具体实施方式

本发明中发现：在具有制造3-羟基己二酸、α-氢化己二烯二酸和/或己二酸的能力的微生物中，当使该微生物的丙酮酸激酶的功能受损，并且强化了磷酸烯醇丙酮酸羧化激酶的活性和对将3-氧代己二酰-CoA还原生成3-羟基己二酰-CoA的反应进行催化的酶的活性时，能够以高产率制造3-羟基己二酸、α-氢化己二烯二酸和/或己二酸。

以下，在本说明书中，有时将对将3-氧代己二酰-CoA还原生成3-羟基己二酰-CoA的反应进行催化的酶记为“3-氧代己二酰-CoA还原酶”。另外，在本说明书中，有时将磷酸烯醇丙酮酸简称为PEP、3-羟基己二酸简称为3HA、α-氢化己二烯二酸简称为HMA、己二酸简称为ADA。

在本发明中，对磷酸烯醇丙酮酸羧化激酶的活性和对将3-氧代己二酰-CoA还原生成3-羟基己二酰-CoA的反应进行催化的酶的活性进行强化是指以下方法：从宿主微生物的菌体外向菌体内导入对这些多肽进行编码的核酸；增加对该多肽进行编码的核酸的拷贝数；对该多肽的编码区域上游的启动子区域和核糖体结合序列进行修饰的方法等。这些方法可以单独进行，也可以组合进行。对导入核酸的方法没有特别地限定，可以使用在微生物内可自体复制的表达载体中将该核酸导入重组宿主微生物中的方法；或者将该核酸组装在微生物的基因组中的方法等。

导入的核酸可以为1种，也可以为多种。并且也可以使核酸的导入和表达的增强组合起来。

在将对本发明中所表达的多肽进行编码的核酸组装在表达载体或宿主微生物基因组中的情况下，该表达载体或基因组组装用核酸优选由启动子、核糖体结合序列、对所表达的多肽进行编码的核酸、转录终止序列构成。另外，也可以包含控制启动子活性的基因。

本发明中所使用的启动子只要是可以在宿主微生物中表达酶即可，没有特别地限定，例如可以列举出gap启动子、trp启动子、lac启动子、tac启动子、T7启动子等。

在本发明中，在使用表达载体导入核酸或增强多肽的表达的情况下，只要在该微生物中可自体复制即可，没有特别地限定，例如可以列举出pBBR1MCS载体、pBR322载体、pMW载体、pET载体、pRSF载体、pCDF载体、pACYC载体、以及上述载体的衍生型等。

在本发明中，在使用基因组组装用核酸导入核酸或增强多肽的表达的情况下，使用位点特异性同源重组来导入。对位点同源重组的方法没有特别地限定，例如可以列举出：使用λRed重组酶和FLP重组酶的方法(Proc NatI Acad Sci USA.2000Jun 6；97(12):6640-6645.)；使用λRed重组酶和sacB基因的方法(Biosci Biotechnol Biochem.2007Dec；71(12):2905-11.)。

表达载体或基因组组装用核酸的导入方法只要是将核酸导入在微生物中的方法即可，没有特别地限定，例如可以列举出：钙离子法(Journal of Molecular Biology,53,159(1970))；电穿孔法(NM Calvin，PC Hanawalt.J.Bacteriol,170(1988),pp.2796-2801)等。

下述路线1是表示用于生产3-羟基己二酸、α-氢化己二烯二酸和/或己二酸所需要的反应路径的例子。这里，反应A表示从乙酰-CoA和琥珀酰-CoA生成3-氧代己二酰-CoA和辅酶A的反应。反应B表示从3-氧代己二酰-CoA还原生成3-羟基己二酰-CoA的反应。反应C表示从3-羟基己二酰-CoA生成2,3-脱氢己二酰-CoA的反应。反应D表示从2,3-脱氢己二酰-CoA生成己二酰-CoA的反应。反应E表示从3-羟基己二酰-CoA生成3-羟基己二酸的反应。反应F表示从2,3-脱氢己二酰-CoA生成α-氢化己二烯二酸的反应。反应G表示从己二酰-CoA生成己二酸的反应。

[化学式1]

已知的是，在微生物具有制造3-羟基己二酸、α-氢化己二烯二酸和/或己二酸的能力的情况下，上述路线1所示的生物合成路径当中至少具有对反应A进行催化的酶。对于从3-氧代己二酰CoA生成3-羟基己二酸、α-氢化己二烯二酸或己二酸的反应而言，优选具有路线1所示的生物合成路径。即，在本发明的基因修饰微生物具有制造3-羟基己二酸的能力的情况下，作为基因修饰微生物的宿主微生物，优选具有从乙酰-CoA和琥珀酰-CoA生成3-氧代己二酰-CoA和辅酶A(反应A)的能力；从3-氧代己二酰-CoA还原生成3-羟基己二酰-CoA(反应B)的能力；以及从3-羟基己二酰-CoA生成3-羟基己二酸(反应E)的能力。另外，在本发明的基因修饰微生物具有制造α-氢化己二烯二酸的能力的情况下，作为基因修饰微生物的宿主微生物，优选具有从乙酰-CoA和琥珀酰-CoA生成3-氧代己二酰-CoA和辅酶A(反应A)的能力；从3-氧代己二酰-CoA还原生成3-羟基己二酰-CoA(反应B)的能力；从3-羟基己二酰-CoA生成2,3-脱氢己二酰-CoA(反应C)的能力；以及从2,3-脱氢己二酰-CoA生成α-氢化己二烯二酸(反应F)的能力。此外，在本发明的基因修饰微生物具有制造己二酸的能力的情况下，作为基因修饰微生物的宿主微生物，优选具有从乙酰-CoA和琥珀酰-CoA生成3-氧代己二酰-CoA和辅酶A(反应A)的能力；从3-氧代己二酰-CoA还原生成3-羟基己二酰-CoA(反应B)的能力；从3-羟基己二酰-CoA生成2,3-脱氢己二酰-CoA(反应C)的能力；从2,3-脱氢己二酰-CoA生成己二酰-CoA(反应D)的能力；以及从己二酰-CoA生成己二酸(反应G)的能力。

只要具有这些生物合成路径的微生物对于宿主微生物可以使丙酮酸激酶的功能受损，并且强化了PEP羧化激酶的活性和对将3-氧代己二酰-CoA还原生成3-羟基己二酰-CoA的反应进行催化的酶的活性，则可以获得能够以高产率生产3-羟基己二酸、α-氢化己二烯二酸和/或己二酸的基因修饰微生物。

作为本身具有制造3-羟基己二酸的能力的微生物，可以列举出属于以下种属的微生物。

Escherichia fergusonii、Escherichia coli等大肠杆菌属。

Pseudomonas chlororaphis、Pseudomonas putida、Pseudomonas azotoformans、Pseudomonas chlororaphis subsp.aureofaciens等假单胞菌属。

Hafnia alvei等哈夫尼菌属。

Corynebacterium acetoacidophilum、Corynebacterium acetoglutamicum、Corynebacterium ammoniagenes、Corynebacterium glutamicum等棒状杆菌属。

Bacillus badius、Bacillus magaterium、Bacillus roseus等芽孢杆菌属。

Streptomyces vinaceus、Streptomyces karnatakensis、Streptomycesolivaceus等链霉菌属。

Cupriavidus metallidurans、Cupriavidus necator、Cupriavidus oxalaticus等贪铜菌属。

Acinetobacter baylyi、Acinetobacter radioresistens等不动杆菌属。

Alcaligenes faecalis等产碱菌属。

Nocardioides albus等类诺卡氏菌属。

Brevibacterium iodinum等短杆菌属。

Delftia acidovorans等戴尔福特菌属。

Shimwellia blattae等Shimwellia属。

Aerobacter cloacae等气杆菌属。

Rhizobium radiobacter等根瘤菌属。

Serratia grimesii、Serratia ficaria、Serratia fonticola、Serratiaodorifera、Serratia plymuthica、Serratia entomophila或Serratia nematodiphila等沙雷氏菌属。

在本发明的基因修饰微生物本身不具有制造3-羟基己二酸的能力的情况下，可以通过适当地组合对催化反应A、B、E的酶进行编码的核酸并导入微生物中，来赋予这些制造能力。

作为据推测本身具有制造α-氢化己二烯二酸的能力的微生物，可以列举出属于以下种属的微生物。

Escherichia fergusonii、Escherichia coli等大肠杆菌属。

Pseudomonas fluorescens、Pseudomonas putida、Pseudomonas azotoformans、Pseudomonas chlororaphis subsp.aureofaciens等假单胞菌属。

Hafnia alvei等哈夫尼菌属。

Bacillus badius等芽孢杆菌属。

Cupriavidus metallidurans、Cupriavidus numazuensis、Cupriavidusoxalaticus等贪铜菌属。

Acinetobacter baylyi、Acinetobacter radioresistens等不动杆菌属。

Alcaligenes faecalis等产碱菌属。

Delftia acidovorans等戴尔福特菌属。

Shimwellia blattae等Shimwellia属。

Serratia grimesii、Serratia ficaria、Serratia fonticola、Serratiaodorifera、Serratia plymuthica、Serratia entomophila或Serratia nematodiphila等沙雷氏菌属。

在本发明的基因修饰微生物本身不具有制造α-氢化己二烯二酸的能力的情况下，可以通过适当地组合对催化反应A、B、C、F的酶进行编码的核酸并导入微生物中，来赋予这些生成能力。

作为据推测本身具有制造己二酸的能力的微生物，可以列举出属于Thermobifidafusca等嗜热菌属的微生物。在本发明的基因修饰微生物本身不具有制造己二酸的能力的情况下，可以通过适当地组合对催化反应A、B、C、D、G的酶进行编码的核酸并导入微生物中，来赋予这些生成能力。

本发明中可以用作用于获得基因修饰微生物的宿主的微生物优选上述所列举的微生物，特别优选属于大肠杆菌属(Escherichia)、沙雷氏菌属(Serratia)、哈夫尼菌属(Hafnia)、假单胞菌属(Psuedomonas)、棒状杆菌属(Corynebacterium)、芽孢杆菌属(Bacillus)、链霉菌属(Streptomyces)、贪铜菌属(Cupriavidus)、不动杆菌属(Acinetobacter)、产碱菌属(Alcaligenes)、短杆菌属(Brevibacterium)、戴尔福特菌属(Delftia)、Shimwellia属、气杆菌属(Aerobacter)、根瘤菌属(Rhizobium)、嗜热菌属(Thermobifida)、梭菌属(Clostridium)、裂殖酵母菌属(Schizosaccharomyces)、克鲁维酵母菌属(Kluyveromyces)、毕赤酵母菌属(Pichia)以及假丝酵母菌属(Candida)的微生物。这些当中，特别优选为属于大肠杆菌属、沙雷氏菌属、哈夫尼菌属、假单胞菌属的微生物。

作为对将所述3-氧代己二酰-CoA还原生成3-羟基己二酰-CoA的反应进行催化的酶的具体例子，可以列举出以下(a)～(c)中所述的多肽。

(a)由序列号1～7的任意一者所述的氨基酸序列构成的多肽；

(b)在序列号1～7的任意一者所述的氨基酸序列中，1个或多个氨基酸发生置换、缺失、插入和/或添加后的氨基酸序列构成的、并且具有对将3-氧代己二酰-CoA还原生成3-羟基己二酰-CoA的反应进行催化的酶活性的多肽；

(c)相对于序列号1～7的任意一者所述的氨基酸序列具有70％以上的序列同源性，并且具有将3-氧代己二酰-CoA还原生成3-羟基己二酰-CoA的活性的多肽。

此外，作为3-羟基酰基-CoA脱氢酶的归类于EC1.1.1.35的酶、作为3-羟基丁酰-CoA脱氢酶的归类于EC1.1.1.157的酶也可以用作具有3-氧代己二酰-CoA还原酶活性的酶。具体而言，也可以列举出来自Pseudomonas putida KT2440株的PaaH(NCBI-Protein ID：NP745425.1)、来自Escherichia coli str.K-12substr.MG1655株的PaaH(NCBI-Protein ID：NP 415913.1)、来自Acinetobacter baylyi ADP1株的DcaH(NCBI-Protein ID：CAG68533.1)、来自Serratia plymuthica NBRC 102599株的PaaH(NCBI-Protein ID：WP063197120)、来自Serratia nematodiphila DSM 21420株的多肽(NCBI-Protein ID：WP033633399.1)作为对将3-氧代己二酰-CoA还原生成3-羟基己二酰-CoA的反应进行催化的酶。这些当中，优选上述(a)～(c)中所述的多肽。

在本发明中，对于所使用的在序列号1～7的任意一者所述的氨基酸序列中1个或多个氨基酸发生置换、缺失、插入和/或添加后的氨基酸序列构成的、并且具有3-氧代己二酰-CoA还原酶的酶活性的多肽，“1个或多个”的范围优选为10个以内、更优选为5个以内、特别优选为4个以内、最优选为1个或2个以内。这里，在氨基酸发生置换的情况下，在置换成性质类似的氨基酸的情况下(所谓的保守性置换)，多肽的活性得以保持的可能性提高。即，在氨基酸发生置换的情况下，即使置换成性质类似的氨基酸，也大多可以保持生理活性，因此置换时优选置换成性质类似的氨基酸。即，可以将构成天然蛋白质的20种氨基酸分成以下具有类似性质的组：具有低极性侧链的中性氨基酸(Gly、Ile、Val、Leu、Ala、Met、Pro)；具有亲水性侧链的中性氨基酸(Asn、Gln、Thr、Ser、Tyr、Cys)；酸性氨基酸(Asp、Glu)；碱性氨基酸(Arg、Lys、His)；芳香族氨基酸(Phe、Tyr、Trp)，如果在这些之间进行置换，则多肽的性质大多不会发生变化。

在本发明中，对于所使用的相对于序列号1～7的任意一个氨基酸序列具有70％以上的序列同源性，并且具有3-氧代己二酰-CoA还原酶的酶活性的多肽，序列同源性的优选范围优选为80％以上、更优选为85％以上、进一步优选为90％以上、进一步优选为95％以上、进一步优选为97％以上、进一步优选为99％以上。

在本发明中，“序列同源性”是指在2个氨基酸序列或碱基序列中导入间隔(Gap)或不导入间隔而排列的情况下的最佳比对(alignment)中，相同氨基酸或碱基相对于重叠(overlap)的所有氨基酸序列(包括作为翻译起始点的氨基酸)或碱基序列(包括起始密码子)的比例(百分比)，根据式(1)计算。在式(1)中，比较短的一者的序列长度为400个氨基酸以上，在小于400个氨基酸的情况下不定义为序列同源性。序列同源性可以使用本领域中通用的算法BLAST(Basic Local Alignment Search Tool)来容易地研究。例如，关于BLAST，任何人都可以从NCBI(National Center for Biotechnology Information)或KEGG(KyotoEncyclopedia of Genes and Genomes)等的网站上利用、并且使用默认参数来容易地研究序列同源性。另外，序列同源性也可以使用Genetyx等软件所携带的相同功能来研究。

序列同源性(％)＝一致数(间隔彼此不计数)/较短的序列长度(不包括两端间隔的长度)×100···式(1)。

根据式(1)，当使用Genetyx的功能(％Identity Matrix)来计算序列号1～7中所述的氨基酸序列间的序列同源性时，序列同源性的值最低的序列号2与4之间的值为71.51％，序列号1～7中所述的氨基酸序列彼此至少具有70％以上的序列同源性。使用Genetyx算出的序列同源性的结果如表1所示。需要说明的是，在下表1中，最左侧的数字表示序列号。

[表1]

将序列号1～7中所述的各氨基酸序列作为Query(查询请求)，使用BLASTP研究了其与登记在NCBI的氨基酸数据库(Non-redundant protein sequences)中的所有氨基酸序列之间的序列同源性，结果序列同源性为70％以上的序列均来自沙雷氏菌(Serratia)属细菌。

