-2:: ATCTOR2, pBBR1MCS-2:: ATCTOR3, pBBR1MCS-2:: ATCTOR4, pBBR1MCS-2:: ATTOR 5, pBBR1MCS-2:: ATCTOR6 or pBBR1MCS-2:: ATCTOR7 were introduced into Serratia grimesii NBRC 13537. The resulting strains were designated as Sg/pBBR (negative control), Sg/3HA1, Sg/3HA2, Sg/3HA3, Sg/3HA4, Sg/3HA5, Sg/3HA6, and Sg/3HA7, respectively.

Comparative example 21

Assay for the production of 3-hydroxyadipic acid and alpha-hydrohexadiene diacid using variants of a microorganism of the genus Serratia which have not been impaired with pyruvate kinase

The microorganism variant of Serratia prepared in reference example 19 was cultured in the same manner as in example 3. The concentrations of 3-hydroxyadipic acid, α -hydrogenated adipic acid, and other products accumulated in the culture supernatant and of the sugars remaining unused in the medium were quantified. Further, the yields of 3-hydroxyadipic acid and α -hydrogenated adipic acid calculated from the results using the above formula (2) are shown in table 15.

When the results of comparative example 21 were compared with those of comparative example 1, it was found that: in the case of a microorganism belonging to the genus Serratia in which the function of pyruvate kinase is not impaired, the yield of 3-hydroxyadipic acid and α -hydrohexadiene diacid is reduced when the activity of PEP carboxylase is enhanced as compared with the case where the activity is not enhanced.

[ Table 15]

Reference example 20

Preparation of Escherichia coli variant having plasmid introduced thereinto which expresses enzyme catalyzing reactions A, B, E and F without impairing pyruvate kinase function

In the same manner as in example 5, pBBR1MCS-2 (control), pBBR1MCS-2:: ATCTOR1, pBBR1MCS-2:: ATCTOR2, pBBR1MCS-2:: ATCTOR3, pBBR1MCS-2:: ATCTOR4, pBBR1MCS-2:: ATCTOR5, pBBR1MCS-2:: ATCTOR6 or pBBR1MCS-2:: ATCTOR7 was introduced into Escherichia coli MG 1655. The resulting strains were designated as Ec/pBBR (negative control), Ec/3HA1, Ec/3HA2, Ec/3HA3, Ec/3HA4, Ec/3HA5, Ec/3HA6, and Ec/3HA7, respectively.

Comparative example 22

Assay for the production of 3-hydroxyadipic acid and alpha-hydrohexadiene diacid using variants of Escherichia coli which do not compromise the function of pyruvate kinase

The mutants prepared in reference example 20 were cultured in the same manner as in example 6. The concentrations of 3-hydroxyadipic acid, α -hydrogenated adipic acid, and other products accumulated in the culture supernatant and of the sugars remaining unused in the medium were quantified. The yields of 3-hydroxyadipic acid and α -hydrogenated adipic acid calculated from these values by using the above formula (2) are shown in Table 16.

When the results of comparative example 22 were compared with those of comparative example 3, it was found that: in the case of Escherichia coli in which the function of pyruvate kinase is not impaired, the yield of 3-hydroxyadipic acid and α -hydrohexadiene diacid is reduced in the case where the activity of PEP carboxylase is enhanced as compared with the case where the activity is not enhanced.

[ Table 16]

Reference example 21

Preparation of a variant of a microorganism belonging to the genus Serratia into which a plasmid expressing an enzyme catalyzing reactions A, B, C, E and F was introduced without impairing the function of pyruvate kinase

In the same manner as in example 7, pMW119 (control) or pMW119:, EH was introduced into Sg/pBBR, Sg/3HA1, Sg/3HA2, Sg/3HA3, Sg/3HA4, Sg/3HA5, Sg/3HA6 and Sg/3HA 7. The obtained strains were designated as Sg/pBBRpMW (negative control), Sg/HMA1, Sg/HMA2, Sg/HMA3, Sg/HMA4, Sg/HMA5, Sg/HMA6, and Sg/HMA7, respectively.

Comparative example 23

Assay for the production of alpha-hydrogenated adipic acid Using variants of a microorganism of the genus Serratia which do not compromise the function of pyruvate kinase

The mutants prepared in reference example 21 were cultured in the same manner as in example 8. The concentrations of the alpha-hydrogenated adipic acid and other products accumulated in the culture supernatant and the saccharides remaining in the culture medium without being used were quantified. The yield of α -hydrogenated adipic acid calculated from this value by using the above formula (2) is shown in Table 17.

When the results of comparative example 23 were compared with those of comparative example 5, it was found that: in the case of a microorganism belonging to the genus Serratia in which the function of pyruvate kinase is not impaired, the yield of alpha-hydrogenated adipic acid is reduced in the case where the activity of PEP carboxylase is enhanced as compared with the case where the activity is not enhanced.

[ Table 17]

Reference example 22

Preparation of Escherichia coli variant having plasmid introduced thereinto which does not impair the function of pyruvate kinase and which expresses enzyme catalyzing reactions A, B, C, E and F

In the same manner as in example 9, pMW119 (control) or pMW119:, EH was introduced into Ec/pBBR, Ec/3HA1, Ec/3HA2, Ec/3HA3, Ec/3HA4, Ec/3HA5, Ec/3HA6 and Ec/3HA 7. The resulting strains were designated as Ec/pBBRpMW (negative control), Ec/HMA1, Ec/HMA2, Ec/HMA3, Ec/HMA4, Ec/HMA5, Ec/HMA6, and Ec/HMA7, respectively.

Comparative example 24

Assay for the production of alpha-hydrogenated adipic acid using variants of Escherichia coli which do not impair the function of pyruvate kinase

The mutants prepared in reference example 22 were cultured in the same manner as in example 10. The concentrations of the alpha-hydrogenated adipic acid and other products accumulated in the culture supernatant and the saccharides remaining in the culture medium without being used were quantified. The yield of α -hydrogenated adipic acid calculated from this value by using the above formula (2) is shown in Table 18.

When the results of comparative example 24 were compared with those of comparative example 7, it was found that: in the case of Escherichia coli in which the function of pyruvate kinase is not impaired, the yield of alpha-hydrogenated adipic acid is reduced in the case where the activity of PEP carboxylase is enhanced as compared with the case where the activity is not enhanced.

[ Table 18]

Reference example 23

Preparation of a variant of a microorganism belonging to the genus Serratia into which a plasmid expressing an enzyme catalyzing reactions A, B, C, D and G was introduced without impairing the function of pyruvate kinase

In the same manner as in example 11, pBBR1 MCS-2:ATCT2 OR1, pBBR1 MCS-2:ATCT2 OR2, pBBR1 MCS-2:ATCT2 OR3, pBBR1 MCS-2:ATCT2 OR4, pBBR1 MCS-2:ATCT2 OR5, pBBR1 MCS-2:ATCT2 OR6, OR pBBR1 MCS-2:ATCT2 OR7, each prepared in reference example 3, were introduced into Serratia grimesii NBRC 13537. The plasmid pMW119:: EHER prepared in reference example 4 was introduced into each of the obtained variants in the same manner as in example 7 to prepare a microorganism variant of Serratia. The obtained strains were designated as Sg/ADA1, Sg/ADA2, Sg/ADA3, Sg/ADA4, Sg/ADA5, Sg/ADA6, and Sg/ADA7, respectively.

Comparative example 25

Test for producing adipic acid Using variants of a microorganism of the genus Serratia which do not compromise the function of pyruvate kinase

The mutants prepared in reference example 23 and Sg/pBBRpMW were cultured in the same manner as in example 8. The concentrations of adipic acid and other products accumulated in the culture supernatant and of the residual sugars not used in the culture medium were quantified. The yield of adipic acid calculated from this value by using the above formula (2) is shown in Table 19.

When the results of comparative example 25 were compared with those of comparative example 9, it was found that: in the case of a microorganism belonging to the genus Serratia in which the function of pyruvate kinase is not impaired, the yield of adipic acid is reduced in the case where the activity of PEP carboxylase is enhanced as compared with the case where the activity is not enhanced.

[ Table 19]

Reference example 24

Preparation of Escherichia coli variant having plasmid introduced thereinto which does not impair the function of pyruvate kinase and expresses enzyme catalyzing reactions A, B, C, D and G

The Escherichia coli MG1655 was introduced with the pBBR1MCS-2:, ATCT2OR1, pBBR1MCS-2:, ATCT2OR2, pBBR1MCS-2:, ATCT2OR3, pBBR1MCS-2:, ATCT2OR4, pBBR1MCS-2:, ATCT2OR5, pBBR1MCS-2:, ATCT2OR6, OR pBBR1MCS-2:, ATCT2OR7, which were prepared in reference example 3, in the same manner as in example 13. The plasmid pMW119:: EHER prepared in reference example 4 was introduced into each of the obtained variants in the same manner as in example 9 to prepare a variant of Escherichia coli. The resulting strains were designated as Ec/ADA1, Ec/ADA2, Ec/ADA3, Ec/ADA4, Ec/ADA5, Ec/ADA6, and Ec/ADA7, respectively.

Comparative example 26

Test for the production of adipic acid Using variants of Escherichia coli which do not impair the function of pyruvate kinase

The mutants prepared in reference example 24 and Ec/pBBRpMW were cultured in the same manner as in example 10. The concentrations of adipic acid and other products accumulated in the culture supernatant and of the residual sugars not used in the culture medium were quantified. The yield of adipic acid calculated from this value by using the above formula (2) is shown in Table 20.

When the results of comparative example 26 were compared with those of comparative example 11, it was found that: in the case of Escherichia coli in which the function of pyruvate kinase is not impaired, the yield of adipic acid is reduced in the case where the activity of PEP carboxylase is enhanced as compared with the case where the activity is not enhanced.

[ Table 20]

Comparative example 27

Test 2 for production of 3-hydroxyadipic acid and alpha-hydrohexadiene diacid Using a variant of a microorganism of the genus Serratia which does not impair the function of pyruvate kinase

The mutants prepared in reference example 19 were cultured in the same manner as in example 15. The concentrations of 3-hydroxyadipic acid, α -hydrogenated adipic acid, and other products accumulated in the culture supernatant and of the sugars remaining unused in the medium were quantified. The yields of 3-hydroxyadipic acid and α -hydrogenated adipic acid calculated from these values by using the above formula (2) are shown in Table 21.

When the results of comparative example 27 were compared with those of comparative example 13, it was found that: in the case of a microorganism belonging to the genus Serratia in which the function of pyruvate kinase is not impaired, the yield of 3-hydroxyadipic acid and α -hydrohexadiene diacid is reduced in the case where the activity of PEP carboxylase is enhanced as compared with the case where the activity is not enhanced, even under anaerobic conditions.

[ Table 21]

Comparative example 28

Test 2 for the production of 3-hydroxyadipic acid and alpha-hydrohexadiene diacid Using variants of Escherichia coli which do not impair the function of pyruvate kinase

The mutants prepared in reference example 20 were cultured in the same manner as in example 16. The concentrations of 3-hydroxyadipic acid, α -hydrogenated adipic acid, and other products accumulated in the culture supernatant and of the sugars remaining unused in the medium were quantified. The yields of 3-hydroxyadipic acid and α -hydrogenated adipic acid calculated from these values by using the above formula (2) are shown in Table 22.

When the results of comparative example 28 were compared with those of comparative example 15, it was found that: in the case of Escherichia coli in which the function of pyruvate kinase is not impaired, the yield of 3-hydroxyadipic acid and α -hydrohexadiene diacid is reduced in the case where the activity of PEP carboxylase is enhanced as compared with the case where the activity is not enhanced, even under anaerobic conditions.

[ Table 22]

Comparative example 29

Test 2 for production of adipic acid Using a variant of a microorganism of the genus Serratia which does not impair the function of pyruvate kinase

The mutants prepared in reference example 23 were cultured in the same manner as in example 17. The concentrations of adipic acid and other products accumulated in the culture supernatant and of the residual sugars not used in the culture medium were quantified. The yield of adipic acid calculated from the values by using the above formula (2) is shown in Table 23.

When the results of comparative example 29 were compared with those of comparative example 17, it was found that: in the case of a microorganism belonging to the genus Serratia in which the function of pyruvate kinase is not impaired, the yield of adipic acid is reduced in the case where the activity of PEP carboxylase is enhanced as compared with the case where the activity is not enhanced, even under anaerobic conditions.

[ Table 23]

Comparative example 30

Test 2 for production of adipic acid Using variants of Escherichia coli which do not impair the function of pyruvate kinase

The mutants prepared in reference example 24 were cultured in the same manner as in example 18. The concentrations of adipic acid and other products accumulated in the culture supernatant and of the residual sugars not used in the culture medium were quantified. The yield of adipic acid calculated from this value by using the above formula (2) is shown in Table 24.

When the results of comparative example 30 and the results of comparative example 19 are compared, it is understood that: in the case of Escherichia coli in which the function of pyruvate kinase is not impaired, the yield of adipic acid is reduced in the case where the activity of PEP carboxylase is enhanced as compared with the case where the activity is not enhanced, even under anaerobic conditions.

[ Table 24]

Example 19

Preparation of Gene variants of Serratia microorganism having damaged pyruvate kinase Gene and phosphotransferase enzyme Gene

A microorganism variant of serratia having impaired functions of pyruvate kinase and phosphotransferase enzymes was prepared by destroying the gene ptsG encoding the phosphotransferase possessed by the Sg Δ PP strain prepared in example 1.

PCR was carried out using pKD4 as a template and the oligo DNAs of SEQ ID Nos. 244 and 245 as primers to obtain a PCR fragment of 1.6kb in sequence length for disrupting ptsG. After pKD46 was introduced into this strain, a PCR fragment for pstsG disruption was introduced. Colony direct PCR was performed using the obtained kanamycin-resistant strain, and the damage of the target gene and the insertion of the kanamycin-resistant gene were confirmed from the band length. The primers used were the oligo DNAs of SEQ ID Nos. 224 and 247.

Subsequently, pKD46 was released to obtain an ampicillin-sensitive strain. An ampicillin-resistant strain was again obtained by introducing pCP20 into the ampicillin-sensitive strain. The obtained strain was subjected to colony direct PCR, and the shedding of the kanamycin resistance gene was confirmed from the band length. The primers used were oligomeric DNA of SEQ ID Nos. 246 and 247. pCP20 was detached from the kanamycin-sensitive strain. The resulting strain was designated as Sg. DELTA.PPG below.

Example 20

Preparation of Gene variants of Serratia microorganism into which a plasmid was introduced which had a pyruvate kinase gene and a phosphotransferase enzyme gene disrupted and which expressed PEP carboxylase and an enzyme catalyzing reactions A, B, E and F

The plasmid pBBR1MCS-2:, ATCTOR1PCK, prepared in reference example 5 was introduced into the Sg. DELTA.PPG strain prepared in example 19 in the same manner as in example 2, and the resultant microorganism variant of Serratia was Sg. DELTA.PPG/3 HA1 PCK.

Example 21

Production of 3-hydroxyadipic acid and alpha-hydrohexadiene diacid Using a variant of a microorganism of the genus Serratia, which has been introduced with a plasmid that impairs the function of pyruvate kinase and phosphotransferase-like enzymes and that expresses PEP carboxylase and an enzyme that catalyzes reactions A, B, E and F

Production tests of 3-hydroxyadipic acid and α -hydrogenated adipic acid were carried out in the same manner as in example 15 using the serratia microorganism variant prepared in example 20.

Comparative example 31

Production of 3-hydroxyadipic acid and alpha-hydrohexadiene diacid Using a variant of a microorganism of the genus Serratia, which has been introduced with a plasmid that does not impair the function of pyruvate kinase and phosphotransferase-like enzymes and that expresses PEP carboxylase and an enzyme that catalyzes reactions A, B, E and F

Production tests of 3-hydroxyadipic acid and α -hydrohexadiene diacid were carried out in the same manner as in comparative example 13 using the Sg/3HA1PCK strain prepared in reference example 7.

When the results of example 21 were compared with those of example 15, it was found that: the yields of 3-hydroxyadipic acid and alpha-hydrohexadiene diacid are further improved in a microorganism variant of the genus Serratia which has impaired pyruvate kinase and phosphotransferase-like enzymes and which has enhanced PEP carboxylation kinase and enzymatic activity catalyzing the reduction of 3-oxoadipyl-CoA to 3-hydroxyadipyl-CoA. When the results of example 21 and comparative example 31 were compared, it was found that: in variants with impaired genes for pyruvate kinase and phosphotransferase enzymes, the yield of acetate and ethanol produced by conversion of acetyl-CoA is also increased.

[ Table 25]

Example 22

Preparation of Escherichia coli variant having damaged pyruvate kinase Gene and phosphotransferase enzyme Gene

By disrupting the gene ptsG encoding the phosphotransferase of the Ec Δ PP strain produced in example 4, a coliform variant having impaired functions of pyruvate kinase and phosphotransferase enzymes was produced.

PCR was carried out using pKD4 as a template and the oligo DNAs of SEQ ID Nos. 248 and 249 as primers to obtain a PCR fragment of 1.6kb in sequence length for disrupting ptsG. After pKD46 was introduced into this strain, a PCR fragment for pstsG disruption was introduced. Colony direct PCR was performed using the obtained kanamycin-resistant strain, and the damage of the target gene and the insertion of the kanamycin-resistant gene were confirmed from the band length. The primers used were the oligo DNAs of SEQ ID Nos. 224 and 251.

Subsequently, pKD46 was released to obtain an ampicillin-sensitive strain. An ampicillin-resistant strain was again obtained by introducing pCP20 into the ampicillin-sensitive strain. The obtained strain was subjected to colony direct PCR, and the shedding of the kanamycin resistance gene was confirmed from the band length. The primers used were the oligo DNAs of SEQ ID Nos. 250 and 251. pCP20 was detached from the kanamycin-sensitive strain. The resulting strain was hereinafter referred to as Ec Δ PPG.

Example 23

Preparation of Escherichia coli variant having plasmid introduced thereinto which has pyruvate kinase gene and phosphotransferase enzyme gene damaged and which expresses PEP carboxylase and enzyme catalyzing reactions A, B, E and F

The EcΔ PPG strain prepared in example 22 was introduced into pBBR1MCS-2:: ATCTOR1PCK prepared in reference example 5 by the same method as in example 5, and the obtained Escherichia coli variant was EcΔ PPG/3HA1 PCK.

Example 24

Test for production of 3-hydroxyadipic acid and α -hydrohexadiene diacid Using a variant of Escherichia coli into which a plasmid was introduced which impaired the function of pyruvate kinase and phosphotransferase-like enzymes and which expresses PEP carboxylase and an enzyme catalyzing reactions A, B, E and F

Production tests of 3-hydroxyadipic acid and α -hydrohexadiene diacid were carried out in the same manner as in example 16 using the Escherichia coli variants prepared in example 23.

Comparative example 32

Test for production of 3-hydroxyadipic acid and α -hydrohexadiene diacid Using a variant of Escherichia coli into which a plasmid was introduced which did not impair the function of pyruvate kinase and phosphotransferase-like enzymes and which expresses PEP carboxylase and an enzyme catalyzing reactions A, B, E and F

Production tests of 3-hydroxyadipic acid and α -hydrogenated adipic acid were carried out in the same manner as in comparative example 15 using Ec/3HA1 prepared in reference example 9.

When the results of example 24 were compared with those of example 16, it was found that: in a variant of Escherichia coli in which genes for pyruvate kinase and phosphotransferase-like enzymes are impaired and the activity of enzymes catalyzing the reduction of 3-oxoadipyl-CoA to 3-hydroxyadipyl-CoA is enhanced, the yields of 3-hydroxyadipic acid and a-hydrogenated adipic acid are further improved. When the results of example 24 and comparative example 32 are compared, it is understood that: in variants with impaired genes for pyruvate kinase and phosphotransferase enzymes, the yield of acetate and ethanol produced by conversion of acetyl-CoA is also increased.

[ Table 26]

Sequence listing

<110> Dongli corporation

<120> genetically modified microorganism and method for producing 3-hydroxyadipic acid, alpha-hydrogenated adipic acid and/or adipic acid