对于上述(a)的序列号1～7中所述的多肽，均在从N末端侧起第15～38个氨基酸残基(以下，为了方便，将从N末端侧起第某个氨基酸残基表示为“a.a.”。因此，例如，有时将从N末端侧起第15～38个氨基酸残基简单表示为“15～38a.a.”)中具有由序列号173所示的24个氨基酸残基构成的共通序列1。在共通序列1中，Xaa为任意的氨基酸残基，13a.a.优选为苯丙氨酸或亮氨酸，15a.a.优选为亮氨酸或谷氨酰胺，16a.a.优选为赖氨酸或天冬酰胺，17a.a.为甘氨酸或丝氨酸、更优选为甘氨酸，19a.a.优选为脯氨酸或精氨酸，21a.a.优选为亮氨酸、甲硫氨酸或缬氨酸。共通序列1对应于NAD+结合残基及其周边的氨基酸残基。如Biochimie 2012Feb；94(2):471-8.所示，NAD+结合残基的特征在于：共通序列1的第24个氨基酸残基为天冬氨酸，但是在共通序列1中为天冬酰胺。据认为：通过具有共通序列1，序列号1～7中所述的多肽表现出作为3-氧代己二酰-CoA还原酶的优异的酶活性。

对于上述(b)和(c)所述的多肽，也优选在1～200a.a.以内具有由序列号173所示的24个氨基酸残基构成的共通序列1。共通序列的位置更优选在1～150a.a.以内，进一步优选在1～100a.a.以内。作为其具体例子，可以列举出序列号8～86的氨基酸序列。序列号8～86的氨基酸序列在15～38a.a.中具有由序列号173所示的24个氨基酸残基构成的共通序列1。需要说明的是，序列号8～86的氨基酸序列与序列号1～7的任意一个氨基酸序列的序列同源性为90％以上。使用Genetyx算出的序列同源性的结果如表2-1至表2-3以及表3-1至表3-3所示。

[表2-1]

[表2-2]

[表2-3]

[表3-1]

[表3-2]

[表3-3]

对本发明的(a)～(c)中所述的多肽进行编码的核酸可以包括在该多肽的N末端和/或C末端进一步添加肽或蛋白质而得的序列。作为这种肽或蛋白质，例如可以示例出包括分泌信号序列、传输蛋白质、结合蛋白质、精制用标记肽、荧光蛋白质等。对于这种肽或蛋白质，本领域技术人员可以根据目的选择具有所添加的功能的肽或蛋白质，并将其添加到本发明的多肽中。需要说明的是，在氨基酸序列的序列同源性中不包括这种肽或蛋白质。

对序列号1～86中所述的多肽进行编码的核酸只要是可以翻译成序列号1～86中所述的氨基酸序列的碱基序列即可，没有特别地限定，可以参考对应于各氨基酸的密码子(标准遗传密码)来决定。此时，也可以使用本发明所使用的宿主微生物中常用的密码子对碱基序列再设计。

作为对具有序列号1～86中所述的氨基酸序列的多肽进行编码的核酸的碱基序列的具体例子，可以分别列举出序列号87～172中所述的碱基序列。

在本发明中，某个核酸所编码的多肽是否具有3-氧代己二酰-CoA还原酶活性是通过以下方法判断的：制作以下转化株A和转化株B，确认培养试验的结果，如果培养液中产生了3-羟基己二酸或α-氢化己二烯二酸，则该核酸对具有3-氧代己二酰-CoA还原酶活性的多肽进行编码。使用上述路线1所示的生物合成路径来说明判断方法。

转化株A具有对反应A、反应E以及反应F进行催化的酶。转化株B具有对反应A、反应C、反应E以及反应F进行催化的酶。

首先，制作转化株A。制作可以分别表达对反应A、反应E以及反应F进行催化的酶的质粒。需要说明的是，反应E和F可以使用相同的酶对反应进行催化。将该质粒导入到不具有生产3-羟基己二酸、α-氢化己二烯二酸、以及己二酸的任意一者的能力的微生物株即Escherichia coli BL21(DE3)株中。对所得的转化株导入表达质粒，从而得到转化株A，其中，所述表达质粒是将对作为研究是否具有酶活性的对象的多肽进行编码的核酸重组在适宜的启动子的下游而得的。培养转化株A，确认培养后的培养液中是否含有3-羟基己二酸。在培养液中可以确认到3-羟基己二酸的情况下，接着制作转化株B。转化株B是通过对转化株A制作并导入可以表达对反应C进行催化的酶的质粒而得到的。培养转化株B，确认培养后的培养液中是否含有α-氢化己二烯二酸。如果在培养后的培养液中可以确认到含有α-氢化己二烯二酸，则可以知道转化株A所生产的3-羟基己二酸和转化株B所生产的α-氢化己二烯二酸是经由3-羟基己二酰-CoA生成的，因此判断作为对象的多肽具有3-氧代己二酰-CoA还原酶活性。

作为对反应A进行催化的酶进行编码的基因，可以使用来自Pseudomonas putidaKT2440株的pcaF(NCBI Gene ID：1041755、序列号174)。

作为对反应E和F进行催化的酶进行编码的基因，可以使用包括来自Pseudomonasputida KT2440株的pcaI和pcaJ(NCBI Gene ID：1046613、1046612，序列号175、176)的全长的连续序列。pcaI和pcaJ所编码的多肽通过形成复合体来对反应E和反应F进行催化。

作为对反应C进行催化的酶进行编码的核酸，可以使用Pseudomonas putidaKT2440株基因paaF(NCBI Gene ID：1046932，序列号177)。

转化株A、转化株B的培养方法如下所述。需要说明的是，在培养时可以适当地添加用于稳定地保持质粒的抗生素、和诱导重组核酸所编码的多肽的表达的物质。将一白金环的转化株A或B接种在5mL的调整为pH7的培养基I(10g/L的细菌用胰蛋白胨(Bacto-tryptone，Difco Laboratories公司制造)、5g/L的细菌用酵母提取物(DifcoLaboratories公司制造)、5g/L的氯化钠)中，在30℃、120min-1下振动培养18小时，得到预培养液。接着，将0.25mL的该预培养液添加到5mL的调整为pH6.5的培养基II(10g/L的琥珀酸、10g/L的葡萄糖、1g/L的硫酸铵、50mM的磷酸钾、0.025g/L的硫酸镁、0.0625mg/L的硫酸铁、2.7mg/L的硫酸锰、0.33mg/L的氯化钙、1.25g/L的氯化钠、2.5g/L的细菌用胰蛋白胨、1.25g/L的细菌用酵母提取物)中，在30℃、120min-1下振动培养24小时。确认在所得的培养液中是否含有3-羟基己二酸或α-氢化己二烯二酸。

通过将培养液离心，并利用LC-MS/MS对上清液进行分析来确认培养液中含有3-羟基己二酸或α-氢化己二烯二酸。分析的条件如下所述。

·HPLC：1290Infinity(Agilent Technologies公司制造)

柱子：Synergi hydro-RP(Phenomenex公司制造)，长度100mm，内径3mm，粒径2.5μm

流动相：0.1％甲酸水溶液/甲醇＝70/30

流速：0.3mL/分钟

柱温：40℃

LC检测器：DAD(210nm)

·MS/MS：Triple-Quad LC/MS(Agilent Technologies公司制造)

电离法：ESI负模式。

以从3-氧代己二酸通过进行酶反应而制备的3-氧代己二酰-CoA作为基质，测定使用精制3-氧代己二酰-CoA还原酶生成的3-羟基己二酰-CoA，来计算3-氧代己二酰-CoA还原酶的活性值。具体方法如下所述。

3-氧代己二酸可以通过公知的方法(例如，WO2017/099209的参考例1中所述的方法)来制备。

3-氧代己二酰-CoA溶液的制备：按照常规方法，以Pseudomonas putida KT2440株的基因组DNA为模板进行PCR，对对CoA转移酶进行编码的核酸(pcaI和pcaJ，NCBI-Gene ID：1046613和1046612)的全长进行扩增。需要说明的是，该PCR中所使用的引物的碱基序列例如为序列号194和195。将该扩增片段插入到作为大肠菌用表达载体的pRSF-1b(Novagen公司制造)的KpnI位点上，使其成为与组氨酸标签序列相同的框架(frame)。将该质粒导入大肠菌BL 21(DE3)，按照常规方法，使用异丙基-β-硫代吡喃半乳糖苷(IPTG)诱导该酶的表达，接着利用来自培养液的组氨酸标签进行精制，得到CoA转移酶溶液。使用该溶液制备以下组成的3-氧代己二酰-CoA制备用酶反应溶液，在25℃反应3分钟后，利用UF膜(AmiconUltra-0.5mL 10K、Merck Millipore公司制造)进行处理以除去酶，并将所得的透过液作为3-氧代己二酰-CoA溶液。

(酶反应溶液)

100mM Tris-HCl(pH8.2)

10mM MgCl₂

0.5mM琥珀酰-CoA

5mM 3-氧代己二酸钠盐

2μm CoA转移酶

3-氧代己二酰-CoA还原酶活性的确认：按照常规方法，以作为对象的微生物株的基因组DNA为模板进行PCR，对对3-氧代己二酰-CoA还原酶进行编码的核酸的全长进行扩增。需要说明的是，该PCR中所使用的引物的碱基序列例如为序列号196和197。将该扩增片段插入到大肠菌用表达载体pACYCDuet-1(Novagen公司制造)的BamHI位点上，使其成为与组氨酸标签序列相同的框架。将该质粒导入大肠菌BL 21(DE3)，按照常规方法，使用异丙基-β-硫代吡喃半乳糖苷(IPTG)诱导该酶的表达，接着利用来自培养液的组氨酸标签进行精制，得到3-氧代己二酰-CoA还原酶溶液。3-氧代己二酰-CoA还原酶活性可以通过使用本酶溶液制备以下组成的酶反应溶液，测定25℃下的3-羟基己二酰-CoA的生成量来确认。

(酶反应溶液)

100mM Tris-HCl(pH8.2)

10mM MgCl₂

150μL/mL 3-氧代己二酰-CoA溶液

0.5mM NADH

1mM二硫苏糖醇

10μm 3-氧代己二酰-CoA还原酶

接着，示出了对反应A、C～G进行催化的酶的具体例子。对生成3-氧代己二酰-CoA的反应A进行催化的酶例如可以使用酰基转移酶(β-酮硫解酶)等。作为酰基转移酶，对于基于EC编号的分类上没有特别地限定，但是优选归类于EC2.3.1.-的酰基转移酶，具体而言，可以列举出：作为3-氧代己二酰-CoA硫解酶的归类于EC2.3.1.174的酶、作为乙酰CoA C-乙酰基转移酶的归类于EC2.3.1.9的酶、作为乙酰CoA C-酰基转移酶的归类于EC2.3.1.16的酶。这些当中，优选使用来自Escherichia coli MG1655株的PaaJ(NCBI-Protein ID：NP415915)、来自Pseudomonas putida KT2440株的PcaF(NCBI-Protein ID：NP 743536)等。

上述酰基转移酶是否可以以琥珀酰-CoA和乙酰-CoA作为基质来生成3-氧代己二酰-CoA是通过以下方法确认的：组合通过精制酰基转移酶生成3-氧代己二酰-CoA的反应、以及以3-氧代己二酰-CoA作为基质的通过精制3-氧代己二酰-CoA还原酶的还原反应，测定伴随着3-氧代己二酰-CoA的还原的NADH减少量。具体的测定方法例如如下所述。

酰基转移酶活性的确认：按照常规方法，以作为对象的微生物株的基因组DNA为模板进行PCR，对对酰基转移酶进行编码的核酸的全长进行扩增。将该扩增片段插入到大肠菌用表达载体pACYCDuet-1(Novagen公司制造)的SacI位点上，使其成为与组氨酸标签序列相同的框架。将该质粒导入大肠菌BL 21(DE3)，按照常规方法，使用异丙基-β-硫代吡喃半乳糖苷(IPTG)诱导该酶的表达，接着利用来自培养液的组氨酸标签进行精制，得到酰基转移酶溶液。酰基转移酶活性可以通过这样来确认：使用该酶溶液制备以下组成的酶反应溶液，并测定伴随着30℃下的NADH的氧化的340nm吸收的减少。

100mM Tris-HCl(pH8.0)

10mM MgCl₂

0.1mM琥珀酰-CoA

0.2mM乙酰-CoA

0.2mM NADH

1mM二硫苏糖醇

10μg/mL 3-氧代己二酰-CoA还原酶

5μg/mL酰基转移酶

微生物所具有的酶是否具有酰基转移酶活性是通过使用CFE替代精制酰基转移酶来进行上述的测定而确认的。以大肠菌作为对象的具体测定方法例如如下所述。

CFE的制备：将一白金环的作为活性测定对象的大肠菌MG1655株接种在5mL的调整为pH7的培养基(培养基组成：10g/L的胰蛋白胨、5g/L的酵母提取物、5g/L的氯化钠)中，在30℃下振动培养18小时。将所得的培养液添加到5mL的调整为pH7的培养基(培养基组成：10g/L的胰蛋白胨、5g/L的酵母提取物、5g/L的氯化钠、2.5mM的阿魏酸、2.5mM的p-香豆酸、2.5mM的苯甲酸、2.5mM的cis,cis-己二烯二酸、2.5mM的原儿茶酸、2.5mM的儿茶酚、2.5mM的3OA、2.5mM的3-羟基己二酸、2.5mM的α-氢化己二烯二酸、2.5mM的己二酸、2.5mM的苯基乙胺)中，在30℃下振动培养3小时。

在所得的培养液中加入10mL的0.9％氯化钠，对菌体进行离心分离并除去上清液，重复操作3次以清洗菌体。将洗净后的菌体在由100mM的Tris-HCl(pH8.0)、1mM的二硫苏糖醇构成的1mL的Tris-HCl缓冲液中悬浊，并在所得的悬浊液中加入玻璃微珠(φ0.1mm)，使用超声波破碎机在4℃下破碎菌体。对菌体破碎液进行离心，并将所得的上清液当中的0.5mL经过UF膜(Amicon Ultra-0.5mL 10K、Merck Millipore公司制造)，除去了透过液后添加0.4mL的Tris-HCl缓冲液，重复进行3次以除去低分子量的杂质，然后再次在Tris-HCl缓冲液中悬浊成为液量为0.1mL的溶液，将其作为CFE。将替代精制酶的该CFE以相对于总量为0.1mL的酶反应溶液添加0.05mL，并且进行酶活性的确认。

对生成2,3-脱氢己二酰-CoA的反应C进行催化的酶例如可以使用烯酰-CoA水合酶等。作为烯酰-CoA水合酶，对于基于EC编号的分类上没有特别地限定，但是优选归类于EC编号4.2.1.-的烯酰-CoA水合酶，具体而言，可以列举出作为烯酰-CoA水合酶或2,3-脱氢己二酰-CoA水合酶的归类于EC4.2.1.17的酶。这些当中，优选使用来自Escherichia coliMG1655株的PaaF(NCBI-Protein ID：NP 415911)、来自Pseudomonas putida KT2440株的PaaF(NCBI-Protein ID：NP 745427)等。

由于烯酰-CoA水合酶所催化的反应一般为可逆反应，因此烯酰-CoA水合酶具有对以3-羟基己二酰-CoA作为基质生成2,3-脱氢己二酰-CoA的反应进行催化的活性是通过以下方法来确认的：以由α-氢化己二烯二酸通过酶反应所制备的2,3-脱氢己二酰-CoA作为基质，检测使用精制烯酰-CoA水合酶所生成3-羟基己二酰-CoA。具体的测定方法例如如下所述。

α-氢化己二烯二酸的制备：α-氢化己二烯二酸可以通过WO2016/199858A1的参考例1中所述的方法来制备。

2,3-脱氢己二酰-CoA溶液的制备：按照常规方法，以Pseudomonas putida KT2440株的基因组DNA为模板进行PCR，对对CoA转移酶进行编码的核酸(pcaI和pcaJ、NCBI-GeneID：1046613和1046612)的全长进行扩增。将该扩增片段插入到作为大肠菌用表达载体的pRSF-1b(Novagen公司制造)的KpnI位点上，使其成为与组氨酸标签序列相同的框架。将该质粒导入大肠菌BL 21(DE3)，按照常规方法，使用异丙基-β-硫代吡喃半乳糖苷(IPTG)诱导该酶的表达，接着利用来自培养液的组氨酸标签进行精制，得到CoA转移酶溶液。使用该溶液制备以下组成的2,3-脱氢己二酰-CoA制备用酶反应溶液，在30℃反应10分钟后，利用UF膜(Amicon Ultra-0.5mL10K、Merck Millipore公司制造)进行处理以除去酶，并将所得的透过液作为2,3-脱氢己二酰-CoA溶液。

2,3-脱氢己二酰-CoA制备用酶反应溶液

100mM Tris-HCl(pH8.0)

10mM MgCl₂

0.4mM琥珀酰-CoA

2mMα-氢化己二烯二酸钠盐

20μg/mL CoA转移酶

烯酰-CoA水合酶活性的确认：按照常规方法，以作为对象的微生物株的基因组DNA为模板进行PCR，对对烯酰-CoA水合酶进行编码的核酸的全长进行扩增。将该扩增片段插入到大肠菌用表达载体pET-16b(Novagen公司制造)的NdeI位点上，使其成为与组氨酸标签序列相同的框架。将该质粒导入大肠菌BL 21(DE3)，按照常规方法，使用异丙基-β-硫代吡喃半乳糖苷(IPTG)诱导该酶的表达，接着利用来自培养液的组氨酸标签进行精制，得到烯酰-CoA水合酶溶液。烯酰-CoA水合酶活性是通过以下方法来确认的：使用该溶液制备以下组成的酶反应溶液，在30℃反应10分钟后，利用UF膜(Amicon Ultra-0.5mL 10K、MerckMillipore公司制造)进行处理以除去酶，并且通过高效液相色谱-二级质谱联用仪(LC-MS/MS)(Agilent公司)检测所得的透过液中的3-羟基己二酰-CoA。

100mM Tris-HCl(pH8.0)

10mM MgCl₂

300μL/mL 2,3-脱氢己二酰-CoA溶液

1mM二硫苏糖醇

20μg/mL烯酰-CoA水合酶

本发明所使用的宿主微生物中本身表达的酶是否具有烯酰-CoA水合酶活性是通过将替代精制烯酰-CoA水合酶的CFE以相对于总量为0.1mL的酶反应溶液添加0.05mL，并且进行上述测定来确认的。以大肠菌作为对象的CFE的制备方法的具体例子如酰基转移酶活性的确认方法中所述。

对生成己二酰-CoA的反应D进行催化的酶例如可以使用烯酰-CoA还原酶等。作为烯酰-CoA还原酶，对于基于EC编号的分类上没有特别地限定，但是优选归类于EC编号1.3.-.-的烯酰-CoA还原酶，具体而言，可以列举出作为trans-2-烯酰-CoA还原酶的归类于EC1.3.1.44的酶、作为酰基-CoA脱氢酶的归类于EC1.3.8.7的酶。这些的具体例子例如在日本特表2011-515111、J Appl Microbiol.2015Oct；119(4):1057-63.等中公开，这些当中，优选使用来自Euglena gracilis Z株的TER(UniProt KB:Q5EU90)、来自Thermobifidafusca YX株的Tfu 1647(NCBI-Protein ID：AAZ55682)、来自Acinetobacter baylyi ADP1株的DcaA(NCBI-Protein ID：AAL09094.1)等。

烯酰-CoA还原酶具有以2,3-脱氢己二酰-CoA作为基质生成己二酰-CoA的活性是通过以下方法来确认的：以由α-氢化己二烯二酸通过酶反应所制备的2,3-脱氢己二酰-CoA作为基质，使用精制烯酰-CoA还原酶测定伴随着2,3-脱氢己二酰-CoA的还原的NADH减少量。

α-氢化己二烯二酸的制备、2,3-脱氢己二酰-CoA溶液的制备可以与上述同样地进行。

烯酰-CoA还原酶活性的确认：按照常规方法，以作为对象的微生物株的基因组DNA为模板进行PCR，对对烯酰-CoA还原酶进行编码的核酸的全长进行扩增。将该扩增片段插入到大肠菌用表达载体pET-16b(Novagen公司制造)的NdeI位点上，使其成为与组氨酸标签序列相同的框架。将该质粒导入大肠菌BL 21(DE3)，按照常规方法，使用异丙基-β-硫代吡喃半乳糖苷(IPTG)诱导该酶的表达，接着利用来自培养液的组氨酸标签进行精制，得到烯酰-CoA还原酶溶液。烯酰-CoA还原酶活性可以通过以下方法来确认：使用该酶溶液制备以下组成的酶反应溶液，测定伴随着30℃的NADH的氧化的340nm吸收的减少。

100mM Tris-HCl(pH8.0)

10mM MgCl₂

300μL/mL 2,3-脱氢己二酰-CoA溶液0.2mM NADH

1mM二硫苏糖醇

20μg/mL烯酰-CoA还原酶

本发明所使用的宿主微生物中本身表达的酶是否具有烯酰-CoA还原酶活性可以通过将替代精制烯酰-CoA还原酶的CFE以相对于总量为0.1mL的酶反应溶液添加0.05mL，并且进行上述测定来确认。以大肠菌作为对象的CFE的制备方法的具体例子如酰基转移酶活性的确认方法中所述。

对生成3-羟基己二酸的反应E、生成α-氢化己二烯二酸的反应F、生成己二酸的反应G进行催化的酶例如可以使用CoA转移酶或者酰基-CoA水合酶等，但是优选CoA转移酶。

作为CoA转移酶，对于基于EC编号的分类上没有特别地限定，但是优选归类于EC编号2.8.3.-的CoA转移酶，具体而言，可以列举出作为CoA转移酶或酰基-CoA转移酶的归类于EC2.8.3.6的酶等。

在本发明中，“CoA转移酶”是指具有以酰基-CoA和琥珀酸为基质生成羧酸和琥珀酰-CoA反应的催化活性(CoA转移酶活性)的酶。

在对生成3-羟基己二酸的反应E、生成α-氢化己二烯二酸的反应F进行催化的酶中，其中优选使用来自Pseudomonas putida KT2440株的PcaI和PcaJ(NCBI-Protein ID：NP746081和NP 746082)等。

另外，在对生成己二酸的反应G进行催化的酶中，优选使用来自Acinetobacterbaylyi ADP1株的DcaI和DcaJ(NCBI-Protein ID：CAG68538和CAG68539)等。

由于该酶反应为可逆反应，因此以3-羟基己二酰-CoA、2,3-脱氢己二酰-CoA或己二酰-CoA作为基质的CoA转移酶活性可以通过以下方法来确认：以3-羟基己二酸和琥珀酰-CoA、α-氢化己二烯二酸和琥珀酰-CoA、或者己二酸和琥珀酰-CoA作为基质，检测使用精制CoA转移酶所生成的3-羟基己二酰-CoA、2,3-脱氢己二酰-CoA或己二酰-CoA。具体的测定方法例如如下所述。

3-羟基己二酸的制备：3-羟基己二酸可以通过WO2016/199856A1的参考例1中所记载的方法来制备。

以3-羟基己二酸作为基质的CoA转移酶活性的确认：按照常规方法，以作为对象的微生物株的基因组DNA为模板进行PCR，对对CoA转移酶进行编码的核酸的全长进行扩增。将该扩增片段插入到大肠菌用表达载体pRSF-1b(Novagen公司制造)的KpnI位点上，使其成为与组氨酸标签序列相同的框架。将该质粒导入大肠菌BL 21(DE3)，按照常规方法，使用异丙基-β-硫代吡喃半乳糖苷(IPTG)诱导该酶的表达，接着利用来自培养液的组氨酸标签进行精制，得到CoA转移酶溶液。CoA转移酶活性是通过以下方法来确认的：使用该溶液制备以下组成的酶反应溶液，在30℃反应10分钟后，利用UF膜(Amicon Ultra-0.5mL 10K、MerckMillipore公司制造)进行处理以除去酶，并且通过高效液相色谱-二级质谱联用仪(LC-MS/MS)(Agilent公司)检测透过液中的3-羟基己二酰-CoA。

100mM Tris-HCl(pH8.0)

10mM MgCl₂

0.4mM琥珀酰-CoA

2mM 3-羟基己二酸钠盐

20μg/mL CoA转移酶

α-氢化己二烯二酸的制备：α-氢化己二烯二酸可以通过WO2016/199858A1的参考例1中所记载的方法来制备。

以α-氢化己二烯二酸作为基质的CoA转移酶活性的确认：按照常规方法，以作为对象的微生物株的基因组DNA为模板进行PCR，对对CoA转移酶进行编码的核酸的全长进行扩增。将该扩增片段插入到大肠菌用表达载体pRSF-1b(Novagen公司制造)的KpnI位点上，使其成为与组氨酸标签序列相同的框架。将该质粒导入大肠菌BL 21(DE3)，按照常规方法，使用异丙基-β-硫代吡喃半乳糖苷(IPTG)诱导该酶的表达，接着利用来自培养液的组氨酸标签进行精制，得到CoA转移酶溶液。CoA转移酶活性是通过以下方法来确认的：使用该溶液制备以下组成的酶反应溶液，在30℃反应10分钟后，利用UF膜(Amicon Ultra-0.5mL 10K、Merck Millipore公司制造)进行处理以除去酶，并且通过高效液相色谱-二级质谱联用仪(LC-MS/MS)(Agilent公司)检测透过液中的2,3-脱氢己二酰-CoA。

100mM Tris-HCl(pH8.0)

10mM MgCl₂

0.4mM琥珀酰-CoA

2mMα-氢化己二烯二酸钠盐

20μg/mL CoA转移酶

以己二酸作为基质的CoA转移酶活性的确认：按照常规方法，以作为对象的微生物株的基因组DNA为模板进行PCR，对对CoA转移酶进行编码的核酸的全长进行扩增。将该扩增片段插入到大肠菌用表达载体pRSF-1b(Novagen公司制造)的KpnI位点上，使其成为与组氨酸标签序列相同的框架。将该质粒导入大肠菌BL 21(DE3)，按照常规方法，使用异丙基-β-硫代吡喃半乳糖苷(IPTG)诱导该酶的表达，接着利用来自培养液的组氨酸标签进行精制，得到CoA转移酶溶液。CoA转移酶活性是通过以下方法来确认的：使用该溶液制备以下组成的酶反应溶液，在30℃反应10分钟后，利用UF膜(Amicon Ultra-0.5mL 10K、MerckMillipore公司制造)进行处理以除去酶，并且通过高效液相色谱-二级质谱联用仪(LC-MS/MS)(Agilent公司)检测透过液中的己二酰-CoA。

100mM Tris-HCl(pH8.0)

10mM MgCl₂

0.4mM琥珀酰-CoA

2mM己二酸钠盐

20μg/mL CoA转移酶

本发明所使用的宿主微生物中本身表达的酶是否具有CoA转移酶活性是通过将替代精制CoA转移酶的CFE以相对于总量为0.1mL的酶反应溶液添加0.05mL，并且进行上述测定来确认的。以大肠菌作为对象的CFE的制备方法的具体例子如酰基转移酶活性的确认方法中所述。

本发明中在将对PEP羧化激酶、酰基转移酶、3-氧代己二酰-CoA还原酶、烯酰-CoA水合酶、烯酰-CoA还原酶、CoA转移酶中的任意一种进行编码的核酸导入宿主微生物的情况下，该核酸可以基于存在于数据库中的该酶的氨基酸序列进行人工合成，也可以从天然中分离。在人工合成的情况下，可以根据导入的宿主微生物来改变对应于各氨基酸的密码子的使用频率。

在从天然中分离对该酶进行编码的核酸的情况下，对作为基因源的生物没有特别地限定，例如可以列举出：Acinetobacter baylyi、Acinetobacter radioresistens等不动杆菌属；Aerobacter cloacae等气杆菌属；Alcaligenes faecalis等产碱菌属；Bacillusbadius、Bacillus magaterium、Bacillus roseus等芽孢杆菌属；Brevibacterium iodinum等短杆菌属；Corynebacterium acetoacidophilum、Corynebacterium acetoglutamicum、Corynebacterium ammoniagenes、Corynebacterium glutamicum等棒状杆菌属；Cupriavidus metallidurans、Cupriavidus necator、Cupriavidus numazuensis、Cupriavidus oxalaticus等贪铜菌属；Delftia acidovorans等戴尔福特菌属；Escherichia coli、Escherichia fergusonii等大肠杆菌属；Hafnia alvei等哈夫尼菌属；Microbacterium ammoniaphilum等微杆菌属；Nocardioides albus等类诺卡氏菌属；Planomicrobium okeanokoites等游动微菌属；Pseudomonas azotoformans、Pseudomonaschlororaphis、Pseudomonas fluorescens、Pseudomonas fragi、Pseudomonas putida、Pseudomonas reptilivora等假单胞菌属；Rhizobium radiobacter等根瘤菌属；Rhodosporidium toruloides等红冬孢酵母菌属；Saccharomyces cerevisiae等酵母菌属；Serratia entomophila、Serratia ficaria、Serratia fonticola、Serratia grimesii、Serratia nematodiphila、Serratia odorifera、Serratia plymuthica等沙雷氏菌属；Shimwellia blattae等Shimwellia属；Streptomyces vinaceus、Streptomyceskarnatakensis、Streptomyces olivaceus、Streptomyces vinaceus等链霉菌属；Yarrowialipolytica等解脂耶氏酵母菌属；Yersinia ruckeri等耶尔森氏菌属；Euglena gracilis等眼虫属；Thermobifida fusca等嗜热菌属，但是优选不动杆菌属、棒状杆菌属、大肠杆菌属、假单胞菌属、沙雷氏菌属、眼虫属、嗜热菌属。

在本发明中，使丙酮酸激酶和磷酸转移酶类酶的功能受损是指使这些酶的活性受损。对于使功能受损的方法没有特别地限定，例如可以通过以下方法来使其受损：通过基因变异剂、紫外线照射等对编码该酶的基因进行基因变异处理；通过部位特异性变异法等对碱基序列的一部分或全部进行受损处理；向碱基序列导入移码突变；向碱基序列插入终止密码子等。另外，也可以通过使用基因重组技术除去碱基序列的全部或一部分、或者置换成其他碱基序列来使基因受损。这些当中，优选的是使碱基序列的一部分或全部受损的方法。

丙酮酸激酶归类于EC2.7.1.40，是一种可以对将磷酸烯醇丙酮酸脱磷酸化、并转化成丙酮酸和ATP的反应进行催化的酶。具体而言，可以列举出来自Escherichia colistr.K-12substr.MG1655株的pykF(NCBI-Protein ID：NP 416191、序列号178)、pykA(NCBI-Protein ID：NP 416368、序列号179)、以及来自Serratia grimesii NBRC13537株的pykF(序列号180)、pykA(序列号181)等。如下述路线2的代谢路径所示，在本发明中所使用的微生物具有2个以上的对丙酮酸激酶进行编码的基因的情况下，优选使全部丙酮酸激酶的功能受损。由本发明中所使用的微生物具有的基因编码的多肽是否为丙酮酸激酶可以通过在NCBI(National Center for Biotechnology Information)和KEGG(Kyoto Encyclopediaof Genes and Genomes)等网站上进行BLAST(Basic Local Alignment Search Tool)检索即可。

磷酸烯醇丙酮酸羧化激酶归类于EC4.1.1.49，是一种可以对从磷酸烯醇丙酮酸、二氧化碳以及ADP生成草酰乙酸和ATP的反应进行催化的酶。具体而言，可以列举出来自Escherichia coli str.K-12 substr.MG1655株的pck(NCBI-Protein ID：NP 417862、序列号182)、以及来自Serratia grimesii NBRC13537株的pckA 1(序列号183)、pckA 2(序列号184)等。

PEP羧化激酶在生理上承担着糖原异生过程中由脂肪酸生产葡萄糖时的主要反应。PEP羧化激酶所催化的反应是可逆反应，但是在糖原异生过程中该反应向将草酰乙酸转化为PEP和二氧化碳的方向进行。