<130> PF670-PCT

<160> 251

<170> PatentIn version 3.5

<210> 1

<211> 509

<212> PRT

<213> Serratia marcescens ATCC13880

<400> 1

Met Ala Glu Ser Asn Ala Ala Ile Gln Ser Ala Ala Ile Ile Gly Ala

1 5 10 15

Gly Thr Met Gly Arg Gly Ile Ala Tyr Leu Phe Ala Gln Lys Gly Ile

20 25 30

Arg Thr Val Leu Tyr Asn Arg Asn Gly Asn Thr Leu Asn Gln Ala Arg

35 40 45

Glu Tyr Ile Ala Gln Asp Leu Asn Lys Lys Val Glu Gln Gly Lys Ile

50 55 60

Ala Leu Gln Asp Lys Gly Ala Val Leu Ala Asn Leu Met Phe Thr Ser

65 70 75 80

Val Phe Glu Ala Ile Ala Asp Ser Glu Leu Val Ile Glu Thr Ile Ala

85 90 95

Glu Gln Glu Gln Thr Lys Leu Glu Val Leu Ala Ala Ile Ala Ala Val

100 105 110

Val Lys Pro Asp Thr Leu Ile Ala Thr Asn Thr Ser Ser Leu Ser Leu

115 120 125

Asn Lys Leu Ala Thr Ala Val Thr His Ser Glu Arg Phe Ile Gly Leu

130 135 140

His Phe Phe Asn Pro Ala Pro Leu Met Lys Leu Ile Glu Ile Ile Pro

145 150 155 160

Ala Tyr Phe Thr Ala His Ala Thr Thr Glu Arg Cys Arg Gln Leu Val

165 170 175

Ala Ala Leu Gly Lys His Asp Val Val Cys Gln Ala Thr Pro Gly Phe

180 185 190

Ile Val Asn Arg Met Ala Arg Pro Tyr Tyr Leu Glu Gly Phe Arg Leu

195 200 205

Leu Glu Glu His Val Ala Arg Ala Ala Gln Ile Asp Arg Ala Leu Lys

210 215 220

Ala Gly Gly Arg Phe Arg Met Gly Pro Leu Glu Leu Thr Asp Phe Ile

225 230 235 240

Gly Gln Asp Ile Asn Tyr Gln Val Ser Arg Gln Ile Trp Gln Asp Met

245 250 255

Gln Tyr Asp Pro Arg Tyr Thr Pro Gly His Leu Gln Arg Ser Leu Val

260 265 270

Asp Ala Gly Leu Leu Gly Lys Lys Asn Gly Arg Ser Tyr Phe Ala Ala

275 280 285

Glu Glu Thr Ala Pro Pro Val Thr Ala Ala Ser Asn Ala Asp Val Glu

290 295 300

Thr Leu Arg Val Tyr Gly Glu His Pro Phe Phe Thr Leu Leu Gln Gln

305 310 315 320

Arg Ala Ala Leu Gln Trp Pro Gln Leu Arg Val Glu Gln Arg Pro Ala

325 330 335

Leu Pro Gly Leu Gly Ser Ala Val Gln Ile Asn Asp Ala Phe Thr Val

340 345 350

Ser Ile Thr Asp Gly Arg Thr Ala Ser Gln Leu Ala Glu Gln Thr Ala

355 360 365

Ala Asp Ala Phe Val Val Asp Val Ala Leu Asn Tyr Ala Asp Thr Thr

370 375 380

Tyr Leu Ala Ala Ala His Ser Arg His Ala Ser Ala Ala Asn Lys Ala

385 390 395 400

Leu Phe Leu Arg Leu Leu His Thr Ala Ile Pro Gln Val Glu Phe Ile

405 410 415

Lys Asp Ser Pro Ala Leu Ile Val Ala Arg Val Leu Ser Ser Leu Ile

420 425 430

Asn Glu Ser Val Ile Met Val Glu Ser Gly Val Cys Ser Arg Glu Asp

435 440 445

Ile Asp Val Ala Ala Val Ala Gly Val Asn Tyr Ala Gly Gly Ile Phe

450 455 460

Asp Trp Leu Gly Lys Leu Gly Glu Lys Asn Val Arg Thr Thr Leu Ser

465 470 475 480

Asn Leu Ala Gln Leu Leu His Ala Ala Arg Tyr Ala Pro His Tyr Thr

485 490 495

Leu Leu His Ala Ala Gln Pro Ala Leu Thr Thr Thr Pro

500 505

<210> 2

<211> 509

<212> PRT

<213> Serratia nematodiphila DSM21420

<400> 2

Met Ala Glu Ser Asn Ala Ala Ile Gln Ser Ala Ala Ile Ile Gly Ala

1 5 10 15

Gly Thr Met Gly Arg Gly Ile Ala Tyr Leu Phe Ala Gln Lys Gly Ile

20 25 30

Pro Thr Met Leu Tyr Asn Arg Asn Gly Asn Thr Leu Asn Gln Ala Arg

35 40 45

Glu Tyr Ile Ala Gln Asp Leu Asn Lys Lys Val Glu Gln Gly Lys Ile

50 55 60

Ala Leu Arg Asp Lys Asp Ala Val Leu Ala Asn Leu Met Phe Thr Ser

65 70 75 80

Val Phe Glu Ala Ile Ala Asp Ser Glu Leu Val Ile Glu Thr Ile Ala

85 90 95

Glu Gln Glu Gln Thr Lys Leu Glu Val Leu Ala Ala Ile Ala Ala Val

100 105 110

Val Lys Pro Asp Thr Leu Ile Ala Thr Asn Thr Ser Ser Leu Ser Leu

115 120 125

Asn Lys Leu Ala Thr Ala Val Thr His Ser Glu Arg Phe Ile Gly Leu

130 135 140

His Phe Phe Asn Pro Ala Pro Leu Met Lys Leu Ile Glu Ile Ile Pro

145 150 155 160

Ala Tyr Phe Thr Ala His Ala Thr Thr Glu Arg Cys Arg Gln Leu Val

165 170 175

Ala Ala Leu Gly Lys Arg Asp Val Val Cys Gln Ala Thr Pro Gly Phe

180 185 190

Ile Val Asn Arg Met Ala Arg Pro Tyr Tyr Leu Glu Gly Phe Arg Leu

195 200 205

Leu Glu Glu His Val Ala Arg Ala Ala Gln Ile Asp Arg Ala Leu Lys

210 215 220

Ala Gly Gly Arg Phe Arg Met Gly Pro Leu Glu Leu Thr Asp Phe Ile

225 230 235 240

Gly Gln Asp Ile Asn Tyr Gln Val Ser Arg Gln Ile Trp Gln Asp Met

245 250 255

Gln Tyr Asp Pro Arg Tyr Thr Pro Gly His Leu Gln Arg Ser Leu Val

260 265 270

Asp Ala Gly Leu Leu Gly Lys Lys Asn Gly Arg Ser Tyr Phe Ala Ala

275 280 285

Glu Glu Thr Ala Pro Pro Val Thr Ala Ala Asn Asn Ala Asp Val Glu

290 295 300

Thr Leu Arg Val Tyr Gly Glu His Pro Phe Phe Thr Leu Leu Gln Gln

305 310 315 320

Arg Ala Ala Leu Gln Trp Pro Gln Leu Arg Val Glu Gln Arg Pro Ala

325 330 335

Leu Pro Gly Leu Gly Ala Ala Val Gln Ile Asn Asp Ala Phe Thr Val

340 345 350

Ser Ile Thr Asp Gly Arg Thr Ala Ser Gln Leu Ala Glu Gln Thr Ala

355 360 365

Ala Asp Ala Phe Val Val Asp Leu Ala Leu Asn Tyr Ala Asp Thr Thr

370 375 380

Tyr Leu Val Ala Ala His Ser Arg His Ala Ser Ala Ala Asn Lys Ala

385 390 395 400

Leu Phe Leu Arg Leu Leu His Thr Ala Ile Pro Gln Val Glu Phe Ile

405 410 415

Lys Asp Ser Pro Ala Leu Ile Val Ala Arg Val Leu Ser Ser Leu Ile

420 425 430

Asn Glu Ser Val Ile Met Val Glu Ser Gly Val Cys Ser Arg Glu Asp

435 440 445

Ile Asp Val Ala Ala Val Ala Gly Val Asn Tyr Ala Gly Gly Ile Phe

450 455 460

Asp Trp Leu Gly Lys Leu Gly Glu Lys Asn Val Arg Thr Thr Leu Ser

465 470 475 480

Asn Leu Ala Gln Leu Leu His Ala Ala Arg Tyr Ala Pro His Tyr Thr

485 490 495

Leu Leu His Ala Ala Gln Pro Ala Leu Thr Thr Thr Pro

500 505

<210> 3

<211> 509

<212> PRT

<213> Serratia plymuthica NBRC102599

<400> 3

Met Ala Glu Asn Asn Ser Ala Ile Arg Ser Ala Ala Val Ile Gly Ala

1 5 10 15

Gly Thr Met Gly Arg Gly Ile Ala Tyr Leu Leu Ala Leu Asn Gly Ile

20 25 30

Arg Thr Val Leu Tyr Asn Arg Asn Gly Asn Asn Leu Asn Gln Ala Arg

35 40 45

Asp Tyr Ile Val Ser Asp Leu Asp Arg Lys Ile Asp Asn Gly Lys Ile

50 55 60

Thr Leu Gln Lys Lys Gly Gln Ile Leu Ala Asn Ile Ile Phe Ser Asp

65 70 75 80

Val Phe Asp Ala Ile Thr Asp Ser Asp Leu Val Ile Glu Thr Ile Ala

85 90 95

Glu Asp Glu Gln Thr Lys His Glu Ile Leu Ala Ala Ile Ala Ala Thr

100 105 110

Val Lys Pro Glu Ala Ile Ile Ala Thr Asn Thr Ser Ser Leu Ser Leu

115 120 125

Asn Lys Leu Ala Ala Gly Val Glu Asn Asn Pro Arg Phe Ile Gly Leu

130 135 140

His Phe Phe Asn Pro Ala Pro Leu Met Lys Leu Ile Glu Ile Ile Pro

145 150 155 160

Ser Tyr Phe Thr Ser Arg Ala Thr Ser Leu Arg Cys Gln Gln Leu Val

165 170 175

Thr Ala Leu Gly Lys Gln Phe Val Val Cys Lys Ala Thr Pro Gly Phe

180 185 190

Ile Val Asn Arg Met Ala Arg Pro Phe Tyr Leu Glu Gly Phe Arg Leu

195 200 205

Leu Glu Glu Asn Val Ala Leu Ala Pro Gln Ile Asp Arg Ala Leu Lys

210 215 220

Ala Gly Gly His Phe Arg Met Gly Pro Leu Glu Leu Thr Asp Phe Ile

225 230 235 240

Gly Gln Asp Ile Asn Tyr Gln Val Ser Lys Gln Ile Trp Gln Asp Met

245 250 255

Gln Phe Asp Pro Arg Tyr Thr Pro Gly His Leu Gln Arg Ser Leu Val

260 265 270

Asp Ala Gly Leu Leu Gly Arg Lys Asn Gly Arg Ser Phe Phe Ala Ser

275 280 285

Gln Pro Ala Thr Pro Pro Asn Pro Thr Thr Glu Gly Asp Thr Pro Thr

290 295 300

Ser Leu His Phe Tyr Gly Glu His Ala Leu Phe Asp His Leu Gln Gln

305 310 315 320

Arg Ala Leu Ala Thr Trp Pro Ala Leu Arg Val Gln Arg Leu Pro Glu

325 330 335

Arg Pro Glu Leu Gly Arg Phe Ile Leu Val Asn Asn Arg Leu Ala Ile

340 345 350

Lys Ile Thr Asp Gly Arg Thr Ala Asn Leu Leu Ala Gly Leu Thr Ala

355 360 365

Leu Asp Thr Phe Val Ile Asp Ala Ala Leu Asn Tyr Ala Asp Thr Ala

370 375 380

Tyr Leu Val Ala Ala His Asn Gln His Ala Thr Glu Thr Asn Lys Ala

385 390 395 400

Leu Phe Leu Thr Leu Leu Gln Thr Leu Ile Ala Gln Val Glu Phe Ile

405 410 415

Lys Asp Ser Pro Ala Leu Ile Val Ala Arg Val Leu Ser Ser Leu Ile

420 425 430

Asn Glu Ser Val Ile Met Val Glu Ser Gly Val Cys Ser Arg Ala Asp

435 440 445

Ile Asp Ile Ala Ala Val Ala Gly Val Asn Tyr Ala Asp Gly Ile Phe

450 455 460

Ala Trp Leu Ala Gln Leu Gly Gln Lys Asn Val Lys Ser Thr Leu Asp

465 470 475 480

Asn Met Ala Gln Leu Leu His Ser Thr Arg Tyr Tyr Pro His Tyr Ser

485 490 495

Leu Leu Asn Ala Ala Arg Pro Glu Leu Ala Val Ala Pro

500 505

<210> 4

<211> 509

<212> PRT

<213> Serratia proteamaculans 568

<400> 4

Met Ala Glu Asn Asn Ser Ala Ile His Ser Val Ala Val Ile Gly Ala

1 5 10 15

Gly Thr Met Gly Arg Gly Ile Ala Tyr Leu Leu Ala Gln Asn Gly Ile

20 25 30

Arg Thr Leu Leu Tyr Asn Arg Ser Gly Asn Asn Leu Asn Gln Ala Arg

35 40 45

Asp Tyr Ile Ile Arg Asp Leu Asp Lys Lys Ile Asp Gly Gly Lys Ile

50 55 60

Ser Pro Gln Lys Lys Gly Glu Ile Leu Ala Asn Leu Val Phe Ser Pro

65 70 75 80

Ile Phe Glu Ala Ile Ala Asp Ser Asp Leu Val Ile Glu Thr Ile Ala

85 90 95

Glu His Glu Ala Thr Lys His Glu Ile Leu Ala Ala Ile Ala Ala Thr

100 105 110

Val Lys Lys Glu Ala Ile Ile Ala Thr Asn Thr Ser Ser Leu Ser Leu

115 120 125

Asn Lys Leu Ala Ala Gly Val Glu Asn Asn Ala Arg Phe Ile Gly Leu

130 135 140

His Phe Phe Asn Pro Ala Pro Leu Met Lys Leu Ile Glu Ile Ile Pro

145 150 155 160

Ser Tyr Phe Thr Ser Arg Ala Thr Ser Leu Arg Cys Gln Gln Leu Val

165 170 175

Thr Ala Ile Gly Lys Gln Phe Val Val Cys Lys Ala Thr Pro Gly Phe

180 185 190

Ile Val Asn Arg Met Ala Arg Pro Phe Tyr Leu Glu Gly Phe Arg Leu

195 200 205

Leu Glu Glu Asn Val Ala Leu Ala Pro Gln Ile Asp Arg Ala Leu Lys

210 215 220

Ala Gly Gly His Phe Arg Met Gly Pro Leu Glu Leu Thr Asp Phe Ile

225 230 235 240

Gly Gln Asp Ile Asn Tyr Gln Val Ser Ser Gln Ile Trp Gln Asp Met

245 250 255

Gln Tyr Asp Pro Arg Tyr Thr Pro Gly His Leu Gln Arg Ser Leu Val

260 265 270

Asp Ala Gly Leu Leu Gly Lys Lys Asn Gly Arg Ser Phe Phe Thr Pro

275 280 285

Ser Ser Ala Glu Pro Ser Ser Ala Asp Ala Gly Ser Gly Thr Pro Thr

290 295 300

Ser Leu Asn Phe Tyr Gly Glu His Pro Leu Phe Asp Leu Leu Gln Gln

305 310 315 320

Arg Ala Leu Ala Leu Trp Pro Arg Val Gln Ile Asn Arg Gln Ser Glu

325 330 335

Gln Pro Thr Leu Gly Arg Phe Ile Arg Val Asn Asp Ala Met Ala Ile

340 345 350

Lys Ile Thr Asp Gly Arg Thr Ala Asn Leu Leu Ala Glu Leu Thr Glu

355 360 365

Leu Asp Thr Phe Val Ile Asp Ala Ala Leu Asn Tyr Ala Asp Thr Ala

370 375 380

Tyr Leu Ala Ala Ala His Ser Gln Asp Ala Ser Ala Ala Asn Lys Ala

385 390 395 400

Leu Phe Leu Thr Leu Leu Gln Thr Leu Ile Pro Gln Val Glu Phe Ile

405 410 415

Lys Asp Ser Pro Gly Leu Ile Val Ala Arg Val Leu Ser Ser Leu Ile

420 425 430

Asn Glu Ser Val Ile Met Val Glu Ser Gly Val Cys Ser Arg Ala Asp

435 440 445

Ile Asp Ile Ala Ala Val Ala Gly Val Asn Tyr Ala Asp Gly Ile Phe

450 455 460

Ser Trp Leu Ala Gln Leu Gly Gln Lys Asn Val Lys Ser Thr Leu Asp

465 470 475 480

Asn Met Ala Gln Leu Leu His Ser Ala Arg Tyr Tyr Pro His Tyr Ser

485 490 495

Leu Leu Asn Thr Pro Arg Pro Glu Leu Ala Val Ala Pro

500 505

<210> 5

<211> 509

<212> PRT

<213> Serratia ureilytica Lr5/4

<400> 5

Met Ala Glu Ser Asn Ala Ala Ile Gln Ser Ala Ala Ile Ile Gly Ala

1 5 10 15

Gly Thr Met Gly Arg Gly Ile Ala Tyr Leu Leu Ala Gln Lys Ser Ile

20 25 30

Arg Thr Val Leu Tyr Asn Arg Asn Gly Asn Thr Leu Asn Gln Ala Arg

35 40 45

Asp Ala Ile Val Gln Asp Leu Asn Lys Lys Val Glu Gln Gly Lys Leu

50 55 60

Ala Leu Gln Asp Lys Asp Ala Val Leu Ala Asn Leu Thr Phe Ser Ala

65 70 75 80

Glu Phe Gly Ala Ile Ala Asp Ser Asp Leu Val Ile Glu Thr Ile Ala

85 90 95

Glu His Glu Gln Ala Lys Leu Glu Val Leu Ala Ala Ile Ala Ala Thr

100 105 110

Val Lys Pro Asp Thr Leu Ile Ala Thr Asn Thr Ser Ser Leu Ser Leu

115 120 125

Asn Lys Leu Ala Thr Ala Val Thr His Ser Glu Arg Phe Ile Gly Leu

130 135 140

His Phe Phe Asn Pro Ala Pro Leu Met Lys Leu Ile Glu Ile Ile Pro

145 150 155 160

Ala Tyr Phe Thr Ala Gln Val Thr Thr Glu Arg Cys Arg Gln Leu Val

165 170 175

Ala Ala Leu Gly Lys Arg Asp Val Val Cys Gln Ala Thr Pro Gly Phe

180 185 190

Ile Val Asn Arg Met Ala Arg Pro Tyr Tyr Leu Glu Gly Phe Arg Leu

195 200 205

Leu Glu Glu His Val Ala Arg Ala Pro Gln Ile Asp Arg Ala Leu Lys

210 215 220

Ala Gly Gly His Phe Arg Met Gly Pro Leu Glu Leu Thr Asp Phe Ile

225 230 235 240

Gly Gln Asp Ile Asn Tyr Gln Val Ser Arg Gln Ile Trp Gln Asp Met

245 250 255

Gln Tyr Asp Pro Arg Tyr Thr Pro Gly His Leu Gln Arg Ser Leu Val

260 265 270

Asp Ala Gly Leu Leu Gly Lys Lys Asn Gly Arg Ser Tyr Phe Ser Ala

275 280 285

Glu Glu Ser Pro Pro Pro Leu Ala Ala Ala Val Asp Ala Glu Val Glu

290 295 300

Thr Leu Arg Ile Tyr Gly Glu His Pro Leu Phe Thr Leu Leu Gln Gln

305 310 315 320

Arg Ala Thr Leu Gln Trp Pro Arg Leu Arg Val Glu Gln Arg Pro Thr

325 330 335

Leu Pro Gly Leu Gly Ala Ala Ile Gln Val Asn Asp Ala Phe Thr Val

340 345 350

Ser Val Thr Asp Gly Arg Thr Ala Asn Gln Leu Ala Glu Gln Thr Ala

355 360 365

Ala Asp Ala Phe Val Val Asp Val Ala Leu Asn Tyr Gly Asp Thr Ala

370 375 380

Tyr Leu Val Ala Ala His Ser Arg His Ala Ser Ala Ala Asn Lys Ala

385 390 395 400

Leu Phe Leu Arg Leu Leu His Thr Ala Ile Pro Gln Val Glu Phe Ile

405 410 415

Lys Asp Ser Pro Ala Leu Ile Val Ala Arg Val Leu Ser Ser Leu Ile

420 425 430

Asn Glu Ser Val Ile Met Val Glu Ser Gly Val Cys Ser Arg Glu Asp

435 440 445

Ile Asp Val Ala Ala Val Ala Gly Val Asn Tyr Ala Asp Gly Ile Phe

450 455 460

Gly Trp Leu Thr Arg Leu Gly Glu Glu Asn Val Arg Thr Thr Leu Ser

465 470 475 480

Asn Leu Ala Gln Leu Leu His Ala Ala Arg Tyr Ala Pro His Tyr Thr

485 490 495

Leu Leu His Ala Ala Gln Pro Ala Leu Thr Thr Thr Pro

500 505

<210> 6

<211> 509

<212> PRT

<213> Serratia sp. BW106

<400> 6

Met Ala Glu Asn Asn Ser Ala Ile His Ser Val Ala Val Ile Gly Ala

1 5 10 15

Gly Thr Met Gly Arg Gly Ile Ala Tyr Leu Leu Ala Gln Asn Gly Ile

20 25 30

Arg Thr Leu Leu Tyr Asn Arg Ser Gly Asn Asn Leu Asp Gln Ala Arg

35 40 45

Asp Tyr Ile Ile Arg Asp Leu Asp Lys Lys Ile Asp Asn Gly Lys Ile

50 55 60

Ser Gln Gln Lys Lys Gly Glu Val Leu Ala Asn Leu Val Phe Ser Pro

65 70 75 80

Ile Phe Asp Ala Ile Ala Asp Ser Asp Leu Val Ile Glu Thr Ile Ala

85 90 95

Glu His Glu Thr Thr Lys His Glu Ile Leu Ala Ala Ile Ala Ala Thr

100 105 110

Val Lys Gln Glu Ala Ile Ile Ala Thr Asn Thr Ser Ser Leu Ser Leu

115 120 125

Asn Lys Leu Ala Ala Gly Val Glu Asn Asn Ala Arg Phe Ile Gly Leu

130 135 140

His Phe Phe Asn Pro Ala Pro Leu Met Lys Leu Ile Glu Ile Ile Pro

145 150 155 160

Ser Tyr Phe Thr Ser Arg Ala Thr Ser Leu Arg Cys Gln Gln Leu Val

165 170 175

Thr Ala Leu Gly Lys Gln Phe Val Val Cys Lys Ala Thr Pro Gly Phe

180 185 190

Ile Val Asn Arg Met Ala Arg Pro Phe Tyr Leu Glu Gly Phe Arg Leu

195 200 205

Leu Glu Glu Asn Val Ala Leu Ala Pro Gln Ile Asp Arg Ala Leu Lys

210 215 220

Ala Gly Gly His Phe Arg Met Gly Pro Leu Glu Leu Thr Asp Phe Ile

225 230 235 240

Gly Gln Asp Ile Asn Tyr Gln Val Ser Ser Gln Ile Trp Gln Asp Met

245 250 255

Gln Tyr Asp Pro Arg Tyr Thr Pro Gly His Leu Gln Arg Ser Leu Val

260 265 270

Asp Ala Gly Leu Leu Gly Lys Lys Asn Gly Arg Ser Phe Phe Ala Ala

275 280 285

Pro Ser Ser Glu Ser Asn Pro Leu Asp Ala Gly Asn Gly Thr Leu Thr

290 295 300

Ser Leu His Phe Tyr Gly Glu His Thr Leu Phe Asp Leu Leu Gln Gln

305 310 315 320

Arg Ala Leu Ala Ile Trp Pro Thr Leu Gln Ile Ile His Gln Pro Glu

325 330 335

Arg Pro Thr Leu Gly Arg Phe Ile Arg Val Asn Asp Ala Leu Ala Val

340 345 350

Lys Ile Thr Asp Gly Arg Thr Ala Asn Leu Leu Ala Glu Leu Thr Asp

355 360 365

Leu Asp Thr Phe Val Ile Asp Ala Ala Leu Asn Tyr Ser Asp Thr Ala

370 375 380

Tyr Leu Val Ala Ala His Asn Gln Asp Ala Ala Glu Ala Asn Lys Ala

385 390 395 400

Leu Phe Leu Ser Leu Leu Gln Thr Leu Ile Pro Gln Val Glu Phe Ile

405 410 415

Lys Asp Ser Pro Gly Leu Ile Val Ala Arg Val Leu Ser Ser Leu Ile

420 425 430

Asn Glu Ser Val Ile Met Val Glu Ser Gly Val Cys Ser Arg Ala Asp

435 440 445

Ile Asp Ile Ala Ala Val Ala Gly Val Asn Tyr Ala Asp Gly Ile Phe

450 455 460

Ala Trp Leu Thr Gln Leu Gly Gln Lys Asn Val Lys Ser Thr Leu Asp

465 470 475 480

Asn Met Ala Gln Leu Leu His Ser Ala Arg Tyr Tyr Pro His Tyr Ser

485 490 495

Leu Leu Asn Ala Pro Arg Pro Glu Leu Ala Val Ala Pro

500 505

<210> 7

<211> 509

<212> PRT

<213> Serratia liquefaciens FK01

<400> 7

Met Ala Glu Asn Asn Thr Ala Ile Asp Ser Val Ala Val Ile Gly Ala

1 5 10 15

Gly Thr Met Gly Arg Gly Ile Ala Tyr Leu Leu Ala Leu Asn Gly Ile

20 25 30

Arg Thr Leu Leu Tyr Asn Arg Asn Gly Asn His Leu Asn Gln Ala Arg

35 40 45

Asp Tyr Ile Val Gly Asp Leu Asp Lys Lys Ile Asp Asn Gly Lys Ile

50 55 60

Ser Gln Gln Lys Lys Gly Glu Val Leu Ala Asn Leu Val Phe Ser Pro

65 70 75 80

Val Phe Asp Ala Ile Ala Asp Ser Asp Leu Val Ile Glu Thr Ile Ala

85 90 95

Glu His Glu Pro Thr Lys His Glu Ile Leu Ala Ala Ile Ala Ala Thr

100 105 110

Val Lys Lys Glu Ala Ile Ile Ala Thr Asn Thr Ser Ser Leu Ser Leu

115 120 125

Asn Lys Leu Ala Ala Gly Ile Glu Asn Asn Ser Arg Phe Ile Gly Leu

130 135 140

His Phe Phe Asn Pro Ala Pro Leu Met Lys Leu Ile Glu Ile Ile Pro

145 150 155 160

Ser Tyr Phe Thr Ala Arg Ala Thr Thr Leu Arg Cys Gln Gln Leu Val

165 170 175

Thr Ala Ile Gly Lys Lys Phe Val Val Cys Lys Ala Thr Pro Gly Phe

180 185 190

Ile Val Asn Arg Met Ala Arg Pro Phe Tyr Leu Glu Gly Phe Arg Leu

195 200 205

Leu Glu Glu Asn Val Ala Leu Ala Pro Gln Ile Asp Arg Ala Leu Lys

210 215 220

Ala Gly Gly His Phe Arg Met Gly Pro Leu Glu Leu Thr Asp Phe Ile

225 230 235 240

Gly Gln Asp Ile Asn Tyr Gln Val Ser Ser Gln Ile Trp Gln Asp Met

245 250 255

Gln Tyr Asp Pro Arg Tyr Thr Pro Gly His Leu Gln Arg Ser Leu Val

260 265 270

Asp Ala Gly Leu Leu Gly Lys Lys Asn Gly Arg Ser Phe Phe Ser Pro

275 280 285

Ser Ala Asp Ala Ala Asn Pro Pro Ala Thr Gly Gly Gly Thr Leu Ser

290 295 300

Ser Leu His Phe Phe Gly Glu His Pro Leu Phe Asp Leu Leu Gln Gln

305 310 315 320

His Ala Phe Ala Thr Trp Ala Pro Leu Ala Ile Thr Arg Gln Pro Glu

325 330 335

His Pro Val Leu Gly Arg Phe Ile Gln Val Asn Asp Ser Leu Ala Val

340 345 350

Lys Ile Thr Asp Gly Arg Thr Ala Asn Gln Leu Ala Glu Leu Ala Gly

355 360 365

Leu Asp Thr Phe Val Val Asp Val Ala Leu Asn Tyr Ala Asn Thr Ala

370 375 380

Phe Leu Val Ala Ala His Ser Gln Gln Ala Thr Glu Ala Asn Lys Glu

385 390 395 400

Leu Phe Leu Thr Leu Leu Gln Thr Val Ile Pro Gln Val Glu Phe Val

405 410 415

Lys Asp Ser Pro Gly Leu Ile Val Ala Arg Val Leu Ser Ser Leu Ile

420 425 430

Asn Glu Ser Val Ile Met Val Glu Ser Gly Val Cys Ser Arg Glu Asp

435 440 445

Ile Asp Ile Ala Ala Val Ala Gly Val Asn Tyr Ala Asp Gly Ile Phe

450 455 460

Ala Trp Leu Ala Gln Leu Gly Gln Lys Asn Val Lys Ser Thr Leu Asp

465 470 475 480

Asn Met Ala Gln Leu Leu His Ser Ala Arg Tyr Tyr Pro His Tyr Ser

485 490 495

Leu Leu His Ala Thr Arg Pro Glu Leu Ala Val Ala Pro

500 505

<210> 8

<211> 509

<212> PRT

<213> Serratia sp. S119

<400> 8

Met Ala Glu Ser Asn Ala Ala Ile Gln Ser Ala Ala Ile Ile Gly Ala

1 5 10 15

Gly Thr Met Gly Arg Gly Ile Ala Tyr Leu Phe Ala Gln Lys Gly Ile

20 25 30

Arg Thr Val Leu Tyr Asn Arg Asn Gly Asn Thr Leu Asn Gln Ala Arg

35 40 45

Glu Tyr Ile Ala Gln Asp Leu Asn Lys Lys Val Glu Gln Gly Lys Ile

50 55 60

Ala Leu Gln Asp Lys Gly Ala Val Leu Ala Asn Leu Val Phe Thr Ser

65 70 75 80

Val Phe Glu Thr Ile Ala Asp Ser Asp Leu Val Ile Glu Thr Ile Ala

85 90 95

Glu Gln Glu Gln Thr Lys Leu Glu Val Leu Ala Ala Ile Ala Ala Ala

100 105 110

Val Lys Pro Asp Thr Leu Ile Ala Thr Asn Thr Ser Ser Leu Ser Leu

115 120 125

Asn Lys Leu Ala Thr Ala Val Thr His Ser Glu Arg Phe Ile Gly Leu

130 135 140

His Phe Phe Asn Pro Ala Pro Leu Met Lys Leu Ile Glu Ile Ile Pro

145 150 155 160

Ala Tyr Phe Thr Ala Arg Ala Thr Thr Glu Arg Cys Arg Gln Leu Val

165 170 175

Ala Ala Leu Gly Lys Arg Asp Val Val Cys Gln Ala Thr Pro Gly Phe

180 185 190

Ile Val Asn Arg Met Ala Arg Pro Tyr Tyr Leu Glu Gly Phe Arg Leu

195 200 205

Leu Glu Glu His Val Ala Arg Ala Pro Gln Ile Asp Arg Ala Leu Lys

210 215 220

Ala Gly Gly His Phe Arg Met Gly Pro Leu Glu Leu Thr Asp Phe Ile

225 230 235 240

Gly Gln Asp Ile Asn Tyr Gln Val Ser Arg Gln Ile Trp Gln Asp Met

245 250 255

Gln Tyr Asp Pro Arg Tyr Thr Pro Gly His Leu Gln Arg Ser Leu Val

260 265 270

Asp Ala Gly Leu Leu Gly Lys Lys Asn Gly Arg Ser Tyr Phe Ala Ala

275 280 285

Glu Glu Asn Ala Leu Pro Val Thr Ala Ala Thr Asp Ala Asp Val Glu

290 295 300

Thr Leu Arg Val Tyr Gly Glu His Pro Phe Phe Thr Leu Leu Gln Gln

305 310 315 320

Arg Ala Ala Leu Gln Trp Pro Arg Leu Arg Val Glu Gln Arg Pro Ala

325 330 335

Leu Pro Gly Leu Gly Pro Ala Val Gln Ile Asn Glu Ala Phe Thr Val

340 345 350

Ser Val Thr Asp Gly Arg Thr Ala Ser Gln Leu Ala Glu Leu Thr Ala

355 360 365

Ala Asp Ala Phe Val Val Asp Val Ala Leu Asn Tyr Ala Asp Thr Ala

370 375 380

Tyr Leu Val Ala Ala His Asn Arg His Ala Ser Ala Ala Asn Lys Ala

385 390 395 400

Leu Phe Leu Arg Leu Leu His Thr Ala Ile Pro Gln Val Glu Phe Ile

405 410 415

Lys Asp Ser Pro Ala Leu Ile Val Ala Arg Val Leu Ser Ser Leu Ile

420 425 430

Asn Glu Ser Val Ile Met Val Glu Ser Gly Val Cys Ser Arg Glu Asp

435 440 445

Ile Asn Val Ala Ala Val Ala Gly Val Asn Tyr Ala Asp Gly Ile Phe

450 455 460

Gly Leu Leu Asp Ser Leu Gly Glu Lys Asn Val Arg Thr Thr Leu Ser

465 470 475 480

Asn Leu Ala Gln Leu Leu His Ala Ala Arg Tyr Ala Pro His Tyr Thr

485 490 495

Leu Leu His Ala Ala Gln Pro Ala Leu Thr Thr Thr Pro

500 505

<210> 9

<211> 509

<212> PRT

<213> Serratia sp. YD25

<400> 9

Met Ala Glu Arg Asn Ala Ala Ile Gln Ser Ala Ala Ile Ile Gly Ala

1 5 10 15

Gly Thr Met Gly Arg Gly Ile Ala Tyr Leu Phe Ala Gln Lys Gly Ile

20 25 30

Arg Thr Val Leu Tyr Asn Arg Asn Gly Asn Thr Leu Ile Gln Ala Arg

35 40 45

Glu Tyr Ile Val Gln Asp Leu Asp Lys Lys Val Glu Gln Gly Arg Leu

50 55 60

Ala Pro Gln Asp Lys Asp Ala Val Leu Ala Asn Leu Gln Phe Ser Ser

65 70 75 80

Val Phe Glu Ala Ile Val Asp Ser Asp Leu Val Leu Glu Thr Ile Ala

85 90 95

Glu Gln Glu Gln Ala Lys Leu Glu Val Leu Ala Ala Ile Ala Ala Ala

100 105 110

Val Lys Pro Asp Thr Leu Ile Ala Thr Asn Thr Ser Ser Leu Ser Leu

115 120 125

Asn Lys Leu Ala Thr Ala Val Thr His Ser Glu Arg Phe Ile Gly Leu

130 135 140

His Phe Phe Asn Pro Ala Pro Leu Met Lys Leu Ile Glu Ile Ile Pro

145 150 155 160

Ala Tyr Phe Thr Thr Gln Ala Thr Thr Glu Arg Cys Arg Gln Leu Val

165 170 175

Ala Ala Leu Gly Lys Arg Asp Val Val Cys Gln Ala Thr Pro Gly Phe

180 185 190

Ile Val Asn Arg Met Ala Arg Pro Tyr Tyr Leu Glu Gly Phe Arg Leu

195 200 205

Leu Glu Glu His Val Ala Arg Ala Pro Gln Ile Asp Arg Ala Leu Lys

210 215 220

Ala Gly Gly His Phe Arg Met Gly Pro Leu Glu Leu Thr Asp Phe Ile

225 230 235 240

Gly Gln Asp Ile Asn Tyr Gln Val Ser Arg Gln Ile Trp Gln Asp Met

245 250 255

Gln Tyr Asp Pro Arg Tyr Thr Pro Gly His Leu Gln Arg Ser Leu Val

260 265 270

Asp Ala Gly Leu Leu Gly Lys Lys Asn Gly Arg Ser Tyr Phe Ser Ala

275 280 285

Glu Glu Thr Ala Pro Pro Val Glu Ala Ala Ala Glu Ala Asp Val Glu

290 295 300

Thr Leu Arg Ile Tyr Gly Glu His Pro Leu Phe Thr Leu Leu Gln Gln

305 310 315 320

Arg Ala Ala Leu Gln Trp Pro Gln Leu Arg Val Glu Gln Arg Pro Ala

325 330 335

Leu Ser Gly Leu Gly Ala Ala Ile Gln Val Asn Asp Ala Phe Thr Val

340 345 350

Ser Val Thr Asp Gly Arg Thr Ala Asn Gln Leu Ala Glu Gln Thr Ala

355 360 365

Ala Asp Ala Phe Val Val Asp Val Ala Leu Asn Tyr Ala Asp Thr Ala

370 375 380

Tyr Leu Val Ala Ala His Ser Arg His Ala Ser Ala Ala Asn Lys Ala

385 390 395 400

Leu Phe Leu Arg Leu Leu His Thr Ala Leu Pro Gln Val Glu Phe Ile

405 410 415

Lys Asp Ser Pro Ala Leu Ile Val Ala Arg Val Leu Ser Ser Leu Ile

420 425 430

Asn Glu Ser Val Ile Met Val Glu Ser Gly Val Cys Ser Arg Glu Asp

435 440 445

Ile Asp Val Ala Ala Val Ala Gly Val Asn Tyr Ala Asp Gly Ile Phe

450 455 460

Gly Trp Leu Thr Arg Leu Gly Glu Lys Asn Val Arg Thr Thr Leu Ser

465 470 475 480

Asn Leu Ala Gln Leu Leu His Ala Ala Arg Tyr Ala Pro His Tyr Thr

485 490 495

Leu Leu His Ala Ala Gln Pro Ala Leu Thr Thr Thr Pro

500 505

<210> 10

<211> 509

<212> PRT

<213> Serratia sp. FS14

<400> 10

Met Ala Glu Ser Asn Ala Ala Ile Gln Ser Ala Ala Ile Ile Gly Ala

1 5 10 15

Gly Thr Met Gly Arg Gly Ile Ala Tyr Leu Phe Ala Gln Lys Gly Ile

20 25 30

Pro Thr Met Leu Tyr Asn Arg Asn Gly Asn Thr Leu Asn Gln Ala Arg

35 40 45

Glu Tyr Ile Ala Gln Asp Leu Asn Lys Lys Val Glu Gln Gly Lys Ile

50 55 60

Ala Leu Gln Asp Lys Asp Ala Val Leu Ala Asn Leu Met Phe Thr Ser

65 70 75 80

Val Phe Glu Ala Ile Ala Asp Ser Glu Leu Val Ile Glu Thr Ile Ala

85 90 95

Glu Gln Glu Gln Thr Lys Leu Glu Val Leu Ala Ala Ile Ala Ala Val

100 105 110

Val Lys Pro Asp Thr Leu Ile Ala Thr Asn Thr Ser Ser Leu Ser Leu

115 120 125

Asn Lys Leu Ala Thr Ala Val Thr His Ser Glu Arg Phe Ile Gly Leu

130 135 140

His Phe Phe Asn Pro Ala Pro Leu Met Lys Leu Ile Glu Ile Ile Pro

145 150 155 160

Ala Tyr Phe Thr Ala His Ala Thr Thr Glu Arg Cys Arg Gln Leu Val

165 170 175

Ala Ala Leu Gly Lys Arg Asp Val Val Cys Gln Ala Thr Pro Gly Phe

180 185 190

Ile Val Asn Arg Met Ala Arg Pro Tyr Tyr Leu Glu Gly Phe Arg Leu

195 200 205

Leu Glu Glu His Val Ala Arg Ala Ala Gln Ile Asp Arg Ala Leu Lys

210 215 220

Ala Gly Gly Arg Phe Arg Met Gly Pro Leu Glu Leu Thr Asp Phe Ile

225 230 235 240

Gly Gln Asp Ile Asn Tyr Gln Val Ser Arg Gln Ile Trp Gln Asp Met

245 250 255

Gln Tyr Asp Pro Arg Tyr Thr Pro Gly His Leu Gln Arg Ser Leu Val

260 265 270

Asp Ala Gly Leu Leu Gly Lys Lys Asn Gly Arg Ser Tyr Phe Ala Ala

275 280 285

Glu Glu Thr Ala Pro Pro Val Thr Ala Ala Asn Asn Ala Asp Val Glu

290 295 300

Thr Leu Arg Val Tyr Gly Glu His Pro Phe Phe Thr Leu Leu Gln Gln

305 310 315 320

Arg Ala Ala Leu Gln Trp Pro Gln Leu Arg Val Glu Gln Arg Pro Ala

325 330 335

Leu Pro Gly Leu Gly Ala Ala Val Gln Ile Asn Asp Ala Phe Thr Val

340 345 350

Ser Ile Thr Asp Gly Arg Thr Ala Ser Gln Leu Ala Glu Gln Thr Ala

355 360 365

Ala Asp Ala Phe Val Val Asp Val Ala Leu Asn Tyr Ala Asp Thr Thr

370 375 380

Tyr Leu Val Ala Ala His Ser Arg His Ala Ser Ala Ala Asn Lys Ala

385 390 395 400

Leu Phe Leu Arg Leu Leu His Thr Ala Ile Pro Gln Val Glu Phe Ile

405 410 415

Lys Asp Ser Pro Ala Leu Ile Val Ala Arg Val Leu Ser Ser Leu Ile

420 425 430

Asn Glu Ser Val Ile Met Val Glu Ser Gly Val Cys Ser Arg Glu Asp

435 440 445

Ile Asp Val Ala Ala Val Ala Gly Val Asn Tyr Ala Gly Gly Ile Phe

450 455 460

Asp Trp Leu Gly Lys Leu Gly Glu Lys Asn Val Arg Thr Thr Leu Ser

465 470 475 480

Asn Leu Ala Gln Leu Leu His Ala Ala Arg Tyr Ala Pro His Tyr Thr

485 490 495

Leu Leu His Ala Ala Gln Pro Ala Leu Thr Thr Thr Pro

500 505

<210> 11

<211> 509

<212> PRT

<213> Serratia sp. HMSC15F11

<400> 11

Met Ala Glu Ser Asn Ala Ala Ile Gln Ser Ala Ala Ile Ile Gly Ala

1 5 10 15

Gly Thr Met Gly Arg Gly Ile Ala Tyr Leu Phe Ala Gln Lys Gly Ile

20 25 30

Arg Thr Val Leu Tyr Asn Arg Asn Gly Asn Thr Leu Asn Gln Ala Arg

35 40 45

Asp Tyr Ile Glu Leu Asp Leu Asn Lys Lys Val Glu Gln Gly Lys Ile

50 55 60

Ala Leu Gln Asp Lys Gly Ala Val Leu Ala Asn Leu Val Phe Thr Ser

65 70 75 80

Val Phe Glu Thr Ile Ala Asp Ser Glu Leu Val Ile Glu Thr Ile Ala

85 90 95

Glu Gln Glu Gln Thr Lys Leu Glu Val Leu Ala Ala Ile Ala Ala Ala

100 105 110

Val Lys Pro Asp Thr Leu Ile Ala Thr Asn Thr Ser Ser Leu Ser Leu

115 120 125

Asn Lys Leu Ala Thr Ala Val Thr His Ser Glu Arg Phe Ile Gly Leu

130 135 140

His Phe Phe Asn Pro Ala Pro Leu Met Lys Leu Ile Glu Ile Ile Pro

145 150 155 160

Ala Tyr Phe Thr Ala His Ala Thr Thr Glu Arg Cys Arg Gln Leu Val

165 170 175

Ala Ala Leu Gly Lys Arg Asp Val Val Cys Gln Ala Thr Pro Gly Phe

180 185 190

Ile Val Asn Arg Met Ala Arg Pro Tyr Tyr Leu Glu Gly Phe Arg Leu

195 200 205

Leu Glu Glu His Val Ala Arg Ala Pro Gln Ile Asp Arg Ala Leu Lys

210 215 220

Ala Gly Gly His Phe Arg Met Gly Pro Leu Glu Leu Thr Asp Phe Ile

225 230 235 240

Gly Gln Asp Ile Asn Tyr Gln Val Ser Arg Gln Ile Trp Gln Asp Met

245 250 255

Gln Tyr Asp Ala Arg Tyr Thr Pro Gly His Leu Gln Arg Ser Leu Val

260 265 270

Asp Ala Gly Leu Leu Gly Lys Lys Asn Gly Arg Ser Tyr Phe Ala Val

275 280 285

Glu Glu Asn Ala Pro Pro Val Met Ala Ala Thr Asp Ala Asp Ile Glu

290 295 300

Thr Leu His Val Tyr Gly Glu His Pro Phe Phe Thr Leu Leu Gln Gln

305 310 315 320

Arg Ala Ala Leu Gln Trp Pro Gln Leu Arg Val Glu Gln Arg Pro Ala

325 330 335

Leu Pro Gly Leu Gly Pro Ala Val Arg Ile Asn Asp Ala Phe Thr Val

340 345 350

Ser Ile Thr Asp Gly Arg Thr Ala Asn Gln Leu Ala Glu Gln Thr Ala

355 360 365

Ala Asp Ala Phe Val Val Asp Val Ala Leu Asn Tyr Ala Asp Thr Ala

370 375 380

Tyr Leu Val Ala Ala His Ser Arg His Ala Ser Ala Ala Asn Lys Ala

385 390 395 400

Leu Phe Leu Arg Leu Leu His Thr Ala Ile Pro Gln Val Glu Phe Ile

405 410 415

Lys Asp Ser Pro Ala Leu Ile Val Ala Arg Val Leu Ser Ser Leu Ile

420 425 430

Asn Glu Ser Val Ile Met Val Glu Ser Gly Val Cys Ser Arg Glu Asp

435 440 445

Ile Asp Val Ala Ala Val Ala Gly Val Asn Tyr Ala Asp Gly Ile Phe

450 455 460

Gly Trp Leu Asp Ser Leu Gly Glu Lys Asn Val Arg Thr Thr Leu Ser

465 470 475 480

Asn Leu Ala Gln Leu Leu His Ala Ala Arg Tyr Ala Pro His Tyr Thr

485 490 495

Leu Leu His Ala Ala Gln Pro Ala Leu Thr Thr Thr Pro

500 505

<210> 12

<211> 509

<212> PRT

<213> Serratia sp. JKS000199

<400> 12

Met Ala Glu Ser Asn Ala Ala Ile Gln Ser Ala Ala Ile Ile Gly Ala

1 5 10 15

Gly Thr Met Gly Arg Gly Ile Ala Tyr Leu Leu Ala Gln Lys Ser Ile

20 25 30

Arg Thr Val Leu Tyr Asn Arg Asn Gly Asn Thr Leu Asn Gln Ala Arg

35 40 45

Asp Ala Ile Val Gln Asp Leu Asn Lys Lys Val Glu Gln Gly Lys Leu

50 55 60

Ala Leu Gln Asp Lys Asp Ala Val Leu Ala Asn Leu Thr Phe Ser Thr

65 70 75 80

Glu Phe Gly Ala Ile Ala Asp Ser Asp Leu Val Ile Glu Thr Ile Ala

85 90 95

Glu His Glu Gln Ala Lys Leu Glu Val Leu Ala Ala Ile Ala Ala Thr

100 105 110

Val Lys Pro Asp Thr Leu Ile Ala Thr Asn Thr Ser Ser Leu Ser Leu

115 120 125

Asn Lys Leu Ala Thr Ala Val Thr His Ser Glu Arg Phe Ile Gly Leu

130 135 140

His Phe Phe Asn Pro Ala Pro Leu Met Lys Leu Ile Glu Ile Ile Pro

145 150 155 160

Ala Tyr Phe Thr Ala Gln Val Thr Thr Glu Arg Cys Arg Gln Leu Val

165 170 175

Ala Ala Leu Gly Lys Arg Asp Val Val Cys Gln Ala Thr Pro Gly Phe

180 185 190

Ile Val Asn Arg Met Ala Arg Pro Tyr Tyr Leu Glu Gly Phe Arg Leu

195 200 205

Leu Glu Glu His Val Ala Arg Ala Pro Gln Ile Asp Arg Ala Leu Lys

210 215 220

Ala Gly Gly His Phe Arg Met Gly Pro Leu Glu Leu Thr Asp Phe Ile

225 230 235 240

Gly Gln Asp Ile Asn Tyr Gln Val Ser Arg Gln Ile Trp Gln Asp Met

245 250 255

Gln Tyr Asp Pro Arg Tyr Thr Pro Gly His Leu Gln Arg Ser Leu Val

260 265 270

Asp Ala Gly Leu Leu Gly Lys Lys Asn Gly Arg Ser Tyr Phe Ser Ala

275 280 285

Glu Glu Ser Pro Pro Pro Leu Ala Ala Ala Val Asp Ala Glu Val Glu

290 295 300

Thr Leu Arg Ile Tyr Gly Glu His Pro Leu Phe Thr Leu Leu Gln Gln

305 310 315 320

Arg Ala Ala Leu Gln Trp Pro Arg Leu Arg Val Glu Gln Arg Pro Thr

325 330 335

Leu Pro Gly Leu Gly Ala Ala Ile Gln Val Asn Asp Ala Phe Thr Val

340 345 350

Ser Val Thr Asp Gly Arg Thr Ala Asn Gln Leu Ala Glu Gln Thr Ala

355 360 365

Ala Asp Ala Phe Val Val Asp Val Ala Leu Asn Tyr Gly Asp Thr Ala

370 375 380

Tyr Leu Val Ala Ala His Ser Arg His Ala Ser Ala Ala Asn Lys Ala

385 390 395 400

Leu Phe Leu Arg Leu Leu His Thr Ala Ile Pro Leu Val Glu Phe Ile

405 410 415

Lys Asp Ser Pro Ala Leu Ile Val Ala Arg Val Leu Ser Ser Leu Ile

420 425 430

Asn Glu Ser Val Ile Met Val Glu Ser Gly Val Cys Ser Arg Glu Asp

435 440 445

Ile Asp Val Ala Ala Val Ala Gly Val Asn Tyr Ala Asp Gly Ile Phe

450 455 460

Gly Trp Leu Thr Arg Leu Gly Glu Glu Asn Val Arg Thr Thr Leu Ser

465 470 475 480

Asn Leu Ala Gln Leu Leu His Ala Ala Arg Tyr Ala Pro His Tyr Thr

485 490 495

Leu Leu His Ala Ala Gln Pro Ala Leu Thr Thr Thr Pro

500 505

<210> 13

<211> 509

<212> PRT

<213> Serratia sp. TEL

<400> 13

Met Ala Glu Ser Asn Ala Ala Ile Gln Ser Ala Ala Ile Ile Gly Ala

1 5 10 15

Gly Thr Met Gly Arg Gly Ile Ala Tyr Leu Leu Ala Gln Lys Ser Ile

20 25 30

Arg Thr Val Leu Tyr Asn Arg Asn Gly Asn Thr Leu Asn Gln Ala Arg

35 40 45

Asp Ala Ile Val Gln Asp Leu Asn Lys Lys Val Glu Gln Gly Lys Leu

50 55 60

Ala Leu Gln Asp Lys Asp Ala Val Leu Ala Asn Leu Thr Phe Ser Ala

65 70 75 80

Glu Phe Gly Ala Ile Ala Asp Ser Asp Leu Val Ile Glu Thr Ile Ala

85 90 95

Glu His Glu Gln Ala Lys Leu Glu Val Leu Ala Ala Ile Ala Ala Thr

100 105 110

Val Lys Pro Asp Thr Leu Ile Ala Thr Asn Thr Ser Ser Leu Ser Leu

115 120 125

Asn Lys Leu Ala Thr Ala Val Thr His Ser Glu Arg Phe Ile Gly Leu

130 135 140

His Phe Phe Asn Pro Ala Pro Leu Met Lys Leu Ile Glu Ile Ile Pro

145 150 155 160

Ala Tyr Phe Thr Ala Gln Val Thr Thr Glu Arg Cys Arg Gln Leu Val

165 170 175

Ala Ala Leu Gly Lys Arg Asp Val Val Cys Gln Ala Thr Pro Gly Phe

180 185 190

Ile Val Asn Arg Met Ala Arg Pro Tyr Tyr Leu Glu Gly Phe Arg Leu

195 200 205

Leu Glu Glu His Val Ala Arg Ala Pro Gln Ile Asp Arg Ala Leu Lys

210 215 220

Ala Gly Gly His Phe Arg Met Gly Pro Leu Glu Leu Thr Asp Phe Ile

225 230 235 240

Gly Gln Asp Ile Asn Tyr Gln Val Ser Arg Gln Ile Trp Gln Asp Met

245 250 255

Gln Tyr Asp Pro Arg Tyr Thr Pro Gly His Leu Gln Arg Ser Leu Val

260 265 270

Asp Ala Gly Leu Leu Gly Lys Lys Asn Gly Arg Ser Tyr Phe Ser Ala

275 280 285

Glu Glu Ser Pro Pro Pro Leu Ala Ala Ala Val Asp Ala Glu Val Glu

290 295 300

Thr Leu Arg Ile Tyr Gly Glu His Pro Leu Phe Thr Leu Leu Gln Gln

305 310 315 320

Arg Ala Thr Leu Gln Trp Pro Arg Leu Arg Val Glu Gln Arg Pro Thr

325 330 335

Leu Pro Gly Leu Gly Ala Ala Ile Gln Val Asn Asp Ala Phe Thr Val

340 345 350

Ser Val Thr Asp Gly Arg Thr Ala Asn Gln Leu Ala Glu Gln Thr Ala

355 360 365

Ala Asp Ala Phe Val Val Asp Val Ala Leu Asn Tyr Gly Asp Thr Ala

370 375 380

Tyr Leu Val Ala Ala His Ser Arg His Ala Ser Ala Ala Asn Lys Ala

385 390 395 400

Leu Phe Leu Arg Leu Leu His Thr Ala Ile Pro Gln Val Glu Phe Ile

405 410 415

Lys Asp Ser Pro Ala Leu Ile Val Ala Arg Val Leu Ser Ser Leu Ile

420 425 430

Asn Glu Ser Val Ile Met Val Glu Ser Gly Val Cys Ser Arg Glu Asp

435 440 445

Ile Asp Val Ala Ala Val Ala Gly Val Asn Tyr Ala Asp Gly Ile Phe

450 455 460

Gly Trp Leu Thr Arg Leu Gly Glu Glu Asn Val Arg Ala Thr Leu Ser

465 470 475 480

Asn Leu Ala Gln Leu Leu His Ala Ala Arg Tyr Ala Pro His Tyr Thr

485 490 495

Leu Leu His Ala Ala Gln Pro Ala Leu Thr Thr Thr Pro

500 505

<210> 14

<211> 509

<212> PRT

<213> Serratia sp. ISTD04

<400> 14

Met Ala Glu Ser Asn Ala Ala Ile Gln Ser Ala Ala Ile Ile Gly Ala

1 5 10 15

Gly Thr Met Gly Arg Gly Ile Ala Tyr Leu Leu Ala Gln Lys Ser Ile

20 25 30

Arg Thr Val Leu Tyr Asn Arg Asn Gly Asn Thr Leu Asn Gln Ala Arg

35 40 45

Asp Ala Ile Val Gln Asp Leu Asn Lys Lys Val Glu Gln Gly Lys Leu

50 55 60

Ala Leu Gln Asp Lys Asp Ala Val Leu Ala Asn Leu Thr Phe Ser Ala

65 70 75 80

Glu Phe Gly Val Ile Ala Asp Ser Asp Leu Val Ile Glu Thr Ile Ala

85 90 95

Glu His Glu Gln Ala Lys Leu Glu Val Leu Ala Ala Ile Ala Ala Thr

100 105 110

Val Lys Ser Asp Thr Leu Ile Ala Thr Asn Thr Ser Ser Leu Ser Leu

115 120 125

Asn Lys Leu Ala Thr Ala Val Thr His Ser Glu Arg Phe Ile Gly Leu

130 135 140

His Phe Phe Asn Pro Ala Pro Leu Met Lys Leu Ile Glu Ile Ile Pro

145 150 155 160

Ala Tyr Phe Thr Ala Gln Val Thr Thr Glu Arg Cys Arg Gln Leu Val

165 170 175

Ala Ala Leu Gly Lys Arg Asp Val Val Cys Gln Ala Thr Pro Gly Phe

180 185 190

Ile Val Asn Arg Met Ala Arg Pro Tyr Tyr Leu Glu Gly Phe Arg Leu

195 200 205

Leu Glu Glu His Val Ala Arg Ala Pro Gln Ile Asp Arg Ala Leu Lys

210 215 220

Ala Gly Gly His Phe Arg Met Gly Pro Leu Glu Leu Thr Asp Phe Ile

225 230 235 240

Gly Gln Asp Ile Asn Tyr Gln Val Ser Arg Gln Ile Trp Gln Asp Met

245 250 255

Gln Tyr Asp Pro Arg Tyr Thr Pro Gly His Leu Gln Arg Ser Leu Val

260 265 270

Asp Ala Gly Leu Leu Gly Lys Lys Asn Gly Arg Ser Tyr Phe Ser Ala

275 280 285

Glu Glu Ser Pro Pro Pro Leu Ala Ala Ala Val Asp Ala Glu Val Glu

290 295 300

Thr Leu Arg Ile Tyr Gly Glu His Pro Leu Phe Thr Leu Leu Gln Gln

305 310 315 320

Arg Ala Ala Leu Gln Trp Pro Arg Leu Arg Val Glu Gln Arg Pro Thr

325 330 335

Leu Pro Gly Leu Gly Ala Ala Ile Gln Val Asn Asp Ala Phe Thr Val

340 345 350

Ser Val Thr Asp Gly Arg Thr Ala Asn Gln Leu Ala Glu Gln Thr Ala

355 360 365

Ala Asp Ala Phe Val Val Asp Val Ala Leu Asn Tyr Gly Asp Thr Ala

370 375 380

Tyr Leu Val Ala Ala His Ser Arg His Ala Ser Ala Ala Asn Lys Ala

385 390 395 400

Leu Phe Leu Arg Leu Leu His Thr Ala Ile Pro Gln Val Glu Phe Ile

405 410 415

Lys Asp Ser Pro Ala Leu Ile Val Ala Arg Val Leu Ser Ser Leu Ile

420 425 430

Asn Glu Ser Val Ile Met Val Glu Ser Gly Val Cys Ser Arg Glu Asp

435 440 445

Ile Asp Val Ala Ala Val Ala Gly Val Asn Tyr Ala Asp Gly Ile Phe

450 455 460

Gly Trp Leu Thr Arg Leu Gly Glu Glu Asn Val Arg Thr Thr Leu Ser

465 470 475 480

Asn Leu Ala Gln Leu Leu His Ala Ala Arg Tyr Ala Pro His Tyr Thr

485 490 495

Leu Leu His Ala Ala Gln Pro Ala Leu Thr Thr Thr Pro

500 505

<210> 15

<211> 509

<212> PRT

<213> Serratia sp. SCBI

<400> 15

Met Ala Glu Ser Asn Ala Ala Ile Gln Ser Ala Ala Ile Ile Gly Ala

1 5 10 15

Gly Thr Met Gly Arg Gly Ile Ala Tyr Leu Leu Ala Gln Lys Ser Ile

20 25 30

Arg Thr Val Leu Tyr Asn Arg Asn Gly Asn Thr Leu Asn Gln Ala Arg

35 40 45

Asp Ala Ile Val Gln Asp Leu Asn Lys Lys Val Glu Gln Gly Lys Leu

50 55 60

Ala Leu Gln Asp Lys Asp Ala Val Leu Ala Asn Leu Thr Phe Ser Ala

65 70 75 80

Glu Phe Gly Ala Ile Ala Asp Ser Asp Leu Val Ile Glu Thr Ile Ala

85 90 95

Glu His Glu Gln Ala Lys Leu Glu Val Leu Ala Ala Ile Ala Ala Thr

100 105 110

Val Lys Pro Asp Thr Leu Ile Ala Thr Asn Thr Ser Ser Leu Ser Leu

115 120 125

Asn Lys Leu Ala Thr Ala Val Thr His Ser Glu Arg Phe Ile Gly Leu

130 135 140

His Phe Phe Asn Pro Ala Pro Leu Met Lys Leu Ile Glu Ile Ile Pro

145 150 155 160

Ala Tyr Phe Thr Ala Gln Val Thr Thr Glu Arg Cys Arg Gln Leu Val

165 170 175

Ala Ala Leu Gly Lys Arg Asp Val Val Cys Gln Ala Thr Pro Gly Phe

180 185 190

Ile Val Asn Arg Met Ala Arg Pro Tyr Tyr Leu Glu Gly Phe Arg Leu

195 200 205

Leu Glu Glu His Val Ala Arg Ala Pro Gln Ile Asp Arg Ala Leu Lys

210 215 220

Ala Gly Gly His Phe Arg Met Gly Pro Leu Glu Leu Thr Asp Phe Ile

225 230 235 240

Gly Gln Asp Ile Asn Tyr Gln Val Ser Arg Gln Ile Trp Gln Asp Met

245 250 255

Gln Tyr Asp Pro Arg Tyr Thr Pro Gly His Leu Gln Arg Ser Leu Val

260 265 270

Asp Ala Gly Leu Leu Gly Lys Lys Asn Gly Arg Ser Tyr Phe Ser Thr

275 280 285

Glu Glu Ser Pro Pro Pro Leu Ala Ala Ala Val Asp Ala Glu Val Glu

290 295 300

Thr Leu Arg Ile Tyr Gly Glu His Pro Leu Phe Thr Leu Leu Gln Gln

305 310 315 320

Arg Ala Ala Leu Gln Trp Pro Arg Leu Arg Val Glu Gln Arg Pro Thr

325 330 335

Leu Pro Gly Leu Gly Ala Ala Ile Gln Val Asn Asp Ala Phe Thr Val

340 345 350

Ser Val Thr Asp Gly Arg Thr Ala Asn Gln Leu Ala Glu Gln Thr Ala

355 360 365

Ala Asp Ala Phe Val Val Asp Val Ala Leu Asn Tyr Gly Asp Thr Ala

370 375 380

Tyr Leu Val Ala Ala His Ser Arg His Ala Ser Ala Ala Asn Lys Ala

385 390 395 400

Leu Phe Leu Arg Leu Leu His Thr Ala Ile Pro Gln Val Glu Phe Ile

405 410 415

Lys Asp Ser Pro Ala Leu Ile Val Ala Arg Val Leu Ser Ser Leu Ile

420 425 430

Asn Glu Ser Val Ile Met Val Glu Ser Gly Val Cys Ser Arg Glu Asp

435 440 445

Ile Asp Val Ala Ala Val Ala Gly Val Asn Tyr Ala Asp Gly Ile Phe

450 455 460

Gly Trp Leu Thr Arg Leu Gly Glu Glu Asn Val Arg Thr Thr Leu Ser

465 470 475 480

Asn Leu Ala Gln Leu Leu His Ala Ala Arg Tyr Ala Pro His Tyr Thr

485 490 495

Leu Leu His Ala Ala Gln Pro Ala Leu Thr Thr Thr Pro

500 505

<210> 16

<211> 509

<212> PRT

<213> Serratia sp. S4

<400> 16

Met Ala Glu Asn Asn Ser Ala Ile His Ser Val Ala Val Ile Gly Ala

1 5 10 15

Gly Thr Met Gly Arg Gly Ile Ala Tyr Leu Leu Ala Gln Asn Gly Ile

20 25 30

Arg Thr Leu Leu Tyr Asn Arg Ser Gly Asn Asn Leu Asn Gln Ala Arg

35 40 45

Asp Tyr Ile Ile Arg Asp Leu Asp Lys Lys Ile Asp Gly Gly Lys Ile

50 55 60

Ser Leu Gln Lys Lys Gly Glu Ile Leu Ala Asn Leu Val Phe Ser Pro

65 70 75 80

Ile Phe Glu Ala Ile Ala Asp Ser Asp Leu Val Ile Glu Thr Ile Ala

85 90 95

Glu His Glu Ser Thr Lys His Glu Ile Leu Ser Ala Ile Ala Ala Thr

100 105 110

Val Lys Lys Glu Ala Ile Ile Ala Thr Asn Thr Ser Ser Leu Ser Leu

115 120 125

Asn Lys Leu Ala Ala Gly Val Glu Asn Asn Ala Arg Phe Ile Gly Leu

130 135 140

His Phe Phe Asn Pro Ala Pro Leu Met Lys Leu Ile Glu Ile Ile Pro

145 150 155 160

Ser Tyr Phe Thr Ser Arg Ala Thr Ser Leu Arg Cys Gln Gln Leu Val

165 170 175

Thr Val Ile Gly Lys Gln Phe Val Val Cys Lys Ala Thr Pro Gly Phe

180 185 190

Ile Val Asn Arg Met Ala Arg Pro Phe Tyr Leu Glu Gly Phe Arg Leu

195 200 205

Leu Glu Glu Asn Val Ala Leu Ala Pro Gln Ile Asp Arg Ala Leu Lys

210 215 220

Ala Gly Gly His Phe Arg Met Gly Pro Leu Glu Leu Thr Asp Phe Ile

225 230 235 240

Gly Gln Asp Ile Asn Tyr Gln Val Ser Ser Gln Ile Trp Gln Asp Met

245 250 255

Gln Tyr Asp Pro Arg Tyr Thr Pro Gly His Leu Gln Arg Ser Leu Val

260 265 270

Asp Ala Gly Leu Leu Gly Lys Lys Asn Gly Arg Ser Phe Phe Thr Pro

275 280 285

Ser Ser Ala Thr Pro Ser Ser Ala Asp Ala Gly Ser Gly Thr Pro Thr

290 295 300

Ser Leu Asn Phe Tyr Gly Glu His Pro Leu Phe Asp Leu Leu Gln Gln

305 310 315 320

Arg Ala Leu Ala Leu Trp Pro Arg Leu Gln Ile Asn Arg Gln Ser Glu

325 330 335

Gln Pro Thr Leu Gly Arg Phe Ile Arg Val Asn Asp Ala Met Ala Ile

340 345 350

Lys Ile Thr Asp Gly Arg Thr Ala Asn Leu Leu Ala Glu Leu Thr Glu

355 360 365

Leu Asp Thr Phe Val Ile Asp Ala Ala Leu Asn Tyr Ala Asp Thr Ala

370 375 380

Tyr Leu Ala Ala Ala His Ser Gln Asp Ala Ser Thr Ala Asn Lys Ala

385 390 395 400

Leu Phe Leu Thr Leu Leu Gln Thr Leu Ile Pro Gln Val Glu Phe Ile

405 410 415

Lys Asp Ser Pro Gly Leu Ile Val Ala Arg Val Leu Ser Ser Leu Ile

420 425 430

Asn Glu Ser Val Ile Met Val Glu Ser Gly Val Cys Ser Arg Ala Asp

435 440 445

Ile Asp Ile Ala Ala Val Ala Gly Val Asn Tyr Ala Asp Gly Ile Phe

450 455 460

Ser Trp Leu Ala Gln Leu Gly Gln Lys Asn Val Lys Ser Thr Leu Asp

465 470 475 480

Asn Met Ala Gln Leu Leu His Ser Ala Arg Tyr Tyr Pro His Tyr Ser

485 490 495

Leu Leu Asn Thr Pro Arg Pro Glu Leu Ala Val Ala Pro

500 505

<210> 17

<211> 509

<212> PRT

<213> Serratia sp. C-1

<400> 17

Met Ala Glu Asn Asn Ser Ala Ile Arg Ser Ala Ala Val Ile Gly Ala

1 5 10 15

Gly Thr Met Gly Arg Gly Ile Ala Tyr Leu Leu Ala Leu Asn Gly Ile

20 25 30

Arg Thr Val Leu Tyr Asn Arg Asn Gly Asn Asn Leu Asn Gln Ala Arg

35 40 45

Asp Tyr Ile Val Ser Asp Leu Asp Arg Lys Ile Asp Asn Gly Lys Ile

50 55 60

Thr Leu Gln Lys Lys Gly Gln Ile Leu Ala Asn Ile Ile Phe Ser Ala

65 70 75 80

Val Phe Asp Ala Ile Thr Asp Ser Asp Leu Val Ile Glu Thr Ile Ala

85 90 95

Glu Asp Glu Gln Thr Lys His Glu Ile Leu Ala Ala Ile Ala Ala Thr

100 105 110

Val Lys Pro Glu Ala Ile Ile Ala Thr Asn Thr Ser Ser Leu Ser Leu

115 120 125

Asn Lys Leu Ala Ala Gly Val Glu Asn Asn Pro Arg Phe Ile Gly Leu

130 135 140

His Phe Phe Asn Pro Ala Pro Leu Met Lys Leu Ile Glu Ile Ile Pro

145 150 155 160

Ser Tyr Phe Thr Ser His Ala Thr Ser Leu Arg Cys Gln Lys Leu Val

165 170 175

Ile Ala Leu Gly Lys Gln Phe Val Val Cys Lys Ala Thr Pro Gly Phe

180 185 190

Ile Val Asn Arg Met Ala Arg Pro Phe Tyr Leu Glu Gly Phe Arg Leu

195 200 205

Leu Glu Glu Asn Val Ala Leu Ala Pro Gln Ile Asp Arg Ala Leu Lys

210 215 220

Ala Gly Gly His Phe Arg Met Gly Pro Leu Glu Leu Thr Asp Phe Ile

225 230 235 240

Gly Gln Asp Ile Asn Tyr Gln Val Ser Lys Gln Ile Trp Gln Asp Met

245 250 255

Gln Phe Asp Pro Arg Tyr Thr Pro Gly His Leu Gln Arg Ser Leu Val

260 265 270

Asp Ala Gly Leu Leu Gly Arg Lys Asn Gly Arg Ser Phe Phe Ala Ser

275 280 285

Gln Pro Ala Thr Pro Pro Thr Pro Thr Thr Glu Ser Asp Thr Pro Thr

290 295 300

Ser Leu His Phe Tyr Gly Glu His Ala Leu Phe Asp His Leu Gln Gln

305 310 315 320

Arg Ala Leu Ala Ala Trp Pro Ala Leu Arg Val Gln Arg Leu Pro Glu

325 330 335

Arg Pro Glu Leu Gly Arg Phe Ile Leu Val Asn Asn Ala Leu Ala Ile

340 345 350

Lys Ile Thr Asp Gly Arg Thr Ala Asn Leu Leu Ala Gly Leu Thr Ala

355 360 365

Leu Asp Thr Phe Val Ile Asp Ala Ala Leu Asn Tyr Ala Asp Thr Ala

370 375 380

Tyr Leu Val Ala Ala His Asn Gln His Ala Thr Glu Thr Asn Lys Ala

385 390 395 400

Leu Phe Leu Thr Leu Leu Gln Thr Val Ile Ala Gln Val Glu Phe Ile

405 410 415

Lys Asp Ser Pro Ala Leu Ile Val Ala Arg Val Leu Ser Ser Leu Ile

420 425 430

Asn Glu Ser Val Ile Met Val Glu Ser Gly Val Cys Ser Arg Ala Asp

435 440 445

Ile Asp Ile Ala Ala Val Ala Gly Val Asn Tyr Ala Asp Gly Ile Phe

450 455 460

Ala Trp Leu Ala Gln Leu Gly Gln Lys Asn Val Lys Ser Thr Leu Asp

465 470 475 480

Asn Met Ala Gln Leu Leu His Ser Ala Arg Tyr Tyr Pro His Tyr Ser

485 490 495

Leu Leu Asn Ala Ala Arg Pro Glu Leu Ala Val Ala Pro

500 505

<210> 18

<211> 509

<212> PRT

<213> Serratia marcescens 532

<400> 18

Met Ala Glu Ser Asn Ala Ala Ile Gln Ser Ala Ala Ile Ile Gly Ala

1 5 10 15

Gly Thr Met Gly Arg Gly Ile Ala Tyr Leu Phe Ala Gln Lys Gly Ile

20 25 30

Arg Thr Val Leu Tyr Asn Arg Asn Gly Asn Thr Leu Asn Gln Ala Arg

35 40 45

Glu Tyr Ile Ala Gln Asp Leu Asn Lys Lys Val Glu Gln Gly Lys Ile

50 55 60

Ala Leu Gln Asp Lys Gly Ala Val Leu Ala Asn Leu Met Phe Thr Ser

65 70 75 80

Val Phe Glu Ala Ile Thr Asp Ser Glu Leu Val Ile Glu Thr Ile Ala

85 90 95

Glu Gln Glu Gln Thr Lys Leu Glu Val Leu Ala Ala Ile Ala Ala Val

100 105 110

Val Lys Pro Asp Thr Leu Ile Ala Thr Asn Thr Ser Ser Leu Ser Leu

115 120 125

Asn Lys Leu Ala Thr Ala Val Thr His Ser Glu Arg Phe Ile Gly Leu

130 135 140

His Phe Phe Asn Pro Ala Pro Leu Met Lys Leu Ile Glu Ile Ile Pro

145 150 155 160

Ala Tyr Phe Thr Ala His Ala Thr Thr Glu Arg Cys Arg Gln Leu Val

165 170 175

Ala Ala Leu Gly Lys His Asp Val Val Cys Gln Ala Thr Pro Gly Phe

180 185 190

Ile Val Asn Arg Met Ala Arg Pro Tyr Tyr Leu Glu Gly Phe Arg Leu

195 200 205

Leu Glu Glu His Val Ala Arg Ala Ala Gln Ile Asp Arg Ala Leu Lys

210 215 220

Ala Gly Gly Arg Phe Arg Met Gly Pro Leu Glu Leu Thr Asp Phe Ile

225 230 235 240

Gly Gln Asp Ile Asn Tyr Gln Val Ser Arg Gln Ile Trp Gln Asp Met

245 250 255

Gln Tyr Asp Pro Arg Tyr Thr Pro Gly His Leu Gln Arg Ser Leu Val

260 265 270

Asp Ala Gly Leu Leu Gly Lys Lys Asn Gly Arg Ser Tyr Phe Ala Ala

275 280 285

Glu Glu Thr Ala Pro Pro Val Thr Ala Ala Ser Asn Ala Asp Val Glu

290 295 300

Thr Leu Arg Val Tyr Gly Glu His Pro Phe Phe Thr Leu Leu Gln Gln

305 310 315 320

Arg Ala Ala Leu Gln Trp Pro Gln Leu Arg Val Glu Gln Arg Pro Ala

325 330 335

Leu Pro Gly Leu Gly Ser Ala Val Gln Ile Asn Asp Ala Phe Thr Val

340 345 350

Ser Ile Thr Asp Gly Arg Thr Ala Ser Gln Leu Ala Glu Gln Thr Ala

355 360 365

Ala Asp Ala Phe Val Val Asp Val Ala Leu Asn Tyr Ala Asp Thr Thr

370 375 380

Tyr Leu Ala Ala Ala His Ser Arg His Ala Ser Ala Ala Asn Lys Ala

385 390 395 400

Leu Phe Leu Arg Leu Leu His Thr Ala Ile Pro Gln Val Glu Phe Ile

405 410 415

Lys Asp Ser Pro Ala Leu Ile Val Ala Arg Val Leu Ser Ser Leu Ile

420 425 430

Asn Glu Ser Val Ile Met Val Glu Ser Gly Val Cys Ser Arg Glu Asp

435 440 445

Ile Asp Val Ala Ala Val Ala Gly Val Asn Tyr Ala Gly Gly Ile Phe

450 455 460

Asp Trp Leu Gly Lys Leu Gly Glu Lys Asn Val Arg Thr Thr Leu Ser

465 470 475 480

Asn Leu Ala Gln Leu Leu His Ala Ala Arg Tyr Ala Pro His Tyr Thr

485 490 495

Leu Leu His Ala Ala Gln Pro Ala Leu Thr Thr Thr Pro

500 505

<210> 19

<211> 509

<212> PRT

<213> Serratia marcescens 2880STDY5683033

<400> 19

Met Ala Glu Ser Asn Ala Ala Ile Gln Ser Ala Ala Ile Ile Gly Ala

1 5 10 15

Gly Thr Met Gly Arg Gly Ile Ala Tyr Leu Phe Ala Gln Lys Gly Ile

20 25 30

Arg Thr Val Leu Tyr Asn Arg Asn Gly Asn Thr Leu Asn Gln Ala Arg

35 40 45

Glu Tyr Ile Ala His Asp Leu Asn Lys Lys Val Glu Gln Gly Lys Ile

50 55 60

Ala Leu Gln Asp Lys Gly Ala Val Leu Ala Asn Leu Met Phe Thr Ser

65 70 75 80

Val Phe Glu Ala Ile Ala Asp Ser Glu Leu Val Ile Glu Thr Ile Ala

85 90 95

Glu Gln Glu Gln Thr Lys Leu Glu Val Leu Ala Ala Ile Ala Ala Val

100 105 110

Val Lys Pro Asp Thr Leu Ile Ala Thr Asn Thr Ser Ser Leu Ser Leu

115 120 125

Asn Lys Leu Ala Thr Ala Val Thr His Ser Glu Arg Phe Ile Gly Leu

130 135 140

His Phe Phe Asn Pro Ala Pro Leu Met Lys Leu Ile Glu Ile Ile Pro

145 150 155 160

Ala Tyr Phe Thr Ala His Ala Thr Thr Glu Arg Cys Arg Gln Leu Val

165 170 175

Ala Ala Leu Gly Lys His Asp Val Val Cys Gln Ala Thr Pro Gly Phe

180 185 190

Ile Val Asn Arg Met Ala Arg Pro Tyr Tyr Leu Glu Gly Phe Arg Leu

195 200 205

Leu Glu Glu His Val Ala Arg Ala Ala Gln Ile Asp Arg Ala Leu Lys

210 215 220

Ala Gly Gly Arg Phe Arg Met Gly Pro Leu Glu Leu Thr Asp Phe Ile

225 230 235 240

Gly Gln Asp Ile Asn Tyr Gln Val Ser Arg Gln Ile Trp Gln Asp Met

245 250 255

Gln Tyr Asp Pro Arg Tyr Thr Pro Gly His Leu Gln Arg Ser Leu Val

260 265 270

Asp Ala Gly Leu Leu Gly Lys Lys Asn Gly Arg Ser Tyr Phe Ala Thr

275 280 285

Glu Glu Thr Ala Pro Pro Val Thr Ala Ala Ser Asn Ala Asp Val Glu

290 295 300

Thr Leu Arg Val Tyr Gly Glu His Pro Phe Phe Thr Leu Leu Gln Gln

305 310 315 320

Arg Ala Ala Leu Gln Trp Pro Gln Leu Arg Val Glu Gln Arg Pro Ala

325 330 335

Leu Pro Gly Leu Gly Ser Ala Val Gln Ile Asn Asp Ala Phe Thr Val

340 345 350

Ser Ile Thr Asp Gly Arg Thr Ala Ser Gln Leu Ala Glu Gln Thr Ala

355 360 365

Ala Asp Ala Phe Val Val Asp Val Ala Leu Asn Tyr Ala Asp Thr Thr

370 375 380

Tyr Leu Ala Ala Ala His Ser Arg His Ala Ser Ala Ala Asn Lys Ala

385 390 395 400

Leu Phe Leu Arg Leu Leu His Thr Ala Ile Pro Gln Val Glu Phe Ile

405 410 415

Lys Asp Ser Pro Ala Leu Ile Val Ala Arg Val Leu Ser Ser Leu Ile

420 425 430

Asn Glu Ser Val Ile Met Val Glu Ser Gly Val Cys Ser Arg Glu Asp

435 440 445

Ile Asp Val Ala Ala Val Ala Gly Val Asn Tyr Ala Gly Gly Ile Phe

450 455 460

Asp Trp Leu Gly Lys Leu Gly Glu Lys Asn Val Arg Thr Thr Leu Ser

465 470 475 480

Asn Leu Ala Gln Leu Leu His Ala Ala Arg Tyr Ala Pro His Tyr Thr

485 490 495

Leu Leu His Ala Ala Gln Pro Ala Leu Thr Thr Thr Pro

500 505

<210> 20

<211> 509

<212> PRT

<213> Serratia marcescens WW4

<400> 20

Met Ala Glu Ser Asn Ala Ala Ile Gln Ser Ala Ala Ile Ile Gly Ala

1 5 10 15

Gly Thr Met Gly Arg Gly Ile Ala Tyr Leu Phe Ala Gln Lys Gly Ile

20 25 30

Pro Thr Met Leu Tyr Asn Arg Asn Gly Asn Thr Leu Asn Gln Ala Arg

35 40 45

Glu Tyr Ile Ala Gln Asp Leu Asn Lys Lys Val Glu Gln Gly Lys Ile

50 55 60

Ala Leu Gln Asp Lys Asp Ala Val Leu Ala Asn Leu Met Phe Thr Ser

65 70 75 80

Val Phe Glu Ala Ile Ala Asp Ser Glu Leu Val Ile Glu Thr Ile Ala

85 90 95

Glu Gln Glu Gln Thr Lys Leu Glu Val Leu Ala Ala Ile Ala Ala Val

100 105 110

Val Lys Pro Asp Thr Leu Ile Ala Thr Asn Thr Ser Ser Leu Ser Leu

115 120 125

Asn Lys Leu Ala Thr Ala Val Thr His Ser Glu Arg Phe Ile Gly Leu

130 135 140

His Phe Phe Asn Pro Ala Pro Leu Met Lys Leu Ile Glu Ile Ile Pro

145 150 155 160

Ala Tyr Phe Thr Ala His Ala Thr Thr Glu Arg Cys Arg Gln Leu Val

165 170 175

Ala Ala Leu Gly Lys Arg Asp Val Val Cys Gln Ala Thr Pro Gly Phe

180 185 190

Ile Val Asn Arg Met Ala Arg Pro Tyr Tyr Leu Glu Gly Phe Arg Leu

195 200 205

Leu Glu Glu His Val Ala Arg Ala Ala Gln Ile Asp Arg Ala Leu Lys

210 215 220

Ala Gly Gly Arg Phe Arg Met Gly Pro Leu Glu Leu Thr Asp Phe Ile

225 230 235 240

Gly Gln Asp Ile Asn Tyr Gln Val Ser Arg Gln Ile Trp Gln Asp Met

245 250 255

Gln Tyr Asp Pro Arg Tyr Thr Pro Gly His Leu Gln Arg Ser Leu Val

260 265 270

Asp Ala Gly Leu Leu Gly Lys Lys Asn Gly Arg Ser Tyr Phe Ala Ala

275 280 285

Glu Glu Thr Ala Pro Pro Val Thr Ala Ala Asn Asn Ala Asp Val Glu

290 295 300

Thr Leu Arg Val Tyr Gly Glu His Pro Phe Phe Thr Leu Leu Gln Gln

305 310 315 320

Arg Ala Ala Leu Gln Trp Pro Gln Leu Arg Val Glu Gln Arg Pro Ala

325 330 335

Leu Pro Gly Leu Gly Ala Ala Val Gln Ile Asn Asp Ala Phe Thr Val

340 345 350

Ser Ile Thr Asp Gly Arg Thr Ala Ser Gln Leu Ala Glu Gln Thr Ala

355 360 365

Ala Asp Ala Phe Val Ile Asp Val Ala Leu Asn Tyr Ala Asp Thr Thr

370 375 380

Tyr Leu Val Ala Ala His Ser Arg His Ala Ser Ala Ala Asn Lys Ala

385 390 395 400

Leu Phe Leu Arg Leu Leu His Thr Ala Ile Pro Gln Val Glu Phe Ile

405 410 415

Lys Asp Ser Pro Ala Leu Ile Val Ala Arg Val Leu Ser Ser Leu Ile

420 425 430

Asn Glu Ser Val Ile Met Val Glu Ser Gly Val Cys Ser Arg Glu Asp

435 440 445

Ile Asp Val Ala Ala Val Ala Gly Val Asn Tyr Ala Gly Gly Ile Phe

450 455 460

Asp Trp Leu Gly Lys Leu Gly Glu Lys Asn Val Arg Thr Thr Leu Ser

465 470 475 480

Asn Leu Ala Gln Leu Leu His Ala Ala Arg Tyr Ala Pro His Tyr Thr

485 490 495

Leu Leu His Ala Ala Gln Pro Ala Leu Thr Thr Thr Pro

500 505

<210> 21

<211> 509

<212> PRT

<213> Serratia marcescens K27

<400> 21

Met Ala Glu Ser Asn Ala Ala Ile Gln Ser Ala Ala Ile Ile Gly Ala

1 5 10 15

Gly Thr Met Gly Arg Gly Ile Ala Tyr Leu Phe Ala Gln Lys Gly Ile

20 25 30

Pro Thr Met Leu Tyr Asn Arg Asn Gly Asn Thr Leu Asn Gln Ala Arg

35 40 45

Glu Tyr Ile Ala Gln Asp Leu Asn Lys Lys Val Glu Gln Gly Lys Ile

50 55 60

Ala Leu Gln Asp Lys Asp Ala Val Leu Ala Asn Leu Met Phe Thr Ser

65 70 75 80

Val Phe Glu Ala Ile Ala Asp Ser Glu Leu Val Ile Glu Thr Ile Ala

85 90 95

Glu Gln Glu Gln Thr Lys Leu Glu Val Leu Ala Ala Ile Ala Ala Val

100 105 110

Val Lys Pro Asp Thr Leu Ile Ala Thr Asn Thr Ser Ser Leu Ser Leu

115 120 125

Asn Lys Leu Ala Thr Ala Val Thr His Ser Glu Arg Phe Ile Gly Leu

130 135 140

His Phe Phe Asn Pro Ala Pro Leu Met Lys Leu Ile Glu Ile Ile Pro

145 150 155 160

Ala Tyr Phe Thr Ala His Ala Thr Thr Glu Arg Cys Arg Gln Leu Val

165 170 175

Ala Ala Leu Gly Lys Arg Asp Val Val Cys Gln Ala Thr Pro Gly Phe

180 185 190

Ile Val Asn Arg Met Ala Arg Pro Tyr Tyr Leu Glu Gly Phe Arg Leu

195 200 205

Leu Glu Glu His Val Ala Arg Ser Ala Gln Ile Asp Arg Ala Leu Lys

210 215 220

Ala Gly Gly Arg Phe Arg Met Gly Pro Leu Glu Leu Thr Asp Phe Ile

225 230 235 240

Gly Gln Asp Ile Asn Tyr Gln Val Ser Arg Gln Ile Trp Gln Asp Met

245 250 255

Gln Tyr Asp Pro Arg Tyr Thr Pro Gly His Leu Gln Arg Ser Leu Val

260 265 270

Asp Ala Gly Leu Leu Gly Lys Lys Asn Gly Arg Ser Tyr Phe Ala Ala

275 280 285

Glu Glu Thr Ala Pro Pro Val Thr Ala Ala Asn Asn Ala Asp Val Glu

290 295 300

Thr Leu Arg Val Tyr Gly Glu His Pro Phe Phe Thr Leu Leu Gln Gln

305 310 315 320

Arg Ala Ala Leu Gln Trp Pro Gln Leu Arg Val Glu Gln Arg Pro Ala

325 330 335

Leu Pro Gly Leu Gly Ala Ala Val Gln Ile Asn Asp Ala Phe Thr Val

340 345 350

Ser Ile Thr Asp Gly Arg Thr Ala Ser Gln Leu Ala Glu Gln Thr Ala

355 360 365

Ala Asp Ala Phe Val Val Asp Val Ala Leu Asn Tyr Ala Asp Thr Thr

370 375 380

Tyr Leu Val Ala Ala His Ser Arg His Ala Ser Ala Ala Asn Lys Ala

385 390 395 400

Leu Phe Leu Arg Leu Leu His Thr Ala Ile Pro Gln Val Glu Phe Ile

405 410 415

Lys Asp Ser Pro Ala Leu Ile Val Ala Arg Val Leu Ser Ser Leu Ile

420 425 430

Asn Glu Ser Val Ile Met Val Glu Ser Gly Val Cys Ser Arg Glu Asp

435 440 445

Ile Asp Val Ala Ala Val Ala Gly Val Asn Tyr Ala Gly Gly Ile Phe

450 455 460

Asp Trp Leu Gly Lys Leu Gly Glu Arg Asn Val Arg Thr Thr Leu Ser

465 470 475 480

Asn Leu Ala Gln Leu Leu His Ala Ala Arg Tyr Ala Pro His Tyr Thr

485 490 495

Leu Leu His Ala Ala Gln Pro Ala Leu Thr Thr Thr Pro

500 505

<210> 22

<211> 509

<212> PRT

<213> Serratia marcescens 280

<400> 22

Met Ala Glu Ser Asn Ala Ala Ile Gln Ser Ala Ala Ile Ile Gly Ala

1 5 10 15

Gly Thr Met Gly Arg Gly Ile Ala Tyr Leu Phe Ala Gln Lys Gly Ile

20 25 30

Pro Thr Met Leu Tyr Asn Arg Asn Gly Asn Thr Leu Asn Gln Ala Arg

35 40 45

Glu Tyr Ile Ala Gln Asp Leu Asn Lys Lys Val Glu Gln Gly Lys Ile

50 55 60

Ala Leu Gln Asp Lys Asp Ala Val Leu Ala Asn Leu Met Phe Thr Ser

65 70 75 80

Val Phe Glu Ala Ile Ala Asp Ser Glu Leu Val Ile Glu Thr Ile Ala

85 90 95

Glu Gln Glu Gln Thr Lys Leu Glu Val Leu Ala Ala Ile Ala Ala Val

100 105 110

Val Lys Pro Asp Thr Leu Ile Ala Thr Asn Thr Ser Ser Leu Ser Leu

115 120 125

Asn Lys Leu Ala Thr Ala Val Thr His Ser Glu Arg Phe Ile Gly Leu

130 135 140

His Phe Phe Asn Pro Ala Pro Leu Met Lys Leu Ile Glu Ile Ile Pro

145 150 155 160

Ala Tyr Phe Thr Ala His Ala Thr Thr Glu Arg Cys Arg Gln Leu Val

165 170 175

Ala Ala Leu Gly Lys Arg Asp Val Val Cys Gln Ala Thr Pro Gly Phe

180 185 190

Ile Val Asn Arg Met Ala Arg Pro Tyr Tyr Leu Glu Gly Phe Arg Leu

195 200 205

Leu Glu Glu His Val Ala Arg Ala Ala Gln Ile Asp Arg Ala Leu Lys

210 215 220

Ala Gly Gly Arg Phe Arg Met Gly Pro Leu Glu Leu Thr Asp Phe Ile

225 230 235 240

Gly Gln Asp Ile Asn Tyr Gln Val Ser Arg Gln Ile Trp Gln Asp Met

245 250 255

Gln Tyr Asp Pro Arg Tyr Thr Pro Gly His Leu Gln Arg Ser Leu Val

260 265 270

Asp Ala Gly Leu Leu Gly Lys Lys Asn Gly Arg Ser Tyr Phe Ala Ala

275 280 285

Glu Glu Thr Ala Pro Pro Val Thr Ala Ala Asn Asn Ala Asp Val Glu

290 295 300

Thr Leu Arg Val Tyr Gly Glu His Pro Phe Phe Thr Leu Leu Gln Gln

305 310 315 320

Arg Ala Ala Leu Gln Trp Pro Gln Leu Arg Val Glu Gln Arg Pro Ala

325 330 335

Leu Pro Gly Leu Gly Ala Ala Val Gln Ile Asn Asp Ala Phe Thr Val

340 345 350

Ser Ile Thr Asp Gly Arg Thr Ala Ser Gln Leu Ala Glu Gln Thr Ala

355 360 365

Ala Asp Ala Phe Val Val Asp Val Ala Leu Asn Tyr Ala Asp Thr Thr

370 375 380

Tyr Leu Val Ala Ala His Ser Arg His Ala Ser Ala Ala Asn Lys Ala

385 390 395 400

Leu Phe Leu Leu Leu Leu His Thr Ala Ile Pro Gln Val Glu Phe Ile

405 410 415

Lys Asp Ser Pro Ala Leu Ile Val Ala Arg Val Leu Ser Ser Leu Ile

420 425 430

Asn Glu Ser Val Ile Met Val Glu Ser Gly Val Cys Ser Arg Glu Asp

435 440 445

Ile Asp Val Ala Ala Val Ala Gly Val Asn Tyr Ala Gly Gly Ile Phe

450 455 460

Asp Trp Leu Gly Lys Leu Gly Glu Lys Asn Val Arg Thr Thr Leu Ser

465 470 475 480

Asn Leu Ala Gln Leu Leu His Ala Ala Arg Tyr Ala Pro His Tyr Thr

485 490 495

Leu Leu His Ala Ala Gln Pro Ala Leu Thr Thr Thr Pro

500 505

<210> 23

<211> 509

<212> PRT

<213> Serratia marcescens 19F

<400> 23

Met Ala Glu Ser Asn Ala Ala Ile Gln Ser Ala Ala Ile Ile Gly Ala

1 5 10 15

Gly Thr Met Gly Arg Gly Ile Ala Tyr Leu Phe Ala Gln Lys Gly Ile

20 25 30

Pro Thr Met Leu Tyr Asn Arg Asn Gly Asn Thr Leu Asn Gln Ala Arg

35 40 45

Glu Tyr Ile Ala Gln Asp Leu Asn Lys Lys Val Glu Gln Gly Lys Ile

50 55 60

Ala Leu Gln Asp Lys Asp Ala Val Leu Ala Asn Leu Met Phe Thr Ser

65 70 75 80

Val Phe Glu Ala Ile Ala Asp Ser Glu Leu Val Ile Glu Thr Ile Ala

85 90 95

Glu Gln Glu Gln Thr Lys Leu Glu Val Leu Ala Ala Ile Ala Ala Val

100 105 110

Val Lys His Asp Thr Leu Ile Ala Thr Asn Thr Ser Ser Leu Ser Leu

115 120 125

Asn Lys Leu Ala Thr Ala Val Thr His Ser Glu Arg Phe Ile Gly Leu

130 135 140

His Phe Phe Asn Pro Ala Pro Leu Met Lys Leu Ile Glu Ile Ile Pro

145 150 155 160

Ala Tyr Phe Thr Ala His Ala Thr Thr Glu Arg Cys Arg Gln Leu Val

165 170 175

Ala Ala Leu Gly Lys Arg Asp Val Val Cys Gln Ala Thr Pro Gly Phe

180 185 190

Ile Val Asn Arg Met Ala Arg Pro Tyr Tyr Leu Glu Gly Phe Arg Leu

195 200 205

Leu Glu Glu His Val Ala Arg Ala Ala Gln Ile Asp Arg Ala Leu Lys

210 215 220

Ala Gly Gly Arg Phe Arg Met Gly Pro Leu Glu Leu Thr Asp Phe Ile

225 230 235 240

Gly Gln Asp Ile Asn Tyr Gln Val Ser Arg Gln Ile Trp Gln Asp Met

245 250 255

Gln Tyr Asp Pro Arg Tyr Thr Pro Gly His Leu Gln Arg Ser Leu Val

260 265 270

Asp Ala Gly Leu Leu Gly Lys Lys Asn Gly Arg Ser Tyr Phe Ala Ala

275 280 285

Glu Glu Thr Ala Pro Pro Val Thr Ala Ala Asn Asn Ala Asp Val Glu

290 295 300

Thr Leu Arg Val Tyr Gly Glu His Pro Phe Phe Thr Leu Leu Gln Gln

305 310 315 320

Arg Ala Ala Leu Gln Trp Pro Gln Leu Arg Val Glu Gln Arg Pro Ala

325 330 335

Leu Pro Gly Leu Gly Ala Ala Val Gln Ile Asn Asp Ala Phe Thr Val

340 345 350

Ser Ile Thr Asp Gly Arg Thr Ala Ser Gln Leu Ala Glu Gln Thr Ala

355 360 365

Ala Asp Ala Phe Val Val Asp Val Ala Leu Asn Tyr Ala Asp Thr Thr

370 375 380

Tyr Leu Val Ala Ala His Ser Arg His Ala Ser Ala Ala Asn Lys Ala

385 390 395 400

Leu Phe Leu Arg Leu Leu His Thr Ala Ile Pro Gln Val Glu Phe Ile

405 410 415

Lys Asp Ser Pro Ala Leu Ile Val Ala Arg Val Leu Ser Ser Leu Ile

420 425 430

Asn Glu Ser Val Ile Met Val Glu Ser Gly Val Cys Ser Arg Glu Asp

435 440 445

Ile Asp Val Ala Ala Val Ala Gly Val Asn Tyr Ala Gly Gly Ile Phe

450 455 460

Asp Trp Leu Gly Lys Leu Gly Glu Lys Asn Val Arg Thr Thr Leu Ser

465 470 475 480

Asn Leu Ala Gln Leu Leu His Ala Ala Arg Tyr Ala Pro His Tyr Thr

485 490 495

Leu Leu His Ala Ala Gln Pro Ala Leu Thr Thr Thr Pro

500 505

<210> 24

<211> 509

<212> PRT

<213> Serratia marcescens 1185

<400> 24

Met Ala Glu Ser Asn Ala Ala Ile Gln Ser Ala Ala Ile Ile Gly Ala

1 5 10 15

Gly Thr Met Gly Arg Gly Ile Ala Tyr Leu Phe Ala Gln Lys Gly Ile

20 25 30

Pro Thr Met Leu Tyr Asn Arg Asn Gly Asn Thr Leu Asn Gln Ala Arg

35 40 45

Glu Tyr Ile Ala Gln Asp Leu Asn Lys Lys Val Glu Gln Gly Lys Ile

50 55 60

Ala Leu Arg Asp Lys Asp Ala Val Leu Ala Asn Leu Met Phe Thr Ser

65 70 75 80

Val Phe Glu Ala Ile Ala Asp Ser Glu Leu Val Ile Glu Thr Ile Ala

85 90 95

Glu Gln Glu Gln Thr Lys Leu Glu Val Leu Ala Ala Ile Ala Ala Val

100 105 110

Val Lys Pro Asp Thr Leu Ile Ala Thr Asn Thr Ser Ser Leu Ser Leu

115 120 125

Asn Lys Leu Ala Thr Ala Val Thr His Ser Glu Arg Phe Ile Gly Leu

130 135 140

His Phe Phe Asn Pro Ala Pro Leu Met Lys Leu Ile Glu Ile Ile Pro

145 150 155 160

Ala Tyr Phe Thr Ala His Ala Thr Thr Glu Arg Cys Arg Gln Leu Val

165 170 175

Ala Ala Leu Gly Lys His Asp Val Val Cys Gln Ala Thr Pro Gly Phe

180 185 190

Ile Val Asn Arg Met Ala Arg Pro Tyr Tyr Leu Glu Gly Phe Arg Leu

195 200 205

Leu Glu Glu His Val Ala Arg Ala Ala Gln Ile Asp Arg Ala Leu Lys

210 215 220

Ala Gly Gly Arg Phe Arg Met Gly Pro Leu Glu Leu Thr Asp Phe Ile

225 230 235 240

Gly Gln Asp Ile Asn Tyr Gln Val Ser Arg Gln Ile Trp Gln Asp Met

245 250 255

Gln Tyr Asp Pro Arg Tyr Thr Pro Gly His Leu Gln Arg Ser Leu Val

260 265 270

Asp Ala Gly Leu Leu Gly Lys Lys Asn Gly Arg Ser Tyr Phe Ala Ala

275 280 285

Glu Glu Thr Ala Pro Pro Val Thr Ala Ala Asn Asn Ala Asp Val Glu

290 295 300

Thr Leu Arg Val Tyr Gly Glu His Pro Phe Phe Thr Leu Leu Gln Gln

305 310 315 320

Arg Ala Ala Leu Gln Trp Pro Gln Leu Arg Val Glu Gln Arg Pro Ala

325 330 335

Leu Pro Gly Leu Gly Ala Ala Val Gln Ile Asn Asp Ala Phe Thr Val

340 345 350

Ser Ile Thr Asp Gly Arg Thr Ala Ser Gln Leu Ala Glu Gln Thr Ala

355 360 365

Ala Asp Ala Phe Val Val Asp Leu Ala Leu Asn Tyr Ala Asp Thr Thr

370 375 380

Tyr Leu Val Ala Ala His Ser Arg His Ala Ser Ala Ala Asn Lys Ala

385 390 395 400

Leu Phe Leu Arg Leu Leu His Thr Ala Ile Pro Gln Val Glu Phe Ile

405 410 415

Lys Asp Ser Pro Ala Leu Ile Val Ala Arg Val Leu Ser Ser Leu Ile

420 425 430

Asn Glu Ser Val Ile Met Val Glu Ser Gly Val Cys Ser Arg Glu Asp

435 440 445

Ile Asp Val Ala Ala Val Ala Gly Val Asn Tyr Ala Gly Gly Ile Phe

450 455 460

Asp Trp Leu Gly Lys Leu Gly Glu Lys Asn Val Arg Thr Thr Leu Ser

465 470 475 480

Asn Leu Ala Gln Leu Leu His Ala Ala Arg Tyr Ala Pro His Tyr Thr

485 490 495

Leu Leu His Ala Ala Gln Ser Ala Leu Thr Thr Thr Pro

500 505

<210> 25

<211> 509

<212> PRT

<213> Serratia marcescens S2I7

<400> 25

Met Ala Glu Ser Asn Ala Ala Ile Gln Ser Ala Ala Ile Ile Gly Ala

1 5 10 15

Gly Thr Met Gly Arg Gly Ile Ala Tyr Leu Phe Ala Gln Lys Gly Ile

20 25 30

Pro Thr Met Leu Tyr Asn Arg Asn Gly Asn Thr Leu Asn Gln Ala Arg

35 40 45

Glu Tyr Ile Ala Gln Asp Leu Asn Lys Lys Val Glu Gln Gly Lys Ile

50 55 60

Ala Leu Gln Asp Lys Asp Ala Val Leu Ala Asn Leu Met Phe Thr Ser

65 70 75 80

Val Phe Glu Ala Ile Ala Asp Ser Glu Leu Val Ile Glu Thr Ile Ala

85 90 95

Glu Gln Glu Gln Thr Lys Leu Glu Val Leu Ala Ala Ile Ala Ala Val

100 105 110

Val Lys Pro Asp Thr Leu Ile Ala Thr Asn Thr Ser Ser Leu Ser Leu

115 120 125

Asn Lys Leu Ala Thr Ala Val Thr His Ser Glu Arg Phe Ile Gly Leu

130 135 140

His Phe Phe Asn Pro Ala Pro Leu Met Lys Leu Ile Glu Ile Ile Pro

145 150 155 160

Ala Tyr Phe Thr Ala His Ala Thr Thr Glu Arg Cys Arg Gln Leu Val

165 170 175

Ala Ala Leu Gly Lys Arg Asp Val Val Cys Gln Ala Thr Pro Gly Phe

180 185 190

Ile Val Asn Arg Met Ala Arg Pro Tyr Tyr Leu Glu Gly Phe Arg Leu

195 200 205

Leu Glu Glu His Val Ala Arg Ala Ala Gln Ile Asp Arg Ala Leu Lys

210 215 220

Ala Gly Gly Arg Phe Arg Met Gly Pro Leu Glu Leu Thr Asp Phe Ile

225 230 235 240

Gly Gln Asp Ile Asn Tyr Gln Val Ser Arg Gln Ile Trp Gln Asp Met

245 250 255

Gln Tyr Asp Pro Arg Tyr Thr Pro Gly His Leu Gln Arg Ser Leu Val

260 265 270

Asp Ala Gly Leu Leu Gly Lys Lys Asn Gly Arg Ser Tyr Phe Ala Ala

275 280 285

Glu Glu Thr Ala Pro Pro Val Thr Ala Ala Asn Asn Ala Asp Val Glu

290 295 300

Thr Leu Arg Val Tyr Gly Glu His Pro Phe Phe Thr Leu Leu Gln Gln

305 310 315 320

Arg Ala Ala Leu Gln Trp Pro Gln Leu Cys Val Glu Gln Arg Pro Ala

325 330 335

Leu Pro Gly Leu Gly Ala Ala Val Gln Ile Asn Asp Ala Phe Thr Val

340 345 350

Ser Ile Thr Asp Gly Arg Thr Ala Ser Gln Leu Ala Glu Gln Thr Ala

355 360 365

Ala Asp Ala Phe Val Val Asp Val Ala Leu Asn Tyr Ala Asp Thr Thr

370 375 380

Tyr Leu Val Ala Ala His Ser Arg His Ala Ser Ala Ala Asn Lys Ala

385 390 395 400

Leu Phe Leu Arg Leu Leu His Thr Ala Ile Pro Gln Val Glu Phe Ile

405 410 415

Lys Asp Ser Pro Ala Leu Ile Val Ala Arg Val Leu Ser Ser Leu Ile

420 425 430

Asn Glu Ser Val Ile Met Val Glu Ser Gly Val Cys Ser Arg Glu Asp

435 440 445

Ile Asp Val Ala Ala Val Ala Gly Val Asn Tyr Ala Gly Gly Ile Phe

450 455 460

Asp Trp Leu Gly Lys Leu Gly Glu Arg Asn Val Arg Thr Thr Leu Ser

465 470 475 480

Asn Leu Ala Gln Leu Leu His Ala Ala Arg Tyr Ala Pro His Tyr Thr

485 490 495

Leu Leu His Ala Ala Gln Pro Ala Leu Thr Thr Thr Pro

500 505

<210> 26

<211> 509

<212> PRT

<213> Serratia marcescens KHCo-24B

<400> 26

Met Ala Glu Ser Asn Ala Ala Ile Gln Ser Ala Ala Ile Ile Gly Ala

1 5 10 15

Gly Thr Met Gly Arg Gly Ile Ala Tyr Leu Phe Ala Gln Lys Gly Ile

20 25 30

Pro Thr Met Leu Tyr Asn Arg Asn Gly Asn Thr Leu Asn Gln Ala Arg

35 40 45

Glu Tyr Ile Ala Gln Asp Leu Asn Lys Lys Val Glu Gln Gly Lys Ile

50 55 60

Ala Leu Arg Asp Lys Asp Ala Val Leu Ala Asn Leu Met Phe Thr Ser

65 70 75 80

Val Phe Glu Ala Ile Ala Asp Ser Glu Leu Val Ile Glu Thr Ile Ala

85 90 95

Glu Gln Glu Gln Thr Lys Leu Glu Val Leu Ala Ala Ile Ala Ala Val

100 105 110

Val Lys Pro Asp Thr Leu Ile Ala Thr Asn Thr Ser Ser Leu Ser Leu

115 120 125

Asn Lys Leu Ala Thr Ala Val Thr His Ser Glu Arg Phe Ile Gly Leu

130 135 140

His Phe Phe Asn Pro Ala Pro Leu Met Lys Leu Ile Glu Ile Ile Pro

145 150 155 160

Ala Tyr Phe Thr Ala His Ala Thr Thr Glu Arg Cys Arg Gln Leu Val

165 170 175

Ala Ala Leu Gly Lys Arg Asp Val Val Cys Gln Ala Thr Pro Gly Phe

180 185 190

Ile Val Asn Arg Met Ala Arg Pro Tyr Tyr Leu Glu Gly Phe Arg Leu

195 200 205

Leu Glu Glu His Val Ala Arg Ala Ala Gln Ile Asp Arg Ala Leu Lys

210 215 220

Ala Gly Gly Arg Phe Arg Met Gly Pro Leu Glu Leu Thr Asp Phe Ile

225 230 235 240

Gly Gln Asp Ile Asn Tyr Gln Val Ser Arg Gln Ile Trp Gln Asp Met

245 250 255

Gln Tyr Asp Pro Arg Tyr Thr Pro Gly His Leu Gln Arg Ser Leu Val

260 265 270

Asp Ala Gly Leu Leu Gly Lys Lys Asn Gly Arg Ser Tyr Phe Ala Ala

275 280 285

Glu Glu Thr Ala Pro Pro Val Thr Ala Ala Asn Asn Ala Asp Val Glu

290 295 300

Thr Leu Arg Val Tyr Gly Glu His Pro Phe Phe Thr Leu Leu Gln Gln

305 310 315 320

Arg Ala Ala Leu Gln Trp Pro Gln Leu Arg Val Glu Gln Arg Pro Ala

325 330 335

Leu Pro Gly Leu Gly Ala Ala Val Gln Ile Asn Asp Ala Phe Thr Val

340 345 350

Ser Ile Thr Asp Gly Arg Thr Ala Ser Gln Leu Ala Glu Gln Ala Ala

355 360 365

Ala Asp Ala Phe Val Val Asp Leu Ala Leu Asn Tyr Ala Asp Thr Thr

370 375 380

Tyr Leu Val Ala Ala His Ser Arg His Ala Ser Ala Ala Asn Lys Ala

385 390 395 400

Leu Phe Leu Arg Leu Leu His Thr Ala Ile Pro Gln Val Glu Phe Ile

405 410 415

Lys Asp Ser Pro Ala Leu Ile Val Ala Arg Val Leu Ser Ser Leu Ile

420 425 430

Asn Glu Ser Val Ile Met Val Glu Ser Gly Val Cys Ser Arg Glu Asp

435 440 445

Ile Asp Val Ala Ala Val Ala Gly Val Asn Tyr Ala Gly Gly Ile Phe

450 455 460

Asp Trp Leu Gly Lys Leu Gly Glu Lys Asn Val Arg Thr Thr Leu Ser

465 470 475 480

Asn Leu Ala Gln Leu Leu His Ala Ala Arg Tyr Ala Pro His Tyr Thr

485 490 495

Leu Leu His Ala Ala Gln Pro Ala Leu Thr Thr Thr Pro

500 505

<210> 27

<211> 509

<212> PRT

<213> Serratia marcescens Z6

<400> 27

Met Ala Glu Ser Asn Ala Ala Ile Gln Ser Ala Ala Ile Ile Gly Ala

1 5 10 15

Gly Thr Met Gly Arg Gly Ile Ala Tyr Leu Phe Ala Gln Lys Gly Ile

20 25 30

Pro Thr Met Leu Tyr Asn Arg Asn Gly Asn Thr Leu Asn Gln Ala Arg

35 40 45

Glu Tyr Ile Ala Gln Asp Leu Asn Lys Lys Val Glu Gln Gly Lys Ile

50 55 60

Ala Leu Gln Asp Lys Asp Ala Val Leu Ala Asn Leu Met Phe Thr Ser

65 70 75 80

Val Phe Glu Ala Ile Ala Asp Ser Glu Leu Val Ile Glu Thr Ile Ala

85 90 95

Glu Gln Glu Gln Thr Lys Leu Glu Val Leu Ala Ala Ile Ala Ala Val

100 105 110

Val Lys Pro Asp Thr Leu Ile Ala Thr Asn Thr Ser Ser Leu Ser Leu

115 120 125

Asn Lys Leu Ala Thr Ala Val Thr His Arg Glu Arg Phe Ile Gly Leu

130 135 140

His Phe Phe Asn Pro Ala Pro Leu Met Lys Leu Ile Glu Ile Ile Pro

145 150 155 160

Ala Tyr Phe Thr Ala His Ala Thr Thr Glu Arg Cys Arg Gln Leu Val

165 170 175

Ala Ala Leu Gly Lys Arg Asp Val Val Cys Gln Ala Thr Pro Gly Phe

180 185 190

Ile Val Asn Arg Met Ala Arg Pro Tyr Tyr Leu Glu Gly Phe Arg Leu

195 200 205

Leu Glu Glu His Val Ala Arg Ala Ala Gln Ile Asp Arg Ala Leu Lys

210 215 220

Ala Gly Gly Arg Phe Arg Met Gly Pro Leu Glu Leu Thr Asp Phe Ile

225 230 235 240

Gly Gln Asp Ile Asn Tyr Gln Val Ser Arg Gln Ile Trp Gln Asp Met

245 250 255

Gln Tyr Asp Pro Arg Tyr Thr Pro Gly His Leu Gln Arg Ser Leu Val

260 265 270

Asp Ala Gly Leu Leu Gly Lys Lys Asn Gly Arg Ser Tyr Phe Ala Ala

275 280 285

Glu Glu Thr Ala Pro Pro Val Thr Ala Ala Asn Asn Ala Asp Val Glu

290 295 300

Thr Leu Arg Val Tyr Gly Glu His Pro Phe Phe Thr Leu Leu Gln Gln

305 310 315 320

Arg Ala Ala Leu Gln Trp Pro Gln Leu Cys Val Glu Gln Arg Pro Ala

325 330 335

Leu Pro Gly Leu Gly Ala Ala Val Gln Ile Asn Asp Ala Phe Thr Val

340 345 350

Ser Ile Thr Asp Gly Arg Thr Ala Ser Gln Leu Ala Glu Gln Thr Ala

355 360 365

Ala Asp Ala Phe Val Ile Asp Val Ala Leu Asn Tyr Ala Asp Thr Thr

370 375 380

Tyr Leu Val Ala Ala His Ser Arg His Ala Ser Ala Ala Asn Lys Ala

385 390 395 400

Leu Phe Leu Arg Leu Leu His Thr Ala Ile Pro Gln Val Glu Phe Ile

405 410 415

Lys Asp Ser Pro Ala Leu Ile Val Ala Arg Val Leu Ser Ser Leu Ile

420 425 430

Asn Glu Ser Val Ile Met Val Glu Ser Gly Val Cys Ser Arg Glu Asp

435 440 445

Ile Asp Val Ala Ala Val Ala Gly Val Asn Tyr Ala Gly Gly Ile Phe

450 455 460

Asp Trp Leu Gly Lys Leu Gly Glu Lys Asn Val Arg Thr Thr Leu Ser

465 470 475 480

Asn Leu Ala Gln Leu Leu His Ala Ala Arg Tyr Ala Pro His Tyr Thr

485 490 495

Leu Leu His Ala Ala Gln Pro Ala Leu Thr Thr Thr Pro

500 505

<210> 28

<211> 509

<212> PRT

<213> Serratia marcescens 546

<400> 28

Met Ala Glu Ser Asn Ala Ala Ile Gln Ser Ala Ala Ile Ile Gly Ala

1 5 10 15

Gly Thr Met Gly Arg Gly Ile Ala Tyr Leu Phe Ala Gln Lys Gly Ile

20 25 30

Pro Thr Met Leu Tyr Asn Arg Asn Gly Asn Thr Leu Asn Gln Ala Arg

35 40 45

Glu Tyr Ile Ala Gln Asp Leu Asn Lys Lys Val Glu Gln Gly Lys Ile

50 55 60

Ala Leu Arg Asp Lys Asp Ala Val Leu Ala Asn Leu Met Phe Thr Ser

65 70 75 80

Val Phe Glu Ala Ile Ala Asp Ser Glu Leu Val Ile Glu Thr Ile Ala

85 90 95

Glu Gln Glu Gln Thr Lys Leu Glu Val Leu Ala Ala Ile Ala Ala Val

100 105 110

Val Lys Pro Asp Thr Leu Ile Ala Thr Asn Thr Ser Ser Leu Ser Leu

115 120 125

Asn Lys Leu Ser Thr Ala Val Thr His Asn Glu Arg Phe Ile Gly Leu

130 135 140

His Phe Phe Asn Pro Ala Pro Leu Met Lys Leu Ile Glu Ile Ile Pro

145 150 155 160

Ala Tyr Phe Thr Ala His Ala Thr Thr Glu Arg Cys Arg Gln Leu Val

165 170 175

Ala Ala Leu Gly Lys Arg Asp Val Val Cys Gln Ala Thr Pro Gly Phe

180 185 190

Ile Val Asn Arg Met Ala Arg Pro Tyr Tyr Leu Glu Gly Phe Arg Leu

195 200 205

Leu Glu Glu His Val Ala Arg Ala Ala Gln Ile Asp Arg Ala Leu Lys

210 215 220

Ala Gly Gly Arg Phe Arg Met Gly Pro Leu Glu Leu Thr Asp Phe Ile

225 230 235 240

Gly Gln Asp Ile Asn Tyr Gln Val Ser Arg Gln Ile Trp Gln Asp Met

245 250 255

Gln Tyr Asp Pro Arg Tyr Thr Pro Gly His Leu Gln Arg Ser Leu Val

260 265 270

Asp Ala Gly Leu Leu Gly Lys Lys Asn Gly Arg Ser Tyr Phe Ala Ala

275 280 285

Glu Glu Thr Ala Pro Pro Val Thr Ala Ala Asn Asn Ala Asp Val Glu

290 295 300

Thr Leu Arg Val Tyr Gly Glu His Pro Phe Phe Thr Leu Leu Gln Gln

305 310 315 320

Arg Ala Ala Leu Gln Trp Pro Gln Leu Arg Val Glu Gln Arg Pro Ala

325 330 335

Leu Pro Gly Leu Gly Ala Ala Val Arg Ile Asn Asp Ala Phe Thr Val

340 345 350

Ser Ile Thr Asp Gly Arg Thr Ala Ser Gln Leu Ala Glu Gln Thr Ala

355 360 365

Ala Asp Ala Phe Val Val Asp Val Ala Leu Asn Tyr Ala Asp Thr Thr

370 375 380

Tyr Leu Val Ala Ala His Ser Arg His Ala Ser Ala Ala Asn Lys Ala

385 390 395 400

Leu Phe Leu Arg Leu Leu His Thr Ala Ile Pro Gln Val Glu Phe Ile

405 410 415

Lys Asp Ser Pro Ala Leu Ile Val Ala Arg Val Leu Ser Ser Leu Ile

420 425 430

Asn Glu Ser Val Ile Met Val Glu Ser Gly Val Cys Ser Arg Glu Asp

435 440 445

Ile Asp Val Ala Ala Val Ala Gly Val Asn Tyr Ala Gly Gly Ile Phe

450 455 460

Asp Trp Leu Gly Lys Leu Gly Glu Lys Asn Val Arg Thr Thr Leu Ser

465 470 475 480

Asn Leu Ala Gln Leu Leu His Ala Ala Arg Tyr Ala Pro His Tyr Thr

485 490 495

Leu Leu His Ala Ala Gln Pro Ala Leu Thr Thr Thr Pro

500 505

<210> 29

<211> 509

<212> PRT

<213> Serratia nematodiphila MB307

<400> 29

Met Ala Glu Ser Asn Ala Ala Ile Gln Ser Ala Ala Ile Ile Gly Ala

1 5 10 15

Gly Thr Met Gly Arg Gly Ile Ala Tyr Leu Phe Ala Gln Lys Gly Ile

20 25 30

Pro Thr Met Leu Tyr Asn Arg Asn Gly Asn Thr Leu Asn Gln Ala Arg

35 40 45

Glu Tyr Ile Ala Gln Asp Leu Asn Lys Lys Val Glu Gln Gly Lys Ile

50 55 60

Ala Leu Arg Asp Lys Asp Ala Val Leu Ala Asn Leu Met Phe Thr Ser

65 70 75 80

Val Phe Glu Ala Ile Ala Asp Ser Glu Leu Val Ile Glu Thr Ile Ala

85 90 95

Glu Gln Glu Gln Thr Lys Leu Glu Val Leu Ala Ala Ile Ala Ala Val

100 105 110

Val Lys Pro Asp Thr Leu Ile Ala Thr Asn Thr Ser Ser Leu Ser Leu

115 120 125

Asn Lys Leu Ala Thr Ala Val Thr His Ser Glu Arg Phe Ile Gly Leu

130 135 140

His Phe Phe Asn Pro Ala Pro Leu Met Lys Leu Ile Glu Ile Ile Pro

145 150 155 160

Ala Tyr Phe Thr Ala His Ala Thr Thr Glu Arg Cys Arg Gln Leu Val

165 170 175

Ala Ala Leu Gly Lys Arg Asp Val Val Cys Gln Ala Thr Pro Gly Phe

180 185 190

Ile Val Asn Arg Met Ala Arg Pro Tyr Tyr Leu Glu Gly Phe Arg Leu

195 200 205

Leu Glu Glu His Val Ala Arg Ala Ala Gln Ile Asp Arg Ala Leu Lys

210 215 220

Ala Gly Gly Arg Phe Arg Met Gly Pro Leu Glu Leu Thr Asp Phe Ile

225 230 235 240

Gly Gln Asp Ile Asn Tyr Gln Val Ser Arg Gln Ile Trp Gln Asp Met

245 250 255

Gln Tyr Asp Pro Arg Tyr Thr Pro Gly His Leu Gln Arg Ser Leu Val

260 265 270

Asp Ala Gly Leu Leu Gly Lys Lys Asn Gly Arg Ser Tyr Phe Ala Ala

275 280 285

Glu Glu Thr Ala Pro Gln Val Thr Ala Ala Asn Asn Ala Asp Val Glu

290 295 300

Thr Leu Arg Val Tyr Gly Glu His Pro Phe Phe Thr Leu Leu Gln Gln

305 310 315 320

Arg Ala Ala Leu Gln Trp Pro Gln Leu Arg Val Glu Gln Arg Pro Ala

325 330 335

Leu Pro Gly Leu Gly Ala Ala Val Gln Ile Asn Asp Ala Phe Thr Val

340 345 350

Ser Ile Thr Asp Gly Arg Thr Ala Ser Gln Leu Ala Glu Gln Thr Ala

355 360 365

Ala Asp Ala Phe Val Val Asp Leu Ala Leu Asn Tyr Ala Asp Thr Thr

370 375 380

Tyr Leu Val Ala Ala His Ser Arg His Ala Ser Ala Ala Asn Lys Ala

385 390 395 400

Leu Phe Leu Arg Leu Leu His Thr Ala Ile Pro Gln Val Glu Phe Ile

405 410 415

Lys Asp Ser Pro Ala Leu Ile Val Ala Arg Val Leu Ser Ser Leu Ile

420 425 430

Asn Glu Ser Val Ile Met Val Glu Ser Gly Val Cys Ser Arg Glu Asp

435 440 445

Ile Asp Val Ala Ala Val Ala Gly Val Asn Tyr Ala Gly Gly Ile Phe

450 455 460

Asp Trp Leu Gly Lys Leu Gly Glu Lys Asn Val Arg Thr Thr Leu Ser

465 470 475 480

Asn Leu Ala Gln Leu Leu His Ala Ala Arg Tyr Ala Pro His Tyr Thr

485 490 495

Leu Leu His Ala Ala Gln Pro Ala Leu Thr Thr Thr Pro

500 505

<210> 30

<211> 509

<212> PRT

<213> Serratia marcescens VGH107

<400> 30

Met Ala Glu Ser Asn Ala Ala Ile Gln Ser Ala Ala Ile Ile Gly Ala

1 5 10 15

Gly Thr Met Gly Arg Gly Ile Ala Tyr Leu Phe Ala Gln Lys Gly Ile

20 25 30

Pro Thr Met Leu Tyr Asn Arg Asn Gly Asn Thr Leu Asn Gln Ala Arg

35 40 45

Glu Tyr Ile Ala Gln Asp Leu Asn Lys Lys Val Glu Gln Gly Lys Ile

50 55 60

Ala Leu Gln Asp Lys Asp Ala Val Leu Ala Asn Leu Met Phe Thr Ser

65 70 75 80

Val Phe Glu Ala Ile Ala Asp Ser Glu Leu Val Ile Glu Thr Ile Ala

85 90 95

Glu Gln Glu Gln Thr Lys Leu Glu Val Leu Ala Ala Ile Ala Ala Val

100 105 110

Val Lys His Asp Thr Leu Ile Ala Thr Asn Thr Ser Ser Leu Ser Leu

115 120 125

Asn Lys Leu Ala Thr Ala Val Thr His Ser Glu Arg Phe Ile Gly Leu

130 135 140

His Phe Phe Asn Pro Ala Pro Leu Met Lys Leu Ile Glu Ile Ile Pro

145 150 155 160

Ala Tyr Phe Thr Ala His Ala Thr Thr Glu Arg Cys Arg Gln Leu Val

165 170 175

Ala Ala Leu Gly Lys Arg Asp Val Val Cys Gln Ala Thr Pro Gly Phe

180 185 190

Ile Val Asn Arg Met Ala Arg Pro Tyr Tyr Leu Glu Gly Phe Arg Leu

195 200 205

Leu Glu Glu His Val Ala Arg Ala Ala Gln Ile Asp Arg Ala Leu Lys

210 215 220

Ala Gly Gly Arg Phe Arg Met Gly Pro Leu Glu Leu Thr Asp Phe Ile

225 230 235 240

Gly Gln Asp Ile Asn Tyr Gln Val Ser Arg Gln Ile Trp Gln Asp Met

245 250 255

Gln Tyr Asp Pro Arg Tyr Thr Pro Gly His Leu Gln Arg Ser Leu Val

260 265 270

Asp Ala Gly Leu Leu Gly Lys Lys Asn Gly Arg Ser Tyr Phe Ala Ala

275 280 285

Glu Glu Thr Ala Pro Pro Val Thr Ala Ala Asn Asn Ala Asp Val Glu

290 295 300

Thr Leu Arg Val Tyr Gly Glu His Pro Phe Phe Thr Leu Leu Gln Gln

305 310 315 320

Arg Ala Ala Leu Gln Trp Pro Gln Leu Arg Val Glu Gln Arg Pro Ala

325 330 335

Leu Pro Gly Leu Gly Ala Ala Val Gln Ile Asn Asp Ala Phe Thr Val

340 345 350

Ser Ile Thr Asp Gly Arg Thr Ala Ser Gln Leu Ala Glu Gln Thr Ala

355 360 365

Ala Asp Ala Phe Val Val Asp Val Ala Leu Asn Tyr Ala Asp Thr Thr

370 375 380

Tyr Leu Val Ala Ala His Ser Arg His Ala Ser Ala Ala Asn Lys Ala

385 390 395 400

Leu Phe Leu Arg Leu Leu His Thr Ala Ile Pro Gln Val Glu Phe Ile

405 410 415

Lys Asp Ser Pro Ala Leu Ile Val Ala Arg Val Leu Ser Ser Leu Ile

420 425 430

Asn Glu Ser Val Ile Met Val Glu Ser Gly Val Cys Ser Arg Glu Asp

435 440 445

Ile Asp Val Ala Ala Val Ala Gly Val Asn Tyr Ala Gly Gly Ile Phe

450 455 460

Asp Trp Leu Gly Lys Leu Gly Glu Lys Asn Val Arg Thr Met Leu Ser

465 470 475 480

Asn Leu Ala Gln Leu Leu His Ala Ala Arg Tyr Ala Pro His Tyr Asn

485 490 495

Leu Leu His Ala Ala Gln Pro Ala Leu Thr Thr Thr Pro

500 505

<210> 31

<211> 509

<212> PRT

<213> Serratia marcescens MCB

<400> 31

Met Ala Glu Ser Asn Ala Ala Ile Gln Ser Ala Ala Ile Ile Gly Ala

1 5 10 15

Gly Thr Met Gly Arg Gly Ile Ala Tyr Leu Phe Ala Gln Lys Gly Ile

20 25 30

Arg Thr Val Leu Tyr Asn Arg Asn Gly Asn Thr Leu Asn Gln Ala Arg

35 40 45

Glu Tyr Ile Ala Gln Asp Leu Asn Lys Lys Val Glu Gln Gly Lys Ile

50 55 60

Ala Leu Gln Asp Lys Ser Ser Val Leu Ala Asn Leu Val Phe Ser Thr

65 70 75 80

Ala Leu Glu Ala Ile Ala Asp Ser Glu Leu Val Ile Glu Thr Ile Ala

85 90 95

Glu His Glu Gln Thr Lys Leu Glu Val Leu Ala Ala Ile Ala Ala Val

100 105 110

Val Lys Pro Asp Thr Leu Ile Ala Thr Asn Thr Ser Ser Leu Ser Leu

115 120 125

Asn Lys Leu Ala Thr Ala Val Thr His Ser Glu Arg Phe Ile Gly Leu

130 135 140

His Phe Phe Asn Pro Ala Pro Leu Met Lys Leu Ile Glu Ile Ile Pro

145 150 155 160

Ala Tyr Phe Thr Ala His Ala Thr Thr Glu Arg Cys Arg Gln Leu Val

165 170 175

Ala Ala Leu Gly Lys Arg Asp Val Val Cys Gln Ala Thr Pro Gly Phe

180 185 190

Ile Val Asn Arg Met Ala Arg Pro Tyr Tyr Leu Glu Gly Phe Arg Leu

195 200 205

Leu Glu Glu His Val Ala Arg Ala Pro Gln Ile Asp Arg Ala Leu Lys

210 215 220

Ala Gly Gly His Phe Arg Met Gly Pro Leu Glu Leu Thr Asp Phe Ile

225 230 235 240

Gly Gln Asp Ile Asn Tyr Gln Val Ser Arg Gln Ile Trp Gln Asp Met

245 250 255

Gln Tyr Asp Pro Arg Tyr Thr Pro Gly His Leu Gln Arg Ser Leu Val

260 265 270

Asp Ala Gly Leu Leu Gly Lys Lys Asn Gly Arg Ser Tyr Phe Thr Ala

275 280 285

Glu Glu Thr Ala Pro Pro Val Thr Ala Ala Ile Gly Ala Asp Val Glu

290 295 300

Thr Leu Arg Ile Tyr Gly Glu His Pro Phe Phe Thr Leu Leu Gln Gln

305 310 315 320

Arg Ala Ala Leu Gln Trp Pro Gln Leu Arg Val Glu Gln Arg Pro Ala

325 330 335

Leu Pro Gly Val Gly Pro Ala Val Gln Ile Asn Asp Ala Phe Thr Val

340 345 350

Ser Ile Thr Asp Gly Arg Thr Ala Ser Gln Leu Ala Glu Gln Thr Thr

355 360 365

Ala Asp Ala Phe Val Val Asp Ile Ala Leu Asn Tyr Ala Asp Thr Ala

370 375 380

Tyr Leu Val Ala Ala His Asn Arg His Ala Ser Ala Ala Asn Lys Ala

385 390 395 400

Leu Phe Leu Arg Leu Leu His Thr Ala Ile Pro Gln Val Glu Phe Ile

405 410 415

Lys Asp Ser Pro Ala Leu Ile Val Ala Arg Val Leu Ser Ser Leu Ile

420 425 430

Asn Glu Ser Val Ile Met Val Glu Ser Gly Val Cys Ser Arg Glu Asp

435 440 445

Ile Asp Val Ala Ala Val Ala Gly Val Asn Tyr Ala Gly Gly Ile Phe

450 455 460

Asp Trp Leu Ser Lys Leu Gly Glu Lys Asn Val Arg Thr Thr Leu Ser

465 470 475 480

Asn Leu Ala Gln Leu Leu His Ala Ala Arg Tyr Ala Pro His Tyr Thr

485 490 495

Leu Leu His Ala Ala Gln Pro Ala Leu Thr Thr Thr Pro

500 505

<210> 32

<211> 509

<212> PRT

<213> Serratia marcescens AH0650

<400> 32

Met Ala Glu Ser Asn Ala Ala Ile Gln Ser Ala Ala Ile Ile Gly Ala

1 5 10 15

Gly Thr Met Gly Arg Gly Ile Ala Tyr Leu Phe Ala Gln Lys Gly Ile

20 25 30

Arg Thr Val Leu Tyr Asn Arg Asn Gly Asn Thr Leu Asn Gln Ala Arg

35 40 45

Glu Tyr Ile Ala Gln Asp Leu Asn Lys Lys Val Glu Gln Gly Lys Ile

50 55 60

Ala Leu Gln Asp Lys Ser Ser Val Leu Ala Asn Leu Val Phe Ser Thr

65 70 75 80

Ala Leu Glu Ala Ile Ala Asp Ser Glu Leu Val Ile Glu Thr Ile Ala

85 90 95

Glu His Glu Gln Thr Lys Leu Glu Val Leu Ala Ala Ile Ala Ala Val

100 105 110

Val Lys Pro Asp Thr Leu Ile Ala Thr Asn Thr Ser Ser Leu Ser Leu

115 120 125

Asn Lys Leu Ala Thr Ala Val Thr His Ser Glu Arg Phe Ile Gly Leu

130 135 140

His Phe Phe Asn Pro Ala Pro Leu Met Lys Leu Ile Glu Ile Ile Pro

145 150 155 160

Ala Tyr Phe Thr Ala His Ala Thr Thr Glu Arg Cys Arg Gln Leu Val

165 170 175

Ala Ala Leu Gly Lys Arg Asp Val Val Cys Gln Ala Thr Pro Gly Phe

180 185 190

Ile Val Asn Arg Met Ala Arg Pro Tyr Tyr Leu Glu Gly Phe Arg Leu

195 200 205

Leu Glu Glu His Val Ala Arg Ala Pro Gln Ile Asp Arg Ala Leu Lys

210 215 220

Ala Gly Gly His Phe Arg Met Gly Pro Leu Glu Leu Thr Asp Phe Ile

225 230 235 240

Gly Gln Asp Ile Asn Tyr Gln Val Ser Arg Gln Ile Trp Gln Asp Met

245 250 255

Gln Tyr Asp Pro Arg Tyr Thr Pro Gly His Leu Gln Arg Ser Leu Val

260 265 270

Asp Ala Gly Leu Leu Gly Lys Lys Asn Gly Arg Ser Tyr Phe Ala Ala

275 280 285

Glu Glu Thr Ala Pro Pro Val Thr Ala Ala Ile Asp Ala Asp Val Glu

290 295 300

Thr Leu Arg Val Tyr Gly Glu His Pro Phe Phe Thr Leu Leu Gln Gln

305 310 315 320

Arg Ala Ala Arg Gln Trp Pro Gln Leu Arg Val Glu Gln Arg Pro Ala

325 330 335

Leu Pro Ser Leu Gly Pro Ala Val Gln Ile Asn Asp Ala Phe Thr Val

340 345 350

Ser Ile Thr Asp Gly Arg Thr Ala Ser Gln Leu Ala Glu Gln Thr Thr

355 360 365

Ala Asp Ala Phe Val Val Asp Ile Ala Leu Asn Tyr Ala Asp Thr Ala

370 375 380

Tyr Leu Val Ala Ala His Asn Arg Tyr Ala Ser Ala Ala Asn Lys Ala

385 390 395 400

Leu Phe Leu Arg Leu Leu His Thr Ala Ile Pro Gln Val Glu Phe Ile

405 410 415

Lys Asp Ser Pro Ala Leu Ile Val Ala Arg Val Leu Ser Ser Leu Ile

420 425 430

Asn Glu Ser Val Ile Met Val Glu Ser Gly Val Cys Ser Arg Glu Asp

435 440 445

Ile Asp Val Ala Ala Val Ala Gly Val Asn Tyr Ala Gly Gly Ile Phe

450 455 460

Asp Trp Leu Gly Lys Leu Gly Glu Lys Asn Val Arg Thr Thr Leu Ser

465 470 475 480

Asn Leu Ala Gln Leu Leu His Ala Ala Arg Tyr Ala Pro His Tyr Thr

485 490 495

Leu Leu His Ala Ala Gln Pro Ala Leu Thr Thr Thr Pro

500 505

<210> 33

<211> 509

<212> PRT

<213> Serratia marcescens UMH12

<400> 33

Met Ala Glu Ser Asn Ala Glu Ile Gln Ser Ala Ala Ile Ile Gly Ala

1 5 10 15

Gly Thr Met Gly Arg Gly Ile Ala Tyr Leu Phe Ala Gln Lys Gly Ile

20 25 30

Arg Thr Val Leu Tyr Asn Arg Asn Gly Asn Thr Leu Asn Gln Ala Arg

35 40 45

Glu Tyr Ile Ala Gln Asp Leu Asn Lys Lys Val Glu Gln Gly Lys Ile

50 55 60

Ala Leu Gln Asp Lys Ser Ser Val Leu Ala Asn Leu Val Phe Ser Thr

65 70 75 80

Ala Leu Glu Ala Ile Ala Asp Ser Glu Leu Val Ile Glu Thr Ile Ala

85 90 95

Glu His Glu Gln Thr Lys Leu Glu Val Leu Ala Ala Ile Ala Ala Val

100 105 110

Val Lys Pro Asp Thr Leu Ile Ala Thr Asn Thr Ser Ser Leu Ser Leu

115 120 125

Asn Lys Leu Ala Thr Ala Val Thr His Ser Glu Arg Phe Ile Gly Leu

130 135 140

His Phe Phe Asn Pro Ala Pro Leu Met Lys Leu Ile Glu Ile Ile Pro

145 150 155 160

Ala Tyr Phe Thr Ala His Ala Thr Thr Glu Arg Cys Arg Gln Leu Val

165 170 175

Ala Ala Leu Gly Lys Arg Asp Val Val Cys Gln Ala Thr Pro Gly Phe

180 185 190

Ile Val Asn Arg Met Ala Arg Pro Tyr Tyr Leu Glu Gly Phe Arg Leu

195 200 205

Leu Glu Glu His Val Ala Arg Ala Pro Gln Ile Asp Arg Ala Leu Lys

210 215 220

Ala Gly Gly His Phe Arg Met Gly Pro Leu Glu Leu Thr Asp Phe Ile

225 230 235 240

Gly Gln Asp Ile Asn Tyr Gln Val Ser Arg Gln Ile Trp Gln Asp Met

245 250 255

Gln Tyr Asp Pro Arg Tyr Thr Pro Gly His Leu Gln Arg Ser Leu Val

260 265 270

Asp Ala Gly Leu Leu Gly Lys Lys Asn Gly Arg Ser Tyr Phe Ala Ala

275 280 285

Glu Glu Thr Ala Pro Pro Val Thr Ala Ala Ile Asp Ala Asp Val Glu

290 295 300

Thr Leu Arg Val Tyr Gly Glu His Pro Phe Phe Thr Leu Leu Gln Gln

305 310 315 320

Arg Ala Ala Arg Gln Trp Pro Gln Leu Arg Val Glu Gln Arg Pro Ala

325 330 335

Leu Pro Ser Leu Gly Pro Ala Val Gln Ile Asn Asp Ala Phe Thr Val

340 345 350

Ser Ile Thr Asp Gly Arg Thr Ala Ser Gln Leu Ala Glu Gln Thr Thr

355 360 365

Ala Asp Ala Phe Val Val Asp Ile Ala Leu Asn Tyr Ala Asp Thr Ala

370 375 380

Tyr Leu Val Ala Ala His Asn Arg Tyr Ala Ser Ala Ala Asn Lys Ala

385 390 395 400

Leu Phe Leu Arg Leu Leu His Thr Ala Ile Pro Gln Val Glu Phe Ile

405 410 415

Lys Asp Ser Pro Ala Leu Ile Val Ala Arg Val Leu Ser Ser Leu Ile

420 425 430

Asn Glu Ser Val Ile Met Val Glu Ser Gly Val Cys Ser Arg Glu Asp

435 440 445

Ile Asp Val Ala Ala Val Ala Gly Val Asn Tyr Ala Gly Gly Ile Phe

450 455 460

Asp Trp Leu Gly Lys Leu Gly Glu Lys Asn Val Arg Thr Thr Leu Ser

465 470 475 480

Asn Leu Ala Gln Leu Leu His Ala Ala Arg Tyr Ala Pro His Tyr Thr

485 490 495

Leu Leu His Ala Ala Gln Pro Ala Leu Thr Thr Thr Pro

500 505

<210> 34

<211> 509

<212> PRT

<213> Serratia sp. OMLW3

<400> 34

Met Ala Glu Ser Asn Ala Ala Ile Gln Ser Ala Ala Ile Ile Gly Ala

1 5 10 15

Gly Thr Met Gly Arg Gly Ile Ala Tyr Leu Phe Ala Gln Lys Gly Ile

20 25 30

Arg Thr Val Leu Tyr Asn Arg Asn Gly Asn Thr Leu Asn Gln Ala Arg

35 40 45

Glu Tyr Ile Ala Gln Asp Leu Asn Lys Lys Val Glu Gln Gly Lys Ile

50 55 60

Ala Leu Gln Asp Lys Ser Ser Val Leu Ala Asn Leu Val Phe Ser Thr

65 70 75 80

Ala Leu Val Ala Ile Ala Asp Ser Glu Leu Val Ile Glu Thr Ile Ala

85 90 95

Glu His Glu Gln Thr Lys Leu Glu Val Leu Ala Ala Ile Ala Ala Val

100 105 110

Val Lys Pro Asp Thr Leu Ile Ala Thr Asn Thr Ser Ser Leu Ser Leu

115 120 125

Asn Lys Leu Ala Thr Ala Val Thr His Ser Glu Arg Phe Ile Gly Leu

130 135 140

His Phe Phe Asn Pro Ala Pro Leu Met Lys Leu Ile Glu Ile Ile Pro

145 150 155 160

Ala Tyr Phe Thr Ala His Ala Thr Thr Glu Arg Cys Arg Gln Leu Val

165 170 175

Ala Ala Leu Gly Lys Arg Asp Val Val Cys Gln Ala Thr Pro Gly Phe

180 185 190

Ile Val Asn Arg Met Ala Arg Pro Tyr Tyr Leu Glu Gly Phe Arg Leu

195 200 205

Leu Glu Glu His Val Ala Arg Ala Pro Gln Ile Asp Arg Ala Leu Lys

210 215 220

Ala Gly Gly His Phe Arg Met Gly Pro Leu Glu Leu Thr Asp Phe Ile

225 230 235 240

Gly Gln Asp Ile Asn Tyr Gln Val Ser Arg Gln Ile Trp Gln Asp Met

245 250 255

Gln Tyr Asp Pro Arg Tyr Thr Pro Gly His Leu Gln Arg Ser Leu Val

260 265 270

Asp Ala Gly Leu Leu Gly Lys Lys Asn Gly Arg Ser Tyr Phe Ala Ala

275 280 285

Glu Glu Thr Ala Pro Pro Val Thr Ala Ala Ile Asp Ala Asp Val Glu

290 295 300

Thr Leu Arg Val Tyr Gly Glu His Pro Phe Phe Thr Leu Leu Gln Gln

305 310 315 320

Arg Ala Ala Arg Gln Trp Pro Gln Leu Arg Val Glu Gln Arg Pro Ala

325 330 335

Leu Pro Ser Leu Gly Pro Ala Val Gln Ile Asn Asp Ala Phe Thr Val

340 345 350

Ser Ile Thr Asp Gly Arg Thr Ala Ser Gln Leu Ala Glu Gln Thr Thr

355 360 365

Ala Asp Ala Phe Val Val Asp Ile Ala Leu Asn Tyr Ala Asp Thr Ala

370 375 380

Tyr Leu Val Ala Ala His Asn Arg Tyr Ala Ser Ala Ala Asn Lys Ala

385 390 395 400

Leu Phe Leu Arg Leu Leu His Thr Ala Ile Pro Gln Val Glu Phe Ile

405 410 415

Lys Asp Ser Pro Ala Leu Ile Val Ala Arg Val Leu Ser Ser Leu Ile

420 425 430

Asn Glu Ser Val Ile Met Val Glu Ser Gly Val Cys Ser Arg Glu Asp

435 440 445

Ile Asp Val Ala Ala Val Ala Gly Val Asn Tyr Ala Gly Gly Ile Phe

450 455 460

Asp Trp Leu Gly Lys Leu Gly Glu Lys Asn Val Arg Thr Thr Leu Ser

465 470 475 480

Asn Leu Ala Gln Leu Leu His Ala Ala Arg Tyr Ala Pro His Tyr Thr

485 490 495

Leu Leu His Ala Ala Gln Pro Ala Leu Thr Thr Thr Pro

500 505

<210> 35

<211> 509

<212> PRT

<213> Serratia marcescens UMH11

<400> 35

Met Ala Glu Ser Asn Ala Ala Ile Gln Ser Ala Ala Ile Ile Gly Ala

1 5 10 15

Gly Thr Met Gly Arg Gly Ile Ala Tyr Leu Phe Ala Gln Lys Gly Ile

20 25 30

Arg Thr Val Leu Tyr Asn Arg Ser Gly Asn Thr Leu Asn Gln Ala Arg

35 40 45

Glu Tyr Ile Ala Gln Asp Leu Asn Lys Lys Val Glu Gln Gly Lys Ile

50 55 60

Ala Leu Gln Asp Lys Ser Ser Val Leu Ala Asn Leu Val Phe Ser Thr

65 70 75 80

Ala Leu Val Ala Ile Ala Asp Ser Glu Leu Val Ile Glu Thr Ile Ala

85 90 95

Glu His Glu Gln Thr Lys Leu Glu Val Leu Ala Ala Ile Ala Ala Val

100 105 110

Val Lys Pro Asp Thr Leu Ile Ala Thr Asn Thr Ser Ser Leu Ser Leu

115 120 125

Asn Lys Leu Ala Thr Ala Val Thr His Ser Glu Arg Phe Ile Gly Leu

130 135 140

His Phe Phe Asn Pro Ala Pro Leu Met Lys Leu Ile Glu Ile Ile Pro

145 150 155 160

Ala Tyr Phe Thr Ala His Ala Thr Thr Glu Arg Cys Arg Gln Leu Val

165 170 175

Ala Ala Leu Gly Lys Arg Asp Val Val Cys Gln Ala Thr Pro Gly Phe

180 185 190

Ile Val Asn Arg Met Ala Arg Pro Tyr Tyr Leu Glu Gly Phe Arg Leu

195 200 205

Leu Glu Glu His Val Ala Arg Ala Pro Gln Ile Asp Arg Ala Leu Lys

210 215 220

Ala Gly Gly His Phe Arg Met Gly Pro Leu Glu Leu Thr Asp Phe Ile

225 230 235 240

Gly Gln Asp Ile Asn Tyr Gln Val Ser Arg Gln Ile Trp Gln Asp Met

245 250 255

Gln Tyr Asp Pro Arg Tyr Thr Pro Gly His Leu Gln Arg Ser Leu Val

260 265 270

Asp Ala Gly Leu Leu Gly Lys Lys Asn Gly Arg Ser Tyr Phe Ala Ala

275 280 285

Glu Glu Thr Ala Pro Pro Val Thr Ala Ala Ile Asp Ala Asp Val Glu

290 295 300

Thr Leu Arg Val Tyr Gly Glu His Pro Phe Phe Thr Leu Leu Gln Gln

305 310 315 320

Arg Ala Ala Arg Gln Trp Pro Gln Leu Arg Val Glu Gln Arg Pro Ala

325 330 335

Leu Pro Ser Leu Gly Pro Ala Val Gln Ile Asn Asp Ala Phe Thr Val

340 345 350

Ser Ile Thr Asp Gly Arg Thr Ala Ser Gln Leu Ala Glu Gln Thr Thr

355 360 365

Ala Asp Ala Phe Val Val Asp Ile Ala Leu Asn Tyr Ala Asp Thr Ala

370 375 380

Tyr Leu Val Ala Ala His Asn Arg Tyr Ala Ser Ala Ala Asn Lys Ala

385 390 395 400

Leu Phe Leu Arg Leu Leu His Thr Ala Ile Pro Gln Val Glu Phe Ile

405 410 415

Lys Asp Ser Pro Ala Leu Ile Val Ala Arg Val Leu Ser Ser Leu Ile

420 425 430

Asn Glu Ser Val Ile Met Val Glu Ser Gly Val Cys Ser Arg Glu Asp

435 440 445

Ile Asp Val Ala Ala Val Ala Gly Val Asn Tyr Ala Gly Gly Ile Phe

450 455 460

Asp Trp Leu Gly Lys Leu Gly Glu Lys Asn Val Arg Thr Thr Leu Ser

465 470 475 480

Asn Leu Ala Gln Leu Leu His Ala Ala Arg Tyr Ala Pro His Tyr Thr

485 490 495

Leu Leu His Ala Ala Gln Pro Ala Leu Thr Thr Thr Pro

500 505

<210> 36

<211> 509

<212> PRT

<213> Serratia marcescens UMH1

<400> 36

Met Ala Glu Ser Asn Ala Ala Ile Gln Ser Ala Ala Ile Ile Gly Ala

1 5 10 15

Gly Thr Met Gly Arg Gly Ile Ala Tyr Leu Phe Ala Gln Lys Gly Ile

20 25 30

Arg Thr Val Leu Tyr Asn Arg Asn Gly Asn Thr Leu Asn Gln Ala Arg

35 40 45

Glu Tyr Ile Ala Gln Asp Leu Asn Lys Lys Val Glu Gln Gly Lys Ile

50 55 60

Ala Leu Gln Asp Lys Ser Ser Val Leu Ala Asn Leu Val Phe Ser Thr

65 70 75 80

Ala Leu Glu Ala Ile Ala Asp Ser Glu Leu Val Ile Glu Thr Ile Ala

85 90 95

Glu His Glu Gln Thr Lys Leu Glu Val Leu Ala Ala Ile Ala Ala Val

100 105 110

Val Lys Pro Asp Thr Leu Ile Ala Thr Asn Thr Ser Ser Leu Ser Leu

115 120 125

Asn Lys Leu Ala Thr Ala Val Thr His Ser Glu Arg Phe Ile Gly Leu

130 135 140

His Phe Phe Asn Pro Ala Pro Leu Met Lys Leu Ile Glu Ile Ile Pro

145 150 155 160

Ala Tyr Phe Thr Ala His Ala Thr Thr Glu Arg Cys Arg Gln Leu Val

165 170 175

Ala Ala Leu Gly Lys Arg Asp Val Val Cys Gln Ala Thr Pro Gly Phe

180 185 190

Ile Val Asn Arg Met Ala Arg Pro Tyr Tyr Leu Glu Gly Phe Arg Leu

195 200 205

Leu Glu Glu His Val Ala Arg Ala Pro Gln Ile Asp Arg Ala Leu Lys

210 215 220

Ala Gly Gly His Phe Arg Met Gly Pro Leu Glu Leu Thr Asp Phe Ile

225 230 235 240

Gly Gln Asp Ile Asn Tyr Gln Val Ser Arg Gln Ile Trp Gln Asp Met

245 250 255

Gln Tyr Asp Pro Arg Tyr Thr Pro Gly His Leu Gln Arg Ser Leu Val

260 265 270

Asp Ala Gly Leu Leu Gly Lys Lys Asn Gly Arg Ser Tyr Phe Ala Ala

275 280 285

Glu Glu Thr Ala Pro Pro Val Thr Ala Ala Ile Asp Ala Asp Val Glu

290 295 300

Thr Leu Arg Val Tyr Gly Glu His Pro Phe Phe Thr Leu Leu Gln Gln

305 310 315 320

Gln Ala Ala Arg Gln Trp Pro Gln Leu Arg Val Glu Gln Arg Pro Ala

325 330 335

Leu Pro Ser Leu Gly Pro Ala Val Gln Ile Asn Gly Ala Phe Thr Val

340 345 350

Ser Ile Thr Asp Gly Arg Thr Ala Ser Gln Leu Ala Glu Gln Thr Thr

355 360 365

Ala Asp Ala Phe Val Ile Asp Ile Ala Leu Asn Tyr Ala Asp Thr Ala

370 375 380

Tyr Leu Val Ala Ala His Asn Arg Tyr Ala Ser Ala Ala Asn Lys Ala

385 390 395 400

Leu Phe Leu Arg Leu Leu His Thr Ala Ile Pro Gln Val Glu Phe Ile

405 410 415

Lys Asp Ser Pro Ala Leu Ile Val Ala Arg Val Leu Ser Ser Leu Ile

420 425 430

Asn Glu Ser Val Ile Met Val Glu Ser Gly Val Cys Ser Arg Glu Asp

435 440 445

Ile Asp Val Ala Ala Val Ala Gly Val Asn Tyr Ala Gly Gly Ile Phe

450 455 460

Asp Trp Leu Gly Lys Leu Gly Glu Lys Asn Val Arg Thr Thr Leu Ser

465 470 475 480

Asn Leu Ala Gln Leu Leu His Ala Ala Arg Tyr Ala Pro His Tyr Thr

485 490 495

Leu Leu His Ala Ala Gln Pro Ala Leu Thr Thr Thr Pro

500 505

<210> 37

<211> 509

<212> PRT

<213> Serratia marcescens 2880STDY5683020

<400> 37

Met Ala Glu Ser Asn Ala Ala Ile Gln Ser Ala Ala Ile Ile Gly Ala

1 5 10 15

Gly Thr Met Gly Arg Gly Ile Ala Tyr Leu Phe Ala Gln Lys Gly Ile

20 25 30

Arg Thr Val Leu Tyr Asn Arg Asn Gly Asn Thr Leu Asn Gln Ala Arg

35 40 45

Glu Tyr Ile Ala Gln Asp Leu Asn Lys Lys Val Glu Gln Gly Lys Ile

50 55 60

Ala Leu Gln Asp Lys Gly Ala Val Leu Ala Asn Leu Val Phe Thr Ser

65 70 75 80

Val Phe Glu Thr Ile Ala Asp Ser Asp Leu Val Ile Glu Thr Ile Ala

85 90 95

Glu Gln Glu Gln Thr Lys Leu Glu Val Leu Ala Ala Ile Ala Ala Ala

100 105 110

Val Lys Pro Asp Thr Leu Ile Ala Thr Asn Thr Ser Ser Leu Ser Leu

115 120 125

Asn Lys Leu Ala Thr Ala Val Thr His Ser Glu Arg Phe Ile Gly Leu

130 135 140

His Phe Phe Asn Pro Ala Pro Leu Met Lys Leu Ile Glu Ile Ile Pro

145 150 155 160

Ala Tyr Phe Thr Ala Arg Ala Thr Thr Glu Arg Cys Arg Gln Leu Val

165 170 175

Ala Ala Leu Gly Lys Arg Asp Val Val Cys Gln Ala Thr Pro Gly Phe

180 185 190

Ile Val Asn Arg Met Ala Arg Pro Tyr Tyr Leu Glu Gly Phe Arg Leu

195 200 205

Leu Glu Glu His Val Ala Arg Ala Pro Gln Ile Asp Arg Ala Leu Lys

210 215 220

Ala Gly Gly His Phe Arg Met Gly Pro Leu Glu Leu Thr Asp Phe Ile

225 230 235 240

Gly Gln Asp Ile Asn Tyr Gln Val Ser Arg Gln Ile Trp Gln Asp Met

245 250 255

Gln Tyr Asp Pro Arg Tyr Thr Pro Gly His Leu Gln Arg Ser Leu Val

260 265 270

Asp Ala Gly Leu Leu Gly Lys Lys Asn Gly Arg Ser Tyr Phe Ala Ala

275 280 285

Glu Glu Asn Ala Leu Pro Val Thr Ala Ala Thr Asp Ala Asp Val Glu

290 295 300

Thr Leu Arg Val Tyr Gly Glu His Pro Phe Phe Thr Leu Leu Gln Gln

305 310 315 320

Arg Ala Ala Leu Gln Trp Pro Arg Leu Arg Val Glu Gln Arg Pro Ala

325 330 335

Leu Pro Gly Leu Gly Pro Ala Val Gln Ile Asn Glu Ala Phe Thr Val

340 345 350

Ser Val Thr Asp Gly Arg Thr Ala Ser Gln Leu Ala Glu Gln Thr Ala

355 360 365

Ala Asp Ala Phe Val Val Asp Val Ala Leu Asn Tyr Ala Asp Thr Ala

370 375 380

Tyr Leu Val Ala Ala His Asn Arg His Ala Ser Ala Ala Asn Lys Ala

385 390 395 400

Leu Phe Leu Arg Leu Leu His Thr Ala Ile Pro Gln Val Glu Phe Ile

405 410 415

Lys Asp Ser Pro Ala Leu Ile Val Ala Arg Val Leu Ser Ser Leu Ile

420 425 430

Asn Glu Ser Val Ile Met Val Glu Ser Gly Val Cys Ser Arg Glu Asp

435 440 445

Ile Asp Val Ala Ala Val Ala Gly Val Asn Tyr Ala Asp Gly Ile Phe

450 455 460

Gly Trp Leu Asp Ser Leu Gly Glu Lys Asn Val Arg Thr Thr Leu Ser

465 470 475 480

Asn Leu Ala Gln Leu Leu His Ala Ala Arg Tyr Ala Pro His Tyr Thr

485 490 495

Leu Leu His Ala Ala Gln Pro Ala Leu Thr Thr Thr Pro

500 505

<210> 38

<211> 509

<212> PRT

<213> Serratia marcescens 99

<400> 38

Met Ala Glu Ser Asn Ala Ala Ile Gln Ser Ala Ala Ile Ile Gly Ala

1 5 10 15

Gly Thr Met Gly Arg Gly Ile Ala Tyr Leu Phe Ala Gln Lys Gly Ile

20 25 30

Arg Thr Val Leu Tyr Asn Arg Asn Gly Asn Thr Leu Asn Gln Ala Arg

35 40 45

Glu Tyr Ile Ala Gln Asp Leu Asn Lys Lys Val Glu Gln Gly Lys Ile

50 55 60

Ala Leu Gln Asp Lys Gly Ala Val Leu Ala Asn Leu Val Phe Thr Ser

65 70 75 80

Val Phe Glu Thr Ile Ala Asp Ser Asp Leu Val Ile Glu Thr Ile Ala

85 90 95

Glu Gln Glu Gln Thr Lys Leu Glu Val Leu Ala Ala Ile Ala Ala Ala

100 105 110

Val Lys Pro Asp Thr Leu Ile Ala Thr Asn Thr Ser Ser Leu Ser Leu

115 120 125

Asn Lys Leu Ala Thr Ala Val Thr His Ser Glu Arg Phe Ile Gly Leu

130 135 140

His Phe Phe Asn Pro Ala Pro Leu Met Lys Leu Ile Glu Ile Ile Pro

145 150 155 160

Ala Tyr Phe Thr Ala Arg Ala Thr Thr Glu Arg Cys Arg Gln Leu Val

165 170 175

Ala Ala Leu Gly Lys Arg Asp Val Val Cys Gln Ala Thr Pro Gly Phe

180 185 190

Ile Val Asn Arg Met Ala Arg Pro Tyr Tyr Leu Glu Gly Phe Arg Leu

195 200 205

Leu Glu Glu His Val Ala Arg Ala Pro Gln Ile Asp Arg Ala Leu Lys

210 215 220

Ala Gly Gly His Phe Arg Met Gly Pro Leu Glu Leu Thr Asp Phe Ile

225 230 235 240

Gly Gln Asp Ile Asn Tyr Gln Val Ser Arg Gln Ile Trp Gln Asp Met

245 250 255

Gln Tyr Asp Pro Arg Tyr Thr Pro Gly His Leu Gln Arg Ser Leu Val

260 265 270

Asp Ala Gly Leu Leu Gly Lys Lys Asn Gly Arg Ser Tyr Phe Ala Ala

275 280 285

Glu Glu Asn Ala Leu Pro Val Thr Ala Ala Ser Asn Ala Asp Val Glu

290 295 300

Thr Leu Arg Val Tyr Gly Glu His Pro Phe Phe Thr Leu Leu Gln Gln

305 310 315 320

Arg Ala Ala Leu Gln Trp Pro Arg Leu Arg Val Glu Gln Arg Pro Ala

325 330 335

Leu Pro Gly Leu Gly Pro Ala Val Gln Ile Asn Glu Ala Phe Thr Val

340 345 350

Ser Val Thr Asp Gly Arg Thr Ala Ser Gln Leu Ala Glu Leu Thr Ala

355 360 365

Ala Asp Ala Phe Val Val Asp Val Ala Leu Asn Tyr Ala Asp Thr Ala

370 375 380

Tyr Leu Val Ala Ala His Asn Arg His Ala Ser Ala Ala Asn Lys Ala

385 390 395 400

Leu Phe Leu Arg Leu Leu His Thr Ala Ile Pro Gln Val Glu Phe Ile

405 410 415

Lys Asp Ser Pro Ala Leu Ile Val Ala Arg Val Leu Ser Ser Leu Ile

420 425 430

Asn Glu Ser Val Ile Met Val Glu Ser Gly Val Cys Ser Arg Glu Asp

435 440 445

Ile Asp Val Ala Ala Val Ala Gly Val Asn Tyr Ala Asp Gly Ile Phe

450 455 460

Gly Trp Leu Asp Ser Leu Gly Glu Lys Asn Val Arg Thr Thr Leu Ser

465 470 475 480

Asn Leu Ala Gln Leu Leu His Ala Ala Arg Tyr Ala Pro His Tyr Thr

485 490 495

Leu Leu His Ala Ala Gln Pro Val Leu Thr Thr Thr Pro

500 505

<210> 39

<211> 509

<212> PRT

<213> Serratia marcescens 374

<400> 39

Met Ala Glu Ser Asn Ala Ala Ile Gln Ser Ala Ala Ile Ile Gly Ala

1 5 10 15

Gly Thr Met Gly Arg Gly Ile Ala Tyr Leu Phe Ala Gln Lys Gly Ile

20 25 30

Arg Thr Val Leu Tyr Asn Arg Asn Gly Asn Thr Leu Asn Gln Ala Arg

35 40 45

Glu Tyr Ile Ala Gln Asp Leu Asn Lys Lys Val Glu Gln Gly Lys Ile

50 55 60

Ala Leu Gln Asp Lys Ser Ser Val Leu Ala Asn Leu Val Phe Ser Thr

65 70 75 80

Ala Leu Val Ala Ile Ala Asp Ser Glu Leu Val Ile Glu Thr Ile Ala

85 90 95

Glu His Glu Gln Thr Lys Leu Glu Val Leu Ala Ala Ile Ala Ala Val

100 105 110

Val Lys Pro Asp Thr Leu Ile Ala Thr Asn Thr Ser Ser Leu Ser Leu

115 120 125

Asn Lys Leu Ala Thr Ala Val Thr His Ser Glu Arg Phe Ile Gly Leu

130 135 140

His Phe Phe Asn Pro Ala Pro Leu Met Lys Leu Ile Glu Ile Ile Pro

145 150 155 160

Ala Tyr Phe Thr Ala His Ala Thr Thr Glu Arg Cys Arg Gln Leu Val

165 170 175

Ala Ala Leu Gly Lys Arg Asp Val Val Cys Gln Ala Thr Pro Gly Phe

180 185 190

Ile Val Asn Arg Met Ala Arg Pro Tyr Tyr Leu Glu Gly Phe Arg Leu

195 200 205

Leu Glu Glu His Val Ala Arg Ala Pro Gln Ile Asp Arg Ala Leu Lys

210 215 220

Ala Gly Gly His Phe Arg Met Gly Pro Leu Glu Leu Thr Asp Phe Ile

225 230 235 240

Gly Gln Asp Ile Asn Tyr Gln Val Ser Arg Gln Ile Trp Gln Asp Met

245 250 255

Gln Tyr Asp Pro Arg Tyr Thr Pro Gly His Leu Gln Arg Ser Leu Val

260 265 270

Asp Ala Gly Leu Leu Gly Lys Lys Asn Gly Arg Ser Tyr Phe Ala Ala

275 280 285

Glu Glu Thr Ala Pro Pro Val Thr Ala Ala Ile Asp Ala Asp Val Glu

290 295 300

Thr Leu Arg Val Tyr Gly Glu His Pro Phe Phe Thr Leu Leu Gln Gln

305 310 315 320

Gln Ala Ala Arg Gln Trp Pro Gln Leu Arg Val Glu Gln Arg Pro Ala

325 330 335

Leu Pro Ser Leu Gly Pro Ala Val Gln Ile Asn Gly Ala Phe Thr Val

340 345 350

Ser Ile Thr Asp Gly Arg Thr Ala Ser Gln Leu Ala Glu Gln Thr Thr

355 360 365

Ala Asp Ala Phe Val Ile Asp Ile Ala Leu Asn Tyr Ala Asp Thr Ala

370 375 380

Tyr Leu Val Ala Ala His Asn Arg Tyr Ala Ser Ala Ala Asn Lys Ala

385 390 395 400

Leu Phe Leu Arg Leu Leu His Thr Ala Ile Pro Gln Val Glu Phe Ile

405 410 415

Lys Asp Ser Pro Ala Leu Ile Val Ala Arg Val Leu Ser Ser Leu Ile

420 425 430

Asn Glu Ser Val Ile Met Val Glu Ser Gly Val Cys Ser Arg Glu Asp

435 440 445

Ile Asp Val Ala Ala Val Ala Gly Val Asn Tyr Ala Gly Gly Ile Phe

450 455 460

Asp Trp Leu Gly Lys Leu Gly Glu Lys Asn Val Arg Thr Thr Leu Ser

465 470 475 480

Asn Leu Ala Gln Leu Leu His Ala Ala Arg Tyr Ala Pro His Tyr Thr

485 490 495

Leu Leu His Ala Ala Gln Pro Ala Leu Thr Thr Thr Pro

500 505

<210> 40

<211> 509

<212> PRT

<213> Serratia marcescens 2880STDY5683036

<400> 40

Met Ala Glu Ser Asn Ala Ala Ile Gln Ser Ala Ala Ile Ile Gly Ala

1 5 10 15

Gly Thr Met Gly Arg Gly Ile Ala Tyr Leu Phe Ala Gln Lys Gly Ile

20 25 30

Arg Thr Val Leu Tyr Asn Arg Asn Gly Asn Thr Leu Asn Gln Ala Arg

35 40 45

Glu Tyr Ile Ala Gln Asp Leu Asn Lys Lys Val Glu Gln Gly Lys Ile

50 55 60

Ala Leu Gln Asp Lys Gly Ala Val Leu Ala Asn Leu Val Phe Thr Ser

65 70 75 80

Val Phe Glu Thr Ile Ala Asp Ser Asp Leu Val Ile Glu Thr Ile Ala

85 90 95

Glu Gln Glu Gln Thr Lys Leu Glu Val Leu Ala Ala Ile Ala Ala Ala

100 105 110

Val Lys Pro Asp Thr Leu Leu Ala Thr Asn Thr Ser Ser Leu Ser Leu

115 120 125

Asn Lys Leu Ala Thr Ala Val Thr His Ser Glu Arg Phe Ile Gly Leu

130 135 140

His Phe Phe Asn Pro Ala Pro Leu Met Lys Leu Ile Glu Ile Ile Pro

145 150 155 160

Ala Tyr Phe Thr Ala Arg Ala Thr Thr Glu Arg Cys Arg Gln Leu Val

165 170 175

Ala Ala Leu Gly Lys Arg Asp Val Val Cys Gln Ala Thr Pro Gly Phe

180 185 190

Ile Val Asn Arg Met Ala Arg Pro Tyr Tyr Leu Glu Gly Phe Arg Leu

195 200 205

Leu Glu Glu His Val Ala Arg Ala Pro Gln Ile Asp Arg Ala Leu Lys

210 215 220

Ala Gly Gly His Phe Arg Met Gly Pro Leu Glu Leu Thr Asp Phe Ile

225 230 235 240

Gly Gln Asp Ile Asn Tyr Gln Val Ser Arg Gln Ile Trp Gln Asp Met

245 250 255

Gln Tyr Asp Pro Arg Tyr Thr Pro Gly His Leu Gln Arg Ser Leu Val

260 265 270

Asp Ala Gly Leu Leu Gly Lys Lys Asn Gly Arg Ser Tyr Phe Ala Ala

275 280 285

Glu Glu Asn Ala Leu Pro Val Thr Ala Ala Ser Asn Ala Asp Val Glu

290 295 300

Thr Leu Arg Val Tyr Gly Glu His Pro Phe Phe Thr Leu Leu Gln Gln

305 310 315 320

Arg Ala Ala Leu Gln Trp Pro Arg Leu Arg Val Glu Gln Arg Pro Ala

325 330 335

Leu Pro Gly Leu Gly Pro Ala Val Gln Ile Asn Glu Ala Phe Thr Val

340 345 350

Ser Val Thr Asp Gly Arg Thr Ala Ser Gln Leu Ala Glu Leu Thr Ala

355 360 365

Ala Asp Ala Phe Val Val Asp Val Ala Leu Asn Tyr Ala Asp Thr Ala

370 375 380

Tyr Leu Val Ala Ala His Asn Arg His Ala Ser Ala Ala Asn Lys Ala

385 390 395 400

Leu Phe Leu Arg Leu Leu His Thr Ala Ile Pro Gln Val Glu Phe Ile

405 410 415

Lys Asp Ser Pro Ala Leu Ile Val Ala Arg Val Leu Ser Ser Leu Ile

420 425 430

Asn Glu Ser Val Ile Met Val Glu Ser Gly Val Cys Ser Arg Glu Asp

435 440 445

Ile Asp Val Ala Ala Val Ala Gly Val Asn Tyr Ala Asp Gly Ile Phe

450 455 460

Gly Trp Leu Asp Ser Leu Gly Glu Lys Asn Val Arg Thr Thr Leu Ser

465 470 475 480

Asn Leu Ala Gln Leu Leu His Ala Ala Arg Tyr Ala Pro His Tyr Thr

485 490 495

Leu Leu His Ala Ala Gln Pro Val Leu Thr Thr Thr Pro

500 505

<210> 41

<211> 509

<212> PRT

<213> Serratia marcescens 2880STDY5683034

<400> 41

Met Ala Glu Ser Asn Ala Ala Ile Gln Ser Ala Ala Ile Ile Gly Ala

1 5 10 15

Gly Thr Met Gly Arg Gly Ile Ala Tyr Leu Phe Ala Gln Lys Gly Ile

20 25 30

Arg Thr Val Leu Tyr Asn Arg Asn Gly Asn Thr Leu Asn Gln Ala Arg

35 40 45

Glu Tyr Ile Ala Gln Asp Leu Asn Lys Lys Val Glu Gln Gly Lys Ile

50 55 60

Ala Leu Gln Asp Lys Gly Ala Val Leu Ala Asn Leu Val Phe Thr Ser

65 70 75 80

Val Phe Glu Thr Ile Ala Asp Ser Glu Leu Val Ile Glu Thr Ile Ala

85 90 95

Glu Gln Glu Gln Thr Lys Leu Glu Val Leu Ala Ala Ile Ala Ala Ala

100 105 110

Val Lys Pro Asp Thr Leu Ile Ala Thr Asn Thr Ser Ser Leu Ser Leu

115 120 125

Asn Lys Leu Ala Thr Ala Val Thr His Ser Glu Arg Phe Ile Gly Leu

130 135 140

His Phe Phe Asn Pro Ala Pro Leu Met Lys Leu Ile Glu Ile Ile Pro

145 150 155 160

Ala Tyr Phe Thr Ala Arg Ala Thr Thr Glu Arg Cys Arg Gln Leu Val

165 170 175

Ala Ala Leu Gly Lys Arg Asp Val Val Cys Gln Ala Thr Pro Gly Phe

180 185 190

Ile Val Asn Arg Met Ala Arg Pro Tyr Tyr Leu Glu Gly Phe Arg Leu

195 200 205

Leu Glu Glu His Val Ala Arg Ala Pro Gln Ile Asp Arg Ala Leu Lys

210 215 220

Ala Gly Gly His Phe Arg Met Gly Pro Leu Glu Leu Thr Asp Phe Ile

225 230 235 240

Gly Gln Asp Ile Asn Tyr Gln Val Ser Arg Gln Ile Trp Gln Asp Met

245 250 255

Gln Tyr Asp Pro Arg Tyr Thr Pro Gly His Leu Gln Arg Ser Leu Val

260 265 270

Asp Ala Gly Leu Leu Gly Lys Lys Asn Gly Arg Ser Tyr Phe Ala Ala

275 280 285

Glu Glu Asn Ala Leu Pro Val Thr Ala Ala Thr Asp Ala Asp Val Glu

290 295 300

Thr Leu Arg Ile Tyr Gly Glu His Pro Phe Phe Thr Leu Leu Gln Gln

305 310 315 320

Arg Ala Ala Leu Gln Trp Pro Arg Leu Arg Val Glu Gln Arg Pro Ala

325 330 335

Leu Pro Gly Leu Gly Pro Ala Val Gln Ile Asn Glu Ala Phe Thr Val

340 345 350

Ser Val Thr Asp Gly Arg Thr Ala Ser Gln Leu Ala Glu Leu Thr Ala

355 360 365

Ala Asp Ala Phe Val Val Asp Val Ala Leu Asn Tyr Ala Asp Thr Ala

370 375 380

Tyr Leu Val Ala Ala His Asn Arg His Ala Ser Ala Ala Asn Lys Ala

385 390 395 400

Leu Phe Leu Arg Leu Leu His Thr Ala Ile Pro Gln Val Glu Phe Ile

405 410 415

Lys Asp Ser Pro Ala Leu Ile Val Ala Arg Val Leu Ser Ser Leu Ile

420 425 430

Asn Glu Ser Val Ile Met Val Glu Ser Gly Val Cys Ser Arg Glu Asp

435 440 445

Ile Asp Val Ala Ala Val Ala Gly Val Asn Tyr Ala Asp Gly Ile Phe

450 455 460

Gly Trp Leu Asp Asn Leu Gly Glu Lys Asn Val Arg Thr Thr Leu Ser

465 470 475 480

Asn Leu Ala Gln Leu Leu His Ala Ala Arg Tyr Ala Pro His Tyr Thr

485 490 495

Leu Leu His Ala Ala Gln Pro Ala Leu Thr Thr Thr Pro

500 505

<210> 42

<211> 509

<212> PRT

<213> Serratia marcescens 2880STDY5682892

<400> 42

Met Ala Glu Ser Asn Ala Ala Ile Gln Ser Ala Ala Ile Ile Gly Ala

1 5 10 15

Gly Thr Met Gly Arg Gly Ile Ala Tyr Leu Phe Ala Gln Lys Gly Ile

20 25 30

Arg Thr Val Leu Tyr Asn Arg Asn Gly Asn Thr Leu Asn Gln Ala Arg

35 40 45

Glu Tyr Ile Ala Gln Asp Leu Asn Lys Lys Val Glu Gln Gly Lys Ile

50 55 60

Ala Leu Gln Asp Lys Gly Ala Val Leu Ala Asn Leu Val Phe Thr Ser

65 70 75 80

Val Phe Glu Thr Ile Ala Asp Ser Asp Leu Val Ile Glu Thr Ile Ala

85 90 95

Glu Gln Glu Gln Thr Lys Leu Glu Val Leu Ala Ala Ile Ala Ala Ala

100 105 110

Val Lys Pro Asp Thr Leu Ile Ala Thr Asn Thr Ser Ser Leu Ser Leu

115 120 125

Asn Lys Leu Ala Thr Ala Val Thr His Ser Glu Arg Phe Ile Gly Leu

130 135 140

His Phe Phe Asn Pro Ala Pro Leu Met Lys Leu Ile Glu Ile Ile Pro

145 150 155 160

Ala Tyr Phe Thr Ala Arg Ala Thr Thr Glu Arg Cys Arg Gln Leu Val

165 170 175

Ala Ala Leu Gly Lys Arg Asp Val Val Cys Gln Ala Thr Pro Gly Phe

180 185 190

Ile Val Asn Arg Met Ala Arg Pro Tyr Tyr Leu Glu Gly Phe Arg Leu

195 200 205

Leu Glu Glu His Val Ala Arg Ala Pro Gln Ile Asp Arg Ala Leu Lys

210 215 220

Ala Gly Gly His Phe Arg Met Gly Pro Leu Glu Leu Thr Asp Phe Ile

225 230 235 240

Gly Gln Asp Ile Asn Tyr Gln Val Ser Arg Gln Ile Trp Gln Asp Met

245 250 255

Gln Tyr Asp Pro Arg Tyr Thr Pro Gly His Leu Gln Arg Ser Leu Val

260 265 270

Asp Ala Gly Leu Leu Gly Lys Lys Asn Gly Arg Ser Tyr Phe Ala Ala

275 280 285

Glu Glu Asn Ala Leu Pro Val Thr Ala Ala Thr Asp Ala Asp Val Glu

290 295 300

Thr Leu Arg Val Tyr Gly Glu His Pro Phe Phe Thr Leu Leu Gln Gln

305 310 315 320

Arg Ala Ala Leu Gln Trp Pro Arg Leu Arg Val Glu Gln Arg Pro Ala

325 330 335

Leu Pro Gly Leu Gly Pro Ala Val Gln Ile Asn Glu Ala Phe Thr Val

340 345 350

Ser Val Thr Asp Gly Arg Thr Ala Ser Gln Leu Ala Glu Leu Thr Ala

355 360 365

Ala Asp Ala Phe Val Val Asp Val Ala Leu Asn Tyr Ala Asp Thr Ala

370 375 380

Tyr Leu Val Ala Ala His Asn Arg His Ala Ser Ala Ala Asn Lys Ala

385 390 395 400

Leu Phe Leu Arg Leu Leu His Thr Ala Ile Pro Gln Val Glu Phe Ile

405 410 415

Lys Asp Ser Pro Ala Leu Ile Val Ala Arg Val Leu Ser Ser Leu Ile

420 425 430

Asn Glu Ser Val Ile Met Val Glu Ser Gly Val Cys Ser Arg Glu Asp

435 440 445

Ile Asp Val Ala Ala Val Ala Gly Val Asn Tyr Ala Asp Gly Ile Phe

450 455 460

Gly Trp Leu Asp Ser Leu Gly Glu Lys Asn Val Arg Thr Thr Leu Ser

465 470 475 480

Asn Leu Ala Gln Leu Leu His Ala Ala Arg Tyr Ala Pro His Tyr Thr

485 490 495

Leu Leu His Ala Ala Gln Pro Ala Leu Thr Thr Thr Pro

500 505

<210> 43

<211> 509

<212> PRT

<213> Serratia marcescens SM39

<400> 43

Met Ala Glu Ser Asn Ala Ala Ile Gln Ser Ala Ala Ile Ile Gly Ala

1 5 10 15

Gly Thr Met Gly Arg Gly Ile Ala Tyr Leu Phe Ala Gln Lys Gly Ile

20 25 30

Arg Thr Val Leu Tyr Asn Arg Asn Gly Asn Thr Leu Asn Gln Ala Arg

35 40 45

Glu Tyr Ile Val Gln Asp Leu Asn Lys Lys Val Glu Gln Gly Lys Ile

50 55 60

Ala Leu Gln Asp Lys Gly Ala Val Leu Ala Asn Leu Val Phe Thr Ser

65 70 75 80

Val Phe Glu Thr Ile Ala Asp Ser Glu Leu Val Ile Glu Thr Ile Ala

85 90 95

Glu Gln Glu Gln Thr Lys Leu Glu Val Leu Ala Ala Ile Ala Ala Ala

100 105 110

Val Lys Pro Asp Thr Leu Ile Ala Thr Asn Thr Ser Ser Leu Ser Leu

115 120 125

Asn Lys Leu Ala Thr Ala Val Thr His Ser Glu Arg Phe Ile Gly Leu

130 135 140

His Phe Phe Asn Pro Ala Pro Leu Met Lys Leu Ile Glu Ile Ile Pro

145 150 155 160

Ala Tyr Phe Thr Ala Arg Ala Thr Thr Glu Arg Cys Arg Gln Leu Val

165 170 175

Ala Ala Leu Gly Lys Arg Asp Val Val Cys Gln Ala Thr Pro Gly Phe

180 185 190

Ile Val Asn Arg Met Ala Arg Pro Tyr Tyr Leu Glu Gly Phe Arg Leu

195 200 205

Leu Glu Glu His Val Ala Arg Ala Pro Gln Ile Asp Arg Ala Leu Lys

210 215 220

Ala Gly Gly His Phe Arg Met Gly Pro Leu Glu Leu Thr Asp Phe Ile

225 230 235 240

Gly Gln Asp Ile Asn Tyr Gln Val Ser Arg Gln Ile Trp Gln Asp Met

245 250 255

Gln Tyr Asp Pro Arg Tyr Thr Pro Gly His Leu Gln Arg Ser Leu Val

260 265 270

Asp Ala Gly Leu Leu Gly Lys Lys Asn Gly Arg Ser Tyr Phe Ala Ala

275 280 285

Glu Glu Asn Ala Leu Pro Val Thr Ala Ala Thr Asp Ala Asp Val Glu

290 295 300

Thr Leu Arg Ile Tyr Gly Glu His Pro Phe Phe Thr Leu Leu Gln Gln

305 310 315 320

Arg Ala Ala Leu Gln Trp Pro Arg Leu Arg Val Glu Gln Arg Pro Ala

325 330 335

Leu Pro Gly Leu Gly Pro Ala Val Gln Ile Asn Glu Ala Phe Thr Val

340 345 350

Ser Val Thr Asp Gly Arg Thr Ala Ser Gln Leu Ala Glu Leu Thr Ala

355 360 365

Ala Asp Ala Phe Val Val Asp Val Ala Leu Asn Tyr Ala Asp Thr Ala

370 375 380

Tyr Leu Val Ala Ala His Asn Arg His Ala Ser Ala Ala Asn Lys Ala

385 390 395 400

Leu Phe Leu Arg Leu Leu His Thr Ala Ile Pro Gln Val Glu Phe Ile

405 410 415

Lys Asp Ser Pro Ala Leu Ile Val Ala Arg Val Leu Ser Ser Leu Ile

420 425 430

Asn Glu Ser Val Ile Met Val Glu Ser Gly Val Cys Ser Arg Glu Asp

435 440 445

Ile Asp Val Ala Ala Val Ala Gly Val Asn Tyr Ala Asp Gly Ile Phe

450 455 460

Gly Trp Leu Asp Asn Leu Gly Glu Lys Asn Val Arg Thr Thr Leu Ser

465 470 475 480

Asn Leu Ala Gln Leu Leu His Ala Ala Arg Tyr Ala Pro His Tyr Thr

485 490 495

Leu Leu His Ala Ala Gln Pro Ala Leu Thr Thr Thr Pro

500 505

<210> 44

<211> 509

<212> PRT

<213> Serratia marcescens 189

<400> 44

Met Ala Glu Ser Asn Ala Ala Ile Gln Ser Ala Ala Ile Ile Gly Ala

1 5 10 15

Gly Thr Met Gly Arg Gly Ile Ala Tyr Leu Phe Ala Gln Lys Gly Ile

20 25 30

Arg Thr Val Leu Tyr Asn Arg Asn Gly Asn Thr Leu Asn Gln Ala Arg

35 40 45

Glu Tyr Ile Ala Gln Asp Leu Asn Lys Lys Val Glu Gln Gly Lys Ile

50 55 60

Ala Leu Gln Asp Lys Gly Ala Val Leu Ala Asn Leu Val Phe Thr Ser

65 70 75 80

Val Phe Glu Thr Ile Ala Asp Ser Asp Leu Val Ile Glu Thr Ile Ala

85 90 95

Glu Gln Glu Gln Thr Lys Leu Glu Val Leu Ala Ala Ile Ala Ala Ala

100 105 110

Val Lys Pro Asp Thr Leu Ile Ala Thr Asn Thr Ser Ser Leu Ser Leu

115 120 125

Asn Lys Leu Ala Thr Ala Val Thr His Ser Glu Arg Phe Ile Gly Leu

130 135 140

His Phe Phe Asn Pro Ala Pro Leu Met Lys Leu Ile Glu Ile Ile Pro

145 150 155 160

Ala Tyr Phe Thr Ala Arg Ala Thr Thr Glu Arg Cys Arg Gln Leu Val

165 170 175

Ala Ala Leu Gly Lys Arg Asp Val Val Cys Gln Ala Thr Pro Gly Phe

180 185 190

Ile Val Asn Arg Met Ala Arg Pro Tyr Tyr Leu Glu Gly Phe Arg Leu

195 200 205

Leu Glu Glu His Val Ala Arg Ala Pro Gln Ile Asp Arg Ala Leu Lys

210 215 220

Ala Gly Gly His Phe Arg Met Gly Pro Leu Glu Leu Thr Asp Phe Ile

225 230 235 240

Gly Gln Asp Ile Asn Tyr Gln Val Ser Arg Gln Ile Trp Gln Asp Met

245 250 255

Gln Tyr Asp Pro Arg Tyr Thr Pro Gly His Leu Gln Arg Ser Leu Val

260 265 270

Asp Ala Gly Leu Leu Gly Lys Lys Asn Gly Arg Ser Tyr Phe Ala Ala

275 280 285

Glu Glu Asn Ala Leu Pro Val Thr Ala Ala Thr Asp Ala Asp Val Glu

290 295 300

Thr Leu Arg Ile Tyr Gly Glu His Pro Phe Phe Thr Leu Leu Gln Gln

305 310 315 320

Arg Ala Ala Leu Gln Trp Pro Arg Leu Arg Val Glu Gln Arg Pro Ala

325 330 335

Leu Pro Gly Leu Gly Pro Ala Val Gln Ile Asn Glu Ala Phe Thr Val

340 345 350

Ser Val Thr Asp Gly Arg Thr Ala Ser Gln Leu Ala Glu Leu Thr Ala

355 360 365

Ala Asp Ala Phe Val Val Asp Val Ala Leu Asn Tyr Ala Asp Thr Ala

370 375 380

Tyr Leu Val Ala Ala His Asn Arg His Ala Ser Ala Ala Asn Lys Ala

385 390 395 400

Leu Phe Leu Arg Leu Leu His Thr Ala Ile Pro Gln Val Glu Phe Ile

405 410 415

Lys Asp Ser Pro Ala Leu Ile Val Ala Arg Val Leu Ser Ser Leu Ile

420 425 430

Asn Glu Ser Val Ile Met Val Glu Ser Gly Val Cys Ser Arg Glu Asp

435 440 445

Ile Asp Val Ala Ala Val Ala Gly Val Asn Tyr Ala Asp Gly Ile Phe

450 455 460

Gly Trp Leu Asp Asn Leu Gly Glu Lys Asn Val Arg Thr Thr Leu Ser

465 470 475 480

Asn Leu Ala Gln Leu Leu His Ala Ala Arg Tyr Ala Pro His Tyr Thr

485 490 495

Leu Leu His Ala Ala Gln Pro Ala Leu Thr Thr Thr Pro

500 505

<210> 45

<211> 509

<212> PRT

<213> Serratia marcescens SMB2099

<400> 45

Met Ala Glu Ser Asn Ala Ala Ile Gln Ser Ala Ala Ile Ile Gly Ala

1 5 10 15

Gly Thr Met Gly Arg Gly Ile Ala Tyr Leu Phe Ala Gln Lys Gly Ile

20 25 30

Arg Thr Val Leu Tyr Asn Arg Asn Gly Asn Thr Leu Asn Gln Ala Arg

35 40 45

Glu Tyr Ile Ala Gln Asp Leu Asn Lys Lys Val Glu Gln Gly Lys Ile

50 55 60

Ala Leu Gln Asp Lys Gly Ala Val Leu Ala Asn Leu Val Phe Thr Ser

65 70 75 80

Val Phe Glu Thr Ile Ala Asp Ser Asp Leu Val Ile Glu Thr Ile Ala

85 90 95

Glu Gln Glu Gln Thr Lys Leu Glu Val Leu Ala Ala Ile Ala Ala Ala

100 105 110

Val Lys Pro Asp Thr Leu Ile Ala Thr Asn Thr Ser Ser Leu Ser Leu

115 120 125

Asn Lys Leu Ala Thr Ala Val Thr His Ser Glu Arg Phe Ile Gly Leu

130 135 140

His Phe Phe Asn Pro Ala Pro Leu Met Lys Leu Ile Glu Ile Ile Pro

145 150 155 160

Ala Tyr Phe Thr Ala Arg Ala Thr Thr Glu Arg Cys Arg Gln Leu Val

165 170 175

Ala Ala Leu Gly Lys Arg Asp Val Val Cys Gln Ala Thr Pro Gly Phe

180 185 190

Ile Val Asn Arg Met Ala Arg Pro Tyr Tyr Leu Glu Gly Phe Arg Leu

195 200 205

Leu Glu Glu His Val Ala Arg Ala Pro Gln Ile Asp Arg Ala Leu Lys

210 215 220

Ala Gly Gly His Phe Arg Met Gly Pro Leu Glu Leu Thr Asp Phe Ile

225 230 235 240

Gly Gln Asp Ile Asn Tyr Gln Val Ser Arg Gln Ile Trp Gln Asp Met

245 250 255

Gln Tyr Asp Pro Arg Tyr Thr Pro Gly His Leu Gln Arg Ser Leu Val

260 265 270

Asp Ala Gly Leu Leu Gly Lys Lys Asn Gly Arg Ser Tyr Phe Ala Ala

275 280 285

Glu Glu Asn Ala Leu Pro Val Thr Ala Ala Thr Asp Ala Asp Val Glu

290 295 300

Thr Leu Arg Val Tyr Gly Glu His Pro Phe Phe Thr Leu Leu Gln Gln

305 310 315 320

Arg Ala Ala Leu Gln Trp Pro Arg Leu Arg Val Glu Gln Arg Pro Ala

325 330 335

Leu Pro Gly Leu Gly Pro Ala Val Gln Ile Asn Glu Ala Phe Thr Val

340 345 350

Ser Val Thr Asp Gly Arg Thr Ala Ser Gln Leu Ala Glu Leu Thr Ala

355 360 365

Ala Asp Ala Phe Val Val Asp Val Ala Leu Asn Tyr Ala Asp Thr Ala

370 375 380

Tyr Leu Val Ala Ala His Asn Arg His Ala Ser Glu Ala Asn Lys Ala

385 390 395 400

Leu Phe Leu Arg Leu Leu His Thr Ala Ile Pro Gln Val Glu Phe Ile

405 410 415

Lys Asp Ser Pro Ala Leu Ile Val Ala Arg Val Leu Ser Ser Leu Ile

420 425 430

Asn Glu Ser Val Ile Met Val Glu Ser Gly Val Cys Ser Arg Glu Asp

435 440 445

Ile Asp Val Ala Ala Val Ala Gly Val Asn Tyr Ala Asp Gly Ile Phe

450 455 460

Gly Trp Leu Asp Ser Leu Gly Glu Lys Asn Val Arg Thr Thr Leu Ser

465 470 475 480

Asn Leu Ala Gln Leu Leu His Ala Ala Arg Tyr Ala Pro His Tyr Thr

485 490 495

Leu Leu His Ala Ala Gln Pro Ala Leu Thr Thr Thr Pro

500 505

<210> 46

<211> 509

<212> PRT

<213> Serratia marcescens 2880STDY5682862

<400> 46

Met Ala Glu Ser Asn Ala Ala Ile Gln Ser Ala Ala Ile Ile Gly Ala

1 5 10 15

Gly Thr Met Gly Arg Gly Ile Ala Tyr Leu Phe Ala Gln Lys Gly Ile

20 25 30

Arg Thr Val Leu Tyr Asn Arg Asn Gly Asn Thr Leu Asn Gln Ala Arg

35 40 45

Glu Tyr Ile Val Gln Asp Leu Asn Lys Lys Val Glu Gln Gly Lys Ile

50 55 60

Ala Leu Gln Asp Lys Gly Ala Val Leu Ala Asn Leu Val Phe Thr Ser

65 70 75 80

Val Phe Glu Thr Ile Ala Asp Ser Glu Leu Val Ile Glu Thr Ile Ala

85 90 95

Glu Gln Glu Gln Thr Lys Leu Glu Val Leu Ala Ala Ile Ala Ala Ala

100 105 110

Val Lys Pro Asp Thr Leu Ile Ala Thr Asn Thr Ser Ser Leu Ser Leu

115 120 125

Asn Lys Leu Ala Thr Ala Val Thr His Ser Glu Arg Phe Ile Gly Leu

130 135 140

His Phe Phe Asn Pro Ala Pro Leu Met Lys Leu Ile Glu Ile Ile Pro

145 150 155 160

Ala Tyr Phe Thr Ala Arg Ala Thr Thr Glu Arg Cys Arg Gln Leu Val

165 170 175

Ala Ala Leu Gly Lys Arg Asp Val Val Cys Gln Ala Thr Pro Gly Phe

180 185 190

Ile Val Asn Arg Met Ala Arg Pro Tyr Tyr Leu Glu Gly Phe Arg Leu

195 200 205

Leu Glu Glu His Val Ala Arg Ala Pro Gln Ile Asp Arg Ala Leu Lys

210 215 220

Ala Gly Gly His Phe Arg Met Gly Pro Leu Glu Leu Thr Asp Phe Ile

225 230 235 240

Gly Gln Asp Ile Asn Tyr Gln Val Ser Arg Gln Ile Trp Gln Asp Met

245 250 255

Gln Tyr Asp Pro Arg Tyr Thr Pro Gly His Leu Gln Arg Ser Leu Val

260 265 270

Asp Ala Gly Leu Leu Gly Lys Lys Asn Gly Arg Ser Tyr Phe Ala Ala

275 280 285

Glu Glu Asn Ala Leu Pro Val Thr Ala Ala Thr Asp Ala Asp Val Glu

290 295 300

Thr Leu Arg Val Tyr Gly Glu His Pro Phe Phe Thr Leu Leu Gln Gln

305 310 315 320

Arg Ala Ala Leu Gln Trp Pro Arg Leu Arg Val Glu Gln Arg Pro Ala

325 330 335

Leu Pro Gly Leu Gly Pro Ala Val Gln Ile Asn Glu Ala Phe Thr Val

340 345 350

Ser Val Thr Asp Gly Arg Thr Ala Ser Gln Leu Ala Glu Leu Thr Ala

355 360 365

Ala Asp Ala Phe Val Val Asp Val Ala Leu Asn Tyr Ala Asp Thr Ala

370 375 380

Tyr Leu Val Ala Ala His Asn Arg His Ala Ser Ala Ala Asn Lys Ala

385 390 395 400

Leu Phe Leu Arg Leu Leu His Thr Ala Ile Pro Gln Val Glu Phe Ile

405 410 415

Lys Asp Ser Pro Ala Leu Ile Val Ala Arg Val Leu Ser Ser Leu Ile

420 425 430

Asn Glu Ser Val Ile Met Val Glu Ser Gly Val Cys Ser Arg Glu Asp

435 440 445

Ile Asp Val Ala Ala Val Ala Gly Val Asn Tyr Ala Asp Gly Ile Phe

450 455 460

Gly Trp Leu Asp Ser Leu Gly Glu Lys Asn Val Arg Thr Thr Leu Ser

465 470 475 480

Asn Met Ala Gln Leu Leu His Ala Ala Arg Tyr Ala Pro His Tyr Thr

485 490 495

Leu Leu His Ala Ala Gln Pro Ala Leu Met Thr Thr Pro

500 505

<210> 47

<211> 509

<212> PRT

<213> Serratia marcescens SE4145

<400> 47