由本发明中所使用的基因所编码的多肽是否为PEP羧化激酶可以通过在NCBI(National Center for Biotechnology Information)和KEGG(Kyoto Encyclopedia ofGenes and Genomes)等的网站上进行BLAST(Basic Local Alignment Search Tool)检索即可。

日本特开2015-146810号公报中记载了：使用代谢网络模型(network model)，利用In silico制作能够从乙酰-CoA和琥珀酰-CoA以高产率生产己二酸的微生物株时，PEP羧化激酶基因的受损是有效的。另外，日本特表2015-504688号公报中记载了：在经由PEP而生物合成己二烯二酸的制造中，以增加PEP库(pool)为目的，强化了PEP羧化激酶的活性。即，记载了PEP羧化激酶所催化的反应是向从草酰乙酸生成PEP的方向进行，根据该记载，本领域技术人员可以认为：由于PEP羧化激酶基因的表达增强导致了该酶活性的强化，预想会降低3-羟基己二酸、α-氢化己二烯二酸和/或己二酸的产率。然而，在本发明中发现，与上述的预想相反，当对具有制造3-羟基己二酸、α-氢化己二烯二酸和/或己二酸的能力、并且使丙酮酸激酶受损、增强了磷酸烯醇丙酮酸羧化激酶基因和对将3-氧代己二酰-CoA还原生成3-羟基己二酰-CoA的反应进行催化的酶基因的表达的基因修饰微生物进行培养时，3-羟基己二酸、α-氢化己二烯二酸和/或己二酸的产量提高。

本发明的基因修饰微生物进一步优选使磷酸转移酶类酶的功能受损。磷酸转移酶类酶是指phosphoenolpyruvate-dependent phosphotransferase system(PTS)(磷酸烯醇丙酮酸依赖性磷酸转移酶系统)(本说明书中有时记为PTS酶)。如下述路线2的代谢路径所示，PTS是用于将己糖、己糖醇、二糖等碳水化合物摄取至细胞内的主要机制。在PTS中，碳水化合物在被摄取至细胞的同时被转换为磷酸酯，另一方面，作为磷酸供给体的PEP转化为丙酮酸。

作为3-羟基己二酸、α-氢化己二烯二酸和/或己二酸的生物合成中的中间体的乙酰-CoA可以利用PTS酶的功能从PEP经由丙酮酸来合成。

[化学式2]

PTS酶如下构成：无论碳水化合物的种类如何都发挥作用的磷酸烯醇丙酮酸糖类磷酸转移酶I(phosphoenolpyruvate sugar phosphotransferase enzyme I)和磷酸载体蛋白HPr(phospho carrier protein HPr)这两种共通酶；以及对特定的碳水化合物特异性的膜结合糖类特异性渗透酶(membrane-bound sugar specific permeases)(酶II)。酶II进一步由sugar-specific IIA、IIB以及IIC成分构成。酶II的酶根据生物种类的不同以单个的或者多个连接成为蛋白结构域的状态存在。

在微生物中，磷酸烯醇丙酮酸糖类磷酸转移酶I被ptsI基因编码，磷酸载体蛋白HPr被ptsH基因编码，葡萄糖特异性IIA被crr基因编码，同样为葡萄糖特异性IIB和IIC则均被ptsG基因编码。

ptsG基因所编码的酶归类于EC2.7.1.199，称为蛋白-Npi-磷酸组氨酸-D-葡萄糖磷酸转移酶(protein-Npi-phosphohistidine-D-glucose phosphotransferase)，可以列举出来自Escherichia coli str.K-12substr.MG1655株的PtsG(NCBI-Gene ID：415619)、来自Serratia grimesii NBRC13537株的PtsG(序列号185)等。由本发明中所使用的微生物具有的基因编码的多肽是否为PTS酶可以通过在NCBI和KEGG等网站上进行BLAST检索即可。

在本发明中，可以使上述PTS酶基因当中的1种受损，也可以使其多种受损。可以使上述PTS酶基因中的任意一种受损，但优选使参与葡萄糖的摄取的酶基因受损，特别优选使ptsG基因受损。ptsG基因的具体例子可以列举出来自Escherichia coli str.K-12substr.MG1655株的ptsG(NCBI-Gene ID：945651)和来自Serratia grimesii NBRC13537株的ptsG(序列号243)等。

如上所述，大肠菌是具有制造3-羟基己二酸和α-氢化己二烯二酸的能力的微生物，日本特表2008-527991号公报中记载了：制作使对大肠菌的丙酮酸激酶进行编码的pykF和pykA以及对磷酸转移酶类酶进行编码的ptsG基因受损而得的变异株，并且当在厌氧条件下培养该变异株时，琥珀酸的产率增加，乙酸和乙醇的产率减少。这里，如上述路线2的代谢路径所示，乙酸和乙醇是由乙酰-CoA代谢所得的化合物。即，在日本特表2008-527991号公报中，通过使大肠菌的ptsG、pykF、pykA基因受损，可以推测乙酰-CoA的供给量降低，乙酸和乙醇的产率降低。

另一方面，如上所述，在本发明的方法中所制造的3-羟基己二酸、α-氢化己二烯二酸和/或己二酸是从由乙酰-CoA和琥珀酰-CoA通过反应A所生成的3-氧代己二酰-CoA经由多个反应代谢所得的化合物。因此，根据日本特表2008-527991号公报的记载，当使丙酮酸激酶和磷酸转移酶类酶的基因受损时，由于乙酰-CoA的供给量降低，因此可以预想3-羟基己二酸、α-氢化己二烯二酸和/或己二酸的产率也会降低。但是，在本发明中，与上述的预想相反，在强化了磷酸烯醇丙酮酸羧化激酶的活性和对将3-氧代己二酰-CoA还原生成3-羟基己二酰-CoA的反应进行催化的酶的活性的基因修饰微生物中，通过使丙酮酸激酶和磷酸转移酶类酶的基因受损，3-羟基己二酸、α-氢化己二烯二酸和/或己二酸的产率提高，并且乙酸和乙醇的产率也提高。

本发明的基因修饰微生物可以在含有以通常微生物可利用的碳源作为发酵原料的培养基中进行培养，优选在液体培养基中进行培养。也可以使用除了该基因修饰微生物可利用的碳源以外，还进一步含有氮源、无机盐、以及根据需要的氨基酸或维生素等有机微量营养素的培养基。只要含有上述营养源，则无论是天然培养基还是合成培养基均可以利用。

发酵原料是指该基因修饰微生物可以代谢的原料。“代谢”是指微生物从细胞外吸收的、或者细胞内由其他化学物质生成的某种化学物质通过酶反应而转化为其他化学物质。作为碳源，优选使用糖类。作为糖类的具体例子，可以列举出：葡萄糖、蔗糖、果糖、半乳糖、甘露糖、木糖、阿拉伯糖等单糖类；这些单糖类结合而成的二糖类；多糖类；以及含有它们的淀粉糖化液、糖蜜、含纤维素生物质糖化液等。

上述列举的碳源可以只使用一种，也可以组合使用，但是特别优选在含有葡萄糖的培养基中进行培养。在碳源的添加中，对培养基中的碳源的浓度没有特别地限定，可以根据碳源的种类等进行适当设定。葡萄糖的优选的浓度为5g/L～300g/L。

作为用于培养该基因修饰微生物的氮源，例如可以使用氨气、氨水、铵盐类、尿素、硝酸盐类、其他辅助使用的有机氮源：例如油渣类、大豆水解液、酪蛋白分解物、其他氨基酸、维生素类、玉米浆、酵母或酵母提取物、肉精、胰蛋白胨等肽类、各种发酵菌体及其水解物等。对培养基中氮源的浓度没有特别地限定，优选为0.1g/L～50g/L。

作为用于培养该基因修饰微生物的无机盐类，例如可以适当添加使用磷酸盐、镁盐、钙盐、铁盐以及锰盐等。

用于生产3-羟基己二酸、α-氢化己二烯二酸和/或己二酸的基因修饰微生物的培养条件，可以根据该基因修饰微生物的种类和外部条件等，对上述成分组成的培养基、培养温度、搅拌速度、pH、通气量、接种量等进行适当地调节或选择设定。

培养中的pH的范围只要能够使该基因修饰微生物生长即可，没有特别地限定，优选为pH5～8、更优选为pH5.5～6.8。

培养中的通气条件的范围只要能够生产3-羟基己二酸、α-氢化己二烯二酸和/或己二酸即可，没有特别地限定，但是为了良好地生长该微生物变异体，优选至少在培养开始时在培养容器内的气相和/或液相中残留有氧气。

在液体培养中有发泡的情况下，可以将矿物油、硅油以及表面活性剂等消泡剂适当地混合到培养基中。

在所述微生物的培养物中生成3-羟基己二酸、α-氢化己二烯二酸和/或己二酸直到可回收的量后，可以将所生产的该产物回收。对于所生产的该产物的回收，例如分离可以在累积量适度提高的时候停止培养，并按照从该培养物中提取发酵产物的一般方法来进行。具体而言，通过离心分离、过滤等将菌体分离后，通过柱层析色谱、离子交换层析色谱、活性炭处理、结晶、膜分离、蒸馏等将该产物从培养物中分离出来。更具体而言，可以列举出：通过在该产物的盐中添加酸成分来回收析出物的方法；利用反渗透膜或旋蒸仪等对培养物进行浓缩操作以除去水，提高该产物的浓度后，通过冷却结晶或绝热结晶使该产物和/或该产物的盐的结晶析出，再通过离心分离或过滤等获得该产物和/或该产物的盐的结晶的方法；在培养物中添加醇类以使将该产物成为酯后，通过蒸馏操作回收该产物的酯，然后再通过水解获得该产物的方法等，但不限于此。另外，这些回收方法可以根据产物的物性等适当地选择并优化。

实施例

以下，列举实施例并对本发明进行具体的说明。

参考例1

用于表达对生成3-氧代己二酰-CoA和辅酶A的反应(反应A)进行催化的酶、对从3-羟基己二酰-CoA生成3-羟基己二酸的反应(反应E)以及对从2,3-脱氢己二酰-CoA生成α-氢化己二烯二酸的反应(反应F)进行催化的酶、以及序列号1、2、3、4、5、6、7所述的多肽的质粒的制作

使用XhoI切断大肠菌内可自体复制的载体pBBR1MCS-2(ME Kovach,(1995),Gene166:175-176)，得到pBBR1MCS-2/XhoI。为了将构成性表达启动子组装在该载体中，以Escherichia coli K-12MG1655的基因组DNA为模板，设计了用于对gapA(NCBI Gene ID：NC000913.3)的上游区域200b(序列号186)进行PCR扩增的引物(序列号187、188)，按照常规方法进行PCR反应。使用In-Fusion HD Cloning Kit(“タカラバイオ株式会社”制造)将所得的片段和pBBR1MCS-2/XhoI连接起来，并导入大肠菌株DH5α中。从所得的重组大肠菌株中提取该质粒，将按照常规方法确认了碱基序列的质粒设为pBBR1MCS-2::Pgap。接着，使用ScaI切断pBBR1MCS-2::Pgap，得到pBBR1MCS-2::Pgap/ScaI。为了对催化反应A的酶进行编码的基因进行扩增，以Pseudomonas putida KT2440株的基因组DNA为模板，设计了用于对酰基转移酶基因pcaF(NCBI Gene ID：1041755、序列号189)的全长进行PCR扩增的引物(序列号190、191)，按照常规方法进行PCR反应。使用In-Fusion HD Cloning Kit将所得的片段和pBBR1MCS-2::Pgap/ScaI连接起来，并导入大肠菌株DH5α中。从所得的重组株中提取该质粒，将按照常规方法确认了碱基序列的质粒设为pBBR1MCS-2::AT。接着，使用HpaI切断pBBR1MCS-2::AT，得到pBBR1MCS-2::AT/HpaI。为了对催化反应D和反应E的酶进行编码的基因进行扩增，以Pseudomonas putida KT2440株的基因组DNA为模板，设计了用于对包含CoA转移酶基因pcaI和pcaJ(NCBI Gene ID：1046613、1046612，序列号192、193)的全长的连续序列进行PCR扩增的引物(序列号194、195)，按照常规方法进行PCR反应。使用In-Fusion HDCloning Kit将所得的片段和pBBR1MCS-2::AT/HpaI连接起来，并导入大肠菌株DH5α中。从所得的重组株中提取该质粒，将按照常规方法确认了碱基序列的质粒设为pBBR1MCS-2::ATCT。

使用ScaI切断pBBR1MCS-2::ATCT，得到pBBR1MCS-2:ATCT/ScaI。为了对对序列号1的多肽进行编码的核酸进行扩增，以Serratia marcescens ATCC13880株的基因组DNA为模板，设计了用于对序列号87所述的核酸进行扩增的引物(序列号196、197)，按照常规方法进行PCR反应。为了对对序列号2的多肽进行编码的核酸进行扩增，以Serratianematodiphila DSM21420株的基因组DNA为模板，设计了用于对序列号88所述的核酸进行扩增的引物(序列号198、199)，按照常规方法进行PCR反应。为了对对序列号3的多肽进行编码的核酸进行扩增，以Serratia plymuthica NBRC102599株的基因组DNA为模板，设计了用于对序列号89所述的核酸进行扩增的引物(序列号200、201)，按照常规方法进行PCR反应。为了对对序列号4的多肽进行编码的核酸进行扩增，以Serratia proteamaculans 568株的基因组DNA为模板，设计了用于对序列号90所述的核酸进行扩增的引物(序列号202、203)，按照常规方法进行PCR反应。为了对对序列号5的多肽进行编码的核酸进行扩增，以Serratia ureilytica Lr5/4株的基因组DNA为模板，设计了用于对序列号91所述的核酸进行扩增的引物(序列号204、205)，按照常规方法进行PCR反应。为了对对序列号6的多肽进行编码的核酸进行扩增，以Serratia sp.BW106株的基因组DNA为模板，设计了用于对序列号92所述的核酸进行扩增的引物(序列号206、207)，按照常规方法进行PCR反应。为了对对序列号7的多肽进行编码的核酸进行扩增，以Serratia liquefaciens FK01株的基因组DNA为模板，设计了用于对序列号93所述的核酸进行扩增的引物(序列号208、209)，按照常规方法进行PCR反应。使用In-Fusion HD Cloning Kit(“タカラバイオ株式会社”制造)将分别得到的片段和pBBR1MCS-2::ATCT/ScaI连接起来，并导入大肠菌株DH5α中。从所得的重组株中提取该质粒，按照常规方法确认了碱基序列。

将用于表达序列号1所述的多肽的质粒设为“pBBR1MCS-2::ATCTOR1”；用于表达序列号2所述的多肽的质粒设为“pBBR1MCS-2::ATCTOR2”；用于表达序列号3所述的多肽的质粒设为“pBBR1MCS-2::ATCTOR3”；用于表达序列号4所述的多肽的质粒设为“pBBR1MCS-2::ATCTOR4”；用于表达序列号5所述的多肽的质粒设为“pBBR1MCS-2::ATCTOR5”；用于表达序列号6所述的多肽的质粒设为“pBBR1MCS-2::ATCTOR6”；用于表达序列号7所述的多肽的质粒设为“pBBR1MCS-2::ATCTOR7”，这些质粒如表4所示。

[表4]