Met Ala Glu Ser Asn Ala Glu Ile Gln Ser Ala Ala Ile Ile Gly Ala

1 5 10 15

Gly Thr Met Gly Arg Gly Ile Ala Tyr Leu Phe Ala Gln Lys Gly Ile

20 25 30

Arg Thr Val Leu Tyr Asn Arg Asn Gly Asn Thr Leu Asn Gln Ala Arg

35 40 45

Glu Tyr Ile Val Gln Asp Leu Asn Lys Lys Val Glu Gln Gly Lys Ile

50 55 60

Ala Leu Gln Asp Lys Gly Ala Val Leu Ala Asn Leu Val Phe Thr Ser

65 70 75 80

Val Phe Glu Thr Ile Ala Asp Ser Glu Leu Val Ile Glu Thr Ile Ala

85 90 95

Glu Gln Glu Gln Thr Lys Leu Glu Val Leu Ala Ala Ile Ala Ala Ala

100 105 110

Val Lys Pro Asp Thr Leu Ile Ala Thr Asn Thr Ser Ser Leu Ser Leu

115 120 125

Asn Lys Leu Ala Thr Ala Val Thr His Ser Glu Arg Phe Ile Gly Leu

130 135 140

His Phe Phe Asn Pro Ala Pro Leu Met Lys Leu Ile Glu Ile Ile Pro

145 150 155 160

Ala Tyr Phe Thr Ala Arg Ala Thr Thr Glu Arg Cys Arg Gln Leu Val

165 170 175

Ala Ala Leu Gly Lys Arg Asp Val Val Cys Gln Ala Thr Pro Gly Phe

180 185 190

Ile Val Asn Arg Met Ala Arg Pro Tyr Tyr Leu Glu Gly Phe Arg Leu

195 200 205

Leu Glu Glu His Val Ala Arg Ala Pro Gln Ile Asp Arg Ala Leu Lys

210 215 220

Ala Gly Gly His Phe Arg Met Gly Pro Leu Glu Leu Thr Asp Phe Ile

225 230 235 240

Gly Gln Asp Ile Asn Tyr Gln Val Ser Arg Gln Ile Trp Gln Asp Met

245 250 255

Gln Tyr Asp Pro Arg Tyr Thr Pro Gly His Leu Gln Arg Ser Leu Val

260 265 270

Asp Ala Gly Leu Leu Gly Lys Lys Asn Gly Arg Ser Tyr Phe Ala Ala

275 280 285

Glu Glu Asn Ala Leu Pro Val Thr Ala Ala Thr Asp Ala Asp Val Glu

290 295 300

Thr Leu Arg Ile Tyr Gly Glu His Pro Phe Phe Thr Leu Leu Gln Gln

305 310 315 320

Arg Ala Ala Leu Gln Trp Pro Arg Leu Arg Val Glu Gln Arg Pro Ala

325 330 335

Leu Pro Gly Leu Gly Pro Ala Val Gln Ile Asn Glu Ala Phe Thr Val

340 345 350

Ser Val Thr Asp Gly Arg Thr Ala Ser Gln Leu Ala Glu Leu Thr Ala

355 360 365

Ala Asp Ala Phe Val Val Asp Val Ala Leu Asn Tyr Ala Asp Thr Ala

370 375 380

Tyr Leu Val Ala Ala His Asn Arg His Ala Ser Ala Ala Asn Lys Ala

385 390 395 400

Leu Phe Leu Arg Leu Leu His Thr Ala Ile Pro Gln Val Glu Phe Ile

405 410 415

Lys Asp Ser Pro Ala Leu Ile Val Ala Arg Val Leu Ser Ser Leu Ile

420 425 430

Asn Glu Ser Val Ile Met Val Glu Ser Gly Val Cys Ser Arg Glu Asp

435 440 445

Ile Asp Val Ala Ala Val Ala Gly Val Asn Tyr Ala Asp Gly Ile Phe

450 455 460

Gly Trp Leu Asp Asn Leu Gly Glu Lys Asn Val Arg Thr Thr Leu Ser

465 470 475 480

Asn Leu Ala Gln Leu Leu His Ala Ala Arg Tyr Ala Pro His Tyr Thr

485 490 495

Leu Leu His Ala Ala Gln Pro Ala Leu Thr Thr Thr Pro

500 505

<210> 48

<211> 509

<212> PRT

<213> Serratia marcescens 2880STDY5682876

<400> 48

Met Ala Glu Ser Asn Ala Ala Ile Gln Ser Ala Ala Ile Ile Gly Ala

1 5 10 15

Gly Thr Met Gly Arg Gly Ile Ala Tyr Leu Phe Ala Gln Lys Gly Ile

20 25 30

Arg Thr Val Leu Tyr Asn Arg Asn Gly Asn Thr Leu Asn Gln Ala Arg

35 40 45

Glu Tyr Ile Ala Gln Asp Leu Asn Lys Lys Val Glu Gln Gly Lys Ile

50 55 60

Ala Leu Gln Gly Lys Gly Ala Val Leu Ala Asn Leu Val Phe Thr Ser

65 70 75 80

Val Phe Glu Thr Ile Ala Asp Ser Asp Leu Val Ile Glu Thr Ile Ala

85 90 95

Glu Gln Glu Gln Thr Lys Leu Glu Val Leu Ala Ala Ile Ala Ala Ala

100 105 110

Val Lys Pro Asp Thr Leu Ile Ala Thr Asn Thr Ser Ser Leu Ser Leu

115 120 125

Asn Lys Leu Ala Thr Ala Val Thr His Ser Glu Arg Phe Ile Gly Leu

130 135 140

His Phe Phe Asn Pro Ala Pro Leu Met Lys Leu Ile Glu Ile Ile Pro

145 150 155 160

Ala Tyr Phe Thr Ala Arg Ala Thr Thr Glu Arg Cys Arg Gln Leu Val

165 170 175

Ala Ala Leu Gly Lys Arg Asp Val Val Cys Gln Ala Thr Pro Gly Phe

180 185 190

Ile Val Asn Arg Met Ala Arg Pro Tyr Tyr Leu Glu Gly Phe Arg Leu

195 200 205

Leu Glu Glu His Val Ala Arg Ala Pro Gln Ile Asp Arg Ala Leu Lys

210 215 220

Ala Gly Gly His Phe Arg Met Gly Pro Leu Glu Leu Thr Asp Phe Ile

225 230 235 240

Gly Gln Asp Ile Asn Tyr Gln Val Ser Arg Gln Ile Trp Gln Asp Met

245 250 255

Gln Tyr Asp Pro Arg Tyr Thr Pro Gly His Leu Gln Arg Ser Leu Val

260 265 270

Asp Ala Gly Leu Leu Gly Lys Lys Asn Gly Arg Ser Tyr Phe Ala Ala

275 280 285

Glu Glu Asn Ala Leu Pro Val Thr Ala Ala Thr Asp Ala Asp Val Glu

290 295 300

Thr Leu Arg Val Tyr Gly Glu His Pro Phe Phe Thr Leu Leu Gln Gln

305 310 315 320

Arg Ala Ala Leu Gln Trp Pro Arg Leu Arg Val Glu Gln Arg Pro Ala

325 330 335

Leu Pro Gly Leu Gly Pro Ala Val Gln Ile Asn Glu Ala Phe Thr Val

340 345 350

Ser Val Thr Asp Gly Arg Thr Ala Ser Gln Leu Ala Glu Leu Thr Ala

355 360 365

Ala Asp Ala Phe Val Val Asp Val Ala Leu Asn Tyr Ala Asp Thr Ala

370 375 380

Tyr Leu Val Ala Ala His Asn Arg His Ala Ser Ala Ala Asn Lys Ala

385 390 395 400

Leu Phe Leu Arg Leu Leu His Thr Ala Ile Pro Gln Val Glu Phe Ile

405 410 415

Lys Asp Ser Pro Ala Leu Ile Val Ala Arg Val Leu Ser Ser Leu Ile

420 425 430

Asn Glu Ser Val Ile Met Val Glu Ser Gly Val Cys Ser Arg Glu Asp

435 440 445

Ile Asp Val Ala Ala Val Ala Gly Val Asn Tyr Ala Asp Gly Ile Phe

450 455 460

Gly Trp Leu Asp Ser Leu Gly Glu Lys Asn Val Arg Thr Thr Leu Ser

465 470 475 480

Asn Leu Ala Gln Leu Leu His Ala Ala Arg Tyr Ala Pro His Tyr Thr

485 490 495

Leu Leu His Ala Ala Gln Pro Ala Leu Thr Thr Thr Pro

500 505

<210> 49

<211> 509

<212> PRT

<213> Serratia marcescens 709

<400> 49

Met Ala Glu Ser Asn Ala Ala Ile Gln Ser Ala Ala Ile Ile Gly Ala

1 5 10 15

Gly Thr Met Gly Arg Gly Ile Ala Tyr Leu Phe Ala Gln Lys Gly Ile

20 25 30

Arg Thr Val Leu Tyr Asn Arg Asn Gly Asn Thr Leu Asn Gln Ala Arg

35 40 45

Glu Tyr Ile Ala Gln Asp Leu Asn Lys Lys Val Glu Gln Gly Lys Ile

50 55 60

Ala Leu Gln Asp Lys Gly Ala Val Leu Ala Asn Leu Val Phe Thr Ser

65 70 75 80

Val Phe Glu Thr Ile Ala Asp Ser Asp Leu Val Ile Glu Thr Ile Ala

85 90 95

Glu Gln Glu Gln Thr Lys Leu Glu Val Leu Ala Ala Ile Ala Ala Ala

100 105 110

Val Lys Pro Asp Thr Leu Ile Ala Thr Asn Thr Ser Ser Leu Ser Leu

115 120 125

Asn Lys Leu Ala Thr Ala Val Thr His Ser Glu Arg Phe Ile Gly Leu

130 135 140

His Phe Phe Asn Pro Ala Pro Leu Met Lys Leu Ile Glu Ile Ile Pro

145 150 155 160

Ala Tyr Phe Thr Ala Arg Ala Thr Thr Glu Arg Cys Arg Gln Leu Val

165 170 175

Ala Ala Leu Gly Lys Arg Asp Val Val Cys Gln Ala Thr Pro Gly Phe

180 185 190

Ile Val Asn Arg Met Ala Arg Pro Tyr Tyr Leu Glu Gly Phe Arg Leu

195 200 205

Leu Glu Glu His Val Ala Arg Ala Pro Gln Ile Asp Arg Ala Leu Lys

210 215 220

Ala Gly Gly His Phe Arg Met Gly Pro Leu Glu Leu Thr Asp Phe Ile

225 230 235 240

Gly Gln Asp Ile Asn Tyr Gln Val Ser Arg Gln Ile Trp Gln Asp Met

245 250 255

Gln Tyr Asp Pro Arg Tyr Thr Pro Gly His Leu Gln Arg Ser Leu Val

260 265 270

Asp Ala Gly Leu Leu Gly Lys Lys Asn Gly Arg Ser Tyr Phe Ala Ala

275 280 285

Glu Glu Asn Ala Leu Pro Val Thr Ala Ala Thr Asp Ala Asp Val Glu

290 295 300

Thr Leu Arg Val Tyr Asp Glu His Pro Phe Phe Thr Leu Leu Gln Gln

305 310 315 320

Arg Ala Ala Leu Gln Trp Pro Arg Leu Arg Val Glu Gln Arg Pro Ala

325 330 335

Leu Pro Gly Leu Gly Pro Ala Val Gln Ile Asn Glu Ala Phe Thr Val

340 345 350

Ser Val Thr Asp Gly Arg Thr Ala Ser Gln Leu Ala Glu Leu Thr Ala

355 360 365

Ala Asp Ala Phe Val Val Asp Val Ala Leu Asn Tyr Ala Asp Thr Ala

370 375 380

Tyr Leu Val Ala Ala His Asn Arg His Ala Ser Ala Ala Asn Lys Ala

385 390 395 400

Leu Phe Leu Arg Leu Leu His Thr Ala Ile Pro Gln Val Glu Phe Ile

405 410 415

Lys Asp Ser Pro Ala Leu Ile Val Ala Arg Val Leu Ser Ser Leu Ile

420 425 430

Asn Glu Ser Val Ile Met Val Glu Ser Gly Val Cys Ser Arg Glu Asp

435 440 445

Ile Asp Val Ala Ala Val Ala Gly Val Asn Tyr Ala Asp Gly Ile Phe

450 455 460

Gly Trp Leu Asp Ser Leu Gly Glu Lys Asn Val Arg Thr Thr Leu Ser

465 470 475 480

Asn Leu Ala Gln Leu Leu His Ala Ala Arg Tyr Ala Pro His Tyr Thr

485 490 495

Leu Leu His Ala Ala Gln Pro Ala Leu Thr Thr Thr Pro

500 505

<210> 50

<211> 509

<212> PRT

<213> Serratia marcescens MGH136

<400> 50

Met Ala Glu Ser Asn Ala Ala Ile Gln Ser Ala Ala Ile Ile Gly Ala

1 5 10 15

Gly Thr Met Gly Arg Gly Ile Ala Tyr Leu Phe Ala Gln Lys Gly Ile

20 25 30

Arg Thr Val Leu Tyr Asn Arg Asn Gly Asn Thr Leu Asn Gln Ala Arg

35 40 45

Glu Tyr Ile Ala Gln Asp Leu Asn Lys Lys Val Glu Gln Asp Lys Ile

50 55 60

Ala Leu Gln Asp Lys Gly Ala Val Leu Ala Asn Leu Val Phe Thr Ser

65 70 75 80

Val Phe Glu Thr Ile Ala Asp Ser Asp Leu Val Ile Glu Thr Ile Ala

85 90 95

Glu Gln Glu Gln Thr Lys Leu Glu Val Leu Ala Ala Ile Ala Ala Ala

100 105 110

Val Lys Pro Asp Thr Leu Ile Ala Thr Asn Thr Ser Ser Leu Ser Leu

115 120 125

Asn Lys Leu Ala Thr Ala Val Thr His Ser Glu Arg Phe Ile Gly Leu

130 135 140

His Phe Phe Asn Pro Ala Pro Leu Met Lys Leu Ile Glu Ile Ile Pro

145 150 155 160

Ala Tyr Phe Thr Ala Arg Val Thr Thr Glu Arg Cys Arg Gln Leu Val

165 170 175

Ala Ala Leu Gly Lys Arg Asp Val Val Cys Gln Ala Thr Pro Gly Phe

180 185 190

Ile Val Asn Arg Met Ala Arg Pro Tyr Tyr Leu Glu Gly Phe Arg Leu

195 200 205

Leu Glu Glu His Val Ala Arg Ala Pro Gln Ile Asp Arg Ala Leu Lys

210 215 220

Ala Gly Gly His Phe Arg Met Gly Pro Leu Glu Leu Thr Asp Phe Ile

225 230 235 240

Gly Gln Asp Ile Asn Tyr Gln Val Ser Arg Gln Ile Trp Gln Asp Met

245 250 255

Gln Tyr Asp Pro Arg Tyr Thr Pro Gly His Leu Gln Arg Ser Leu Val

260 265 270

Asp Ala Gly Leu Leu Gly Lys Lys Asn Gly Arg Ser Tyr Phe Ala Ala

275 280 285

Glu Glu Asn Ala Leu Pro Val Thr Ala Ala Thr Asp Ala Asp Val Glu

290 295 300

Thr Leu Arg Val Tyr Gly Glu His Pro Phe Phe Thr Leu Leu Gln Gln

305 310 315 320

Arg Ala Ala Leu Gln Trp Pro Arg Leu Arg Val Glu Gln Arg Pro Ala

325 330 335

Leu Pro Gly Leu Gly Pro Ala Val Gln Ile Asn Glu Ala Phe Thr Val

340 345 350

Ser Val Thr Asp Gly Arg Thr Ala Ser Gln Leu Ala Glu Leu Thr Ala

355 360 365

Ala Asp Ala Phe Val Val Asp Val Ala Leu Asn Tyr Ala Asp Thr Ala

370 375 380

Tyr Leu Val Ala Ala His Asn Arg His Ala Ser Ala Ala Asn Lys Ala

385 390 395 400

Leu Phe Leu Arg Leu Leu His Thr Ala Ile Pro Gln Val Glu Phe Ile

405 410 415

Lys Asp Ser Pro Ala Leu Ile Val Ala Arg Val Leu Ser Ser Leu Ile

420 425 430

Asn Glu Ser Val Ile Met Val Glu Ser Gly Val Cys Ser Arg Glu Asp

435 440 445

Ile Asp Val Ala Ala Val Ala Gly Val Asn Tyr Ala Asp Gly Ile Phe

450 455 460

Gly Trp Leu Asp Ser Leu Gly Glu Lys Asn Val Arg Thr Thr Leu Ser

465 470 475 480

Asn Leu Ala Gln Leu Leu His Ala Ala Arg Tyr Ala Pro His Tyr Thr

485 490 495

Leu Leu His Ala Ala Gln Pro Ala Leu Thr Thr Thr Pro

500 505

<210> 51

<211> 509

<212> PRT

<213> Serratia marcescens 2880STDY5682884

<400> 51

Met Ala Glu Ser Asn Ala Ala Ile Gln Ser Ala Ala Ile Ile Gly Ala

1 5 10 15

Gly Thr Met Gly Arg Gly Ile Ala Tyr Leu Phe Ala Gln Lys Gly Ile

20 25 30

Arg Thr Val Leu Tyr Asn Arg Asn Gly Asn Thr Leu Asn Gln Ala Arg

35 40 45

Glu Tyr Ile Ala Gln Asp Leu Asn Lys Lys Val Glu Gln Gly Lys Ile

50 55 60

Ala Leu Gln Asp Lys Gly Ala Val Leu Ala Asn Leu Val Phe Thr Ser

65 70 75 80

Val Phe Glu Thr Ile Ala Asp Ser Glu Leu Val Ile Glu Thr Ile Ala

85 90 95

Glu Gln Glu Gln Thr Lys Leu Glu Val Leu Ala Ala Ile Ala Ala Ala

100 105 110

Val Lys Pro Asp Thr Leu Ile Ala Thr Asn Thr Ser Ser Leu Ser Leu

115 120 125

Asn Lys Leu Ala Thr Ala Val Thr His Ser Glu Arg Phe Ile Gly Leu

130 135 140

His Phe Phe Asn Pro Ala Pro Leu Met Lys Leu Ile Glu Ile Ile Pro

145 150 155 160

Ala Tyr Phe Thr Ala Arg Ala Thr Thr Glu Arg Cys Arg Gln Leu Val

165 170 175

Ala Ala Leu Gly Lys Arg Asp Val Val Cys Gln Ala Thr Pro Gly Phe

180 185 190

Ile Val Asn Arg Met Ala Arg Pro Tyr Tyr Leu Glu Gly Phe Arg Leu

195 200 205

Leu Glu Glu His Val Ala Arg Ala Pro Gln Ile Asp Arg Ala Leu Lys

210 215 220

Ala Gly Gly His Phe Arg Met Gly Pro Leu Glu Leu Thr Asp Phe Ile

225 230 235 240

Gly Gln Asp Ile Asn Tyr Gln Val Ser Arg Gln Ile Trp Gln Asp Met

245 250 255

Gln Tyr Asp Pro Arg Tyr Thr Pro Gly His Leu Gln Arg Ser Leu Val

260 265 270

Asp Ala Gly Leu Leu Gly Lys Lys Asn Gly Arg Ser Tyr Phe Ala Ala

275 280 285

Glu Glu Asn Ala Leu Pro Val Thr Ala Ala Thr Asp Ala Asp Val Glu

290 295 300

Thr Leu Arg Ile Tyr Gly Glu His Pro Phe Phe Thr Leu Leu Gln Gln

305 310 315 320

Arg Ala Thr Leu Gln Trp Pro Arg Leu Arg Val Glu Gln Arg Pro Ala

325 330 335

Leu Pro Gly Leu Gly Pro Ala Val Gln Ile Asn Glu Ala Phe Thr Val

340 345 350

Ser Val Thr Asp Gly Arg Thr Ala Ser Gln Leu Ala Glu Leu Met Ala

355 360 365

Ala Asp Ala Phe Val Val Asp Val Ala Leu Asn Tyr Ala Asp Thr Ala

370 375 380

Tyr Leu Val Ala Ala His Asn Arg His Ala Ser Ala Ala Asn Lys Ala

385 390 395 400

Leu Phe Leu Arg Leu Leu His Thr Ala Ile Pro Gln Val Glu Phe Ile

405 410 415

Lys Asp Ser Pro Ala Leu Ile Val Ala Arg Val Leu Ser Ser Leu Ile

420 425 430

Asn Glu Ser Val Ile Met Val Glu Ser Gly Val Cys Ser Arg Glu Asp

435 440 445

Ile Asp Val Ala Ala Val Ala Gly Val Asn Tyr Ala Asp Gly Ile Phe

450 455 460

Gly Trp Leu Asp Ser Leu Gly Glu Lys Asn Val Arg Met Thr Leu Ser

465 470 475 480

Asn Leu Ala Gln Leu Leu His Ala Ala Arg Tyr Ala Pro His Tyr Thr

485 490 495

Leu Leu His Ala Ala Gln Pro Ala Leu Thr Thr Thr Pro

500 505

<210> 52

<211> 509

<212> PRT

<213> Serratia marcescens D-3

<400> 52

Met Ala Glu Ser Asn Ala Ala Ile Gln Ser Ala Ala Ile Ile Gly Ala

1 5 10 15

Gly Thr Met Gly Arg Gly Ile Ala Tyr Leu Phe Ala Gln Lys Gly Ile

20 25 30

Arg Thr Val Leu Tyr Asn Arg Asn Gly Asn Thr Leu Asn Gln Ala Arg

35 40 45

Glu Tyr Ile Ala Gln Asp Leu Asn Lys Lys Val Glu Gln Gly Lys Ile

50 55 60

Ala Leu Gln Asp Lys Gly Ala Val Leu Ala Asn Leu Val Phe Thr Ser

65 70 75 80

Val Phe Glu Thr Ile Ala Asp Ser Asp Leu Val Ile Glu Thr Ile Ala

85 90 95

Glu Gln Glu Gln Thr Lys Leu Glu Val Leu Ala Ala Ile Ala Ala Ala

100 105 110

Val Lys Pro Asp Thr Leu Ile Ala Thr Asn Thr Ser Ser Leu Ser Leu

115 120 125

Asn Lys Leu Ala Thr Ala Val Thr His Ser Glu Arg Phe Ile Gly Leu

130 135 140

His Phe Phe Asn Pro Ala Pro Leu Met Lys Leu Ile Glu Ile Ile Pro

145 150 155 160

Ala Tyr Phe Thr Ala Arg Ala Thr Thr Glu Arg Cys Arg Gln Leu Val

165 170 175

Ala Ala Leu Gly Lys Arg Asp Val Val Cys Gln Ala Thr Pro Gly Phe

180 185 190

Ile Val Asn Arg Met Ala Arg Pro Tyr Tyr Leu Glu Gly Phe Arg Leu

195 200 205

Leu Glu Glu His Val Ala Arg Ala Pro Gln Ile Asp Arg Ala Leu Lys

210 215 220

Ala Gly Gly His Phe Arg Met Gly Pro Leu Glu Leu Thr Asp Phe Ile

225 230 235 240

Gly Gln Asp Ile Asn Tyr Gln Val Ser Arg Gln Ile Trp Gln Asp Met

245 250 255

Gln Tyr Asp Pro Arg Tyr Thr Pro Gly His Leu Gln Arg Ser Leu Val

260 265 270

Asp Ala Gly Leu Leu Gly Lys Lys Asn Gly Arg Ser Tyr Phe Ala Ala

275 280 285

Glu Glu Asn Ala Leu Pro Val Thr Ala Ala Ile Asp Ala Asp Val Glu

290 295 300

Thr Leu Arg Val Tyr Gly Glu His Pro Phe Phe Thr Leu Leu Gln Gln

305 310 315 320

Arg Ala Ala Leu Gln Trp Pro Arg Leu Arg Val Glu Gln Arg Pro Ala

325 330 335

Leu Pro Gly Leu Gly Pro Ala Val Gln Ile Asn Glu Ala Phe Thr Val

340 345 350

Ser Val Thr Asp Gly Arg Thr Ala Ser Gln Leu Ala Glu Leu Thr Ala

355 360 365

Ala Asp Ala Phe Val Val Asp Val Ala Leu Asn Tyr Ala Asp Thr Ala

370 375 380

Tyr Leu Val Ala Ala His Asn Arg His Ala Ser Ala Ala Asn Lys Ala

385 390 395 400

Leu Phe Leu Arg Leu Leu His Thr Ala Ile Pro Gln Val Glu Phe Ile

405 410 415

Lys Asp Ser Pro Ala Leu Ile Val Ala Arg Val Leu Ser Ser Leu Ile

420 425 430

Asn Glu Ser Val Ile Met Val Glu Ser Gly Val Cys Ser Arg Glu Asp

435 440 445

Ile Asp Val Ala Ala Val Ala Gly Val Asn Tyr Ala Asp Gly Ile Phe

450 455 460

Gly Leu Leu Asp Ser Leu Gly Glu Lys Asn Val Arg Thr Thr Leu Ser

465 470 475 480

Asn Leu Ala Gln Leu Leu His Ala Ala Arg Tyr Ala Pro His Tyr Thr

485 490 495

Leu Leu His Ala Ala Gln Pro Ala Leu Thr Thr Thr Pro

500 505

<210> 53

<211> 509

<212> PRT

<213> Serratia marcescens 2880STDY5682957

<400> 53

Met Ala Glu Ser Asn Ala Ala Ile Gln Ser Ala Ala Ile Ile Gly Ala

1 5 10 15

Gly Thr Met Gly Arg Gly Ile Ala Tyr Leu Phe Ala Gln Lys Gly Ile

20 25 30

Arg Thr Val Leu Tyr Asn Arg Asn Gly Asn Thr Leu Asn Gln Ala Arg

35 40 45

Glu Tyr Ile Glu Leu Asp Leu Asn Lys Lys Val Glu Gln Gly Lys Ile

50 55 60

Ala Leu Gln Asp Lys Gly Ala Val Leu Ala Asn Leu Val Phe Thr Ser

65 70 75 80

Ala Phe Glu Thr Ile Ala Asp Ser Glu Leu Val Ile Glu Thr Ile Ala

85 90 95

Glu Gln Glu Gln Thr Lys Leu Glu Val Leu Ala Ala Ile Ala Ala Ala

100 105 110

Val Lys Pro Asp Thr Leu Ile Ala Thr Asn Thr Ser Ser Leu Ser Leu

115 120 125

Asn Lys Leu Ala Thr Ala Val Thr His Ser Glu Arg Phe Ile Gly Leu

130 135 140

His Phe Phe Asn Pro Ala Pro Leu Met Lys Leu Ile Glu Ile Ile Pro

145 150 155 160

Ala Tyr Phe Thr Ala Arg Ala Thr Thr Glu Arg Cys Arg Gln Leu Val

165 170 175

Ala Ala Leu Gly Lys Arg Asp Val Val Cys Gln Ala Thr Pro Gly Phe

180 185 190

Ile Val Asn Arg Met Ala Arg Pro Tyr Tyr Leu Glu Gly Phe Arg Leu

195 200 205

Leu Glu Glu His Val Ala Arg Ala Pro Gln Ile Asp Arg Ala Leu Lys

210 215 220

Ala Gly Gly His Phe Arg Met Gly Pro Leu Glu Leu Thr Asp Phe Ile

225 230 235 240

Gly Gln Asp Ile Asn Tyr Gln Val Ser Arg Gln Ile Trp Gln Asp Met

245 250 255

Gln Tyr Asp Pro Arg Tyr Thr Pro Gly His Leu Gln Arg Ser Leu Val

260 265 270

Asp Ala Gly Leu Leu Gly Lys Lys Asn Gly Arg Ser Tyr Phe Ala Ala

275 280 285

Glu Glu Asn Ala Leu Pro Val Thr Ala Ala Thr Asp Ala Asp Val Glu

290 295 300

Thr Leu Arg Val Tyr Gly Glu His Pro Phe Phe Thr Leu Leu Gln Gln

305 310 315 320

Arg Ala Ala Leu Gln Trp Pro Arg Leu Arg Val Glu Gln Arg Pro Ala

325 330 335

Leu Pro Gly Leu Gly Pro Ala Val Gln Ile Asn Glu Ala Phe Thr Val

340 345 350

Ser Val Thr Asp Gly Arg Thr Ala Ser Gln Leu Ala Glu Leu Thr Ala

355 360 365

Ala Asp Ala Phe Val Val Asp Val Ala Leu Asn Tyr Ala Asp Thr Ala

370 375 380

Tyr Leu Val Ala Ala His Asn Arg His Ala Ser Ala Ala Asn Lys Ala

385 390 395 400

Leu Phe Leu Arg Leu Leu His Thr Ala Ile Pro Gln Val Glu Phe Ile

405 410 415

Lys Asp Ser Pro Ala Leu Ile Val Ala Arg Val Leu Ser Ser Leu Ile

420 425 430

Asn Glu Ser Val Ile Met Val Glu Ser Gly Val Cys Ser Arg Glu Asp

435 440 445

Ile Asp Val Ala Ala Val Ala Gly Val Asn Tyr Ala Asp Gly Ile Phe

450 455 460

Gly Trp Leu Asp Ser Leu Gly Glu Lys Asn Val Arg Thr Thr Leu Ser

465 470 475 480

Asn Leu Ala Gln Leu Leu His Ala Ala Arg Tyr Ala Pro His Tyr Thr

485 490 495

Leu Leu His Ala Ala Gln Pro Ala Leu Thr Thr Thr Pro

500 505

<210> 54

<211> 509

<212> PRT

<213> Serratia marcescens YDC563

<400> 54

Met Ala Glu Ser Asn Ala Ala Ile Gln Ser Ala Ala Ile Ile Gly Ala

1 5 10 15

Gly Thr Met Gly Arg Gly Ile Ala Tyr Leu Phe Ala Gln Lys Gly Ile

20 25 30

Arg Thr Val Leu Tyr Asn Arg Asn Gly Asn Thr Leu Asn Gln Ala Arg

35 40 45

Glu Tyr Ile Glu Leu Asp Leu Asn Lys Lys Val Glu Gln Gly Lys Ile

50 55 60

Ala Leu Gln Asp Lys Gly Ala Val Leu Ala Asn Leu Val Phe Thr Ser

65 70 75 80

Ala Phe Glu Thr Ile Ala Asp Ser Glu Leu Val Ile Glu Thr Ile Ala

85 90 95

Glu Gln Glu Gln Thr Lys Leu Glu Val Leu Ala Ala Ile Ala Ala Ala

100 105 110

Val Lys Pro Asp Thr Leu Ile Ala Thr Asn Thr Ser Ser Leu Ser Leu

115 120 125

Asn Lys Leu Ala Thr Ala Val Thr His Ser Glu Arg Phe Ile Gly Leu

130 135 140

His Phe Phe Asn Pro Ala Pro Leu Met Lys Leu Ile Glu Ile Ile Pro

145 150 155 160

Ala Tyr Phe Thr Ala Arg Ala Thr Thr Glu Arg Cys Arg Gln Leu Val

165 170 175

Ala Ala Leu Gly Lys Arg Asp Val Val Cys Gln Ala Thr Pro Gly Phe

180 185 190

Ile Val Asn Arg Met Ala Arg Pro Tyr Tyr Leu Glu Gly Phe Arg Leu

195 200 205

Leu Glu Glu His Val Ala Arg Ala Pro Gln Ile Asp Arg Ala Leu Lys

210 215 220

Ala Gly Gly His Phe Arg Met Gly Pro Leu Glu Leu Thr Asp Phe Ile

225 230 235 240

Gly Gln Asp Ile Asn Tyr Gln Val Ser Arg Gln Ile Trp Gln Asp Met

245 250 255

Gln Tyr Asp Pro Arg Tyr Thr Pro Gly His Leu Gln Arg Ser Leu Val

260 265 270

Asp Ala Gly Leu Leu Gly Lys Lys Asn Gly Arg Ser Tyr Phe Ala Ala

275 280 285

Glu Glu Asn Ala Leu Pro Val Thr Ala Ala Thr Asp Ala Asp Val Glu

290 295 300

Thr Leu Arg Val Tyr Gly Glu His Pro Phe Phe Thr Leu Leu Gln Gln

305 310 315 320

Arg Ala Ala Leu Gln Trp Pro Arg Leu Arg Val Glu Gln Arg Pro Ala

325 330 335

Leu Pro Gly Leu Gly Pro Ala Val Gln Ile Asn Glu Ala Phe Thr Val

340 345 350

Ser Val Thr Asp Gly Arg Thr Ala Ser Gln Leu Ala Glu Gln Thr Ala

355 360 365

Ala Asp Ala Phe Val Val Asp Val Ala Leu Asn Tyr Ala Asp Thr Ala

370 375 380

Tyr Leu Val Ala Ala His Asn Arg His Ala Ser Ala Ala Asn Lys Ala

385 390 395 400

Leu Phe Leu Arg Leu Leu His Thr Ala Ile Pro Gln Val Glu Phe Ile

405 410 415

Lys Asp Ser Pro Ala Leu Ile Val Ala Arg Val Leu Ser Ser Leu Ile

420 425 430

Asn Glu Ser Val Ile Met Val Glu Ser Gly Val Cys Ser Arg Glu Asp

435 440 445

Ile Asp Val Ala Ala Val Ala Gly Val Asn Tyr Ala Asp Gly Ile Phe

450 455 460

Gly Trp Leu Asp Ser Leu Gly Glu Lys Asn Val Arg Thr Thr Leu Ser

465 470 475 480

Asn Met Ala Gln Leu Leu His Ala Ala Arg Tyr Ala Pro His Tyr Thr

485 490 495

Leu Leu His Ala Ala Gln Pro Ala Leu Met Thr Thr Pro

500 505

<210> 55

<211> 509

<212> PRT

<213> Serratia marcescens 2880STDY5683035

<400> 55

Met Ala Glu Ser Asn Ala Ala Ile Gln Ser Ala Ala Ile Ile Gly Ala

1 5 10 15

Gly Thr Met Gly Arg Gly Ile Ala Tyr Leu Phe Ala Gln Lys Gly Ile

20 25 30

Arg Thr Val Leu Tyr Asn Arg Asn Gly Asn Thr Leu Asn Gln Ala Arg

35 40 45

Glu Tyr Ile Ala Gln Asp Leu Asn Lys Lys Val Glu Gln Gly Lys Ile

50 55 60

Ala Leu Gln Asp Lys Gly Ala Val Leu Ala Asn Leu Val Phe Thr Ser

65 70 75 80

Val Phe Glu Thr Ile Ala Asp Ser Asp Leu Val Ile Glu Thr Ile Ala

85 90 95

Glu Gln Glu Gln Thr Lys Leu Glu Val Leu Ala Ala Ile Ala Ala Ala

100 105 110

Val Lys Pro Asp Thr Leu Ile Ala Thr Asn Thr Ser Ser Leu Ser Leu

115 120 125

Asn Lys Leu Ala Thr Ala Val Thr His Ser Glu Arg Phe Ile Gly Leu

130 135 140

His Phe Phe Asn Pro Ala Pro Leu Met Lys Leu Ile Glu Ile Ile Pro

145 150 155 160

Ala Tyr Phe Thr Ala Arg Ala Thr Thr Glu Arg Cys Arg Gln Leu Val

165 170 175

Ala Ala Leu Gly Lys Arg Asp Val Val Cys Gln Ala Thr Pro Gly Phe

180 185 190

Ile Val Asn Arg Met Ala Arg Pro Tyr Tyr Leu Glu Gly Phe Arg Leu

195 200 205

Leu Glu Glu His Val Ala Arg Ala Pro Gln Ile Asp Arg Ala Leu Lys

210 215 220

Ala Gly Gly His Phe Arg Met Gly Pro Leu Glu Leu Thr Asp Phe Ile

225 230 235 240

Gly Gln Asp Ile Asn Tyr Gln Val Ser Arg Gln Ile Trp Gln Asp Met

245 250 255

Gln Tyr Asp Pro Arg Tyr Thr Pro Gly His Leu Gln Arg Ser Leu Val

260 265 270

Asp Ala Gly Leu Leu Gly Lys Lys Asn Gly Arg Ser Tyr Phe Ala Ala

275 280 285

Glu Glu Asn Ala Leu Pro Val Thr Ala Ala Ile Asp Ala Asp Val Glu

290 295 300

Thr Leu Arg Val Tyr Gly Glu His Pro Phe Phe Thr Leu Leu Gln Gln

305 310 315 320

Arg Ala Ala Leu Gln Trp Pro Arg Leu Arg Val Glu Gln Arg Pro Ala

325 330 335

Leu Pro Gly Leu Gly Pro Ala Val Gln Ile Asn Glu Ala Phe Thr Val

340 345 350

Ser Val Thr Asp Gly Arg Thr Ala Ser Gln Leu Ala Glu Leu Thr Ala

355 360 365

Ala Asp Val Phe Val Val Asp Val Ala Leu Asn Tyr Ala Asp Thr Ala

370 375 380

Tyr Leu Val Ala Ala His Asn Arg His Ala Ser Ala Ala Asn Lys Ala

385 390 395 400

Leu Phe Leu Arg Leu Leu His Thr Ala Ile Pro Gln Val Glu Phe Ile

405 410 415

Lys Asp Ser Pro Ala Leu Ile Val Ala Arg Val Leu Ser Ser Leu Ile

420 425 430

Asn Glu Ser Val Ile Met Val Glu Ser Gly Val Cys Ser Arg Glu Asp

435 440 445

Ile Asp Val Ala Ala Val Ala Gly Val Asn Tyr Ala Asp Gly Ile Phe

450 455 460

Gly Leu Leu Asp Ser Leu Gly Glu Lys Asn Val Arg Thr Thr Leu Ser

465 470 475 480

Asn Leu Ala Gln Leu Leu His Ala Ala Arg Tyr Ala Pro His Tyr Thr

485 490 495

Leu Leu His Ala Ala Gln Pro Ala Leu Thr Thr Thr Pro

500 505

<210> 56

<211> 509

<212> PRT

<213> Serratia marcescens 2880STDY5682930

<400> 56

Met Ala Glu Ser Asn Ala Ala Ile Gln Ser Ala Ala Ile Ile Gly Ala

1 5 10 15

Gly Thr Met Gly Arg Gly Ile Ala Tyr Leu Phe Ala Gln Lys Gly Ile

20 25 30

Arg Thr Val Leu Tyr Asn Arg Asn Gly Asn Thr Leu Asn Gln Ala Arg

35 40 45

Glu Tyr Ile Ala Gln Asp Leu Asn Lys Lys Val Glu Gln Gly Lys Ile

50 55 60

Ala Leu Gln Asp Lys Gly Ala Val Leu Ala Asn Leu Val Phe Thr Ser

65 70 75 80

Val Phe Glu Thr Ile Ala Asp Ser Asp Leu Val Ile Glu Thr Ile Ala

85 90 95

Glu Gln Glu Gln Thr Lys Leu Glu Val Leu Ala Ala Ile Ala Ala Ala

100 105 110

Val Lys Pro Asp Thr Leu Ile Ala Thr Asn Thr Ser Ser Leu Ser Leu

115 120 125

Asn Lys Leu Ala Thr Ala Val Thr His Ser Glu Arg Phe Ile Gly Leu

130 135 140

His Phe Phe Asn Pro Ala Pro Leu Met Lys Leu Ile Glu Ile Ile Pro

145 150 155 160

Ala Tyr Phe Thr Ala Arg Ala Thr Thr Glu Arg Cys Arg Gln Leu Val

165 170 175

Ala Ala Leu Gly Lys Arg Asp Val Val Cys Gln Ala Thr Pro Gly Phe

180 185 190

Ile Val Asn Arg Met Ala Arg Pro Tyr Tyr Leu Glu Gly Phe Arg Leu

195 200 205

Leu Glu Glu His Val Ala Arg Ala Pro Gln Ile Asp Arg Ala Leu Lys

210 215 220

Ala Gly Gly His Phe Arg Met Gly Pro Leu Glu Leu Thr Asp Phe Ile

225 230 235 240

Gly Gln Asp Ile Asn Tyr Gln Val Ser Arg Gln Ile Trp Gln Asp Met

245 250 255

Gln Tyr Asp Pro Arg Tyr Thr Pro Gly His Leu Gln Arg Ser Leu Val

260 265 270

Asp Ala Gly Leu Leu Gly Lys Lys Asn Gly Arg Ser Tyr Phe Ala Ala

275 280 285

Glu Glu Asn Ala Leu Pro Val Thr Ala Ala Thr Asp Ala Asp Val Glu

290 295 300

Thr Leu Arg Val Tyr Gly Glu His Pro Phe Phe Thr Leu Leu Gln Gln

305 310 315 320

Arg Ala Ala Leu Gln Trp Pro Arg Leu Arg Val Glu Gln Arg Pro Ala

325 330 335

Leu Pro Gly Leu Gly Pro Ala Val Gln Ile Asn Glu Ala Phe Thr Val

340 345 350

Ser Val Thr Asp Gly Arg Thr Ala Ser Gln Leu Ala Glu Leu Thr Ala

355 360 365

Ala Asp Ala Phe Val Val Asp Val Ala Leu Asn Tyr Ala Asp Thr Ala

370 375 380

Tyr Leu Val Ala Ala His Asn Arg His Ala Ser Ala Ala Asn Lys Ala

385 390 395 400

Leu Phe Leu Arg Leu Leu His Thr Ala Ile Pro Gln Val Glu Phe Ile

405 410 415

Lys Asp Ser Pro Ala Leu Ile Val Ala Arg Val Leu Ser Ser Leu Ile

420 425 430

Asn Glu Ser Val Ile Met Val Glu Ser Gly Val Cys Ser Arg Glu Asp

435 440 445

Ile Asn Val Ala Ala Val Ala Gly Val Asn Tyr Ala Asp Gly Ile Phe

450 455 460

Gly Leu Leu Asp Ser Leu Gly Glu Lys Asn Val Arg Thr Thr Leu Ser

465 470 475 480

Asn Leu Ala Gln Leu Leu His Ala Ala Arg Tyr Ala Pro His Tyr Thr

485 490 495

Leu Leu His Ala Ala Gln Pro Val Leu Thr Thr Thr Pro

500 505

<210> 57

<211> 509

<212> PRT

<213> Serratia marcescens 790

<400> 57

Met Ala Glu Ser Asn Ala Ala Ile Gln Ser Ala Ala Ile Ile Gly Ala

1 5 10 15

Gly Thr Met Gly Arg Gly Ile Ala Tyr Leu Phe Ala Gln Lys Gly Ile

20 25 30

Arg Thr Val Leu Tyr Asn Arg Asn Gly Asn Thr Leu Asn Gln Ala Arg

35 40 45

Glu Tyr Ile Ala Gln Asp Leu Asn Lys Lys Val Glu Gln Gly Lys Ile

50 55 60

Ala Leu Arg Asp Lys Gly Ala Val Leu Ala Asn Leu Val Phe Thr Ser

65 70 75 80

Val Phe Glu Thr Ile Ala Asp Ser Glu Leu Val Ile Glu Thr Ile Ala

85 90 95

Glu Gln Glu Gln Thr Lys Leu Glu Val Leu Ala Ala Ile Ala Ala Ala

100 105 110

Val Lys Pro Asp Thr Leu Ile Ala Thr Asn Thr Ser Ser Leu Ser Leu

115 120 125

Asn Lys Leu Ala Thr Ala Val Thr His Ser Glu Arg Phe Ile Gly Leu

130 135 140

His Phe Phe Asn Pro Ala Pro Leu Met Lys Leu Ile Glu Ile Ile Pro

145 150 155 160

Ala Tyr Phe Thr Ala Arg Ala Thr Thr Glu Arg Cys Arg Gln Leu Val

165 170 175

Ala Ala Leu Gly Lys Arg Asp Val Val Cys Gln Ala Thr Pro Gly Phe

180 185 190

Ile Val Asn Arg Met Ala Arg Pro Tyr Tyr Leu Glu Gly Phe Arg Leu

195 200 205

Leu Glu Glu His Val Ala Arg Ala Pro Gln Ile Asp Arg Ala Leu Lys

210 215 220

Ala Gly Gly His Phe Arg Met Gly Pro Leu Glu Leu Thr Asp Phe Ile

225 230 235 240

Gly Gln Asp Ile Asn Tyr Gln Val Ser Arg Gln Ile Trp Gln Asp Met

245 250 255

Gln Tyr Asp Pro Arg Tyr Thr Pro Gly His Leu Gln Arg Ser Leu Val

260 265 270

Asp Ala Gly Leu Leu Gly Lys Lys Asn Gly Arg Ser Tyr Phe Ala Ala

275 280 285

Glu Glu Asn Ala Leu Pro Val Thr Ala Ala Thr Asp Ala Asp Val Glu

290 295 300

Thr Leu Arg Val Tyr Gly Glu His Pro Phe Phe Thr Leu Leu Gln Gln

305 310 315 320

Arg Ala Ala Leu Gln Trp Pro Arg Leu Arg Val Glu Gln Arg Pro Ala

325 330 335

Leu Pro Gly Leu Gly Pro Ala Val Gln Thr Asn Glu Ala Phe Thr Val

340 345 350

Ser Val Thr Asp Gly Arg Thr Ala Asn Gln Leu Ala Glu Leu Thr Ala

355 360 365

Ala Asp Ala Phe Val Val Asp Val Ala Leu Asn Tyr Ala Asp Thr Ala

370 375 380

Tyr Leu Val Ala Ala His Asn Arg His Ala Ser Ala Ala Asn Lys Ala

385 390 395 400

Leu Phe Leu Arg Leu Leu His Thr Ala Ile Pro Gln Val Glu Phe Ile

405 410 415

Lys Asp Ser Pro Ala Leu Ile Val Ala Arg Val Leu Ser Ser Leu Ile

420 425 430

Asn Glu Ser Val Ile Met Val Glu Ser Gly Val Cys Ser Arg Glu Asp

435 440 445

Ile Asp Val Ala Ala Val Ala Gly Val Asn Tyr Ala Asp Gly Ile Phe

450 455 460

Gly Trp Leu Asp Ser Leu Gly Glu Lys Asn Val Arg Thr Thr Leu Ser

465 470 475 480

Asn Met Ala Gln Leu Leu His Ala Ala Arg Tyr Ala Pro His Tyr Thr

485 490 495

Leu Leu His Ala Ala Gln Pro Ala Leu Met Thr Thr Pro

500 505

<210> 58

<211> 509

<212> PRT

<213> Serratia marcescens UMH5

<400> 58

Met Ala Glu Ser Asn Ala Ala Ile Gln Ser Ala Ala Ile Ile Gly Ala

1 5 10 15

Gly Thr Met Gly Arg Gly Ile Ala Tyr Leu Phe Ala Gln Lys Gly Ile

20 25 30

Arg Thr Val Leu Tyr Asn Arg Asn Gly Asn Thr Leu Ser Gln Ala Arg

35 40 45

Asp Tyr Ile Glu Leu Asp Leu Asn Lys Lys Val Glu Gln Gly Lys Ile

50 55 60

Ala Leu Gln Asp Lys Gly Ala Val Leu Ala Asn Leu Val Phe Thr Ser

65 70 75 80

Val Phe Glu Thr Ile Ala Asp Ser Glu Leu Val Ile Glu Thr Ile Ala

85 90 95

Glu Gln Glu Gln Thr Lys Leu Glu Val Leu Ala Thr Ile Ala Ala Ala

100 105 110

Val Lys Pro Asp Thr Leu Ile Ala Thr Asn Thr Ser Ser Leu Ser Leu

115 120 125

Asn Lys Leu Ala Thr Ala Val Thr His Ser Glu Arg Phe Ile Gly Leu

130 135 140

His Phe Phe Asn Pro Ala Pro Leu Met Lys Leu Ile Glu Ile Ile Pro

145 150 155 160

Ala Tyr Cys Thr Ala His Ala Thr Thr Glu Arg Cys Arg Gln Leu Val

165 170 175

Ala Ala Leu Gly Lys Arg Asp Val Val Cys Gln Ala Thr Pro Gly Phe

180 185 190

Ile Val Asn Arg Met Ala Arg Pro Tyr Tyr Leu Glu Gly Phe Arg Leu

195 200 205

Leu Glu Glu His Val Ala Arg Ala Pro Gln Ile Asp Arg Ala Leu Lys

210 215 220

Ala Gly Gly His Phe Arg Met Gly Pro Leu Glu Leu Thr Asp Phe Ile

225 230 235 240

Gly Gln Asp Ile Asn Tyr Gln Val Ser Arg Gln Ile Trp Gln Asp Met

245 250 255

Gln Tyr Asp Ala Arg Tyr Thr Pro Gly His Leu Gln Arg Ser Leu Val

260 265 270

Asp Ala Gly Leu Leu Gly Lys Lys Asn Gly Arg Ser Tyr Phe Ala Val

275 280 285

Glu Glu Asn Ala Pro Pro Val Met Ala Ala Thr Asp Ala Asp Ile Glu

290 295 300

Thr Leu His Val Tyr Gly Glu His Pro Phe Phe Thr Leu Leu Gln Gln

305 310 315 320

Arg Ala Ala Leu Gln Trp Pro Arg Leu Arg Val Glu Gln Arg Gln Ala

325 330 335

Leu Pro Gly Leu Gly Pro Ala Val Arg Ile Asn Asp Ala Phe Thr Ile

340 345 350

Ser Ile Thr Asp Gly Arg Thr Ala Asn Gln Leu Ala Glu Gln Thr Ala

355 360 365

Ala Asp Ala Phe Val Val Asp Val Ala Leu Asn Tyr Ala Asp Thr Ala

370 375 380

Tyr Leu Val Ala Ala His Ser Arg His Ala Ser Gly Ala Asn Lys Ala

385 390 395 400

Leu Phe Leu Arg Leu Leu His Thr Ala Ile Pro Gln Val Glu Phe Ile

405 410 415

Lys Asp Ser Pro Ala Leu Ile Val Ala Arg Val Leu Ser Ser Leu Ile

420 425 430

Asn Glu Ser Val Ile Met Val Glu Ser Gly Val Cys Ser Arg Glu Asp

435 440 445

Ile Asp Val Ala Ala Val Ala Gly Val Asn Tyr Ala Asp Gly Ile Phe

450 455 460

Gly Trp Leu Asp Ser Leu Gly Glu Lys Asn Val Arg Thr Thr Leu Ser

465 470 475 480

Asn Leu Ala Gln Leu Leu His Ala Ala Arg Tyr Ala Pro His Tyr Thr

485 490 495

Leu Leu His Ala Ala Gln Pro Ala Leu Thr Thr Thr Pro

500 505

<210> 59

<211> 509

<212> PRT

<213> Serratia marcescens 2880STDY5682988

<400> 59

Met Ala Glu Ser Asn Ala Ala Ile Gln Ser Ala Ala Ile Ile Gly Ala

1 5 10 15

Gly Thr Met Gly Arg Gly Ile Ala Tyr Leu Phe Ala Gln Lys Gly Ile

20 25 30

Arg Thr Val Leu Tyr Asn Arg Asn Gly Asn Thr Leu Ser Gln Ala Arg

35 40 45

Asp Tyr Ile Glu Leu Asp Leu Asn Lys Lys Val Glu Gln Gly Lys Ile

50 55 60

Ala Leu Gln Asp Lys Gly Ala Val Leu Ala Asn Leu Val Phe Thr Ser

65 70 75 80

Val Phe Glu Thr Ile Ala Asp Ser Glu Leu Val Ile Glu Thr Ile Ala

85 90 95

Glu Gln Glu Gln Thr Lys Leu Glu Val Leu Ala Thr Ile Ala Ala Ala

100 105 110

Val Lys Pro Asp Thr Leu Ile Ala Thr Asn Thr Ser Ser Leu Ser Leu

115 120 125

Asn Lys Leu Ala Thr Ala Val Thr His Ser Glu Arg Phe Ile Gly Leu

130 135 140

His Phe Phe Asn Pro Ala Pro Leu Met Lys Leu Ile Glu Ile Ile Pro

145 150 155 160

Ala Tyr Cys Thr Ala His Ala Thr Thr Glu Arg Cys Arg Gln Leu Val

165 170 175

Ala Ala Leu Gly Lys Arg Asp Val Val Cys Gln Ala Thr Pro Gly Phe

180 185 190

Ile Val Asn Arg Met Ala Arg Pro Tyr Tyr Leu Glu Gly Phe Arg Leu

195 200 205

Leu Glu Glu His Val Ala Arg Ala Pro Gln Ile Asp Arg Ala Leu Lys

210 215 220

Ala Gly Gly His Phe Arg Met Gly Pro Leu Glu Leu Thr Asp Phe Ile

225 230 235 240

Gly Gln Asp Ile Asn Tyr Gln Val Ser Arg Gln Ile Trp Gln Asp Met

245 250 255

Gln Tyr Asp Ala Arg Tyr Thr Pro Gly His Leu Gln Arg Ser Leu Val

260 265 270

Asp Ala Gly Leu Leu Gly Lys Lys Asn Gly Arg Ser Tyr Phe Ala Val

275 280 285

Glu Glu Asn Ala Pro Pro Val Met Ala Ala Thr Asp Ala Asp Ile Glu

290 295 300

Thr Leu His Val Tyr Gly Glu His Pro Phe Phe Thr Leu Leu Gln Gln

305 310 315 320

Arg Ala Ala Leu Gln Trp Pro Arg Leu Arg Val Glu Gln Arg Gln Ala

325 330 335

Leu Pro Gly Leu Gly Pro Ala Val Arg Ile Asn Asp Ala Phe Thr Ile

340 345 350

Ser Ile Thr Asp Gly Arg Thr Ala Asn Gln Leu Ala Glu Gln Thr Ala

355 360 365

Thr Asp Ala Phe Val Val Asp Val Ala Leu Asn Tyr Ala Asp Thr Ala

370 375 380

Tyr Leu Val Ala Ala His Ser Arg His Ala Ser Gly Ala Asn Lys Ala

385 390 395 400

Leu Phe Leu Arg Leu Leu His Thr Ala Ile Pro Gln Val Glu Phe Ile

405 410 415

Lys Asp Ser Pro Ala Leu Ile Val Ala Arg Val Leu Ser Ser Leu Ile

420 425 430

Asn Glu Ser Val Ile Met Val Glu Ser Gly Val Cys Ser Arg Glu Asp

435 440 445

Ile Asp Val Ala Ala Val Ala Gly Val Asn Tyr Ala Asp Gly Ile Phe

450 455 460

Gly Trp Leu Asp Ser Leu Gly Glu Lys Asn Val Arg Thr Thr Leu Ser

465 470 475 480

Asn Leu Ala Gln Leu Leu His Ala Ala Arg Tyr Ala Pro His Tyr Thr

485 490 495

Leu Leu His Ala Ala Gln Pro Ala Leu Thr Thr Thr Pro

500 505

<210> 60

<211> 509

<212> PRT

<213> Serratia marcescens 945154301

<400> 60

Met Ala Glu Ser Asn Ala Ala Ile Gln Ser Ala Ala Ile Ile Gly Ala

1 5 10 15

Gly Thr Met Gly Arg Gly Ile Ala Tyr Leu Phe Ala Gln Lys Gly Ile

20 25 30

Arg Thr Val Leu Tyr Asn Arg Asn Gly Asn Thr Leu Asn Gln Ala Arg

35 40 45

Asp Tyr Ile Glu Leu Asp Leu Asn Lys Lys Val Glu Gln Gly Lys Ile

50 55 60

Ala Leu Gln Asp Lys Gly Ala Val Leu Ala Asn Leu Val Phe Thr Ser

65 70 75 80

Val Phe Glu Thr Ile Ala Asp Ser Glu Leu Val Ile Glu Thr Ile Ala

85 90 95

Glu Gln Glu Gln Thr Lys Leu Glu Val Leu Ala Ala Ile Ala Ala Ala

100 105 110

Val Lys Pro Asp Thr Leu Ile Ala Thr Asn Thr Ser Ser Leu Ser Leu

115 120 125

Asn Lys Leu Ala Thr Ala Val Thr His Ser Glu Arg Phe Ile Gly Leu

130 135 140

His Phe Phe Asn Pro Ala Pro Leu Met Lys Leu Ile Glu Ile Ile Pro

145 150 155 160

Ala Tyr Phe Thr Ala His Ala Thr Thr Glu Arg Cys Arg Gln Leu Val

165 170 175

Ala Ala Leu Gly Lys Arg Asp Val Val Cys Gln Ala Thr Pro Gly Phe

180 185 190

Ile Val Asn Arg Met Ala Arg Pro Tyr Tyr Leu Glu Gly Phe Arg Leu

195 200 205

Leu Glu Glu His Val Ala Arg Ala Pro Gln Ile Asp Arg Ala Leu Lys

210 215 220

Ala Gly Gly His Phe Arg Met Gly Pro Leu Glu Leu Thr Asp Phe Ile

225 230 235 240

Gly Gln Asp Ile Asn Tyr Gln Val Ser Arg Gln Ile Trp Gln Asp Met

245 250 255

Gln Tyr Asp Ala Arg Tyr Thr Pro Gly His Leu Gln Arg Ser Leu Val

260 265 270

Asp Ala Gly Leu Leu Gly Lys Lys Asn Gly Arg Ser Tyr Phe Ala Ala

275 280 285

Glu Glu Asn Ala Pro Pro Val Met Ala Ala Thr Asp Ala Asp Ile Glu

290 295 300

Thr Leu His Val Tyr Gly Glu His Pro Phe Phe Thr Leu Leu Gln Gln

305 310 315 320

Arg Ala Ala Leu Gln Trp Pro Gln Leu Arg Val Glu Gln Arg Pro Ala

325 330 335

Leu Pro Gly Leu Gly Pro Ala Val Arg Ile Asn Asp Ala Phe Ser Val

340 345 350

Ser Ile Thr Asp Gly Arg Thr Ala Asn Gln Leu Ala Glu Gln Thr Ala

355 360 365

Ala Asp Ala Phe Val Val Asp Val Ala Leu Asn Tyr Ala Asp Thr Ala

370 375 380

Tyr Leu Val Ala Ala His Ser Arg His Ala Ser Ala Ala Asn Lys Ala

385 390 395 400

Leu Phe Leu Arg Leu Leu His Thr Ala Ile Pro Gln Val Glu Phe Ile

405 410 415

Lys Asp Ser Pro Ala Leu Ile Val Ala Arg Val Leu Ser Ser Leu Ile

420 425 430

Asn Glu Ser Val Ile Met Val Glu Ser Gly Val Cys Ser Arg Glu Asp

435 440 445

Ile Asp Val Ala Ala Val Ala Gly Val Asn Tyr Ala Asp Gly Ile Phe

450 455 460

Gly Trp Leu Asp Ser Leu Gly Glu Lys Asn Val Arg Thr Thr Leu Ser

465 470 475 480

Asn Leu Ala Gln Leu Leu His Ala Ala Arg Tyr Ala Pro His Tyr Thr

485 490 495

Leu Leu His Ala Ala Gln Pro Ala Leu Thr Thr Thr Pro

500 505

<210> 61

<211> 509

<212> PRT

<213> Serratia marcescens at10508

<400> 61

Met Ala Glu Ser Asn Ala Ala Ile Gln Ser Ala Ala Ile Ile Gly Ala

1 5 10 15

Gly Thr Met Gly Arg Gly Ile Ala Tyr Leu Phe Ala Gln Lys Gly Ile

20 25 30

Arg Thr Val Leu Tyr Asn Arg Asn Gly Asn Thr Leu Asn Gln Ala Arg

35 40 45

Asp Tyr Ile Glu Leu Asp Leu Asn Lys Lys Val Glu Gln Gly Lys Ile

50 55 60

Ala Leu Gln Asp Lys Gly Ala Val Leu Ala Asn Leu Val Phe Thr Ser

65 70 75 80

Val Phe Glu Thr Ile Ala Asp Ser Glu Leu Val Ile Glu Thr Ile Ala

85 90 95

Glu Gln Glu Gln Thr Lys Leu Glu Val Leu Ala Ala Ile Ala Ala Ala

100 105 110

Val Lys Pro Asp Thr Leu Ile Ala Thr Asn Thr Ser Ser Leu Ser Leu

115 120 125

Asn Lys Leu Ala Thr Ala Val Thr His Ser Glu Arg Phe Ile Gly Leu

130 135 140

His Phe Phe Asn Pro Ala Pro Leu Met Lys Leu Ile Glu Ile Ile Pro

145 150 155 160

Ala Tyr Phe Thr Ala His Ala Thr Thr Glu Arg Cys Arg Gln Leu Val

165 170 175

Thr Ala Leu Gly Lys Arg Asp Val Val Cys Gln Ala Thr Pro Gly Phe

180 185 190

Ile Val Asn Arg Met Ala Arg Pro Tyr Tyr Leu Glu Gly Phe Arg Leu

195 200 205

Leu Glu Glu His Val Ala Arg Ala Pro Gln Ile Asp Arg Ala Leu Lys

210 215 220

Ala Gly Gly His Phe Arg Met Gly Pro Leu Glu Leu Thr Asp Phe Ile

225 230 235 240

Gly Gln Asp Ile Asn Tyr Gln Val Ser Arg Gln Ile Trp Gln Asp Met

245 250 255

Gln Tyr Asp Ala Arg Tyr Thr Pro Gly His Leu Gln Arg Ser Leu Val

260 265 270

Asp Ala Gly Leu Leu Gly Lys Lys Asn Gly Arg Ser Tyr Phe Ala Val

275 280 285

Glu Glu Asn Ala Pro Pro Val Met Ala Ala Thr Asp Ala Asp Ile Glu

290 295 300

Thr Leu His Val Tyr Gly Glu His Pro Phe Phe Thr Leu Leu Gln Gln

305 310 315 320

Arg Ala Ala Leu Gln Trp Pro Gln Leu Arg Val Glu Gln Arg Pro Ala

325 330 335

Leu Pro Gly Leu Gly Pro Ala Val Arg Ile Asn Asp Ala Phe Thr Val

340 345 350

Ser Ile Thr Asp Gly Arg Thr Ala Asn Gln Leu Ala Glu Gln Thr Ala

355 360 365

Ala Asp Ala Phe Val Val Asp Val Ala Leu Asn Tyr Ala Asp Thr Ala

370 375 380

Tyr Leu Val Ala Ala His Ser Arg His Ala Ser Ala Ala Asn Lys Ala

385 390 395 400

Leu Phe Leu Arg Leu Leu His Thr Ala Ile Pro Gln Val Glu Phe Ile

405 410 415

Lys Asp Ser Pro Ala Leu Ile Val Ala Arg Val Leu Ser Ser Leu Ile

420 425 430

Asn Glu Ser Val Ile Met Val Glu Ser Gly Val Cys Ser Arg Glu Asp

435 440 445

Ile Asp Val Ala Ala Val Ala Gly Val Asn Tyr Ala Asp Gly Ile Phe

450 455 460

Gly Trp Leu Asp Ser Leu Gly Glu Lys Asn Val Arg Thr Thr Leu Ser

465 470 475 480

Asn Leu Ala Gln Leu Leu His Ala Ala Arg Tyr Ala Pro His Tyr Thr

485 490 495

Leu Leu His Ala Ala Gln Pro Ala Leu Thr Thr Thr Pro

500 505

<210> 62

<211> 509

<212> PRT

<213> Serratia marcescens ML2637

<400> 62

Met Ala Glu Ser Asn Ala Ala Ile Gln Ser Ala Ala Ile Ile Gly Ala

1 5 10 15

Gly Thr Met Gly Arg Gly Ile Ala Tyr Leu Phe Ala Gln Lys Gly Ile

20 25 30

Arg Thr Val Leu Tyr Asn Arg Asn Gly Asn Thr Leu Asn Gln Ala Arg

35 40 45

Asp Tyr Ile Glu Leu Asp Leu Asn Lys Lys Val Glu Gln Gly Lys Ile

50 55 60

Ala Leu Gln Asp Lys Gly Ala Val Leu Ala Asn Leu Val Phe Thr Ser

65 70 75 80

Val Phe Glu Thr Ile Ala Asp Ser Glu Leu Val Ile Glu Thr Ile Ala

85 90 95

Glu Gln Glu Gln Thr Lys Leu Glu Val Leu Ala Ala Ile Ala Ala Ala

100 105 110

Val Lys Pro Asp Thr Leu Ile Ala Thr Asn Thr Ser Ser Leu Ser Leu

115 120 125

Asn Lys Leu Ala Thr Ala Val Thr His Ser Glu Arg Phe Ile Gly Leu

130 135 140

His Phe Phe Asn Pro Ala Pro Leu Met Lys Leu Ile Glu Ile Ile Pro

145 150 155 160

Ala Tyr Phe Thr Ala His Ala Thr Thr Glu Arg Cys Arg Gln Leu Val

165 170 175

Thr Ala Leu Gly Lys Arg Asp Val Val Cys Gln Ala Thr Pro Gly Phe

180 185 190

Ile Val Asn Arg Met Ala Arg Pro Tyr Tyr Leu Glu Gly Phe Arg Leu

195 200 205

Leu Glu Glu His Val Ala Arg Ala Pro Gln Ile Asp Arg Ala Leu Lys

210 215 220

Ala Gly Gly His Phe Arg Met Gly Pro Leu Glu Leu Thr Asp Phe Ile

225 230 235 240

Gly Gln Asp Ile Asn Tyr Gln Val Ser Arg Gln Ile Trp Gln Asp Met

245 250 255

Gln Tyr Asp Ala Arg Tyr Thr Pro Gly His Leu Gln Arg Ser Leu Val

260 265 270

Asp Ala Gly Leu Leu Gly Lys Lys Asn Gly Arg Ser Tyr Phe Ala Val

275 280 285

Glu Glu Asn Ala Pro Pro Val Met Ala Ala Thr Asp Ala Asp Ile Glu

290 295 300

Thr Leu His Val Tyr Gly Glu His Pro Phe Phe Thr Leu Leu Gln Gln

305 310 315 320

Arg Ala Ala Leu Gln Trp Pro Gln Leu Arg Val Glu Gln Arg Pro Ala

325 330 335

Leu Pro Gly Leu Gly Pro Ala Val Arg Ile Asn Asp Ala Phe Thr Val

340 345 350

Ser Ile Thr Asp Gly Arg Thr Ala Asn Gln Leu Ala Glu Gln Thr Ala

355 360 365

Ala Asp Ala Phe Val Val Asp Val Ala Leu Asn Tyr Ala Asp Thr Ala

370 375 380

Tyr Leu Val Ala Ala His Ser Arg His Ala Ser Ala Ala Asn Lys Ala

385 390 395 400

Leu Phe Leu Arg Leu Leu His Thr Ala Ile Pro Gln Val Glu Phe Ile

405 410 415

Lys Asp Ser Pro Ala Leu Ile Val Ala Arg Val Leu Ser Ser Leu Ile

420 425 430

Asn Glu Ser Val Ile Met Val Glu Ser Gly Val Cys Ser Arg Glu Asp

435 440 445

Ile Asp Val Ala Ala Val Ala Gly Val Asn Tyr Ala Asp Gly Ile Phe

450 455 460

Gly Trp Leu Asp Ser Leu Gly Glu Lys Asn Val Arg Thr Thr Leu Ser

465 470 475 480

Asn Leu Ala Gln Leu Leu His Ala Ala Arg Tyr Ala Pro His Tyr Thr

485 490 495

Leu Leu His Ala Val Gln Pro Ala Leu Thr Thr Thr Pro

500 505

<210> 63

<211> 509

<212> PRT

<213> Serratia marcescens SM1978

<400> 63

Met Ala Glu Ser Asn Ala Ala Ile Gln Ser Ala Ala Ile Ile Gly Ala

1 5 10 15

Gly Thr Met Gly Arg Gly Ile Ala Tyr Leu Phe Ala Gln Lys Gly Ile

20 25 30

Arg Thr Val Leu Tyr Asn Arg Asn Gly Asn Thr Leu Asn Gln Ala Arg

35 40 45

Asp Tyr Ile Glu Leu Asp Leu Asn Lys Lys Val Glu Gln Gly Lys Ile

50 55 60

Ala Leu Gln Asp Lys Gly Ala Val Leu Ala Asn Leu Val Phe Thr Ser

65 70 75 80

Val Phe Glu Thr Ile Ala Asp Ser Glu Leu Val Ile Glu Thr Ile Ala

85 90 95

Glu Gln Glu Gln Thr Lys Leu Glu Val Leu Ala Ala Ile Ala Ala Ala

100 105 110

Val Lys Pro Asp Thr Leu Ile Ala Thr Asn Thr Ser Ser Leu Ser Leu

115 120 125

Asn Lys Leu Ala Thr Ala Val Thr His Ser Glu Arg Phe Ile Gly Leu

130 135 140

His Phe Phe Asn Pro Ala Pro Leu Met Lys Leu Ile Glu Ile Ile Pro

145 150 155 160

Ala Tyr Phe Thr Ala His Ala Thr Thr Glu Arg Cys Arg Gln Leu Val

165 170 175

Thr Ala Leu Gly Lys Arg Asp Val Val Cys Gln Ala Thr Pro Gly Phe

180 185 190

Ile Val Asn Arg Met Ala Arg Pro Tyr Tyr Leu Glu Gly Phe Arg Leu

195 200 205

Leu Glu Glu His Val Ala Arg Ala Pro Gln Ile Asp Arg Ala Leu Lys

210 215 220

Ala Gly Gly His Phe Arg Met Gly Pro Leu Glu Leu Thr Asp Phe Ile

225 230 235 240

Gly Gln Asp Ile Asn Tyr Gln Val Ser Arg Gln Ile Trp Gln Asp Met

245 250 255

Gln Tyr Asp Ala Arg Tyr Thr Pro Gly His Leu Gln Arg Ser Leu Val

260 265 270

Asp Ala Gly Leu Leu Gly Lys Lys Asn Gly Arg Ser Tyr Phe Ala Ala

275 280 285

Glu Glu Asn Ala Pro Pro Val Met Ala Ser Thr Asp Ala Asp Ile Glu

290 295 300

Thr Leu His Val Tyr Gly Glu His Pro Phe Phe Thr Leu Leu Gln Gln

305 310 315 320

Arg Ala Ala Leu Gln Trp Pro Gln Leu Arg Val Glu Gln Arg Pro Ala

325 330 335

Leu Pro Gly Leu Gly Pro Ala Val Arg Ile Asn Asp Ala Phe Ser Val

340 345 350

Ser Ile Thr Asp Gly Arg Thr Ala Asn Gln Leu Ala Glu Gln Thr Thr

355 360 365

Ala Asp Ala Phe Val Val Asp Val Ala Leu Asn Tyr Ala Asp Thr Ala

370 375 380

Tyr Leu Val Ala Ala His Ser Arg His Ala Ser Ala Ala Asn Lys Ala

385 390 395 400

Leu Phe Leu Arg Leu Leu His Thr Ala Ile Pro Gln Val Glu Phe Ile

405 410 415

Lys Asp Ser Pro Ala Leu Ile Val Ala Arg Val Leu Ser Ser Leu Ile

420 425 430

Asn Glu Ser Val Ile Met Val Glu Ser Gly Val Cys Ser Arg Glu Asp

435 440 445

Ile Asp Val Ala Ala Val Ala Gly Val Asn Tyr Ala Asp Gly Ile Phe

450 455 460

Gly Trp Leu Asp Ser Leu Gly Glu Lys Asn Val Arg Thr Thr Leu Ser

465 470 475 480

Asn Leu Ala Gln Leu Leu His Ala Ala Arg Tyr Ala Pro His Tyr Thr

485 490 495

Leu Leu His Ala Ala Gln Pro Ala Leu Thr Thr Thr Pro

500 505

<210> 64

<211> 509

<212> PRT

<213> Serratia marcescens PWN146

<400> 64

Met Ala Glu Ser Asn Ala Ala Ile Gln Ser Ala Ala Ile Ile Gly Ala

1 5 10 15

Gly Thr Met Gly Arg Gly Ile Ala Tyr Leu Phe Ala Gln Lys Gly Ile

20 25 30

Arg Thr Val Leu Tyr Asn Arg Asn Gly Asn Thr Leu Asn Gln Ala Arg

35 40 45

Asp Tyr Ile Glu Leu Asp Leu Asn Lys Lys Val Glu Gln Gly Lys Ile

50 55 60

Ala Leu Gln Asp Lys Gly Ala Val Leu Ala Asn Leu Val Phe Thr Ser

65 70 75 80

Val Phe Glu Thr Ile Ala Asp Ser Glu Leu Val Ile Glu Thr Ile Ala

85 90 95

Glu Gln Glu Gln Thr Lys Leu Glu Val Leu Ala Thr Ile Ala Ala Ala

100 105 110

Val Lys Pro Asp Thr Leu Ile Ala Thr Asn Thr Ser Ser Leu Ser Leu

115 120 125

Asn Lys Leu Ala Thr Ala Val Thr His Ser Glu Arg Phe Ile Gly Leu

130 135 140

His Phe Phe Asn Pro Ala Pro Leu Met Lys Leu Ile Glu Ile Ile Pro

145 150 155 160

Ala Tyr Phe Thr Ala His Ala Thr Thr Glu Arg Cys Arg Gln Leu Val

165 170 175

Ala Ala Leu Gly Lys Arg Asp Val Val Cys Gln Ala Thr Pro Gly Phe

180 185 190

Ile Val Asn Arg Met Ala Arg Pro Tyr Tyr Leu Glu Gly Phe Arg Leu

195 200 205

Leu Glu Glu His Val Ala Arg Ala Pro Gln Ile Asp Arg Ala Leu Lys

210 215 220

Ala Gly Gly His Phe Arg Met Gly Pro Leu Glu Leu Thr Asp Phe Ile

225 230 235 240

Gly Gln Asp Ile Asn Tyr Gln Val Ser Arg Gln Ile Trp Gln Asp Met

245 250 255

Gln Tyr Asp Ala Arg Tyr Thr Pro Gly His Leu Gln Arg Ser Leu Val

260 265 270

Asp Ala Gly Leu Leu Gly Lys Lys Asn Gly Arg Ser Tyr Phe Ala Val

275 280 285

Glu Glu Asn Ala Pro Pro Val Met Ala Ala Thr Asp Ala Asp Ile Glu

290 295 300

Thr Leu His Val Cys Gly Glu His Pro Phe Phe Thr Leu Leu Gln Gln

305 310 315 320

Arg Ala Ala Leu Gln Trp Pro Arg Leu Arg Val Glu Gln Arg Pro Ala

325 330 335

Leu Pro Gly Leu Gly Pro Ala Val Trp Ile Asn Asp Ala Phe Thr Val

340 345 350

Ser Ile Thr Asp Gly Arg Thr Ala Asn Gln Leu Ala Glu Gln Thr Ala

355 360 365

Ala Asp Ala Phe Val Val Asp Val Ala Leu Asn Tyr Ala Asp Thr Ala

370 375 380

Tyr Leu Val Ala Ala His Ser Arg His Ala Ser Ala Ala Asn Lys Ala

385 390 395 400

Leu Phe Leu Arg Leu Leu His Thr Ala Ile Pro Gln Val Glu Phe Ile

405 410 415

Lys Asp Ser Pro Ala Leu Ile Val Ala Arg Val Leu Ser Ser Leu Ile

420 425 430

Asn Glu Ser Val Ile Met Val Glu Ser Gly Val Cys Ser Arg Glu Asp

435 440 445

Ile Asp Val Ala Ala Val Ala Gly Val Asn Tyr Ala Asp Gly Ile Phe

450 455 460

Gly Trp Leu Asp Ser Leu Gly Glu Lys Asn Val Arg Ala Thr Leu Ser

465 470 475 480

Asn Leu Ala Gln Leu Leu His Ala Ala Arg Tyr Ala Pro His Tyr Thr

485 490 495

Leu Leu His Ala Ala Gln Pro Ala Leu Thr Thr Thr Pro

500 505

<210> 65

<211> 509

<212> PRT

<213> Serratia marcescens H1q

<400> 65

Met Ala Glu Arg Asn Ala Ala Ile Gln Ser Ala Ala Ile Ile Gly Ala

1 5 10 15

Gly Thr Met Gly Arg Gly Ile Ala Tyr Leu Phe Ala Gln Lys Gly Ile

20 25 30

Arg Thr Val Leu Tyr Asn Arg Asn Gly Asn Thr Leu Ile Gln Ala Arg

35 40 45

Glu Tyr Ile Val Gln Asp Leu Asp Lys Lys Val Glu Gln Gly Arg Leu

50 55 60

Ala Pro Gln Asp Lys Asp Ala Val Leu Ala Asn Leu Gln Phe Ser Ser

65 70 75 80

Val Phe Glu Ala Ile Val Asp Ser Asp Leu Val Leu Glu Thr Ile Ala

85 90 95

Glu Gln Glu Gln Ala Lys Leu Glu Val Leu Ala Ala Ile Ala Ala Ala

100 105 110

Val Lys Pro Asp Thr Leu Ile Ala Thr Asn Thr Ser Ser Leu Ser Leu

115 120 125

Asn Lys Leu Ala Thr Ala Val Thr His Ser Glu Arg Phe Ile Gly Leu

130 135 140

His Phe Phe Asn Pro Ala Pro Leu Met Lys Leu Ile Glu Ile Ile Pro

145 150 155 160

Ala Tyr Phe Thr Ala Gln Ala Thr Thr Glu Thr Cys Arg Gln Leu Val

165 170 175

Ala Ala Leu Gly Lys Arg Asp Val Val Cys Gln Ala Thr Pro Gly Phe

180 185 190

Ile Val Asn Arg Met Ala Arg Pro Tyr Tyr Leu Glu Gly Phe Arg Leu

195 200 205

Leu Glu Glu His Val Ala Arg Ala Pro Gln Ile Asp Arg Ala Leu Lys

210 215 220

Ala Gly Gly His Phe Arg Met Gly Pro Leu Glu Leu Thr Asp Phe Ile

225 230 235 240

Gly Gln Asp Ile Asn Tyr Gln Val Ser Arg Gln Ile Trp Gln Asp Met

245 250 255

Gln Tyr Asp Pro Arg Tyr Thr Pro Gly His Leu Gln Arg Ser Leu Val

260 265 270

Asp Ala Gly Leu Leu Gly Lys Lys Asn Gly Arg Ser Tyr Phe Ser Ala

275 280 285

Glu Glu Thr Ala Pro Pro Val Glu Ala Ala Val Glu Ala Asp Val Glu

290 295 300

Thr Leu Arg Ile Tyr Gly Glu His Pro Leu Phe Thr Leu Leu Gln Gln

305 310 315 320

Arg Ala Ala Leu Gln Trp Pro Gln Leu Arg Val Glu Gln Arg Pro Ala

325 330 335

Leu Ser Gly Leu Gly Ala Ala Ile Gln Val Asn Asp Ala Phe Thr Val

340 345 350

Ser Ile Thr Asp Gly Arg Thr Ala Asn Gln Leu Ala Glu Gln Thr Ala

355 360 365

Ala Asp Ala Phe Val Val Asp Val Ala Leu Asn Tyr Ala Asp Thr Ala

370 375 380

Tyr Leu Val Ala Ala His Ser Arg His Ala Ser Ala Ala Asn Lys Ala

385 390 395 400

Leu Phe Leu Arg Leu Leu His Thr Ala Leu Pro Gln Val Glu Phe Ile

405 410 415

Lys Asp Ser Pro Ala Leu Ile Val Ala Arg Val Leu Ser Ser Leu Ile

420 425 430

Asn Glu Ser Val Ile Met Val Glu Ser Gly Val Cys Ser Arg Glu Asp

435 440 445

Ile Asp Val Ala Ala Val Ala Gly Val Asn Tyr Ala Asp Gly Ile Phe

450 455 460

Gly Trp Leu Thr Arg Leu Gly Glu Lys Asn Val Arg Thr Thr Leu Ser

465 470 475 480

Asn Leu Ala Gln Leu Leu His Ala Ala Arg Tyr Ala Pro His Tyr Thr

485 490 495

Leu Leu His Ala Ala Gln Pro Ala Leu Thr Thr Thr Pro

500 505

<210> 66

<211> 509

<212> PRT

<213> Serratia marcescens UMH6

<400> 66

Met Ala Glu Ser Asn Ala Ala Ile Gln Ser Ala Ala Ile Ile Gly Ala

1 5 10 15

Gly Thr Met Gly Arg Gly Ile Ala Tyr Leu Phe Ala Gln Lys Ser Ile

20 25 30

Arg Thr Val Leu Tyr Asn Arg Asn Gly Asn Thr Leu Asn Gln Ala Arg

35 40 45

Asp Ala Ile Val Gln Asp Leu Asn Lys Lys Val Glu Gln Gly Lys Leu

50 55 60

Ala Leu Gln Asp Lys Asp Ala Val Leu Ala Asn Leu Thr Phe Ser Ala

65 70 75 80

Glu Phe Gly Ala Ile Ala Asp Ser Asp Leu Val Ile Glu Thr Ile Ala

85 90 95

Glu His Glu Gln Ala Lys Leu Glu Val Leu Ala Ala Ile Ala Ala Thr

100 105 110

Val Lys Pro Asp Thr Leu Ile Ala Thr Asn Thr Ser Ser Leu Ser Leu

115 120 125

Asn Lys Leu Ala Thr Ala Val Thr His Ser Glu Arg Phe Ile Gly Leu

130 135 140

His Phe Phe Asn Pro Ala Pro Leu Met Lys Leu Ile Glu Ile Ile Pro

145 150 155 160

Ala Tyr Phe Thr Ala Gln Val Thr Thr Glu Arg Cys Arg Gln Leu Val

165 170 175

Ala Ala Leu Gly Lys Arg Asp Val Val Cys Gln Ala Thr Pro Gly Phe

180 185 190

Ile Val Asn Arg Met Ala Arg Pro Tyr Tyr Leu Glu Gly Phe Arg Leu

195 200 205

Leu Glu Glu His Val Ala Arg Ala Pro Gln Ile Asp Arg Ala Leu Lys

210 215 220

Ala Gly Gly His Phe Arg Met Gly Pro Leu Glu Leu Thr Asp Phe Ile

225 230 235 240

Gly Gln Asp Ile Asn Tyr Gln Val Ser Arg Gln Ile Trp Gln Asp Met

245 250 255

Gln Tyr Asp Pro Arg Tyr Thr Pro Gly His Leu Gln Arg Ser Leu Val

260 265 270

Asp Ala Gly Leu Leu Gly Lys Lys Asn Gly Arg Ser Tyr Phe Ser Ala

275 280 285

Glu Glu Ser Pro Pro Pro Leu Ala Ala Ala Val Asp Ala Glu Val Glu

290 295 300

Thr Leu Arg Ile Tyr Gly Glu His Pro Leu Phe Thr Leu Leu Gln Gln

305 310 315 320

Arg Ala Ala Leu Gln Trp Pro Arg Leu Arg Val Glu Gln Arg Pro Thr

325 330 335

Leu Pro Gly Leu Gly Ala Ala Ile Gln Val Asn Asp Ala Phe Thr Val

340 345 350

Ser Val Thr Asp Gly Arg Thr Ala Asn Gln Leu Ala Glu Gln Thr Ala

355 360 365

Ala Asp Ala Phe Val Val Asp Val Ala Leu Asn Tyr Gly Asp Thr Ala

370 375 380

Tyr Leu Val Ala Ala His Ser Arg His Ala Ser Ala Ala Asn Lys Ala

385 390 395 400

Leu Phe Leu Arg Leu Leu His Thr Ala Ile Pro Gln Val Glu Phe Ile

405 410 415

Lys Asp Ser Pro Ala Leu Ile Val Ala Arg Val Leu Ser Ser Leu Ile

420 425 430

Asn Glu Ser Val Ile Met Val Glu Ser Gly Val Cys Ser Arg Glu Asp

435 440 445

Ile Asp Val Ala Ala Val Ala Gly Val Asn Tyr Ala Asp Gly Ile Phe

450 455 460

Gly Trp Leu Thr Arg Leu Gly Glu Glu Asn Val Arg Thr Thr Leu Ser

465 470 475 480

Asn Leu Ala Gln Leu Leu His Ala Ala Arg Tyr Ala Pro His Tyr Thr

485 490 495

Leu Leu His Ala Ala Gln Pro Ala Leu Thr Thr Thr Pro

500 505

<210> 67

<211> 509

<212> PRT

<213> Serratia nematodiphila WCU338

<400> 67

Met Ala Glu Ser Asn Ala Ala Ile Gln Ser Ala Ala Ile Ile Gly Ala

1 5 10 15

Gly Thr Met Gly Arg Gly Ile Ala Tyr Leu Phe Ala Gln Lys Ser Ile

20 25 30

Arg Thr Val Leu Tyr Asn Arg Asn Gly Asn Thr Leu Asn Gln Ala Arg

35 40 45

Asp Ala Ile Val Gln Asp Leu Asn Lys Lys Val Glu Gln Gly Lys Leu

50 55 60

Ala Leu Gln Asp Lys Asp Ala Val Leu Ala Asn Leu Thr Phe Ser Ala

65 70 75 80

Glu Phe Gly Ala Ile Ala Asp Ser Asp Leu Val Ile Glu Thr Ile Ala

85 90 95

Glu His Glu Gln Ala Lys Leu Glu Val Leu Ala Ala Ile Ala Ala Thr

100 105 110

Val Lys Pro Asp Thr Leu Ile Ala Thr Asn Thr Ser Ser Leu Ser Leu

115 120 125

Asn Lys Leu Ala Thr Ala Val Thr His Ser Glu Arg Phe Ile Gly Leu

130 135 140

His Phe Phe Asn Pro Ala Pro Leu Met Lys Leu Ile Glu Ile Ile Pro

145 150 155 160

Ala Tyr Phe Thr Ala Gln Ile Thr Thr Glu Arg Cys Arg Gln Leu Val

165 170 175

Ala Ala Leu Gly Lys Arg Asp Val Val Cys Gln Ala Thr Pro Gly Phe

180 185 190

Ile Val Asn Arg Met Ala Arg Pro Tyr Tyr Leu Glu Gly Phe Arg Leu

195 200 205

Leu Glu Glu His Val Ala Arg Ala Pro Gln Ile Asp Arg Ala Leu Lys

210 215 220

Ala Gly Gly His Phe Arg Met Gly Pro Leu Glu Leu Thr Asp Phe Ile

225 230 235 240

Gly Gln Asp Ile Asn Tyr Gln Val Ser Arg Gln Ile Trp Gln Asp Met

245 250 255

Gln Tyr Asp Pro Arg Tyr Thr Pro Gly His Leu Gln Arg Ser Leu Val

260 265 270

Asp Ala Gly Leu Leu Gly Lys Lys Asn Gly Arg Ser Tyr Phe Ser Ala

275 280 285

Glu Glu Ser Pro Pro Pro Leu Ala Ala Ala Val Asp Ala Glu Val Glu

290 295 300

Thr Leu Arg Ile Tyr Gly Glu His Pro Leu Phe Thr Leu Leu Gln Gln

305 310 315 320

Arg Ala Ala Leu Gln Trp Pro Arg Leu Arg Val Glu Gln Arg Pro Thr

325 330 335

Leu Pro Gly Leu Gly Ala Ala Ile Gln Val Asn Asp Ala Phe Thr Val

340 345 350

Ser Val Thr Asp Gly Arg Thr Ala Asn Gln Leu Ala Glu Gln Thr Ala

355 360 365

Ala Asp Ala Phe Val Val Asp Val Ala Leu Asn Tyr Gly Asp Thr Ala

370 375 380

Tyr Leu Val Ala Ala His Ser Arg His Ala Ser Ala Ala Asn Lys Ala

385 390 395 400

Leu Phe Leu Arg Leu Leu His Thr Ala Ile Pro Gln Val Glu Phe Ile

405 410 415

Lys Asp Ser Pro Ala Leu Ile Val Ala Arg Val Leu Ser Ser Leu Ile

420 425 430

Asn Glu Ser Val Ile Met Val Glu Ser Gly Val Cys Ser Arg Glu Asp

435 440 445

Ile Asp Val Ala Ala Val Ala Gly Val Asn Tyr Ala Asp Gly Ile Phe

450 455 460

Gly Trp Leu Thr Arg Leu Gly Glu Glu Asn Val Arg Thr Thr Leu Ser

465 470 475 480

Asn Leu Ala Gln Leu Leu His Ala Ala Arg Tyr Ala Pro His Tyr Thr

485 490 495

Leu Leu His Ala Ala Gln Pro Ala Leu Thr Thr Thr Pro

500 505

<210> 68

<211> 509

<212> PRT

<213> Serratia sp. OLEL1

<400> 68

Met Ala Glu Ser Asn Ala Ala Ile Gln Ser Ala Ala Ile Ile Gly Ala

1 5 10 15

Gly Thr Met Gly Arg Gly Ile Ala Tyr Leu Leu Ala Gln Lys Ser Ile

20 25 30

Arg Thr Val Leu Tyr Asn Arg Asn Gly Asn Thr Leu Asn Gln Ala Arg

35 40 45

Asp Ala Ile Val Gln Asp Leu Asn Lys Lys Val Glu Gln Gly Lys Leu

50 55 60

Ala Leu Gln Asp Lys Asp Ala Val Leu Ala Asn Leu Thr Phe Ser Ala

65 70 75 80

Glu Phe Gly Ala Ile Ala Asp Ser Asp Leu Val Ile Glu Thr Ile Ala

85 90 95

Glu His Glu Gln Ala Lys Leu Glu Val Leu Ala Ala Ile Ala Ala Thr

100 105 110

Val Lys Pro Asp Thr Leu Ile Ala Thr Asn Thr Ser Ser Leu Ser Leu

115 120 125

Asn Lys Leu Ala Thr Ala Val Thr His Ser Glu Arg Phe Ile Gly Leu

130 135 140

His Phe Phe Asn Pro Ala Pro Leu Met Lys Leu Ile Glu Ile Ile Pro

145 150 155 160

Ala Tyr Phe Thr Ala Gln Val Thr Thr Glu Arg Cys Arg Gln Leu Val

165 170 175

Ala Ala Leu Gly Lys Arg Asp Val Val Cys Gln Ala Thr Pro Gly Phe

180 185 190

Ile Val Asn Arg Met Ala Arg Pro Tyr Tyr Leu Glu Gly Phe Arg Leu

195 200 205

Leu Glu Glu His Val Ala Arg Ala Pro Gln Ile Asp Arg Ala Leu Lys

210 215 220

Ala Gly Gly His Phe Arg Met Gly Pro Leu Glu Leu Thr Asp Phe Ile

225 230 235 240

Gly Gln Asp Ile Asn Tyr Gln Val Ser Arg Gln Ile Trp Gln Asp Met

245 250 255

Gln Tyr Asp Pro Arg Tyr Thr Pro Gly His Leu Gln Arg Ser Leu Val

260 265 270

Asp Ala Gly Leu Leu Gly Lys Lys Asn Gly Arg Ser Tyr Phe Ser Ala

275 280 285

Glu Glu Ser Pro Pro Pro Leu Ala Ala Ala Val Asp Ala Glu Val Glu

290 295 300

Thr Leu Arg Ile Tyr Gly Glu His Pro Leu Phe Thr Leu Leu Gln Gln

305 310 315 320

Arg Ala Ala Leu Gln Trp Pro Arg Leu Arg Val Glu Gln Arg Pro Thr

325 330 335

Leu Pro Gly Leu Gly Ala Ala Ile Gln Val Asn Asp Ala Phe Thr Val

340 345 350

Ser Val Thr Asp Gly Arg Thr Ala Asn Gln Leu Ala Glu Gln Thr Ala

355 360 365

Ala Asp Ala Phe Val Val Asp Val Ala Leu Asn Tyr Gly Asp Thr Ala

370 375 380

Tyr Leu Val Ala Ala His Ser Arg His Ala Ser Ala Ala Asn Lys Ala

385 390 395 400

Leu Phe Leu Arg Leu Leu His Thr Ala Ile Pro Gln Val Glu Phe Ile

405 410 415

Lys Asp Ser Pro Ala Leu Ile Val Ala Arg Val Leu Ser Ser Leu Ile

420 425 430

Asn Glu Ser Val Ile Met Val Glu Ser Gly Val Cys Ser Arg Glu Asp

435 440 445

Ile Asp Val Ala Ala Val Ala Gly Val Asn Tyr Ala Asp Gly Ile Phe

450 455 460

Gly Trp Leu Thr Arg Leu Gly Glu Glu Asn Val Arg Thr Thr Leu Ser

465 470 475 480

Asn Leu Ala Gln Leu Leu His Ala Ala Arg Tyr Ala Pro His Tyr Thr

485 490 495

Leu Leu His Ala Ala Gln Pro Ala Leu Thr Thr Thr Pro

500 505

<210> 69

<211> 509

<212> PRT

<213> Serratia marcescens 7209

<400> 69

Met Ala Glu Ser Asn Ala Ala Ile Gln Ser Ala Ala Ile Ile Gly Ala

1 5 10 15

Gly Thr Met Gly Arg Gly Ile Ala Tyr Leu Phe Ala Gln Lys Ser Ile

20 25 30

Arg Thr Val Leu Tyr Asn Arg Asn Gly Asn Thr Leu Asn Gln Ala Arg

35 40 45

Asp Ala Ile Val Gln Asp Leu Asn Lys Lys Val Glu Gln Gly Lys Leu

50 55 60

Ala Leu Gln Asp Lys Asp Ala Val Leu Ala Asn Leu Thr Phe Ser Ala

65 70 75 80

Glu Phe Gly Val Ile Ala Asp Ser Asp Leu Val Ile Glu Thr Ile Ala

85 90 95

Glu His Glu Gln Ala Lys Leu Glu Val Leu Ala Ala Ile Ala Ala Thr

100 105 110

Val Lys Pro Asp Thr Leu Ile Ala Thr Asn Thr Ser Ser Leu Ser Leu

115 120 125

Asn Lys Leu Ala Thr Ala Val Thr His Ser Glu Arg Phe Ile Gly Leu

130 135 140

His Phe Phe Asn Pro Ala Pro Leu Met Lys Leu Ile Glu Ile Ile Pro

145 150 155 160

Ala Tyr Phe Thr Ala Gln Val Thr Thr Glu Arg Cys Arg Gln Leu Val

165 170 175

Ala Ala Leu Gly Lys Arg Asp Val Val Cys Gln Ala Thr Pro Gly Phe

180 185 190

Ile Val Asn Arg Met Ala Arg Pro Tyr Tyr Leu Glu Gly Phe Arg Leu

195 200 205

Leu Glu Glu His Val Ala Arg Ala Pro Gln Ile Asp Arg Ala Leu Lys

210 215 220

Ala Gly Gly His Phe Arg Met Gly Pro Leu Glu Leu Thr Asp Phe Ile

225 230 235 240

Gly Gln Asp Ile Asn Tyr Gln Val Ser Arg Gln Ile Trp Gln Asp Met

245 250 255

Gln Tyr Asp Pro Arg Tyr Thr Pro Gly His Leu Gln Arg Ser Leu Val

260 265 270

Asp Ala Gly Leu Leu Gly Lys Lys Asn Gly Arg Ser Tyr Phe Ser Ala

275 280 285

Glu Glu Ser Pro Pro Pro Leu Ala Ala Ala Val Asp Ala Glu Val Glu

290 295 300

Thr Leu Arg Ile Tyr Gly Glu His Pro Leu Phe Thr Leu Leu Gln Gln

305 310 315 320

Arg Ala Ala Leu Gln Trp Pro Arg Leu Arg Val Glu Gln Arg Pro Thr

325 330 335

Leu Pro Gly Leu Gly Ala Ala Ile Gln Val Asn Asp Ala Phe Thr Val

340 345 350

Ser Val Thr Asp Gly Arg Thr Ala Asn Gln Leu Ala Glu Gln Thr Ala

355 360 365

Ala Asp Ala Phe Val Val Asp Val Ala Leu Asn Tyr Gly Asp Thr Ala

370 375 380

Tyr Leu Val Ala Ala His Ser Arg His Ala Ser Ala Ala Asn Lys Ala

385 390 395 400

Leu Phe Leu Arg Leu Leu His Thr Ala Ile Pro Gln Val Glu Phe Ile

405 410 415

Lys Asp Ser Pro Ala Leu Ile Val Ala Arg Val Leu Ser Ser Leu Ile

420 425 430

Asn Glu Ser Val Ile Met Val Glu Ser Gly Val Cys Ser Arg Glu Asp

435 440 445

Ile Asp Val Ala Ala Val Ala Gly Val Asn Tyr Ala Asp Gly Ile Phe

450 455 460

Gly Trp Leu Thr Arg Leu Gly Glu Glu Asn Val Arg Thr Thr Leu Ser

465 470 475 480

Asn Leu Ala Gln Leu Leu His Ala Ala Arg Tyr Ala Pro His Tyr Thr

485 490 495

Leu Leu His Ala Ala Gln Pro Ala Leu Thr Thr Thr Pro

500 505

<210> 70

<211> 509

<212> PRT

<213> Serratia marcescens sicaria (Ss1)

<400> 70

Met Ala Glu Ser Asn Ala Ala Ile Gln Ser Ala Ala Ile Ile Gly Ala

1 5 10 15

Gly Thr Met Gly Arg Gly Ile Ala Tyr Leu Phe Ala Gln Lys Ser Ile

20 25 30

Arg Thr Val Leu Tyr Asn Arg Asn Gly Asn Thr Leu Asn Gln Ala Arg

35 40 45

Asp Ala Ile Val Gln Asp Leu Asn Lys Lys Val Glu Gln Gly Lys Leu

50 55 60

Ala Leu Gln Asp Lys Asp Ala Val Leu Ala Asn Leu Thr Phe Ser Ala

65 70 75 80

Glu Phe Gly Ala Ile Ala Asp Ser Asp Leu Val Ile Glu Thr Ile Ala

85 90 95

Glu His Glu Gln Ala Lys Leu Glu Val Leu Ala Ala Ile Ala Ala Thr

100 105 110

Val Lys Pro Asp Thr Leu Ile Ala Thr Asn Thr Ser Ser Leu Ser Leu

115 120 125

Asn Lys Leu Ala Thr Ala Val Thr His Ser Glu Arg Phe Ile Gly Leu

130 135 140

His Phe Phe Asn Pro Ala Pro Leu Met Lys Leu Ile Glu Ile Ile Pro

145 150 155 160

Ala Tyr Phe Thr Ala Gln Val Thr Thr Glu Arg Cys Arg Gln Leu Val

165 170 175

Ala Ala Leu Gly Lys Arg Asp Val Val Cys Gln Ala Thr Pro Gly Phe

180 185 190

Ile Val Asn Arg Met Ala Arg Pro Tyr Tyr Leu Glu Gly Phe Arg Leu

195 200 205

Leu Glu Glu His Val Ala Arg Ala Pro Gln Ile Asp Arg Ala Leu Lys

210 215 220

Ala Gly Gly His Phe Arg Met Gly Pro Leu Glu Leu Thr Asp Phe Ile

225 230 235 240

Gly Gln Asp Ile Asn Tyr Gln Val Ser Arg Gln Ile Trp Gln Asp Met

245 250 255

Gln Tyr Asp Pro Arg Tyr Thr Pro Gly His Leu Gln Arg Ser Leu Val

260 265 270

Asp Ala Gly Leu Leu Gly Lys Lys Asn Gly Arg Ser Tyr Phe Ser Thr

275 280 285

Glu Glu Ser Pro Pro Pro Leu Ala Ala Ala Val Asp Ala Glu Val Glu

290 295 300

Thr Leu Arg Ile Tyr Gly Glu His Pro Leu Phe Thr Leu Leu Gln Gln

305 310 315 320

Arg Ala Ala Leu Gln Trp Pro Arg Leu Arg Val Glu Gln Arg Pro Thr

325 330 335

Leu Pro Gly Leu Gly Ala Ala Ile Gln Val Asn Asp Ala Phe Thr Val

340 345 350

Ser Val Thr Asp Gly Arg Thr Ala Asn Gln Leu Ala Glu Gln Thr Ala

355 360 365

Ala Asp Ala Phe Val Val Asp Val Ala Leu Asn Tyr Gly Asp Thr Ala

370 375 380

Tyr Leu Val Ala Ala His Ser Arg His Ala Ser Ala Ala Asn Lys Ala

385 390 395 400

Leu Phe Leu Arg Leu Leu His Thr Ala Ile Pro Gln Val Glu Phe Ile

405 410 415

Lys Asp Ser Pro Ala Leu Ile Val Ala Arg Val Leu Ser Ser Leu Ile

420 425 430

Asn Glu Ser Val Ile Met Val Glu Ser Gly Val Cys Ser Arg Glu Asp

435 440 445

Ile Asp Val Ala Ala Val Ala Gly Val Asn Tyr Ala Asp Gly Ile Phe

450 455 460

Gly Trp Leu Thr Arg Leu Gly Glu Glu Asn Val Arg Thr Thr Leu Ser

465 470 475 480

Asn Leu Ala Gln Leu Leu His Ala Ala Arg Tyr Ala Pro His Tyr Thr

485 490 495

Leu Leu His Ala Ala Gln Pro Ala Leu Thr Thr Thr Pro

500 505

<210> 71

<211> 509

<212> PRT

<213> Serratia sp. OLFL2

<400> 71

Met Ala Glu Ser Asn Ala Ala Ile Gln Ser Ala Ala Ile Ile Gly Ala

1 5 10 15

Gly Thr Met Gly Arg Gly Ile Ala Tyr Leu Leu Ala Gln Lys Ser Ile

20 25 30

Arg Thr Val Leu Tyr Asn Arg Asn Gly Asn Thr Leu Asn Gln Ala Arg

35 40 45

Asp Ala Ile Val Gln Asp Leu Asn Lys Lys Val Glu Gln Gly Lys Leu

50 55 60

Ala Leu Gln Asp Lys Asp Ala Val Leu Ala Asn Leu Thr Phe Ser Ala

65 70 75 80

Glu Phe Gly Ala Ile Ala Asp Ser Asp Leu Val Ile Glu Thr Ile Ala

85 90 95

Glu His Glu Gln Ala Lys Leu Asp Val Leu Ala Ala Ile Ala Ala Thr

100 105 110

Val Lys Pro Asp Thr Leu Ile Ala Thr Asn Thr Ser Ser Leu Ser Leu

115 120 125

Asn Lys Leu Ala Thr Ala Val Thr His Ser Glu Arg Phe Ile Gly Leu

130 135 140

His Phe Phe Asn Pro Ala Pro Leu Met Lys Leu Ile Glu Ile Ile Pro

145 150 155 160

Ala Tyr Phe Thr Ala Gln Val Thr Thr Glu Arg Cys Arg Gln Leu Val

165 170 175

Ala Ala Leu Gly Lys Arg Asp Val Val Cys Gln Ala Thr Pro Gly Phe

180 185 190

Ile Val Asn Arg Met Ala Arg Pro Tyr Tyr Leu Glu Gly Phe Arg Leu

195 200 205

Leu Glu Glu His Val Ala Arg Ala Pro Gln Ile Asp Arg Ala Leu Lys

210 215 220

Ala Gly Gly His Phe Arg Met Gly Pro Leu Glu Leu Thr Asp Phe Ile

225 230 235 240

Gly Gln Asp Ile Asn Tyr Gln Val Ser Arg Gln Ile Trp Gln Asp Met

245 250 255

Gln Tyr Asp Pro Arg Tyr Thr Pro Gly His Leu Gln Arg Ser Leu Val

260 265 270

Asp Ala Gly Leu Leu Gly Lys Lys Asn Gly Arg Ser Tyr Phe Ser Ala

275 280 285

Glu Glu Ser Pro Pro Pro Leu Ala Ala Ala Val Asp Ala Glu Val Glu

290 295 300

Thr Leu Arg Ile Tyr Gly Glu His Pro Leu Phe Thr Leu Leu Gln Gln

305 310 315 320

Arg Ala Ala Leu Gln Trp Pro Arg Leu Arg Val Glu Gln Arg Pro Thr

325 330 335

Leu Pro Gly Leu Gly Ala Ala Ile Gln Val Asn Asp Ala Phe Thr Val

340 345 350

Ser Val Thr Asp Gly Arg Thr Ala Asn Gln Leu Ala Glu Gln Thr Ala

355 360 365

Ala Asp Ala Phe Val Val Asp Val Ala Leu Asn Tyr Gly Asp Thr Ala

370 375 380

Tyr Leu Val Ala Ala His Ser Arg His Ala Ser Ala Ala Asn Lys Ala

385 390 395 400

Leu Phe Leu Arg Leu Leu His Thr Ala Ile Pro Gln Val Glu Phe Ile

405 410 415

Lys Asp Ser Pro Ala Leu Ile Val Ala Arg Val Leu Ser Ser Leu Ile

420 425 430

Asn Glu Ser Val Ile Met Val Glu Ser Gly Val Cys Ser Arg Glu Asp

435 440 445

Ile Asp Val Ala Ala Val Ala Gly Val Asn Tyr Ala Asp Gly Ile Phe

450 455 460

Gly Trp Leu Thr Arg Leu Gly Glu Glu Asn Val Arg Thr Thr Leu Ser

465 470 475 480

Asn Leu Ala Gln Leu Leu His Ala Ala Arg Tyr Ala Pro His Tyr Thr

485 490 495

Leu Leu His Ala Ala Gln Pro Ala Leu Thr Thr Thr Pro

500 505

<210> 72

<211> 509

<212> PRT

<213> Serratia marcescens BIDMC 81

<400> 72

Met Ala Glu Ser Asn Ala Ala Ile Gln Ser Ala Ala Ile Ile Gly Ala

1 5 10 15

Gly Thr Met Gly Arg Gly Ile Ala Tyr Leu Leu Ala Gln Lys Ser Ile

20 25 30

Arg Thr Val Leu Tyr Asn Arg Asn Gly Asn Thr Leu Asn Gln Ala Arg

35 40 45

Asp Ala Ile Val Gln Asp Leu Asn Lys Lys Val Glu Gln Gly Lys Leu

50 55 60

Ala Leu Gln Asp Lys Asp Ala Val Leu Ala Asn Leu Thr Phe Ser Ala

65 70 75 80

Glu Phe Gly Ala Ile Ala Asp Ser Asp Leu Val Ile Glu Thr Ile Ala

85 90 95

Glu His Glu Gln Ala Lys Leu Glu Val Leu Ala Ala Ile Ala Ala Thr

100 105 110

Val Lys Pro Asp Thr Leu Ile Ala Thr Asn Thr Ser Ser Leu Ser Leu

115 120 125

Asn Lys Leu Ala Thr Ala Val Thr His Ser Glu Arg Phe Ile Gly Leu

130 135 140

His Phe Phe Asn Pro Ala Pro Leu Met Lys Leu Ile Glu Ile Ile Pro

145 150 155 160

Ala Tyr Phe Thr Ala Gln Val Thr Thr Glu Arg Cys Arg Gln Leu Val

165 170 175

Ala Ala Leu Gly Lys Arg Asp Val Val Cys Gln Ala Thr Pro Gly Phe

180 185 190

Ile Val Asn Arg Met Ala Arg Pro Tyr Tyr Leu Glu Gly Phe Arg Leu

195 200 205

Leu Glu Glu His Val Ala Arg Ala Pro Gln Ile Asp Arg Ala Leu Lys

210 215 220

Ala Gly Gly His Phe Arg Met Gly Pro Leu Glu Leu Thr Asp Phe Ile

225 230 235 240

Gly Gln Asp Ile Asn Tyr Gln Val Ser Arg Gln Ile Trp Gln Asp Met

245 250 255

Gln Tyr Asp Pro Arg Tyr Thr Pro Gly His Leu Gln Arg Ser Leu Val

260 265 270

Asp Ala Gly Leu Leu Gly Lys Lys Asn Gly Arg Ser Tyr Phe Ser Ala

275 280 285

Glu Glu Ser Pro Pro Pro Leu Ala Ala Ala Val Asp Ala Glu Val Glu

290 295 300

Thr Leu Arg Ile Tyr Gly Glu His Pro Leu Phe Thr Leu Leu Gln Gln

305 310 315 320

Arg Ala Ala Leu Gln Trp Pro Arg Leu Arg Val Glu Gln Arg Pro Thr

325 330 335

Leu Pro Asp Leu Gly Ala Ala Ile Gln Val Asn Asp Ala Phe Thr Val

340 345 350

Ser Val Thr Asp Gly Arg Thr Ala Asn Gln Leu Ala Glu Gln Thr Ala

355 360 365

Ala Asp Ala Phe Val Val Asp Val Ala Leu Asn Tyr Gly Asp Thr Ala

370 375 380

Tyr Leu Val Ala Ala His Ser Arg His Ala Ser Ala Ala Asn Lys Ala

385 390 395 400

Leu Phe Leu Arg Leu Leu His Thr Ala Ile Pro Gln Val Glu Phe Ile

405 410 415

Lys Asp Ser Pro Ala Leu Ile Val Ala Arg Val Leu Ser Ser Leu Ile

420 425 430

Asn Glu Ser Val Ile Met Val Glu Ser Gly Val Cys Ser Arg Glu Asp

435 440 445

Ile Asp Val Ala Ala Val Ala Gly Val Asn Tyr Ala Asp Gly Ile Phe

450 455 460

Gly Trp Leu Thr Arg Leu Gly Glu Glu Asn Val Arg Thr Thr Leu Ser

465 470 475 480

Asn Leu Ala Gln Leu Leu His Ala Ala Arg Tyr Ala Pro His Tyr Thr

485 490 495

Leu Leu His Ala Ala Gln Pro Ala Leu Thr Thr Thr Pro

500 505

<210> 73

<211> 509

<212> PRT

<213> Serratia marcescens BIDMC 50

<400> 73

Met Ala Glu Ser Asn Ala Ala Ile Gln Ser Ala Ala Ile Ile Gly Ala

1 5 10 15

Gly Thr Met Gly Arg Gly Ile Ala Tyr Leu Phe Ala Gln Lys Ser Ile

20 25 30

Arg Thr Val Leu Tyr Asn Arg Asn Gly Asn Thr Leu Asn Gln Ala Arg

35 40 45

Asp Ala Ile Val Gln Asp Leu Asn Lys Lys Val Glu Gln Gly Lys Leu

50 55 60

Ala Leu Gln Asp Lys Asp Val Val Leu Ala Asn Leu Thr Phe Ser Ala

65 70 75 80

Glu Phe Gly Ala Ile Ala Asp Ser Asp Leu Val Ile Glu Thr Ile Ala

85 90 95

Glu His Glu Gln Ala Lys Leu Glu Val Leu Ala Ala Ile Ala Ala Thr

100 105 110

Val Lys Pro Asp Thr Leu Ile Ala Thr Asn Thr Ser Ser Leu Ser Leu

115 120 125

Asn Lys Leu Ala Thr Ala Val Thr His Ser Glu Arg Phe Ile Gly Leu

130 135 140

His Phe Phe Asn Pro Ala Pro Leu Met Lys Leu Ile Glu Ile Ile Pro

145 150 155 160

Ala Tyr Phe Thr Ala Gln Val Thr Thr Glu Arg Cys Arg Gln Leu Val

165 170 175

Ala Ala Leu Gly Lys Arg Asp Val Val Cys Gln Ala Thr Pro Gly Phe

180 185 190

Ile Val Asn Arg Met Ala Arg Pro Tyr Tyr Leu Glu Gly Phe Arg Leu

195 200 205

Leu Glu Glu His Val Ala Arg Ala Pro Gln Ile Asp Arg Ala Leu Lys

210 215 220

Ala Gly Gly His Phe Arg Met Gly Pro Leu Glu Leu Thr Asp Phe Ile

225 230 235 240

Gly Gln Asp Ile Asn Tyr Gln Val Ser Arg Gln Ile Trp Gln Asp Met

245 250 255

Gln Tyr Asp Pro Arg Tyr Thr Pro Gly His Leu Gln Arg Ser Leu Val

260 265 270

Asp Ala Gly Leu Leu Gly Lys Lys Asn Gly Arg Ser Tyr Phe Ser Ala

275 280 285

Glu Glu Ser Pro Pro Pro Leu Ala Ala Ala Val Asp Ala Glu Val Glu

290 295 300

Thr Leu Arg Ile Tyr Gly Glu His Pro Leu Phe Thr Leu Leu Gln Gln

305 310 315 320

Arg Ala Ala Leu Gln Trp Pro Arg Leu Arg Val Glu Gln Arg Pro Thr

325 330 335

Leu Pro Asp Leu Gly Ala Ala Ile Gln Val Asn Asp Ala Phe Thr Val

340 345 350

Ser Val Thr Asp Gly Arg Thr Ala Asn Gln Leu Ala Glu Gln Thr Ala

355 360 365

Ala Asp Ala Phe Val Val Asp Val Ala Leu Asn Tyr Gly Asp Thr Ala

370 375 380

Tyr Leu Val Ala Ala His Ser Arg His Ala Ser Ala Ala Asn Lys Ala

385 390 395 400

Leu Phe Leu Arg Leu Leu His Thr Ala Ile Pro Gln Val Glu Phe Ile

405 410 415

Lys Asp Ser Pro Ala Leu Ile Val Ala Arg Val Leu Ser Ser Leu Ile

420 425 430

Asn Glu Ser Val Ile Met Val Glu Ser Gly Val Cys Ser Arg Glu Asp

435 440 445

Ile Asp Val Ala Ala Val Ala Gly Val Asn Tyr Ala Asp Gly Ile Phe

450 455 460

Gly Trp Leu Thr Arg Leu Gly Glu Glu Asn Val Arg Thr Thr Leu Ser

465 470 475 480

Asn Leu Ala Gln Leu Leu His Ala Ala Arg Tyr Ala Pro His Tyr Thr

485 490 495

Leu Leu His Ala Ala Gln Pro Ala Leu Thr Thr Thr Pro

500 505

<210> 74

<211> 509

<212> PRT

<213> Serratia marcescens UMH7

<400> 74

Met Ala Glu Ser Asn Ala Ala Ile Gln Ser Ala Ala Ile Ile Gly Ala

1 5 10 15

Gly Thr Met Gly Arg Gly Ile Ala Tyr Leu Leu Ala Gln Lys Ser Ile

20 25 30

Arg Thr Val Leu Tyr Asn Arg Asn Gly Asn Thr Leu Asn Gln Ala Arg

35 40 45

Asp Ala Ile Val Gln Asp Leu Asn Lys Lys Val Glu Gln Gly Lys Leu

50 55 60

Ala Leu Gln Asp Lys Asp Thr Val Leu Ala Asn Leu Thr Phe Ser Ala

65 70 75 80

Glu Phe Gly Ala Ile Ala Asp Ser Asp Leu Val Ile Glu Thr Ile Ala

85 90 95

Glu His Glu Gln Ala Lys Leu Glu Val Leu Ala Ala Ile Ala Ala Thr

100 105 110

Val Lys Pro Asp Thr Leu Ile Ala Thr Asn Thr Ser Ser Leu Ser Leu

115 120 125

Asn Lys Leu Ala Thr Ala Val Thr His Ser Glu Arg Phe Ile Gly Leu

130 135 140

His Phe Phe Asn Pro Ala Pro Leu Met Lys Leu Ile Glu Ile Ile Pro

145 150 155 160

Ala Tyr Phe Thr Ala Gln Val Thr Thr Glu Arg Cys Arg Gln Leu Val

165 170 175

Ala Ala Leu Gly Lys Arg Asp Val Val Cys Gln Ala Thr Pro Gly Phe

180 185 190

Ile Val Asn Arg Met Ala Arg Pro Tyr Tyr Leu Glu Gly Phe Arg Leu

195 200 205

Leu Glu Glu His Val Ala Arg Ala Pro Gln Ile Asp Arg Ala Leu Lys

210 215 220

Ala Gly Gly His Phe Arg Met Gly Pro Leu Glu Leu Thr Asp Phe Ile

225 230 235 240

Gly Gln Asp Ile Asn Tyr Gln Val Ser Arg Gln Ile Trp Gln Asp Met

245 250 255

Gln Tyr Asp Pro Arg Tyr Thr Pro Gly His Leu Gln Arg Ser Leu Val

260 265 270

Asp Ala Gly Leu Leu Gly Lys Lys Asn Gly Arg Ser Tyr Phe Ser Ala

275 280 285

Glu Glu Ser Pro Pro Pro Leu Ala Ala Ala Val Asp Ala Glu Val Glu

290 295 300

Thr Leu Arg Ile Tyr Gly Glu His Pro Leu Phe Thr Leu Leu Gln Gln

305 310 315 320

Arg Ala Thr Leu Gln Trp Pro Arg Leu Arg Val Glu Gln Arg Pro Thr

325 330 335

Leu Pro Gly Leu Gly Ala Ala Ile Gln Val Asn Asp Ala Phe Thr Val

340 345 350

Ser Val Thr Asp Gly Arg Thr Ala Asn Gln Leu Ala Glu Gln Thr Ala

355 360 365

Ala Asp Ala Phe Val Val Asp Val Ala Leu Asn Tyr Gly Asp Thr Ala

370 375 380

Tyr Leu Val Ala Ala His Ser Arg His Ala Ser Ala Ala Asn Lys Ala

385 390 395 400

Leu Phe Leu Arg Leu Leu His Thr Ala Ile Pro Gln Val Glu Phe Ile

405 410 415

Lys Asp Ser Pro Ala Leu Ile Val Ala Arg Val Leu Ser Ser Leu Ile

420 425 430

Asn Glu Ser Val Ile Met Val Glu Ser Gly Val Cys Ser Arg Glu Asp

435 440 445

Ile Asp Val Ala Ala Val Ala Gly Val Asn Tyr Ala Asp Gly Ile Phe

450 455 460

Gly Trp Leu Thr Arg Leu Gly Glu Glu Asn Val Arg Thr Thr Leu Ser

465 470 475 480

Asn Leu Ala Gln Leu Leu His Ala Ala Arg Tyr Ala Pro His Tyr Thr

485 490 495

Leu Leu His Ala Ala Gln Pro Ala Leu Thr Thr Thr Pro

500 505

<210> 75

<211> 509

<212> PRT

<213> Serratia marcescens RSC-14

<400> 75

Met Ala Glu Ser Asn Ala Ala Ile Gln Ser Ala Ala Ile Ile Gly Ala

1 5 10 15

Gly Thr Met Gly Arg Gly Ile Ala Tyr Leu Leu Ala Gln Lys Ser Ile

20 25 30

Arg Thr Val Leu Tyr Asn Arg Asn Gly Asn Thr Leu Asn Gln Ala Arg

35 40 45

Asp Ala Ile Val Gln Asp Leu Asn Lys Lys Val Glu Gln Gly Lys Leu

50 55 60

Ala Leu Gln Asp Lys Asp Ala Val Leu Ala Asn Leu Thr Phe Ser Thr

65 70 75 80

Glu Phe Gly Ala Ile Ala Asp Ser Asp Leu Val Ile Glu Thr Ile Ala

85 90 95

Glu His Glu Gln Ala Lys Leu Glu Val Leu Ala Ala Ile Ala Ala Thr

100 105 110

Val Lys Pro Asp Arg Leu Ile Ala Thr Asn Thr Ser Ser Leu Ser Leu

115 120 125

Asn Lys Leu Ala Thr Ala Val Thr His Ser Glu Arg Phe Ile Gly Leu

130 135 140

His Phe Phe Asn Pro Ala Pro Leu Met Lys Leu Ile Glu Ile Ile Pro

145 150 155 160

Ala Tyr Phe Thr Ala Gln Val Thr Thr Glu Arg Cys Arg Gln Leu Val

165 170 175

Ala Ala Leu Gly Lys Arg Asp Val Val Cys Gln Ala Thr Pro Gly Phe

180 185 190

Ile Val Asn Arg Met Ala Arg Pro Tyr Tyr Leu Glu Gly Phe Arg Leu

195 200 205

Leu Glu Glu His Val Ala Arg Ala Pro Gln Ile Asp Arg Ala Leu Lys

210 215 220

Ala Gly Gly His Phe Arg Met Gly Pro Leu Glu Leu Thr Asp Phe Ile

225 230 235 240

Gly Gln Asp Ile Asn Tyr Gln Val Ser Arg Gln Ile Trp Gln Asp Met

245 250 255

Gln Tyr Asp Pro Arg Tyr Thr Pro Gly His Leu Gln Arg Ser Leu Val

260 265 270

Asp Ala Gly Leu Leu Gly Lys Lys Asn Gly Arg Ser Tyr Phe Ser Ala

275 280 285

Glu Glu Ser Pro Pro Pro Leu Ala Ala Ala Val Asp Ala Glu Val Glu

290 295 300

Thr Leu Arg Ile Tyr Gly Glu His Pro Leu Phe Thr Leu Leu Gln Gln

305 310 315 320

Arg Ala Ala Leu Gln Trp Pro Arg Leu Arg Val Glu Gln Gln Pro Thr

325 330 335

Leu Pro Gly Leu Gly Ala Ala Ile Gln Val Asn Asp Ala Phe Thr Val

340 345 350

Ser Val Thr Asp Gly Arg Thr Ala Asn Gln Leu Ala Glu Gln Thr Ala

355 360 365

Ala Asp Ala Phe Val Val Asp Val Ala Leu Asn Tyr Gly Asp Thr Ala

370 375 380

Tyr Leu Val Ala Ala His Ser Arg His Ala Ser Ala Ala Asn Lys Ala

385 390 395 400

Leu Phe Leu Arg Leu Leu His Thr Ala Ile Pro Gln Val Glu Phe Ile

405 410 415

Lys Asp Ser Pro Ala Leu Ile Val Ala Arg Val Leu Ser Ser Leu Ile

420 425 430

Asn Glu Ser Val Ile Met Val Glu Ser Gly Val Cys Ser Arg Glu Asp

435 440 445

Ile Asp Val Ala Ala Val Ala Gly Val Asn Tyr Ala Asp Gly Ile Phe

450 455 460

Gly Trp Leu Thr Arg Leu Gly Glu Glu Asn Val Arg Thr Thr Leu Ser

465 470 475 480

Asn Leu Ala Gln Leu Leu His Ala Ala Arg Tyr Ala Pro His Tyr Thr

485 490 495

Leu Leu His Ala Ala Gln Pro Ala Leu Thr Thr Thr Pro

500 505

<210> 76

<211> 509

<212> PRT

<213> Serratia marcescens SM03

<400> 76

Met Ala Glu Arg Asn Ala Ala Ile Gln Ser Ala Ala Ile Ile Gly Ala

1 5 10 15

Gly Thr Met Gly Arg Gly Ile Ala Tyr Leu Phe Ala Gln Lys Gly Ile

20 25 30

Arg Thr Val Leu Tyr Asn Arg Asn Gly Asn Thr Leu Ile Gln Ala Arg

35 40 45

Glu Tyr Ile Val Gln Asp Leu Asp Lys Lys Val Glu Gln Gly Arg Leu

50 55 60

Ala Pro Gln Asp Lys Asp Ala Val Leu Ala Asn Leu Gln Phe Ser Ser

65 70 75 80

Val Phe Glu Ala Ile Val Asp Ser Asp Leu Val Leu Glu Thr Ile Ala

85 90 95

Glu Gln Glu Gln Ala Lys Leu Glu Val Leu Ala Ala Ile Ala Ala Ala

100 105 110

Val Lys Pro Asp Thr Leu Ile Ala Thr Asn Thr Ser Ser Leu Ser Leu

115 120 125

Asn Lys Leu Ala Thr Ala Val Thr His Ser Glu Arg Phe Ile Gly Leu

130 135 140

His Phe Phe Asn Pro Ala Pro Leu Met Lys Leu Ile Glu Ile Ile Pro

145 150 155 160

Ala Tyr Phe Thr Ala Gln Ala Thr Thr Glu Thr Cys Arg Gln Leu Val

165 170 175

Ala Ala Leu Gly Lys Arg Asp Val Val Cys Gln Ala Thr Pro Gly Phe

180 185 190

Ile Val Asn Arg Met Ala Arg Pro Tyr Tyr Leu Glu Gly Phe Arg Leu

195 200 205

Leu Glu Glu His Val Ala Arg Ala Pro Gln Ile Asp Arg Ala Leu Lys

210 215 220

Ala Gly Gly His Phe Arg Met Gly Pro Leu Glu Leu Thr Asp Phe Ile

225 230 235 240

Gly Gln Asp Ile Asn Tyr Gln Val Ser Arg Gln Ile Trp Gln Asp Met

245 250 255

Gln Tyr Asp Pro Arg Tyr Thr Pro Gly His Leu Gln Arg Ser Leu Val

260 265 270

Asp Ala Gly Leu Leu Gly Lys Lys Asn Gly Arg Ser Tyr Phe Ser Ala

275 280 285

Glu Glu Thr Ala Pro Pro Val Glu Ala Ala Ala Glu Ala Asp Val Glu

290 295 300

Thr Leu Arg Ile Tyr Gly Glu His Pro Leu Phe Thr Leu Leu Gln Gln

305 310 315 320

Arg Ala Ala Leu Gln Trp Pro Gln Leu Arg Val Glu Gln Arg Pro Ala

325 330 335

Leu Ser Gly Leu Gly Ala Ala Ile Gln Val Asn Asp Ala Phe Thr Val

340 345 350

Ser Val Thr Asp Gly Arg Thr Ala Asn Gln Leu Ala Glu Gln Thr Ala

355 360 365

Ala Asp Ala Phe Val Val Asp Val Ala Leu Asn Tyr Ala Asp Thr Ala

370 375 380

Tyr Leu Val Ala Ala His Ser Arg His Ala Ser Ala Ala Asn Lys Ala

385 390 395 400

Leu Phe Leu Arg Leu Leu His Thr Ala Leu Pro Gln Val Glu Phe Ile

405 410 415

Lys Asp Ser Pro Ala Leu Ile Val Ala Arg Val Leu Ser Ser Leu Ile

420 425 430

Asn Glu Ser Val Ile Met Val Glu Ser Gly Val Cys Ser Arg Glu Asp

435 440 445

Ile Asp Val Ala Ala Val Ala Gly Val Asn Tyr Ala Asp Gly Ile Phe

450 455 460

Gly Trp Leu Thr Arg Leu Gly Glu Lys Asn Val Arg Thr Thr Leu Ser

465 470 475 480

Asn Leu Ala Gln Leu Leu His Ala Ala Arg Tyr Ala Pro His Tyr Thr

485 490 495

Leu Leu His Ala Ala Gln Pro Ala Leu Thr Thr Thr Pro

500 505

<210> 77

<211> 509

<212> PRT

<213> Serratia marcescens 90-166

<400> 77

Met Ala Glu Ser Asn Ala Ala Ile Gln Ser Ala Ala Ile Ile Gly Ala

1 5 10 15

Gly Thr Met Gly Arg Gly Ile Ala Tyr Leu Phe Ala Gln Lys Gly Ile

20 25 30

Arg Thr Val Leu Tyr Asn Arg Asn Gly Asn Thr Leu Ile Gln Ala Arg

35 40 45

Glu Tyr Ile Val Gln Asp Leu Asp Lys Lys Ile Glu Gln Gly Arg Val

50 55 60

Ala Pro Gln Asp Lys Asp Ala Val Leu Ala Asn Leu Thr Phe Ser Ala

65 70 75 80

Glu Phe Gly Ala Ile Val Asp Ser Asp Leu Val Ile Glu Thr Ile Ala

85 90 95

Glu His Glu Gln Ala Lys Leu Glu Val Leu Ala Ala Ile Ala Ala Thr

100 105 110

Val Lys Pro Asp Thr Leu Ile Ala Thr Asn Thr Ser Ser Leu Ser Leu

115 120 125

Asn Lys Leu Ala Thr Ala Val Thr His Ser Glu Arg Phe Ile Gly Leu

130 135 140

His Phe Phe Asn Pro Ala Pro Leu Met Lys Leu Ile Glu Ile Ile Pro

145 150 155 160

Ala Tyr Phe Thr Ala Gln Val Thr Thr Glu Arg Cys Arg Gln Leu Val

165 170 175

Ala Ala Leu Gly Lys Arg Asp Val Val Cys Gln Ala Thr Pro Gly Phe

180 185 190

Ile Val Asn Arg Met Ala Arg Pro Tyr Tyr Leu Glu Gly Phe Arg Leu

195 200 205

Leu Glu Glu His Val Ala Arg Ala Pro Gln Ile Asp Arg Ala Leu Lys

210 215 220

Ala Gly Gly His Phe Arg Met Gly Pro Leu Glu Leu Thr Asp Phe Ile

225 230 235 240

Gly Gln Asp Ile Asn Tyr Gln Val Ser Arg Gln Ile Trp Gln Asp Met

245 250 255

Gln Tyr Asp Pro Arg Tyr Thr Pro Gly His Leu Gln Arg Ser Leu Val

260 265 270

Asp Ala Gly Leu Leu Gly Lys Lys Asn Gly Arg Ser Tyr Phe Ser Ala

275 280 285

Glu Glu Ser Pro Pro Pro Leu Ala Ala Ala Val Asp Ala Glu Ile Glu

290 295 300

Thr Leu Arg Ile Tyr Gly Glu His Pro Leu Phe Thr Leu Leu Gln Gln

305 310 315 320

Arg Ala Ala Leu Gln Trp Pro Arg Leu Arg Val Glu His Arg Pro Thr

325 330 335

Leu Pro Gly Leu Gly Ala Ala Ile Gln Val Asn Asp Ala Phe Thr Val

340 345 350

Ser Val Thr Asp Gly Arg Thr Ala Asn Gln Leu Ala Glu Gln Thr Ala

355 360 365

Ala Asp Ala Phe Val Val Asp Val Ala Leu Asn Tyr Gly Asp Thr Ala

370 375 380

Tyr Leu Ala Ala Ala His Ser Arg His Ala Ser Ala Ala Asn Lys Ala

385 390 395 400

Leu Phe Leu Arg Leu Leu His Thr Ala Ile Pro Gln Val Glu Phe Ile

405 410 415

Lys Asp Ser Pro Ala Leu Ile Val Ala Arg Val Leu Ser Ser Leu Ile

420 425 430

Asn Glu Ser Val Ile Met Val Glu Ser Gly Val Cys Ser Arg Glu Asp

435 440 445

Ile Asp Val Ala Ala Val Ala Gly Val Asn Tyr Ala Asp Gly Ile Phe

450 455 460

Val Trp Leu Thr Arg Leu Gly Glu Glu Asn Val Arg Thr Thr Leu Ser

465 470 475 480

Asn Leu Ala Gln Leu Leu His Ala Ala Arg Tyr Ala Pro His Tyr Thr

485 490 495

Leu Leu His Ala Ala Gln Pro Ala Leu Thr Ser Thr Pro

500 505

<210> 78

<211> 509

<212> PRT

<213> Serratia marcescens UMH2

<400> 78

Met Ala Glu Ser Asn Ala Ala Ile Gln Ser Ala Ala Ile Ile Gly Ala

1 5 10 15

Gly Thr Met Gly Arg Gly Ile Ala Tyr Leu Leu Ala Gln Lys Ser Ile

20 25 30

Arg Thr Val Leu Tyr Asn Arg Asn Gly Asn Thr Leu Asn Gln Ala Arg

35 40 45

Asp Ala Ile Val Gln Asp Leu Asn Lys Lys Val Glu Gln Gly Lys Leu

50 55 60

Ala Leu Gln Asp Lys Asp Ala Val Leu Ala Asn Leu Thr Phe Ser Thr

65 70 75 80

Glu Phe Gly Ala Ile Ala Asp Ser Asp Leu Val Ile Glu Thr Ile Ala

85 90 95

Glu His Glu Gln Ala Lys Leu Glu Val Leu Ala Ala Ile Ala Ala Thr

100 105 110

Val Lys Pro Asp Thr Leu Ile Ala Thr Asn Thr Ser Ser Leu Ser Leu

115 120 125

Asn Lys Leu Ala Thr Ala Val Thr His Ser Glu Arg Phe Ile Gly Leu

130 135 140

His Phe Phe Asn Pro Ala Pro Leu Met Lys Leu Ile Glu Ile Ile Pro

145 150 155 160

Ala Tyr Phe Thr Ala Gln Val Thr Thr Glu Arg Cys Arg Gln Leu Val

165 170 175

Ala Ala Leu Gly Lys Arg Asp Val Val Cys Gln Ala Thr Pro Gly Phe

180 185 190

Ile Val Asn Arg Met Ala Arg Pro Tyr Tyr Leu Glu Gly Phe Arg Leu

195 200 205

Leu Glu Glu His Val Ala Arg Ala Pro Gln Ile Asp Arg Ala Leu Lys

210 215 220

Ala Gly Gly His Phe Arg Met Gly Pro Leu Glu Leu Thr Asp Phe Ile

225 230 235 240

Gly Gln Asp Ile Asn Tyr Gln Val Ser Arg Gln Ile Trp Gln Asp Met

245 250 255

Gln Tyr Asp Pro Arg Tyr Thr Pro Gly His Leu Gln Arg Ser Leu Val

260 265 270

Asp Ala Gly Leu Leu Gly Lys Lys Asn Gly Arg Ser Tyr Phe Ser Ala

275 280 285

Glu Glu Ser Pro Pro Pro Leu Ala Ala Ala Val Asp Ala Glu Val Glu

290 295 300

Ala Leu Arg Ile Tyr Gly Glu His Pro Leu Phe Thr Leu Leu Gln Gln

305 310 315 320

Arg Ala Ala Leu Gln Trp Pro Arg Leu Arg Val Glu Gln Arg Pro Thr

325 330 335

Leu Pro Gly Leu Gly Ala Ala Ile Gln Val Asn Asp Ala Phe Thr Val

340 345 350

Ser Val Thr Asp Gly Arg Thr Ala Asn Gln Leu Ala Glu Gln Thr Ala

355 360 365

Ala Asp Ala Phe Val Val Asp Val Ala Leu Asn Tyr Gly Asp Thr Ala

370 375 380

Tyr Leu Val Ala Ala His Ser Arg His Ala Ser Ala Ala Asn Lys Ala

385 390 395 400

Leu Phe Leu Arg Leu Leu His Thr Ala Ile Pro Gln Val Glu Phe Ile

405 410 415

Lys Asp Ser Pro Ala Leu Ile Val Ala Arg Val Leu Ser Ser Leu Ile

420 425 430

Asn Glu Ser Val Ile Met Val Glu Ser Gly Val Cys Ser Arg Glu Asp

435 440 445

Ile Asp Val Ala Ala Val Ala Gly Val Asn Tyr Ala Asp Gly Ile Phe

450 455 460

Gly Trp Leu Thr His Leu Gly Glu Glu Asn Val Arg Thr Thr Leu Ser

465 470 475 480

Asn Leu Ala Gln Leu Leu His Ala Ala Arg Tyr Ala Pro His Tyr Thr

485 490 495

Leu Leu His Ala Ala Gln Pro Ala Leu Thr Thr Thr Pro

500 505

<210> 79

<211> 509

<212> PRT

<213> Serratia plymuthica AS9

<400> 79

Met Ala Glu Asn Asn Ser Ala Ile Arg Ser Ala Ala Val Ile Gly Ala

1 5 10 15

Gly Thr Met Gly Arg Gly Ile Ala Tyr Leu Leu Ala Leu Asn Gly Ile

20 25 30

Arg Thr Val Leu Tyr Asn Arg Asn Gly Asn Asn Leu Asn Gln Ala Arg

35 40 45

Asp Tyr Ile Val Ser Asp Leu Asp Arg Lys Met Asp Asn Gly Lys Ile

50 55 60

Thr Leu Gln Lys Lys Gly Gln Ile Leu Ala Asn Ile Ile Phe Ser Ala

65 70 75 80

Val Phe Asp Ala Ile Thr Asp Ser Asp Leu Val Ile Glu Thr Ile Ala

85 90 95

Glu Asp Glu Gln Thr Lys His Glu Ile Leu Ala Ala Ile Ala Ala Thr

100 105 110

Val Lys Pro Glu Ala Ile Ile Ala Thr Asn Thr Ser Ser Leu Ser Leu

115 120 125

Asn Lys Leu Ala Ala Gly Val Glu Asn Asn Pro Arg Phe Ile Gly Leu

130 135 140

His Phe Phe Asn Pro Ala Pro Leu Met Lys Leu Ile Glu Ile Ile Pro

145 150 155 160

Ser Tyr Phe Thr Ser His Thr Thr Ser Leu Arg Cys Gln Gln Leu Val

165 170 175

Ile Ala Leu Gly Lys Gln Phe Val Val Cys Lys Ala Thr Pro Gly Phe

180 185 190

Ile Val Asn Arg Met Ala Arg Pro Phe Tyr Leu Glu Gly Phe Arg Leu

195 200 205

Leu Glu Glu Asn Val Ala Gln Ala Pro Gln Ile Asp Arg Ala Leu Lys

210 215 220

Ala Gly Gly His Phe Arg Met Gly Pro Leu Glu Leu Thr Asp Phe Ile

225 230 235 240

Gly Gln Asp Ile Asn Tyr Gln Val Ser Lys Gln Ile Trp Gln Asp Met

245 250 255

Gln Phe Asp Ser Arg Tyr Thr Pro Gly His Leu Gln Arg Ser Leu Val

260 265 270

Asp Ala Gly Leu Leu Gly Arg Lys Asn Gly Arg Ser Phe Phe Ala Ser

275 280 285

Leu Pro Ala Thr Pro Pro Thr Pro Ala Thr Glu Ser Asp Thr Pro Thr

290 295 300

Ser Leu His Phe Tyr Gly Glu His Ala Leu Phe Asp His Leu Gln Gln

305 310 315 320

Arg Ala Leu Ala Thr Trp Pro Ala Leu Arg Val Gln Arg Leu Pro Glu

325 330 335

Arg Pro Glu Leu Gly Arg Leu Ile Leu Val Asn Asn Thr Leu Ala Ile

340 345 350

Lys Ile Thr Asp Gly Arg Thr Ala Asn Leu Leu Ala Gly Leu Thr Ala

355 360 365

Leu Asp Thr Phe Val Ile Asp Ala Ala Leu Asn Tyr Ala Asp Thr Ala

370 375 380

Tyr Leu Val Ala Ala His Asn Gln His Ala Thr Glu Ala Asn Lys Ala

385 390 395 400

Leu Phe Leu Ser Leu Leu Gln Thr Val Ile Ala Gln Val Glu Phe Ile

405 410 415

Lys Asp Ser Pro Ala Leu Ile Val Ala Arg Val Leu Ser Ser Leu Ile

420 425 430

Asn Glu Ser Val Ile Met Val Glu Ser Gly Val Cys Ser Arg Ala Asp

435 440 445

Ile Asp Ile Ala Ala Val Ala Gly Val Asn Tyr Ala Asp Gly Ile Phe

450 455 460

Ala Trp Leu Ala Gln Leu Gly Gln Lys Asn Val Lys Ser Thr Leu Asp

465 470 475 480

Asn Met Ala Gln Leu Leu His Ser Ala Arg Tyr Tyr Pro His Tyr Ser

485 490 495

Leu Leu Asn Ala Ala Arg Pro Glu Leu Ala Val Ala Pro

500 505

<210> 80

<211> 509

<212> PRT

<213> Serratia plymuthica tumat 205

<400> 80

Met Ala Glu Asn Asn Ser Ala Ile Arg Ser Ala Ala Val Ile Gly Ala

1 5 10 15

Gly Thr Met Gly Arg Gly Ile Ala Tyr Leu Leu Ala Leu Asn Gly Ile

20 25 30

Arg Thr Val Leu Tyr Asn Arg Asn Gly Asn Asn Leu Asn Gln Ala Arg

35 40 45

Asp Tyr Ile Val Ser Asp Leu Asp Arg Lys Ile Asp Asn Gly Lys Ile

50 55 60

Thr Leu Gln Lys Lys Gly Gln Ile Leu Ala Asn Ile Ile Phe Ser Ala

65 70 75 80

Val Phe Asp Ala Ile Thr Asp Ser Asp Leu Val Ile Glu Thr Ile Ala

85 90 95

Glu Asp Glu Gln Thr Lys His Glu Ile Leu Ala Ala Ile Ala Ala Thr

100 105 110

Val Lys Pro Glu Ala Ile Ile Ala Thr Asn Thr Ser Ser Leu Ser Leu

115 120 125

Asn Lys Leu Ala Ala Gly Val Glu Asn Asn Pro Arg Phe Ile Gly Leu

130 135 140

His Phe Phe Asn Pro Ala Pro Leu Met Lys Leu Ile Glu Ile Ile Pro

145 150 155 160

Ser Tyr Phe Thr Ser His Ala Thr Ser Leu Arg Cys Gln Gln Leu Val

165 170 175

Ile Ala Leu Gly Lys Gln Phe Val Val Cys Lys Ala Thr Pro Gly Phe

180 185 190

Ile Val Asn Arg Met Ala Arg Pro Phe Tyr Leu Glu Gly Phe Arg Leu

195 200 205

Leu Glu Glu Asn Val Ala Leu Ala Pro Gln Ile Asp Arg Ala Leu Lys

210 215 220

Ala Gly Gly His Phe Arg Met Gly Pro Leu Glu Leu Thr Asp Phe Ile

225 230 235 240

Gly Gln Asp Ile Asn Tyr Gln Val Ser Lys Gln Ile Trp Gln Asp Met

245 250 255

Gln Phe Asp Pro Arg Tyr Thr Pro Gly His Leu Gln Arg Ser Leu Val

260 265 270

Asp Ala Gly Leu Leu Gly Arg Lys Asn Gly Arg Ser Phe Phe Ala Ser

275 280 285

Gln Pro Val Thr Pro Pro Thr Pro Thr Thr Glu Ser Asp Thr Pro Thr

290 295 300

Ser Leu His Phe Tyr Gly Glu His Ala Leu Phe Asp His Leu Gln Gln

305 310 315 320

Arg Ala Leu Ala Thr Trp Pro Ala Leu Arg Val Gln Arg Leu Pro Glu

325 330 335

Arg Pro Glu Leu Gly Arg Phe Ile Leu Val Asn Asn Ala Met Ala Ile

340 345 350

Lys Ile Thr Asp Gly Arg Thr Ala Asn Leu Leu Ala Gly Leu Thr Ala

355 360 365

Leu Asp Thr Phe Val Ile Asp Ala Ala Leu Asn Tyr Ala Asp Thr Ala

370 375 380

Tyr Leu Val Ala Ala His Asn Gln His Ala Thr Glu Thr Asn Lys Ala

385 390 395 400

Leu Phe Leu Thr Leu Leu Gln Thr Val Ile Ala Gln Val Glu Phe Ile

405 410 415

Lys Asp Ser Pro Ala Leu Ile Val Ala Arg Val Leu Ser Ser Leu Ile

420 425 430

Asn Glu Ser Val Ile Met Val Glu Ser Gly Val Cys Ser Arg Ala Asp

435 440 445

Ile Asp Ile Ala Ala Val Ala Gly Val Asn Tyr Ala Asp Gly Ile Phe

450 455 460

Ala Trp Leu Ala Gln Leu Gly Gln Lys Asn Val Lys Ser Thr Leu Asp

465 470 475 480

Asn Met Ala Gln Leu Leu His Ser Ala Arg Tyr Tyr Pro His Tyr Ser

485 490 495

Leu Leu Asn Ala Ala Arg Pro Glu Leu Ala Val Ala Pro

500 505

<210> 81

<211> 509

<212> PRT

<213> Serratia plymuthica A30

<400> 81

Met Ala Glu Asn Asn Ser Ala Ile Arg Ser Ala Ala Val Ile Gly Ala

1 5 10 15

Gly Thr Met Gly Arg Gly Ile Ala Tyr Leu Leu Ala Leu Asn Gly Ile

20 25 30

Arg Thr Val Leu Tyr Asn Arg Asn Gly Asn Asn Leu Asn Gln Ala Arg

35 40 45

Asp Tyr Ile Val Ser Asp Leu Asp Arg Lys Ile Asp Asn Gly Lys Ile

50 55 60

Thr Leu Gln Lys Lys Gly Gln Ile Leu Ala Asn Ile Ile Phe Ser Ala

65 70 75 80

Val Phe Asp Ala Ile Thr Asp Ser Asp Leu Val Ile Glu Thr Ile Ala

85 90 95

Glu Asp Glu Gln Thr Lys His Glu Ile Leu Ala Ala Ile Ala Ala Thr

100 105 110

Val Lys Pro Glu Ala Ile Ile Ala Thr Asn Thr Ser Ser Leu Ser Leu

115 120 125

Asn Lys Leu Ala Ala Gly Val Glu Asn Asn Pro Arg Phe Ile Gly Leu

130 135 140

His Phe Phe Asn Pro Ala Pro Leu Met Lys Leu Ile Glu Ile Ile Pro

145 150 155 160

Ser Tyr Phe Thr Ser His Ala Thr Ser Leu Arg Cys Gln Lys Leu Val

165 170 175

Ile Ala Leu Gly Lys Gln Phe Val Val Cys Lys Ala Thr Pro Gly Phe

180 185 190

Ile Val Asn Arg Met Ala Arg Pro Phe Tyr Leu Glu Gly Phe Arg Leu

195 200 205

Leu Glu Glu Asn Val Ala Leu Ala Pro Gln Ile Asp Arg Ala Leu Lys

210 215 220

Ala Gly Gly His Phe Arg Met Gly Pro Leu Glu Leu Thr Asp Phe Ile

225 230 235 240

Gly Gln Asp Ile Asn Tyr Gln Val Ser Lys Gln Ile Trp Gln Asp Met

245 250 255

Gln Phe Asp Pro Arg Tyr Thr Pro Gly His Leu Gln Arg Ser Leu Val

260 265 270

Asp Ala Gly Leu Leu Gly Arg Lys Asn Gly Arg Ser Phe Phe Ala Ser

275 280 285

Gln Pro Ala Thr Pro Pro Asn Pro Thr Thr Glu Gly Asp Thr Pro Thr

290 295 300

Ser Leu His Phe Tyr Gly Glu His Ala Leu Phe Asp His Leu Gln Gln

305 310 315 320

Arg Ala Leu Ala Thr Trp Pro Ala Leu Arg Val Gln Arg Leu Pro Glu

325 330 335

Arg Pro Glu Leu Gly Arg Phe Ile Leu Met Asn Asn Arg Leu Ala Ile

340 345 350

Lys Ile Thr Asp Gly Arg Thr Ala Asn Leu Leu Ala Gly Leu Thr Ala

355 360 365

Leu Asp Thr Phe Val Ile Asp Ala Ala Leu Asn Tyr Ala Asp Thr Ala

370 375 380

Tyr Leu Val Ala Ala His Asn Gln His Ala Thr Glu Thr Asn Lys Ala

385 390 395 400

Leu Phe Leu Thr Leu Leu Gln Thr Leu Ile Ala Gln Val Glu Phe Ile

405 410 415

Lys Asp Ser Pro Ala Leu Ile Val Ala Arg Val Leu Ser Ser Leu Ile

420 425 430

Asn Glu Ser Val Ile Met Val Glu Ser Gly Val Cys Ser Arg Ala Asp

435 440 445

Ile Asp Ile Ala Ala Val Ala Gly Val Asn Tyr Ala Asp Gly Ile Phe

450 455 460

Ala Trp Leu Ala Gln Leu Gly Gln Lys Asn Val Lys Ser Thr Leu Asp

465 470 475 480

Asn Met Ala Gln Leu Leu His Ser Ala Arg Tyr Tyr Pro His Tyr Ser

485 490 495

Leu Leu Asn Ala Ala Arg Pro Glu Leu Ala Val Ala Pro

500 505

<210> 82

<211> 509

<212> PRT

<213> Serratia plymuthica 4Rx13

<400> 82

Met Ala Glu Asn Asn Ser Ala Ile Arg Ser Ala Ala Val Ile Gly Ala

1 5 10 15

Gly Thr Met Gly Arg Gly Ile Ala Tyr Leu Leu Ala Leu Asn Gly Ile

20 25 30

Arg Thr Val Leu Tyr Asn Arg Asn Gly Asn Ser Leu Asn Gln Ala Arg

35 40 45

Asp Tyr Ile Val Ser Asp Leu Asp Arg Lys Ile Asp Asn Gly Lys Ile

50 55 60

Thr Leu Gln Lys Lys Gly Gln Ile Leu Ala Asn Ile Ile Phe Ser Ala

65 70 75 80

Val Phe Asp Ala Ile Thr Asp Ser Asp Leu Val Ile Glu Thr Ile Ala

85 90 95

Glu Asp Glu Gln Thr Lys His Glu Ile Leu Ala Ala Ile Ala Ala Thr

100 105 110

Val Lys Pro Glu Ala Ile Ile Ala Thr Asn Thr Ser Ser Leu Ser Leu

115 120 125

Asn Lys Leu Ala Ala Gly Val Glu Asn Asn Pro Arg Phe Ile Gly Leu

130 135 140

His Phe Phe Asn Pro Ala Pro Leu Met Lys Leu Ile Glu Ile Ile Pro

145 150 155 160

Ser Tyr Phe Thr Ser His Ala Thr Ser Leu Arg Cys Gln Lys Leu Val

165 170 175

Ile Ala Leu Gly Lys Gln Phe Val Val Cys Lys Ala Thr Pro Gly Phe

180 185 190

Ile Val Asn Arg Met Ala Arg Pro Phe Tyr Leu Glu Gly Phe Arg Leu

195 200 205

Leu Glu Glu Asn Val Ala Leu Ala Pro Gln Ile Asp Arg Ala Leu Lys

210 215 220

Ala Gly Gly His Phe Arg Met Gly Pro Leu Glu Leu Thr Asp Phe Ile

225 230 235 240

Gly Gln Asp Ile Asn Tyr Gln Val Ser Lys Gln Ile Trp Gln Asp Met

245 250 255

Gln Phe Asp Pro Arg Tyr Thr Pro Gly His Leu Gln Arg Ser Leu Val

260 265 270

Asp Ala Gly Leu Leu Gly Arg Lys Asn Gly Arg Ser Phe Phe Ala Ser

275 280 285

Gln Pro Ala Thr Pro Pro Thr Pro Thr Thr Glu Ser Asp Thr Pro Thr

290 295 300

Ser Leu His Phe Tyr Gly Glu His Ala Leu Phe Asp His Leu Gln Gln

305 310 315 320

Arg Ala Leu Ala Thr Trp Pro Ala Leu Ser Val Gln Arg Leu Pro Glu

325 330 335

Arg Pro Glu Leu Gly Arg Phe Ile Leu Val Asn Asn Ala Leu Ala Ile

340 345 350

Lys Ile Thr Asp Gly Arg Thr Ala Asn Leu Leu Ala Gly Leu Thr Ala

355 360 365

Leu Asp Thr Phe Val Ile Asp Ala Ala Leu Asn Tyr Ala Asp Thr Ala

370 375 380

Tyr Leu Val Ala Ala His Asn Gln His Ala Thr Glu Thr Asn Lys Ala

385 390 395 400

Leu Phe Leu Thr Leu Leu Gln Thr Val Ile Ala Gln Val Glu Phe Ile

405 410 415

Lys Asp Ser Pro Ala Leu Ile Val Ala Arg Val Leu Ser Ser Leu Ile

420 425 430

Asn Glu Ser Val Ile Met Val Glu Ser Gly Val Cys Ser Arg Ala Asp

435 440 445

Ile Asp Ile Ala Ala Val Ala Gly Val Asn Tyr Ala Asp Gly Ile Phe

450 455 460

Ala Trp Leu Ala Gln Leu Gly Gln Lys Asn Val Lys Ser Thr Leu Asp

465 470 475 480

Asn Met Ala Gln Leu Leu His Ser Ala Arg Tyr Tyr Pro His Tyr Ser

485 490 495

Leu Leu Asn Ala Ala Arg Pro Glu Leu Ala Val Ala Pro

500 505

<210> 83

<211> 509

<212> PRT

<213> Serratia plymuthica V4

<400> 83

Met Ala Glu Asn Asn Ser Ala Ile Arg Ser Ala Ala Val Ile Gly Ala

1 5 10 15

Gly Thr Met Gly Arg Gly Ile Ala Tyr Leu Leu Ala Leu Asn Gly Ile

20 25 30

Arg Thr Val Leu Tyr Asn Arg Asn Gly Asn Asn Leu Asn Gln Ala Arg

35 40 45

Asp Tyr Ile Val Ser Asp Leu Asp Arg Lys Ile Asp Asn Gly Lys Ile

50 55 60

Thr Leu Gln Lys Lys Gly Gln Ile Leu Ala Asn Ile Ile Phe Ser Ala

65 70 75 80

Val Phe Asp Ala Ile Thr Asp Ser Asp Leu Val Ile Glu Thr Ile Ala

85 90 95

Glu Asp Glu Gln Thr Lys His Glu Ile Leu Ala Ala Ile Ala Ala Thr

100 105 110

Val Lys Pro Glu Ala Ile Ile Ala Thr Asn Thr Ser Ser Leu Ser Leu

115 120 125

Asn Lys Leu Ala Ala Gly Val Glu Asn Asn Pro Arg Phe Ile Gly Leu

130 135 140

His Phe Phe Asn Pro Ala Pro Leu Met Lys Leu Ile Glu Ile Ile Pro

145 150 155 160

Ser Tyr Phe Thr Ser His Ala Thr Ser Leu Arg Cys Gln Lys Leu Val

165 170 175

Ile Ala Leu Gly Lys Gln Phe Val Val Cys Lys Ala Thr Pro Gly Phe

180 185 190

Ile Val Asn Arg Met Ala Arg Pro Phe Tyr Leu Glu Gly Phe Arg Leu

195 200 205

Leu Glu Glu Asn Val Ala Leu Ala Pro Gln Ile Asp Arg Ala Leu Lys

210 215 220

Ala Gly Gly His Phe Arg Met Gly Pro Leu Glu Leu Thr Asp Phe Ile

225 230 235 240

Gly Gln Asp Ile Asn Tyr Gln Val Ser Lys Gln Ile Trp Gln Asp Met

245 250 255

Gln Phe Asp Pro Arg Tyr Thr Pro Gly His Leu Gln Arg Ser Leu Val

260 265 270

Asp Ala Gly Leu Leu Gly Arg Lys Asn Gly Arg Ser Phe Phe Ala Ser

275 280 285

Gln Pro Ala Thr Pro Pro Thr Pro Thr Thr Glu Ser Asp Thr Pro Thr

290 295 300

Ser Leu His Phe Tyr Gly Glu His Ala Leu Phe Asp His Leu Gln Gln

305 310 315 320

Arg Ala Leu Ala Thr Trp Pro Ala Leu Arg Val Gln Arg Leu Pro Glu

325 330 335

Arg Pro Glu Leu Gly Arg Phe Ile Leu Val Asn Asn Arg Leu Ala Ile

340 345 350

Lys Ile Thr Asp Gly Arg Thr Ala Asn Leu Leu Ala Gly Leu Thr Ala

355 360 365

Leu Asp Thr Phe Val Ile Asp Ala Ala Leu Asn Tyr Ala Asp Thr Ala

370 375 380

Tyr Leu Val Ala Ala His Asn Gln His Ala Thr Glu Thr Asn Lys Ala

385 390 395 400

Leu Phe Leu Thr Leu Leu Gln Thr Val Ile Ala Gln Val Glu Phe Ile

405 410 415

Lys Asp Ser Pro Ala Leu Ile Val Ala Arg Val Leu Ser Ser Leu Ile

420 425 430

Asn Glu Ser Val Ile Met Val Glu Ser Gly Val Cys Ser Arg Ala Asp

435 440 445

Ile Asp Ile Ala Ala Val Ala Gly Val Asn Tyr Ala Asp Gly Ile Phe

450 455 460

Ala Trp Leu Ala Gln Leu Gly Gln Lys Asn Val Lys Ser Thr Leu Asp

465 470 475 480

Asn Met Ala Gln Leu Leu His Ser Ala Arg Tyr Tyr Pro His Tyr Ser

485 490 495

Leu Leu Asn Ala Ala Arg Pro Glu Leu Ala Val Ala Pro

500 505

<210> 84

<211> 509

<212> PRT

<213> Serratia plymuthica 3Rp8

<400> 84

Met Ala Glu Asn Asn Ser Ala Ile Arg Ser Ala Ala Val Ile Gly Ala

1 5 10 15

Gly Thr Met Gly Arg Gly Ile Ala Tyr Leu Leu Ala Leu Asn Gly Ile

20 25 30

Arg Thr Val Leu Tyr Asn Arg Asn Gly Asn Asn Leu Asn Gln Ala Arg

35 40 45

Asp Tyr Ile Val Ser Asp Leu Asp Arg Lys Ile Asp Asn Gly Lys Ile

50 55 60

Thr Leu Gln Lys Lys Gly Gln Ile Leu Ala Asn Ile Ile Phe Ser Ala

65 70 75 80

Val Phe Asp Ala Ile Thr Asp Ser Asp Leu Val Ile Glu Thr Ile Ala

85 90 95

Glu Asp Glu Gln Thr Lys His Glu Ile Leu Ala Ala Ile Ala Ala Thr

100 105 110

Val Lys Pro Glu Ala Ile Ile Ala Thr Asn Thr Ser Ser Leu Ser Leu

115 120 125

Asn Lys Leu Ala Ala Gly Val Glu Asn Asn Pro Arg Phe Ile Gly Leu

130 135 140

His Phe Phe Asn Pro Ala Pro Leu Met Lys Leu Ile Glu Ile Ile Pro

145 150 155 160

Ser Tyr Phe Thr Ser His Ala Thr Ser Leu Arg Cys Gln Lys Leu Val

165 170 175

Ile Ala Leu Gly Lys Gln Phe Val Val Cys Lys Ala Thr Pro Gly Phe

180 185 190

Ile Val Asn Arg Met Ala Arg Pro Phe Tyr Leu Glu Gly Phe Arg Leu

195 200 205

Leu Glu Glu Asn Val Ala Leu Ala Pro Gln Ile Asp Arg Ala Leu Lys

210 215 220

Ala Gly Gly His Phe Arg Met Gly Pro Leu Glu Leu Thr Asp Phe Ile

225 230 235 240

Gly Gln Asp Ile Asn Tyr Gln Val Ser Lys Gln Ile Trp Gln Asp Met

245 250 255

Gln Phe Asp Pro Arg Tyr Thr Pro Gly His Leu Gln Arg Ser Leu Val

260 265 270

Asp Ala Gly Leu Leu Gly Arg Lys Asn Gly Arg Ser Phe Phe Ala Ser

275 280 285

Gln Pro Ala Thr Pro Pro Thr Pro Thr Thr Glu Ser Asp Thr Pro Thr

290 295 300

Ser Leu His Phe Tyr Gly Glu His Ala Leu Phe Asp His Leu Gln Gln

305 310 315 320

Arg Ala Leu Ala Thr Trp Pro Ala Leu Arg Val Gln Arg Leu Pro Glu

325 330 335

Arg Pro Glu Leu Gly Arg Phe Ile Leu Val Asn Asn Arg Leu Ala Ile

340 345 350

Lys Ile Thr Asp Gly Arg Thr Ala Asn Leu Leu Ala Gly Leu Thr Ala

355 360 365

Leu Asp Thr Phe Val Ile Asp Ala Ala Leu Asn Tyr Ala Asp Thr Ala

370 375 380

Tyr Leu Val Ala Ala His Asn Gln His Ala Thr Glu Thr Asn Lys Ala

385 390 395 400

Leu Phe Leu Thr Leu Leu Gln Thr Leu Ile Ala Gln Val Glu Phe Ile

405 410 415

Lys Asp Ser Pro Ala Leu Ile Val Ala Arg Val Leu Ser Ser Leu Ile

420 425 430

Asn Glu Ser Val Ile Met Val Glu Ser Gly Val Cys Ser Arg Ala Asp

435 440 445

Ile Asp Ile Ala Ala Val Ala Gly Val Asn Tyr Ala Asp Gly Ile Phe

450 455 460

Ala Trp Leu Ala Gln Leu Gly Gln Lys Asn Val Lys Ser Thr Leu Asp

465 470 475 480

Asn Met Ala Gln Leu Leu His Ser Ala Arg Tyr Tyr Pro His Tyr Ser

485 490 495

Leu Leu Asn Ala Ala Arg Pro Glu Leu Ala Val Ala Pro

500 505

<210> 85

<211> 509

<212> PRT

<213> Serratia proteamaculans MFPA44A14

<400> 85

Met Ala Glu Asn Asn Ser Ala Ile His Ser Val Ala Val Ile Gly Ala

1 5 10 15

Gly Thr Met Gly Arg Gly Ile Ala Tyr Leu Leu Ala Gln Asn Gly Ile

20 25 30

Arg Thr Leu Leu Tyr Asn Arg Ser Gly Asn Asn Leu Asp Gln Ala Arg

35 40 45

Asp Tyr Ile Ile Arg Asp Leu Asp Lys Lys Ile Asp Asn Gly Lys Ile

50 55 60

Ser Pro Gln Lys Lys Gly Glu Val Leu Ala Asn Leu Val Phe Ser Pro

65 70 75 80

Ile Phe Asp Ala Ile Ala Asp Ser Asp Leu Val Ile Glu Thr Ile Ala

85 90 95

Glu His Glu Thr Thr Lys His Glu Ile Leu Ala Ala Ile Ala Ala Thr

100 105 110

Val Lys Gln Glu Ala Ile Ile Ala Thr Asn Thr Ser Ser Leu Ser Leu

115 120 125

Asn Lys Leu Ala Ala Gly Val Glu Asn Asn Ala Arg Phe Ile Gly Leu

130 135 140

His Phe Phe Asn Pro Ala Pro Leu Met Lys Leu Ile Glu Ile Ile Pro

145 150 155 160

Ser Tyr Phe Thr Ser Arg Ala Thr Ser Leu Arg Cys Gln Gln Leu Val

165 170 175

Thr Ala Leu Gly Lys Gln Phe Val Val Cys Lys Ala Thr Pro Gly Phe

180 185 190

Ile Val Asn Arg Met Ala Arg Pro Phe Tyr Leu Glu Gly Phe Arg Leu

195 200 205

Leu Glu Glu Asn Val Ala Leu Ala Pro Gln Ile Asp Arg Ala Leu Lys

210 215 220

Ala Gly Gly His Phe Arg Met Gly Pro Leu Glu Leu Thr Asp Phe Ile

225 230 235 240

Gly Gln Asp Ile Asn Tyr Gln Val Ser Ser Gln Ile Trp Gln Asp Met

245 250 255

Gln Tyr Asp Pro Arg Tyr Thr Pro Gly His Leu Gln Arg Ser Leu Val

260 265 270

Asp Ala Gly Leu Leu Gly Lys Lys Asn Gly Arg Ser Phe Phe Ala Ala

275 280 285

Pro Ser Ala Glu Ser Asn Leu Leu Asp Ala Gly Asn Gly Thr Leu Thr

290 295 300

Ser Leu His Phe Tyr Gly Glu His Thr Leu Phe Asp Leu Leu Gln Gln

305 310 315 320

Arg Ala Leu Ala Thr Trp Pro Thr Leu Gln Ile Ile His Gln Pro Glu

325 330 335

Arg Pro Thr Leu Gly Arg Phe Ile Arg Val Asn Asp Ala Leu Ala Val

340 345 350

Lys Ile Thr Asp Gly Arg Thr Ala Asn Leu Leu Ala Glu Leu Thr Asp

355 360 365

Leu Asp Thr Phe Val Ile Asp Ala Ala Leu Asn Tyr Ser Asp Thr Thr

370 375 380

Tyr Leu Val Ala Ala His Asn Gln Asp Ala Ala Glu Ala Asn Lys Ala

385 390 395 400

Leu Phe Leu Ser Leu Leu Gln Thr Leu Ile Pro Gln Val Glu Phe Ile

405 410 415

Lys Asp Ser Pro Gly Leu Ile Val Ala Arg Val Leu Ser Ser Leu Ile

420 425 430

Asn Glu Ser Val Ile Met Val Glu Ser Gly Val Cys Ser Arg Ala Asp

435 440 445

Ile Asp Ile Ala Ala Val Ala Gly Val Asn Tyr Ala Asp Gly Ile Phe

450 455 460

Ala Trp Leu Thr Gln Leu Gly Gln Lys Asn Val Lys Ser Thr Leu Asp

465 470 475 480

Asn Met Ala Gln Leu Leu His Ser Ala Arg Tyr Tyr Pro His Tyr Ser

485 490 495

Leu Leu Asn Ile Pro Arg Pro Glu Leu Ala Val Ala Pro

500 505

<210> 86

<211> 509

<212> PRT

<213> Serratia plymuthica A153

<400> 86

Met Ala Glu Asn Asn Ser Ala Ile Arg Ser Ala Ala Val Ile Gly Ala

1 5 10 15

Gly Thr Met Gly Arg Gly Ile Ala Tyr Leu Leu Ala Leu Asn Gly Ile

20 25 30

Arg Thr Val Leu Tyr Asn Arg Asn Gly Asn Asn Leu Asn Gln Ala Arg

35 40 45

Asp Tyr Ile Val Ser Asp Leu Asp Arg Lys Ile Asp Asn Gly Lys Ile

50 55 60

Thr Leu Gln Lys Lys Gly Gln Ile Leu Ala Asn Ile Ile Phe Ser Asp

65 70 75 80

Val Phe Asp Ala Ile Thr Asp Ser Asp Leu Val Ile Glu Thr Ile Ala

85 90 95

Glu Asp Glu Gln Thr Lys His Glu Ile Leu Ala Ala Ile Ala Ala Thr

100 105 110

Val Lys Pro Glu Ala Ile Ile Ala Thr Asn Thr Ser Ser Leu Ser Leu

115 120 125

Asn Lys Leu Ala Ala Gly Val Glu Asn Asn Pro Arg Phe Ile Gly Leu

130 135 140

His Phe Phe Asn Pro Ala Pro Leu Met Lys Leu Ile Glu Ile Ile Pro

145 150 155 160

Ser Tyr Phe Thr Ser Arg Ala Thr Ser Leu Arg Cys Gln Gln Leu Val

165 170 175

Thr Ala Leu Gly Lys Leu Phe Val Val Cys Lys Ala Thr Pro Gly Phe

180 185 190

Ile Val Asn Arg Met Ala Arg Pro Phe Tyr Leu Glu Gly Phe Arg Leu

195 200 205

Leu Glu Glu Asn Val Ala Leu Ala Pro Gln Ile Asp Arg Ala Leu Lys

210 215 220

Ala Gly Gly His Phe Arg Met Gly Pro Leu Glu Leu Thr Asp Phe Ile

225 230 235 240

Gly Gln Asp Ile Asn Tyr Gln Val Ser Lys Gln Ile Trp Gln Asp Met

245 250 255

Gln Phe Asp Pro Arg Tyr Thr Pro Gly His Leu Gln Arg Ser Leu Val

260 265 270

Asp Ala Gly Leu Leu Gly Arg Lys Asn Gly Arg Ser Phe Phe Ala Ser

275 280 285

Gln Pro Ala Thr Pro Pro Asn Pro Thr Thr Glu Gly His Thr Pro Thr

290 295 300

Ser Leu Leu Phe Tyr Gly Glu His Ala Leu Phe Asp His Leu Gln Gln

305 310 315 320

Arg Ala Leu Ala Thr Trp Pro Ala Leu Arg Val Gln Arg Leu Pro Glu

325 330 335

Arg Pro Glu Leu Gly Arg Phe Ile Leu Val Asn Asn Arg Leu Ala Ile

340 345 350

Lys Ile Thr Asp Gly Arg Thr Ala Asn Leu Leu Ala Gly Leu Thr Ala

355 360 365

Leu Asp Thr Phe Val Ile Asp Ala Ala Leu Asn Tyr Ala Asp Thr Ala

370 375 380

Tyr Leu Val Ala Ala His Asn Gln His Ala Thr Glu Pro Asn Lys Ala

385 390 395 400

Leu Phe Leu Thr Leu Leu Gln Thr Leu Ile Ala Gln Val Glu Phe Ile

405 410 415

Lys Asp Ser Pro Ala Leu Ile Val Ala Arg Val Leu Ser Ser Leu Ile

420 425 430

Asn Glu Ser Val Ile Met Val Glu Ser Gly Val Cys Ser Arg Ala Asp

435 440 445

Ile Asp Ile Ala Ala Val Ala Gly Val Asn Tyr Ala Asp Gly Ile Phe

450 455 460

Ala Trp Leu Ala Gln Leu Gly Gln Lys Asn Val Lys Ser Thr Leu Asp

465 470 475 480

Asn Met Ala Gln Leu Leu His Ser Thr Arg Tyr Tyr Pro His Tyr Ser

485 490 495

Leu Leu Asn Ala Ala Arg Pro Glu Leu Ala Val Ala Pro

500 505

<210> 87

<211> 1530

<212> DNA

<213> Serratia marcescens ATCC13880

<400> 87

atggcagaaa gtaatgcggc aattcaatcg gctgcgatta tcggcgcggg aacgatgggc 60

agaggcatcg cttatctttt cgcgcaaaaa ggcattcgca cggtgcttta taatcgcaac 120

ggcaataccc tcaatcaggc tcgcgaatat atcgcgcaag acctgaacaa gaaagtcgaa 180

cagggcaaga tcgcgctgca ggataaaggc gcggtgctgg ccaatctaat gttcacttca 240

gtgtttgagg ccatcgccga cagcgagctg gtgatagaaa ccatcgccga gcaagaacaa 300

accaaacttg aggtgctggc ggccatcgcc gcggtggtca agcccgacac gctgatcgcc 360

accaatacct cctcactgtc gcttaacaag ctggctactg cggtgacgca cagcgaacgc 420

tttatcggtt tgcatttttt caaccccgcg ccgctgatga agctgattga aatcattccg 480

gcctacttta ccgcgcacgc caccaccgaa cgctgccgcc aactggtggc ggcgttgggg 540

aaacacgatg tcgtctgcca ggccacgccg gggttcatcg tcaatcgcat ggcccgcccc 600

tactacctgg aagggttccg cctgttggaa gaacacgtgg cgcgcgcggc tcagatcgac 660

cgcgccctca aggccggcgg gcgcttccgc atggggccgc tcgagctgac cgattttatc 720

ggccaagaca tcaactatca ggtcagtcgg caaatctggc aggatatgca atacgacccg 780

cgctataccc ccggtcatct gcagcgttca ctggtcgatg ccggtctgtt ggggaaaaag 840

aacggccgct cctattttgc cgccgaagaa accgccccgc cggtgacggc cgccagcaat 900

gcagacgtcg agacgctgcg cgtttacggc gagcaccctt tttttaccct gttacagcag 960

cgagccgcgc ttcagtggcc acagctgcgc gtggaacaac ggccggcatt accggggctg 1020

gggtcggccg tccagatcaa tgacgctttc accgtcagca tcaccgatgg ccgcacggcg 1080

agccaactgg ccgagcagac ggcagcggat gcctttgtgg tcgatgtcgc cctgaactac 1140

gccgacacga cgtatctggc ggcggcgcac agccgccacg cctctgcggc caataaggcg 1200

ctgtttttac gcctgctgca cacggcaatc ccgcaggttg aatttatcaa ggactctccg 1260

gcgcttatcg tcgcccgcgt cctcagcagc ctgatcaatg agtcggtgat catggtggaa 1320

agcggcgtct gcagccggga agacatcgat gtcgccgccg tcgcgggcgt taactacgcc 1380

ggcggcattt tcgactggct cggcaaactg ggggagaaaa acgtcaggac aacgctgagc 1440

aatctggctc agctgctgca cgcggcgcgc tatgcgccgc attacaccct tctgcacgcc 1500

gcgcaaccgg cgctgacgac cacgccttaa 1530

<210> 88

<211> 1530

<212> DNA

<213> Serratia nematodiphila DSM21420

<400> 88

atggcagaaa gtaatgcggc aattcaatcg gctgcgatta tcggcgcggg aacgatgggc 60

agaggcatcg cttatctttt cgcgcaaaaa ggcattccca cgatgcttta taatcgcaac 120

ggcaataccc tcaatcaagc tcgcgaatat atcgcgcaag acctgaataa gaaagtcgaa 180

cagggcaaga tcgcgctgcg ggataaagac gcggtgctgg ccaatctgat gttcacttcc 240

gtgtttgagg ccatcgccga cagcgagctg gtgatagaaa ccatcgccga gcaagaacaa 300

accaaacttg aggtgctggc ggccatcgcc gcagtggtca agcccgacac gctgatcgcc 360

accaatacct cctcactgtc gctcaacaag ctggcaacgg cggtaacgca cagcgaacgc 420

tttatcggtt tgcatttttt caaccccgcg ccgctgatga agctgattga aatcattccg 480

gcctacttta ccgcgcacgc caccacggaa cgctgccgcc aactggtggc ggcgttgggg 540

aaacgcgatg tcgtctgcca ggccacgccg gggttcatcg tcaatcgcat ggcccgcccc 600

tactacctgg aagggttccg cctgttggaa gaacatgtgg cgcgcgcggc gcagatcgac 660

cgcgccctca aagccggcgg gcgcttccgc atggggccgc tcgagctgac cgattttatc 720

ggccaagaca tcaactatca ggtcagccgg caaatctggc aggacatgca atacgacccg 780

cgctataccc ctggtcatct gcagcgttca ctggtcgatg ccggtctgtt ggggaaaaag 840

aacggccgct cctattttgc cgccgaagaa accgccccgc cggtgacggc cgccaacaat 900

gcagacgtcg agacgctgcg cgtttacggc gagcatcctt tttttaccct gttgcagcag 960

cgagccgcgc ttcagtggcc acagctgcgc gtggaacaac ggccggcatt accggggctg 1020

ggagcggccg tccagatcaa tgacgctttc accgtcagca tcaccgatgg ccgcacggcg 1080

agccaactgg ccgagcagac ggcagcggat gcctttgtgg tcgatctcgc cctgaactac 1140

gccgacacca cgtatctggt ggcggcgcac agccgccacg cttctgcggc caataaggcg 1200

ctgtttttac gcctgctgca cacggcaatc cctcaggttg aatttatcaa ggactctccg 1260

gcgcttatcg tcgcccgcgt cctcagcagc ctgatcaatg agtcggtgat catggtggaa 1320

agcggcgtct gcagccggga agacatcgat gtcgccgccg tcgcgggcgt taactacgcc 1380

ggcggcattt tcgactggct cggcaaactg ggggagaaaa acgtcaggac gacgctgagc 1440

aatctggcgc agctgctgca cgcggcgcgc tatgcgccgc attacaccct tctgcacgcc 1500

gcgcaaccgg cgctgacgac cacgccttaa 1530

<210> 89

<211> 1530

<212> DNA

<213> Serratia plymuthica NBRC102599

<400> 89

atggcagaga ataattcggc aatccgttca gccgccgtta ttggtgcggg gaccatgggc 60

agaggcatcg cctatctcct ggcgctgaac ggcatacgaa ccgtacttta taatcgcaat 120

ggtaataatc tcaatcaggc ccgtgactat attgtcagcg acctggacag aaaaatagat 180

aacggaaaaa taaccctgca gaaaaaaggc cagatattag ccaatattat tttctcggac 240

gtctttgacg ccataaccga cagcgatctg gtgattgaaa ccattgcgga agatgagcaa 300

accaagcatg aaatcctggc agccattgcg gcgacggtaa aaccggaggc gatcattgcg 360

accaatactt cctctctgtc gctgaacaaa ctggcggcgg gggtggaaaa caacccgcgc 420

tttatcggcc tgcatttttt caatccggcg ccgctgatga agttgatcga aattattccc 480

tcttatttca cctctcgcgc caccagtcta cgctgccagc agttggtaac agcgttgggt 540

aaacagtttg tggtctgcaa agccacgccg ggctttattg ttaaccgcat ggcgcggcct 600

ttctatctgg aagggttccg gctgctggag gaaaacgtgg cgctggcgcc acagatcgac 660

cgcgccctca aggccggcgg gcattttcgc atggggcctt tagaactgac tgattttatc 720

ggccaggata tcaactatca ggtcagcaag cagatttggc aggatatgca gttcgatccc 780

cgctataccc ccggccattt gcaacgctcg ctggtggatg ccgggctgct ggggaggaaa 840

aacgggcgct ctttttttgc ttcccaacct gctacaccac ccaacccaac cacagagggc 900

gacacgccaa cttcactgca tttttatggt gaacacgctt tattcgatca cctgcaacag 960

cgcgctttgg ccacctggcc tgcgctgcgc gttcagcggt tgccggaacg gccggaactg 1020

gggcgtttta tcctggtgaa taacaggctg gcgatcaaaa tcactgatgg cagaacggcg 1080

aacctgctcg ccggcttaac cgctctcgac accttcgtga ttgacgccgc gctgaactac 1140

gccgacaccg cctatctggt ggccgcccac aatcaacatg ccacagagac gaataaagcg 1200

ctgtttctga cgctgctgca aaccctcatc gctcaggtgg agtttattaa agattcccct 1260

gccctgatcg ttgcccgcgt actgagcagc ctgatcaatg aatcggtgat catggtggag 1320

agcggcgttt gcagccgggc agatatcgat atcgccgccg tggccggcgt gaactatgcc 1380

gacggcattt ttgcctggtt ggcgcagctc gggcagaaaa acgtgaaatc gacgctggat 1440

aacatggcgc aattgctgca ctccacgcgc tattacccgc attactcatt gctgaacgcg 1500

gcccggcctg agctggctgt agcgccctaa 1530

<210> 90

<211> 1530

<212> DNA

<213> Serratia proteamaculans 568

<400> 90

atggcagaga ataattcggc aatccattcg gtcgctgtta ttggtgccgg gaccatgggc 60

agaggtattg cctatcttct ggcgcaaaac ggcatacgaa ccctgcttta taatcgtagc 120

ggtaataatc tgaatcaggc cagggactat attattcgtg acctggataa gaaaatagat 180

ggcggaaaaa taagcccgca gaaaaaaggc gagatattgg ccaatctggt tttctctccc 240

attttcgagg ctattgccga cagcgatctg gtgattgaaa ccatcgcgga acatgaggca 300

accaagcatg agatcctcgc ggcgattgcg gccacggtga aaaaagaggc gattatcgcc 360

accaatacct cctcgctgtc attgaataag ctggccgcag gcgtcgaaaa taacgcccgg 420

tttatcggcc tgcacttctt caatccggcc ccgctgatga aactgatcga aattattccg 480

tcttacttta ccagccgggc caccagcctg cgttgccagc agttggtgac ggcgataggc 540

aaacagtttg tggtctgcaa agccacgccg ggctttatcg tcaaccggat ggcgcgacct 600

ttttatctgg aagggttccg gctgttggaa gagaacgtgg cgctggcgcc gcagatcgac 660

cgcgcactca aggccggtgg ccactttcgc atgggacctt tagagctgac cgattttatc 720

ggccaggata ttaactatca ggtcagcagc cagatttggc aggacatgca gtacgatccc 780

cgctataccc ccggccattt gcaacgttcg ctggtggatg ccgggctgct ggggaagaaa 840

aacggccgat ccttttttac cccctcttcc gccgaaccca gctccgccga tgcaggtagc 900

ggcacgccga cctcactgaa tttttatggt gaacatcccc tgttcgacct gttgcaacag 960

cgcgctttgg cgctctggcc aagggtgcag attaatcgcc aatcggaaca gccaacgctg 1020

ggccgcttta tccgggtgaa tgacgcaatg gccatcaaaa tcaccgatgg ccgcaccgcc 1080

aatctgctgg ctgaattgac cgaactcgat accttcgtga tcgacgccgc gctcaactac 1140

gccgataccg cctatctggc ggctgcccac agccaggatg ccagcgcggc caataaagcg 1200

ctgtttctga cgctgctgca aacgttgatc ccgcaggtgg agtttattaa agactccccg 1260

ggcctgattg tcgcccgcgt cctgagcagc ctgatcaatg agtcggtgat tatggtggag 1320

agcggggttt gcagccgggc ggacatcgat atcgccgcgg tggccggcgt taactatgcc 1380

gatggcatct ttagctggct ggcgcagctt gggcaaaaaa acgtgaagtc gacgctggac 1440

aatatggcgc aactgctgca ttccgcccgc tattacccgc attactcttt gctcaatacc 1500

ccccggccag agctggccgt cgcgccctaa 1530

<210> 91

<211> 1530

<212> DNA

<213> Serratia ureilytica Lr5/4

<400> 91

atggcagaaa gtaatgcggc aattcaatcg gctgcgatta tcggcgcggg aacgatgggc 60

agaggcatcg cttatctttt ggcgcaaaaa agcattcgta cggtgcttta taatcgcaac 120

ggcaataccc tcaatcaagc tcgcgacgct atcgtgcaag acctgaacaa aaaggtggaa 180

cagggcaagc tcgcgctgca ggacaaagac gcggtgctgg ctaatctgac gttctccgcg 240

gaatttgggg ccatcgccga cagcgatctg gtgatcgaaa ccatcgcaga gcatgagcaa 300

gccaaactcg aggtgctggc ggccatcgcc gcgacggtca agcccgacac gctgatcgcc 360

accaatacct cctcactgtc gcttaataag ctggcgacgg cggtgacgca cagcgaacgc 420

tttatcggtt tgcacttttt caaccccgcg ccgctgatga agctgattga aatcattccg 480

gcctatttta ccgcacaggt caccaccgaa cgttgccgtc aactggtggc ggcgttgggg 540

aaacgcgatg tcgtctgtca ggccacgccg ggatttatcg tcaaccgtat ggcccgcccc 600

tactatctgg aagggttccg cctgttggaa gaacacgtgg cgcgtgcgcc gcagatcgac 660

cgcgccctca aagccggcgg acactttcgc atggggccgc tcgaactgac cgattttatc 720

ggtcaggaca tcaactatca ggtcagccgg caaatttggc aggacatgca gtacgatccg 780

cgctataccc cgggccatct gcagcgctcg ctggtcgatg ccggtctgtt gggcaaaaag 840

aacggccgct cctatttttc cgccgaagaa tctcctccgc cgcttgcggc cgccgtcgat 900

gcggaggtcg agacgctacg catttacggt gaacatcctc tctttactct gctacagcag 960

cgggccaccc tgcaatggcc gcggctgcgc gtggaacaac ggccgacatt accgggcctg 1020

ggcgccgcca ttcaggttaa tgacgctttc accgtcagcg ttaccgatgg ccgcacggca 1080

aaccagcttg ccgaacagac cgcggcggac gcctttgtcg tcgatgtcgc cctgaactac 1140

ggcgatacgg cgtacctggt ggcggcgcat agccgccacg cctctgcagc caataaggcg 1200

ctgtttttac gcctgctgca caccgcgatc ccgcaggtgg aatttatcaa ggattccccg 1260

gccttgatcg tcgcccgcgt gctcagcagc ctgatcaacg agtcggtgat catggtggaa 1320

agcggcgtct gcagccggga agacattgat gtcgccgccg tcgccggcgt gaactacgcc 1380

gacggtattt tcggctggct cactcgcctc ggggaggaaa atgtcaggac gacgctgagc 1440

aacctggcgc aattgctgca cgcggcgcgc tatgcgccgc attacaccct tctgcacgcc 1500

gcccaaccgg cgctgaccac cacgccttaa 1530

<210> 92

<211> 1530

<212> DNA

<213> Serratia sp. BW106

<400> 92

atggcagaga ataattcggc aatccattcg gtcgctgtta ttggtgccgg aaccatgggc 60

agaggtattg cctatcttct ggcgcagaac ggcatacgaa ccctgcttta taatcgtagc 120

ggtaataatc tggatcaggc ccgcgactat attatccgtg acctggataa gaaaatagac 180

aacgggaaaa taagccaaca gaaaaaaggc gaagtattgg ccaacctggt tttctctccc 240

attttcgacg ctatcgccga cagcgacctg gtgattgaaa ccatcgcgga gcatgagacc 300

accaagcatg aaatcctcgc ggcgattgcg gccacggtga aacaagaggc cattatcgcc 360

accaacacct catcgctgtc gttgaataag ctggcggcag gcgtcgaaaa caacgcccgt 420

tttatcggcc tgcacttctt caatccggcc ccgctgatga aactgatcga gattattccg 480

tcctatttta ccagccgggc caccagcctg cgctgccagc agttggtgac ggcgctaggc 540

aaacagtttg tggtctgcaa agccacgccg ggttttatcg tcaaccggat ggcacggcct 600

ttttatctgg aaggattccg gctgttggaa gaaaacgtag cattggcacc gcagatcgac 660

cgcgccctca aggccggtgg ccactttcgc atgggccctt tagagctgac cgactttatc 720

ggccaggata ttaactatca ggtcagcagc cagatttggc aggacatgca gtacgaccct 780

cgctataccc ccggccattt gcaacgttcg ctggtggatg ccgggttgtt ggggaagaaa 840

aacggccgat ccttttttgc tgccccttct tccgaatcga accccctcga cgcaggcaac 900

ggcacgctga cttccctgca tttttatggc gaacataccc tgtttgacct gctgcaacag 960

cgcgccttgg ctatctggcc aacgctgcag attattcacc agccggaacg gccgacgctg 1020

ggacgcttta tccgggtgaa tgacgcattg gccgtcaaaa tcaccgatgg tcgcaccgcc 1080

aatctgctcg ctgaattgac cgatctcgac acctttgtga tcgacgccgc actgaattac 1140

agcgataccg cctatctggt ggccgcccac aatcaggacg ccgccgaggc caataaagcg 1200

ctgtttctgt cgctgctgca aacgttgatc ccgcaggtgg agtttattaa agactctcca 1260

ggcctgatcg tcgcccgggt tctgagcagt ctgatcaatg agtcggtgat catggtggag 1320

agcggggttt gcagccgggc agatatcgat attgccgccg tggcgggcgt taactatgcc 1380

gatggcatct ttgcctggct gacgcagctc gggcaaaaaa acgtgaaatc aacgctggat 1440

aatatggcgc aactgctgca ttccgcccgc tattacccgc attactcatt gctgaatgcc 1500

ccccggcccg aactggccgt cgcgccgtaa 1530

<210> 93

<211> 1530

<212> DNA

<213> Serratia liquefaciens FK01

<400> 93

atggcagaga ataatacggc aatagattcg gtcgccgtga ttggcgccgg aaccatgggc 60

agaggcattg cctatctttt ggcactgaac ggcatacgaa ccctgcttta taatcgaaac 120

ggtaatcatc ttaaccaggc ccgggattat attgtcggcg acctggataa aaaaatcgac 180

aacggaaaaa taagccaaca gaaaaagggc gaggtcctgg ccaatctggt tttctcgcca 240

gttttcgacg ccattgccga cagtgacctg gtgattgaaa ccatagcgga acatgaaccc 300

accaagcatg agatcctcgc ggccattgcc gccacggtaa aaaaagaggc gattatcgcc 360

accaatacct cttcgctgtc gctgaataaa ctggccgcag ggatagaaaa caactcgcgc 420

tttatcggcc tgcatttctt caacccggca ccgctgatga agctgatcga aattatcccg 480

tcctatttta ccgcccgggc caccacctta cgctgccagc agttggtcac ggcgataggc 540

aaaaaatttg tggtctgcaa agccacgccg ggctttatcg tcaatcgcat ggcgcggcct 600

ttttatctgg aaggttttcg cctgctggag gaaaacgtgg cgctggcacc gcaaatcgac 660

cgcgcgctca aggccggagg ccacttccgc atggggcctt tggaactgac tgattttatt 720

ggtcaggaca ttaattatca ggtcagcagc cagatttggc aggacatgca gtatgacccc 780

cgctataccc ccggccattt gcaacgttcg ctggtggatg ccgggctgtt ggggaagaaa 840

aacgggcgct cttttttttc cccctcggct gacgccgcca acccaccggc taccggcggc 900

ggtacgctga gctcgctgca tttttttggc gaacatccgc tgtttgatct gctgcaacag 960

cacgccttcg caacctgggc gcccctggca ataacacgtc agccggaaca cccggttctg 1020

ggccgtttta tccaggtgaa tgacagtctg gcagtcaaaa tcaccgacgg gcgaacggcc 1080

aatcagcttg ccgagctggc gggtctcgat accttcgtgg tcgacgttgc actgaactat 1140

gccaacaccg cttttctggt ggcggcccac agccaacagg ctaccgaggc gaataaagag 1200

ctgttcctca cgctgctgca aacggtgatc ccgcaggtgg agtttgttaa agattcccca 1260

ggcctgatcg tcgcccgggt tctgagcagc ctgatcaatg agtcggtgat catggtggag 1320

agcggggttt gtagccgaga agacatcgat atcgccgccg tggccggcgt caactatgcc 1380

gacggcattt ttgcctggct ggcgcagctc gggcagaaaa acgtgaaatc gacgctggat 1440

aatatggcgc aactgctgca ttccgcccgc tattacccgc actactcgtt gctccacgct 1500

accaggcccg agctggccgt cgcgccatga 1530

<210> 94

<211> 1530

<212> DNA

<213> Serratia sp. S119

<400> 94

atggcagaaa gtaatgcggc aattcaatcg gctgcgatta tcggcgcggg aacgatgggc 60

agaggcatcg cttatctttt cgcgcaaaaa ggcattcgca cggtgcttta taatcgcaac 120

ggcaataccc tcaatcaggc tcgcgaatat atcgcgcaag acctgaacaa gaaagtcgaa 180

cagggcaaga tcgcgctgca ggataaaggc gcagtgctgg ctaatctggt gttcacctcg 240

gtgtttgaga ctatcgccga cagcgatcta gtgatagaaa ccatcgccga gcaagagcaa 300

accaaacttg aggtgctggc ggccatcgcc gcggcggtca agcccgacac gctgatcgcc 360

accaatacat cctcactgtc gcttaacaag ctggcaaccg cggtgacaca cagcgagcgg 420

tttatcggtt tgcacttttt caatcccgcg ccgctgatga aactgattga aatcatcccg 480

gcctacttta ccgcacgcgc caccaccgaa cgttgccgtc agctggtggc cgcgttgggg 540

aaacgcgatg tcgtctgcca ggccacgccg gggtttatcg tcaaccgcat ggcccgcccc 600

tactacctgg aagggttccg cctgttggaa gaacacgtgg cgcgcgcgcc gcagatcgac 660

cgcgccctca aggccggcgg gcactttcgc atggggccgc tcgagctgac cgattttatc 720

ggccaggaca tcaactatca ggtcagccgg caaatctggc aggacatgca atacgatccg 780

cgctataccc ccggccatct gcagcgttcg ctggtcgatg ccggtctgtt ggggaaaaag 840

aacggccgct cctattttgc cgccgaagaa aacgccttac cggtaacggc cgccaccgat 900

gcagacgtcg agacgctgcg cgtttacggc gaacaccctt tttttaccct gttgcaacag 960

cgggccgcgc ttcagtggcc acggctgcgc gtggagcaac ggccggcctt accgggcctg 1020

gggccggccg tccagatcaa tgaggctttc accgtcagcg tcaccgatgg ccgcacggcg 1080

agccagttgg cggagctgac ggcggcggat gcctttgtgg tcgatgtcgc cctgaactac 1140