参考例2

用于表达对从3-羟基己二酰-CoA生成2,3-脱氢己二酰-CoA的反应(反应C)进行催化的酶的质粒的制作

使用SacI切断大肠菌内可自体复制的表达载体pMW119(“ニッポンジーン”公司制造)，得到pMW119/SacI。为了将构成性表达启动子组装在该载体中，以Escherichia coliK-12MG1655的基因组DNA为模板，设计了用于对gapA(NCBI Gene ID：NC 000913.3)的上游区域200b(序列号186)进行PCR扩增的引物(序列号210、211)，按照常规方法进行PCR反应。使用In-Fusion HD Cloning Kit(“タカラバイオ株式会社”制造)将所得的片段和pMW119/SacI连接起来，并导入大肠菌株DH5α中。从所得的重组大肠菌株中提取该质粒，将按照常规方法确认了碱基序列的质粒设为pMW119::Pgap。接着，使用SphI切断pMW119::Pgap，得到pMW119::Pgap/SphI。为了对催化反应C的酶进行编码的基因进行扩增，以Pseudomonasputida KT2440株的基因组DNA为模板，设计了用于对烯酰-CoA水合酶基因paaF(NCBI GeneID：1046932、序列号176)的全长进行PCR扩增的引物(序列号212、213)，按照常规方法进行PCR反应。使用In-Fusion HD Cloning Kit(“タカラバイオ株式会社”制造)将所得的片段和pMW119::Pgap/SphI连接起来，并导入大肠菌株DH5α中。从所得的重组株中提取该质粒，按照常规方法确认了碱基序列。将所得的质粒设为“pMW119::EH”。

参考例3

用于表达对从乙酰-CoA和琥珀酰-CoA生成3-氧代己二酰-CoA和辅酶A的反应(反应A)进行催化的酶、对从己二酰-CoA生成己二酸的反应(反应G)进行催化的酶、以及序列号1、2、3、4、5、6、7所述的多肽的质粒的制作

为了对对催化反应G的酶进行编码的基因进行扩增，以Acinetobacter baylyiADP1株的基因组DNA为模板，设计了用于对包含CoA转移酶基因dcaI和dcaJ(NCBI Gene ID：CR543861.1，序列号239、240)的全长的连续序列进行PCR扩增的引物(序列号241、242)，按照常规方法进行PCR反应。使用In-Fusion HD Cloning Kit将所得的片段以及使用HpaI分别将参考例1所制作的pBBR1MCS-2::ATCTOR1、pBBR1MCS-2::ATCTOR2、pBBR1MCS-2::ATCTOR3、pBBR1MCS-2::ATCTOR4、pBBR1MCS-2::ATCTOR5、pBBR1MCS-2::ATCTOR6、pBBR1MCS-2::ATCTOR7切断所得的片段连接起来，并导入大肠菌株DH5α中。从所得的重组株中提取该质粒，将按照常规方法确认了碱基序列的质粒分别设为pBBR1MCS-2::ATCT2OR1、pBBR1MCS-2::ATCT2OR2、pBBR1MCS-2::ATCT2OR3、pBBR1MCS-2::ATCT2OR4、pBBR1MCS-2::ATCT2OR5、pBBR1MCS-2::ATCT2OR6、pBBR1MCS-2::ATCT2OR7。

参考例4

用于表达对从3-羟基己二酰-CoA生成2,3-脱氢己二酰-CoA的反应(反应C)和从2,3-脱氢己二酰-CoA生成己二酰-CoA的反应(反应D)进行催化的酶的质粒的制作

使用HindIII切断pMW119::EH，得到pMW119::EH/HindIII。为了对对催化反应D的酶进行编码的基因进行扩增，设计了对来自Acinetobacter baylyi ADP1株的dcaA(NCBI-Protein ID：AAL09094.1，序列号214)的全长进行PCR扩增的引物(序列号215、216)，按照常规方法进行PCR反应。使用In-Fusion HD Cloning Kit(“タカラバイオ株式会社”制造)将所得的片段和pMW119::EH/HindIII连接起来，并导入大肠菌株DH5α中。从所得的重组株中提取该质粒，将按照常规方法确认了碱基序列的质粒设为pMW119::EHER。

参考例5

用于表达PEP羧化激酶、对生成3-氧代己二酰-CoA和辅酶A的反应(反应A)进行催化的酶、对从3-羟基己二酰-CoA生成3-羟基己二酸的反应(反应E)以及对从2,3-脱氢己二酰-CoA生成α-氢化己二烯二酸的反应(反应F)进行催化的酶、以及序列号1、2、3、4、5、6、7所述的多肽的质粒的制作

为了组装构成性表达PEP羧化激酶的启动子，以Escherichia coli K-12MG1655的基因组DNA为模板，设计了用于对gapA(NCBI Gene ID：NC 000913.3)的上游区域200b(序列号186)进行PCR扩增的引物(序列号217、218)，按照常规方法进行PCR反应。使用In-FusionHD Cloning Kit将所得的片段以及使用SacI分别将参考例1所制作的pBBR1MCS-2::ATCTOR1、pBBR1MCS-2::ATCTOR2、pBBR1MCS-2::ATCTOR3、pBBR1MCS-2::ATCTOR4、pBBR1MCS-2::ATCTOR5、pBBR1MCS-2::ATCTOR6、pBBR1MCS-2::ATCTOR7切断所得的片段连接起来，并导入大肠菌株DH5α中。从所得的重组株中提取该质粒，将按照常规方法确认了碱基序列的质粒分别设为pBBR1MCS-2::ATCTOR1Pgap、pBBR1MCS-2::ATCTOR2Pgap、pBBR1MCS-2::ATCTOR3Pgap、pBBR1MCS-2::ATCTOR4Pgap、pBBR1MCS-2::ATCTOR5Pgap、pBBR1MCS-2::ATCTOR6Pgap、pBBR1MCS-2::ATCTOR7Pgap。接着，为了对对PEP羧化激酶进行编码的基因进行扩增，以Serratia grimesii NBRC13537株的基因组DNA为模板，设计了用于对包含PEP羧化激酶基因(序列号219)的全长的连续序列进行PCR扩增的引物(序列号220、221)，按照常规方法进行PCR反应。使用In-Fusion HD Cloning Kit将所得的片段以及使用SacI分别将pBBR1MCS-2::ATCTOR1Pgap、pBBR1MCS-2::ATCTOR2Pgap、pBBR1MCS-2::ATCTOR3Pgap、pBBR1MCS-2::ATCTOR4Pgap、pBBR1MCS-2::ATCTOR5Pgap、pBBR1MCS-2::ATCTOR6Pgap、pBBR1MCS-2::ATCTOR7Pgap切断所得片段连接起来，并导入大肠菌株DH5α中。从所得的重组株中提取该质粒，将按照常规方法确认了碱基序列的质粒分别设为pBBR1MCS-2::ATCTOR1PCK、pBBR1MCS-2::ATCTOR2PCK、pBBR1MCS-2::ATCTOR3PCK、pBBR1MCS-2::ATCTOR4PCK、pBBR1MCS-2::ATCTOR5PCK、pBBR1MCS-2::ATCTOR6PCK、pBBR1MCS-2::ATCTOR7PCK。

参考例6

用于表达PEP羧化激酶、对从乙酰-CoA和琥珀酰-CoA生成3-氧代己二酰-CoA和辅酶A的反应(反应A)进行催化的酶、对从己二酰-CoA生成己二酸的反应(反应G)进行催化的酶、以及序列号1、2、3、4、5、6、7所述的多肽的质粒的制作

使用与参考例5同样的方法和质粒，将以Escherichia coli K-12MG1655的基因组DNA为模板的gapA(NCBI Gene ID：NC 000913.3)的上游区域200b和来自Serratiagrimesii NBRC13537株的PEP羧化激酶基因插入到参考例3中所制作的pBBR1MCS-2::ATCT2OR1、pBBR1MCS-2::ATCT2OR2、pBBR1MCS-2::ATCT2OR3、pBBR1MCS-2::ATCT2OR4、pBBR1MCS-2::ATCT2OR5、pBBR1MCS-2::ATCT2OR6、pBBR1MCS-2::ATCT2OR7中。将所得的质粒分别设为pBBR1MCS-2::ATCT2OR1PCK、pBBR1MCS-2::ATCT2OR2PCK、pBBR1MCS-2::ATCT2OR3PCK、pBBR1MCS-2::ATCT2OR4PCK、pBBR1MCS-2::ATCT2OR5PCK、pBBR1MCS-2::ATCT2OR6PCK、pBBR1MCS-2::ATCT2OR7PCK。

实施例1

使丙酮酸激酶的功能受损了的沙雷氏菌属微生物变异体的制作

使对沙雷氏菌属微生物的丙酮酸激酶进行编码的基因pykF和pykA受损，从而制作了使丙酮酸激酶的功能受损了的沙雷氏菌属微生物变异体。

pykF和pykA的受损方法可以根据Proc Natl Acad Sci USA.2000 Jun 6；97(12):6640-6645.中所记载的方法。

使pykF受损了的沙雷氏菌属微生物变异体的制作

以pKD4为模板，使用序列号222和223的寡聚DNA作为引物来进行PCR，得到用于使pykF受损的序列长度1.6kb的PCR片段。将作为FRT重组酶(FRT recombinase)表达质粒的pKD46导入Serratia grimesii NBRC13537株中，获得了氨苄西林耐性株。将所得的菌株接种在含有500μg/mL氨苄西林的5mL的LB培养基中，在30℃下振动培养1天。将0.5mL的培养液接种在含有500μg/mL氨苄西林和50mM阿拉伯糖的50mL的LB培养基中，在30℃下旋转培养2小时。将培养液冰冷20分钟后，使用10％(w/w)的丙三醇将菌体清洗3次。使用100μL的10％(w/w)的丙三醇将清洗后的团粒悬浊，与5μL的PCR片段混合后，在电穿孔液槽(Cuvette)内冰冷10分钟。使用Gene pulser(Bio-rad公司制造)实施电穿孔(3kV、200Ω、25μF)后，立即添加1mL的SOC培养基，在30℃下振动培养2小时。全部涂布在包含25μg/mL卡那霉素的LB琼脂培养基上，在30℃下进行孵育1天。使用所得的卡那霉素耐性株进行菌落直接PCR，根据条带长度确认了目的基因的受损和卡那霉素耐性基因的插入。引物使用了序列号224和226的寡聚DNA。

接着，将该卡那霉素耐性株接种在5mL的LB培养基中，通过在37℃下进行2次继代培养使pKD46脱落，得到氨苄西林敏感性株。在氨苄西林敏感性株中导入pCP20，再次获得氨苄西林耐性株。将所得的菌株在40℃下培养后进行菌落直接PCR，根据条带长度确认了卡那霉素耐性基因的脱落。引物使用了序列号225和226的寡聚DNA。将卡那霉素敏感性株接种在5mL的LB培养基中，通过在37℃下进行2次继代培养使pCP20脱落。将所得的菌株设为Serratia grimesii NBRC13537ΔpykF。

使pykA受损了的沙雷氏菌属微生物变异体的制作

以pKD4为模板，使用序列号227和228的寡聚DNA作为引物来进行PCR，得到序列长度1.6kb的pykA受损用PCR片段。

通过与pykF受损株的制作同样的方法，使Serratia grimesii NBRC13537ΔpykF株的pykA受损。在该株中导入pKD46后导入pykA受损用PCR片段。使用所得的卡那霉素耐性株进行菌落直接PCR，根据条带长度确认了目的基因的受损和卡那霉素耐性基因的插入。引物使用了序列号224和230的寡聚DNA。

接着，使pKD46脱落，得到氨苄西林敏感性株。在氨苄西林敏感性株中导入pCP20，再次获得氨苄西林耐性株。使用所得的菌株进行菌落直接PCR，根据条带长度确认了卡那霉素耐性基因的脱落。引物使用了序列号229和230的寡聚DNA。使pCP20从卡那霉素敏感性株脱落。将所得的菌株设为SgΔPP。

实施例2

导入有使丙酮酸激酶的功能受损、并且表达PEP羧化激酶和对反应A、B、E以及F进行催化的酶的质粒的沙雷氏菌属微生物变异体的制作

对于实施例1所制作的SgΔPP，分别导入参考例1所制作的质粒，制作了沙雷氏菌属微生物变异体。

将SgΔPP接种在5mL的LB培养基中，在30℃下振动培养1天。将0.5mL的培养液接种在5mL的LB培养基中，在30℃下振动培养2小时。将培养液冰冷20分钟后，使用10％(w/w)的丙三醇将菌体清洗3次。使用100μL的10％(w/w)的丙三醇将清洗后的团粒悬浊，与1μL的pBBR1MCS-2::ATCTOR1PCK、pBBR1MCS-2::ATCTOR2 PCK、pBBR1MCS-2::ATCTOR3PCK、pBBR1MCS-2::ATCTOR4PCK、pBBR1MCS-2::ATCTOR5PCK、pBBR1MCS-2::ATCTOR6PCK或pBBR1MCS-2::ATCTOR7PCK混合后，在电穿孔液槽内冰冷10分钟。使用Gene pulser(Bio-rad公司制造)实施电穿孔(3kV、200Ω、25μF)后，立即添加1mL的SOC培养基，在30℃下振动培养1小时。将50μL涂布在包含25μg/mL卡那霉素的LB琼脂培养基上，在30℃下进行孵育1天。将所得的菌株分别设为SgΔPP/3HA1PCK、SgΔPP/3HA2PCK、SgΔPP/3HA3PCK、SgΔPP/3HA4PCK、SgΔPP/3HA5PCK、SgΔPP/3HA6PCK、SgΔPP/3HA7PCK。

参考例7

导入有不使丙酮酸激酶的功能受损、并且表达PEP羧化激酶和对反应A、B、E以及F进行催化的酶的质粒的沙雷氏菌属微生物变异体的制作

通过与实施例2同样的方法，在Serratia grimesii NBRC 13537中导入pBBR1MCS-2::ATCTOR1PCK、pBBR1MCS-2::ATCTOR2PCK、pBBR1MCS-2::ATCTOR3PCK、pBBR1MCS-2::ATCTOR4PCK、pBBR1MCS-2::ATCTOR5PCK、pBBR1MCS-2::ATCTOR6PCK或pBBR1MCS-2::ATCTOR7PCK。另外，作为对照，制作了在Serratia grimesii NBRC 13537中导入有作为空白载体的pBBR1MCS-2的菌株。将所得的菌株分别设为Sg/3HA1PCK、Sg/3HA2PCK、Sg/3HA3PCK、Sg/3HA4PCK、Sg/3HA5PCK、Sg/3HA6PCK、Sg/3HA7PCK、Sg/pBBR(阴性对照)。

参考例8

导入有使丙酮酸激酶的功能受损、并且表达对反应A、B、E以及F进行催化的酶的质粒的沙雷氏菌属微生物变异体的制作

通过与实施例2同样的方法，在SgΔPP中导入pBBR1MCS-2::ATCTOR1、pBBR1MCS-2::ATCTOR2、pBBR1MCS-2::ATCTOR3、pBBR1MCS-2::ATCTOR4、pBBR1MCS-2::ATCTOR5、pBBR1MCS-2::ATCTOR6或pBBR1MCS-2::ATCTOR7。另外，作为对照，制作了在SgΔPP中导入有作为空白载体的pBBR1MCS-2的菌株。将所得的菌株分别设为SgΔPP/3HA1、SgΔPP/3HA2、SgΔPP/3HA3、SgΔPP/3HA4、SgΔPP/3HA5、SgΔPP/3HA6、SgΔPP/3HA7、SgΔPP/pBBR(阴性对照)。

实施例3

利用使丙酮酸激酶的功能受损了的、并且强化了PEP羧化激酶的活性的沙雷氏菌属微生物变异体生产3-羟基己二酸和α-氢化己二烯二酸的试验

利用实施例2中所制作的沙雷氏菌属微生物变异体，进行3-羟基己二酸和α-氢化己二烯二酸的生产试验。

将一白金环的实施例2中所制作的变异体接种在5mL的调整为pH7的培养基I(10g/L的细菌用胰蛋白胨(Difco Laboratories公司制造)、5g/L的细菌用酵母提取物(DifcoLaboratories公司制造)、5g/L的氯化钠、25μg/mL的卡那霉素)(φ18mm的玻璃试管、铝栓)中，在30℃、120min-1下振动培养24小时。将0.25mL该培养液添加到5mL的调整为pH6.5的培养基II(50g/L的葡萄糖、1g/L的硫酸铵、50mM的磷酸钾、0.025g/L的硫酸镁、0.0625mg/L的硫酸铁、2.7mg/L的硫酸锰、0.33mg/L的氯化钙、1.25g/L的氯化钠、2.5g/L的细菌用胰蛋白胨、1.25g/L的细菌用酵母提取物、25μg/mL的卡那霉素)(φ18mm的玻璃试管、铝栓)中，在30℃、120min-1下振动培养24小时。