gccgacacgg cgtatctggt ggcagcgcac aaccgccacg cctctgcggc caataaggcg 1200

ctgtttttac gcctgctgca cacggcaatc ccgcaggttg aatttatcaa ggactccccg 1260

gcgctgatcg tcgcccgcgt cctcagcagc ctgatcaacg agtcggtgat catggtggaa 1320

agcggcgtct gcagccggga agacatcaat gtcgccgccg tcgccggcgt taactacgcc 1380

gacggcattt tcggcttgct cgatagcctg ggggagaaaa acgtcaggac gacgctgagc 1440

aacctggcgc agctgctgca cgcggcacgc tatgcgccgc attacaccct tctacacgcc 1500

gcgcaaccgg cgctgacgac cacgccttaa 1530

<210> 95

<211> 1530

<212> DNA

<213> Serratia sp. YD25

<400> 95

atggcagaac gtaatgcggc aattcaatcg gctgcgatta tcggcgcggg aacgatgggc 60

agaggcatcg cttatctttt cgcgcaaaaa ggcattcgta cggtgcttta taatcgcaac 120

ggcaataccc tcattcaggc tcgtgaatat atcgtgcaag acctggacaa aaaggtcgaa 180

cagggcaggc tcgcgccgca ggataaagac gcggtgctgg ccaatctgca gttctcatcg 240

gtgtttgaag ctatcgtcga cagcgatttg gtgctcgaaa ctatcgccga gcaagagcaa 300

gccaaactcg aggtgctggc cgccatcgct gcggcggtca aacccgacac gctgatcgcc 360

accaatactt cctcattgtc gctcaataag ctggcaacgg cggtgaccca cagcgaacgc 420

tttatcggtt tgcacttttt caatcccgca ccgctaatga agctgattga aatcattccg 480

gcctacttta ccacccaagc caccactgaa cgttgccgcc aactggtggc ggcattgggg 540

aaacgcgatg tcgtctgcca ggctacgccg gggttcatcg tcaaccgtat ggcccgcccc 600

tactacctgg aaggctttcg cctgttggaa gagcacgtgg cgcgcgcgcc gcagatcgac 660

cgcgccctca aggccggcgg acactttcgc atggggccgc tcgagctgac cgattttatc 720

ggccaggaca tcaactatca ggtcagccgg caaatttggc aggacatgca gtacgacccg 780

cgctataccc ccggccatct gcagcgttcg ctggtcgatg ccgggctgct ggggaaaaag 840

aacggtcgct cctatttttc cgccgaagaa accgccccgc cggttgaggc cgccgccgag 900

gcggatgtcg agacgctgcg catttacggc gaacaccctt tgtttaccct gctgcaacag 960

cgagccgcac tgcaatggcc gcagttgcgc gtggaacagc ggccggcctt gtcgggcctg 1020

ggagcggcca ttcaggtcaa tgacgctttc accgtcagcg tcaccgatgg ccgcacggca 1080

aatcagttgg ccgagcagac ggcggcggac gcctttgtgg tcgatgtcgc cctgaactac 1140

gccgacacgg cgtatctggt ggcggcgcac agccgccacg cctctgcggc taataaggcg 1200

ttgtttttgc gcctgctgca caccgcgctt ccgcaggttg aatttatcaa ggactccccg 1260

gcgctgatcg tcgcccgcgt cctcagcagc ctgatcaacg agtcggtgat catggtggaa 1320

agcggcgtct gcagccggga agacatcgat gtcgccgccg tcgccggcgt gaactacgcc 1380

gacggcattt tcggctggct cactcgcctg ggggagaaaa acgtcaggac gacgctgagc 1440

aacctggcgc agctgctgca cgcggcccgc tatgcgccgc attacaccct tctgcacgcc 1500

gcccaaccgg cgctgaccac cacgccttaa 1530

<210> 96

<211> 1530

<212> DNA

<213> Serratia sp. FS14

<400> 96

atggcagaaa gtaatgcggc aattcaatcg gctgcgatta tcggcgcggg aacgatgggc 60

agaggcatcg cttatctttt cgcgcaaaaa ggcattccca cgatgcttta taatcgcaac 120

ggcaataccc tcaatcaagc tcgcgaatat atcgcgcaag acctgaacaa gaaagtcgaa 180

cagggcaaga tcgcgttgca ggataaagac gcggtgctgg ccaatctgat gttcacttcc 240

gtgtttgagg ccatcgccga cagcgagctg gtgatagaaa ccatcgccga gcaagaacaa 300

accaaacttg aggtgctggc ggccatcgcc gcggtggtca agcccgacac gctgatcgcc 360

accaatacct cctcactgtc gcttaacaag ctggcaactg cggtgacgca cagcgagcgc 420

tttatcggtt tacatttttt caaccccgct ccgctgatga agctgattga aatcattccg 480

gcctacttta ccgcgcacgc caccacggaa cgctgccgcc aactggtggc ggcgttgggg 540

aaacgcgatg tcgtctgcca ggccacgccg gggttcatcg tcaatcgcat ggcccgcccc 600

tactacctgg aagggttccg cctgttggaa gaacatgtgg cgcgcgcggc gcagatcgac 660

cgcgccctca aggccggcgg gcgcttccgc atggggccgc tcgagctgac cgattttatc 720

ggccaagaca ttaactatca ggtcagccgg caaatctggc aggacatgca atacgacccg 780

cgctataccc ctggtcatct gcagcgttca ctggtcgatg ccggtctgtt ggggaaaaag 840

aacggccgct cctattttgc cgccgaagaa accgccccgc cggtgacggc cgccaacaat 900

gcagacgtcg agacgctgcg cgtttacggc gagcatcctt tttttaccct gttgcagcag 960

cgagccgcgc ttcagtggcc acagctgcgc gtggaacaac ggccggcatt accggggctg 1020

ggagcggccg tccagatcaa tgacgctttc accgtcagca tcaccgatgg ccgcacggcg 1080

agccaactgg ccgagcagac ggcagcggat gcctttgtgg tcgatgtcgc cctgaactac 1140

gccgacacca cgtacctggt ggcggcgcac agtcgccacg cttctgcggc caataaggcg 1200

ctgtttttac gcctgctgca cacggcaatc ccacaggttg aatttatcaa ggactctccg 1260

gcgcttatcg tcgcccgcgt cctcagcagc ctgatcaatg agtcggtgat catggtggaa 1320

agcggcgtct gcagccggga agacatcgat gtcgccgccg tcgcgggcgt taactacgcc 1380

ggcggcattt tcgactggct cggcaaactg ggggagaaaa acgtcaggac gacgctgagc 1440

aatctggcac agctgctgca cgcggcgcgc tatgcgccgc attacaccct tctgcacgcc 1500

gcgcaaccgg cgctgacgac cacgccttaa 1530

<210> 97

<211> 1530

<212> DNA

<213> Serratia sp. HMSC15F11

<400> 97

atggcagaaa gtaatgcggc aattcagtcg gctgcgatta tcggcgcggg aacgatgggc 60

agaggcatcg cttatctttt cgcgcaaaaa ggcattcgca cggtgcttta taatcgcaac 120

ggcaataccc tcaatcaggc tcgcgactat atcgagctag acctgaacaa gaaagtcgaa 180

cagggtaaga tcgcgctgca ggataaaggc gcggtgttgg ccaatctggt gttcacctcg 240

gtatttgaga ccattgccga cagcgagctg gtgatagaaa ccatcgccga gcaagagcaa 300

accaaacttg aggtgctggc ggccatcgcc gcggcagtca agcccgacac gctgatcgcc 360

accaatacct cctcactgtc gctgaataag ctggcaaccg cggtgacgca cagcgagcgg 420

tttatcggtt tgcacttttt taaccccgcg ccgctgatga aactgattga aatcatcccg 480

gcctacttta ccgcacacgc caccacggag cgttgccgtc aactggtggc cgcgttgggg 540

aaacgcgatg ttgtctgcca ggccacgccg gggtttatcg tcaaccgcat ggcccgcccc 600

tactacctgg aagggttccg cctattggaa gaacacgtgg cgcgcgcgcc gcagatcgac 660

cgtgccctca aggccggcgg gcactttcgt atggggccgc tcgagctgac cgattttatc 720

ggccaggaca tcaactatca ggtcagtcgg caaatctggc aggacatgca atacgacgcg 780

cgctataccc ccggccacct gcagcgttcg ctggtcgatg ccggtctatt gggaaaaaag 840

aacggccgct cctattttgc cgtcgaagaa aacgccccgc cggtgatggc cgccaccgat 900

gcagacattg agacgctgca cgtttacggc gaacaccctt tttttaccct gttacaacaa 960

cgtgccgcgc ttcagtggcc acagctgcgc gtggaacaac ggccggcatt accgggcctg 1020

gggccggccg tccggatcaa tgacgctttc accgtcagca tcaccgatgg ccgcacggca 1080

aaccagctgg ccgagcagac ggcggcggat gcctttgtgg tcgatgtcgc cctgaattac 1140

gccgacacgg cgtatctggt ggcagcgcac agccgccacg cctctgcagc caataaagcg 1200

ctgttcttac gcctgctgca cacggcaatc ccgcaggttg aatttatcaa ggattccccg 1260

gcgctgatcg tcgcccgcgt cctcagcagc ctaatcaacg agtcggtgat catggtggaa 1320

agcggcgtct gcagccggga agacatcgat gtcgccgccg tcgccggcgt taactacgcc 1380

gacggcattt tcggctggct agatagcctg ggggagaaaa acgtcaggac gacgctgagc 1440

aacctggcgc agctgctgca cgcagcacgc tatgcgccgc attacaccct tctgcacgcc 1500

gcgcaaccgg cgctgacgac cacgccttaa 1530

<210> 98

<211> 1530

<212> DNA

<213> Serratia sp. JKS000199

<400> 98

atggcagaaa gtaatgcggc aattcaatcg gctgcgatta tcggcgcggg aacgatgggc 60

agaggcatcg cttatctttt ggcgcaaaaa agcattcgta cggtgcttta taatcgcaac 120

ggcaataccc tcaatcaagc tcgcgacgct atcgtgcaag acctgaacaa aaaggtggaa 180

cagggcaagc tcgcgctgca ggacaaagac gcggtgctgg ctaatctgac gttctccacg 240

gaatttgggg ccatcgccga cagcgatctg gtgatcgaaa ccatcgcaga gcatgagcaa 300

gccaaactcg aagtgctggc ggccatcgcc gcgacggtca agcccgacac gctgatcgcc 360

accaatacct cctcactgtc gcttaataag ctggcgacgg cggtgacgca cagcgaacgc 420

tttatcggtt tgcacttttt caaccccgcg ccgctgatga agctgattga aatcattccg 480

gcctatttta ccgcacaggt caccaccgaa cgttgccgtc aactggtggc ggcgttaggg 540

aaacgcgatg tcgtctgtca ggccacgccg ggatttatcg tcaaccgtat ggcccgcccc 600

tactatctgg aagggttccg cctgttggaa gaacacgtgg cgcgtgcgcc gcagatcgac 660

cgcgccctca aagccggcgg acactttcgc atggggccgc tcgagctgac cgattttatc 720

ggtcaggaca tcaactatca ggtcagccgg caaatttggc aggacatgca gtacgatccg 780

cgctataccc cgggccatct gcagcgctcg ctggtcgatg ccggtctgtt gggcaaaaag 840

aacggccgct cctatttttc cgccgaagaa tctcccccgc cgcttgcggc cgccgtcgat 900

gcggaagtcg agacgctacg catttacggt gaacatcctc tctttaccct gctacagcag 960

cgggccgccc tgcaatggcc gcggctgcgc gtggaacaac ggccgacatt accgggcctg 1020

ggcgccgcca ttcaggtcaa tgacgctttc accgtcagcg ttaccgatgg ccgcacggca 1080

aaccagcttg ccgaacagac cgcggcggac gcctttgtcg tcgatgtcgc cctgaactac 1140

ggcgatacgg cgtacctggt ggcggcgcat agccgccacg cctctgcggc caataaggcg 1200

ctgtttttgc gcctgctgca caccgcgatc ccgctggtgg aatttatcaa ggattccccg 1260

gccttgatcg tcgcccgcgt gctcagcagc ctgatcaacg agtcggtgat catggtggaa 1320

agcggcgtct gcagccggga agacattgat gtcgccgccg tcgccggcgt gaactacgcc 1380

gacggtattt tcggctggct cactcgcctc ggggaggaaa atgtcaggac gacgctgagc 1440

aacctggcgc aattgctgca cgcggcgcgc tatgcgccgc attacaccct tctgcacgcc 1500

gcccaaccgg cgctgaccac cacgccttaa 1530

<210> 99

<211> 1530

<212> DNA

<213> Serratia sp. TEL

<400> 99

atggcagaaa gtaatgcggc aattcaatcg gctgcgatta tcggcgcggg aacgatgggc 60

agaggcatcg cttatctttt ggcgcaaaaa agcattcgta cggtgcttta taatcgcaac 120

ggcaataccc tcaatcaagc tcgcgacgct atcgtgcaag acctgaacaa aaaggtggaa 180

cagggcaagc tcgcgctgca ggacaaagac gcggtgctgg ctaatctgac gttctccgcg 240

gaatttgggg ccatcgccga cagcgatctg gtgatcgaaa ccatcgcaga gcatgagcaa 300

gccaaactcg aggtgctggc ggccatcgcc gcgacggtca agcccgacac gctgatcgcc 360

accaatacct cctcactgtc gcttaataag ctggcgacgg cggtgacgca cagcgaacgc 420

tttatcggtt tgcacttttt caaccccgcg ccgctgatga agctgattga aatcattccg 480

gcctatttta ccgcacaggt caccaccgaa cgttgccgtc aactggtggc ggcgttgggg 540

aaacgcgatg tcgtctgtca ggccacgccg ggatttatcg tcaaccgtat ggcccgcccc 600

tactatctgg aagggttccg cctgttggaa gaacacgtgg cgcgtgcgcc gcagatcgac 660

cgcgccctca aagccggcgg acactttcgc atggggccgc tcgaactgac cgattttatc 720

ggtcaggaca tcaactatca ggtcagccgg caaatttggc aggacatgca gtacgatccg 780

cgctataccc cgggccatct gcagcgctcg ctggtcgatg ccggtctgtt gggcaaaaag 840

aacggccgct cctatttttc cgccgaagaa tctcctccgc cgcttgcggc cgccgtcgat 900

gcggaggtcg agacgctacg catttacggt gaacatcctc tctttactct gctacagcag 960

cgggccaccc tgcaatggcc gcggctgcgc gtggaacaac ggccgacatt accgggcctg 1020

ggcgccgcca ttcaggttaa tgacgctttc accgtcagcg ttaccgatgg ccgcacggca 1080

aaccagcttg ccgaacagac cgcggcggac gcctttgtcg tcgatgtcgc cctgaactac 1140

ggcgatacgg cgtacctggt ggcggcgcat agccgccacg cctctgcagc caataaggcg 1200

ctgtttttac gcctgctgca caccgcgatc ccgcaggtgg aatttatcaa ggattccccg 1260

gccttgatcg tcgcccgcgt gctcagcagc ctgatcaacg agtcggtgat catggtggaa 1320

agcggcgtct gcagccggga agacattgat gtcgccgccg tcgccggcgt gaactacgcc 1380

gacggtattt tcggctggct cactcgcctc ggggaggaaa atgtcagggc gacgctgagc 1440

aacctggcgc aattgctgca cgcggcgcgc tatgcgccgc attacaccct tctgcacgcc 1500

gcccaaccgg cgctgaccac cacgccttaa 1530

<210> 100

<211> 1530

<212> DNA

<213> Serratia sp. ISTD04

<400> 100

atggcagaaa gtaatgcggc aattcaatcg gctgcgatta tcggcgcggg aacgatgggc 60

agaggcatcg cttatctttt ggcgcaaaaa agcattcgta cggtgcttta taatcgcaac 120

ggcaataccc tcaatcaagc tcgcgacgct atcgtgcaag acctgaacaa aaaggtggaa 180

cagggcaagc tcgcgctgca ggacaaagac gcggtgctgg ctaatctgac gttctccgca 240

gaatttgggg tcatcgccga cagcgatctg gtgatcgaaa ccatcgcaga acatgagcaa 300

gccaaactcg aggtgctggc ggccatcgcc gcgacggtca agtccgacac gctgatcgcc 360

accaatacct cctcactgtc gcttaataag ctggcgacgg cggtgacgca cagcgaacgc 420

tttatcggtt tgcacttttt caaccccgcg ccgctgatga agctgattga aatcattccg 480

gcctatttta ccgcacaggt caccaccgaa cgttgccgtc aactggtggc ggcgttaggg 540

aaacgcgatg tcgtctgtca ggccacgccg ggatttatcg tcaaccgtat ggcccgcccc 600

tactatctgg aagggttccg cctgttggaa gaacacgtgg cgcgtgcgcc gcagatcgac 660

cgcgccctca aagccggcgg acactttcgc atggggccgc tcgagctgac cgattttatc 720

ggtcaggaca tcaactatca ggtcagccgg caaatttggc aggacatgca gtacgatccg 780

cgctataccc cgggccatct gcagcgctcg ctggtcgatg ccggtctgtt gggcaaaaag 840

aacggccgct cctatttttc cgccgaagaa tctcccccgc cgcttgcggc cgccgtcgat 900

gcggaagtcg agacgctacg catttacggt gaacatcctc tctttactct gctacagcag 960

cgggccgccc tgcaatggcc gcggctgcgc gtggaacaac ggccgacatt accgggcctg 1020

ggcgccgcca ttcaggtcaa tgacgctttc accgtcagcg ttaccgatgg ccgcacggca 1080

aaccagcttg ccgaacagac cgcggcggac gcctttgtcg tcgatgtcgc cctgaactac 1140

ggcgatacgg cgtacctggt ggcggcacat agccgccatg cctctgcggc caataaggcg 1200

ctgtttttac gcctgctgca caccgcgatc ccgcaggtgg aatttatcaa ggattccccg 1260

gccttgatcg tcgcccgcgt gctcagcagc ctgatcaacg agtcggtgat catggtggaa 1320

agcggcgtct gcagccggga agacattgat gtcgccgccg tcgccggcgt gaactacgcc 1380

gacggtattt tcggctggct cactcgcctc ggggaggaaa atgtcaggac gacgctgagc 1440

aacctggcgc aattgctgca cgcggcgcgc tatgcgccgc attacaccct tctgcacgcc 1500

gcccaaccgg cgctgaccac cacgccttaa 1530

<210> 101

<211> 1530

<212> DNA

<213> Serratia sp. SCBI

<400> 101

atggcagaaa gtaatgcggc aattcaatcg gctgcgatta tcggcgcggg aacgatgggc 60

agaggcatcg cttatctttt ggcgcaaaaa agcattcgta cggtgcttta taatcgcaac 120

ggcaataccc tcaatcaagc tcgcgacgct atcgtgcaag acctgaacaa aaaggtggaa 180

cagggcaagc tcgcgctgca ggacaaagac gcggtgctgg ctaatctgac gttctccgcg 240

gaatttgggg ccatcgccga cagcgatctg gtgatcgaaa ccatcgcaga gcatgagcaa 300

gccaaactcg aggtgctggc ggccatcgcc gcgacggtca agcccgacac gctgatcgcc 360

accaatacct cctcactgtc gcttaataag ctggcgacgg cggtgacgca cagcgaacgc 420

tttatcggtt tgcacttttt caaccccgcg ccgctgatga agctgattga aatcattccg 480

gcctatttta ccgcacaggt taccaccgaa cgttgccgtc aactggtggc ggcgttgggg 540

aaacgcgatg tcgtctgtca ggccacgccg ggatttatcg tcaaccgtat ggcccgcccc 600

tactatctgg aagggttccg cctgttggaa gaacacgtgg cgcgtgcgcc gcagatcgac 660

cgcgccctca aagccggcgg acactttcgc atggggccgc tcgagctgac cgattttatc 720

ggtcaggaca tcaactatca ggtcagccgg caaatttggc aggacatgca gtacgatccg 780

cgctataccc cgggccatct gcagcgctcg ctggtcgatg ccggtctgtt gggcaaaaag 840

aacggccgct cctatttttc caccgaagaa tctcccccgc cgcttgcggc cgccgtcgat 900

gcggaggtcg agacgctacg catttacggt gaacatcctc tctttaccct gctacagcag 960

cgggccgccc tgcaatggcc gcggctgcgc gtggaacaac ggccgacatt accgggcctg 1020

ggcgccgcca ttcaggtcaa tgacgctttc accgtcagcg ttaccgatgg ccgcacggca 1080

aaccagcttg ccgaacagac cgcggcggac gcctttgtcg tcgatgtcgc cctgaactac 1140

ggcgatacgg cgtacctggt ggcggcgcat agccgccacg cctctgcggc caataaggcg 1200

ctgtttttac gcctgctgca caccgcgatc ccgcaggtgg aatttatcaa ggattccccg 1260

gccttgatcg tcgcccgcgt gctcagcagc ctgatcaacg agtcggtgat catggtggaa 1320

agcggcgtct gcagccggga agacattgat gtcgccgccg tcgccggcgt gaactacgcc 1380

gacggcattt tcggctggct cactcgcctc ggggaggaaa atgtcaggac gacgctgagc 1440

aacctggcgc aattgctgca cgcggcgcgc tatgcgccgc attacaccct tctgcacgcc 1500

gcccaaccgg cgctgaccac cacgccttaa 1530

<210> 102

<211> 1530

<212> DNA

<213> Serratia sp. S4

<400> 102

atggcagaga ataattcggc aatccattcg gtcgctgtta ttggtgccgg gaccatgggc 60

agaggtattg cctatcttct ggcgcaaaac ggcatacgaa ccctgcttta taatcgtagc 120

ggtaataatc tgaatcaggc cagggactat attatccgtg acctggataa gaaaatagat 180

ggcggtaaaa taagcctgca gaagaaaggc gagatattgg ccaatctggt tttctctcct 240

attttcgagg ctattgccga cagcgacctg gtgattgaaa ccatcgcgga acatgagtca 300

accaagcatg agatcctctc ggcgattgcg gccacggtga aaaaagaggc gattatcgcc 360

accaatacct catcgctgtc attgaataag ctggcggcag gcgtcgaaaa taacgcccgt 420

tttatcggcc tgcacttctt caatccggcc ccgctgatga aactgatcga aattattccg 480

tcttacttta ccagccgggc caccagcctg cgttgccagc agttggtgac ggtgataggc 540

aaacagtttg tggtctgcaa agccacgccg ggctttatcg tcaaccggat ggcgcgacct 600

ttttatctgg aagggtttcg gctgttggaa gagaacgtgg cgctggcgcc gcagatcgac 660

cgcgcactca aggccggtgg ccacttccgc atgggacctt tagagctgac cgattttatc 720

ggccaggata ttaactatca ggtcagcagc cagatttggc aggacatgca gtacgacccc 780

cgctataccc ccggtcattt gcaacgttcg ctggtggatg ccgggctgct ggggaagaaa 840

aacggccgat ccttttttac cccctcttcc gccacaccca gctccgccga cgcaggcagc 900

ggcacgccga cctcactgaa tttttatggt gaacatcccc tgttcgacct gttgcaacag 960

cgcgctttgg cgctctggcc aaggttgcag attaatcgcc aatcggaaca gccaacgctg 1020

ggccgcttta tccgggtgaa tgacgcaatg gccatcaaaa ttaccgatgg ccgcaccgcc 1080

aatctgctgg ctgaattgac cgaactcgat accttcgtga tcgacgccgc gctcaactac 1140

gccgataccg cctatctggc ggctgcccac agccaggatg ccagcacggc caataaagcg 1200

ctgtttctga cgctgctgca aacgttgatc ccacaggtgg agtttattaa agactccccg 1260

ggcctgattg tcgcccgcgt cctgagcagc ctgatcaatg agtcggtgat tatggtggag 1320

agcggggttt gcagccgggc ggacatcgat atcgccgcgg tggccggcgt taactatgcc 1380

gatggcatct ttagctggct ggcgcagctt gggcaaaaaa acgtgaagtc gacgctggac 1440

aatatggctc aactgctgca ttccgcccgc tattacccgc attactcttt gctcaatacc 1500

ccccggccag agctggccgt cgcgccctaa 1530

<210> 103

<211> 1530

<212> DNA

<213> Serratia sp. C-1

<400> 103

atggcagaga ataattcggc aatccgttca gccgccgtta ttggtgcggg gaccatgggc 60

agaggcatcg cctacctcct ggcactgaac ggcatacgaa ccgtacttta taatcgcaat 120

ggtaataatc tcaatcaggc ccgtgactat attgtcagcg acctggacag aaaaatagat 180

aacggaaaaa taaccctgca gaaaaaaggc cagatattag ccaatattat tttctcggcc 240

gtctttgacg ccataaccga cagcgatctg gtgattgaaa ccattgcgga agatgagcaa 300

accaagcatg aaatcctggc agccattgcg gctacggtaa aaccggaggc gatcattgcg 360

accaatacct cctcgttgtc gctgaacaaa ctggcagcag gggtggaaaa caacccgcgc 420

tttatcggcc tgcatttttt caatccggcg ccgctgatga agttgatcga aatcattccc 480

tcttatttta cctcccacgc caccagccta cgctgccaga agttggtaat agcattgggt 540

aaacagtttg tggtctgcaa agccacgccg ggctttattg ttaatcgcat ggcgcggcct 600

ttctatctgg aagggttccg gctgctggag gaaaacgtgg cgctggcgcc acagatcgac 660

cgcgccctca aggccggcgg gcattttcgc atgggccctt tagaactgac ggattttatc 720

ggtcaggata tcaactacca ggtcagcaag cagatttggc aggatatgca gttcgaccct 780

cgctataccc ctggtcattt gcaacgctcg ctggtggatg ccgggctgct ggggaggaaa 840

aacgggcgct ctttttttgc ttcccaaccg gcgacgccgc ccaccccgac cacagagagc 900

gacacgccaa cgtcactgca tttttatggg gagcatgctt tattcgatca tctgcaacag 960

cgtgctctgg ccgcctggcc tgcgctgcgc gttcagcggt tgccggaacg gcctgaactg 1020

gggcgattta tcctggtgaa taacgcgctg gcgatcaaaa tcaccgatgg cagaacggca 1080

aacctgctcg ccggcttaac cgctctcgac accttcgtga ttgacgctgc gctgaattac 1140

gccgacaccg cctatctggt ggcagcccac aatcaacatg ccacagagac gaataaagcg 1200

ctgtttctga cgctgctgca aaccgtcatc gctcaggtgg agtttattaa agattcccct 1260

gccctgatcg ttgcccgcgt actgagcagc ctgatcaatg aatcggtgat catggtggag 1320

agcggcgttt gcagccgggc agatatcgat atcgccgccg tggccggcgt gaactatgcc 1380

gacggcattt ttgcctggtt ggcgcagctc gggcagaaaa acgtgaaatc gacgctggat 1440

aacatggcgc aactgctgca ctccgcgcgc tattacccgc attactcttt gctgaacgcg 1500

gcccggcctg agctggccgt agcgccctaa 1530

<210> 104

<211> 1530

<212> DNA

<213> Serratia marcescens 532

<400> 104

atggcagaaa gtaatgcggc aattcaatcg gctgcgatta tcggcgcggg aacgatgggc 60

agaggcatcg cttatctttt cgcgcaaaaa ggcattcgca cggtgcttta taatcgcaac 120

ggcaataccc tcaatcaggc tcgcgaatat atcgcgcaag acctgaacaa gaaagtcgaa 180

cagggcaaga tcgcgctgca ggataaaggc gcggtgctgg ccaatctgat gttcacttca 240

gtgtttgagg ccatcaccga cagcgagctg gtgatagaaa ccatcgccga gcaagaacaa 300

accaaacttg aggtgctggc ggccatcgcc gcggtggtca agcccgacac gctgatcgcc 360

accaatacct cctcactgtc gcttaacaag ctggctactg cggtgacgca cagcgaacgc 420

tttatcggtt tgcatttttt caaccccgcg ccgctgatga agctgattga aatcattccg 480

gcctacttta ccgcgcacgc caccaccgaa cgctgccgcc aactggtggc ggcgttgggg 540

aaacacgatg tcgtctgcca ggccacgccg gggttcatcg tcaatcgcat ggcccgcccc 600

tactacctgg aagggttccg tctgttggaa gaacacgtgg cgcgcgcggc gcagatcgac 660

cgcgctctca aggccggcgg gcgcttccgc atggggccgc tcgagctgac cgattttatc 720

ggccaagaca tcaactatca ggtcagtcgg caaatctggc aggacatgca atacgacccg 780

cgctataccc ccggtcatct gcagcgttca ctggtcgatg ccggtctgtt ggggaaaaag 840

aacggccgct cctattttgc cgccgaagaa accgccccgc cggtgacggc cgccagcaat 900

gcagacgtcg agacgctgcg cgtttacggc gagcaccctt tttttaccct gttacagcag 960

cgagccgcgc ttcagtggcc acagctgcgc gtggaacaac ggccggcatt accggggctg 1020

gggtcggccg tccagatcaa tgacgctttc accgtcagca tcaccgatgg ccgcacggcg 1080

agccaactgg ccgagcagac ggcagcagat gcctttgtgg tcgatgtcgc cctgaactac 1140

gccgacacga cgtatctggc ggcggcgcac agccgccacg cctctgcggc caataaggcg 1200

ctgtttttac gcctgctgca cacggcaatc ccgcaggttg aatttatcaa ggactctccg 1260

gcgcttatcg tcgcccgcgt cctcagcagc ctgatcaatg agtcggtgat catggtggaa 1320

agcggcgtct gcagccggga agacatcgat gtcgccgccg tcgcgggcgt taactacgcc 1380

ggcggcattt tcgactggct cggcaaactg ggggagaaaa acgtcaggac aacactgagc 1440

aatctggctc agctgctgca cgcggcgcgc tatgcgccgc attacaccct tctgcacgcc 1500

gcgcaaccgg cgctgacgac cacgccttaa 1530

<210> 105

<211> 1530

<212> DNA

<213> Serratia marcescens 2880STDY5683033

<400> 105

atggcagaaa gtaatgcggc aattcaatcg gctgcgatta tcggcgcggg aacgatgggc 60

agaggcatcg cttatctttt cgcgcaaaaa ggcattcgca cggtgcttta taatcgcaac 120

ggcaataccc tcaatcaggc tcgcgaatat atcgcgcacg acctgaacaa gaaagtcgaa 180

caaggcaaga tcgcgctgca ggataaaggc gcggtgctgg ccaatctaat gttcacttca 240

gtgtttgagg ccatcgccga cagcgagctg gtgatagaaa ccatcgccga gcaagaacaa 300

accaaacttg aggtgctggc ggccatcgcc gcggtggtca agcccgacac gctgatcgcc 360

accaatacct cctcactgtc gcttaacaag ctggctactg cggtgacgca cagcgaacgc 420

tttatcggtt tgcatttttt caaccccgcg ccgctgatga agctgattga aatcattccg 480

gcctacttta ccgcgcacgc caccaccgaa cgctgccgcc aactggtagc ggcgttgggg 540

aaacacgatg tcgtctgcca ggccacgccg gggttcatcg tcaatcgcat ggcccgcccc 600

tactacctgg aagggttccg cctgttggaa gaacacgtgg cgcgcgcggc gcagatcgac 660

cgcgccctca aggccggcgg gcgcttccgc atggggccgc tcgagctgac cgattttatc 720

ggccaagaca tcaactatca ggtcagtcgg caaatctggc aggatatgca atacgacccg 780

cgctataccc ccggtcatct gcagcgttca ctggtcgatg ccggtctgtt ggggaaaaag 840

aacggccgct cctattttgc caccgaagaa accgccccgc cggtgacggc cgccagcaat 900

gcagacgtcg agacgctgcg cgtttacggc gagcaccctt tttttaccct gttacagcag 960

cgagccgcgc ttcagtggcc acagctgcgc gtggaacaac ggccggcatt accggggctg 1020

gggtcggccg tccagatcaa tgacgctttc accgtcagca tcaccgatgg ccgcacggcg 1080

agccaactgg ccgagcagac ggcagcggat gcctttgtgg tcgatgtcgc cctgaactac 1140

gccgacacga cgtatctggc ggcggcgcac agccgccacg cctctgcggc caataaggcg 1200

ctgtttttac gcctgctgca cacggcaatc ccgcaggttg aatttatcaa ggactctccg 1260

gcgcttatcg tcgcccgcgt cctcagcagc ctgatcaatg agtcggtgat catggtggaa 1320

agcggcgtct gcagccggga agacatcgat gtcgccgccg tcgcgggcgt taactacgcc 1380

ggcggcattt tcgactggct cggcaaactg ggggagaaaa acgtcaggac aacgctgagc 1440

aatctggctc agctgctgca cgcggcgcgc tatgcgccgc attacaccct tctgcacgcc 1500

gcgcaaccgg cgctgacgac cacgccttaa 1530

<210> 106

<211> 1530

<212> DNA

<213> Serratia marcescens WW4

<400> 106

atggcagaaa gtaatgcggc aattcaatcg gctgcgatta tcggcgcggg aacgatgggc 60

agaggcatcg cttatctttt cgcgcaaaaa ggcattccca cgatgcttta taatcgcaac 120

ggcaataccc tcaatcaagc tcgcgaatat atcgcgcaag acctgaacaa gaaagtcgaa 180

cagggcaaga tcgcgctgca ggataaagac gcggtgctgg ccaatctgat gttcacttcc 240

gtgtttgagg ccatcgccga cagcgagctg gtgatagaaa ccatcgccga gcaagaacaa 300

accaaacttg aggtgctggc ggccatcgcc gcagtggtca agcccgacac gctgatcgcc 360

accaatacct cctcactgtc gcttaacaag ctggcaactg cggtgacgca cagcgagcgc 420

tttatcggtt tgcatttttt caaccccgcg ccgctgatga agctgattga aatcattccg 480

gcctacttta ccgcacacgc caccacggaa cgctgccgcc aactggtggc ggcgttgggg 540

aaacgcgatg tcgtctgcca ggccacgccg gggttcatcg tcaatcgcat ggcccgcccc 600

tactacctgg aagggttccg cctgttggaa gaacatgtgg cgcgcgcggc gcagatcgac 660

cgcgccctca aggccggcgg gcgcttccgc atggggccgc tcgagctgac cgattttatc 720

ggccaagaca ttaactatca ggtcagccgg caaatctggc aggacatgca atacgacccg 780

cgctataccc ctggtcatct gcagcgttca ctggtcgatg ccggtctgtt ggggaaaaag 840

aacggccgct cctattttgc cgccgaagaa accgccccgc cggtgacggc cgccaacaat 900

gcagacgtcg agacgctgcg cgtttacggc gagcatcctt tttttaccct gttgcagcag 960

cgagccgcgc ttcagtggcc acagctgcgc gtggaacaac ggccggcatt accggggctg 1020

ggagcggccg tccagatcaa tgacgctttc accgtcagca tcaccgatgg ccgcacggcg 1080

agccaactgg ccgagcagac ggcagcggat gcctttgtga tcgatgtcgc cctgaactac 1140

gccgacacca cgtatctggt ggcggcgcac agccgccacg cttctgcggc caataaggcg 1200

ctgtttttac gcctgctgca cacggcaatc ccgcaggttg aatttatcaa ggactccccg 1260

gcgcttatcg tcgcccgcgt cctcagcagc ctgatcaatg agtcggtgat catggtggaa 1320

agcggcgtct gcagccggga agacatcgat gtcgccgccg tcgcgggcgt taactacgcc 1380

ggcggcattt tcgactggct cggcaaactg ggggagaaaa acgtcaggac gacgctgagc 1440

aatctggcgc agctgctgca cgcggcgcgc tatgcgccgc attacaccct tctgcacgcc 1500

gcgcaaccgg cgctgacgac cacgccttaa 1530

<210> 107

<211> 1530

<212> DNA

<213> Serratia marcescens K27

<400> 107

atggcagaaa gtaatgcggc aattcaatcg gctgcgatta tcggcgcggg aacgatgggc 60

agaggcatcg cttatctttt cgcgcaaaaa ggcattccca cgatgcttta taatcgcaac 120

ggcaataccc tcaatcaagc tcgcgaatat atcgcgcaag acctgaataa gaaagtcgaa 180

cagggcaaga tcgcgctgca ggataaagac gcggtgctgg ccaatctgat gttcacttcc 240

gtgtttgagg ccatcgccga cagcgagctg gtaatagaaa ccatcgccga gcaagaacaa 300

accaaacttg aggtgctggc ggccatcgcc gcggtggtca agcccgacac gctgatcgcc 360

accaatacct cctcactgtc gctcaacaag ctggcaactg cggtgacgca cagcgaacgc 420

tttatcggtt tacatttttt caaccccgcg ccgctgatga agctgattga aatcattccg 480

gcctacttta ccgcgcacgc caccacggaa cgctgccgcc aactggtggc ggcgttaggg 540

aaacgcgatg tcgtctgcca ggccacgccg gggttcatcg tcaatcgcat ggcccgcccc 600

tactacctgg aagggttccg cctgttggaa gaacatgtgg cgcgctcggc gcagatcgac 660

cgcgccctca aggccggcgg gcgcttccgc atggggccgc tcgagctgac cgattttatc 720

ggccaagaca ttaactatca ggtcagccgg caaatctggc aggacatgca atacgacccg 780

cgctataccc ctggtcatct gcagcgttca ctggtcgatg ccggtctgtt ggggaaaaag 840

aacggccgct cctattttgc cgccgaagaa accgccccgc cggtgacggc cgccaacaat 900

gcagacgtcg agacgctgcg cgtttacggc gagcatcctt tttttaccct gttgcagcag 960

cgagccgcgc ttcagtggcc acagctgcgc gtggaacaac ggccggcatt accggggctg 1020

ggagcggccg tccagatcaa tgacgctttc accgtcagca tcaccgatgg ccgcacggcg 1080

agccaactgg ccgagcagac ggcagcggat gcctttgtgg tcgatgtcgc cctgaactac 1140

gccgacacca cgtatctggt ggcggcgcac agccgccacg cttctgcggc caataaggcg 1200

ctgtttttac gcctgctgca cacggcaatc ccgcaggttg aatttatcaa ggactctccg 1260

gcgcttatcg tcgcccgcgt cctcagcagc ctgatcaatg agtcggtgat catggtggaa 1320

agcggcgtct gcagccggga agacatcgat gtcgccgccg tcgcgggcgt taactacgcc 1380

ggcggcattt tcgactggct cggcaaactg ggggagagaa acgtcaggac gacgctgagc 1440

aatctggcgc agctgctgca cgcggcgcgc tatgcgccgc attacaccct tctgcacgcc 1500

gcgcaaccgg cgctgacgac cacgccttaa 1530

<210> 108

<211> 1530

<212> DNA

<213> Serratia marcescens 280

<400> 108

atggcagaaa gtaatgcggc aattcaatcg gctgcgatta tcggcgcggg aacgatgggc 60

agaggcatcg cttatctttt cgcgcaaaaa ggcattccca cgatgcttta taatcgcaac 120

ggcaataccc tcaatcaagc tcgcgaatat atcgcgcaag acctgaataa gaaagtcgaa 180

cagggcaaga tcgcgctgca ggataaagac gcggtgctgg ccaatctgat gttcacttcc 240

gtgtttgagg ccatcgccga cagcgagctg gtaatagaaa ccatcgccga gcaagaacaa 300

accaaacttg aggtgctggc ggccatcgcc gcagtggtca agcccgacac gctgatcgcc 360

accaatacct cctcactgtc gcttaacaag ctggcaactg cggtaacgca cagcgaacgc 420

tttatcggtt tgcatttttt caaccccgcg ccgctgatga agctgattga aatcattccg 480

gcctacttta ccgcgcacgc caccacggaa cgctgccgcc aactggtggc ggcgttgggg 540

aaacgcgatg tcgtctgcca ggccacgccg gggttcatcg tcaatcgcat ggcccgcccc 600

tactacctgg aagggttccg cctgttggaa gaacatgtgg cgcgcgcggc gcagatcgac 660

cgcgccctca aggctggcgg gcgcttccgc atggggccgc tcgagctgac cgattttatc 720

ggccaagaca tcaactatca ggtcagccgg caaatctggc aggacatgca atacgacccg 780

cgctataccc ctggtcatct gcagcgttca ctggtcgatg ccggtctgtt ggggaaaaag 840

aacggccgct cctattttgc cgccgaagaa accgccccgc cggtgacggc cgccaacaat 900

gcagacgtcg agacgctgcg cgtttacggc gagcaccctt tttttaccct gttgcagcag 960

cgagccgcgc ttcagtggcc acagctgcgc gtggaacaac ggccggcatt accggggctg 1020

ggagcggccg tccagatcaa tgacgctttc accgtcagca tcaccgatgg ccgcacggcg 1080

agccaactgg ccgagcagac ggcagcggat gcctttgtgg tcgatgtcgc cctgaactac 1140

gccgacacca cgtatctggt ggcggcgcac agccgccacg cttctgctgc caataaggcg 1200

ctgtttttac tcctgctgca cacggcaatc ccgcaggttg aatttatcaa ggactccccg 1260

gcgcttatcg tcgcccgcgt cctcagcagc ctgatcaatg agtcggtgat catggtggaa 1320

agcggcgtct gcagccggga agacatcgat gtcgccgccg tcgcgggcgt taactacgcc 1380

ggcggcattt tcgactggct cggcaagctg ggggagaaaa acgtcaggac gacgctgagc 1440

aatctggcgc agctgctgca cgcggcgcgc tatgcgccgc attacaccct tctgcacgcc 1500

gcgcaaccgg cgctgacgac cacgccttaa 1530

<210> 109

<211> 1530

<212> DNA

<213> Serratia marcescens 19F

<400> 109

atggcagaaa gtaatgcggc aattcaatcg gctgcgatta tcggcgcggg aacgatgggc 60

agaggcatcg cttatctttt cgcgcaaaaa ggcattccca cgatgcttta taatcgcaac 120

ggcaataccc tcaatcaagc tcgcgaatat atcgcgcaag acctgaacaa gaaagtcgaa 180

cagggcaaga tcgcgctgca ggataaggac gcggtgctgg ccaatctgat gttcacttcc 240

gtgtttgagg ccatcgccga cagcgagctg gtaatagaaa ccatcgccga gcaagaacaa 300

accaaacttg aggtgctggc ggccatcgcc gcggtggtca agcacgacac gctgatcgcc 360

accaatacct cctcactgtc gcttaacaag ctggcaactg cggtgacgca cagcgaacgc 420

tttatcggtt tgcatttttt caaccccgcg ccgctgatga agctgattga aatcattccg 480

gcctacttta ccgcgcacgc caccacggaa cgctgccgcc aactggtggc ggcgttgggg 540

aaacgcgatg tcgtctgcca ggccacgccg gggttcatcg tcaatcgcat ggcccgcccc 600

tactacctgg aagggttccg cctgttggaa gaacatgtgg cgcgcgcggc gcagatcgac 660

cgcgccctca aggccggcgg gcgcttccgc atggggccgc tcgagctgac cgattttatc 720

ggccaagaca ttaactatca ggtcagccgg caaatctggc aggacatgca atacgacccg 780

cgctataccc ctggtcatct gcagcgttca ctggtcgatg ccggtctgtt ggggaaaaag 840

aacggccgct cctattttgc cgccgaagaa accgccccgc cggtgacggc cgccaacaat 900

gcagacgtcg agacgctgcg cgtttacggc gagcatcctt tttttaccct gttgcagcag 960

cgagccgcgc ttcagtggcc acagctgcgc gtggaacaac ggccggcatt accggggctg 1020

ggagcggccg tccagatcaa tgacgctttc accgtcagca tcaccgatgg ccgcacggcg 1080

agccaactgg ccgagcagac ggcagcggat gcctttgtgg tcgatgtcgc cctgaactac 1140

gccgacacca cgtatctggt ggcggcgcac agccgccacg cttctgcggc caataaggcg 1200

ctgtttttac gcctgctgca cacggcaatc ccgcaggttg aatttatcaa ggactccccg 1260

gcgcttatcg tcgcccgcgt cctcagcagc ctgatcaatg agtcggtgat catggtggaa 1320

agcggcgtct gcagccggga agacatcgat gtcgccgccg tcgcgggcgt taactacgcc 1380

ggcggcattt tcgactggct cggcaaactg ggggagaaaa acgtcaggac gacgctgagc 1440

aatctggcgc agctgctgca cgcggcgcgc tatgcgccgc attacaccct tctgcacgcc 1500

gcgcaaccgg cgctgacgac cacgccttaa 1530

<210> 110

<211> 1530

<212> DNA

<213> Serratia marcescens 1185

<400> 110

atggcagaaa gtaatgcggc aattcaatcg gctgcgatta tcggcgcggg aacgatgggc 60

agaggcatcg cttatctttt cgcgcaaaaa ggcattccca cgatgcttta taatcgcaac 120

ggcaataccc tcaatcaagc tcgcgaatat atcgcgcaag acctgaacaa gaaagtcgaa 180

cagggcaaga tcgcgctgcg ggataaagac gcggtgctgg ccaatctgat gttcacttcc 240

gtgtttgagg ccatcgccga cagcgagctg gtgatagaaa ccatcgccga gcaagaacaa 300

accaaacttg aggtgctggc ggccatcgcc gcggtggtca agcccgacac gctgatcgcc 360

accaatacct cctcactgtc gcttaacaag ctggctactg cggtgacgca cagcgaacgc 420

tttatcggtt tgcatttttt caaccccgcg ccgctgatga agctgattga aatcattccg 480

gcctacttta ccgcgcacgc caccaccgaa cgctgccgcc aactggtggc ggcgttgggg 540

aaacacgatg tcgtctgcca ggccacgccg gggttcatcg tcaatcgcat ggcccgcccc 600

tactacctgg aagggttccg cctgttggaa gaacatgtgg cgcgcgcggc gcagatcgac 660

cgcgccctca aggccggcgg gcgcttccgc atggggccgc tcgagctgac cgattttatc 720

ggccaagaca tcaactatca ggtcagccgg caaatctggc aggacatgca atacgacccg 780

cgctataccc ctggtcatct gcagcgttca ctggtcgatg ccggtctgtt ggggaaaaag 840

aacggccgct cttattttgc cgccgaagaa accgccccgc cggtgacggc cgccaacaat 900

gcagacgtcg agacgctgcg cgtttacggc gagcatcctt tttttaccct gttgcagcag 960

cgagccgcgc ttcagtggcc acagctgcgc gtggaacaac ggccggcatt accggggctg 1020

ggagcggccg tccagatcaa tgacgctttc accgtcagca tcaccgatgg ccgcacggcg 1080

agccaactgg ccgagcagac ggcagcggat gcctttgtgg tcgatctcgc cctgaactac 1140

gccgacacca cgtatctggt ggcggcgcac agccgccacg cttctgcggc caataaggcg 1200

ctgtttttac gcctgctgca cacggcaatc ccgcaggttg aatttatcaa ggactctccg 1260

gcgcttatcg tggcccgcgt cctcagcagc ctgatcaatg agtcggtgat catggtggaa 1320

agcggcgtct gcagccggga agacatcgat gtcgccgccg tcgcgggcgt taactacgcc 1380

ggcggcattt tcgactggct cggcaaactg ggggagaaaa acgtcaggac gacgctgagc 1440

aatctggcgc agctgctgca cgcggcgcgc tatgcgccgc attacaccct tctgcacgcc 1500

gcgcaatcgg cgctgacgac cacgccttaa 1530

<210> 111

<211> 1530

<212> DNA

<213> Serratia marcescens S2I7

<400> 111

atggcagaaa gtaatgcggc aattcaatcg gctgcgatta tcggcgcggg aacgatgggc 60

agaggcatcg cttatctttt cgcgcaaaaa ggcattccca cgatgcttta taatcgcaac 120

ggcaataccc tcaatcaagc tcgcgaatat atcgcgcaag atctgaataa gaaagtcgaa 180

cagggcaaga tcgcgctgca ggataaggac gcggtgctgg ccaatctgat gttcacttcc 240

gtgtttgagg ccatcgccga cagcgagctg gtgatagaaa ccatcgccga gcaagaacaa 300

accaaacttg aggtgctggc ggccatcgcc gcggtggtca agcccgacac gctgatcgcc 360

accaatacct cctcactgtc gcttaacaag ctggcaactg cggtgacgca cagcgaacgc 420

tttatcggtt tgcatttttt caaccccgcg ccgctgatga agctgattga aatcattccg 480

gcctacttta ccgcgcacgc caccacggaa cgctgccgcc aactggtggc ggcgttgggg 540

aaacgcgatg tcgtctgcca ggccacgccg gggttcatcg tcaatcgcat ggcccgcccc 600

tactacctgg aagggttccg cctgttggaa gaacatgtgg cgcgcgcggc gcagatcgac 660

cgcgccctca aggccggcgg gcgcttccgc atggggccgc tcgagctgac cgattttatc 720

ggccaagaca tcaactatca ggtcagccgg caaatctggc aggacatgca atacgacccg 780

cgctataccc ctggtcatct gcagcgttca ctggtcgatg ccggtctgtt ggggaaaaag 840

aacggccgct cctattttgc cgccgaagaa accgccccgc cggtgacggc cgccaacaat 900

gcagacgtcg agacgctgcg cgtttacggc gagcatcctt tttttaccct gttgcagcag 960

cgagccgcgc ttcagtggcc acagctgtgc gtggaacaac ggccggcatt accggggctg 1020

ggagcggccg tccagatcaa tgacgctttc accgtcagca tcaccgatgg ccgcacggcg 1080

agccaactgg ccgagcagac agcagcggat gcctttgtgg tcgatgtcgc cctgaactac 1140

gccgacacca cgtatctggt ggcagcgcac agccgccacg cttctgcggc caataaggcg 1200

ctgtttttac gcctgctgca cacggcaatc ccgcaggttg aatttatcaa ggactctccg 1260

gcgcttatcg tcgcccgcgt cctcagcagc ctgatcaatg agtcggtgat catggtggaa 1320

agcggcgtct gcagccggga agacatcgat gtcgccgccg tcgcgggcgt taactacgcc 1380

ggcggcattt tcgactggct cggcaaactg ggggagagaa acgtcaggac gacgctgagc 1440

aatctggcgc agctgctgca cgcggcgcgc tatgcgccgc attacaccct tctgcacgcc 1500

gcgcaaccgg cgctgacgac cacgccttaa 1530

<210> 112

<211> 1530

<212> DNA

<213> Serratia marcescens KHCo-24B

<400> 112

atggcagaaa gtaatgcggc aattcaatcg gctgcgatta tcggcgcggg aacgatgggc 60

agaggcatcg cttatctttt cgcgcaaaaa ggcattccca cgatgcttta taatcgcaac 120

ggcaataccc tcaatcaagc tcgcgaatat atcgcgcaag acctgaataa gaaagtcgaa 180

cagggcaaga tcgcgctgcg ggataaagac gcggtgctgg ccaatctgat gttcacttcc 240

gtgtttgagg ccatcgccga cagcgagctg gtgatagaaa ccatcgccga gcaagaacaa 300

accaaacttg aggtgctggc ggccatcgcc gcagtggtca agcccgacac gctgatcgcc 360

accaatacct cctcactgtc gctcaacaag ctggcaacgg cggtaacgca cagcgaacgc 420

tttatcggtt tgcatttttt caaccccgcg ccgctgatga agctgattga aatcattccg 480

gcctacttta ccgcgcacgc caccacggaa cgctgccgcc aactggtggc ggcgttgggg 540

aaacgcgatg tcgtctgcca ggccacgccg gggttcatcg tcaatcgcat ggcccgcccc 600

tactacctgg aagggttccg cctgttggaa gaacatgtgg cgcgcgcggc gcagatcgac 660

cgcgccctca aggctggcgg gcgcttccgc atggggccgc tcgagctgac cgattttatc 720

ggccaagaca tcaactatca ggtcagccgg caaatctggc aggacatgca atacgacccg 780

cgctataccc ctggtcatct gcagcgttca ctggtcgatg ccggtctgtt ggggaaaaag 840

aacggccgct cctattttgc cgccgaagaa accgccccgc cggtgacggc cgccaacaat 900

gcagacgtcg agacgctgcg cgtttacggc gagcatcctt tttttaccct gttgcagcag 960

cgagccgcgc ttcagtggcc acagctgcgc gtggaacaac ggccggcatt accggggctg 1020

ggagcggccg tccagatcaa tgacgctttc accgtcagca tcaccgatgg ccgcacggcg 1080

agccaactgg ccgagcaggc ggcagcggat gcctttgtgg tcgatctcgc cctgaactac 1140

gccgacacca cgtatctggt ggcggcgcac agccgccacg cttctgcggc caataaggcg 1200

ctgtttttac gcctgctgca cacggcaatc ccgcaggttg aatttatcaa ggactctccg 1260

gcgcttatcg tcgcccgcgt cctcagcagc ctgatcaatg agtcggtgat catggtggaa 1320

agcggcgtct gcagccggga agacatcgat gtcgccgccg tcgcgggcgt taactacgcc 1380

ggcggcattt tcgactggct cggcaaactg ggggagaaaa acgtcaggac gacgctgagc 1440

aatctggcgc agctgctgca cgcggcgcgc tatgcgccgc attacaccct tctgcacgcc 1500

gcgcaaccgg cgctgacgac cacgccttaa 1530

<210> 113

<211> 1530

<212> DNA

<213> Serratia marcescens Z6

<400> 113

atggcagaaa gtaatgcggc aattcaatcg gctgcgatta ttggcgcggg aacgatgggc 60

agaggcatcg cttatctttt cgcgcaaaaa ggcattccca cgatgcttta taatcgcaac 120

ggcaataccc tcaatcaagc tcgcgaatat atcgcgcaag acctgaacaa gaaagtcgaa 180

cagggcaaga tcgcgctgca ggataaagac gcggtgctgg ccaatctgat gttcacttcc 240

gtgtttgagg ccatcgccga cagcgagctg gtgatagaaa ccatcgccga gcaagaacaa 300

accaaacttg aggtgctggc ggccatcgcc gcggtggtca agcccgacac gctgatcgcc 360

accaatacct cctcactgtc gcttaacaag ctggcaactg cggtgacgca cagagagcgc 420

tttatcggtt tgcatttttt caaccccgcg ccgctgatga agctgattga aatcattccg 480

gcctacttta ccgcgcacgc caccacggaa cgctgccgcc aactggtggc ggcgttgggg 540

aaacgcgatg tcgtctgcca ggccacgccg gggttcatcg tcaatcgcat ggcccgcccc 600

tactacctgg aagggttccg cctgttggaa gaacatgtgg cgcgcgcggc gcagatcgac 660

cgcgccctca aggccggcgg gcgcttccgc atggggccgc tcgagctgac cgattttatc 720

ggccaagaca ttaactatca ggtcagccgg caaatctggc aggacatgca atacgacccg 780

cgctataccc ctggtcattt gcagcgttca ctggtcgatg ccggtctgtt ggggaaaaag 840

aacggccgct cctattttgc cgccgaagaa accgccccgc cggtgacggc cgccaacaat 900

gcagacgtcg agacgctgcg cgtttacggc gagcaccctt tttttaccct gttgcagcag 960

cgagccgcgc ttcagtggcc acagctgtgc gtggaacaac ggccggcatt accggggctg 1020

ggagcggccg tccagataaa tgacgctttc accgtcagca tcaccgatgg ccgcacggcg 1080

agccaactgg ccgagcagac ggcagcggat gcctttgtga tcgatgtcgc cctgaactac 1140

gccgacacca cgtatctggt ggcagcgcac agccgccacg cttctgcggc caataaggcg 1200

ctgtttttac gcctgctgca cacggcaatc ccgcaggttg aatttatcaa ggactccccg 1260

gcgcttatcg tcgcccgcgt cctcagcagc ctgatcaatg agtcggtgat catggtggaa 1320

agcggcgtct gcagccggga agacatcgat gtcgccgccg tcgcgggcgt taactacgcc 1380

ggcggcattt tcgactggct cggcaagctg ggggagaaaa acgtcaggac gacgctgagc 1440

aatctggcgc agctgctgca cgcggcgcgc tatgcgccgc attacaccct cctgcacgcc 1500

gcgcaaccgg cgctgacgac cacgccttaa 1530

<210> 114

<211> 1530

<212> DNA

<213> Serratia marcescens 546

<400> 114

atggcagaaa gtaatgcggc aattcaatcg gctgcgatta tcggcgcggg aacgatgggc 60

agaggcatcg cttatctttt cgcgcaaaaa ggcattccca cgatgcttta taatcgcaac 120

ggcaataccc taaatcaagc tcgcgaatat atcgcgcaag acctgaacaa gaaagtcgaa 180

cagggcaaga tcgcgctgcg ggataaagac gcggtgctgg ccaatctgat gttcacttcc 240

gtgtttgagg ccatcgccga cagcgagctg gtgatagaaa ccatcgccga gcaagaacaa 300

accaaacttg aggtgctggc ggccatcgcc gcagtggtca agcccgacac gctgatcgcc 360

accaatacct cctcactgtc gcttaacaag ctgtcaactg cggtaacgca caacgaacgc 420

tttatcggtt tgcatttttt caaccccgcg ccgctgatga agctgattga aatcattccg 480

gcctacttta ccgcgcacgc caccacggaa cgctgccgcc aactggtggc ggcgttgggg 540

aaacgcgatg tcgtctgcca ggccacgccg gggttcatcg tcaatcgcat ggcccgcccc 600

tactacctgg aagggttccg cctgttggaa gaacatgtgg cgcgcgcggc gcagatcgac 660

cgcgccctca aggccggcgg gcgcttccgc atggggccgc tcgagctgac cgattttatc 720

ggccaagaca tcaactatca ggtcagccgg caaatctggc aggacatgca atacgacccg 780

cgctataccc ctggtcatct gcagcgttca ctggtcgatg ccggtctgtt ggggaaaaag 840

aacggccgct cctattttgc cgccgaagaa accgccccgc cggtgacggc cgccaacaat 900

gcagacgtcg agacgctgcg cgtttacggc gagcaccctt tttttaccct gttgcagcag 960

cgagccgcgc ttcagtggcc acagctgcgc gtggaacaac ggccggcatt accggggctg 1020

ggagcggccg tccggatcaa tgacgctttc accgtcagca tcaccgatgg ccgcacggcg 1080

agccaactgg ccgagcagac ggcagcggat gcctttgtgg tcgatgtcgc cctgaactac 1140

gccgacacca cgtatctggt ggcggcgcac agccgccacg cttctgcggc caataaggcg 1200

ctgtttttac gcctgctgca cacggcaatc ccgcaggttg aatttatcaa ggactctccg 1260

gcgcttatcg tcgcccgcgt cctcagcagc ctgatcaatg agtcggtgat catggtggaa 1320

agcggcgtct gcagccggga agacatcgat gtcgccgccg tcgcgggcgt taactacgcc 1380

ggcggcattt tcgactggct cggcaaactg ggggagaaaa acgtcaggac gacgctgagc 1440

aatctggcgc agctgctgca cgcggcgcgc tatgcgccgc attacaccct tctgcacgcc 1500

gcgcaaccgg cgctgacgac cacgccttaa 1530

<210> 115

<211> 1530

<212> DNA

<213> Serratia nematodiphila MB307

<400> 115

atggcagaaa gtaatgcggc aattcaatcg gctgcgatta tcggcgcggg aacgatgggc 60

agaggcatcg cttatctttt cgcgcaaaaa ggcattccca cgatgcttta taatcgcaac 120

ggcaataccc tcaatcaagc tcgcgaatat atcgcgcaag acctgaataa gaaagtcgaa 180

cagggcaaga tcgcgctgcg ggataaagac gcggtgctgg ccaatctgat gttcacttcc 240

gtgtttgagg ccatcgccga cagcgagctg gtgatagaaa ccatcgccga gcaagaacaa 300

accaaacttg aggtgctggc ggccatcgcc gcagtggtca agcccgacac gctgatcgcc 360

accaatacct cctcactgtc gctcaacaag ctggcaacgg cggtaacgca cagcgaacgc 420

tttatcggtt tgcatttttt caaccccgcg ccgctgatga agctgattga aatcattccg 480

gcctacttta ccgcgcacgc caccacggaa cgctgccgcc aactggtggc ggcgttgggg 540

aaacgcgatg tcgtctgcca ggccacgccg ggtttcatcg tcaatcgcat ggcccgcccc 600

tactacctgg aagggttccg cctgttggaa gaacatgtgg cgcgcgcggc gcagatcgac 660

cgcgccctca aggctggcgg gcgcttccgc atggggccgc tcgagctgac cgattttatc 720

ggccaagaca tcaactatca ggtcagccgg caaatctggc aggacatgca atacgacccg 780

cgctataccc ctggtcatct gcagcgttca ctggtcgatg ccggtctgtt ggggaaaaag 840

aacggccgct cctattttgc cgccgaagaa accgccccgc aggtgacggc cgccaacaat 900

gcagacgtcg agacgctgcg cgtttacggc gagcatcctt tttttaccct gttgcagcag 960

cgagccgcgc ttcagtggcc acagctgcgc gtggaacaac ggccggcatt accggggctg 1020

ggagcggccg tccagatcaa tgacgctttc accgtcagca tcaccgatgg ccgcacggcg 1080

agccaactgg ccgagcagac ggcagcggat gcctttgtgg tcgatctcgc cctgaactac 1140

gccgacacca cgtatctggt ggcggcgcac agccgccacg cttctgcggc caataaggcg 1200

ctgtttttac gcctgctgca cacggcaatc ccgcaggttg aatttatcaa ggactctccg 1260

gcgcttatcg tcgcccgcgt cctcagcagc ctgatcaatg agtcggtgat catggtggaa 1320

agcggcgtct gcagccggga agacatcgat gtcgccgccg tcgcgggcgt taactacgcc 1380

ggcggcattt tcgactggct cggcaaactg ggggagaaaa acgtcaggac gacgctgagc 1440

aatctggcgc agctgctgca cgcggcgcgc tatgcgccgc attacaccct tctgcacgcc 1500

gcgcaaccgg cgctgacgac cacgccttaa 1530

<210> 116

<211> 1530

<212> DNA

<213> Serratia marcescens VGH107

<400> 116

atggcagaaa gtaatgcggc aattcaatcg gctgcgatta tcggcgcggg aacgatgggc 60

agaggcatcg cttatctttt cgcgcaaaaa ggcattccca cgatgcttta taatcgcaac 120

ggcaataccc tcaatcaagc tcgcgaatat atcgcgcaag acctgaataa gaaagtcgaa 180

cagggcaaga tcgcgctgca ggataaggac gcggtgctgg ccaatctgat gttcacttcc 240

gtgtttgagg ccatcgccga cagcgagctg gtaatagaaa ccatcgccga gcaagaacaa 300

accaaacttg aggtgctggc ggccatcgcc gcggtggtca agcacgacac gctgatcgcc 360

accaatacct cctcactgtc gctcaacaag ctggcaactg cggtgacgca cagcgaacgc 420

tttatcggtt tgcatttttt caaccccgcg ccgctgatga agctgattga aatcattccg 480

gcctacttta ccgcgcacgc caccacggaa cgctgccgcc aactggtggc ggcgttgggg 540

aaacgcgatg tcgtctgcca ggccacgccg gggttcatcg tcaatcgcat ggcccgcccc 600

tactacctgg aagggttccg cctgttggaa gaacatgtgg cgcgcgcggc gcagatcgac 660

cgcgccctca aggccggcgg gcgcttccgc atggggccgc tcgagctgac cgattttatt 720

ggccaagaca ttaactatca ggtcagccgg caaatctggc aggacatgca atacgacccg 780

cgctataccc ctggtcatct gcagcgttca ctggtcgatg ccggtctgtt ggggaaaaag 840

aacggccgct cctattttgc cgccgaagaa accgccccgc cggtgacggc cgccaacaat 900

gcagacgtcg agacgctgcg cgtttacggc gagcatcctt tttttaccct gttgcagcag 960

cgagccgcgc ttcagtggcc acagctgcgc gtggaacaac ggccggcatt accggggctg 1020

ggagcggccg tccagatcaa tgacgctttc accgtaagca tcaccgatgg ccgcacggcg 1080

agccaactgg ccgagcagac ggcagcggat gcctttgtgg tcgatgtcgc cctgaactac 1140

gccgacacca cgtatctggt ggcggcgcac agccgccacg cttctgcggc caataaggcg 1200

ctgtttttgc gcctgctgca cacggcaatc ccgcaggttg aatttatcaa ggactctccg 1260

gcgcttatcg tcgcccgcgt cctcagcagc ctgatcaatg agtcggtgat catggtggaa 1320

agcggcgtct gcagccggga agacatcgat gtcgccgccg tcgcgggcgt taactacgcc 1380

ggcggcattt tcgactggct cggcaaactg ggggagaaaa acgtcaggac gatgctgagc 1440

aatctggcgc agctgctgca cgcggcgcgc tatgcgccgc attacaacct tctgcacgcc 1500

gcgcaaccgg cgctgacgac cacgccttaa 1530

<210> 117

<211> 1530

<212> DNA

<213> Serratia marcescens MCB

<400> 117

atggcagaaa gtaatgcggc aattcaatcg gctgcgatta tcggcgcggg aacgatgggc 60

agaggcatcg cttatctttt cgcgcaaaag ggcattcgta cggtgcttta taatcgcaac 120

ggcaataccc tcaatcaggc tcgcgaatat atcgcgcaag acctgaacaa gaaagtcgaa 180

cagggcaaga tcgcgctgca ggataaaagc tcagtgctgg ccaatctggt gttctcgacg 240

gcgcttgagg ctatcgccga cagcgagttg gtgatagaaa ccatcgccga gcatgaacaa 300

accaaacttg aggtgttggc ggccatcgcc gcggtggtca agcccgatac gctgatcgcc 360

accaatacct cctcactgtc gcttaacaag ctggcaaccg cggtgacgca cagcgagcgg 420

tttatcggtt tgcacttttt caaccctgcg ccgctgatga aactgattga aatcatcccg 480

gcctacttta ccgcacacgc caccacggaa cgttgccgtc aactggtggc cgcgttgggg 540

aaacgcgatg tcgtctgtca ggccacgccg gggtttatcg ttaaccgcat ggcccgcccc 600

tactacctgg aagggttccg cctgttggaa gaacacgtgg cgcgcgcgcc gcagatcgac 660

cgtgccctca aggccggcgg acacttccgc atggggccgc tcgagctgac cgatttcatc 720

ggccaggaca tcaactatca ggtcagtcgg caaatctggc aggacatgca atacgacccg 780

cgctataccc ccggccatct gcagcgttcg ctggtcgatg ccggtctgtt ggggaaaaag 840

aacggccgtt cctattttac cgccgaagaa accgccccgc cggtgacggc tgccatcggt 900

gcagacgtcg agacgctgcg catttacggc gaacatcctt tttttaccct gttgcaacag 960

cgggccgcac ttcagtggcc acagctgcgc gtggaacaac ggccggcctt accaggcgtg 1020

gggccggccg tccagatcaa tgacgcattc accgtcagca tcaccgacgg ccgtacggcg 1080

agccagttag ccgagcagac gacggctgac gcttttgtgg tcgatatcgc cctgaactac 1140

gccgacacgg cgtacctggt tgcggcgcac aatcgccacg cctctgcggc caataaggcg 1200

ctgtttttac gcctgttgca caccgcaatt ccgcaggttg aatttatcaa agactccccg 1260

gcgctgatcg tcgctcgcgt cctcagcagc ctgatcaatg agtcggtgat catggtggaa 1320

agcggcgttt gcagccggga agacattgat gtcgccgccg tcgcgggcgt taactacgcc 1380

ggcggcattt tcgactggct cagcaagctg ggggagaaaa acgtcaggac gacgctgagc 1440

aatctggcgc agttgctgca cgcggcgcgt tatgcgccgc attacaccct tctgcacgcc 1500

gcgcaaccgg cgctgacgac cacgccttga 1530

<210> 118

<211> 1530

<212> DNA

<213> Serratia marcescens AH0650

<400> 118

atggcagaaa gtaatgcggc aattcaatcg gctgcgatta tcggcgcggg aacgatgggc 60

agaggcatcg cttatctttt cgcgcaaaaa ggcattcgta cggtgcttta taatcgcaac 120

ggcaataccc tcaatcaggc tcgcgaatat atcgcgcaag acctgaacaa gaaagtcgaa 180

cagggcaaga tcgcgctgca ggataaaagc tcagtgctgg ccaatctggt gttctcgacg 240

gcgcttgagg ctatcgccga cagcgagttg gtgatagaaa ccatcgccga gcatgaacaa 300

accaaacttg aggtgttggc ggccatcgcc gcggtggtca agcccgatac gctgatcgcc 360

accaatacct cctcactgtc gcttaacaag ctggcaaccg cggtgacgca cagcgagcgg 420

tttatcggtt tgcacttttt caaccctgcg ccgctgatga aactgattga aatcatcccg 480

gcctacttta ccgcacacgc caccacggaa cgttgccgtc aactggtggc cgcgttgggg 540

aaacgcgatg tcgtctgtca ggccacgccg gggtttatcg ttaaccgcat ggcccgcccc 600

tactacctgg aagggttccg cctgttggaa gaacacgtgg cgcgcgcgcc gcagatcgac 660

cgtgccctca aggccggcgg acacttccgc atggggccgc tcgagctgac cgattttatc 720

ggccaggaca tcaactatca ggtcagccgg caaatctggc aggacatgca atacgacccg 780

cgctataccc ccggccatct gcagcgttcg ctggtcgatg ccggtctgtt ggggaaaaag 840

aacggccgct cctattttgc cgccgaagaa accgccccgc cagtgacggc cgccatcgat 900

gcagacgtcg agacgctgcg cgtttacggc gaacaccctt tttttaccct gttgcaacag 960

cgagccgcac gtcagtggcc acagctgcgc gtggaacaac ggccggcctt accgagcctg 1020

gggccggccg tccagatcaa tgacgcattc accgtcagca tcaccgatgg ccgcacggcg 1080

agccagttag ccgagcagac gacggctgac gcttttgtgg tcgacatcgc cctgaactac 1140

gccgacacgg cgtatctggt tgcggcgcac aatcgctatg cctctgcggc caataaggcg 1200

ctgtttttac gcctgctgca cactgcgatc ccgcaggttg aatttatcaa agactccccg 1260

gcgctgatcg tcgctcgtgt cctcagcagc ctgatcaatg agtcggtgat catggtggaa 1320

agcggcgttt gcagccggga agacattgat gtcgccgccg tcgcgggcgt taactacgcc 1380

ggcggcattt tcgactggct cggcaaactg ggggagaaaa acgtcaggac gacgctgagc 1440

aatctggcgc agctgctgca cgcagcgcgc tatgcgccgc attacaccct tctgcacgcc 1500

gcgcaaccgg cgctgacgac cacgccttga 1530

<210> 119

<211> 1530

<212> DNA

<213> Serratia marcescens UMH12

<400> 119

atggcagaaa gtaatgcgga aattcaatcg gctgcgatta tcggcgcggg aacgatgggc 60

agaggcatcg cttatctttt cgcgcaaaaa ggcattcgta cggtgcttta taatcgcaac 120

ggcaataccc tcaatcaggc tcgcgaatat atcgcgcaag acctgaacaa gaaagtcgaa 180

cagggcaaga tcgcgctgca ggataaaagc tcagtgctgg ccaatctggt gttctcgacg 240

gcgcttgagg ctatcgccga cagcgagttg gtgatagaaa ccatcgccga gcatgaacaa 300

accaaacttg aggtgttggc ggccatcgcc gcggtggtca agcccgatac gctgatcgcc 360

accaatacct cctcactgtc gcttaacaag ctggcaaccg cggtgacgca cagcgagcgg 420

tttatcggtt tgcacttttt caaccctgcg ccgctgatga aactgattga aatcatcccg 480

gcctacttta ccgcacacgc caccacggaa cgttgccgtc aactggtggc cgcgttgggg 540

aaacgcgatg tcgtctgtca ggccacgccg gggtttatcg ttaaccgcat ggcccgcccc 600

tactacctgg aagggttccg cctgttggaa gaacacgtgg cgcgcgcgcc gcagatcgac 660

cgtgccctca aggccggcgg acacttccgc atggggccgc tcgagctgac cgattttatc 720

ggccaggaca tcaactatca ggtcagccgg caaatctggc aggacatgca atacgacccg 780

cgctataccc ccggccatct gcagcgttcg ctggtcgatg ccggtctgtt ggggaaaaag 840

aacggccgct cctattttgc cgccgaagaa accgccccgc cagtgacggc cgccatcgat 900

gcagacgtcg agacgctgcg cgtttacggc gaacaccctt tttttaccct gttgcaacag 960

cgagccgcac gtcagtggcc acagctgcgc gtggaacaac ggccggcctt accgagcctg 1020

gggccggccg tccagatcaa tgacgcattc accgtcagca tcaccgatgg ccgcacggcg 1080

agccagttag ccgagcagac gacggctgac gcttttgtgg tcgacatcgc cctgaactac 1140

gccgacacgg cgtatctggt tgcggcgcac aatcgctatg cctctgcggc caataaggcg 1200

ctgtttttac gcctgctgca cactgcgatc ccgcaggttg aatttatcaa agactccccg 1260

gcgctgatcg tcgctcgtgt cctcagcagc ctgatcaatg agtcggtgat catggtggaa 1320

agcggcgttt gcagccggga agacattgat gtcgccgccg tcgcgggcgt taactacgcc 1380

ggcggcattt tcgactggct cggcaaactg ggggagaaaa acgtcaggac gacgctgagc 1440

aatctggcgc agctgctgca cgcagcgcgc tatgcgccgc attacaccct tctgcacgcc 1500

gcgcaaccgg cgctgacgac cacgccttga 1530

<210> 120

<211> 1530

<212> DNA

<213> Serratia sp. OMLW3

<400> 120

atggcagaaa gtaatgcggc aattcaatcg gctgcgatta tcggcgcggg aacgatgggc 60

agaggcatcg cttatctttt cgcgcaaaaa ggcattcgta cggtgcttta taatcgcaac 120

ggcaataccc tcaatcaggc tcgcgaatat atcgcgcaag acctgaacaa gaaagtcgaa 180

cagggcaaga tcgcgctgca ggataaaagc tcagtgctgg ccaatctggt gttctcgacg 240

gcgcttgtgg ctatcgccga cagcgagttg gtgatagaaa ccatcgccga gcatgaacaa 300

accaaacttg aggtgttggc ggccatcgcc gcggtggtca agcccgatac gctgatcgcc 360

accaatacct cctcactgtc gcttaacaag ctggcaaccg cggtgacgca cagcgagcgg 420

tttatcggtt tgcacttttt caaccctgcg ccgctgatga aactgattga aatcatcccg 480

gcctacttta ccgcacacgc caccacggaa cgttgccgtc aactggtggc cgcgttgggg 540

aaacgcgatg tcgtctgtca ggccacgccg gggtttatcg tcaaccgcat ggcccgcccc 600

tactacctgg aagggttccg cctgttggaa gagcacgtgg cgcgcgcgcc gcagatcgac 660

cgtgccctca aggccggcgg acacttccgc atggggccgc tcgagctgac cgattttatc 720

ggccaggaca tcaactatca ggtcagccgg caaatctggc aggacatgca atacgacccg 780

cgctataccc ccggccatct gcagcgttcg ctggtcgatg ccggtctgtt ggggaaaaag 840

aacggccgct cctattttgc cgccgaagaa accgccccgc cagtgacggc cgccatcgat 900

gcagacgtcg agacgctgcg cgtttacggc gaacaccctt tttttaccct gttgcaacag 960

cgagccgcac gtcagtggcc acagctgcgc gtggaacaac ggccggcctt accgagcctg 1020

gggccggccg tccagatcaa tgacgcattc accgtcagca tcaccgatgg ccgcacggcg 1080

agccagttag ccgagcagac gacggctgac gcttttgtgg tcgacatcgc cctgaactac 1140

gccgacacgg cgtatctggt tgcggcgcac aatcgctatg cctctgcggc caataaggcg 1200

ctgtttttac gcctgctgca cactgcgatc ccgcaggttg aatttatcaa agattccccg 1260

gcgctgatcg tcgctcgcgt cctcagcagc ctgatcaatg agtcggtgat catggtggaa 1320

agcggcgtct gcagccggga agacattgat gtcgccgccg tcgcgggcgt taactacgcc 1380

ggcggcattt tcgactggct cggcaaactg ggggagaaaa acgtcaggac gacgctgagc 1440

aatctggcgc agctgctgca cgcagcgcgc tatgcgccgc attacaccct tctgcacgcc 1500

gcgcaaccgg cgctgacgac cacgccttga 1530

<210> 121

<211> 1530

<212> DNA

<213> Serratia marcescens UMH11

<400> 121

atggcagaaa gtaatgcggc aattcaatcg gctgcgatta tcggcgcggg aacgatgggc 60

agaggcatcg cttatctttt cgcgcaaaaa ggcattcgta cggtgcttta taatcgcagc 120

ggcaataccc tcaatcaggc tcgcgaatat atcgcgcaag acctgaacaa gaaagtcgaa 180

cagggcaaga tcgcgctgca ggataaaagc tcagtgctgg ccaatctggt gttctcgacg 240

gcgcttgtgg ctatcgccga cagcgagttg gtgatagaaa ccatcgccga gcatgaacaa 300

accaaacttg aggtgttggc ggccatcgcc gcggtggtca agcccgatac gctgatcgcc 360

accaatacct cctcactgtc gcttaacaag ctggcaaccg cggtgacgca cagcgagcgg 420

tttatcggtt tgcacttttt caaccctgcg ccgctgatga aactgattga aatcatcccg 480

gcctacttta ccgcacacgc caccacggaa cgttgccgtc aactggtggc cgcgttgggg 540

aaacgcgatg tcgtctgtca ggccacgccg gggtttatcg tcaaccgcat ggcccgcccc 600

tactacctgg aagggttccg cctgttggaa gagcacgtgg cgcgcgcgcc gcagatcgac 660

cgtgccctca aggccggcgg acacttccgc atggggccgc tcgagctgac cgattttatc 720

ggccaggaca tcaactatca ggtcagccgg caaatctggc aggacatgca atacgacccg 780

cgctataccc ccggccatct gcagcgttcg ctggtcgatg ccggtctgtt ggggaaaaag 840

aacggccgct cctattttgc cgccgaagaa accgccccgc cagtgacggc cgccatcgat 900

gcagacgtcg agacgctgcg cgtttacggc gaacaccctt tttttaccct gttgcaacag 960

cgagccgcac gtcagtggcc acagctgcgc gtggaacaac ggccggcctt accgagcctg 1020

gggccggccg tccagatcaa tgacgcattc accgtcagca tcaccgatgg ccgcacggcg 1080

agccagttag ccgagcagac gacggctgac gcttttgtgg tcgacatcgc cctgaactac 1140

gccgacacgg cgtatctggt tgcggcgcac aatcgctatg cctctgcggc caataaggcg 1200

ctgtttttac gcctgctgca cactgcgatc ccgcaggttg aatttatcaa agattccccg 1260

gcgctgatcg tcgctcgcgt cctcagcagc ctgatcaatg agtcggtgat catggtggaa 1320

agcggcgtct gcagccggga agacattgat gtcgccgccg tcgcgggcgt taactacgcc 1380

ggcggcattt tcgactggct cggcaaactg ggggagaaaa acgtcaggac gacgctgagc 1440

aatctggcgc aactgctgca cgcagcgcgc tatgcgccgc attacaccct tctgcacgcc 1500

gcgcaaccgg cgctgacgac cacgccttga 1530

<210> 122

<211> 1530

<212> DNA

<213> Serratia marcescens UMH1

<400> 122

atggcagaaa gtaatgcggc aattcaatcg gctgcgatta tcggcgcggg aacgatgggc 60

agaggtatcg cttatctttt cgcgcaaaaa ggcattcgta cggtgcttta taatcgcaac 120

ggcaataccc tcaatcaggc tcgcgaatat atcgcgcaag acctgaacaa gaaagtcgaa 180

cagggcaaga tcgcgctgca ggataaaagc tcagtgctgg ccaatctggt gttctcgacg 240

gcgcttgagg ctatcgccga cagcgagttg gtgatagaaa ccatcgctga gcatgaacaa 300

accaaacttg aggtgttggc ggccatcgcc gcggtggtca agcccgatac gctgatcgcc 360

accaatacct cctcactgtc gcttaacaag ctggcaaccg cggtgacgca cagcgagcgg 420

tttatcggtt tgcacttttt caaccctgcg ccgctgatga aactgattga aatcatcccg 480

gcctacttta ccgcacatgc caccacggaa cgttgccgtc aactggtggc cgcgttgggg 540

aaacgcgatg tcgtctgcca ggccacgccg gggtttatcg ttaaccgcat ggcccgcccc 600

tactacctgg aagggttccg cctgttggaa gaacacgtgg cgcgcgcgcc gcagatcgac 660

cgtgccctca aggccggcgg acacttccgc atggggccgc tcgagctgac cgattttatc 720

ggccaggaca tcaactatca ggtcagccgg caaatctggc aggacatgca atacgacccg 780

cgctataccc ccggccatct gcagcgttcg ctggtcgatg ccggtctgtt ggggaaaaag 840

aacggccgct cctattttgc cgccgaagaa accgccccgc cagtgacggc cgccatcgat 900

gcagacgtcg agacgctgcg cgtttacggc gaacaccctt tttttaccct gttgcaacag 960

caagccgcac gtcagtggcc acagctgcgc gtggaacaac ggccggcctt accgagcctg 1020

gggccggccg tccagatcaa tggcgcattc accgtcagca tcaccgatgg ccgcacggcg 1080

agccagttag ccgagcagac gacggctgac gcttttgtga tcgacatcgc cctgaactac 1140

gccgacacgg cgtatctggt tgcggcgcac aatcgctatg cctctgcggc caataaggcg 1200

ctgtttttac gcctgctgca cactgcgatc ccgcaggttg aatttatcaa agactccccg 1260

gcgctgatcg tcgctcgtgt cctcagcagc ctgatcaatg agtcggtgat catggtggaa 1320

agcggcgttt gcagccggga agacattgat gtcgccgccg tcgcgggcgt taactacgcc 1380

ggcggcattt tcgactggct cggcaaactg ggggagaaaa acgtcaggac gacgctgagc 1440

aatctggcgc agctgctgca cgcagcgcgc tatgcgccgc attacaccct tctgcacgcc 1500

gcgcaaccgg cgctgacgac cacgccttga 1530

<210> 123

<211> 1530

<212> DNA

<213> Serratia marcescens 2880STDY5683020

<400> 123

atggcagaaa gtaatgcggc aattcaatcg gctgcgatta tcggcgcggg aacgatgggc 60

agaggcatcg cttatctttt cgcgcaaaaa ggcattcgca cggtgcttta taatcgcaac 120

ggcaataccc tcaatcaggc tcgcgaatat atcgcgcaag acctgaacaa gaaagtcgaa 180

cagggcaaga tcgcgctgca ggataaaggc gcagtgctgg ccaatctggt gttcacctcg 240

gtgtttgaga ctatcgccga cagcgatctg gtgatagaaa ccatcgccga gcaagagcaa 300

accaaacttg aggtgctggc ggccatcgcc gcggcggtca agcccgacac gctgatcgcc 360

accaatacct cctcactgtc gcttaacaag ctggcaaccg cggtgacgca cagcgagcgg 420

tttatcggtt tgcacttttt caatcccgcg ccgctgatga aactgattga aatcatcccg 480

gcctacttta ccgcacgcgc caccaccgaa cgttgccgtc agctggtagc cgcgttgggg 540

aaacgcgatg tcgtctgcca ggccacgccg gggtttatcg tcaaccgcat ggcccgcccc 600

tactacctgg aagggttccg cctgttggaa gaacacgtgg cgcgcgcgcc gcagatcgac 660

cgcgccctca aggccggcgg gcactttcgc atggggccgc tcgagctgac cgattttatc 720

ggccaggaca tcaactatca ggtcagccgg caaatctggc aggacatgca atacgatccg 780

cgctataccc ccggccatct gcagcgttcg ctggtcgatg ccggtctgtt ggggaaaaag 840

aacggccgct cctattttgc cgccgaagaa aacgccttac cggtaacggc cgccaccgat 900

gcagacgtcg agacgctgcg cgtttacggc gaacaccctt tttttaccct gttgcaacag 960

cgggccgcgc ttcagtggcc acggctgcgc gtggagcaac ggccggcctt accgggcctg 1020

gggccggccg tccagatcaa tgaggctttc accgtcagcg tcaccgatgg ccgcacggcg 1080

agccagttgg cggagcagac ggcggcggat gcctttgtgg tcgatgtcgc cctgaattac 1140

gccgacacgg cgtatctggt ggcagcgcac aaccgccacg cctctgcggc caataaggcg 1200

ctgtttttac gcctgctgca cacggcaatc ccgcaggttg aatttatcaa ggactccccg 1260

gcgctgatcg tcgcccgcgt cctcagcagc ctgatcaacg agtcggtgat catggtggaa 1320

agcggcgtct gcagccggga agacatcgat gtcgccgccg tcgccggcgt taactacgcc 1380

gacggcattt tcggctggct cgatagcctg ggggagaaaa acgtcaggac gacgctgagc 1440

aacctggcgc agctgctgca cgcggcacgc tatgcgccgc attacaccct tctgcacgcc 1500

gcgcaaccgg cgctgacgac cacgccttaa 1530

<210> 124

<211> 1530

<212> DNA

<213> Serratia marcescens 99

<400> 124

atggcagaaa gtaatgcggc aattcaatcg gctgcgatta tcggtgcggg aacgatgggc 60

agaggcatcg cttatctttt cgcgcaaaaa ggcattcgca cggtgcttta taatcgcaac 120

ggcaataccc tcaatcaggc tcgcgaatat atcgcgcaag acctgaacaa gaaagtcgaa 180

cagggcaaga tcgcgctgca ggataaaggc gcagtgctgg ccaatctggt gttcacctcg 240

gtgtttgaga ctatcgccga cagcgatctg gtgatagaaa ccatcgccga gcaagagcaa 300

accaaacttg aggtgctggc ggccatcgcc gcggcggtca agcccgacac gctgatcgcc 360

accaatacct cctcactgtc gcttaacaag ctggcaaccg cggtgacgca cagcgagcgg 420

tttatcggtt tgcacttttt caatcccgcg ccgctgatga aactgattga aatcatcccg 480

gcctacttta ccgcacgcgc caccaccgaa cgttgccgtc agctggtggc cgcgttgggg 540

aaacgcgatg tcgtctgcca ggccacgccg gggtttatcg tcaaccgcat ggcccgcccc 600

tactacctgg aagggttccg cctgttggaa gaacacgtgg cgcgcgcgcc gcagatcgac 660

cgcgccctca aggccggcgg gcactttcgc atggggccgc tcgagctgac cgattttatc 720

ggccaggaca tcaactatca ggtcagccgg caaatctggc aggacatgca atacgatccg 780

cgctataccc ccggccatct gcagcgttcg ctggtcgatg ccggtctgtt ggggaaaaag 840

aacggccgct cctattttgc cgccgaagaa aacgccttac cggtaacggc cgcctccaat 900

gcagacgtcg agacgctgcg cgtttacggc gaacaccctt tttttaccct gttgcaacag 960

cgggccgcgc ttcagtggcc acggctgcgc gtggagcaac ggccggcctt accgggtctg 1020

gggccggccg tccagatcaa tgaggctttc accgtcagcg tcaccgatgg ccgcacggcg 1080

agccaattgg cggagctgac ggcggcggat gcctttgtgg tcgatgtcgc cctgaactac 1140

gccgacacgg cgtatctggt ggcagcgcac aaccgccacg cctctgcggc caataaggcg 1200

ctgtttttac gcctgctgca cacggcaatc ccgcaggttg aatttatcaa ggactccccg 1260

gcgctgatcg tcgcccgcgt cctcagcagc ctgatcaacg agtcggtgat catggtggaa 1320

agcggcgtct gcagccggga agacatcgat gtcgccgccg tcgccggcgt taactacgcc 1380

gacggcattt tcggctggct cgatagcctg ggggagaaaa acgtcaggac gacgctgagc 1440

aacctggcgc agctgctgca cgcggcacgc tatgcgccgc attacaccct tctacacgcc 1500

gcgcaaccgg tgctgacgac cacgccttaa 1530

<210> 125

<211> 1530

<212> DNA

<213> Serratia marcescens 374

<400> 125

atggcagaaa gtaatgcggc aattcaatcg gctgcgatta tcggcgcggg aacgatgggc 60

agaggcatcg cttatctttt cgcgcaaaag ggcattcgta cggtgcttta taatcgcaac 120

ggcaataccc tcaatcaggc tcgcgaatat atcgcgcaag acctgaacaa gaaagtcgaa 180

cagggcaaga tcgcgctgca ggataaaagc tcagtgctgg ccaatctggt gttctcgacg 240

gcgcttgtgg ctatcgccga cagcgagttg gtgatagaaa ccatcgccga gcatgaacaa 300

accaaacttg aggtgttggc ggccatcgcc gcggtggtca agcccgatac gctgatcgcc 360

accaatacct cctcactgtc gcttaacaag ctggcaaccg cggtgacgca cagcgagcgg 420

tttatcggtt tgcacttttt caaccctgcg ccgctgatga aactgattga aatcatcccg 480

gcctacttta ccgcacacgc caccacggaa cgttgccgtc aactggtggc cgcgttgggg 540

aaacgcgatg tcgtctgtca ggccacgccg gggtttatcg tcaaccgcat ggcccgcccc 600

tactacctgg aagggttccg cctgttggaa gagcacgtgg cgcgcgcgcc gcagatcgac 660

cgtgccctca aggccggcgg acacttccgc atggggccgc tcgagctgac cgattttatc 720

ggccaggaca tcaactatca ggtcagccgg caaatctggc aggacatgca atacgacccg 780

cgctataccc ccggccatct gcagcgttcg ctggtcgatg ccggtctgtt ggggaaaaag 840

aacggccgct cctattttgc cgccgaagaa accgccccgc cagtgacggc cgccatcgat 900

gcagacgtcg agacgctgcg cgtttacggc gaacaccctt tttttaccct gttgcaacag 960

caagccgcac gtcagtggcc acagctgcgc gtggaacaac ggccggcctt accgagcctg 1020

gggccggccg tccagatcaa tggcgcattc accgtcagca tcaccgatgg ccgcacggcg 1080

agccagttag ccgagcagac gacggctgac gcttttgtga tcgacatcgc cctgaactac 1140

gccgacacgg cgtatctggt tgcggcgcac aatcgctatg cctctgcggc caataaggcg 1200

ctgtttttac gcctactgca cactgcgatc ccgcaggttg aatttatcaa agactccccg 1260

gcgctgatcg tcgctcgtgt cctcagcagc ctgatcaatg agtcggtgat catggtggaa 1320

agcggcgttt gcagccggga agacattgat gtcgccgccg tcgcgggcgt taactacgcc 1380

ggcggcattt tcgactggct cggcaaactg ggggagaaaa acgtcaggac gacgctgagc 1440

aatctggcgc agctgctgca cgcagcgcgc tatgcgccgc attacaccct tctgcacgcc 1500

gcgcaaccgg cgctgacgac cacgccttga 1530

<210> 126

<211> 1530

<212> DNA

<213> Serratia marcescens 2880STDY5683036

<400> 126

atggcagaaa gtaatgcggc aattcaatcg gctgcgatta tcggtgcggg aacgatgggc 60

agaggcatcg cttatctttt cgcgcaaaaa ggcattcgca cggtgcttta taatcgcaac 120

ggcaataccc tcaatcaggc tcgcgaatat atcgcgcaag acctgaacaa gaaagtcgaa 180

cagggcaaga tcgcgctgca ggataaaggc gcagtgctgg ccaatctggt gttcacctcg 240

gtgtttgaga ctatcgccga cagcgatctg gtgatagaaa ccatcgccga gcaagagcaa 300

accaaacttg aggtgctggc ggccatcgcc gcggcggtca agcccgacac gctgctcgcc 360

accaatacct cctcactgtc gcttaacaag ctggcaaccg cggtgacgca cagcgagcgg 420

tttatcggtt tgcacttttt caatcccgcg ccgctgatga aactgattga aatcatcccg 480

gcctacttta ccgcacgcgc caccaccgaa cgttgccgtc agctggtggc cgcgttgggg 540

aaacgcgatg tcgtctgcca ggccacgccg gggtttatcg tcaaccgcat ggcccgcccc 600

tactacctgg aagggttccg cctgttggaa gaacacgtgg cgcgcgcgcc gcagatcgac 660

cgcgccctca aggccggcgg gcactttcgc atggggccgc tcgagctgac cgattttatc 720

ggccaggaca tcaactatca ggtcagccgg caaatctggc aggacatgca atacgatccg 780

cgctataccc ccggccatct gcagcgttcg ctggtcgatg ccggtctgtt ggggaaaaag 840

aacggccgct cctattttgc cgccgaagaa aacgccttac cggtaacggc cgcctccaat 900

gcagacgtcg agacgctgcg cgtttacggc gaacaccctt tttttaccct gttgcaacag 960

cgggccgcgc ttcagtggcc acggctgcgc gtggagcaac ggccggcctt accgggtctg 1020

gggccggccg tccagatcaa tgaggctttc accgtcagcg tcaccgatgg ccgcacggcg 1080

agccaattgg cggagctgac ggcggcggat gcctttgtgg tcgatgtcgc cctgaactac 1140

gccgacacgg cgtatctggt ggcagcgcac aaccgccacg cctctgcggc caataaggcg 1200

ctgtttttac gcctgctgca cacggcaatc ccgcaggttg aatttatcaa ggactccccg 1260

gcgctgatcg tcgcccgcgt cctcagcagc ctgatcaacg agtcggtgat catggtggaa 1320

agcggcgtct gcagccggga agacatcgat gtcgccgccg tcgccggcgt taactacgcc 1380

gacggcattt tcggctggct cgatagcctg ggggagaaaa acgtcaggac gacgctgagc 1440

aacctggcgc agctgctgca cgcggcacgc tatgcgccgc attacaccct tctacacgcc 1500

gcgcaaccgg tgctgacgac cacgccttaa 1530

<210> 127

<211> 1530

<212> DNA

<213> Serratia marcescens 2880STDY5683034

<400> 127

atggcagaaa gtaatgcggc aattcaatcg gctgcgatta tcggcgcggg aacgatgggc 60

agaggcatcg cttatctttt cgcgcaaaaa ggcattcgca cggtgcttta taatcgcaac 120

ggcaataccc tcaatcaggc tcgcgaatat atcgcgcaag acctgaacaa gaaagtcgaa 180

cagggcaaga tcgcgctgca ggataaaggc gcagtgctgg ccaatctggt gttcacctcg 240

gtgtttgaga ctatcgccga cagcgagctg gtgatagaaa ccatcgccga gcaagagcaa 300

accaaacttg aggtgctggc ggccatcgcc gcggcggtca agcccgacac gctgatcgcc 360

accaatacct cctcactgtc gcttaacaag ctggcaaccg cggtgacgca cagcgagcgg 420

tttatcggtt tgcacttttt caatcccgcg ccgctgatga aactgattga aatcatcccg 480

gcctacttta ccgcacgcgc caccaccgaa cgctgccgtc agttggtggc cgcgttgggg 540

aaacgcgatg tcgtctgcca ggccacgccg gggtttatcg tcaaccgcat ggcccgcccc 600

tactacctgg aagggttccg cctgttggaa gaacacgtgg cgcgcgcgcc gcagatcgac 660

cgcgccctca aggccggcgg gcactttcgc atggggccgc tcgagctgac cgattttatc 720

ggccaggaca tcaactatca ggtcagccgg caaatctggc aggacatgca atacgatccg 780

cgctataccc ccggccatct gcagcgttcg ctggtcgatg ccggtctgtt ggggaaaaag 840

aacggccgct cctattttgc cgccgaagaa aacgccttac cggtaacggc cgccaccgat 900

gcagacgtcg agacgctgcg catttacggc gaacaccctt tttttaccct gttgcaacag 960

cgggccgcgc ttcagtggcc acggctgcgc gtggagcaac ggccggcctt accgggcctg 1020

gggccggccg tccagatcaa tgaggctttc accgtcagcg tcaccgatgg ccgcacggcg 1080

agccagttgg cggagctgac ggcggcggat gcctttgtgg tcgatgtcgc cctgaactac 1140

gccgacacgg cgtatctggt ggcagcgcac aaccgccacg cctctgcggc caataaggcg 1200

ctgtttttac gcctgctgca cacggcaatc ccgcaggttg aatttatcaa ggactccccg 1260

gcgctgatcg tcgcccgcgt cctcagcagc ctgatcaacg agtcggtgat catggtggaa 1320

agcggcgtct gcagccggga agacatcgat gtcgccgccg tcgccggcgt taactacgcc 1380

gacggcattt tcggctggct cgataacctg ggggagaaaa acgtcaggac gacgctgagc 1440

aacctggcgc agctgctgca cgcggcacgc tatgcgccgc attacaccct tctacacgcc 1500

gcgcaaccgg cgctgacgac cacgccttaa 1530

<210> 128

<211> 1530

<212> DNA

<213> Serratia marcescens 2880STDY5682892

<400> 128

atggcagaaa gtaatgcggc aattcaatcg gctgcgatta tcggtgcggg aacgatgggc 60

agaggcatcg cttatctttt cgcgcaaaaa ggcattcgca cggtgcttta taatcgcaac 120

ggcaataccc tcaatcaggc tcgcgaatat atcgcgcaag acctgaacaa gaaagtcgaa 180

cagggcaaga tcgcgctgca ggataaaggc gcagtgctgg ccaatctggt gttcacctcg 240

gtgtttgaga ctatcgccga cagcgatctg gtgatagaaa ccatcgccga gcaagagcaa 300

accaaacttg aggtgctggc ggccatcgcc gcggcggtca agcccgacac gctgatcgcc 360

accaatacct cctcactgtc gcttaacaag ctggcaaccg cggtgacgca cagcgagcgg 420

tttatcggtt tgcacttttt caatcccgcg ccgctgatga aactgattga aatcatcccg 480

gcctacttta ccgcacgcgc caccaccgaa cgttgccgtc agctggtagc cgcgttgggg 540

aaacgcgatg tcgtctgcca ggccacgccg gggtttatcg tcaaccgcat ggcccgcccc 600

tactacctgg aagggttccg cctgttggaa gaacacgtgg cgcgcgcgcc gcagatcgac 660

cgcgccctca aggccggcgg gcactttcgc atggggccgc tcgagctgac cgattttatc 720

ggccaggaca tcaactatca ggtcagccgg caaatctggc aggacatgca atacgatccg 780

cgctataccc ccggccatct gcagcgttcg ctggtcgatg ccggtctgtt ggggaaaaag 840

aacggccgct cctattttgc cgccgaagaa aacgccttac cggtaacggc cgccaccgat 900

gcagacgtcg agacgctgcg cgtttacggc gaacaccctt tttttaccct gttgcaacag 960

cgggccgcgc ttcagtggcc acggctgcgc gtggagcaac ggccggcctt accgggcctg 1020

gggccggccg tccagatcaa tgaggctttc accgtcagcg tcaccgatgg ccgcacggcg 1080

agccagttgg cggagctgac ggcggcggat gcctttgtgg tcgatgtcgc cctgaactac 1140

gccgacacgg cgtatctggt ggcagcgcac aaccgccacg cctctgcggc caataaggcg 1200

ctgtttttac gcctgctgca cacggcaatc ccgcaggttg aatttatcaa ggactccccg 1260

gcgctgatcg tcgcccgcgt cctcagcagc ctgatcaacg agtcggtgat catggtggaa 1320

agcggcgtct gcagccggga agacatcgat gtcgccgccg tcgccggcgt taactacgcc 1380

gacggcattt tcggctggct cgatagcctg ggggagaaaa acgtcaggac gacgctgagc 1440

aacctggcgc agctgctgca cgcggcacgc tatgcgccgc attacaccct tctgcacgcc 1500

gcgcaaccgg cgctgacgac cacgccttaa 1530

<210> 129

<211> 1530

<212> DNA

<213> Serratia marcescens SM39

<400> 129

atggcagaaa gtaatgcggc aattcaatcg gctgcgatta tcggcgcggg aacgatgggc 60

agaggcatcg cttatctttt cgcgcaaaaa ggcattcgca cggtgcttta taatcgcaac 120

ggcaataccc tcaatcaggc tcgcgaatat atcgtgcaag acctgaacaa gaaagtcgaa 180

cagggcaaga tcgcgctgca ggataaaggc gcagtgctgg ccaatctggt gttcacctcg 240

gtgtttgaga ctatcgccga cagcgagctg gtgatagaaa ccatcgccga gcaagagcaa 300

accaaacttg aggtgctggc ggccatcgcc gcggcggtca agcccgacac gctgatcgcc 360

accaatacct cctcactgtc gcttaacaag ctggcaaccg cggtgacgca cagcgagcgg 420

tttatcggtt tgcacttttt caatcccgcg ccgctgatga aactgattga aatcatcccg 480

gcctacttta ccgcacgcgc caccaccgaa cgttgccgtc agctggtggc cgcgttgggg 540

aaacgcgatg tcgtctgcca ggccacgccg gggtttatcg tcaaccgcat ggcccgcccc 600

tactacctgg aagggttccg cctgttggaa gaacacgtgg cgcgcgcgcc gcagatcgac 660

cgcgccctca aggccggcgg gcactttcgc atggggccgc tcgagctgac cgattttatc 720

ggccaggaca tcaactatca ggtcagccgg caaatctggc aggacatgca atacgatccg 780

cgctataccc ccggccatct gcagcgttcg ctggtcgatg ccggtctgtt ggggaaaaag 840

aacggccgct cctattttgc cgccgaagaa aacgccttac cggtaacggc cgccaccgat 900

gcagacgtcg agacgctgcg catttacggc gaacaccctt tttttaccct gttgcaacag 960

cgggccgcgc ttcagtggcc acggctgcgc gtggagcaac ggccggcctt accgggcctg 1020

gggccggccg tccagatcaa tgaggctttc accgtcagcg tcaccgatgg ccgcacggcg 1080

agccagttgg cggagctgac ggcggcggat gcctttgtgg tcgatgtcgc cctgaactac 1140

gccgacacgg cgtatctggt ggcagcgcac aaccgccacg cctctgcggc caataaggcg 1200

ctgtttttac gcctgctgca cacggcaatc ccgcaggttg aatttatcaa ggactccccg 1260

gcgctgatcg tcgcccgcgt cctcagcagc ctgatcaacg agtcggtgat catggtggaa 1320

agcggcgtct gcagccggga agacatcgat gtcgccgccg tcgccggcgt taactacgcc 1380

gacggcattt tcggctggct cgataacctg ggggagaaaa acgtcaggac gacgctgagc 1440

aacctggcgc agctgctgca cgcggcacgc tatgcgccgc attacaccct tctacacgcc 1500

gcgcaaccgg cgctgacgac cacgccttaa 1530

<210> 130

<211> 1530

<212> DNA

<213> Serratia marcescens 189

<400> 130

atggcagaaa gtaatgcggc aattcaatcg gctgcgatta tcggcgcggg aacgatgggc 60

agaggcatcg cttatctttt cgcgcaaaaa ggcattcgca cggtgcttta taatcgcaac 120

ggcaataccc tcaatcaggc tcgcgaatat atcgcgcaag acctgaacaa gaaagtcgaa 180

cagggcaaga tcgcgctgca ggataaaggc gcagtgctgg ccaatctggt gttcacctcg 240

gtgtttgaga ctatcgccga cagcgatctg gtgatagaaa ccatcgccga gcaagagcaa 300

accaaacttg aggtgctggc ggccatcgcc gcggcggtca agcccgacac gctgatcgcc 360

accaatacct cctcactgtc gcttaacaag ctggcaaccg cggtgacgca cagcgagcgg 420

tttatcggtt tgcacttttt caatcccgcg ccgctgatga aactgattga aatcatcccg 480

gcctacttta ccgcacgcgc caccaccgaa cgttgccgtc agctggtagc cgcgttgggg 540

aaacgcgatg tcgtctgcca ggccacgccg gggtttatcg tcaaccgcat ggcccgcccc 600

tactacctgg aagggttccg cctgttggaa gaacacgtgg cgcgcgcgcc gcagatcgac 660

cgcgccctca aggccggcgg gcactttcgc atggggccgc tcgagctgac cgattttatc 720

ggccaggaca tcaactatca ggtcagccgg caaatctggc aggacatgca atacgatccg 780

cgctataccc ccggccatct gcagcgttcg ctggtcgatg ccggtctgtt ggggaaaaag 840

aacggccgct cctattttgc cgccgaagaa aacgccttac cggtaacggc cgccaccgat 900

gcagacgtcg agacgctgcg catttacggc gaacaccctt tttttaccct gttgcaacag 960

cgggccgcgc ttcagtggcc acggctgcgc gtggagcaac ggccggcctt accgggcctg 1020

gggccggccg tccagatcaa tgaggctttc accgtcagcg tcaccgatgg ccgcacggcg 1080

agccagttgg cggagctgac ggcggcggat gcctttgtgg tcgatgtcgc cctgaactac 1140

gccgacacgg cgtatctggt ggcagcgcac aaccgccacg cctctgcggc caataaggcg 1200

ctgtttttac gcctgctgca cacggcaatc ccgcaggttg aatttatcaa ggactccccg 1260

gcgctgatcg tcgcccgcgt cctcagcagc ctgatcaacg agtcggtgat catggtggaa 1320

agcggcgtct gcagccggga agacatcgat gtcgccgccg tcgccggcgt taactacgcc 1380

gacggcattt tcggctggct cgataacctg ggggagaaaa acgtcaggac gacgctgagc 1440

aacctggcgc agctgctgca cgcggcacgc tatgcgccgc attacaccct tctacacgcc 1500

gcgcaaccgg cgctgacgac cacgccttaa 1530

<210> 131

<211> 1530

<212> DNA

<213> Serratia marcescens SMB2099

<400> 131

atggcagaaa gtaatgcggc aattcaatcg gctgcgatta tcggcgcggg aacgatgggc 60

agaggcatcg cttatctttt cgcgcaaaaa ggcattcgca cggtgcttta taatcgcaac 120

ggcaataccc tcaatcaggc tcgcgaatat atcgcgcaag acctgaacaa gaaagtcgaa 180

cagggcaaga tcgcgctgca ggataaaggc gcagtgctgg ccaatctggt gttcacctcg 240

gtgtttgaga ctatcgccga cagcgatctg gtgatagaaa ccatcgccga gcaagagcaa 300

accaaacttg aggtgctggc ggccatcgcc gcggcggtca agcccgacac gctgatcgcc 360

accaatacct cctcactgtc gcttaacaag ctggcaaccg cggtgacgca cagcgagcgg 420

tttatcggtt tgcacttttt caatcccgcg ccgctgatga aactgattga aatcatcccg 480

gcctacttta ccgcacgcgc caccaccgaa cgttgccgtc agctggtggc cgcgttgggg 540

aaacgcgatg tcgtctgcca ggccacgccg gggtttatcg tcaaccgcat ggcccgcccc 600

tactacctgg aagggttccg cctgttggaa gaacacgtgg cgcgcgcgcc gcagatcgac 660

cgcgccctca aggccggcgg gcactttcgc atggggccgc tcgagctgac cgattttatc 720

ggccaggaca tcaactatca ggtcagccgg caaatctggc aggacatgca atacgatccg 780

cgctataccc ccggccatct gcagcgttcg ctggtcgatg ccggtctgtt ggggaaaaag 840

aacggccgct cctattttgc cgccgaagaa aacgccttac cggtaacggc cgccaccgat 900

gcagacgtcg agacgctgcg cgtttacggc gaacaccctt tttttaccct gttgcaacag 960

cgggccgcgc ttcagtggcc acggctgcgc gtggagcaac ggccggcctt accgggcctg 1020

gggccggccg tccagatcaa tgaggctttc accgtcagcg tcaccgatgg ccgcacggcg 1080

agccagttgg cggagctgac ggcggcggat gcctttgtgg tcgatgtcgc cctgaactac 1140

gccgacacgg cgtatctggt ggcagcgcac aaccgccacg cctctgaggc caataaggcg 1200

ctgtttttac gcctgctgca cacggcaatc ccgcaggttg aatttatcaa ggactccccg 1260

gcgctgatcg tcgcccgcgt cctcagcagc ctgatcaacg agtcggtgat catggtggaa 1320

agcggcgtct gcagccggga agacatcgat gtcgccgccg tcgccggcgt taactacgcc 1380

gacggcattt tcggctggct cgatagcctg ggggagaaaa acgtcaggac gacgctgagc 1440

aacctggcgc agctgctgca cgcggcacgc tatgcgccgc attacaccct tctacacgcc 1500

gcgcaaccgg cgctgacgac cacgccttaa 1530

<210> 132

<211> 1530

<212> DNA

<213> Serratia marcescens 2880STDY5682862

<400> 132

atggcagaaa gtaatgcggc aattcaatcg gctgcgatta tcggcgcggg aacgatgggc 60

agaggcatcg cttatctttt cgcgcaaaaa ggcattcgca cggtgcttta taatcgcaac 120

ggcaataccc tcaatcaggc tcgcgaatat atcgtgcaag acctgaacaa gaaagtcgaa 180

cagggcaaga tcgcgctgca ggataaaggc gcagtgctgg ccaatctggt gttcacctcg 240

gtgtttgaga ccattgccga cagcgagctg gtgatagaaa ccatcgccga gcaagagcaa 300

accaaacttg aggtgctggc ggccatcgcc gcggcggtca agcccgacac gctgatcgcc 360

accaatacct cctcactgtc gcttaacaag ctggcaaccg cggtgacgca cagcgagcgg 420

tttatcggtt tgcacttttt caatcccgcg ccgctgatga aactgattga aatcatcccg 480

gcctacttta ccgcacgcgc caccaccgaa cgttgccgtc agctggtagc cgcgttgggg 540

aaacgcgatg tcgtctgcca ggccacgccg gggtttatcg tcaaccgcat ggcccgcccc 600

tactacctgg aagggttccg cctgttggaa gaacacgtgg cgcgcgcgcc gcagatcgac 660

cgcgccctca aggccggcgg gcactttcgc atggggccgc tcgagctgac cgattttatc 720

ggccaggaca tcaactatca ggtcagccgg caaatctggc aggacatgca atacgatccg 780

cgctataccc ccggccatct gcagcgttcg ctggtcgatg ccggtctgtt ggggaaaaag 840

aacggccgct cctattttgc cgccgaagaa aacgccttac cggtaacggc cgccaccgat 900

gcagacgtcg agacgctgcg cgtttacggc gaacaccctt tttttaccct gttgcaacag 960

cgggccgcgc ttcagtggcc acggctgcgc gtggagcaac ggccggcctt accgggcctg 1020

gggccggccg tccagatcaa tgaggctttc accgtcagcg tcaccgatgg ccgcacggcg 1080

agccagttgg cggagctgac ggcggcggat gcctttgtgg tcgatgtcgc cctgaactac 1140

gccgacacgg cgtatctggt ggcagcgcac aaccgccacg cctctgcggc caataaggcg 1200

ctgtttttac gcctgctgca cacggcaatc ccgcaggttg aatttatcaa ggactccccg 1260

gcgctgatcg tcgcccgcgt cctcagcagc ctgatcaacg agtcggtgat catggtggaa 1320

agcggcgtct gcagccggga agacatcgat gtcgccgccg tcgctggcgt taactacgcc 1380

gacggcattt tcggctggct cgatagcctg ggggagaaaa acgtcaggac gacgctgagc 1440

aacatggcgc agctgctgca cgcggcacgc tatgcgccgc attacaccct tctgcacgcc 1500

gcgcaaccgg cgctgatgac cacgccttaa 1530

<210> 133

<211> 1530

<212> DNA

<213> Serratia marcescens SE4145

<400> 133

atggcagaaa gtaatgcgga aattcaatcg gctgcgatta tcggcgcggg aacgatgggc 60

agaggcatcg cttatctttt cgcgcaaaaa ggcattcgca cggtgcttta taatcgcaac 120

ggcaataccc tcaatcaggc tcgcgaatat atcgtgcaag acctgaacaa gaaagtcgaa 180

cagggcaaga tcgcgctgca ggataaaggc gcagtgctgg ccaatctggt gttcacctcg 240

gtgtttgaga ctatcgccga cagcgagctg gtgatagaaa ccatcgccga gcaagagcaa 300

accaaacttg aggtgctggc ggccatcgcc gcggcggtca agcccgacac gctgatcgcc 360

accaatacct cctcactgtc gcttaacaag ctggcaaccg cggtgacgca cagcgagcgg 420

tttatcggtt tgcacttttt caatcccgcg ccgctgatga aactgattga aatcatcccg 480

gcctacttta ccgcacgcgc caccaccgaa cgttgccgtc agctggtagc cgcgttgggg 540

aaacgcgatg tcgtctgcca ggccacgccg gggtttatcg tcaaccgcat ggcccgcccc 600

tactacctgg aagggttccg cctgttggaa gaacacgtgg cgcgcgcgcc gcagatcgac 660

cgcgccctca aggccggcgg gcactttcgc atggggccgc tcgagctgac cgattttatc 720

ggccaggaca tcaactatca ggtcagccgg caaatctggc aggacatgca atacgatccg 780

cgctataccc ccggccatct gcagcgttcg ctggtcgatg ccggtctgtt ggggaaaaag 840

aacggccgct cctattttgc cgccgaagaa aacgccttac cggtaacggc cgccaccgat 900

gcagacgtcg agacgctgcg catttacggc gaacaccctt tttttaccct gttgcaacag 960

cgggccgcgc ttcagtggcc acggctgcgc gtggagcaac ggccggcctt accgggcctg 1020

gggccggccg tccagatcaa tgaggctttc accgtcagcg tcaccgatgg ccgcacggcg 1080

agccagttgg cggagctgac ggcggcggat gcctttgtgg tcgatgtcgc cctgaactac 1140

gccgacacgg cgtatctggt ggcagcgcac aaccgccacg cctctgcggc caataaggcg 1200

ctgtttttac gcctgctgca cacggcaatc ccgcaggttg aatttatcaa ggactccccg 1260

gcgctgatcg tcgcccgcgt cctcagcagc ctgatcaacg agtcggtgat catggtggaa 1320

agcggcgtct gcagccggga agacatcgat gtcgccgccg tcgccggcgt taactacgcc 1380

gacggcattt tcggctggct cgataacctg ggggagaaaa acgtcaggac gacgctgagc 1440

aacctggcgc agctgctgca cgcggcacgc tatgcgccgc attacaccct tctacacgcc 1500

gcgcaaccgg cgctgacgac cacgccttaa 1530

<210> 134

<211> 1530

<212> DNA

<213> Serratia marcescens 2880STDY5682876

<400> 134

atggcagaaa gtaatgcggc aattcaatcg gctgcgatta tcggtgcggg aacgatgggc 60

agaggcatcg cttatctttt cgcgcaaaaa ggcattcgca cggtgcttta taatcgcaac 120

ggcaataccc tcaatcaggc tcgcgaatat atcgcgcaag acctgaacaa gaaagtcgaa 180

cagggcaaga tcgcgctgca gggtaaaggc gcagtgctgg ccaatctggt gttcacctcg 240

gtgtttgaga ctatcgccga cagcgatctg gtgatagaaa ccatcgccga gcaagagcaa 300

accaaacttg aggtgctggc ggccatcgcc gcggcggtca agcccgacac gctgatcgcc 360

accaatacct cctcactgtc gcttaacaag ctggcaaccg cggtgacgca cagcgagcgg 420

tttatcggtt tgcacttttt caatcccgcg ccgctgatga aactgattga aatcatcccg 480

gcctacttta ccgcacgcgc caccaccgaa cgttgccgtc agctggtagc cgcgttgggg 540

aaacgcgatg tcgtctgcca ggccacgccg gggtttatcg tcaaccgcat ggcccgcccc 600

tactacctgg aagggttccg cctgttggaa gaacacgtgg cgcgcgcgcc gcagatcgac 660

cgcgccctca aggccggcgg gcactttcgc atggggccgc tcgagctgac cgattttatc 720

ggccaggaca tcaactatca ggtcagccgg caaatctggc aggacatgca atacgatccg 780

cgctataccc ccggccatct gcagcgttcg ctggtcgatg ccggtctgtt ggggaaaaag 840

aacggccgct cctattttgc cgccgaagaa aacgccttac cggtaacggc cgccaccgat 900

gcagacgtcg agacgctgcg cgtttacggc gaacaccctt tttttaccct gttgcaacag 960

cgggccgcgc ttcagtggcc acggctgcgc gtggagcaac ggccggcctt accgggcctg 1020

gggccggccg tccagatcaa tgaggctttc accgtcagcg tcaccgatgg ccgcacggcg 1080

agccagttgg cggagctgac ggcggcggat gcctttgtgg tcgatgtcgc cctgaactac 1140

gccgacacgg cgtatctggt ggcagcgcac aaccgccacg cctctgcggc caataaggcg 1200

ctgtttttac gcctgctgca cacggcaatc ccgcaggttg aatttatcaa ggactccccg 1260

gcgctgatcg tcgcccgcgt cctcagcagc ctgatcaacg agtcggtgat catggtggaa 1320

agcggcgtct gcagccggga agacatcgat gtcgccgccg tcgccggcgt taactacgcc 1380

gacggcattt tcggctggct cgatagcctg ggggagaaaa acgtcaggac gacgctgagc 1440

aacctggcgc agctgctgca cgcggcacgc tatgcgccgc attacaccct tctgcacgcc 1500

gcgcaaccgg cgctgacgac cacgccttaa 1530

<210> 135

<211> 1530

<212> DNA

<213> Serratia marcescens 709

<400> 135

atggcagaaa gtaatgcggc aattcaatcg gctgcgatta tcggcgcggg aacgatgggc 60

agaggcatcg cttatctttt cgcgcaaaaa ggcattcgca cggtgcttta taatcgcaac 120

ggcaataccc tcaatcaggc tcgcgaatat atcgcgcaag acctgaacaa gaaagtcgaa 180

cagggcaaga tcgcgctgca ggataaaggc gcagtgctgg ccaatctggt gttcacctcg 240

gtgtttgaga ctatcgccga cagcgatctg gtgatagaaa ccatcgccga gcaagagcaa 300

accaaacttg aggtgctggc ggccatcgcc gcggcggtca agcccgacac gctgatcgcc 360

accaatacct cctcactgtc gcttaacaag ctggcaaccg cggtgacgca cagcgagcgg 420

tttatcggtt tgcacttttt caatcccgcg ccgctgatga aactgattga aatcatcccg 480

gcctacttta ccgcacgcgc caccaccgaa cgttgccgtc agctggtagc cgcgttgggg 540

aaacgcgatg tcgtctgcca ggccacgccg gggtttatcg tcaaccgcat ggcccgcccc 600

tactacctgg aagggttccg cctgttggaa gaacacgtgg cgcgcgcgcc gcagatcgac 660

cgcgccctca aggccggcgg gcactttcgc atggggccgc tcgagctgac cgattttatc 720

ggccaggaca tcaactatca ggtcagtcgg caaatctggc aggacatgca atacgatccg 780

cgctataccc ccggccatct gcagcgttcg ctggtcgatg ccggtctgtt ggggaaaaag 840

aacggccgct cctattttgc cgccgaagaa aacgccttac cggtaacggc cgccaccgat 900

gcagacgtcg agacgctgcg cgtttacgac gaacaccctt tttttaccct gttgcaacag 960

cgggccgcgc ttcagtggcc acggctgcgc gtggagcaac ggccggcctt accgggcctg 1020

gggccggccg tccagatcaa tgaggctttc accgtcagcg tcaccgatgg ccgcacggcg 1080

agccagttgg cggagctgac ggcggcggat gcctttgtgg tcgatgtcgc cctgaactac 1140

gccgacacgg cgtatctggt ggcagcgcac aaccgccacg cctctgcggc caataaggcg 1200

ctgtttttac gcctgctgca cacggcaatc ccgcaggttg aatttatcaa ggactccccg 1260

gcgctgatcg tcgcccgcgt cctcagcagc ctgatcaacg agtcggtgat catggtggaa 1320

agcggcgtct gcagccggga agacatcgat gtcgccgccg tcgccggcgt taactacgcc 1380

gacggcattt tcggctggct cgatagcctg ggggagaaaa acgtcaggac gacgctgagc 1440

aacctggcgc agctgctgca cgcggcacgc tatgcgccgc attacaccct tctgcacgcc 1500

gcgcaaccgg cgctgacgac cacgccttaa 1530

<210> 136

<211> 1530

<212> DNA

<213> Serratia marcescens MGH136

<400> 136

atggcagaaa gtaatgcggc aattcaatcg gctgcgatta tcggcgcggg aacgatgggc 60

agaggcatcg cttatctttt cgcgcaaaaa ggcattcgca cggtgcttta taatcgcaac 120

ggcaataccc tcaatcaggc tcgcgaatat atcgcgcaag acctgaacaa gaaagtcgaa 180

caggacaaga tcgcgctgca ggataaaggc gcagtgctgg ccaatctggt gttcacctcg 240

gtgtttgaga ctatcgccga cagcgatctg gtgatagaaa ccatcgccga gcaagagcaa 300

accaaacttg aggtgctggc ggccatcgcc gcggcggtta agcccgacac gctgatcgcc 360

accaatacct cctcactgtc gcttaacaag ctggcaaccg cggtgacgca cagcgagcgg 420

tttatcggtt tgcacttttt caatcccgcg ccgctgatga aactgattga aatcatcccg 480

gcctacttta ccgcacgcgt caccaccgaa cgttgccgtc agctggtggc cgcgttgggg 540

aaacgcgatg tcgtctgcca ggccacgccg gggtttatcg tcaaccgcat ggcccgcccc 600

tactacctgg aagggttccg tctgttagaa gaacacgtgg cgcgcgcgcc gcagatcgac 660

cgcgccctca aggccggcgg gcactttcgc atggggccgc tcgagctgac cgattttatc 720

ggccaggaca tcaactatca ggtcagccgg caaatctggc aggacatgca atacgatccg 780

cgctataccc ccggccatct gcagcgttcg ctggtcgatg ccggtctgtt ggggaaaaag 840

aacggccgct cctattttgc cgccgaagaa aacgccttac cggtaacggc cgccaccgat 900

gcagacgtcg agacgctgcg cgtttacggc gaacaccctt tttttaccct gttgcaacag 960

cgggccgcgc ttcagtggcc acggctgcgc gtggagcaac ggccggcctt accgggcctg 1020

gggccggccg tccagatcaa tgaggctttc accgtcagcg tcaccgatgg ccgcacggcg 1080

agccagttgg cggagctgac ggcggcggat gcctttgtgg tcgatgtcgc cctgaactac 1140

gccgacacgg cgtatctggt ggcagcgcac aaccgccacg cctctgcggc caataaggcg 1200

ctgtttttac gcctgctgca cacggcaatc ccgcaggttg aatttatcaa ggactccccg 1260

gcgctgatcg tcgcccgcgt cctcagcagc ctgatcaacg agtcggtgat catggtggaa 1320

agcggcgtct gcagccggga agacatcgat gtcgccgctg tcgccggcgt taactacgcc 1380

gacggcattt tcggctggct cgatagcctg ggggagaaaa acgtcagaac gacgctgagc 1440

aacctggcgc agctgctgca cgcggcacgc tatgcgccgc attacaccct tctgcacgcc 1500

gcgcaaccgg cgctgacgac cacgccttaa 1530

<210> 137

<211> 1530

<212> DNA

<213> Serratia marcescens 2880STDY5682884

<400> 137

atggcagaaa gtaatgcggc aattcaatcg gctgcgatta tcggcgcggg aacgatgggc 60

agaggcatcg cttatctttt cgcgcaaaaa ggcattcgca cggtgcttta taatcgcaac 120

ggcaataccc tcaatcaggc tcgcgaatat atcgcgcaag acctgaacaa gaaagtcgaa 180

cagggcaaga tcgcgctgca ggataaaggc gcagtgctgg ccaatctggt gttcacctcg 240

gtgtttgaga ctatcgccga cagcgagctg gtgatagaaa ccatcgccga gcaagagcaa 300

accaaacttg aggtgctggc ggccatcgcc gcggcggtca agcccgacac gctgatcgcc 360

accaatacct cctcactgtc gcttaacaag ctggcaaccg cggtgacgca cagcgagcgg 420

tttatcggtt tgcacttttt caatcccgcg ccgctgatga aactgattga aatcatcccg 480

gcctacttta ccgcacgcgc caccaccgaa cgctgccgtc agttggtggc cgcgttgggg 540

aaacgcgatg tcgtctgcca ggccacgccg gggtttatcg tcaaccgcat ggcccgcccc 600

tactacctgg aagggttccg cctgttggaa gaacacgtgg cgcgcgcgcc gcagatcgac 660

cgcgccctca aggccggcgg gcactttcgc atggggccgc tcgagctgac cgattttatc 720

ggccaggaca tcaactatca ggtcagccgg caaatctggc aggacatgca atacgatccg 780

cgctataccc ccggccatct gcagcgttcg ctggtcgatg ccggtctgtt ggggaaaaag 840

aacggccgct cctattttgc cgccgaagaa aacgccttac cggtaacggc cgccaccgat 900

gcagacgtcg agacgctgcg catttacggc gaacaccctt tttttaccct gttgcaacag 960

cgggccacgc ttcagtggcc acggctgcgc gtggagcaac ggccggcctt accgggcctg 1020

gggccggccg tccagatcaa tgaggctttc accgtcagcg tcaccgatgg ccgcacggcg 1080

agccagttgg cggagctgat ggcggcggat gcctttgtgg tcgatgtcgc cctgaactac 1140

gccgacacgg cgtatctggt ggcagcgcac aaccgccacg cctctgcggc caataaggcg 1200

ctgtttttac gcctgctgca cacggcaatc ccgcaggttg aatttatcaa ggactccccg 1260

gcgctgatcg tcgcccgcgt cctcagcagc ctgatcaacg agtcggtgat catggtggaa 1320

agcggcgtct gcagtcggga agacatcgat gtcgccgccg tcgccggcgt taactacgcc 1380

gacggcattt tcggctggct cgatagcctg ggggagaaaa acgtcaggat gacgctgagc 1440

aacctggcgc agctgctgca cgcggcacgc tatgcgccgc attacaccct tctacacgcc 1500

gcgcaaccgg cgctgacgac cacgccttaa 1530

<210> 138

<211> 1530

<212> DNA

<213> Serratia marcescens D-3

<400> 138

atggcagaaa gtaatgcggc aattcaatcg gctgcgatta tcggcgcggg aacgatgggc 60

agaggcatcg cttatctttt cgcgcaaaaa ggcattcgca cggtgcttta taatcgcaac 120

ggcaataccc tcaatcaggc tcgcgaatat atcgcgcaag acctgaacaa gaaagtcgaa 180

cagggcaaga tcgcgctgca ggataaaggc gcagtgctgg ccaatctggt gttcacctcg 240

gtgtttgaga ctatcgccga cagcgatctg gtgatagaaa ccatcgccga gcaagagcaa 300

accaaacttg aggtgctggc ggccatcgcc gcggcggtca agcccgacac gctgatcgcc 360

accaatacct cctcactgtc gcttaacaag ctggcaaccg cggtgacgca cagcgagcgg 420

tttatcggtt tgcacttttt caatcccgcg ccgctgatga aactgattga aatcatcccg 480

gcctacttta ccgcacgcgc caccaccgaa cgttgccgtc agctggtggc cgcgttgggg 540

aaacgcgatg tcgtctgcca ggccacgccg gggtttatcg tcaaccgcat ggcccgcccc 600

tactacctgg aagggttccg cctgttggaa gaacacgtgg cgcgcgcgcc gcagatcgac 660

cgcgccctca aggccggcgg gcactttcgc atggggccgc tcgagctgac cgattttatc 720

ggccaggaca tcaactatca ggtcagccgg caaatctggc aggacatgca atacgatccg 780

cgctataccc ccggccatct gcagcgttcg ctggtcgatg ccggtctgtt ggggaaaaag 840

aacggccgct cctattttgc cgccgaagaa aacgccttac cggtaacggc cgccatcgat 900

gcagacgtcg agacgctgcg cgtttacggc gaacaccctt tttttaccct gttgcaacag 960

cgggccgcgc ttcagtggcc acggctgcgc gtggagcaac ggccggcctt accgggcctg 1020

gggccggccg tccagatcaa tgaggctttc accgtcagcg tcaccgatgg ccgcacggcg 1080

agccagttgg cggagctgac ggcggcggat gcctttgtgg tcgatgtcgc cctgaactac 1140

gccgacacgg cgtatctggt ggcagcgcac aaccgccacg cctctgcggc caataaggcg 1200

ctgtttttac gcctgctgca cacggcaatc ccgcaggttg aatttatcaa ggactccccg 1260

gcgctgatcg tcgcccgcgt cctcagcagc ctgatcaacg agtcggtgat catggtggaa 1320

agcggcgtct gcagccggga agacatcgat gtcgccgccg tcgccggcgt taactacgcc 1380

gacggcattt tcggcttgct cgatagcctg ggggagaaaa acgtcaggac gacgctgagc 1440

aacctggcgc agctgctgca cgcggcacgc tatgcgccgc attacaccct tctacacgcc 1500

gcgcaaccgg cgctgacgac cacgccttaa 1530

<210> 139

<211> 1530

<212> DNA

<213> Serratia marcescens 2880STDY5682957

<400> 139

atggcagaaa gtaatgcggc aattcaatcg gctgcgatta tcggcgcggg aacgatgggc 60

agaggcatcg cttatctttt cgcacaaaaa ggcattcgca cggtgcttta taatcgcaac 120

ggcaataccc tcaatcaggc tcgcgaatat atcgagctag acctgaacaa gaaagtcgaa 180

cagggcaaga tcgcgctgca ggataaaggc gcggtgctgg ccaatctggt gttcacctcg 240

gcgtttgaga ccattgccga cagcgagctg gtgatagaaa ccatcgccga gcaagagcaa 300

accaaacttg aggtgctggc ggccatcgcc gcggcggtca agcccgacac gctgatcgcc 360

accaatacct cctcactgtc gcttaacaag ctggcaaccg cggtgacgca cagcgagcgg 420

tttatcggtt tgcacttttt caatcccgcg ccgctgatga aactgattga aatcatcccg 480

gcctacttta ccgcacgcgc caccaccgaa cgttgccgtc agctggtagc cgcgttgggg 540

aaacgcgatg tcgtctgcca ggccacgccg gggtttatcg tcaaccgcat ggcccgcccc 600

tactacctgg aagggttccg cctgttggaa gaacacgtgg cgcgcgcgcc gcagatcgac 660

cgcgccctca aggccggcgg gcactttcgc atggggccgc tcgagctgac cgattttatc 720

ggccaggaca tcaactatca ggtcagccgg caaatctggc aggacatgca atacgatccg 780

cgctataccc ccggccatct gcagcgttcg ctggtcgatg ccggtctgtt ggggaaaaag 840

aacggccgct cctattttgc cgccgaagaa aacgccttac cggtaacggc cgccaccgat 900

gcagacgtcg agacgctgcg cgtttacggc gaacaccctt tttttaccct gttgcaacag 960

cgggccgcgc ttcagtggcc acggctgcgc gtggagcaac ggccggcctt accgggcctg 1020

gggccggccg tccagatcaa tgaggctttc accgtcagcg tcaccgatgg ccgcacggcg 1080

agccagttgg cggagctgac ggcggcggat gcctttgtgg tcgatgtcgc cctgaactac 1140

gccgacacgg cgtatctggt ggcagcgcac aaccgccacg cctctgcggc caataaggcg 1200

ctgtttttac gcctgctgca cacggcaatc ccgcaggttg aatttatcaa ggactccccg 1260

gcgctgatcg tcgcccgcgt cctcagcagc ctgatcaacg agtcggtgat catggtggaa 1320

agcggcgtct gcagccggga agacatcgat gtcgccgccg tcgccggcgt taactacgcc 1380

gacggtattt tcggctggct cgatagcctg ggggagaaaa acgtcaggac gacgctgagc 1440

aacctggcgc agctgctgca cgcggcacgc tatgcgccgc attacaccct tctacacgcc 1500

gcgcaaccgg cgctgacgac cacgccttaa 1530

<210> 140

<211> 1530

<212> DNA

<213> Serratia marcescens YDC563

<400> 140

atggcagaaa gtaatgcagc aattcagtcg gctgcgatta tcggcgcggg aacgatgggc 60

agaggcatcg cttatctttt cgcacaaaaa ggcattcgca cggtgcttta taatcgcaac 120

ggcaataccc tcaatcaggc tcgcgaatat atcgagctag acctgaacaa gaaagtcgaa 180

cagggcaaga tcgcgctgca ggataaaggc gcggtgctgg ccaatctggt gttcacctcg 240

gcgtttgaga ccattgccga cagcgagctg gtgatagaaa ccatcgccga gcaagagcaa 300

accaaacttg aggtgctggc ggccatcgcc gcggcggtca agcccgacac gctgatcgcc 360

accaatacct cctcactgtc gcttaacaag ctggcaaccg cggtgacgca cagcgagcgg 420

tttatcggtt tgcacttttt caatcccgcg ccgctgatga aactgattga aatcatcccg 480

gcctacttta ccgcacgcgc caccaccgaa cgttgccgtc agctggtagc cgcgttgggg 540

aaacgcgatg tcgtctgcca ggccacgccg gggtttatcg tcaaccgcat ggcccgcccc 600

tactacctgg aagggttccg cctgttggaa gaacacgtgg cgcgcgcgcc gcagatcgac 660

cgcgccctca aggccggcgg gcactttcgc atggggccgc tcgagctgac cgattttatc 720

ggccaggaca tcaactatca ggtcagccgg caaatctggc aggacatgca atacgatccg 780

cgctataccc ccggccatct gcagcgttcg ctggtcgatg ccggtctgtt ggggaaaaag 840

aacggccgct cctattttgc cgccgaagaa aacgccttac cggtaacggc cgccaccgat 900

gcagacgtcg agacgctgcg cgtttacggc gaacaccctt tttttaccct gttgcaacag 960

cgggccgcgc ttcagtggcc acggctgcgc gtggagcaac ggccggcctt accgggcctg 1020

gggccggccg tccagatcaa tgaggctttc accgtcagcg tcaccgatgg ccgcacggcg 1080

agccagttgg cggagcagac ggcggcggat gcctttgtgg tcgatgtcgc cctgaattac 1140

gccgacacgg cgtatctggt ggcagcgcac aaccgccacg cctctgcggc caataaggcg 1200

ctgtttttac gcctgctgca cacggcaatc ccgcaggttg aatttatcaa ggactccccg 1260

gcgctgatcg tcgcccgcgt cctcagcagc ctgatcaacg agtcggtgat catggtggaa 1320

agcggcgtct gcagccggga agacatcgat gtcgccgccg tcgctggcgt taactacgcc 1380

gacggcattt tcggctggct cgatagcctg ggggagaaaa acgtcaggac gacgctgagc 1440

aacatggcgc agctgctgca cgcggcacgc tatgcgccgc attacaccct tctgcacgcc 1500

gcgcaaccgg cgctgatgac cacgccttaa 1530

<210> 141

<211> 1530

<212> DNA

<213> Serratia marcescens 2880STDY5683035

<400> 141

atggcagaaa gtaatgcggc aattcaatcg gctgcgatta tcggcgcggg aacgatgggc 60

agaggcatcg cttatctttt cgcgcaaaaa ggcattcgca cggtgcttta taatcgcaac 120

ggcaataccc tcaatcaggc tcgcgaatat atcgcgcaag acctgaacaa gaaagtcgaa 180

cagggcaaga tcgcgctgca ggataaaggc gcagtgctgg ccaatctggt gttcacctcg 240

gtgtttgaga ctatcgccga cagcgatctg gtgatagaaa ccatcgccga gcaagagcaa 300

accaaacttg aggtgctggc ggccatcgcc gcggcggtca agcccgacac gctgatcgcc 360

accaatacct cctcactgtc gcttaacaag ctggcaaccg cggtgacgca cagcgagcgg 420

tttatcggtt tgcacttttt caatcccgcg ccgctgatga aactgattga aatcatcccg 480

gcctacttta ccgcacgcgc caccaccgaa cgttgccgtc agctggtggc cgcgttgggg 540

aaacgcgatg tcgtctgcca ggccacgccg gggtttatcg tcaaccgcat ggcccgcccc 600

tactacctgg aagggttccg cctgttggaa gaacacgtgg cgcgcgcgcc gcagatcgac 660

cgcgccctca aggccggcgg gcactttcgc atggggccgc tcgagctgac cgattttatc 720

ggccaggaca tcaactatca ggtcagccgg caaatctggc aggacatgca atacgatccg 780

cgctataccc ccggccatct gcagcgttcg ctggtcgatg ccggtctgtt ggggaaaaag 840

aacggccgct cctattttgc cgccgaagaa aacgccttac cggtaacggc cgccatcgat 900

gcagacgtcg agacgctgcg cgtttacggc gaacaccctt tttttaccct gttgcaacag 960

cgggccgcgc ttcagtggcc acggctgcgc gtggagcaac ggccggcctt accgggcctg 1020

gggccggccg tccagatcaa tgaggctttc accgtcagcg tcaccgatgg ccgcacggcg 1080

agccagttgg cggagctgac ggcggcggat gtctttgtgg tcgatgtcgc cctgaactac 1140

gccgacacgg cgtatctggt ggcagcgcac aaccgccacg cctctgcggc caataaggcg 1200

ctgtttttac gcctgctgca cacggcaatc ccgcaggttg aatttatcaa ggactccccg 1260

gcgctgatcg tcgcccgcgt cctcagcagc ctgatcaacg agtcggtgat catggtggaa 1320

agcggcgtct gcagccggga agacatcgat gtcgccgccg tcgccggcgt taactacgcc 1380

gacggcattt tcggcttgct cgatagcctg ggggagaaaa acgtcaggac gacgctgagc 1440

aacctggcgc agctgctgca cgcggcacgc tatgcgccgc attacaccct tctacacgcc 1500

gcgcaaccgg cgctgacgac cacgccttaa 1530

<210> 142

<211> 1530

<212> DNA

<213> Serratia marcescens 2880STDY5682930

<400> 142

atggcagaaa gtaatgcggc aattcaatcg gctgcgatta tcggcgcggg aacgatgggc 60

agaggcatcg cttatctttt cgcgcaaaaa ggcattcgca cggtgcttta taatcgcaac 120

ggcaataccc tcaatcaggc tcgcgaatat atcgcgcaag acctgaacaa gaaagtcgaa 180

cagggcaaga tcgcgctgca ggataaaggc gcagtgctgg ccaatctggt gttcacctcg 240

gtgtttgaga ctatcgccga cagcgatctg gtgatagaaa ccatcgccga gcaagagcaa 300

accaaacttg aggtgctggc ggccatcgcc gcggcggtca agcccgacac gctgatcgcc 360

accaatacat cctcactgtc gcttaacaag ctggcaaccg cggtgacgca cagcgagcgg 420

tttatcggtt tgcacttttt caatcccgcg ccgctgatga aactgattga aatcatcccg 480

gcctacttta ccgcacgcgc caccaccgaa cgttgccgtc agctggtggc cgcgttgggg 540

aaacgcgatg tcgtctgcca ggccacgccg gggtttatcg tcaaccgcat ggcccgcccc 600

tactacctgg aagggttccg cctgttggaa gaacacgtgg cgcgcgcgcc gcagatcgac 660

cgcgccctca aggccggcgg gcactttcgc atggggccgc tcgagctgac cgattttatc 720

ggccaggaca tcaactatca ggtcagccgg caaatctggc aggacatgca atacgatccg 780

cgctataccc ccggccatct gcagcgttcg ctggtcgatg ccggtctgtt ggggaaaaag 840

aacggccgct cctattttgc cgccgaagaa aacgccttac cggtaacggc cgccaccgat 900

gcagacgtcg agacgctgcg cgtttacggc gaacaccctt tttttaccct gttgcaacag 960

cgggccgcgc ttcagtggcc acggctgcgc gtggagcaac ggccggcctt accgggcctg 1020

gggccggccg tccagatcaa tgaggctttc accgtcagcg tcaccgatgg ccgcacggcg 1080

agccagttgg cggagctgac ggcggcggat gcctttgtgg tcgatgtcgc cctgaactac 1140

gccgacacgg cgtatctggt ggcagcgcac aaccgccacg cctctgcggc caataaggcg 1200

ctgtttttac gcctgctgca cacggcaatc ccgcaggttg aatttatcaa ggactccccg 1260

gcgctgatcg tcgcccgcgt cctcagcagc ctgatcaacg agtcggtgat catggtggaa 1320

agcggcgtct gcagccggga agacatcaat gtcgccgccg tcgccggcgt taactacgcc 1380

gacggcattt tcggcttgct cgatagcctg ggggagaaaa acgtcaggac gacgctgagc 1440

aacctggcgc agctgctgca cgcggcacgc tatgcgccgc attacaccct tctacacgcc 1500

gcgcaaccgg tgctgacgac cacgccttaa 1530

<210> 143

<211> 1530

<212> DNA

<213> Serratia marcescens 790

<400> 143

atggcagaaa gtaatgcggc aattcaatcg gctgcgatta tcggcgcggg aacgatgggc 60

agaggcatcg cttatctttt cgcgcaaaaa ggcattcgca cggtgcttta taatcgcaac 120

ggcaataccc tcaatcaggc tcgcgaatat atcgcgcaag acctgaacaa gaaagtcgaa 180

cagggcaaga tcgcgctgcg ggataaaggt gcagtgctag ccaatctggt gttcacctcg 240

gtgtttgaga ctatcgccga cagcgagctg gtgatagaaa ccatcgccga gcaagagcaa 300

accaaacttg aggtgctggc ggccatcgcc gcggcggtca agcccgacac gctgatcgcc 360

accaatacct cctcactgtc gcttaacaag ctggcaaccg cggtgacgca cagcgagcgg 420

tttatcggtt tgcacttttt caatcccgcg ccgctgatga aactgattga aatcatcccg 480

gcctacttta ccgcacgcgc caccaccgaa cgttgccgtc agctggtagc cgcgttgggg 540

aaacgcgatg tcgtctgcca ggccacgccg gggtttatcg tcaaccgcat ggcccgcccc 600

tactacctgg aagggttccg cctgttggaa gaacacgtgg cgcgcgcgcc gcagatcgac 660

cgcgccctca aggccggcgg gcactttcgc atggggccgc tcgagctgac cgattttatc 720

ggccaggaca tcaactatca ggtcagccgg caaatctggc aggacatgca atacgatccg 780

cgctataccc ccggccatct gcagcgttcg ctggtcgatg ccggtctgtt ggggaaaaag 840

aacggccgct cctattttgc cgccgaagaa aacgccttac cggtaacggc cgccaccgat 900

gcagacgtcg agacgctgcg cgtttacggc gaacaccctt tttttaccct gttgcaacag 960

cgggccgcgc ttcagtggcc acggctgcgc gtggagcaac ggccggcctt accgggcctg 1020

gggccggccg tccagaccaa tgaggctttc accgtcagcg tcaccgatgg ccgcacggcg 1080

aaccagttgg cggagctgac ggcggcggat gcctttgtgg tcgatgtcgc cctgaactac 1140

gccgacacgg cgtatctggt ggcagcgcac aaccgccacg cctctgcggc caataaggcg 1200

ctgtttttac gcctgctgca cacggcaatc ccgcaggttg aatttatcaa ggactccccg 1260

gcgctgatcg tcgcccgcgt cctcagcagc ctgatcaacg agtcggtgat catggtggaa 1320

agcggcgtct gcagccggga agacatcgat gtcgccgccg tcgctggcgt taactacgcc 1380

gacggcattt tcggctggct cgatagcctg ggggagaaaa acgtcaggac gacgctgagc 1440

aacatggcgc agctgctgca cgcggcacgc tatgcgccgc attacaccct tctgcacgcc 1500

gcgcaaccgg cgctgatgac cacgccttaa 1530

<210> 144

<211> 1530

<212> DNA

<213> Serratia marcescens UMH5

<400> 144

atggcagaaa gtaatgcggc aattcagtcg gctgcgatta tcggcgcggg aacgatgggc 60

agaggcatcg cttatctttt cgcgcaaaaa ggcattcgca cggtgcttta taatcgcaac 120

ggcaataccc tcagtcaggc tcgcgactat atcgagctag acctgaacaa gaaagtcgaa 180

cagggcaaga tcgcgctgca ggataaaggc gcggtgttgg ccaatctggt gttcacctcg 240

gtatttgaga ccattgccga cagcgagctg gtgatagaaa ccatcgccga gcaagagcaa 300

accaaacttg aggtgctggc gaccatcgcc gctgcagtca agcccgacac gctgatcgcc 360

accaatacct cctcactgtc gctaaataag ctggcaaccg cggtgacgca cagcgagcgg 420

tttatcggtt tgcacttttt caaccccgcg ccgctgatga aactgattga aatcatcccg 480

gcctactgta ccgcacacgc caccacggag cgttgccgcc agctggtggc cgcgttgggg 540

aaacgtgacg tcgtctgcca ggccacgccg gggtttatcg tcaaccgcat ggcccgcccc 600

tactacctgg aagggttccg cctattggaa gaacacgtgg cgcgcgcgcc gcagatcgac 660

cgtgccctca aggccggtgg gcactttcgt atggggccac tcgaactgac cgattttatc 720

ggccaggaca tcaactatca ggtcagtcgg caaatctggc aggacatgca atacgacgcg 780

cgctataccc ccggccacct gcagcgttcg ctggtcgatg ccggtctatt gggaaaaaag 840

aacggccgct cctattttgc cgtcgaagaa aacgccccgc cggtgatggc cgccaccgat 900

gcagacattg agacgctgca cgtttacggc gaacaccctt tttttaccct gttacaacag 960

cgggccgcgc ttcagtggcc acggctgcgc gtggaacaac ggcaggcatt accgggcctg 1020

gggccggccg tccggatcaa tgacgctttc accatcagca tcaccgatgg ccgcacggca 1080

aaccagctgg ccgagcagac ggcggcggat gcctttgtgg tcgatgttgc cctgaattac 1140

gccgacacgg cgtatctggt ggcagcgcac agccgccacg cctctggggc caataaagcg 1200

ctgttcttac gcctgctgca cacggcaatc ccgcaggttg aatttatcaa ggattccccg 1260

gccttgatcg tcgcccgcgt cctcagcagc ctaatcaacg agtcggtgat catggtggaa 1320

agcggcgtct gcagccggga agacatcgat gtcgccgccg tcgccggcgt taactacgcc 1380

gacggcattt tcggctggct cgatagcctg ggggagaaaa acgtcaggac gacgctgagc 1440

aacctggcgc agttgctgca cgcggcgcgc tatgcgccgc attacaccct tctgcacgcc 1500

gcgcaaccgg cgctgacgac cacgccttaa 1530

<210> 145

<211> 1530

<212> DNA

<213> Serratia marcescens 2880STDY5682988

<400> 145

atggcagaaa gtaatgcggc aattcagtcg gctgcgatta tcggcgcggg aacgatgggc 60

agaggcatcg cttatctttt cgcgcaaaaa ggcattcgca cggtgcttta taatcgcaac 120

ggcaataccc tcagtcaggc tcgcgactat atcgagctag acctgaacaa gaaagtcgaa 180

cagggcaaga tcgcgctgca ggataaaggc gcggtgttgg ccaatctggt gttcacctcg 240

gtatttgaga ccattgccga cagcgagctg gtgatagaaa ccatcgccga gcaagagcaa 300

accaaacttg aggtgctggc gaccatcgcc gctgcagtca agcccgacac gctgatcgcc 360

accaatacct cctcactgtc gctaaataag ctggcaaccg cggtgacgca cagcgagcgg 420

tttatcggtt tgcacttttt caaccccgcg ccgctgatga aactgattga aatcatcccg 480

gcctactgta ccgcacacgc caccacggag cgttgccgcc agctggtggc cgcgttgggg 540

aaacgtgacg tcgtctgcca ggccacgccg gggtttatcg tcaaccgcat ggcccgcccc 600

tactacctgg aagggttccg cctattggaa gaacacgtgg cgcgcgcgcc gcagatcgac 660

cgtgccctca aggccggtgg gcactttcgt atggggccac tcgaactgac cgattttatc 720

ggccaggaca tcaactatca ggtcagtcgg caaatctggc aggacatgca atacgacgcg 780

cgctataccc ccggccacct gcagcgttcg ctggtcgatg ccggtctatt gggaaaaaag 840

aacggccgct cctattttgc cgtcgaagaa aacgccccgc cggtgatggc cgccaccgat 900

gcagacattg agacgctgca cgtttacggc gaacaccctt tttttaccct gttacaacag 960

cgggccgcgc ttcagtggcc acggctgcgc gtggaacaac ggcaggcatt accgggcctg 1020

gggccggccg tccggatcaa tgacgctttc accatcagca tcaccgatgg ccgcacggca 1080

aaccagctgg ccgagcagac ggcgacggat gcctttgtgg tcgatgttgc cctgaattac 1140

gccgacacgg cgtatctggt ggcagcgcac agccgccacg cctctggggc caataaagcg 1200

ctgttcttac gcctgctgca cacggcaatc ccgcaggttg aatttatcaa ggattccccg 1260

gccttgatcg tcgcccgcgt cctcagcagc ctaatcaacg agtcggtgat catggtggaa 1320

agcggcgtct gcagccggga agacatcgat gtcgccgccg tcgccggcgt taactacgcc 1380

gacggcattt tcggctggct cgatagcctg ggggagaaaa acgtcaggac gacgctgagc 1440

aacctggcgc agttgctgca cgcggcgcgc tatgcgccgc attacaccct tctgcacgcc 1500

gcgcaaccgg cgctgacgac cacgccttaa 1530

<210> 146

<211> 1530

<212> DNA

<213> Serratia marcescens 945154301

<400> 146

atggcagaaa gtaatgcggc aattcagtcg gctgcgatta tcggcgcggg aacgatgggc 60

agaggcatcg cttatctttt cgcgcaaaaa ggcattcgca cggtgcttta taatcgcaac 120

ggcaataccc tcaatcaggc tcgcgactat atcgagctag acctgaacaa gaaagtcgaa 180

cagggcaaga tcgcgctgca ggataaaggc gcggtgttgg ccaatctggt gttcacctcg 240

gtatttgaga ccattgccga cagcgagctg gtgatagaaa ccatcgccga gcaagagcaa 300

accaaacttg aggtgctggc ggccatagcc gcggcagtca agcccgacac gctgatcgcc 360

accaatacct cctcactgtc gctgaataag ctggcaaccg cggtgacgca cagcgagcgg 420

tttatcggtt tgcacttttt taaccccgcg ccgctgatga aactgattga aatcatcccg 480

gcctacttta ccgcacacgc caccacggag cgttgccgtc aactggtggc cgcgttgggg 540

aaacgcgatg ttgtctgcca ggccacgccg gggtttatcg tcaaccgcat ggcccgcccc 600

tactacctgg aagggttccg cctattggaa gaacacgtgg cgcgcgcgcc gcagatcgac 660

cgtgccctca aggctggcgg gcactttcgt atggggccgc tcgagctgac cgattttatc 720

ggccaggaca tcaactatca ggtcagtcgg caaatctggc aggacatgca atacgacgcg 780

cgctataccc ccggccacct gcagcgttcg ctggtcgatg ccggtctatt gggaaaaaag 840

aacggccgct cctattttgc cgccgaagaa aacgccccgc cggtgatggc cgccaccgat 900

gcagacattg agacgctgca cgtttacggc gaacaccctt tttttaccct gttacaacaa 960

cgtgccgcgc ttcagtggcc acagctgcgc gtggaacaac ggccggcatt accgggcctg 1020

gggccggccg tccggatcaa tgacgctttc agcgtcagca tcaccgatgg ccgcacggca 1080

aaccagctgg ccgagcagac ggcggcggat gcctttgtgg tcgatgtcgc cctgaattac 1140

gccgacacgg cgtatctggt ggcagcgcac agccgccatg cctctgcagc caataaagcg 1200

ctgttcttac gcctgctgca cacggcaatc ccgcaggttg aatttatcaa ggattccccg 1260

gcgctgatcg tcgcccgcgt cctcagcagc ctaatcaacg agtcggtgat catggtggaa 1320

agcggcgtct gcagccggga agacatcgat gtcgccgccg tcgccggcgt taactacgcc 1380

gacggcattt tcggctggct agatagcctg ggggagaaaa acgtcaggac gacgctgagc 1440

aacctggcgc agctgctgca cgcagcacgc tatgcgccgc attacaccct tctgcacgcc 1500

gcgcaaccgg cgctgacgac cacgccttaa 1530

<210> 147

<211> 1530

<212> DNA

<213> Serratia marcescens at10508

<400> 147

atggcagaaa gtaatgcggc aattcagtcg gctgcgatta tcggcgcggg aacgatgggc 60

agaggcatcg cttatctttt cgcgcaaaaa ggcattcgca cggtgcttta taatcgcaac 120

ggcaataccc tcaatcaggc tcgcgactat atcgagctag acctgaacaa gaaagtcgaa 180

cagggcaaga tcgcgctgca ggataaaggc gcggtgttgg ccaatctggt gttcacctcg 240

gtatttgaga ccattgccga cagcgagctg gtgatagaaa ccatcgccga gcaagagcaa 300

accaaacttg aggtgctggc ggccatcgcc gcggcagtca agcccgacac gctgatcgcc 360

accaatacct cctcactgtc gctgaataag ctggcaaccg cggtgacgca cagcgagcgg 420

tttatcggtt tgcacttttt taaccccgcg ccgctgatga aactgattga aatcatcccg 480

gcctacttta ccgcacacgc caccacggag cgttgccgtc aactggtgac cgcgttgggg 540

aaacgcgatg ttgtctgcca ggccacgccg gggtttatcg tcaaccgcat ggcccgcccc 600

tactacctgg aagggttccg cctattggaa gaacacgtgg cgcgcgcgcc gcagatcgac 660

cgtgccctca aggccggcgg gcactttcgt atggggccgc tcgagctgac cgattttatc 720

ggccaggaca tcaactatca ggtcagtcgg caaatctggc aggacatgca atacgacgcg 780

cgctataccc ccggccacct gcagcgttcg ctggtcgatg ccggtctatt gggaaaaaag 840

aacggccgct cctattttgc cgtcgaagaa aacgccccgc cggtgatggc cgccaccgat 900

gcagacattg agacgctgca cgtttacggc gaacaccctt tttttaccct gttacaacaa 960

cgtgccgcgc ttcagtggcc acagctgcgc gtggaacaac ggccggcatt accgggcctg 1020

gggccggccg tccggatcaa tgacgctttc accgtcagca tcaccgatgg ccgcacggca 1080

aaccagctgg ccgagcagac ggcggcggat gcctttgtgg tcgatgtcgc cctgaattac 1140

gccgacacgg cgtatctggt ggcagcgcac agccgccatg cctctgcagc caataaagcg 1200

ctgttcttac gcctgctgca cacggcaatc ccgcaggttg aatttatcaa ggattccccg 1260

gcgctgatcg tcgcccgcgt cctcagcagc ctaatcaacg agtcggtgat catggtggaa 1320

agcggcgtct gcagccggga agacatcgat gtcgccgccg tcgccggcgt taactacgcc 1380

gacggcattt tcggctggct agatagcctg ggggagaaaa acgtcaggac gacgctgagc 1440

aacctggcgc agctgctgca cgcagcacgc tatgcgccgc attacaccct tctgcacgcc 1500

gcgcaaccgg cgctgacgac cacgccttaa 1530

<210> 148

<211> 1530

<212> DNA

<213> Serratia marcescens ML2637

<400> 148

atggcagaaa gtaatgcggc aattcagtcg gctgcgatta tcggcgcggg aacgatgggc 60

agaggcatcg cttatctttt cgcgcaaaaa ggcattcgca cggtgcttta taatcgcaac 120

ggcaataccc tcaatcaggc tcgcgactat atcgagctag acctgaacaa gaaagtcgaa 180

cagggcaaga tcgcgctgca ggataaaggc gcggtgttgg ccaatctggt gttcacctcg 240

gtatttgaga ccattgccga cagcgagctg gtgatagaaa ccatcgccga gcaagagcaa 300

accaaacttg aggtgctggc ggccatcgcc gcggcagtca agcccgacac gctgatcgcc 360

accaatacct cctcactgtc gctgaataag ctggcaaccg cggtgacgca cagcgagcgg 420

tttatcggtt tgcacttttt taaccccgcg ccgctgatga aactgattga aatcatcccg 480

gcctacttta ccgcacacgc caccacggag cgttgccgtc aactggtgac cgcgttgggg 540

aaacgcgatg ttgtctgcca ggccacgccg gggtttatcg tcaaccgcat ggcccgcccc 600

tactacctgg aagggttccg cctattggaa gaacacgtgg cgcgcgcgcc gcagatcgac 660

cgtgccctca aggccggcgg gcactttcgt atggggccgc tcgagctgac cgattttatc 720

ggccaggaca tcaactatca ggtcagtcgg caaatctggc aggacatgca atacgacgcg 780

cgctataccc ccggccacct acagcgttcg ctggtcgatg ccggtctatt gggaaaaaag 840

aacggccgct cctattttgc cgtcgaagaa aacgccccgc cggtgatggc cgccaccgat 900

gcagacattg agacgctgca cgtttacggc gaacaccctt tttttaccct gttacaacaa 960

cgtgccgcgc ttcagtggcc acagctgcgc gtggaacaac ggccggcatt accgggcctg 1020

gggccggccg tccggatcaa tgacgctttc accgtcagca tcaccgatgg ccgcacggca 1080

aaccagctgg ccgagcagac ggcggcggat gcctttgtgg tcgatgtcgc cctgaattac 1140

gccgacacgg cgtatctggt ggcagcgcac agccgccacg cctctgcagc caataaagcg 1200

ctgttcttac gcctgctgca cacggcaatc ccgcaggttg aatttatcaa ggattccccg 1260

gcgctgatcg tcgcccgcgt cctcagcagc ctaatcaacg agtcggtgat catggtggaa 1320

agcggcgtct gcagccggga agacatcgat gtcgccgccg tcgccggcgt taactacgcc 1380

gacggcattt tcggctggct agatagcctg ggggagaaaa acgtcaggac gacgctgagc 1440

aacctggcgc agctgctgca cgcagcacgc tatgcgccgc attacaccct tctgcacgcc 1500

gtgcaaccgg cgctgacgac cacgccttaa 1530

<210> 149

<211> 1530

<212> DNA

<213> Serratia marcescens SM1978

<400> 149

atggcagaaa gtaatgcggc aattcagtcg gctgcgatta tcggcgcggg aacgatgggc 60

agaggcatcg cttatctttt cgcgcaaaaa ggcattcgca cggtgcttta taatcgcaac 120

ggcaataccc tcaatcaggc tcgcgactat atcgagctag acctgaacaa gaaagtcgaa 180

cagggcaaga tcgcgctgca ggataaaggc gcggtgttgg ccaatctggt gttcacctcg 240

gtatttgaga ccattgccga cagcgagctg gtgatagaaa ccatcgccga gcaagagcaa 300

accaaacttg aggtgctggc ggccatagcc gcggcagtca agcccgacac gctgatcgcc 360

accaatacct cctcactgtc gctgaataag ctggcaaccg cggtgacgca cagcgagcgg 420

tttatcggtt tgcacttttt taaccccgcg ccgctgatga aactgattga aatcatcccg 480

gcctacttta ccgcacacgc caccacggag cgttgccgtc aactggtgac cgcgttgggg 540

aaacgcgatg ttgtctgcca ggccacgccg gggtttatcg tcaaccgcat ggcccgcccc 600

tactacctgg aagggttccg cctattggaa gaacacgtgg cgcgcgcgcc gcagatcgac 660

cgtgccctca aggccggcgg gcactttcgt atggggccgc tcgagctgac cgattttatc 720

ggccaggaca tcaactatca ggtcagtcgg caaatctggc aggacatgca atacgacgcg 780

cgctataccc ccggccacct gcagcgttcg ctggtcgatg ccggtctatt gggaaaaaag 840

aacggccgct cctattttgc cgccgaagaa aacgccccgc cggtgatggc ctccaccgat 900

gcagacattg agacgctgca cgtttacggc gaacaccctt tttttaccct gttacaacaa 960

cgtgccgcgc ttcagtggcc acagctgcgc gtggaacaac ggccggcatt accgggcctg 1020

gggccggccg tccggatcaa tgacgctttc agcgtcagca tcaccgatgg ccgcacggca 1080

aaccagctgg ccgagcagac gacggcggat gcctttgtgg tcgatgtcgc cctgaattac 1140

gccgacacgg cgtatctggt ggcagcgcac agccgccacg cctctgcagc caataaagcg 1200

ctgttcttac gcctgctgca cacggcaatc ccgcaggttg aatttatcaa ggattccccg 1260

gcgctgatcg tcgcccgcgt cctcagcagc ctaatcaacg agtcggtgat catggtggaa 1320

agcggcgtct gcagccggga agacatcgat gtcgccgccg tcgccggcgt taactacgcc 1380

gacggcattt tcggctggct agatagcctg ggggagaaaa acgtcaggac gacgctgagc 1440

aacctggcgc agctgctgca cgcagcacgc tatgcgccgc attacaccct tctgcacgcc 1500

gcgcaaccgg cgctgacgac cacgccttaa 1530

<210> 150

<211> 1530

<212> DNA

<213> Serratia marcescens PWN146

<400> 150

atggcagaaa gtaatgcggc aattcagtcg gctgcgatta tcggcgcggg aacgatgggc 60

agaggcatcg cttatctttt cgcgcaaaaa ggcattcgca cggtgcttta taatcgcaac 120

ggcaataccc tcaatcaggc tcgcgactat atcgagctag acctgaacaa gaaagtcgaa 180

cagggcaaga tcgcgctaca ggataaaggc gcggtgttgg ccaatctggt gttcacctcg 240

gtatttgaga ccattgccga cagcgagctg gtgatagaaa ccatcgccga gcaagagcaa 300

accaaacttg aggtgctggc gaccatcgcc gcggcagtca agcccgacac gctgatcgcc 360

accaatacct cctcactgtc gctaaataag ctggcaaccg cggtgacgca cagcgagcgg 420

tttatcggtt tgcacttttt taaccccgcg ccgctgatga aactgattga aatcatcccg 480

gcctacttta ccgcacacgc caccacggag cgttgccgtc aactggtggc cgcgttggga 540

aaacgcgacg ttgtctgcca ggccacgccg gggtttatcg tcaaccgcat ggcccgcccc 600

tactacctgg aagggttccg cctattggaa gaacacgtgg cgcgcgcgcc gcagatcgac 660

cgtgccctca aggccggcgg gcactttcgt atggggccgc tcgaactgac cgattttatc 720

ggccaggaca tcaactatca ggtcagccgg caaatctggc aggacatgca atacgacgcg 780

cgctataccc ccggccacct gcagcgttcg ctggtcgatg ccggtctatt gggaaaaaag 840

aacggccgct cctattttgc cgtcgaagaa aacgccccgc cggtgatggc cgccaccgat 900

gcagacattg agacgctgca cgtctgcggc gaacaccctt tttttactct gttgcaacag 960

cgagccgcac ttcagtggcc acggctgcgc gtggaacaac ggccggcctt gccgggcctg 1020

gggcccgccg tctggatcaa tgacgctttc accgtcagca tcaccgatgg ccgcacggca 1080

aaccagctgg ccgagcagac ggcggcggat gcctttgtgg tcgatgtcgc cctgaattac 1140

gccgacacgg cgtatctggt ggcagcacac agccgccacg cctctgcggc caataaagcg 1200

ctgttcttac gcctgctgca caccgcaatc ccgcaggttg aatttatcaa ggattccccg 1260

gcgctgatcg tcgctcgcgt cctcagcagc ctgatcaatg agtcggtgat catggtggaa 1320

agcggcgtct gcagccggga agacatcgat gtcgccgccg tcgccggcgt taactacgcc 1380

gacggcattt tcggctggct cgatagcctg ggggagaaaa acgtcagggc gacgctgagc 1440

aacttggcgc agctgctgca cgcggcgcgc tatgcgccgc attacaccct tctgcacgcc 1500

gcgcaaccgg cgctgacgac cacgccttaa 1530

<210> 151

<211> 1530

<212> DNA

<213> Serratia marcescens H1q

<400> 151

atggcagaac gtaatgcggc aattcaatcg gctgcgatta tcggcgcggg aacgatgggc 60

agaggcatcg cttatctttt cgcgcaaaaa ggcattcgta cggtgcttta taatcgcaac 120

ggcaataccc tcattcaggc tcgtgaatat atcgtgcaag acctggacaa aaaggtcgaa 180

cagggcaggc tcgcgccgca ggataaagac gcggtgctgg ccaatctgca gttctcatcg 240

gtgtttgaag ctatcgtcga cagcgatttg gtgctcgaaa ctatcgccga gcaagagcaa 300

gccaaactcg aggtgctggc cgccatcgct gcggcggtca aacccgacac gctgatcgcc 360

accaatacgt cctcattgtc gctcaataag ctggcaacgg cggtgaccca cagcgaacgc 420

tttatcggtt tgcacttttt caaccccgca ccgctaatga agctgattga aatcattccg 480

gcctacttta ccgcccaagc caccactgaa acttgccgcc aactggtggc ggcattgggg 540

aaacgcgatg tcgtctgcca ggctacgccg gggttcatcg tcaaccgtat ggcccgcccc 600

tactacctgg aaggctttcg cctgttggaa gagcacgtgg cgcgcgcgcc gcagatcgac 660

cgcgccctca aggccggcgg acactttcgc atggggccgc tcgagctgac cgattttatc 720

ggccaggaca tcaactatca ggtcagccgg caaatttggc aggacatgca gtacgacccg 780

cgctataccc ccggccatct gcagcgttcg ctggtcgatg ccgggctgct ggggaaaaag 840

aacggtcgct cctatttttc cgccgaagaa accgccccgc cggttgaggc cgccgtcgag 900

gcggatgtcg agacgctgcg catttacggc gaacaccctt tgtttaccct gctgcaacag 960

cgagccgcac tgcaatggcc gcagttgcgc gtggaacagc ggccggcctt gtcgggcctg 1020

ggagcggcca ttcaggtcaa tgacgctttc accgtcagca tcaccgatgg ccgcacggca 1080

aatcagttgg ccgagcagac ggcggcggac gcctttgtgg tcgatgtcgc cctgaactac 1140

gccgacacgg cgtatctggt ggcggcgcac agccgccacg cctctgcggc taataaggcg 1200

ttgtttttgc gcctgctgca caccgcgctt ccgcaggttg aatttatcaa ggactccccg 1260

gcgctgatcg tcgcccgcgt cctcagcagc ctgatcaacg agtcggtgat catggtggaa 1320

agcggcgtct gcagccggga agacatcgat gtcgccgccg tcgccggcgt gaactacgcc 1380

gacggcattt tcggctggct cactcgcctg ggggagaaaa acgtcaggac gacgctgagc 1440

aacctggcgc agctgctgca cgcggcccgc tatgcgccgc attacaccct tctgcacgcc 1500

gcccaaccgg cgctgaccac cacgccttaa 1530

<210> 152

<211> 1530

<212> DNA

<213> Serratia marcescens UMH6

<400> 152

atggcagaaa gtaatgcggc aattcaatcg gctgcgatta tcggcgcggg aacgatgggc 60

agaggcatcg cttatctttt tgcgcaaaaa agcattcgta cggtgcttta taatcgcaac 120

ggcaataccc tcaatcaagc tcgcgacgct atcgtgcaag acctgaacaa aaaggtggaa 180

cagggcaagc tcgcgctgca ggacaaagac gcggtgctgg ctaatctgac gttctccgcg 240

gaatttgggg ccatcgccga cagcgatctg gtgatcgaaa ccatcgcaga gcatgagcaa 300

gccaaactcg aagtgctggc ggccatcgcc gcgacggtca agcccgacac gctgatcgcc 360

accaatacct cctcactgtc gcttaataag ctggcgacag cggtgacgca cagcgaacgc 420

tttatcggtt tgcacttttt caaccccgcg ccgctgatga agctgattga aatcattccg 480

gcctatttta ccgcacaggt caccaccgaa cgttgccgtc aactggtggc ggcgttgggg 540

aaacgcgatg tcgtctgcca ggccacgccg ggatttatcg tcaaccgtat ggcccgcccc 600

tactatctgg aagggttccg cctgttggaa gaacacgtgg cgcgtgcgcc gcagatcgac 660

cgcgccctca aagccggcgg acactttcgc atggggccgc tcgagctgac cgattttatc 720

ggtcaggaca tcaactatca ggtcagccgg caaatttggc aggacatgca gtacgatccg 780

cgctataccc cgggccatct gcagcgctcg ctggtcgatg ccggtctgtt gggcaaaaag 840

aacggccgct cctatttctc cgccgaagaa tctcccccgc cgcttgcggc cgccgtcgat 900

gcggaggtcg agacgctacg catttacggt gaacatcctc tctttaccct gctacagcag 960

cgggccgccc tgcaatggcc gcggctgcgc gttgaacaac ggccgacatt accgggcctg 1020

ggcgccgcca ttcaggtcaa tgacgctttc accgtcagcg ttaccgatgg ccgcacggca 1080

aaccagcttg ccgaacagac cgcggcggac gcctttgtcg tcgatgttgc cctgaactac 1140

ggcgatacgg cgtacctggt ggcggcacat agccgccacg cctctgcggc caataaggcg 1200

ctgtttttac gcctgctgca caccgcgatc ccgcaggtgg aatttatcaa ggattccccg 1260

gccctgatcg tcgcccgcgt gctcagcagc ctgatcaacg agtcggtgat catggtggaa 1320

agcggcgtct gcagccggga agacattgat gtcgccgccg tcgccggcgt gaactacgcc 1380

gacggtattt tcggctggct cactcgcctc ggggaggaaa atgtcaggac gacgctgagc 1440

aacctggcgc aattgctgca cgcggcgcgc tatgcgccgc attacaccct tctgcacgcc 1500

gcccaaccgg cgctgaccac cacgccttaa 1530

<210> 153

<211> 1530

<212> DNA

<213> Serratia nematodiphila WCU338

<400> 153

atggcagaaa gtaatgcggc aattcaatcg gctgcgatta tcggcgcggg aacgatgggc 60

agaggcatcg cttatctttt cgcgcaaaaa agcattcgta cggtgcttta taatcgcaac 120

ggcaataccc tcaatcaagc tcgcgacgct atcgtgcaag acctgaacaa aaaggtggaa 180

cagggcaagc tcgcgctgca ggataaagac gcggtgctgg ctaatctgac gttctccgcg 240

gaatttgggg ccatcgccga cagcgatctg gtgatcgaaa ccatcgcaga acatgagcaa 300

gccaaactcg aggtgctggc ggccatcgcc gcgacggtca agcccgacac gctgatcgcc 360

accaatacct cctcactgtc gctgaataag ctggcgacgg cggtgacgca cagcgaacgc 420

tttatcggtt tgcacttttt caaccccgcg ccgctgatga agctgattga aatcattccg 480

gcctatttta ccgcacagat caccaccgaa cgttgccgtc aactggtggc ggcattgggg 540

aaacgcgatg tcgtctgtca ggccacgccg gggtttatcg tcaaccgtat ggctcgcccc 600

tactatctgg aaggattccg cctgttggaa gaacacgtgg cgcgtgcgcc gcagatcgac 660

cgcgccctca aagccggcgg acactttcgc atggggccgc tcgagctgac cgattttatc 720

ggtcaggaca tcaactatca ggtcagccgg caaatttggc aggacatgca gtacgatccg 780

cgctataccc cgggccatct gcagcgctcg ctggtcgatg ccggtttgct ggggaaaaag 840

aacggccgct cctatttttc cgccgaagaa tctcccccgc cgcttgcagc cgccgtcgat 900

gcggaggtcg agacgctacg catttacggt gaacatcctc tctttaccct gctacagcag 960

cgggccgccc tgcaatggcc gcggctgcgc gtggaacagc ggccgacatt accaggcctg 1020

ggcgccgcca ttcaggtcaa tgacgctttc accgtcagcg ttaccgatgg ccgcacggca 1080

aaccagcttg ccgaacagac cgcggcggac gcctttgtcg tcgatgtcgc cctgaactac 1140

ggcgatacgg cgtacctggt ggcggcgcat agccgccacg catctgcggc caataaggcg 1200

ctgtttttac gcctgctgca caccgcgatc ccgcaggtgg aatttatcaa ggattccccg 1260

gccttgatcg tcgcccgcgt gctcagcagc ctgatcaacg agtcggtgat catggtggaa 1320

agcggcgtct gcagccggga agacattgat gtcgccgccg tcgccggcgt gaactacgcc 1380

gacggtattt tcggctggct cactcgcctc ggggaggaaa atgtcaggac gacgctgagc 1440

aacctggcgc aattgctgca cgcggcgcgc tatgcgccgc attacaccct tctgcacgcc 1500

gcccaaccgg cgctgaccac cacgccttaa 1530

<210> 154

<211> 1530

<212> DNA

<213> Serratia sp. OLEL1

<400> 154

atggcagaaa gtaatgcggc aattcaatcg gctgcgatta tcggcgcggg aacgatgggc 60

agaggcatcg cttatctttt ggcgcaaaaa agcattcgta cggtgcttta taatcgcaac 120

ggcaataccc tcaatcaagc tcgcgacgct atcgtgcaag acctgaacaa aaaggtggaa 180

cagggcaagc tcgcgctgca ggacaaagac gcggtgctgg ctaatctgac gttctccgcg 240

gaatttgggg ccatcgccga cagcgatctg gtgatcgaaa ccatcgcaga gcatgagcaa 300

gccaaactcg aagtgctggc ggccatcgcc gcgacggtca agcccgacac gctgatcgcc 360

accaatacct cctcactgtc gcttaataag ctggcgacgg cggtgacgca cagcgaacgc 420

tttatcggtt tgcacttttt caaccccgcg ccgctgatga agctgattga aatcattccg 480

gcctatttta ccgcacaggt caccaccgaa cgttgccgtc aactggtggc ggcgttgggg 540

aaacgcgatg tcgtctgcca ggccacgccg ggatttatcg tcaaccgtat ggcccgcccc 600

tactatctgg aagggttccg cctgttggaa gaacacgtgg cgcgtgcgcc gcagatcgac 660

cgcgccctca aagccggcgg acactttcgc atggggccgc tcgagctgac cgattttatc 720

ggtcaggaca tcaactatca ggtcagccgg caaatttggc aggacatgca gtacgatccg 780

cgctataccc cgggccatct gcagcgctcg ctggtcgatg ccggtctgtt gggcaaaaag 840

aacggccgct cctatttctc cgccgaagaa tctcccccgc cgcttgcggc cgccgtcgat 900

gcggaggtcg agacgctacg catttacggt gaacatcctc tctttaccct gctacagcag 960

cgggccgccc tgcaatggcc gcggctgcgc gtggaacaac ggccgacatt accgggcctg 1020

ggcgccgcca ttcaggttaa tgacgctttc accgtcagcg ttaccgatgg ccgcacggca 1080

aaccagcttg ccgaacagac tgcggcggac gcctttgtcg tcgatgtcgc cctgaactac 1140

ggcgatacgg cgtacctggt ggcggcgcat agccgccacg cctctgcggc caataaggcg 1200

ctgtttttac gcctgctgca caccgcgatc ccgcaggtgg aatttatcaa ggattccccg 1260

gccttgatcg tcgcccgcgt gctcagcagc ctgatcaacg agtcggtgat catggtggaa 1320

agcggcgtct gcagccggga agacattgat gtcgccgccg tcgccggcgt gaactacgcc 1380

gacggcattt tcggctggct cactcgcctc ggggaggaaa atgtcaggac gacgctgagc 1440

aacctggcgc aattgctgca cgcggcgcgc tatgcgccgc attacaccct tctgcacgcc 1500

gcccaaccgg cgctgaccac cacgccttaa 1530

<210> 155

<211> 1530

<212> DNA

<213> Serratia marcescens 7209

<400> 155

atggcagaaa gtaatgcggc aattcaatcg gctgcgatta tcggcgcggg aacgatgggc 60

agaggcatcg cttatctttt tgcgcaaaaa agcattcgta cggtgcttta taatcgcaac 120

ggcaataccc tcaatcaagc tcgcgacgct atcgtgcaag acctgaacaa aaaggtggaa 180

cagggcaagc tcgcgctgca ggacaaagac gcggtgctgg ctaatctgac gttctccgca 240

gaatttgggg tcatcgccga cagcgatctg gtgatcgaaa ccatcgcaga acatgagcaa 300

gccaaactcg aggtgctggc ggccatcgcc gcgacggtca agcccgacac gctgatcgcc 360

accaatacct cctcactgtc gcttaataag ctggcgacgg cggtgacgca cagcgaacgc 420

tttatcggtt tgcacttttt caaccccgcg ccgctgatga agctgattga aatcattccg 480

gcctatttta ccgcacaggt caccaccgaa cgttgccgtc aactggtggc ggcgttaggg 540

aaacgcgatg tcgtctgtca ggccacgccg ggatttatcg tcaaccgtat ggcccgcccc 600

tactatctgg aagggttccg cctgttggaa gaacacgtgg cgcgtgcgcc gcagatcgac 660

cgcgccctca aagccggcgg acactttcgc atggggccgc tcgagctgac cgattttatc 720

ggtcaggaca tcaactatca ggtcagccgg caaatttggc aggacatgca gtacgatccg 780

cgctataccc cgggccatct gcagcgctcg ctggtcgatg ccggtctgtt gggcaaaaag 840

aacggccgct cctatttttc cgccgaagaa tctcccccgc cgcttgcggc cgccgtcgat 900

gcggaagtcg agacgctacg catttacggt gaacatcctc tctttaccct gctacagcag 960

cgggccgccc tgcaatggcc gcggctgcgc gtggaacaac ggccgacatt accgggcctg 1020

ggcgccgcca ttcaggtcaa tgacgctttc accgtcagcg ttaccgatgg ccgcacggca 1080

aaccagcttg ccgaacagac cgcggcggac gcctttgtcg tcgatgtcgc cctgaactac 1140

ggcgatacgg cgtacctggt ggcggcacat agccgccatg cctctgcggc caataaggcg 1200

ctgtttttac gcctgctgca caccgcgatc ccgcaggtgg aatttatcaa ggattccccg 1260

gccttgatcg tcgcccgcgt gctcagcagc ctgatcaacg agtcggttat catggtggaa 1320

agcggcgtct gcagccggga agacattgat gtcgccgccg tcgccggcgt gaactacgcc 1380

gacggtattt tcggctggct cactcgcctc ggggaggaaa atgtcaggac gacgctgagc 1440

aacctggcgc aattgctgca cgcggcgcgc tatgcgccgc attacaccct tctgcacgcc 1500

gcccaaccgg cgctgaccac cacgccttaa 1530

<210> 156

<211> 1530

<212> DNA

<213> Serratia marcescens sicaria (Ss1)