基质和产物的定量分析

使用Millex-GV(0.22μm、PVDF、Merck公司制造)对来自该培养液的菌体离心分离后的上清液进行膜处理，通过以下方法对透过液进行分析，从而对培养上清液中积累的3-羟基己二酸、α-氢化己二烯二酸以及其他产物、培养基中未被利用而残存的糖类的浓度进行定量。进一步，根据该结果并使用下式(2)算出的3-羟基己二酸和α-氢化己二烯二酸的产率如表5所示。

但是，在利用LC-MS/MS进行的定量分析中，将0.1mg/L以下的情况设为检测限以下，在表中表示为N.D。

产率(％)＝产物的生成量(mol)/糖的消耗量(mol)×100···式(2)

利用LC-MS/MS进行的3-羟基己二酸和α-氢化己二烯二酸的定量分析

·HPLC：1290Infinity(Agilent Technologies公司制造)

柱子：Synergi hydro-RP(Phenomenex公司制造)、长度100mm、内径3mm、粒径2.5μm

流动相：0.1％甲酸水溶液/甲醇＝70/30

流速：0.3mL/分钟

柱温：40℃

LC检测器：1260DAD VL+(210nm)

·MS/MS：Triple-Quad LC/MS(Agilent Technologies公司制造)

电离法：ESI负模式。

利用HPLC进行的有机酸的定量分析

·HPLC：LC-10A(“島津製作所”制造)

柱子：Shim-pack SPR-H(“島津ジーエルシー”公司制造)、长度250mm、内径7.8mm、粒径8μm

Shim-pack SCR-101H(“島津ジーエルシー”公司制造)、长度250mm、内径7.8mm、粒径10μm

流动相：5mM p-甲苯磺酸

反应液：5mM p-甲苯磺酸、0.1mM EDTA、20mM Bis-Tris

流速：0.8mL/分钟

柱温：45℃

检测器：CDD-10Avp(“島津製作所”制造)

利用HPLC进行的糖类和醇类的定量分析

·HPLC：Shimazu Prominence(“島津製作所”制造)

柱子：Shodex Sugar SH1011(“昭和電工株式会社”制造)、长度300mm、内径8mm、粒径6μm

流动相：0.05M硫酸水溶液

流速：0.6mL/分钟

柱温：65℃

检测器：RID-10A(“島津製作所”制造)。

比较例1

利用未使丙酮酸激酶的功能受损、并且强化了PEP羧化激酶的活性的沙雷氏菌属微生物变异体生产3-羟基己二酸和α-氢化己二烯二酸的试验

通过与实施例3同样的方法，对参考例7所制作的变异体进行培养。对培养上清液中积累的3-羟基己二酸、α-氢化己二烯二酸以及其他产物、培养基中未被利用而残存的糖类的浓度进行定量。进一步，根据该结果并使用上式(2)算出的3-羟基己二酸和α-氢化己二烯二酸的产率如表5所示。

比较例2

利用使丙酮酸激酶的功能受损了、并且未强化PEP羧化激酶的活性的沙雷氏菌属微生物变异体生产3-羟基己二酸和α-氢化己二烯二酸的试验

通过与实施例3同样的方法，对参考例8所制作的变异体进行培养。对培养上清液中积累的3-羟基己二酸、α-氢化己二烯二酸以及其他产物、培养基中未被利用而残存的糖类的浓度进行定量。进一步，根据该结果并使用上式(2)算出的3-羟基己二酸和α-氢化己二烯二酸的产率如表5所示。

当将比较例1和2的结果与实施例3的结果进行比较时，可知：通过使沙雷氏菌属微生物的丙酮酸激酶的功能受损、并且强化PEP羧化激酶的活性，可以提高3-羟基己二酸和α-氢化己二烯二酸的产率。

[表5]

实施例4

使丙酮酸激酶的功能受损了的大肠菌变异体的制作

使对大肠菌的丙酮酸激酶进行编码的基因pykF和pykA受损，从而制作了使丙酮酸激酶的功能受损了的大肠菌变异体，pykF和pykA的受损方法可以根据Proc Natl Acad SciUSA.2000Jun 6；97(12):6640-6645.中所记载的方法。

使pykF受损了的大肠菌的变异体的制作

以pKD4为模板，使用序列号231和232的寡聚DNA作为引物进行PCR，得到用于pykF受损的序列长度1.6kb的PCR片段。

将作为FRT重组酶表达质粒的pKD46导入Escherichia coli MG1655株中，获得了氨苄西林耐性株。将所得的菌株接种在含有100μg/mL氨苄西林的5mL的LB培养基中，在30℃下振动培养1天。将0.5mL培养液接种在含有100μg/mL氨苄西林和50mM阿拉伯糖的50mL的LB培养基中，在30℃下旋转培养2小时。将培养液冰冷20分钟后，使用10％(w/w)的丙三醇将菌体清洗3次。使用100μL的10％(w/w)的丙三醇将清洗后的团粒悬浊，与5μL的PCR片段混合后，在电穿孔液槽内冰冷10分钟。使用Gene pulser(Bio-rad公司制造)实施电穿孔(3kV、200Ω、25μF)后，立即添加1mL的SOC培养基，在30℃下振动培养2小时。全部涂布在包含25μg/mL卡那霉素的LB琼脂培养基上，在30℃下进行孵育1天。使用所得的卡那霉素耐性株进行菌落直接PCR，根据条带长度确认了目的基因的受损和卡那霉素耐性基因的插入。引物使用了序列号224和234的寡聚DNA。

接着，将该卡那霉素耐性株接种在5mL的LB培养基中，通过在37℃下进行2次继代培养使pKD46脱落，得到氨苄西林敏感性株。在氨苄西林敏感性株中导入pCP20，再次获得氨苄西林耐性株。将所得的菌株在40℃下培养后进行菌落直接PCR，根据条带长度确认了卡那霉素耐性基因的脱落。引物使用了序列号233和234的寡聚DNA。将卡那霉素敏感性株接种在5mL的LB培养基中，通过在37℃下进行2次继代培养使pCP20脱落。将所得的菌株设为Escherichia coli MG1655ΔpykF。

使pykA受损了的大肠菌变异体的制作

以pKD4为模板，使用序列号235和236的寡聚DNA作为引物进行PCR，得到序列长度1.6kb的pykA受损用PCR片段。

通过与pykF受损株的制作同样的方法，使Escherichia coli MG1655ΔpykF株的pykA受损。在该菌株中导入pKD46后导入pykA受损用PCR片段。使用所得的卡那霉素耐性株进行菌落直接PCR，根据条带长度确认了目的基因的受损和卡那霉素耐性基因的插入。引物使用了序列号224和238的寡聚DNA。

接着，使pKD46脱落，得到氨苄西林敏感性株。在氨苄西林敏感性株中导入pCP20，再次获得氨苄西林耐性株。使用所得的菌株进行菌落直接PCR，根据条带长度确认了卡那霉素耐性基因的脱落。引物使用了序列号237和238的寡聚DNA。使pCP20从卡那霉素敏感性株脱落。将所得的菌株设为EcΔPP。

实施例5

导入有使丙酮酸激酶的功能受损、并且表达PEP羧化激酶和对反应A、B、E以及F进行催化的酶的质粒的大肠菌变异体的制作

对于实施例4所制作的EcΔPP，分别导入参考例1所制作的质粒，制作了大肠菌变异体。

将EcΔPP接种在5mL的LB培养基中，在30℃下振动培养1天。将0.5mL的培养液接种在5mL的LB培养基中，在30℃下振动培养2小时。将培养液冰冷20分钟后，使用10％(w/w)的丙三醇将菌体清洗3次。使用100μL的10％(w/w)的丙三醇将清洗后的团粒悬浊，与1μL的pBBR1MCS-2::ATCTOR1PCK、pBBR1MCS-2::ATCTOR2PCK、pBBR1MCS-2::ATCTOR3PCK、pBBR1MCS-2::ATCTOR4PCK、pBBR1MCS-2::ATCTOR5PCK、pBBR1MCS-2::ATCTOR6PCK或pBBR1MCS-2::ATCTOR7PCK混合后，在电穿孔液槽内冰冷10分钟。使用Gene pulser(Bio-rad公司制造)实施电穿孔(3kV、200Ω、25μF)后，立即添加1mL的SOC培养基，在30℃下振动培养1小时。将50μL涂布在包含25μg/mL卡那霉素的LB琼脂培养基上，在30℃下进行孵育1天。将所得的菌株分别设为EcΔPP/3HA1PCK、EcΔPP/3HA2PCK、EcΔPP/3HA3PCK、EcΔPP/3HA4PCK、EcΔPP/3HA5PCK、EcΔPP/3HA6PCK、EcΔPP/3HA7PCK。

参考例9

导入有不使丙酮酸激酶的功能受损、并且表达PEP羧化激酶和对反应A、B、E以及F进行催化的酶的质粒的大肠菌变异体的制作

通过与实施例5同样的方法，在Escherichia coli MG1655中导入pBBR1MCS-2::ATCTOR1PCK、pBBR1MCS-2::ATCTOR2PCK、pBBR1MCS-2::ATCTOR3PCK、pBBR1MCS-2::ATCTOR4PCK、pBBR1MCS-2::ATCTOR5PCK、pBBR1MCS-2::ATCTOR6PCK或pBBR1MCS-2::ATCTOR7PCK。另外，作为对照，制作了在Escherichia coli MG1655中导入有作为空白载体的pBBR1MCS-2的菌株。将所得的菌株分别设为Ec/3HA1PCK、Ec/3HA2PCK、Ec/3HA3PCK、Ec/3HA4PCK、Ec/3HA5PCK、Ec/3HA6PCK、Ec/3HA7PCK、Ec/pBBR(阴性对照)。

参考例10

导入有使丙酮酸激酶的功能受损、并且表达对反应A、B、E以及F进行催化的酶的质粒的大肠菌变异体的制作

通过与实施例5同样的方法，在EcΔPP中导入pBBR1MCS-2::ATCTOR1、pBBR1MCS-2::ATCTOR2、pBBR1MCS-2::ATCTOR3、pBBR1MCS-2::ATCTOR4、pBBR1MCS-2::ATCTOR5、pBBR1MCS-2::ATCTOR6或pBBR1MCS-2::ATCTOR7。另外，作为对照，制作了在EcΔPP中导入有作为空白载体的pBBR1MCS-2的菌株。将所得的菌株分别设为EcΔPP/3HA1、EcΔPP/3HA2、EcΔPP/3HA3、EcΔPP/3HA4、EcΔPP/3HA5、EcΔPP/3HA6、EcΔPP/3HA7、EcΔPP/pBBR(阴性对照)。

实施例6

利用使丙酮酸激酶的功能受损了的、并且强化了PEP羧化激酶的活性的大肠菌变异体生产3-羟基己二酸和α-氢化己二烯二酸的试验

通过与实施例3同样的方法，对实施例5中所制作的变异体进行培养。将培养上清液中积累的3-羟基己二酸、α-氢化己二烯二酸以及其他产物、培养基中未被利用而残存的糖类的浓度进行定量。根据该值并使用上式(2)算出的3-羟基己二酸和α-氢化己二烯二酸的产率如表6所示。

比较例3

利用未使丙酮酸激酶的功能受损、并且强化了PEP羧化激酶的活性的大肠菌变异体生产3-羟基己二酸和α-氢化己二烯二酸的试验

通过与实施例3同样的方法，对参考例9中所制作的变异体进行培养。将培养上清液中积累的3-羟基己二酸、α-氢化己二烯二酸以及其他产物、培养基中未被利用而残存的糖类的浓度进行定量。根据该值并使用上式(2)算出的3-羟基己二酸和α-氢化己二烯二酸的产率如表6所示。

比较例4

利用使丙酮酸激酶的功能受损了、并且未强化PEP羧化激酶的活性的大肠菌变异体生产3-羟基己二酸和α-氢化己二烯二酸的试验

通过与实施例3同样的方法，对参考例10中所制作的变异体进行培养。将培养上清液中积累的3-羟基己二酸、α-氢化己二烯二酸以及其他产物、培养基中未被利用而残存的糖类的浓度进行定量。进一步，根据该结果并使用上式(2)算出的3-羟基己二酸和α-氢化己二烯二酸的产率如表6所示。

当将比较例3和4的结果与实施例6的结果进行比较时，可知：通过使大肠菌的丙酮酸激酶的功能受损、并且强化PEP羧化激酶的活性，可以提高3-羟基己二酸和α-氢化己二烯二酸的产率。

[表6]

实施例7

导入有使丙酮酸激酶的功能受损、并且表达PEP羧化激酶和对反应A、B、C、E以及F进行催化的酶的质粒的沙雷氏菌属微生物变异体的制作

对于实施例2中所制作的沙雷氏菌属微生物变异体分别导入参考例2所制作的质粒pMW119::EH，制作了沙雷氏菌属微生物变异体。

将SgΔPP/3HA1PCK、SgΔPP/3HA2PCK、SgΔPP/3HA3PCK、SgΔPP/3HA4PCK、SgΔPP/3HA5PCK、SgΔPP/3HA6PCK、SgΔPP/3HA7PCK接种在包含25μg/mL卡那霉素的5mL的LB培养基中，在30℃下振动培养1天。将0.5mL的培养液接种在包含25μg/mL卡那霉素的5mL的LB培养基中，在30℃下振动培养2小时。将培养液冰冷20分钟后，使用10％(w/w)的丙三醇将菌体清洗3次。使用100μL的10％(w/w)的丙三醇将清洗后的团粒悬浊，与1μL的pMW119::EH混合后，在电穿孔液槽内冰冷10分钟。使用Gene pulser(Bio-rad公司制造)实施电穿孔(3kV、200Ω、25μF)后，立即添加1mL的SOC培养基，在30℃下振动培养1小时。将50μL涂布在包含500μg/mL的氨苄西林和25μg/mL的卡那霉素的LB琼脂培养基上，在30℃下进行孵育1天。将所得的菌株分别设为SgΔPP/HMA1PCK、SgΔPP/HMA2PCK、SgΔPP/HMA3PCK、SgΔPP/HMA4PCK、SgΔPP/HMA5PCK、SgΔPP/HMA6PCK、SgΔPP/HMA7PCK。

参考例11

导入有未使丙酮酸激酶的功能受损、并且表达PEP羧化激酶和对反应A、B、C、E以及F进行催化的酶的质粒的沙雷氏菌属微生物变异体的制作

通过与实施例7同样的方法，将pMW119::EH导入Sg/3HA1PCK、Sg/3HA2PCK、Sg/3HA3PCK、Sg/3HA4PCK、Sg/3HA5PCK、Sg/3HA6PCK、Sg/3HA7PCK中。另外，作为对照，制作了在Sg/pBBR中导入有作为空白载体的pMW119的菌株。将所得的菌株分别设为Sg/HMA1PCK、Sg/HMA2PCK、Sg/HMA3PCK、Sg/HMA4PCK、Sg/HMA5PCK、Sg/HMA6PCK、Sg/HMA7PCK、Sg/pBBRpMW(阴性对照)。

参考例12

导入有使丙酮酸激酶的功能受损、并且表达对反应A、B、C、E以及F进行催化的酶的质粒的沙雷氏菌属微生物变异体的制作

通过与实施例7同样的方法，将pMW119::EH导入SgΔPP/3HA1、SgΔPP/3HA2、SgΔPP/3HA3、SgΔPP/3HA4、SgΔPP/3HA5、SgΔPP/3HA6、SgΔPP/3HA7中。另外，作为对照，制作了在SgΔPP/pBBR中导入有作为空白载体的pMW119的菌株。将所得的菌株分别设为SgΔPP/HMA1、SgΔPP/HMA2、SgΔPP/HMA3、SgΔPP/HMA4、SgΔPP/HMA5、SgΔPP/HMA6、SgΔPP/HMA7、SgΔPP/pBBRpMW(阴性对照)。

实施例8

利用使丙酮酸激酶的功能受损了、并且强化了PEP羧化激酶的活性的沙雷氏菌属微生物变异体生产α-氢化己二烯二酸的试验

除了在培养基中添加500μg/mL的氨苄西林以外，通过与实施例3同样的方法，对实施例7中所制作的变异体进行培养。将培养上清液中积累的α-氢化己二烯二酸以及其他产物、培养基中未被利用而残存的糖类的浓度进行定量。根据该值并使用上式(2)算出的α-氢化己二烯二酸的产率如表7所示。

比较例5

利用未使丙酮酸激酶的功能受损、并且强化了PEP羧化激酶的活性的沙雷氏菌属微生物变异体生产α-氢化己二烯二酸的试验

通过与实施例8同样的方法，对参考例11中所制作的变异体进行培养。将培养上清液中积累的α-氢化己二烯二酸以及其他产物、培养基中未被利用而残存的糖类的浓度进行定量。根据该值并使用上式(2)算出的α-氢化己二烯二酸的产率如表7所示。

比较例6

利用使丙酮酸激酶的功能受损了、并且未强化PEP羧化激酶的活性的沙雷氏菌属微生物变异体生产α-氢化己二烯二酸的试验

通过与实施例8同样的方法，对参考例12中所制作的变异体进行培养。将培养上清液中积累的α-氢化己二烯二酸以及其他产物、培养基中未被利用而残存的糖类的浓度进行定量。进一步，根据该结果并使用上式(2)算出的α-氢化己二烯二酸的产率如表7所示。

当将比较例5和6的结果与实施例8的结果进行比较时，可知：通过使沙雷氏菌属微生物的丙酮酸激酶基因受损、并且强化PEP羧化激酶的活性，可以提高α-氢化己二烯二酸的产率。

[表7]

实施例9

导入有使丙酮酸激酶的功能受损、并且表达PEP羧化激酶和对反应A、B、C、E以及F进行催化的酶的质粒的大肠菌变异体的制作

对于实施例5所制作的大肠菌变异体分别导入参考例2所制作的质粒pMW119::EH，制作了大肠菌变异体。

将EcΔPP/3HA1PCK、EcΔPP/3HA2PCK、EcΔPP/3HA3PCK、EcΔPP/3HA4PCK、EcΔPP/3HA5PCK、EcΔPP/3HA6PCK、EcΔPP/3HA7PCK接种在包含25μg/mL的卡那霉素的5mL的LB培养基中，在30℃下振动培养1天。将0.5mL的培养液接种在包含25μg/mL的卡那霉素的5mL的LB培养基中，在30℃下振动培养2小时。将培养液冰冷20分钟后，使用10％(w/w)的丙三醇将菌体清洗3次。使用100μL的10％(w/w)的丙三醇将清洗后的团粒悬浊，与1μL的pMW119::EH混合后，在电穿孔液槽内冰冷10分钟。使用Gene pulser(Bio-rad公司制造)实施电穿孔(3kV、200Ω、25μF)后，立即添加1mL的SOC培养基，在30℃下振动培养1小时。将50μL涂布在包含100μg/mL氨苄西林和25μg/mL卡那霉素的LB琼脂培养基上，在30℃下进行孵育1天。将所得的菌株分别设为EcΔPP/HMA1PCK、EcΔPP/HMA2PCK、EcΔPP/HMA3PCK、EcΔPP/HMA4PCK、EcΔPP/HMA5PCK、EcΔPP/HMA6PCK、EcΔPP/HMA7PCK。

参考例13

导入有未使丙酮酸激酶的功能受损、并且表达PEP羧化激酶和对反应A、B、C、E以及F进行催化的酶的质粒的大肠菌变异体的制作

通过与实施例9同样的方法，将pMW119::EH导入Ec/3HA1PCK、Ec/3HA2PCK、Ec/3HA3PCK、Ec/3HA4PCK、Ec/3HA5PCK、Ec/3HA6PCK、Ec/3HA7PCK中。另外，作为对照，制作了在Ec/pBBR中导入有作为空白载体的pMW119的菌株。将所得的菌株分别设为Ec/HMA1PCK、Ec/HMA2PCK、Ec/HMA3PCK、Ec/HMA4PCK、Ec/HMA5PCK、Ec/HMA6PCK、Ec/HMA7PCK、Ec/pBBRpMW(阴性对照)。

参考例14

导入有使丙酮酸激酶的功能受损、并且表达对反应A、B、C、E以及F进行催化的酶的质粒的大肠菌变异体的制作

通过与实施例9同样的方法，将pMW119::EH导入EcΔPP/3HA1、EcΔPP/3HA2、EcΔPP/3HA3、EcΔPP/3HA4、EcΔPP/3HA5、EcΔPP/3HA6、EcΔPP/3HA7中。另外，作为对照，制作了在EcΔPP/pBBR中导入有作为空白载体的pMW119的菌株。将所得的菌株分别设为EcΔPP/HMA1、EcΔPP/HMA2、EcΔPP/HMA3、EcΔPP/HMA4、EcΔPP/HMA5、EcΔPP/HMA6、EcΔPP/HMA7、EcΔPP/pBBRpMW(阴性对照)。

实施例10

利用使丙酮酸激酶的功能受损了、并且强化了PEP羧化激酶的活性的大肠菌变异体生产α-氢化己二烯二酸的试验

除了在培养基中添加100μg/mL的氨苄西林以外，通过与实施例6同样的方法，对实施例9中所制作的变异体进行培养。将培养上清液中积累的α-氢化己二烯二酸以及其他产物、培养基中未被利用而残存的糖类的浓度进行定量。根据该值并使用上式(2)算出的α-氢化己二烯二酸的产率如表8所示。

比较例7

利用未使丙酮酸激酶的功能受损、并且强化了PEP羧化激酶的活性的大肠菌变异体生产α-氢化己二烯二酸的试验

通过与实施例10同样的方法，对参考例13中所制作的变异体进行培养。将培养上清液中积累的α-氢化己二烯二酸以及其他产物、培养基中未被利用而残存的糖类的浓度进行定量。根据该值并使用上式(2)算出的α-氢化己二烯二酸的产率如表8所示。

比较例8

利用使丙酮酸激酶的功能受损了、并且未强化PEP羧化激酶的活性的大肠菌变异体生产α-氢化己二烯二酸的试验

通过与实施例10同样的方法，对参考例14中所制作的变异体进行培养。将培养上清液中积累的α-氢化己二烯二酸以及其他产物、培养基中未被利用而残存的糖类的浓度进行定量。进一步，根据该结果并使用上式(2)算出的α-氢化己二烯二酸的产率如表8所示。

当将比较例7和8的结果与实施例10的结果进行比较时，可知：通过使大肠菌的丙酮酸激酶的功能受损、并且强化PEP羧化激酶的活性，可以提高α-氢化己二烯二酸的产率。

[表8]

实施例11

导入有使丙酮酸激酶的功能受损、并且表达PEP羧化激酶和对反应A、B、C、D以及G进行催化的酶的质粒的沙雷氏菌属微生物变异体的制作

通过与实施例2同样的方法，对SgΔPP导入了参考例6中所制作的pBBR1MCS-2::ATCT2OR1PCK、pBBR1MCS-2::ATCT2OR2PCK、pBBR1MCS-2::ATCT2OR3PCK、pBBR1MCS-2::ATCT2OR4PCK、pBBR1MCS-2::ATCT2OR5PCK、pBBR1MCS-2::ATCT2OR6PCK、或pBBR1MCS-2::ATCT2OR7PCK。通过与实施例7同样的方法，对所得的变异体分别导入参考例4中所制作的质粒pMW119::EHER，制作了沙雷氏菌属微生物变异体。将所得的菌株分别设为SgΔPP/ADA1PCK、SgΔPP/ADA2PCK、SgΔPP/ADA3PCK、SgΔPP/ADA4PCK、SgΔPP/ADA5PCK、SgΔPP/ADA6PCK、SgΔPP/ADA7PCK。

参考例15

导入有未使丙酮酸激酶的功能受损、并且表达PEP羧化激酶和对反应A、B、C、D以及G进行催化的酶的质粒的沙雷氏菌属微生物变异体的制作

通过与实施例2同样的方法，对Serratia grimesii NBRC13537导入了参考例6中所制作的pBBR1MCS-2::ATCT2OR1PCK、pBBR1MCS-2::ATCT2OR2PCK、pBBR1MCS-2::ATCT2OR3PCK、pBBR1MCS-2::ATCT2OR4PCK、pBBR1MCS-2::ATCT2OR5PCK、pBBR1MCS-2::ATCT2OR6PCK或pBBR1MCS-2::ATCT2OR7PCK。通过与实施例7同样的方法，对所得的变异体分别导入参考例4中所制作的质粒pMW119::EHER，制作了沙雷氏菌属微生物变异体。将所得的菌株分别设为Sg/ADA1PCK、Sg/ADA2PCK、Sg/ADA3PCK、Sg/ADA4PCK、Sg/ADA5PCK、Sg/ADA6PCK、Sg/ADA7PCK。

参考例16

导入有使丙酮酸激酶的功能受损、并且表达对反应A、B、C、D以及G进行催化的酶的质粒的沙雷氏菌属微生物变异体的制作

通过与实施例2同样的方法，对SgΔPP导入了参考例3中所制作的pBBR1MCS-2::ATCT2OR1、pBBR1MCS-2::ATCT2OR2、pBBR1MCS-2::ATCT2OR3、pBBR1MCS-2::ATCT2OR4、pBBR1MCS-2::ATCT2OR5、pBBR1MCS-2::ATCT2OR6或pBBR1MCS-2::ATCT2OR7。通过与实施例7同样的方法，对所得的变异体分别导入参考例4中所制作的质粒pMW119::EHER，制作了沙雷氏菌属微生物变异体。将所得的菌株分别设为SgΔPP/ADA1、SgΔPP/ADA2、SgΔPP/ADA3、SgΔPP/ADA4、SgΔPP/ADA5、SgΔPP/ADA6、SgΔPP/ADA7。

实施例12

利用使丙酮酸激酶的功能受损了、并且强化了PEP羧化激酶的活性的沙雷氏菌属微生物变异体生产己二酸的试验

除了在培养基中添加500μg/mL的氨苄西林以外，通过与实施例3同样的方法，对实施例11中所制作的变异体进行培养。对培养上清液中积累的己二酸以及其他产物、培养基中未被利用而残存的糖类的浓度进行定量。需要说明的是，己二酸的定量是通过使用LC-MS/MS在与3-羟基己二酸和α-氢化己二烯二酸同样的条件下进行的。根据该值并使用上式(2)算出的己二酸的产率如表9所示。

比较例9

利用未使丙酮酸激酶的功能受损、并且强化了PEP羧化激酶的活性的沙雷氏菌属微生物变异体生产己二酸的试验

通过与实施例12同样的方法，对参考例15中所制作的变异体和Sg/pBBRpMW进行培养。将培养上清液中积累的己二酸以及其他产物、培养基中未被利用而残存的糖类的浓度进行定量。根据该值并使用上式(2)算出的己二酸的产率如表9所示。

比较例10

利用使丙酮酸激酶的功能受损、并且未强化PEP羧化激酶的活性的沙雷氏菌属微生物变异体生产己二酸的试验

通过与实施例12同样的方法，对参考例16中所制作的变异体和SgΔPP/pBBRpMW进行培养。将培养上清液中积累的己二酸以及其他产物、培养基中未被利用而残存的糖类的浓度进行定量。进一步，根据该结果并使用上式(2)算出的己二酸的产率如表9所示。

当将比较例9和10的结果与实施例12的结果进行比较时，可知：通过使沙雷氏菌属微生物的丙酮酸激酶的功能受损、并且强化PEP羧化激酶的活性，可以提高己二酸的产率。

[表9]

实施例13

导入有使丙酮酸激酶的功能受损、并且表达PEP羧化激酶和对反应A、B、C、D以及G进行催化的酶的质粒的大肠菌变异体的制作

通过与实施例5同样的方法，对EcΔPP导入了参考例6中所制作的pBBR1MCS-2::ATCT2OR1PCK、pBBR1MCS-2::ATCT2OR2PCK、pBBR1MCS-2::ATCT2OR3PCK、pBBR1MCS-2::ATCT2OR4PCK、pBBR1MCS-2::ATCT2OR5PCK、pBBR1MCS-2::ATCT2OR6PCK或pBBR1MCS-2::ATCT2OR7PCK。通过与实施例9同样的方法，对所得的变异体分别导入参考例4中所制作的质粒pMW119::EHER，制作了大肠菌变异体。将所得的菌株分别设为EcΔPP/ADA1PCK、EcΔPP/ADA2PCK、EcΔPP/ADA3PCK、EcΔPP/ADA4PCK、EcΔPP/ADA5PCK、EcΔPP/ADA6PCK、EcΔPP/ADA7PCK。

参考例17

导入有未使丙酮酸激酶的功能受损、并且表达PEP羧化激酶和对反应A、B、C、D以及G进行催化的酶的质粒的大肠菌变异体的制作

通过与实施例5同样的方法，对Escherichia coli MG1655导入了参考例6中所制作的pBBR1MCS-2::ATCT2OR1PCK、pBBR1MCS-2::ATCT2OR2PCK、pBBR1MCS-2::ATCT2OR3PCK、pBBR1MCS-2::ATCT2OR4PCK、pBBR1MCS-2::ATCT2OR5PCK、pBBR1MCS-2::ATCT2OR6PCK或pBBR1MCS-2::ATCT2OR7PCK。通过与实施例9同样的方法，对所得的变异体分别导入参考例4中所制作的质粒pMW119::EHER，制作了变异体。将所得的菌株分别设为Ec/ADA1PCK、Ec/ADA2PCK、Ec/ADA3PCK、Ec/ADA4PCK、Ec/ADA5PCK、Ec/ADA6PCK、Ec/ADA7PCK。

参考例18

导入有使丙酮酸激酶的功能受损、并且表达对反应A、B、C、D以及G进行催化的酶的质粒的大肠菌变异体的制作

通过与实施例5同样的方法，对EcΔPP导入了参考例3中所制作的pBBR1MCS-2::ATCT2OR1、pBBR1MCS-2::ATCT2OR2、pBBR1MCS-2::ATCT2OR3、pBBR1MCS-2::ATCT2OR4、pBBR1MCS-2::ATCT2OR5、pBBR1MCS-2::ATCT2OR6或pBBR1MCS-2::ATCT2OR7。通过与实施例9同样的方法，对所得的变异体分别导入参考例4中所制作的质粒pMW119::EHER，制作了变异体。将所得的菌株分别设为EcΔPP/ADA1、EcΔPP/ADA2、EcΔPP/ADA3、EcΔPP/ADA4、EcΔPP/ADA5、EcΔPP/ADA6、EcΔPP/ADA7。

实施例14

利用使丙酮酸激酶的功能受损了、并且强化了PEP羧化激酶的活性的大肠菌变异体生产己二酸的试验

除了在培养基中添加500μg/mL的氨苄西林以外，通过与实施例6同样的方法，对实施例13中所制作的变异体进行培养。对培养上清液中积累的己二酸以及其他产物、培养基中未被利用而残存的糖类的浓度进行定量。需要说明的是，己二酸的定量是通过使用LC-MS/MS在与3-羟基己二酸和α-氢化己二烯二酸同样的条件下进行的。根据该值并使用上式(2)算出的己二酸的产率如表10所示。

比较例11

利用未使丙酮酸激酶的功能受损、并且强化了PEP羧化激酶的活性的大肠菌变异体生产己二酸的试验

通过与实施例14同样的方法，对参考例17中所制作的变异体进行培养。将培养上清液中积累的己二酸以及其他产物、培养基中未被利用而残存的糖类的浓度进行定量。根据该值并使用上式(2)算出的己二酸的产率如表10所示。