<400> 156

atggcagaaa gtaatgcggc aattcaatcg gctgcgatta tcggcgcggg aacgatgggc 60

agaggcatcg cttatctttt tgcgcaaaaa agcattcgta cggtgcttta taatcgcaac 120

ggcaataccc tcaatcaagc tcgcgacgct atcgtgcaag acctgaacaa aaaggtggaa 180

cagggcaagc tcgcgctgca ggacaaagac gcggtgctgg ctaatctgac gttctccgcg 240

gaatttgggg ccatcgccga cagcgatctg gtgatcgaaa ccatcgcaga gcatgagcaa 300

gccaaactcg aggtgctggc ggccatcgcc gcgacggtca agcccgacac gctgatcgcc 360

accaatacct cctcactgtc gcttaataag ctggcgacgg cggtgacgca cagcgaacgc 420

tttatcggtt tgcacttttt caaccccgcg ccgctgatga agctgattga aatcattccg 480

gcctatttta ccgcacaggt caccaccgaa cgttgccgtc aactggtggc ggcgttgggg 540

aaacgcgatg tcgtctgcca ggccacgccg ggatttatcg tcaaccgtat ggcccgcccc 600

tactatctgg aagggttccg cctgttggaa gaacacgtgg cgcgtgcgcc gcagatcgac 660

cgcgccctca aagccggcgg acactttcgc atggggccgc tcgagctgac cgattttatc 720

ggtcaggaca tcaactatca ggtcagccgg caaatttggc aggacatgca gtacgatccg 780

cgctataccc cgggccatct gcagcgctcg ctggtcgatg ccggtctgtt gggcaaaaag 840

aacggccgct cctatttttc caccgaagaa tctcccccgc cgcttgcggc cgccgtcgat 900

gcggaggtcg agacgctacg catttacggt gaacatcctc tctttaccct gctacagcag 960

cgggccgccc tgcaatggcc gcggctgcgc gtggaacaac ggccgacatt accgggcttg 1020

ggcgccgcca ttcaggtcaa tgacgctttc accgtcagcg ttaccgatgg ccgcacggca 1080

aaccagcttg ccgaacagac cgcggcggac gcctttgtcg tcgatgtcgc cctgaactac 1140

ggcgatacgg cctacctggt ggcggcgcat agccgccacg cctctgcggc caataaggcg 1200

ctgtttttac gcctgctgca caccgcgatc ccgcaggtgg aatttatcaa ggattccccg 1260

gccttgatcg tcgcccgcgt gctcagcagc ctgattaacg agtcggtgat catggtggaa 1320

agcggcgtct gtagccggga agacattgat gtcgccgccg tcgccggcgt gaactacgcc 1380

gacggtattt tcggctggct cactcgcctc ggggaggaaa atgtcaggac gacgctgagc 1440

aacctggcgc aattgctgca cgcggcgcgc tatgcgccgc attacaccct tctgcacgcc 1500

gcccaaccgg cgctgaccac cacgccttaa 1530

<210> 157

<211> 1530

<212> DNA

<213> Serratia sp. OLFL2

<400> 157

atggcagaaa gtaatgcggc aattcaatcg gctgcgatta tcggcgcggg aacgatgggc 60

agaggcatcg cttatctttt ggcgcaaaaa agcattcgta cggtgcttta taatcgcaac 120

ggcaataccc tcaatcaagc tcgcgacgct atcgtgcaag acctgaacaa aaaggtggaa 180

cagggcaagc tcgcgctgca ggacaaagac gcggtgctgg ctaatctgac gttctccgcg 240

gaatttgggg ccatcgccga cagcgatctg gtgatcgaaa ccatcgcaga gcatgagcaa 300

gccaaactcg acgtgctggc ggccatcgcc gcgacggtca agcccgacac gctgatcgcc 360

accaatacct cctcactgtc gcttaataag ctggcgacgg cggtgacgca cagcgaacgc 420

tttatcggtt tgcacttttt caaccccgcg ccgctgatga agctgattga aatcattccg 480

gcctatttta ccgcacaggt caccaccgaa cgttgccgtc aactggtggc ggcgttgggg 540

aaacgcgatg tcgtctgcca ggccacgccg ggatttatcg tcaaccgtat ggcccgcccc 600

tactatctgg aagggttccg cctgttggaa gaacacgtgg cgcgtgcgcc gcagatcgac 660

cgcgccctca aagccggcgg acactttcgc atggggccgc tcgagctgac cgattttatc 720

ggtcaggaca tcaactatca ggtcagccgg caaatttggc aggacatgca gtacgatccg 780

cgctataccc cgggccatct gcagcgctcg ctggtcgatg ccggtctgtt gggcaaaaag 840

aacggccgct cctatttctc cgccgaagaa tctcccccgc cgcttgcggc cgccgtcgat 900

gcggaggtcg agacgctacg catttacggt gaacatcctc tctttaccct gctacagcag 960

cgggccgccc tgcaatggcc gcggctgcgc gtggaacaac ggccgacatt accgggcctg 1020

ggcgccgcca ttcaggttaa tgacgctttc accgtcagcg ttaccgatgg ccgcacggca 1080

aaccagcttg ccgaacagac tgcggcggac gcctttgtcg tcgatgtcgc cctgaactac 1140

ggcgatacgg cgtacctggt ggcggcgcat agccgccacg cctctgcggc caataaggcg 1200

ctgtttttac gcctgctgca caccgcgatc ccgcaggtgg aatttatcaa ggattccccg 1260

gccttgatcg tcgcccgcgt gctcagcagc ctgatcaacg agtcggtgat catggtggaa 1320

agcggcgtct gcagccggga agacattgat gtcgccgccg tcgccggcgt gaactacgcc 1380

gacggcattt tcggctggct cactcgcctc ggggaggaaa atgtcaggac gacgctgagc 1440

aacctggcgc aattgctgca cgcggcgcgc tatgcgccgc attacaccct tctgcacgcc 1500

gcccaaccgg cgctgaccac cacgccttaa 1530

<210> 158

<211> 1530

<212> DNA

<213> Serratia marcescens BIDMC 81

<400> 158

atggcagaaa gtaatgcggc aattcaatcg gctgcgatta tcggcgcggg aacgatgggc 60

agaggcatcg cttatctttt ggcgcaaaaa agcattcgta cggtgcttta taatcgcaac 120

ggcaataccc tcaatcaagc tcgcgacgct atcgtgcaag acctgaacaa aaaggtggaa 180

cagggcaagc tcgcgctgca ggacaaagac gcggtgctgg ctaatctgac gttctccgcg 240

gaatttggag ccatcgccga cagcgatctg gtgatcgaaa ccatcgcaga gcatgagcaa 300

gccaaactcg aggtgctggc ggccatcgcc gcgacggtca agcccgacac gctgatcgcc 360

accaatacct cctcactgtc gcttaataag ctggcgacgg cggtaacgca cagcgaacgc 420

tttatcggtt tgcacttttt caaccccgcg ccgctgatga agctgattga aatcattccg 480

gcctatttta ccgcacaggt caccaccgaa cgttgccgtc aactggtggc ggcgttgggg 540

aaacgcgatg tcgtctgcca ggccacgccg ggatttatcg tcaaccgtat ggcccgcccc 600

tactatctgg aagggttccg cctgttggaa gaacacgtgg cgcgtgcgcc gcagatcgac 660

cgagccctca aagccggcgg acactttcgc atggggccgc tcgagctgac cgattttatc 720

ggtcaggaca tcaactatca ggtcagccgg caaatttggc aggacatgca gtacgatccg 780

cgctataccc cgggccatct gcagcgctcg ctggtcgatg ccggtctgtt gggcaaaaag 840

aacggccgct cctatttttc cgccgaagaa tctcccccgc cgcttgcggc cgccgtcgat 900

gcggaggtcg agacgctacg catttacggt gaacatcctc tctttaccct gctacagcag 960

cgggccgccc tgcaatggcc gcggctgcgc gtggaacaac ggccgacatt accggacctg 1020

ggcgccgcca ttcaggtcaa tgacgctttc accgtcagcg ttaccgatgg ccgcacggca 1080

aaccagcttg ccgaacagac cgcggcggac gcctttgtcg tcgatgtcgc cctgaactac 1140

ggcgatacgg cgtacctggt ggcggcgcat agccgccacg cctctgcggc caataaggcg 1200

ctgtttttac gcctgctgca caccgcgatc ccgcaggtgg aatttatcaa ggattccccg 1260

gccttgatcg tcgcccgcgt gctcagcagc ctgatcaacg agtcggtgat catggtggaa 1320

agcggcgtct gcagccggga agacattgat gtcgccgccg tcgccggcgt gaactacgcc 1380

gacggtattt tcggctggct cactcgcctc ggggaggaaa atgtcaggac gacgctgagc 1440

aacctggcgc aattgctgca cgcggcgcgc tatgcgccgc attacaccct tctgcacgcc 1500

gcccaaccgg cgctgaccac cacgccttaa 1530

<210> 159

<211> 1530

<212> DNA

<213> Serratia marcescens BIDMC 50

<400> 159

atggcagaaa gtaatgcggc aattcaatcg gctgcgatta tcggcgcggg aacgatgggc 60

agaggcatcg cttatctttt tgcgcaaaaa agcattcgta cggtgcttta taatcgcaac 120

ggcaataccc tcaatcaagc tcgcgacgct atcgtgcaag acctgaacaa aaaggtggaa 180

cagggcaagc tcgcgctgca ggacaaagac gtggtgctgg ctaatctgac gttctccgcg 240

gaatttgggg ccatcgccga cagcgatctg gtgatcgaaa ccatcgcaga gcatgagcaa 300

gccaaactcg aggtgctggc ggccatcgcc gcgacggtca agcccgacac gctgatcgcc 360

accaatacct cctcactgtc gcttaataag ctggcgacgg cggtgacgca cagcgaacgc 420

tttatcggtt tgcacttttt caaccccgcg ccgctgatga agctgattga aatcattccg 480

gcctatttta ccgcacaggt caccaccgaa cgttgccgtc aactggtggc ggcgttaggg 540

aaacgcgatg tcgtctgtca ggccacgccg ggatttatcg tcaaccgtat ggcccgcccc 600

tactatctgg aagggttccg cctgttggaa gaacacgtgg cgcgtgcgcc gcagatcgac 660

cgcgccctca aagccggcgg acactttcgc atggggccgc tcgagctgac cgattttatc 720

ggtcaggaca tcaactatca ggtcagccgg caaatttggc aggacatgca gtacgatccg 780

cgctataccc cgggccatct gcagcgctcg ctggtcgatg ccggtctgtt gggcaaaaag 840

aacggccgct cctatttttc cgccgaagaa tctcccccgc cgcttgcggc cgccgtcgat 900

gcggaggtcg agacgctacg catttacggt gaacatcctc tctttaccct gctacagcag 960

cgggccgccc tgcaatggcc gcggctgcgc gtggaacaac ggccgacatt accggacctg 1020

ggcgccgcca ttcaggtcaa tgacgctttc accgtcagcg ttaccgatgg ccgcacggca 1080

aaccagcttg ccgaacagac cgcggcggac gcctttgtcg tcgatgtcgc cctgaactac 1140

ggcgatacgg cgtacctggt ggcggcacat agccgccatg cctctgcggc caataaggcg 1200

ctgtttttac gcctgctgca caccgcgatc ccgcaggtgg aatttatcaa ggattccccg 1260

gccttgatcg tcgcccgcgt gctcagcagc ctgatcaacg agtcggtgat catggtggaa 1320

agcggcgtct gcagccggga agacattgat gtcgccgccg tcgccggcgt gaactacgcc 1380

gacggtattt tcggctggct cactcgcctc ggggaggaaa atgtcaggac gacgctgagc 1440

aacctggcgc aattgctgca cgcggcgcgc tatgcgccgc attacaccct tctgcacgcc 1500

gcccaaccgg cgctgaccac cacgccttaa 1530

<210> 160

<211> 1530

<212> DNA

<213> Serratia marcescens UMH7

<400> 160

atggcagaaa gtaatgcggc aattcaatcg gctgcgatta tcggcgcggg aacgatgggc 60

agaggcatcg cttatctttt ggcgcaaaaa agcattcgta cggtgcttta taatcgcaac 120

ggcaataccc tcaatcaagc tcgcgacgct atcgtgcaag acctgaacaa aaaggtggaa 180

cagggcaagc tcgcgctgca ggacaaagac acggtgctgg ctaatctgac gttctccgcg 240

gaatttgggg ccatcgccga cagcgatctg gtgatcgaaa ccatcgcaga gcatgagcaa 300

gccaaactcg aggtgctggc ggccatcgcc gcgacggtca agcccgacac gctgatcgcc 360

accaatacct cctcactgtc gcttaataag ctggcgacgg cggtgacgca cagcgaacgc 420

tttatcggtt tgcacttttt caaccccgcg ccgctgatga agctgattga aatcattccg 480

gcctatttta ccgcacaggt caccaccgaa cgttgccgtc aactggtggc ggcgttgggg 540

aaacgcgatg tcgtctgtca ggccacgccg ggatttatcg tcaaccgtat ggcccgcccc 600

tactatctgg aagggttccg cctgttggaa gaacacgtgg cgcgtgcgcc gcagatcgac 660

cgcgccctca aagccggcgg acactttcgc atggggccgc tcgaactgac cgattttatc 720

ggtcaggaca tcaactatca ggtcagccgg caaatttggc aggacatgca gtacgatccg 780

cgctataccc cgggccatct gcagcgctcg ctggtcgatg ccggtctgtt gggcaaaaag 840

aacggccgct cctatttttc cgccgaagaa tctcctccgc cgcttgcggc cgccgtcgat 900

gcggaggtcg agacgctacg catttacggt gaacatcctc tctttactct gctacagcag 960

cgggccaccc tgcaatggcc gcggctgcgc gtggaacaac ggccgacatt accgggcctg 1020

ggcgccgcca ttcaggttaa tgacgctttc accgtcagcg ttaccgatgg ccgcacggca 1080

aaccagcttg ccgaacagac cgcggcggac gcctttgtcg tcgatgtcgc cctgaactac 1140

ggcgatacgg cgtacctggt ggcggcgcat agccgccacg cctctgcagc caataaggcg 1200

ctgtttttac gcctgctgca caccgcgatc ccgcaggtgg aatttatcaa ggattccccg 1260

gccttgatcg tcgcccgcgt gctcagcagc ctgatcaacg agtcggtgat catggtggaa 1320

agcggcgtct gcagccggga agacattgat gtcgccgccg tcgccggcgt gaactacgcc 1380

gacggtattt tcggctggct cactcgcctc ggggaggaaa atgtcaggac gacgctgagc 1440

aacctggcgc aattgctgca cgcggcgcgc tatgcgccgc attacaccct tctgcacgcc 1500

gcccaaccgg cgctgaccac cacgccttaa 1530

<210> 161

<211> 1530

<212> DNA

<213> Serratia marcescens RSC-14

<400> 161

atggcagaaa gtaatgcggc aattcaatcg gctgcgatta tcggcgcggg aacgatgggc 60

agaggcatcg cttatctttt ggcgcaaaaa agcattcgta cggtgcttta taatcgcaac 120

ggcaataccc tcaatcaagc tcgcgacgct atcgtgcaag acctgaacaa aaaggtggaa 180

cagggcaagc tcgcgctgca ggacaaagac gcggtgctgg ctaatctgac gttctccacg 240

gaatttgggg ccatcgccga cagcgatctg gtgatcgaaa ccatcgcaga gcatgagcaa 300

gccaaactcg aagtgctggc ggccatcgcc gcgacggtca agcccgacag gctgatcgcc 360

accaatacct cctcactgtc gcttaataag ctggcgacgg cggtgacgca cagcgaacgc 420

tttatcggtt tgcacttttt caaccccgcg ccgctgatga agctgattga aatcattccg 480

gcctatttta ccgcacaggt caccaccgaa cgttgccgtc aactggtggc ggcgttaggg 540

aaacgcgatg tcgtctgtca ggccacgccg ggatttatcg tcaaccgtat ggcccgcccc 600

tactatctgg aagggttccg cctgttggaa gaacacgtgg cgcgtgcgcc gcagatcgac 660

cgcgccctca aagccggcgg acactttcgc atggggccgc tcgagctgac cgattttatc 720

ggtcaggaca tcaactatca ggtcagccgg caaatttggc aggacatgca gtacgatccg 780

cgctataccc cgggccatct gcagcgctcg ctggtcgatg ccggtctgtt gggcaaaaag 840

aacggccgct cctatttttc cgccgaagaa tctcccccgc cgcttgcggc cgccgtcgat 900

gcggaagtcg agacgctacg catttacggt gaacatcctc tctttaccct gctacagcag 960

cgggccgccc tgcaatggcc gcggctgcgc gtggaacaac agccgacatt accgggcctg 1020

ggcgccgcca ttcaggtcaa tgacgctttc accgtcagcg ttaccgatgg ccgcacggca 1080

aaccagcttg ccgaacagac cgcggcggac gcctttgtcg tcgatgtcgc cctgaactac 1140

ggcgatacgg cgtacctggt ggcggcacat agccgccatg cctctgcggc caataaggcg 1200

ctgtttttac gcctgctgca caccgcgatc ccgcaggtgg aatttatcaa ggattccccg 1260

gccttgatcg tcgcccgcgt gctcagcagc ctgatcaacg agtcggtgat catggtggaa 1320

agcggcgtct gcagccggga agacattgat gtcgccgccg tcgccggcgt gaactacgcc 1380

gacggtattt tcggctggct cactcgcctc ggggaggaaa atgtcaggac gacgctgagc 1440

aacctggcgc aattgctgca cgcggcgcgc tatgcgccgc attacaccct tctgcacgcc 1500

gcccaaccgg cgctgaccac cacgccttaa 1530

<210> 162

<211> 1530

<212> DNA

<213> Serratia marcescens SM03

<400> 162

atggcagaac gtaatgcggc aattcaatcg gctgcgatta tcggcgcggg aacgatgggc 60

agaggcatcg cttatctttt cgcgcaaaaa ggcattcgta cggtgcttta taatcgcaac 120

ggcaataccc tcattcaggc tcgtgaatat atcgtgcaag acctggacaa aaaggtcgaa 180

cagggcaggc tcgcgccgca ggataaagac gcggtgctgg ccaatctgca gttctcatcg 240

gtgtttgaag ctatcgtcga cagcgatttg gtgctcgaaa ctatcgccga gcaagagcaa 300

gccaaactcg aggtgctggc cgccatcgct gcggcggtca aacccgacac gctgatcgcc 360

accaatacgt cctcattgtc gctcaataag ctggcaacgg cggtgaccca cagcgaacgc 420

tttatcggtt tgcacttttt caaccccgca ccgctaatga agctgattga aatcattccg 480

gcctacttta ccgcccaagc caccactgaa acttgccgcc aactggtggc ggcattgggg 540

aaacgcgatg tcgtctgcca ggctacgccg gggttcatcg tcaaccgtat ggcccgcccc 600

tactacctgg aaggctttcg cctgttggaa gagcacgtgg cgcgcgcgcc gcagatcgac 660

cgcgccctca aggccggcgg acactttcgc atggggccgc tcgagctgac cgattttatc 720

ggccaggaca tcaactatca ggtcagccgg caaatttggc aggacatgca gtacgacccg 780

cgctataccc ccggccatct gcagcgttcg ctggtcgatg ccgggctgct ggggaaaaag 840

aacggtcgct cctatttttc cgccgaagaa accgccccgc cggttgaggc cgccgccgag 900

gcggatgtcg agacgctgcg catttacggc gaacaccctt tgtttaccct gctgcaacag 960

cgagccgcac tgcaatggcc gcagttgcgc gtggaacagc ggccggcctt gtcgggcctg 1020

ggagcggcca ttcaggtcaa tgacgctttc accgtcagcg tcaccgatgg ccgcacggca 1080

aatcagttgg ccgagcagac ggcggcggac gcctttgtgg tcgatgtcgc cctgaactac 1140

gccgacacgg cgtatctggt ggcggcgcac agccgccacg cctctgcggc taataaggcg 1200

ttgtttttgc gcctgctgca caccgcgctt ccgcaggttg aatttatcaa ggactccccg 1260

gcgctgatcg tcgcccgcgt cctcagcagc ctgatcaacg agtcggtgat catggtggaa 1320

agcggcgtct gcagccggga agacatcgat gtcgccgccg tcgccggcgt gaactacgcc 1380

gacggcattt tcggctggct cactcgcctg ggggagaaaa acgtcaggac gacgctgagc 1440

aacctggcgc agctgctgca cgcggcccgc tatgcgccgc attacaccct tctgcacgcc 1500

gcccaaccgg cgctgaccac cacgccttaa 1530

<210> 163

<211> 1530

<212> DNA

<213> Serratia marcescens 90-166

<400> 163

atggcagaaa gtaatgcggc aattcaatcg gctgcaatta tcggcgcggg aacgatgggc 60

agaggcatcg cttatctttt cgcgcaaaaa ggcattcgta cggtgcttta taatcgcaac 120

ggcaataccc tcattcaggc tcgtgaatat atcgtgcaag acctggacaa aaagatcgaa 180

cagggcaggg tcgcgccgca ggataaagac gcggtgctgg ctaatctgac gttctccgcg 240

gaatttgggg ccatcgtcga cagcgatctg gtgatcgaaa ccattgcaga gcatgagcaa 300

gccaaactcg aggtgctggc ggccatcgcc gcgacggtca agcccgacac gctgatcgcc 360

accaatacct cctcactgtc gctgaataag ctggcgacgg cggtgacgca cagcgaacgc 420

tttatcggtt tgcacttttt caaccccgcg ccgctgatga agctgattga aatcattccg 480

gcctatttta ccgcacaggt caccaccgaa cgttgccgtc aactggtggc ggcattgggg 540

aaacgcgatg tcgtctgtca ggccacgccg gggtttatcg tcaaccgtat ggctcgcccc 600

tactatctgg aagggttccg cctgttggaa gaacacgtgg cacgtgcgcc gcagatcgac 660

cgcgccctca aagccggcgg acactttcgc atggggccgc tcgagctgac cgattttatc 720

ggtcaggaca tcaactatca ggtcagccgg caaatttggc aggacatgca gtacgatccg 780

cgctataccc cgggccatct gcagcgctcg ctggtcgatg ccggtctgtt gggcaaaaag 840

aacggccgct cctatttttc cgccgaagaa tctcccccgc cgcttgcagc cgccgtcgat 900

gcggagatcg agacgctgcg catttacggt gaacatcctc tctttaccct gctacagcag 960

cgggccgccc tgcaatggcc gcggctgcgc gtggaacacc ggccgacatt accgggcctg 1020

ggcgccgcca ttcaggtcaa tgatgctttc accgtcagcg ttaccgatgg tcgcacggca 1080

aaccagcttg ccgaacagac cgcggcggac gcctttgtcg tcgatgtcgc cctgaactac 1140

ggcgatacgg cgtacctggc ggcggcgcat agccgccacg cctctgcggc caataaggcg 1200

ctgtttttac gcctgctgca caccgcgatc ccccaggtgg aatttatcaa ggattccccg 1260

gccttgatcg tcgcccgcgt gctcagcagc ctgatcaacg agtcggtgat catggtggaa 1320

agcggcgtct gcagccggga agacattgat gtcgccgccg tcgccggcgt gaactacgcc 1380

gacggtattt tcgtctggct cactcgcctc ggggaggaaa atgtcaggac gacgctgagc 1440

aacctggcgc aattgctgca cgcggcgcgc tatgcaccgc attacaccct tctgcacgcc 1500

gcccaaccgg cgctgacctc cacgccttaa 1530

<210> 164

<211> 1530

<212> DNA

<213> Serratia marcescens UMH2

<400> 164

atggcagaaa gtaatgcggc aattcaatcg gctgcgatta tcggcgcggg aacgatgggc 60

agaggcatcg cttatctttt ggcgcaaaaa agcattcgta cggtgcttta taatcgcaac 120

ggcaataccc tcaatcaagc tcgcgacgct atcgtgcaag atctgaacaa aaaggtggaa 180

cagggcaagc tcgcgctgca ggacaaagac gcggtgctgg ctaatctgac gttctccacg 240

gaatttgggg ccatcgccga cagcgatctg gtgatcgaaa ccatcgcaga gcatgagcaa 300

gccaaactcg aagtgctggc ggccatcgcc gcgacggtca agcccgacac gctgatcgcc 360

accaatacct cctcactgtc gcttaataag ctggcgacgg cggtgacgca cagcgaacgc 420

tttatcggtt tgcacttttt caaccccgcg ccgctgatga agctgattga aatcattccg 480

gcctatttta ccgcacaggt caccaccgaa cgttgccgtc aactggtggc ggcgttgggg 540

aaacgcgatg tcgtctgtca ggccacgccg ggatttatcg tcaaccgtat ggcccgcccc 600

tactatctgg aagggttccg cctgttggaa gaacacgtgg cgcgtgcgcc gcagatcgac 660

cgcgccctca aagccggcgg acactttcgc atggggccgc tcgagctgac cgattttatc 720

ggtcaggaca tcaactatca ggtcagccgg caaatttggc aggacatgca gtacgatccg 780

cgctataccc cgggccatct gcagcgctcg ctggtcgatg ccggtctgtt gggcaaaaag 840

aacggccgct cctatttttc cgccgaagaa tctcccccgc cgcttgcggc cgccgtcgat 900

gcggaggtcg aggcgctacg catttacggt gaacatcctc tctttaccct gctacagcag 960

cgggccgccc tgcaatggcc gcggctgcgc gtggaacaac ggccgacatt accgggcttg 1020

ggcgccgcca ttcaggtcaa tgacgctttc accgtcagcg ttaccgatgg ccgcacggca 1080

aaccagcttg ccgaacagac cgcggcggac gcctttgtcg tcgatgtcgc cctgaactac 1140

ggcgatacgg cgtacctggt ggcggcgcat agccgccacg cctctgcggc caataaggcg 1200

ctgtttttac gcctgctgca caccgcgatc ccgcaggtgg aatttatcaa ggattccccg 1260

gccctgatcg tcgcccgcgt gctcagcagc ctgatcaacg agtcggtgat catggtggaa 1320

agcggcgtct gcagccggga agacattgat gtcgccgccg tcgccggcgt gaactacgcc 1380

gacggtattt tcggctggct cactcacctc ggggaggaaa atgtcaggac gacgctgagc 1440

aacctggcgc aattactcca cgcggcgcgc tatgcgccgc attacaccct tctgcacgcc 1500

gcccaaccgg cgctgaccac cacgccttaa 1530

<210> 165

<211> 1530

<212> DNA

<213> Serratia plymuthica AS9

<400> 165

atggcagaga ataattcggc aatccgttca gccgccgtta ttggtgcggg aaccatgggc 60

agaggcatcg cctatctcct ggcgctgaac ggcatacgaa ccgtacttta taatcgcaat 120

ggtaataatc tcaatcaggc ccgtgactat attgtcagtg acctggacag aaaaatggat 180

aacggaaaaa taaccctgca gaaaaaaggc cagatattag ccaatattat tttctcggcc 240

gtctttgacg ccataaccga cagcgatctg gtgattgaaa ccattgcgga agatgagcaa 300

accaagcatg aaatcctggc agccattgcg gctacggtaa aaccagaggc gatcattgcg 360

accaatacct cctcgttgtc gctgaacaaa ctggcggcgg gggtggaaaa caatccgcgc 420

tttatcggcc tgcatttttt caatccggcg ccgctgatga agttgatcga aatcattccc 480

tcttatttta cctcccacac caccagccta cgctgccagc agttggtaat agcgttgggt 540

aaacagtttg tggtctgcaa agccacgccg ggctttattg ttaatcgcat ggcgcgacct 600

ttctatctgg aagggttccg gctgctggag gaaaacgtgg cacaggctcc acagatcgac 660

cgcgccctca aggcaggcgg gcattttcgc atggggccct tagaactgac tgattttatt 720

ggccaggata tcaactatca ggtcagcaag cagatttggc aggatatgca gttcgactcc 780

cgctataccc ccggccattt gcaacgctcg ctggtggatg ccgggctgct ggggaggaag 840

aacgggcgct ctttttttgc ttccctaccg gctacaccgc ccaccccggc cacagagagc 900

gacacaccaa cttcactgca tttttatggt gaacacgctt tattcgatca cctgcaacag 960

cgcgctttgg ccacctggcc tgcgctgcgc gttcagcggt tgccggaacg gccggaactg 1020

gggcgtctta tcctggtgaa taacacgctg gcgatcaaaa tcaccgatgg cagaacggcg 1080

aacctgctcg ccggcttaac cgctctcgac accttcgtga ttgacgctgc gctgaattac 1140

gccgacaccg cctatctggt ggccgcccac aatcagcatg ccacagaggc gaataaagcg 1200

ctgtttctgt cgctgctgca aaccgtcatc gctcaggtag agtttattaa agattcccct 1260

gccctgatcg ttgcccgcgt actgagcagc ctgatcaacg aatcggtgat catggtggag 1320

agcggcgttt gcagccgggc ggatatcgat atcgccgccg tggccggcgt gaactatgcc 1380

gacggcattt ttgcctggct ggcgcagctc gggcagaaaa acgtgaagtc gacgctggat 1440

aacatggcgc aattgctgca ctccgcgcgc tattacccgc attactcatt gctgaacgcg 1500

gcccggcctg agctggctgt agcgccctaa 1530

<210> 166

<211> 1530

<212> DNA

<213> Serratia plymuthica tumat 205

<400> 166

atggcagaga ataattcggc aatccgttca gccgccgtta ttggtgcggg gactatgggc 60

agaggcatcg cctacctctt ggcactgaac ggcatacgaa ccgtacttta taatcgcaat 120

ggtaataatc tcaatcaggc ccgtgactat attgtcagcg acctggacag aaaaatagat 180

aacggaaaaa taaccctgca gaaaaaaggc cagatattag ccaatattat tttctcggcc 240

gtctttgacg ccataaccga cagcgatctg gtgattgaaa ccattgcgga agatgagcaa 300

accaagcatg aaatcctggc agccattgcg gctacggtaa aaccggaggc gatcattgcg 360

accaatacct cctcgttgtc gctgaacaaa ctggcggcag gggtggaaaa caacccgcgc 420

tttatcggcc tgcatttttt caatccggcg ccgctgatga agttgatcga aatcattccc 480

tcttatttta cctcccacgc caccagccta cgctgccagc agttggtaat agcattgggt 540

aaacagtttg tggtctgcaa agccacaccg ggctttattg ttaatcgcat ggcgcggcct 600

ttctatctgg aagggttccg gctgctggag gaaaacgtgg cgctggcgcc acagatcgac 660

cgcgccctca aggccggcgg gcattttcgc atggggcctt tagaactgac ggattttatc 720

ggtcaggata tcaactacca ggtcagcaag cagatttggc aggatatgca gttcgaccct 780

cgctataccc ctggtcattt gcaacgctcg ctggtggatg ccgggctgct ggggaggaaa 840

aacgggcgct ctttttttgc ttcccaaccg gtgacgccac ccaccccgac cacagagagc 900

gacacgccaa cttcactgca tttttatggg gagcatgctt tattcgatca tctgcaacag 960

cgtgctctgg ccacctggcc tgcgctgcgc gttcagcggt tgccggaacg gcctgaactg 1020

gggcgattta tcctggtgaa taacgcgatg gcgatcaaaa tcaccgatgg cagaacggca 1080

aacctgctcg ccggcttaac cgctctcgac accttcgtga ttgacgctgc gctgaattac 1140

gccgacaccg cctatctggt ggccgcccac aatcaacatg ccacagagac gaataaagcg 1200

ctgtttctga cgctgctgca aaccgtcatc gctcaggtgg agtttattaa agattcccct 1260

gctctgatcg ttgcccgcgt actgagcagc ctgatcaatg aatcggtgat catggtggag 1320

agcggcgttt gcagccgggc agatatcgat atcgccgccg tggccggcgt gaactatgcc 1380

gacggcattt ttgcctggtt ggcgcagctc gggcagaaaa acgtgaaatc gacgctggat 1440

aacatggcgc aactgctgca ctccgcgcgc tattacccgc attactcatt gctgaacgcg 1500

gcccggcctg agctggccgt agcgccctaa 1530

<210> 167

<211> 1530

<212> DNA

<213> Serratia plymuthica A30

<400> 167

atggcagaga ataattcggc aatccgttca gccgccgtta ttggtgcggg gaccatgggc 60

agaggcatcg cctacctcct ggcactgaac ggcatacgaa ccgtacttta taatcgcaat 120

ggtaataatc tcaatcaggc ccgtgactat attgtcagcg acctggacag aaaaatagat 180

aacggaaaaa taaccctgca gaaaaaaggc cagatattag ccaatattat tttctcggcc 240

gtctttgacg ccataaccga cagcgatctg gtgattgaaa ccattgcgga agatgagcaa 300

accaagcatg aaatcctggc agccattgcg gctacggtaa aaccggaggc gatcattgcg 360

accaatacct cctcgttgtc gctgaacaaa ctggcggcag gggtggaaaa caacccgcgc 420

tttatcggcc tgcatttttt caatccggcg ccactgatga agttgatcga aatcattccc 480

tcttatttta cctcccacgc caccagccta cgctgccaga agttggtaat agcattgggt 540

aaacagtttg tggtctgcaa agccacgccg ggctttattg ttaatcgcat ggcgcggcct 600

ttctatctgg aagggttccg gctgctggag gagaacgtgg cgctggcgcc acagatcgac 660

cgcgccctca aggccggcgg gcattttcgc atggggcctt tagaactgac ggattttatc 720

ggccaggata tcaactacca ggtcagcaag cagatttggc aggatatgca gttcgaccct 780

cgctataccc ctggtcattt gcaacgctcg ctggtggatg ccgggctgct ggggaggaaa 840

aacgggcgct ctttttttgc ttcccaaccg gcgacgccgc ccaacccgac cactgagggc 900

gacacgccaa cttcactgca tttttatggt gaacacgctt tattcgatca cctgcaacag 960

cgcgctttgg ccacctggcc tgcgttgcgc gttcagcggt tgccggaacg gccggaactg 1020

gggcgtttta tcctgatgaa taacaggctg gcgatcaaaa tcactgatgg cagaacggcg 1080

aacctgctcg ccggcttaac cgctctcgac accttcgtga ttgacgccgc gctgaactac 1140

gccgacaccg cctatctggt ggccgcccac aatcaacatg ccacagagac gaataaagcg 1200

ctgtttctga cgctgctgca aaccctcatc gctcaggtgg agtttattaa agattcccct 1260

gccctgatcg ttgcccgcgt actgagcagc ctgatcaatg aatcggtgat catggtggag 1320

agcggcgttt gcagccgggc agatatcgat atcgccgccg tggccggcgt gaactatgcc 1380

gacggtattt ttgcctggtt ggcgcagctc gggcagaaaa acgtgaaatc gacgctggat 1440

aacatggcgc aactgctgca ctccgcgcgc tattacccgc attactcatt gctgaacgcg 1500

gcccggcctg agctggccgt agcgccctaa 1530

<210> 168

<211> 1530

<212> DNA

<213> Serratia plymuthica 4Rx13

<400> 168

atggcagaga ataattcggc aatccgttca gccgccgtta ttggtgcggg gaccatgggc 60

agaggcatcg cctacctcct ggcgctgaac ggcatacgaa ccgtacttta taatcgcaat 120

ggtaatagtc tcaatcaggc ccgtgactat attgtcagcg acctggacag aaaaatagat 180

aacggaaaaa taaccctgca gaaaaaaggc cagatattag ccaatattat tttctcggcc 240

gtctttgacg ccataaccga cagcgatctg gtgattgaaa ccattgcgga agatgagcaa 300

accaagcatg aaatcctggc agccattgcg gctacggtaa aaccggaggc gatcattgcg 360

accaatacct cctcgttgtc gctgaacaaa ctggcggcag gggtggaaaa caacccgcgc 420

tttatcggcc tgcatttttt caatccggcg ccgctgatga agttgatcga aatcattccc 480

tcttatttta cctcccacgc caccagccta cgctgccaga agttggtaat agcattgggt 540

aaacagtttg tggtctgcaa agccacgccg ggctttattg ttaatcgcat ggcgcggcct 600

ttctatctgg aagggttccg gctgctggag gagaacgtgg cgctggcgcc acagatcgac 660

cgcgccctca aggccggcgg gcattttcgc atggggcctt tagaactgac ggattttatc 720

ggtcaggata tcaactacca ggtcagcaag cagatttggc aggatatgca gttcgaccct 780

cgctataccc ccggccactt gcaacgctcg ctggtggatg ccgggctgct ggggaggaaa 840

aacgggcgtt ctttttttgc ttcccaaccg gcgacgccac ccaccccgac cacagagagc 900

gacacgccaa cttcactgca tttttatggt gagcacgctt tattcgatca tctgcaacag 960

cgcgctctgg ccacctggcc tgcgctgagc gttcagcggt tgccggaacg gcctgaactg 1020

gggcgattta tcctggtgaa taacgcgctg gcgatcaaaa tcaccgatgg cagaacggca 1080

aacctgctcg ccggcttaac cgctctcgac accttcgtga ttgacgctgc gctgaattac 1140

gccgacaccg cctatctggt ggccgcccac aatcaacatg ccacagagac gaataaagcg 1200

ctgtttctga cgctgctgca aaccgtcatc gctcaggtgg agtttattaa agattcccct 1260

gccctgatcg ttgcccgcgt actgagcagc ctgatcaatg aatcggtgat catggtggag 1320

agcggcgttt gcagccgggc agatatcgat atcgccgccg tggccggcgt gaactatgcc 1380

gacggcattt ttgcctggtt ggcgcagctc gggcagaaaa acgtgaaatc gacgctggat 1440

aacatggcgc aactgctgca ttccgcgcgc tattacccgc attactcatt gctgaacgcg 1500

gcccggcctg agctggccgt agcgccctaa 1530

<210> 169

<211> 1530

<212> DNA

<213> Serratia plymuthica V4

<400> 169

atggcagaga ataattcggc aatccgttca gccgccgtta ttggtgcggg gaccatgggc 60

agaggcatcg cctacctcct ggcactgaac ggcatacgaa ccgtacttta taatcgcaat 120

ggtaataatc tcaatcaggc ccgtgactat attgtcagcg acctggacag aaaaatagat 180

aacggaaaaa taacccttca gaaaaaaggc cagatattag ccaatattat tttctcggcc 240

gtctttgacg ccataaccga cagcgatctg gtgattgaaa ccattgcgga agatgagcaa 300

accaagcatg aaatcctggc agccattgcg gctacggtaa aaccggaggc gatcattgcg 360

accaatacct cctcgttgtc gctgaacaaa ctggcggcag gggtggaaaa caacccgcgc 420

tttatcggcc tgcatttttt caatccggcg ccgctgatga agttgatcga aatcattccc 480

tcttatttta cctcccacgc caccagccta cgctgccaga agttggtaat agcattgggt 540

aaacagtttg tggtctgcaa agccacgccg ggctttattg ttaatcgcat ggcgcggcct 600

ttctatctgg aagggttccg gctgctggag gaaaacgtgg cgctggcgcc acagatcgac 660

cgcgccctca aggccggcgg gcattttcgc atgggccctt tagaactgac ggattttatc 720

ggccaggata tcaactacca ggtcagcaag cagatttggc aggatatgca gttcgaccct 780

cgctataccc ctggtcattt gcaacgctcg ctggtggatg ccgggctgct ggggaggaaa 840

aacgggcgct ctttttttgc ttcccaaccg gcgacgccac ccaccccgac cacagagagc 900

gacacgccaa cttcactgca tttttatggt gaacacgctt tattcgatca cctgcaacag 960

cgcgctttgg ccacctggcc tgcgttgcgc gttcagcggt tgccggaacg gccggaactg 1020

gggcgtttta tcctggtgaa taacaggctg gcgatcaaaa tcaccgatgg cagaacggca 1080

aacctgctcg ccggcttaac cgctctcgac accttcgtga ttgacgctgc gctgaattac 1140

gccgacaccg cctatctggt ggccgcccac aatcaacatg ccacagagac gaataaagcg 1200

ctgtttctga cgctgctgca aaccgtcatc gctcaggtgg agtttattaa agattcccct 1260

gccctgatcg ttgcccgcgt actgagcagc ctgatcaatg aatcggtgat catggtggag 1320

agcggcgttt gcagccgggc agatatcgat atcgccgccg tggccggcgt gaactatgcc 1380

gacggtattt ttgcctggtt ggcgcagctc gggcagaaaa acgtgaaatc gacgctggat 1440

aacatggcgc aactgctgca ctccgcgcgc tattacccgc attactcatt gctgaacgcg 1500

gcccggcctg agctggccgt agcgccctaa 1530

<210> 170

<211> 1530

<212> DNA

<213> Serratia plymuthica 3Rp8

<400> 170

atggcagaga ataattcggc aatccgttca gccgccgtta ttggtgcggg gaccatgggc 60

agaggcatcg cctacctcct ggcactgaac ggcatacgaa ccgtacttta taatcgcaat 120

ggtaataatc tcaatcaggc ccgtgactat attgtcagcg acctggacag aaaaatagat 180

aacggaaaaa taaccctgca gaaaaaaggc cagatattag ccaatattat tttctcggcc 240

gtctttgacg ccataaccga cagcgatctg gtgattgaaa ccattgcgga agatgagcaa 300

accaagcatg aaatcctggc agccattgcg gctacggtaa aaccggaggc gatcattgcg 360

accaatacct cctcgttgtc gctgaacaaa ctggcggcag gggtggaaaa caacccgcgc 420

tttatcggcc tgcatttttt caatccggcg ccgctgatga agttgatcga aatcattccc 480

tcttatttta cctcccacgc caccagccta cgctgccaga agttggtaat agcattgggt 540

aaacagtttg tggtctgcaa agccacgccg ggctttattg ttaatcgcat ggcgcggcct 600

ttctatctgg aagggttccg gctgctggag gagaacgtgg cgctggcgcc acagatcgac 660

cgcgccctca aggccggcgg gcattttcgc atggggcctt tagaactgac ggattttatc 720

ggccaggata tcaactacca ggtcagcaag cagatttggc aggatatgca gttcgaccct 780

cgctataccc ctggtcattt gcaacgctcg ctggtggatg ccgggctgct ggggaggaaa 840

aacgggcgct ctttttttgc ttcccaaccg gcgacgccac ccaccccgac cacagagagc 900

gacacgccaa cgtcactgca tttttatggt gaacacgctt tattcgatca cctgcaacag 960

cgcgctttgg ccacctggcc tgcgttgcgc gttcagcggt tgccggaacg gccggaactg 1020

gggcgtttta tcctggtgaa taacaggctg gcgatcaaaa tcaccgatgg cagaacggcg 1080

aacctgctcg ccggcttaac cgctctcgac accttcgtga ttgacgccgc gctgaactac 1140

gccgacaccg cctatctggt ggccgcccac aatcaacatg ccacagagac gaataaagcg 1200

ctgtttctga cgctgctgca aaccctcatc gctcaggtgg agtttattaa agattcccct 1260

gctctgatcg ttgcccgcgt actgagcagc ctgatcaatg aatcggtgat catggtggag 1320

agcggcgttt gcagccgggc agatatcgat atcgccgccg tggccggcgt gaactatgcc 1380

gacggcattt ttgcctggtt ggcgcagctc gggcagaaaa acgtgaaatc gacgctggat 1440

aacatggcgc aactgctgca ctccgcgcgc tattacccgc attactcatt gctgaacgcg 1500

gcccggcctg agctggccgt agcgccctaa 1530

<210> 171

<211> 1530

<212> DNA

<213> Serratia proteamaculans MFPA44A14

<400> 171

atggcagaga ataattcggc aatccattcg gtcgctgtta ttggtgccgg aaccatgggc 60

agaggtattg cctatcttct ggcgcagaac ggcatacgaa ccctgcttta taatcgtagc 120

ggtaataatc tggatcaggc ccgcgactat attatccgtg acctggataa gaaaatagac 180

aacgggaaaa taagcccaca gaaaaaaggc gaagtattgg ccaacctggt tttctctccc 240

attttcgatg ctatcgccga cagcgacctg gtgattgaaa ccatcgcgga gcatgagacc 300

accaagcatg aaatcctcgc ggcgattgcg gccacggtga aacaagaggc cattatcgcc 360

accaacacct catcgctgtc gttgaataag ctggcggcag gcgtcgaaaa caacgcccgt 420

tttatcggcc tgcacttctt caatccggcc ccgctgatga aactgatcga gattattccg 480

tcctatttta ccagccgggc caccagcctg cgctgccagc agttggtgac ggcgctaggc 540

aaacagtttg tggtctgcaa agccacgccg ggttttatcg tcaaccggat ggcacggcct 600

ttttatctgg aaggattccg gctgttggaa gaaaacgtgg cattagcacc gcagatcgac 660

cgcgccctca aggccggtgg ccactttcgc atgggccctt tggagctgac cgactttatc 720

ggccaggata ttaactatca ggtcagcagc cagatttggc aggacatgca atacgacccc 780

cgctataccc ccggtcattt gcaacgttcg ctggtggatg ccgggttgct ggggaagaaa 840

aacggccgat ccttttttgc tgccccttct gccgaatcca acctcctcga cgcaggcaac 900

ggtacgctga cttccctgca tttttatggc gaacataccc tgtttgacct gctgcaacag 960

cgcgccttgg ctacttggcc aacgctgcag attattcacc agccggaacg gccaacgctg 1020

ggacgcttta tccgggtgaa tgacgcattg gccgtcaaaa tcaccgacgg ccgcaccgcc 1080

aatctgctcg ctgaattgac cgatctcgac acctttgtga tcgacgccgc actgaattac 1140

agcgatacca cctatctggt ggccgcccac aatcaggacg ccgccgaggc caataaagcg 1200

ctgtttctgt cgctgctgca aacgttgatc ccgcaggtgg agtttattaa agactctcca 1260

ggcctgatcg tcgcccgggt tctgagcagt ctgatcaatg agtcggtgat catggtggag 1320

agcggggttt gcagccgggc agatatcgat attgccgccg tggcgggcgt taactatgcc 1380

gatggcatct ttgcctggct gacgcagctc gggcaaaaaa acgtgaaatc aacgctggat 1440

aatatggcgc aactgctgca ttccgcccgc tattacccgc attactcatt gctgaatatc 1500

ccccggcccg agctggccgt cgcgccgtaa 1530

<210> 172

<211> 1530

<212> DNA

<213> Serratia plymuthica A153

<400> 172

atggcagaga ataattcggc aatccgttca gccgccgtta ttggtgcggg gaccatgggc 60

agaggcatcg cctatctcct ggcgctgaac ggcatacgaa ccgtacttta taatcgcaat 120

ggtaataatc tcaatcaggc ccgtgactat attgtcagcg acctggacag aaaaatagat 180

aacggaaaaa taaccctgca gaaaaaaggc cagatattag ccaatattat tttctcggac 240

gtctttgacg ccataaccga cagcgatctg gtgattgaaa ccattgcgga agatgagcaa 300

accaagcatg aaatcctggc agccattgcg gcgacggtaa aaccggaggc gatcattgcg 360

accaatactt cctcgctgtc gctgaacaaa ctggcggcgg gggtggaaaa caacccgcgc 420

tttatcggcc tgcatttttt caatccggcg ccgctgatga agttgatcga aattattccc 480

tcttatttca cctctcgcgc caccagtcta cgctgccagc agttggtaac agcgttgggt 540

aaactgtttg tggtctgcaa agccacgccg ggctttattg ttaatcgcat ggcgcggcct 600

ttctatctgg aagggttccg gctgctggag gaaaacgtgg cgctggctcc acagatcgac 660

cgcgccctca aggccggcgg gcattttcgc atggggcctt tagaactgac tgattttatc 720

ggccaggata tcaactatca ggtcagcaaa cagatttggc aggatatgca gttcgatccc 780

cgctataccc ccggccattt gcaacgctcg ctggtggatg ccgggctgct ggggaggaaa 840

aacgggcgct ctttttttgc ttcccaacct gctacaccgc ccaacccgac cactgagggc 900

cacacgccaa cttcactgct tttttatggt gaacacgctt tattcgatca cctgcaacag 960

cgcgctttgg ccacctggcc tgcgctgcgc gttcagcggt tgccggaacg gccggaactg 1020

ggacgtttta tcctggtgaa taacaggctg gcgatcaaaa tcaccgatgg cagaacggcg 1080

aacctgctcg ccggcttaac cgctctcgac accttcgtga ttgacgccgc gctgaactac 1140

gccgacaccg cctatctggt ggccgcccac aatcaacatg ccacagagcc gaataaagcg 1200

ctgtttctga cgctgctgca aaccctcatc gctcaggtgg agtttattaa agattcccct 1260

gccctgatcg ttgcccgcgt actgagcagc ctgatcaatg aatcggtgat catggtggag 1320

agcggcgttt gcagccgggc agatatcgat atcgccgccg tggccggcgt gaactatgcc 1380

gacggcattt ttgcctggtt ggcgcagctc gggcagaaaa acgtgaaatc gacgctggat 1440

aacatggcgc aattgctgca ctccacgcgc tattacccgc attactcatt gctgaacgcg 1500

gcccggcctg agctggctgt agcgccctaa 1530

<210> 173

<211> 24

<212> PRT

<213> Artificial Sequence

<220>

<223> consensus sequence 1

<220>

<221> misc_feature

<222> (13)..(13)

<223> Xaa can be any naturally occurring amino acid

<220>

<221> misc_feature

<222> (15)..(17)

<223> Xaa can be any naturally occurring amino acid

<220>

<221> misc_feature

<222> (19)..(19)

<223> Xaa can be any naturally occurring amino acid

<220>

<221> misc_feature

<222> (21)..(21)

<223> Xaa can be any naturally occurring amino acid

<400> 173

Gly Ala Gly Thr Met Gly Arg Gly Ile Ala Tyr Leu Xaa Ala Xaa Xaa

1 5 10 15

Xaa Ile Xaa Thr Xaa Leu Tyr Asn

20

<210> 174

<211> 774

<212> DNA

<213> Pseudomonas putida KT2440

<400> 174

atgccgcgat atatcgatgt gcaggcgccc gaacatggcg ttcagctcat taccctgcaa 60

cggcccgagg ccttgaatgc cctgtgcacc gagctactgg cagaactggc cgctgcgctg 120

caggctgccg ggaacgacga gcatgtccgt gccacagtga ttaccggcag cgccaaggca 180

ttcgccgcag gcgccgacat ccgcgagatg gccgatcgcg acctggtcgg catcctcaat 240

gacccgcgcg tagcgcattg gcaaagcatc gccgcattcg ccaaaccgct gattgctgca 300

gtcaacggct atgccctggg tggcggttgc gaactggcaa tgtgcgccga catcgtcatc 360

gccagtaccg acgcccgttt cggccagccg gaaatcaacc ttggcatcat ccccggtgct 420

ggcggcaccc agcgcctgtt acgtgccgtc ggtaagccgt tggccatgca gatggtgctg 480

acgggggaag ccatcactgc cctccgcgcc cagcaggccg gcctggtcag cgaaatcacc 540

cagcccgaac tcaccgtaga acgcgccatg caggttgccc gcagcatcgc cgccaaagcg 600

ccgctggctg tgcgcctggc caaggaggcg ttactgaagg ccggtgatac cgacctggcc 660

agcggcctgc gcttcgagcg ccatgccttc accctgctgg cgggcaccgc cgaccgcgat 720

gaaggcatcc gcgccttcca ggaaaagcgc caggcccgct tccaagggcg ctga 774

<210> 175

<211> 696

<212> DNA

<213> Pseudomonas putida KT2440

<400> 175

ttgatcaata aaacgtacga gtccatcgcc agcgcggtgg aagggattac cgacggttcg 60

accatcatgg tcggtggctt cggcacggct ggcatgccgt ccgagctgat cgatggcctc 120

attgccaccg gtgcccgcga cctgaccatc atcagcaaca acgccggcaa cggcgagatc 180

ggcctggccg ccctgctcat ggcaggcagc gtgcgcaagg tggtctgctc gttcccgcgc 240

cagtccgact cctacgtgtt cgacgaactg taccgcgccg gcaagatcga gctggaagtg 300

gtcccgcagg gcaacctggc cgagcgtatc cgcgccgcag gctccggcat tggtgcgttc 360

ttctcgccaa ccggctacgg caccctgctg gccgagggca aggaaacccg tgagatcgat 420

ggccgcatgt acgtgctgga aatgccgctg cacgccgact tcgcactgat caaggcgcac 480

aagggtgacc gttggggcaa cctgacctac cgcaaggccg cccgcaactt cggcccgatc 540

atggccatgg ctgccaagac cgccatcgcc caggtcgacc aggtcgtcga actcggtgaa 600

ctggacccgg aacacatcat caccccgggt atcttcgtcc agcgcgtggt cgccgtcacc 660

ggtgctgccg cttcttcgat tgccaaagct gtctga 696

<210> 176

<211> 642

<212> DNA

<213> Pseudomonas putida KT2440

<400> 176

atgaccatca ccaaaaagct ctcccgcacc gagatggccc aacgcgtggc cgcagacatc 60

caggaaggcg cgtacgtaaa cctgggcatc ggcgcaccga ccctggtggc caactacctg 120

ggcgacaagg aagtgttcct gcacagcgag aacggcctgc tgggcatggg cccaagccct 180

gcgccgggcg aggaagacga tgacctgatc aacgccggca agcagcacgt caccctgctg 240

accggtggtg ccttcttcca ccatgccgat tcgttctcga tgatgcgtgg cggccacctg 300

gacatcgctg tactgggcgc cttccaggtg tcggtcaagg gcgacctggc caactggcac 360

acgggtgccg aaggctcgat cccggccgta ggcggtgcaa tggacctggc caccggcgcc 420

cgccaggtgt tcgtgatgat ggaccacctg accaagaccg gcgaaagcaa gctggtgccc 480

gagtgcacct acccgctgac cggtatcgct tgcgtcagcc gcatctacac cgacctggcc 540

gtactggaag tgacacctga agggctgaaa gtggtcgaaa tctgcgcgga catcgacttt 600

gacgagctgc agaaactcag tggcgtgccg ctgatcaagt ga 642

<210> 177

<211> 774

<212> DNA

<213> Pseudomonas putida KT2440

<400> 177

atgccgcgat atatcgatgt gcaggcgccc gaacatggcg ttcagctcat taccctgcaa 60

cggcccgagg ccttgaatgc cctgtgcacc gagctactgg cagaactggc cgctgcgctg 120

caggctgccg ggaacgacga gcatgtccgt gccacagtga ttaccggcag cgccaaggca 180

ttcgccgcag gcgccgacat ccgcgagatg gccgatcgcg acctggtcgg catcctcaat 240

gacccgcgcg tagcgcattg gcaaagcatc gccgcattcg ccaaaccgct gattgctgca 300

gtcaacggct atgccctggg tggcggttgc gaactggcaa tgtgcgccga catcgtcatc 360

gccagtaccg acgcccgttt cggccagccg gaaatcaacc ttggcatcat ccccggtgct 420

ggcggcaccc agcgcctgtt acgtgccgtc ggtaagccgt tggccatgca gatggtgctg 480

acgggggaag ccatcactgc cctccgcgcc cagcaggccg gcctggtcag cgaaatcacc 540

cagcccgaac tcaccgtaga acgcgccatg caggttgccc gcagcatcgc cgccaaagcg 600

ccgctggctg tgcgcctggc caaggaggcg ttactgaagg ccggtgatac cgacctggcc 660

agcggcctgc gcttcgagcg ccatgccttc accctgctgg cgggcaccgc cgaccgcgat 720

gaaggcatcc gcgccttcca ggaaaagcgc caggcccgct tccaagggcg ctga 774

<210> 178

<211> 470

<212> PRT

<213> Escherichia coli str. K-12 substr. MG1655

<400> 178

Met Lys Lys Thr Lys Ile Val Cys Thr Ile Gly Pro Lys Thr Glu Ser

1 5 10 15

Glu Glu Met Leu Ala Lys Met Leu Asp Ala Gly Met Asn Val Met Arg

20 25 30

Leu Asn Phe Ser His Gly Asp Tyr Ala Glu His Gly Gln Arg Ile Gln

35 40 45

Asn Leu Arg Asn Val Met Ser Lys Thr Gly Lys Thr Ala Ala Ile Leu

50 55 60

Leu Asp Thr Lys Gly Pro Glu Ile Arg Thr Met Lys Leu Glu Gly Gly

65 70 75 80

Asn Asp Val Ser Leu Lys Ala Gly Gln Thr Phe Thr Phe Thr Thr Asp

85 90 95

Lys Ser Val Ile Gly Asn Ser Glu Met Val Ala Val Thr Tyr Glu Gly

100 105 110

Phe Thr Thr Asp Leu Ser Val Gly Asn Thr Val Leu Val Asp Asp Gly

115 120 125

Leu Ile Gly Met Glu Val Thr Ala Ile Glu Gly Asn Lys Val Ile Cys

130 135 140

Lys Val Leu Asn Asn Gly Asp Leu Gly Glu Asn Lys Gly Val Asn Leu

145 150 155 160

Pro Gly Val Ser Ile Ala Leu Pro Ala Leu Ala Glu Lys Asp Lys Gln

165 170 175

Asp Leu Ile Phe Gly Cys Glu Gln Gly Val Asp Phe Val Ala Ala Ser

180 185 190

Phe Ile Arg Lys Arg Ser Asp Val Ile Glu Ile Arg Glu His Leu Lys

195 200 205

Ala His Gly Gly Glu Asn Ile His Ile Ile Ser Lys Ile Glu Asn Gln

210 215 220

Glu Gly Leu Asn Asn Phe Asp Glu Ile Leu Glu Ala Ser Asp Gly Ile

225 230 235 240

Met Val Ala Arg Gly Asp Leu Gly Val Glu Ile Pro Val Glu Glu Val

245 250 255

Ile Phe Ala Gln Lys Met Met Ile Glu Lys Cys Ile Arg Ala Arg Lys

260 265 270

Val Val Ile Thr Ala Thr Gln Met Leu Asp Ser Met Ile Lys Asn Pro

275 280 285

Arg Pro Thr Arg Ala Glu Ala Gly Asp Val Ala Asn Ala Ile Leu Asp

290 295 300

Gly Thr Asp Ala Val Met Leu Ser Gly Glu Ser Ala Lys Gly Lys Tyr

305 310 315 320

Pro Leu Glu Ala Val Ser Ile Met Ala Thr Ile Cys Glu Arg Thr Asp

325 330 335

Arg Val Met Asn Ser Arg Leu Glu Phe Asn Asn Asp Asn Arg Lys Leu

340 345 350

Arg Ile Thr Glu Ala Val Cys Arg Gly Ala Val Glu Thr Ala Glu Lys

355 360 365

Leu Asp Ala Pro Leu Ile Val Val Ala Thr Gln Gly Gly Lys Ser Ala

370 375 380

Arg Ala Val Arg Lys Tyr Phe Pro Asp Ala Thr Ile Leu Ala Leu Thr

385 390 395 400

Thr Asn Glu Lys Thr Ala His Gln Leu Val Leu Ser Lys Gly Val Val

405 410 415

Pro Gln Leu Val Lys Glu Ile Thr Ser Thr Asp Asp Phe Tyr Arg Leu

420 425 430

Gly Lys Glu Leu Ala Leu Gln Ser Gly Leu Ala His Lys Gly Asp Val

435 440 445

Val Val Met Val Ser Gly Ala Leu Val Pro Ser Gly Thr Thr Asn Thr

450 455 460

Ala Ser Val His Val Leu

465 470

<210> 179

<211> 480

<212> PRT

<213> Escherichia coli str. K-12 substr. MG1655

<400> 179

Met Ser Arg Arg Leu Arg Arg Thr Lys Ile Val Thr Thr Leu Gly Pro

1 5 10 15

Ala Thr Asp Arg Asp Asn Asn Leu Glu Lys Val Ile Ala Ala Gly Ala

20 25 30

Asn Val Val Arg Met Asn Phe Ser His Gly Ser Pro Glu Asp His Lys

35 40 45

Met Arg Ala Asp Lys Val Arg Glu Ile Ala Ala Lys Leu Gly Arg His

50 55 60

Val Ala Ile Leu Gly Asp Leu Gln Gly Pro Lys Ile Arg Val Ser Thr

65 70 75 80

Phe Lys Glu Gly Lys Val Phe Leu Asn Ile Gly Asp Lys Phe Leu Leu

85 90 95

Asp Ala Asn Leu Gly Lys Gly Glu Gly Asp Lys Glu Lys Val Gly Ile

100 105 110

Asp Tyr Lys Gly Leu Pro Ala Asp Val Val Pro Gly Asp Ile Leu Leu

115 120 125

Leu Asp Asp Gly Arg Val Gln Leu Lys Val Leu Glu Val Gln Gly Met

130 135 140

Lys Val Phe Thr Glu Val Thr Val Gly Gly Pro Leu Ser Asn Asn Lys

145 150 155 160

Gly Ile Asn Lys Leu Gly Gly Gly Leu Ser Ala Glu Ala Leu Thr Glu

165 170 175

Lys Asp Lys Ala Asp Ile Lys Thr Ala Ala Leu Ile Gly Val Asp Tyr

180 185 190

Leu Ala Val Ser Phe Pro Arg Cys Gly Glu Asp Leu Asn Tyr Ala Arg

195 200 205

Arg Leu Ala Arg Asp Ala Gly Cys Asp Ala Lys Ile Val Ala Lys Val

210 215 220

Glu Arg Ala Glu Ala Val Cys Ser Gln Asp Ala Met Asp Asp Ile Ile

225 230 235 240

Leu Ala Ser Asp Val Val Met Val Ala Arg Gly Asp Leu Gly Val Glu

245 250 255

Ile Gly Asp Pro Glu Leu Val Gly Ile Gln Lys Ala Leu Ile Arg Arg

260 265 270

Ala Arg Gln Leu Asn Arg Ala Val Ile Thr Ala Thr Gln Met Met Glu

275 280 285

Ser Met Ile Thr Asn Pro Met Pro Thr Arg Ala Glu Val Met Asp Val

290 295 300

Ala Asn Ala Val Leu Asp Gly Thr Asp Ala Val Met Leu Ser Ala Glu

305 310 315 320

Thr Ala Ala Gly Gln Tyr Pro Ser Glu Thr Val Ala Ala Met Ala Arg

325 330 335

Val Cys Leu Gly Ala Glu Lys Ile Pro Ser Ile Asn Val Ser Lys His

340 345 350

Arg Leu Asp Val Gln Phe Asp Asn Val Glu Glu Ala Ile Ala Met Ser

355 360 365

Ala Met Tyr Ala Ala Asn His Leu Lys Gly Val Thr Ala Ile Ile Thr

370 375 380

Met Thr Glu Ser Gly Arg Thr Ala Leu Met Thr Ser Arg Ile Ser Ser

385 390 395 400

Gly Leu Pro Ile Phe Ala Met Ser Arg His Glu Arg Thr Leu Asn Leu

405 410 415

Thr Ala Leu Tyr Arg Gly Val Thr Pro Val His Phe Asp Ser Ala Asn

420 425 430

Asp Gly Val Ala Ala Ala Ser Glu Ala Val Asn Leu Leu Arg Asp Lys

435 440 445

Gly Tyr Leu Met Ser Gly Asp Leu Val Ile Val Thr Gln Gly Asp Val

450 455 460

Met Ser Thr Val Gly Ser Thr Asn Thr Thr Arg Ile Leu Thr Val Glu

465 470 475 480

<210> 180

<211> 470

<212> PRT

<213> Serratia grimesii NBRC13537

<400> 180

Met Lys Lys Thr Lys Ile Val Cys Thr Ile Gly Pro Lys Thr Glu Ser

1 5 10 15

Glu Glu Met Leu Thr Asn Leu Leu Asn Ala Gly Met Asn Val Met Arg

20 25 30

Leu Asn Phe Ser His Gly Asp Tyr Glu Glu His Gly Asn Arg Ile Lys

35 40 45

Asn Met Arg Ala Val Met Ala Lys Thr Gly Gln Asn Ala Gly Ile Leu

50 55 60

Leu Asp Thr Lys Gly Pro Glu Ile Arg Thr Met Lys Leu Glu Gly Gly

65 70 75 80

Lys Asp Ala Ala Leu Val Ala Gly Gln Thr Phe Thr Phe Thr Thr Asp

85 90 95

Gln Ser Val Ile Gly Asn Asn Glu Arg Val Ala Val Thr Tyr Ala Gly

100 105 110

Phe Ser Ala Asp Leu Lys Ile Gly Asn Thr Val Leu Val Asp Asp Gly

115 120 125

Leu Ile Gly Met Glu Val Thr Asn Val Thr Glu Asn Glu Val Val Cys

130 135 140

Lys Val Leu Asn Ser Gly Asp Leu Gly Glu Asn Lys Gly Val Asn Leu

145 150 155 160

Pro Gly Val Ser Ile Gln Leu Pro Ala Leu Ala Glu Lys Asp Lys Arg

165 170 175

Asp Leu Ile Phe Gly Cys Glu Gln Gly Val Asp Phe Val Ala Ala Ser

180 185 190

Phe Ile Arg Lys Arg Ser Asp Val Leu Glu Ile Arg Glu His Leu Lys

195 200 205

Ala His Gly Gly Glu Gln Ile Gln Ile Ile Ser Lys Ile Glu Asn Gln

210 215 220

Glu Gly Leu Asn Asn Phe Asp Glu Ile Leu Glu Ala Ser Asp Gly Ile

225 230 235 240

Met Val Ala Arg Gly Asp Leu Gly Val Glu Ile Pro Val Glu Glu Val

245 250 255

Ile Phe Ala Gln Lys Met Met Ile Glu Lys Cys Asn Arg Ala Arg Lys

260 265 270

Val Val Ile Thr Ala Thr Gln Met Leu Asp Ser Met Ile Lys Asn Pro

275 280 285

Arg Pro Thr Arg Ala Glu Ala Gly Asp Val Ala Asn Ala Ile Leu Asp

290 295 300

Gly Thr Asp Ala Val Met Leu Ser Gly Glu Ser Ala Lys Gly Lys Tyr

305 310 315 320

Pro Leu Glu Ala Val Thr Ile Met Ala Thr Ile Cys Glu Arg Thr Asp

325 330 335

Arg Val Met Pro Ser Arg Ile Asp Ser Leu Asn Asp Asn Arg Lys Leu

340 345 350

Arg Ile Thr Glu Ala Val Cys Arg Gly Ala Val Glu Thr Ala Glu Lys

355 360 365

Leu Asp Ala Pro Leu Ile Val Val Ala Thr Ser Gly Gly Lys Ser Ala

370 375 380

Lys Ser Val Arg Lys Tyr Phe Pro Asn Ala Val Ile Leu Ala Leu Thr

385 390 395 400

Thr Asn Glu Val Thr Ala His Gln Leu Ile Leu Ser Lys Gly Val Ile

405 410 415

Pro Gln Met Val Lys Glu Ile Ala Ser Thr Asp Asp Phe Tyr Arg Ile

420 425 430

Gly Lys Glu Ala Ala Leu Ala Ser Gly Leu Ala Gln Lys Gly Asp Val

435 440 445

Val Val Met Val Ser Gly Ala Leu Val Pro Ser Gly Thr Thr Asn Thr

450 455 460

Ala Ser Val His Val Leu

465 470

<210> 181

<211> 480

<212> PRT

<213> Serratia grimesii NBRC13537

<400> 181

Met Ser Arg Arg Leu Arg Arg Thr Lys Ile Val Thr Thr Leu Gly Pro

1 5 10 15

Ala Thr Asp Arg Asp Asn Asn Leu Glu Lys Ile Ile Ala Ala Gly Ala

20 25 30

Asn Val Val Arg Leu Asn Phe Ser His Gly Ser Ala Glu Asp His Gln

35 40 45

Ala Arg Ala Asp Lys Val Arg Glu Ile Ala Ala Lys Leu Gly Arg His

50 55 60

Val Ala Ile Leu Gly Asp Leu Gln Gly Pro Lys Ile Arg Val Ser Thr

65 70 75 80

Phe Lys Glu Gly Lys Ile Phe Leu Asn Ile Gly Asp Lys Phe Leu Leu

85 90 95

Asp Ala Asn Met Ser Lys Gly Glu Gly Asp Lys Glu Lys Val Gly Ile

100 105 110

Asp Tyr Lys Gly Leu Pro Ala Asp Val Val Pro Gly Asp Val Leu Leu

115 120 125

Leu Asp Asp Gly Arg Val Gln Leu Lys Val Leu Glu Val Gln Gly Met

130 135 140

Lys Val Phe Thr Glu Val Thr Val Gly Gly Pro Leu Ser Asn Asn Lys

145 150 155 160

Gly Ile Asn Lys Leu Gly Gly Gly Leu Ser Ala Glu Ala Leu Thr Glu

165 170 175

Lys Asp Lys Ala Asp Ile Val Thr Ala Ala Lys Ile Gly Val Asp Tyr

180 185 190

Leu Ala Val Ser Phe Pro Arg Thr Gly Glu Asp Leu Asn Tyr Ala Arg

195 200 205

Arg Leu Ala Arg Asp Ala Gly Cys Asn Ala Lys Ile Val Ser Lys Val

210 215 220

Glu Arg Ala Glu Ala Val Cys Ser Asp Glu Ala Met Asp Asp Ile Ile

225 230 235 240

Leu Ala Ser Asp Val Val Met Val Ala Arg Gly Asp Leu Gly Val Glu

245 250 255

Ile Gly Asp Pro Glu Leu Val Gly Ile Gln Lys Lys Leu Ile Arg Arg

260 265 270

Ala Arg Thr Leu Asn Arg Ala Val Ile Thr Ala Thr Gln Met Met Glu

275 280 285

Ser Met Ile Thr Asn Pro Met Pro Thr Arg Ala Glu Val Met Asp Val

290 295 300

Ala Asn Ala Val Leu Asp Gly Thr Asp Ala Val Met Leu Ser Ala Glu

305 310 315 320

Thr Ala Ala Gly Gln Tyr Pro Ala Glu Thr Val Ala Ala Met Ala Arg

325 330 335

Val Cys Leu Gly Ala Glu Lys Ile Pro Ser Ile Asn Val Ser Lys His

340 345 350

Arg Leu Asp Val Gln Phe Asp Asn Ile Glu Glu Ala Ile Ala Met Ser

355 360 365

Ser Met Tyr Ala Ala Asn His Leu Lys Gly Val Thr Ala Leu Ile Ala

370 375 380

Met Thr Glu Ser Gly Arg Thr Ala Leu Met Met Ser Arg Ile Ser Ser

385 390 395 400

Gly Leu Pro Ile Phe Ala Met Ser Arg His Glu His Thr Leu Asn Leu

405 410 415

Thr Ala Leu Tyr Arg Gly Val Thr Pro Val Tyr Phe Asp Ser His Glu

420 425 430

Asp Gly Val Ile Ala Ala Asn Asp Ala Val Asn Arg Leu Arg Asp Lys

435 440 445

Gly Phe Leu Val Ser Gly Asp Leu Val Ile Val Thr Gln Gly Asp Val

450 455 460

Met Glu Thr Val Gly Thr Thr Asn Thr Ser Arg Ile Leu Arg Val Glu

465 470 475 480

<210> 182

<211> 540

<212> PRT

<213> Escherichia coli str. K-12 substr. MG1655

<400> 182

Met Arg Val Asn Asn Gly Leu Thr Pro Gln Glu Leu Glu Ala Tyr Gly

1 5 10 15

Ile Ser Asp Val His Asp Ile Val Tyr Asn Pro Ser Tyr Asp Leu Leu

20 25 30

Tyr Gln Glu Glu Leu Asp Pro Ser Leu Thr Gly Tyr Glu Arg Gly Val

35 40 45

Leu Thr Asn Leu Gly Ala Val Ala Val Asp Thr Gly Ile Phe Thr Gly

50 55 60

Arg Ser Pro Lys Asp Lys Tyr Ile Val Arg Asp Asp Thr Thr Arg Asp

65 70 75 80

Thr Phe Trp Trp Ala Asp Lys Gly Lys Gly Lys Asn Asp Asn Lys Pro

85 90 95

Leu Ser Pro Glu Thr Trp Gln His Leu Lys Gly Leu Val Thr Arg Gln

100 105 110

Leu Ser Gly Lys Arg Leu Phe Val Val Asp Ala Phe Cys Gly Ala Asn

115 120 125

Pro Asp Thr Arg Leu Ser Val Arg Phe Ile Thr Glu Val Ala Trp Gln

130 135 140

Ala His Phe Val Lys Asn Met Phe Ile Arg Pro Ser Asp Glu Glu Leu

145 150 155 160

Ala Gly Phe Lys Pro Asp Phe Ile Val Met Asn Gly Ala Lys Cys Thr

165 170 175

Asn Pro Gln Trp Lys Glu Gln Gly Leu Asn Ser Glu Asn Phe Val Ala

180 185 190

Phe Asn Leu Thr Glu Arg Met Gln Leu Ile Gly Gly Thr Trp Tyr Gly

195 200 205

Gly Glu Met Lys Lys Gly Met Phe Ser Met Met Asn Tyr Leu Leu Pro

210 215 220

Leu Lys Gly Ile Ala Ser Met His Cys Ser Ala Asn Val Gly Glu Lys

225 230 235 240

Gly Asp Val Ala Val Phe Phe Gly Leu Ser Gly Thr Gly Lys Thr Thr

245 250 255

Leu Ser Thr Asp Pro Lys Arg Arg Leu Ile Gly Asp Asp Glu His Gly

260 265 270

Trp Asp Asp Asp Gly Val Phe Asn Phe Glu Gly Gly Cys Tyr Ala Lys

275 280 285

Thr Ile Lys Leu Ser Lys Glu Ala Glu Pro Glu Ile Tyr Asn Ala Ile

290 295 300

Arg Arg Asp Ala Leu Leu Glu Asn Val Thr Val Arg Glu Asp Gly Thr

305 310 315 320

Ile Asp Phe Asp Asp Gly Ser Lys Thr Glu Asn Thr Arg Val Ser Tyr

325 330 335

Pro Ile Tyr His Ile Asp Asn Ile Val Lys Pro Val Ser Lys Ala Gly

340 345 350

His Ala Thr Lys Val Ile Phe Leu Thr Ala Asp Ala Phe Gly Val Leu

355 360 365

Pro Pro Val Ser Arg Leu Thr Ala Asp Gln Thr Gln Tyr His Phe Leu

370 375 380

Ser Gly Phe Thr Ala Lys Leu Ala Gly Thr Glu Arg Gly Ile Thr Glu

385 390 395 400

Pro Thr Pro Thr Phe Ser Ala Cys Phe Gly Ala Ala Phe Leu Ser Leu

405 410 415

His Pro Thr Gln Tyr Ala Glu Val Leu Val Lys Arg Met Gln Ala Ala

420 425 430

Gly Ala Gln Ala Tyr Leu Val Asn Thr Gly Trp Asn Gly Thr Gly Lys

435 440 445

Arg Ile Ser Ile Lys Asp Thr Arg Ala Ile Ile Asp Ala Ile Leu Asn

450 455 460

Gly Ser Leu Asp Asn Ala Glu Thr Phe Thr Leu Pro Met Phe Asn Leu

465 470 475 480

Ala Ile Pro Thr Glu Leu Pro Gly Val Asp Thr Lys Ile Leu Asp Pro

485 490 495

Arg Asn Thr Tyr Ala Ser Pro Glu Gln Trp Gln Glu Lys Ala Glu Thr

500 505 510

Leu Ala Lys Leu Phe Ile Asp Asn Phe Asp Lys Tyr Thr Asp Thr Pro

515 520 525

Ala Gly Ala Ala Leu Val Ala Ala Gly Pro Lys Leu

530 535 540

<210> 183

<211> 539

<212> PRT

<213> Serratia grimesii NBRC13537

<400> 183

Met Arg Val Lys Gly Ile Thr Pro Gln Asp Leu Ala Ala Tyr Gly Ile

1 5 10 15

His Asn Val Gly Glu Ile Val His Asn Pro Ser Tyr Asp Leu Leu Phe

20 25 30

Lys Glu Glu Thr Asp Pro Ser Leu Glu Gly Phe Glu Arg Gly Val Val

35 40 45

Thr Lys Leu Gly Ala Val Ser Val Asp Thr Gly Ile Phe Thr Gly Arg

50 55 60

Ser Pro Lys Asp Lys Tyr Ile Val Arg Asp Asp Ile Thr Arg Asp Thr

65 70 75 80

Val Trp Trp Ala Asp Gln Gly Lys Gly Lys Asn Asp Asn Lys Pro Leu

85 90 95

Ser Pro Glu Ile Trp Ala Asp Leu Lys Gln Leu Val Thr Thr Gln Leu

100 105 110

Ser Gly Lys Arg Leu Phe Val Met Asp Thr Phe Cys Gly Ala Asn Ala

115 120 125

Asp Ser Arg Leu Lys Val Arg Phe Ile Thr Glu Val Ala Trp Gln Ala

130 135 140

His Phe Val Lys Asn Met Phe Ile Arg Pro Thr Asp Glu Glu Leu Gln

145 150 155 160

Asp Phe Glu Pro Asp Phe Val Val Met Asn Gly Ala Lys Cys Thr Asn

165 170 175

Pro Asn Trp Gln Gln Gln Gly Leu Asn Ser Glu Asn Phe Val Ala Phe

180 185 190

Asn Leu Thr Glu Arg Met Gln Leu Ile Gly Gly Thr Trp Tyr Gly Gly

195 200 205

Glu Met Lys Lys Gly Met Phe Ser Ile Met Asn Tyr Leu Leu Pro Leu

210 215 220

Lys Gly Ile Ala Ser Met His Cys Ser Ala Asn Val Gly Glu Lys Gly

225 230 235 240

Asp Val Ala Val Phe Phe Gly Leu Ser Gly Thr Gly Lys Thr Thr Leu

245 250 255

Ser Thr Asp Pro Lys Arg Gln Leu Ile Gly Asp Asp Glu His Gly Trp

260 265 270

Asp Asp Asp Gly Val Phe Asn Phe Glu Gly Gly Cys Tyr Ala Lys Thr

275 280 285

Ile Lys Leu Ser Glu Glu Ala Glu Pro Asp Ile Tyr His Ala Ile Lys

290 295 300

Arg Asp Ala Leu Leu Glu Asn Val Val Val Leu Ala Asp Gly Thr Ile

305 310 315 320

Asp Phe Asn Asp Gly Ser Lys Thr Glu Asn Thr Arg Val Ser Tyr Pro

325 330 335

Ile Tyr His Ile Gln Asn Ile Val Lys Pro Val Ser Lys Ala Gly His

340 345 350

Ala Thr Lys Val Ile Phe Leu Thr Ala Asp Ala Phe Gly Val Leu Pro

355 360 365

Pro Val Ser Arg Leu Thr Ala Asn Gln Thr Gln Tyr His Phe Leu Ser

370 375 380

Gly Phe Thr Ala Lys Leu Ala Gly Thr Glu Arg Gly Val Thr Glu Pro

385 390 395 400

Thr Pro Thr Phe Ser Ala Cys Phe Gly Ala Ala Phe Leu Ser Leu His

405 410 415

Pro Thr Gln Tyr Ala Glu Val Leu Val Lys Arg Met Gln Ala Ala Gly

420 425 430

Ala Gln Ala Tyr Leu Val Asn Thr Gly Trp Asn Gly Thr Gly Lys Arg

435 440 445

Ile Ser Ile Lys Asp Thr Arg Gly Ile Ile Asp Ala Ile Leu Ser Gly

450 455 460

Glu Ile Asp Lys Ala Glu Thr Ile Thr Leu Pro Ile Phe Asp Leu Ala

465 470 475 480

Met Pro Thr Ser Leu Pro Gly Val Asn Pro Asp Ile Leu Asp Pro Arg

485 490 495

Ala Thr Tyr Ala Ser Leu Glu Gln Trp Gln Glu Lys Ala Gln Asp Leu

500 505 510

Ala Glu Arg Phe Val Thr Asn Phe Asp Lys Tyr Thr Asp Thr Pro Ala

515 520 525

Gly Ala Ala Leu Val Ser Ala Gly Pro Lys Leu

530 535

<210> 184

<211> 539

<212> PRT

<213> Serratia grimesii NBRC13537

<400> 184

Met Ser Ser Ile Ala Ile Thr Pro Gln Thr Leu Ala Arg Tyr Gly Ile

1 5 10 15

His Gln Ala Thr Glu Ile Val His Asn Pro Ser Tyr Glu Gln Leu Phe

20 25 30

Thr Glu Glu Thr Arg Ala Asp Leu Pro Ala Leu Glu Arg Gly Thr Leu

35 40 45

Thr Thr Leu Gly Ala Val Asn Val Asp Thr Gly Glu Phe Thr Gly Arg

50 55 60

Ser Pro Lys Asp Lys Tyr Ile Val Arg Asp Asp Thr Thr Arg Asp Thr

65 70 75 80

Val Trp Trp Ser Asp Asn Gly Thr Gly Lys Asn Asp Asn Gln Pro Leu

85 90 95

Ser Pro Glu Ile Trp Gln His Leu Lys Thr Leu Val Gly Asn Gln Leu

100 105 110

Ser Gly Lys Arg Leu Phe Val Val Asp Ala Phe Cys Gly Ala Asn Ala

115 120 125

Asp Thr Arg Leu Lys Val Arg Phe Ile Thr Glu Val Ala Trp Gln Ala

130 135 140

His Phe Val Lys Asn Met Phe Ile Arg Pro Thr Asp Glu Glu Leu Gln

145 150 155 160

Asn Phe Glu Pro Asp Phe Val Val Met Asn Gly Ala Lys Cys Thr Asn

165 170 175

Pro Asp Trp Gln Gln Gln Gly Met His Ser Glu Asn Phe Val Thr Phe

180 185 190

Asn Leu Thr Glu Arg Met Gln Leu Ile Gly Gly Thr Trp Tyr Gly Gly

195 200 205

Glu Met Lys Lys Gly Leu Phe Ser Ile Met Asn Tyr Leu Leu Pro Leu

210 215 220

Gln Gly Ile Ala Ser Met His Cys Ser Ala Asn Val Gly Glu Gln Gly

225 230 235 240

Asp Val Ala Val Phe Phe Gly Leu Ser Gly Thr Gly Lys Thr Thr Leu

245 250 255

Ser Thr Asp Pro Lys Arg Gln Leu Ile Gly Asp Asp Glu His Gly Trp

260 265 270

Asp Asp Asp Gly Val Phe Asn Phe Glu Gly Gly Cys Tyr Ala Lys Thr

275 280 285

Ile Lys Leu Ser Gln Gln Ala Glu Pro Glu Ile Tyr Gln Ala Ile Arg

290 295 300

Arg Asn Ala Leu Leu Glu Asn Val Gln Val Leu Ala Asp Gly Ser Ile

305 310 315 320

Asp Phe Asp Asp Ala Ser Lys Thr Glu Asn Thr Arg Val Ser Tyr Pro

325 330 335

Ile Asp His Ile Glu Asn Ile Val Lys Pro Val Ser Lys Ala Gly His

340 345 350

Ala Lys Lys Ile Ile Phe Leu Thr Ala Asp Ala Phe Gly Val Leu Pro

355 360 365

Pro Val Ser Arg Leu Thr Pro Glu Gln Thr Gln Tyr His Phe Leu Ser

370 375 380

Gly Phe Thr Ala Lys Leu Ala Gly Thr Glu Arg Gly Ile Thr Thr Pro

385 390 395 400

Thr Pro Thr Phe Ser Ala Cys Phe Gly Ala Ala Phe Leu Thr Leu His

405 410 415

Pro Thr Gln Tyr Ala Glu Val Leu Val Lys Arg Met Glu Ala Ala Gly

420 425 430

Ala Gln Ala Tyr Leu Val Asn Thr Gly Trp Asn Gly Ser Gly Lys Arg

435 440 445

Ile Ser Ile Lys Asp Thr Arg Gly Ile Ile Asp Ala Ile Leu Asn Gly

450 455 460

Glu Ile Glu His Ala Glu Thr Phe Thr Leu Pro Ile Phe Gly Leu Ala

465 470 475 480

Val Pro Thr Ala Leu Pro Gly Val Asp Pro Ser Ile Leu Asp Pro Arg

485 490 495

Lys Thr Tyr Ala Asp Glu Ser Leu Trp Gln Glu Lys Ala Gln Asp Leu

500 505 510

Ala Gln Arg Phe Ile Asp Asn Phe Ala Lys Tyr Thr Val Thr Pro Ala

515 520 525

Gly Glu Lys Leu Val Gly Ala Gly Pro Lys Leu

530 535

<210> 185

<211> 477

<212> PRT

<213> Serratia grimesii NBRC13537

<400> 185

Met Phe Lys Asn Ala Phe Ala Asn Leu Gln Lys Val Gly Lys Ser Leu

1 5 10 15

Met Leu Pro Val Ser Val Leu Pro Ile Ala Gly Ile Leu Leu Gly Val

20 25 30

Gly Ser Ala Asn Phe Ser Trp Leu Pro Ala Val Val Ser His Val Met

35 40 45

Ala Glu Ala Gly Gly Ser Val Phe Ala Asn Met Pro Leu Ile Phe Ala

50 55 60

Ile Gly Val Ala Leu Gly Phe Thr Asn Asn Asp Gly Val Ser Ala Leu

65 70 75 80

Ala Ala Val Val Ala Tyr Gly Ile Met Val Lys Thr Met Ala Val Val

85 90 95

Ala Pro Leu Val Leu His Leu Pro Ala Glu Glu Ile Ala Ala Lys His

100 105 110

Leu Ala Asp Thr Gly Val Leu Gly Gly Ile Ile Ser Gly Ser Ile Ala

115 120 125

Ala Tyr Met Phe Asn Arg Phe Phe Arg Ile Gln Leu Pro Glu Tyr Leu

130 135 140

Gly Phe Phe Ala Gly Lys Arg Phe Val Pro Ile Ile Ser Gly Leu Ala

145 150 155 160

Ala Ile Val Leu Gly Val Val Leu Ser Phe Ile Trp Pro Pro Ile Gly

165 170 175

Thr Ala Ile Gln Thr Phe Ser Gln Trp Ala Ala Tyr Gln Asn Pro Val

180 185 190

Val Ala Phe Gly Ile Tyr Gly Val Val Glu Arg Ala Leu Val Pro Phe

195 200 205

Gly Leu His His Ile Trp Asn Val Pro Phe Gln Met Gln Ile Gly Glu

210 215 220

Tyr Thr Asn Ala Ala Gly Gln Val Phe His Gly Asp Ile Pro Arg Tyr

225 230 235 240

Met Ala Gly Asp Pro Thr Ala Gly Lys Leu Ser Gly Gly Phe Leu Phe

245 250 255

Lys Met Tyr Gly Leu Pro Ala Ala Ala Ile Ala Ile Trp His Ser Ala

260 265 270

Lys Pro Glu Asn Arg Ala Lys Val Gly Gly Ile Met Ile Ser Ala Ala

275 280 285

Leu Thr Ser Phe Leu Thr Gly Ile Thr Glu Pro Ile Glu Phe Ser Phe

290 295 300

Met Phe Val Ala Pro Ile Leu Tyr Ala Ile His Ala Ile Leu Ala Gly

305 310 315 320

Leu Ala Phe Pro Ile Cys Ile Leu Leu Gly Met Arg Asp Gly Thr Ser

325 330 335

Phe Ser His Gly Leu Ile Asp Phe Ile Val Leu Ser Gly Asn Ser Ser

340 345 350

Lys Ile Trp Leu Phe Pro Ile Val Gly Ile Ile Tyr Gly Leu Val Tyr

355 360 365

Tyr Thr Ile Phe Arg Val Leu Ile Ala Lys Leu Asp Leu Lys Thr Pro

370 375 380

Gly Arg Glu Asp Thr Val Ser Glu Gln Val Ala Gln Gly Gly Ser Glu

385 390 395 400

Met Ser Ala Ala Leu Val Gln Ala Phe Gly Gly Lys Glu Asn Ile Thr

405 410 415

Asn Leu Asp Ala Cys Ile Thr Arg Leu Arg Val Ser Val Ala Asp Val

420 425 430

Ser Lys Val Asp Gln Ala Gly Leu Lys Lys Leu Gly Ala Ala Gly Val

435 440 445

Val Val Ala Gly Ser Gly Val Gln Ala Ile Phe Gly Thr Lys Ser Asp

450 455 460

Asn Leu Lys Thr Asp Met Asp Glu Tyr Ile Arg Asn His

465 470 475

<210> 186

<211> 200

<212> DNA

<213> Escherichia coli str. K-12 substr. MG1655

<400> 186

cgtaattgcc ctttaaaatt cggggcgccg accccatgtg gtctcaagcc caaaggaaga 60

gtgaggcgag tcagtcgcgt aatgcttagg cacaggattg atttgtcgca atgattgaca 120

cgattccgct tgacgctgcg taaggttttt gtaattttac aggcaacctt ttattcacta 180

acaaatagct ggtggaatat 200

<210> 187

<211> 30

<212> DNA

<213> Artificial Sequence

<220>

<223> primer

<400> 187

taccgtcgac ctcgacgtaa ttgcccttta 30

<210> 188

<211> 41

<212> DNA

<213> Artificial Sequence

<220>

<223> primer

<400> 188

ggccccccct cgagtcatta agtactatat tccaccagct a 41

<210> 189

<211> 774

<212> DNA

<213> Pseudomonas putida KT2440

<400> 189

atgccgcgat atatcgatgt gcaggcgccc gaacatggcg ttcagctcat taccctgcaa 60

cggcccgagg ccttgaatgc cctgtgcacc gagctactgg cagaactggc cgctgcgctg 120

caggctgccg ggaacgacga gcatgtccgt gccacagtga ttaccggcag cgccaaggca 180

ttcgccgcag gcgccgacat ccgcgagatg gccgatcgcg acctggtcgg catcctcaat 240

gacccgcgcg tagcgcattg gcaaagcatc gccgcattcg ccaaaccgct gattgctgca 300

gtcaacggct atgccctggg tggcggttgc gaactggcaa tgtgcgccga catcgtcatc 360

gccagtaccg acgcccgttt cggccagccg gaaatcaacc ttggcatcat ccccggtgct 420

ggcggcaccc agcgcctgtt acgtgccgtc ggtaagccgt tggccatgca gatggtgctg 480

acgggggaag ccatcactgc cctccgcgcc cagcaggccg gcctggtcag cgaaatcacc 540

cagcccgaac tcaccgtaga acgcgccatg caggttgccc gcagcatcgc cgccaaagcg 600

ccgctggctg tgcgcctggc caaggaggcg ttactgaagg ccggtgatac cgacctggcc 660

agcggcctgc gcttcgagcg ccatgccttc accctgctgg cgggcaccgc cgaccgcgat 720

gaaggcatcc gcgccttcca ggaaaagcgc caggcccgct tccaagggcg ctga 774

<210> 190

<211> 32

<212> DNA

<213> Artificial Sequence

<220>

<223> primer

<400> 190

ctggtggaat atatgcacga cgtattcatc tg 32

<210> 191

<211> 41

<212> DNA

<213> Artificial Sequence

<220>

<223> primer

<400> 191

ctcgagtcat taagtgttaa ctcaaacccg ctcgatggcc a 41

<210> 192

<211> 696

<212> DNA

<213> Pseudomonas putida KT2440

<400> 192

ttgatcaata aaacgtacga gtccatcgcc agcgcggtgg aagggattac cgacggttcg 60

accatcatgg tcggtggctt cggcacggct ggcatgccgt ccgagctgat cgatggcctc 120

attgccaccg gtgcccgcga cctgaccatc atcagcaaca acgccggcaa cggcgagatc 180

ggcctggccg ccctgctcat ggcaggcagc gtgcgcaagg tggtctgctc gttcccgcgc 240

cagtccgact cctacgtgtt cgacgaactg taccgcgccg gcaagatcga gctggaagtg 300

gtcccgcagg gcaacctggc cgagcgtatc cgcgccgcag gctccggcat tggtgcgttc 360

ttctcgccaa ccggctacgg caccctgctg gccgagggca aggaaacccg tgagatcgat 420

ggccgcatgt acgtgctgga aatgccgctg cacgccgact tcgcactgat caaggcgcac 480

aagggtgacc gttggggcaa cctgacctac cgcaaggccg cccgcaactt cggcccgatc 540

atggccatgg ctgccaagac cgccatcgcc caggtcgacc aggtcgtcga actcggtgaa 600

ctggacccgg aacacatcat caccccgggt atcttcgtcc agcgcgtggt cgccgtcacc 660

ggtgctgccg cttcttcgat tgccaaagct gtctga 696

<210> 193

<211> 642

<212> DNA

<213> Pseudomonas putida KT2440

<400> 193

atgaccatca ccaaaaagct ctcccgcacc gagatggccc aacgcgtggc cgcagacatc 60

caggaaggcg cgtacgtaaa cctgggcatc ggcgcaccga ccctggtggc caactacctg 120

ggcgacaagg aagtgttcct gcacagcgag aacggcctgc tgggcatggg cccaagccct 180

gcgccgggcg aggaagacga tgacctgatc aacgccggca agcagcacgt caccctgctg 240

accggtggtg ccttcttcca ccatgccgat tcgttctcga tgatgcgtgg cggccacctg 300

gacatcgctg tactgggcgc cttccaggtg tcggtcaagg gcgacctggc caactggcac 360

acgggtgccg aaggctcgat cccggccgta ggcggtgcaa tggacctggc caccggcgcc 420

cgccaggtgt tcgtgatgat ggaccacctg accaagaccg gcgaaagcaa gctggtgccc 480

gagtgcacct acccgctgac cggtatcgct tgcgtcagcc gcatctacac cgacctggcc 540

gtactggaag tgacacctga agggctgaaa gtggtcgaaa tctgcgcgga catcgacttt 600

gacgagctgc agaaactcag tggcgtgccg ctgatcaagt ga 642

<210> 194

<211> 55

<212> DNA

<213> Artificial Sequence

<220>

<223> primer

<400> 194

gagcgggttt gagttcaatt gtagctggtg gaatatttga tcaataaaac gtacg 55

<210> 195

<211> 34

<212> DNA

<213> Artificial Sequence

<220>

<223> primer

<400> 195

gagtcattaa gtgtttcact tgatcagcgg cacg 34

<210> 196

<211> 35

<212> DNA

<213> Artificial Sequence

<220>

<223> primer

<400> 196

ctggtggaat atagtactat ggcagaaagt aatgc 35

<210> 197

<211> 39

<212> DNA

<213> Artificial Sequence

<220>

<223> primer

<400> 197

ttccaccagc taagtgctag cttaaggcgt ggtcgtcag 39

<210> 198

<211> 35

<212> DNA

<213> Artificial Sequence

<220>

<223> primer

<400> 198

ctggtggaat atagtactat ggcagaaagt aatgc 35

<210> 199

<211> 39

<212> DNA

<213> Artificial Sequence

<220>

<223> primer

<400> 199

ttccaccagc taagtgctag cttaaggcgt ggtcgtcag 39

<210> 200

<211> 35

<212> DNA

<213> Artificial Sequence

<220>

<223> primer

<400> 200

ctggtggaat atagtactat ggcagagaat aattc 35

<210> 201

<211> 39

<212> DNA

<213> Artificial Sequence

<220>

<223> primer

<400> 201

ttccaccagc taagtgctag cttagggcgc tacagccag 39

<210> 202

<211> 35

<212> DNA

<213> Artificial Sequence

<220>

<223> primer

<400> 202

ctggtggaat atagtactat ggcagagaat aattc 35

<210> 203

<211> 38

<212> DNA

<213> Artificial Sequence

<220>

<223> primer

<400> 203

ttccaccagc taagtgctag cttagggcgc gacggcca 38

<210> 204

<211> 35

<212> DNA

<213> Artificial Sequence

<220>

<223> primer

<400> 204

ctggtggaat atagtactat ggcagaaagt aatgc 35

<210> 205

<211> 40

<212> DNA

<213> Artificial Sequence

<220>

<223> primer

<400> 205

ttccaccagc taagtgctag cttaaggcgt ggtggtcagc 40

<210> 206

<211> 35

<212> DNA

<213> Artificial Sequence

<220>

<223> primer

<400> 206

ctggtggaat atagtactat ggcagagaat aattc 35

<210> 207

<211> 40

<212> DNA

<213> Artificial Sequence

<220>

<223> primer

<400> 207

ttccaccagc taagtgctag cttacggcgc gacggccagt 40

<210> 208

<211> 35

<212> DNA

<213> Artificial Sequence

<220>

<223> primer

<400> 208

ctggtggaat atagtactat ggcagagaat aatac 35

<210> 209

<211> 40

<212> DNA

<213> Artificial Sequence

<220>

<223> primer

<400> 209

ttccaccagc taagtgctag ctcatggcgc gacggccagc 40

<210> 210

<211> 40

<212> DNA

<213> Artificial Sequence

<220>

<223> primer

<400> 210

cggccagtga attcgagctc cgtaattgcc ctttaaaatt 40

<210> 211

<211> 41

<212> DNA

<213> Artificial Sequence

<220>

<223> primer

<400> 211

gatccccggg taccgcatgc tatattccac cagctatttg t 41

<210> 212

<211> 34

<212> DNA

<213> Artificial Sequence

<220>

<223> primer

<400> 212

gctggtggaa tatagatgcc gcgatatatc gatg 34

<210> 213

<211> 34

<212> DNA

<213> Artificial Sequence

<220>

<223> primer

<400> 213

attacgccaa gcttgtcagc gcccttggaa gcgg 34

<210> 214

<211> 1155

<212> DNA

<213> Acinetobacter baylyi ADP1 dcaA

<400> 214

atgattcgcg atgaagggat gttgcaacaa ttactttcga caatacgaga ttttgtaaaa 60

aatgaattga ttcctcgaga gcatgaagtt gcagaaaagg attgtattcc tgaagatatt 120

attcagcaaa tgcgagaact aggcctattt ggtttaacca ttcccgaaga atacggtgga 180

cttggaatca caatggaaga agaggtgaat gtcgcgtttg aacttggtca gacatcccca 240

gcatttcgtt cattgattgg cacaaataat ggcattggtt caagtggttt aatcattgat 300

ggaactgagg agcaaaaaca gaaatatttg ccacgttatg ccagtggaga aattattggt 360

tcattttgct taactgaacc agaagcgggt tcagatgctg cctctttaaa aacgacagcg 420

gtaaaagatg gtgatttcta catattaaat ggaaccaagc gttttattac caatgcaccg 480

catgcagcaa catttaccgt aatggcacgc accaatccag caattaaagg ggcaggtgga 540

atttctgctt ttttggtaga agccaataca ccaggtatca cactaggcaa aatagatcag 600

aaaatgggac aaaaaggctc tcatacctgt gatgtgattt ttgaaaattg tcgagtacct 660

gcatctgcat taattggtgg cgttgaaggc gttggtttta aaacagcaat gaaagtgctg 720

gataaaggcc gtctacatat tggtgcatat agtgtaggtg ttgcagagcg tatgttgaat 780

gatgcactac attatgctgt cgagcgtaag cagtttggtc aacccattgc aaattttcaa 840

ttgattcaag ccatgctggc agactctaaa gccgaaattt atgcggctaa atgtatggtt 900

ttggatgcag cacgtcgccg tgatgaaggc caaaatatta gtacagaagc ctcatgtgcc 960

aagatgtttg caacagaaat gtgtggtcga gtcgcagatc gctgtgtgca aattcatggt 1020

ggggcaggtt acatcagtga atattcgatt gagcgttttt atcgtgacgt gcgtttattc 1080

cgtctttatg agggtacgac ccaagttcag caaattatta ttgccaaaaa tatgattaag 1140

gaagtgacgt cctaa 1155

<210> 215

<211> 50

<212> DNA

<213> Artificial Sequence

<220>

<223> primer

<400> 215

gggcgctgac aagcttagct ggtggaatat atgattcgcg atgaagggat 50

<210> 216

<211> 36

<212> DNA

<213> Artificial Sequence

<220>

<223> primer

<400> 216

tgattacgcc aagcttttag gacgtcactt ccttaa 36

<210> 217

<211> 34

<212> DNA

<213> Artificial Sequence

<220>

<223> primer

<400> 217

tatagggcga attggcgtaa ttgcccttta aaat 34

<210> 218

<211> 40

<212> DNA

<213> Artificial Sequence

<220>

<223> primer

<400> 218

ccgccaccgc ggtggagctc atattccacc agctatttgt 40

<210> 219

<211> 1620

<212> DNA

<213> Serratia grimesii NBRC13537

<400> 219

atgcgtgtta aaggtataac ccctcaagat ctggccgctt atgggattca taacgttggc 60

gaaatcgttc acaacccaag ttatgacttg ctgtttaagg aagaaacaga cccatctctg 120

gaaggttttg agcgtggggt cgtaaccaag ctgggtgctg tatccgtcga caccggtatt 180

tttaccggcc gttccccgaa agataagtac atcgtccgtg acgacatcac ccgcgacacc 240

gtctggtggg ccgatcaggg taaaggtaaa aacgataata aaccccttag cccggaaatt 300

tgggcagacc tgaaacaact ggtcaccacg caactctccg gcaaacgcct gtttgtgatg 360

gataccttct gcggtgccaa tgctgactcc cgtctgaaag tccgctttat taccgaggtc 420

gcctggcagg cacacttcgt taaaaacatg ttcatccgtc cgaccgacga agaactgcag 480

gatttcgagc cagacttcgt tgtcatgaac ggcgccaaat gcactaaccc gaactggcag 540

caacagggcc tgaactcgga aaactttgtc gccttcaacc tgaccgaacg catgcaactg 600

attggcggta cctggtacgg cggcgaaatg aaaaagggta tgttctccat catgaactac 660

ctgttgccgc tgaaaggcat tgcctccatg cactgctcgg ccaacgtggg cgagaaaggc 720

gacgtggcgg tattcttcgg cctgtccggc accggcaaaa ccaccctgtc caccgatccg 780

aaacgccaac tgattggcga tgacgaacac ggctgggatg acgacggtgt gtttaacttc 840

gaaggcggct gctacgcgaa aaccatcaag ctgtctgaag aagccgaacc ggatatctat 900

cacgccatca aacgcgatgc gctactggaa aacgttgttg ttctggctga cggcacaata 960

gatttcaatg atggttcgaa aaccgagaac acccgtgttt cctacccgat ctatcacatc 1020

cagaatatcg taaaaccggt ttccaaagcg ggtcacgcca ccaaggtgat cttcctgacc 1080

gcagacgctt tcggtgtact gccgccggta tctcgcctga ccgctaacca gacccagtac 1140

cacttcctgt ctggctttac cgccaaactg gccggtaccg agcgcggcgt gacagagcct 1200

accccaacct tctccgcctg cttcggcgca gccttcctgt cactgcaccc aacgcagtac 1260

gctgaagtgc tggtgaaacg catgcaagct gccggcgcac aggcctatct ggtgaacacc 1320

ggctggaacg gcaccggcaa acgcatctcg atcaaggata cccgcggcat tatcgacgcg 1380

atcctcagcg gtgaaatcga taaggcggaa accattactc tgcctatctt cgatctggcg 1440

atgccaacct cactgccggg tgttaacccg gatatcctgg atccacgcgc cacttatgcc 1500

agccttgagc aatggcaaga gaaggcacag gatctggccg agcgttttgt caccaacttt 1560

gacaaataca ccgatacccc ggcaggtgcc gcgttggtca gcgctggtcc aaaactgtaa 1620

<210> 220

<211> 34

<212> DNA

<213> Artificial Sequence

<220>

<223> primer

<400> 220

agctggtgga atatgatgcg tgttaaaggt ataa 34

<210> 221

<211> 40

<212> DNA

<213> Artificial Sequence

<220>

<223> primer

<400> 221

ccgccaccgc ggtggagctc ttacagtttt ggaccagcgc 40

<210> 222

<211> 70

<212> DNA

<213> Artificial Sequence

<220>

<223> primer

<400> 222

gctggtttta ttcgtttctt tatccatcac aacttgtaga aaaatccgtc gtgtaggctg 60

gagctgcttc 70

<210> 223

<211> 70

<212> DNA

<213> Artificial Sequence

<220>

<223> primer

<400> 223

aaacgccgca aagcggcgtt tttgacaaaa gaattatgtc acgttttata atgggaatta 60

gccatggtcc 70

<210> 224

<211> 20

<212> DNA

<213> Artificial Sequence

<220>

<223> primer

<400> 224

cggtgccctg aatgaactgc 20

<210> 225

<211> 20

<212> DNA

<213> Artificial Sequence

<220>

<223> primer

<400> 225

cgccggtgaa accgtcacga 20

<210> 226

<211> 20

<212> DNA

<213> Artificial Sequence

<220>

<223> primer

<400> 226

gtgctgcgtc gtctttagct 20

<210> 227

<211> 70

<212> DNA

<213> Artificial Sequence

<220>

<223> primer

<400> 227

gacgttatcg caacaccaat aatttgcttc accagtcaac ggagtaatac gtgtaggctg 60

gagctgcttc 70

<210> 228

<211> 70

<212> DNA

<213> Artificial Sequence

<220>

<223> primer

<400> 228

cagtcaggtt cagtgtgtgc tcatgacgtg acatggcgaa gataggcagg atgggaatta 60

gccatggtcc 70

<210> 229

<211> 20

<212> DNA

<213> Artificial Sequence

<220>

<223> primer

<400> 229

tggtggaaat ggcgcagctt 20

<210> 230

<211> 20

<212> DNA

<213> Artificial Sequence

<220>

<223> primer

<400> 230

gcgtaaccga cccaattcga 20

<210> 231

<211> 67

<212> DNA

<213> Artificial Sequence

<220>

<223> primer

<400> 231

gaaagcaagt ttctcccatc cttctcaact taaagactaa gactgtcgtg taggctggag 60

ctgcttc 67

<210> 232

<211> 70

<212> DNA

<213> Artificial Sequence

<220>

<223> primer

<400> 232

cgcccatcag ggcgcttcga tatacaaatt aattcacaaa agcaatatta atgggaatta 60

gccatggtcc 70

<210> 233

<211> 18

<212> DNA

<213> Artificial Sequence

<220>

<223> primer

<400> 233

ccggatgaac tttcactt 18

<210> 234

<211> 18

<212> DNA

<213> Artificial Sequence

<220>

<223> primer

<400> 234

gagctgcgtc atctttag 18

<210> 235

<211> 70

<212> DNA

<213> Artificial Sequence

<220>

<223> primer

<400> 235

ggatttcatg ttcaagcaac acctggttgt ttcagtcaac ggagtattac gtgtaggctg 60

gagctgcttc 70

<210> 236

<211> 70

<212> DNA

<213> Artificial Sequence

<220>

<223> primer

<400> 236

tgaactgtag gccggatgtg gcgttttcgc cgcatccggc aacgtactta atgggaatta 60

gccatggtcc 70

<210> 237

<211> 18

<212> DNA

<213> Artificial Sequence

<220>

<223> primer

<400> 237

acgcatgagt tgtatgaa 18

<210> 238

<211> 18

<212> DNA

<213> Artificial Sequence

<220>

<223> primer

<400> 238

ttacccgatc aggatcat 18

<210> 239

<211> 672

<212> DNA

<213> Acinetobacter baylyi ADP1 dcaI

<400> 239

atgattaata aaatcattaa tgatattgag cccattttaa aatcgattcc agatggttca 60

acgattatga caagtggttt tggtacgaca ggtcaacctg aggcactact tgaagcatta 120

attgatttcg ctccaaaaga gttaactatt atcaataata atgcttcatc tggcccgaat 180

ggtttgactc agcttttcac tgctggattg gtcaaaaagc tcatttgctc gtatccaaaa 240

tcaatcagtt cgactgtttt tccagattta tatcgtgcgg gaaaaattga acttgaactg 300

gttcctcagg gaaatcttgc atgtcgtatt caagcagcag gtgctggtct gggagcggta 360

tttaccccca caggctacgg gaccaaaatt gctgaaggca aagaaacgcg cattattaac 420

ggtaaaaatt atgtacttga atatccactt gaagccgatt atgcattcat ttatgcagat 480

aaggcagatc gctgggggaa tttaacttat cgtaaagccg ccagaaattt tggtccgatt 540

atggccaaag ctgcaaaaac aacaattgct caagtcaatc aaactgttga attgggtgat 600

cttgatcccg aatgcattat tacacccggg atatttgtac agcatgtggt gcgattagga 660

gatataaaat ga 672

<210> 240

<211> 675

<212> DNA

<213> Acinetobacter baylyi ADP1 dcaJ

<400> 240

atgacgattc agaaaagaag tcgagaagat atcgcaatca tgatagcaaa ggatattcct 60

gatggttcgt atgtcaatct tggaattgga ttaccaaccc atgtcgcaaa atacttgcca 120

aaagataagg aaatttttct gcactcagag aatggtgtac tggcatttgg tccacctcct 180

gctgaaggtg aagaagatca ggacttagtc aatgcaggta aagagctggt gactttacta 240

tcaggtggat gttttatgca tcatggagat tcatttgaca ttatgcgtgg tggacatctg 300

gatatttgcg ttattggggc atttcaagtc gcgctcaatg gtgatttggc taactggcat 360

acaggtaaag acgatgatgt gcctgctgtt gggggagcaa tggatttggc ggttggggcc 420

aagcgtattt ttgtgtatat ggaacatacc accaaaaaag gcgaacctaa aattgttaaa 480

catctgactt atccaatcac tggtgagcag tgtgttgatc gtatttatac agatttatgt 540

acgattgagc tgaaagatgg acaggcatat gtgattgaaa tggtcgacgg attagatttt 600

gatactttgc aagccttaac tgaatgccca ttaattgatc actgtactta ttcatcgttg 660

attcagctta gataa 675

<210> 241

<211> 55

<212> DNA

<213> Artificial Sequence

<220>

<223> primer

<400> 241

gagcgggttt gagttcaatt gtagctggtg gaatatatga ttaataaaat catta 55

<210> 242

<211> 34

<212> DNA

<213> Artificial Sequence

<220>

<223> primer

<400> 242

gagtcattaa gtgttttatc taagctgaat caac 34

<210> 243

<211> 1434

<212> DNA

<213> Serratia grimesii NBRC13537

<400> 243

atgttcaaga acgcatttgc aaacctgcaa aaagtaggta agtcgctaat gctgccggtg 60

tccgtattgc ctatcgcagg tatcctgctg ggcgtcggtt ccgccaactt tagctggcta 120

cctgcggtag tctcccacgt gatggcggaa gccggcggtt ctgttttcgc caatatgccg 180

ttaattttcg ccatcggtgt tgccctgggc ttcaccaaca acgacggtgt atccgcactg 240

gctgcagtcg tggcttacgg catcatggtg aaaaccatgg cggtggttgc acctttggtg 300

ctgcacttgc cggctgaaga gattgcggcc aaacatttgg cggataccgg tgtgctcggg 360

gggattatct ccggctccat cgctgcctat atgttcaacc gcttcttccg cattcaattg 420

ccggaatacc tgggcttctt tgcaggtaag cgttttgtgc cgattatctc cggcctggcg 480

gcgatcgttc tgggcgtagt attgtccttc atctggcctc caatcggtac ggctatccag 540

accttctccc agtgggctgc ttatcagaac ccggtagtgg cgtttggtat ttacggcgtg 600

gttgaacgtg cgctggtgcc atttggtctg caccatatct ggaacgtacc gttccaaatg 660

cagattggtg agtacaccaa cgcagcaggt caggtattcc acggcgatat cccacgttat 720

atggcgggtg acccaactgc gggtaaactg tccggtggct tcctgttcaa aatgtacggt 780

ctgcctgctg ctgcgattgc catctggcac tcagccaagc cggaaaaccg cgctaaagtc 840

ggcggtatca tgatctccgc tgccttgacc tcgttcctga ccggtatcac cgaaccgatc 900

gagttctcat tcatgttcgt cgcgccgatc ctgtacgcaa tccatgccat cctggctggc 960

ctggcgttcc cgatctgtat cctgctgggt atgcgtgacg gcaccagctt ctcacacggt 1020

ttgatcgact ttatcgtact gagcggtaac agcagcaaaa tttggctgtt cccaatcgtc 1080

ggtatcatct acggtctggt gtactacacc atcttccgtg tgctgattgc caagctggat 1140

ctgaaaaccc caggtcgcga agacactgtt tctgagcagg ttgcacaggg cggttctgaa 1200

atgtctgcgg cgctggttca ggctttcggc ggtaaagaaa acatcaccaa cctggatgcc 1260

tgtatcaccc gtctgcgtgt tagcgtggcg gacgtgagca aggttgacca ggctggcctg 1320

aaaaaactgg gtgctgccgg cgtagttgtc gcaggctctg gtgttcaggc tatctttggt 1380

accaaatctg ataacctgaa aacggatatg gacgaataca tccgtaacca ttaa 1434

<210> 244

<211> 70

<212> DNA

<213> Artificial Sequence

<220>

<223> primer

<400> 244

aagaatcgcg gaaagtagca aagcactatt actcaggagc acactcaact gtgtaggctg 60

gagctgcttc 70

<210> 245

<211> 70

<212> DNA

<213> Artificial Sequence

<220>

<223> primer

<400> 245

gccaataaaa aagggaggct ttcgcctccc ttaaaactcc cctgcctgaa atgggaatta 60

gccatggtcc 70

<210> 246

<211> 20

<212> DNA

<213> Artificial Sequence

<220>

<223> primer

<400> 246

gtaccgcttg atcgcattct 20

<210> 247

<211> 19

<212> DNA

<213> Artificial Sequence

<220>

<223> primer

<400> 247

gaatatggtt acaagcgca 19

<210> 248

<211> 70

<212> DNA

<213> Artificial Sequence

<220>

<223> primer

<400> 248

cacgcgtgag aacgtaaaaa aagcacccat actcaggagc actctcaatt gtgtaggctg 60

gagctgcttc 70

<210> 249

<211> 70

<212> DNA

<213> Artificial Sequence

<220>

<223> primer

<400> 249

agccatctgg ctgccttagt ctccccaacg tcttacggat tagtggttac atgggaatta 60

gccatggtcc 70

<210> 250

<211> 20

<212> DNA

<213> Artificial Sequence

<220>

<223> primer

<400> 250

atcggttact ggtggaaact 20

<210> 251

<211> 20

<212> DNA

<213> Artificial Sequence

<220>

<223> primer

<400> 251

gtggatggga cagtcagtaa 20