比较例12

利用使丙酮酸激酶的功能受损、并且未强化PEP羧化激酶的活性的大肠菌变异体生产己二酸的试验

通过与实施例14同样的方法，对参考例18中所制作的变异体进行培养。将培养上清液中积累的己二酸以及其他产物、培养基中未被利用而残存的糖类的浓度进行定量。进一步，根据该结果并使用上式(2)算出的己二酸的产率如表10所示。

当将比较例11和12的结果与实施例14的结果进行比较时，可知：通过使大肠菌的丙酮酸激酶的功能受损、并且强化PEP羧化激酶的活性，可以提高己二酸的产率。

[表10]

实施例15

利用使丙酮酸激酶的功能受损了、并且强化了PEP羧化激酶的活性的沙雷氏菌属微生物变异体生产3-羟基己二酸和α-氢化己二烯二酸的试验2

利用实施例2中所制作的沙雷氏菌属微生物变异体，在厌氧条件下进行3-羟基己二酸和α-氢化己二烯二酸的生产试验。

除了在静置下使用培养基II进行培养以外，通过与实施例3同样的方法，对实施例2中所制作的沙雷氏菌属微生物变异体进行培养。对培养上清液中积累的3-羟基己二酸、α-氢化己二烯二酸以及其他产物、培养基中未被利用而残存的糖类的浓度进行定量。根据该值并使用上式(2)算出的3-羟基己二酸和α-氢化己二烯二酸的产率如表11所示。

比较例13

利用未使丙酮酸激酶的功能受损、并且强化了PEP羧化激酶的活性的沙雷氏菌属微生物变异体生产3-羟基己二酸和α-氢化己二烯二酸的试验2

通过与实施例15同样的方法，对参考例7中所制作的变异体进行培养。对培养上清液中积累的3-羟基己二酸、α-氢化己二烯二酸以及其他产物、培养基中未被利用而残存的糖类的浓度进行定量。根据该值并使用上式(2)算出的3-羟基己二酸和α-氢化己二烯二酸的产率如表11所示。

比较例14

利用使丙酮酸激酶的功能受损了、并且未强化PEP羧化激酶的活性的沙雷氏菌属微生物变异体生产3-羟基己二酸和α-氢化己二烯二酸的试验2

通过与实施例15同样的方法，对参考例8中所制作的变异体进行培养。对培养上清液中积累的3-羟基己二酸、α-氢化己二烯二酸以及其他产物、培养基中未被利用而残存的糖类的浓度进行定量。根据该值并使用上式(2)算出的3-羟基己二酸和α-氢化己二烯二酸的产率如表11所示。

当将比较例13和14的结果与实施例15的结果进行比较时，可知：通过使沙雷氏菌属微生物的丙酮酸激酶的功能受损、并且强化PEP羧化激酶的活性，即使在厌氧条件下也可以提高3-羟基己二酸和α-氢化己二烯二酸的产率。

[表11]

实施例16

利用使丙酮酸激酶的功能受损了的大肠菌变异体生产3-羟基己二酸和α-氢化己二烯二酸的试验2

利用实施例5中所制作的大肠菌变异体，在厌氧条件下进行3-羟基己二酸和α-氢化己二烯二酸的生产试验。

除了在静置下使用培养基II进行培养以外，通过与实施例6同样的方法，对实施例5中所制作的大肠菌变异体进行培养。对培养上清液中积累的3-羟基己二酸、α-氢化己二烯二酸以及其他产物、培养基中未被利用而残存的糖类的浓度进行定量。根据该值并使用上式(2)算出的3-羟基己二酸和α-氢化己二烯二酸的产率如表12所示。

比较例15

利用未使丙酮酸激酶的功能受损、并且强化了PEP羧化激酶的活性的大肠菌变异体生产3-羟基己二酸和α-氢化己二烯二酸的试验2

通过与实施例16同样的方法，对参考例9中所制作的变异体进行培养。对培养上清液中积累的3-羟基己二酸、α-氢化己二烯二酸以及其他产物、培养基中未被利用而残存的糖类的浓度进行定量。根据该值并使用上式(2)算出的3-羟基己二酸和α-氢化己二烯二酸的产率如表12所示。

比较例16

利用使丙酮酸激酶的功能受损了、并且未强化PEP羧化激酶的活性的大肠菌变异体生产3-羟基己二酸和α-氢化己二烯二酸的试验2

通过与实施例16同样的方法，对参考例10中所制作的变异体进行培养。对培养上清液中积累的3-羟基己二酸、α-氢化己二烯二酸以及其他产物、培养基中未被利用而残存的糖类的浓度进行定量。根据该值并使用上式(2)算出的3-羟基己二酸和α-氢化己二烯二酸的产率如表12所示。

当将比较例15和16的结果与实施例16的结果进行比较时，可知：通过使大肠菌的丙酮酸激酶的功能受损、并且强化PEP羧化激酶的活性，即使在厌氧条件下也可以提高3-羟基己二酸和α-氢化己二烯二酸的产率。

[表12]

实施例17

利用使丙酮酸激酶的功能受损了、并且强化了PEP羧化激酶的活性的沙雷氏菌属微生物变异体生产己二酸的试验2

利用实施例11中所制作的沙雷氏菌属微生物变异体，在厌氧条件下进行己二酸的生产试验。

除了在静置下使用培养基II进行培养以外，通过与实施例12同样的方法，对实施例11中所制作的沙雷氏菌属微生物变异体进行培养。对培养上清液中积累的己二酸以及其他产物、培养基中未被利用而残存的糖类的浓度进行定量。根据该值并使用上式(2)算出的己二酸的产率如表13所示。

比较例17

利用未使丙酮酸激酶的功能受损、并且强化了PEP羧化激酶的活性的沙雷氏菌属微生物变异体生产己二酸的试验2

通过与实施例17同样的方法，对参考例15中所制作的变异体进行培养。对培养上清液中积累的己二酸以及其他产物、培养基中未被利用而残存的糖类的浓度进行定量。根据该值并使用上式(2)算出的己二酸的产率如表13所示。

比较例18

利用使丙酮酸激酶的功能受损了、并且未强化PEP羧化激酶的活性的沙雷氏菌属微生物变异体生产己二酸的试验2

通过与实施例17同样的方法，对参考例16中所制作的变异体进行培养。对培养上清液中积累的己二酸以及其他产物、培养基中未被利用而残存的糖类的浓度进行定量。根据该值并使用上式(2)算出的己二酸的产率如表13所示。

当将比较例17和18的结果与实施例17的结果进行比较时，可知：通过使沙雷氏菌属微生物的丙酮酸激酶的功能受损、并且强化PEP羧化激酶的活性，即使在厌氧条件下也可以提高己二酸的产率。

[表13]

实施例18

利用使丙酮酸激酶的功能受损了的大肠菌变异体生产己二酸的试验2

利用实施例13中所制作的大肠菌变异体，在厌氧条件下进行己二酸的生产试验。除了在静置下使用培养基II进行培养以外，通过与实施例14同样的方法，对实施例13中所制作的大肠菌变异体进行培养。对培养上清液中积累的己二酸以及其他产物、培养基中未被利用而残存的糖类的浓度进行定量。根据该值并使用上式(2)算出的己二酸的产率如表14所示。

比较例19

利用未使丙酮酸激酶的功能受损、并且强化了PEP羧化激酶的活性的大肠菌变异体生产己二酸的试验2

通过与实施例18同样的方法，对参考例17中所制作的变异体进行培养。对培养上清液中积累的己二酸以及其他产物、培养基中未被利用而残存的糖类的浓度进行定量。根据该值并使用上式(2)算出的己二酸的产率如表14所示。

比较例20

利用使丙酮酸激酶的功能受损了、并且未强化PEP羧化激酶的活性的大肠菌变异体生产己二酸的试验2

通过与实施例18同样的方法，对参考例18中所制作的变异体进行培养。对培养上清液中积累的己二酸以及其他产物、培养基中未被利用而残存的糖类的浓度进行定量。根据该值并使用上式(2)算出的己二酸的产率如表14所示。

当将比较例19和20的结果与实施例18的结果进行比较时，可知：通过使大肠菌的丙酮酸激酶的功能受损、并且强化PEP羧化激酶的活性，即使在厌氧条件下也可以提高己二酸的产率。

[表14]

参考例19

导入有不使丙酮酸激酶的功能受损、并且表达对反应A、B、E以及F进行催化的酶的质粒的沙雷氏菌属微生物变异体的制作

通过与实施例2同样的方法，在Serratia grimesii NBRC 13537中导入pBBR1MCS-2(对照)、pBBR1MCS-2::ATCTOR1、pBBR1MCS-2::ATCTOR2、pBBR1MCS-2::ATCTOR3、pBBR1MCS-2::ATCTOR4、pBBR1MCS-2::ATCTOR5、pBBR1MCS-2::ATCTOR6或pBBR1MCS-2::ATCTOR7。将所得的菌株分别设为Sg/pBBR(阴性对照)、Sg/3HA1、Sg/3HA2、Sg/3HA3、Sg/3HA4、Sg/3HA5、Sg/3HA6、Sg/3HA7。

比较例21

利用未使丙酮酸激酶受损的沙雷氏菌属微生物变异体生产3-羟基己二酸和α-氢化己二烯二酸的试验

通过与实施例3同样的方法，对参考例19所制作的沙雷氏菌属微生物变异体进行培养。对培养上清液中积累的3-羟基己二酸、α-氢化己二烯二酸以及其他产物、培养基中未被利用而残存的糖类的浓度进行定量。进一步，根据该结果并使用上式(2)算出的3-羟基己二酸和α-氢化己二烯二酸的产率如表15所示。

当将本比较例21的结果与比较例1的结果进行比较时，可知：对于未使丙酮酸激酶的功能受损的沙雷氏菌属微生物而言，强化了PEP羧化激酶的活性的情况与未强化的情况相比，3-羟基己二酸和α-氢化己二烯二酸的产率降低。

[表15]

参考例20

导入有不使丙酮酸激酶的功能受损、并且表达对反应A、B、E以及F进行催化的酶的质粒的大肠菌变异体的制作

通过与实施例5同样的方法，在Escherichia coli MG1655中导入pBBR1MCS-2(对照)、pBBR1MCS-2::ATCTOR1、pBBR1MCS-2::ATCTOR2、pBBR1MCS-2::ATCTOR3、pBBR1MCS-2::ATCTOR4、pBBR1MCS-2::ATCTOR5、pBBR1MCS-2::ATCTOR6或pBBR1MCS-2::ATCTOR7。将所得的菌株分别设为Ec/pBBR(阴性对照)、Ec/3HA1、Ec/3HA2、Ec/3HA3、Ec/3HA4、Ec/3HA5、Ec/3HA6、Ec/3HA7。

比较例22

利用未使丙酮酸激酶的功能受损的大肠菌变异体生产3-羟基己二酸和α-氢化己二烯二酸的试验

通过与实施例6同样的方法，对参考例20中所制作的变异体进行培养。将培养上清液中积累的3-羟基己二酸、α-氢化己二烯二酸以及其他产物、培养基中未被利用而残存的糖类的浓度进行定量。根据该值并使用上式(2)算出的3-羟基己二酸和α-氢化己二烯二酸的产率如表16所示。

当将本比较例22的结果与比较例3的结果进行比较时，可知：对于未使丙酮酸激酶的功能受损的大肠菌而言，强化了PEP羧化激酶的活性的情况与未强化的情况相比，3-羟基己二酸和α-氢化己二烯二酸的产率降低。

[表16]

参考例21

导入有未使丙酮酸激酶的功能受损、并且表达对反应A、B、C、E以及F进行催化的酶的质粒的沙雷氏菌属微生物变异体的制作

通过与实施例7同样的方法，将pMW119(对照)或pMW119::EH导入Sg/pBBR、Sg/3HA1、Sg/3HA2、Sg/3HA3、Sg/3HA4、Sg/3HA5、Sg/3HA6、Sg/3HA7中。将所得的菌株分别设为Sg/pBBRpMW(阴性对照)、Sg/HMA1、Sg/HMA2、Sg/HMA3、Sg/HMA4、Sg/HMA5、Sg/HMA6、Sg/HMA7。

比较例23

利用未使丙酮酸激酶的功能受损的沙雷氏菌属微生物变异体生产α-氢化己二烯二酸的试验

通过与实施例8同样的方法，对参考例21中所制作的变异体进行培养。将培养上清液中积累的α-氢化己二烯二酸以及其他产物、培养基中未被利用而残存的糖类的浓度进行定量。根据该值并使用上式(2)算出的α-氢化己二烯二酸的产率如表17所示。

当将本比较例23的结果与比较例5的结果进行比较时，可知：对于未使丙酮酸激酶的功能受损的沙雷氏菌属微生物而言，强化了PEP羧化激酶的活性的情况与未强化的情况相比，α-氢化己二烯二酸的产率降低。

[表17]

参考例22

导入有未使丙酮酸激酶的功能受损、并且表达对反应A、B、C、E以及F进行催化的酶的质粒的大肠菌变异体的制作

通过与实施例9同样的方法，将pMW119(对照)或pMW119::EH导入Ec/pBBR、Ec/3HA1、Ec/3HA2、Ec/3HA3、Ec/3HA4、Ec/3HA5、Ec/3HA6、Ec/3HA7中。将所得的菌株分别设为Ec/pBBRpMW(阴性对照)、Ec/HMA1、Ec/HMA2、Ec/HMA3、Ec/HMA4、Ec/HMA5、Ec/HMA6、Ec/HMA7。

比较例24

利用未使丙酮酸激酶的功能受损的大肠菌变异体生产α-氢化己二烯二酸的试验

通过与实施例10同样的方法，对参考例22中所制作的变异体进行培养。将培养上清液中积累的α-氢化己二烯二酸以及其他产物、培养基中未被利用而残存的糖类的浓度进行定量。根据该值并使用上式(2)算出的α-氢化己二烯二酸的产率如表18所示。

当将本比较例24的结果与比较例7的结果进行比较时，可知：对于未使丙酮酸激酶的功能受损的大肠菌而言，强化了PEP羧化激酶的活性的情况与未强化的情况相比，α-氢化己二烯二酸的产率降低。

[表18]

参考例23

导入有未使丙酮酸激酶的功能受损、并且表达对反应A、B、C、D以及G进行催化的酶的质粒的沙雷氏菌属微生物变异体的制作

通过与实施例11同样的方法，对Serratia grimesii NBRC13537导入了参考例3中所制作的pBBR1MCS-2::ATCT2OR1、pBBR1MCS-2::ATCT2OR2、pBBR1MCS-2::ATCT2OR3、pBBR1MCS-2::ATCT2OR4、pBBR1MCS-2::ATCT2OR5、pBBR1MCS-2::ATCT2OR6或pBBR1MCS-2::ATCT2OR7。通过与实施例7同样的方法，对所得的变异体分别导入参考例4中所制作的质粒pMW119::EHER，制作了沙雷氏菌属微生物变异体。将所得的菌株分别设为Sg/ADA1、Sg/ADA2、Sg/ADA3、Sg/ADA4、Sg/ADA5、Sg/ADA6、Sg/ADA7。

比较例25

利用未使丙酮酸激酶的功能受损的沙雷氏菌属微生物变异体生产己二酸的试验

通过与实施例8同样的方法，对参考例23中所制作的变异体和Sg/pBBRpMW进行培养。将培养上清液中积累的己二酸以及其他产物、培养基中未被利用而残存的糖类的浓度进行定量。根据该值并使用上式(2)算出的己二酸的产率如表19所示。

当将本比较例25的结果与比较例9的结果进行比较时，可知：对于未使丙酮酸激酶的功能受损的沙雷氏菌属微生物而言，强化了PEP羧化激酶的活性的情况与未强化的情况相比，己二酸的产率降低。

[表19]

参考例24

导入有未使丙酮酸激酶的功能受损、并且表达对反应A、B、C、D以及G进行催化的酶的质粒的大肠菌变异体的制作

通过与实施例13同样的方法，对Escherichia coli MG1655导入了参考例3中所制作的pBBR1MCS-2::ATCT2OR1、pBBR1MCS-2::ATCT2OR2、pBBR1MCS-2::ATCT2OR3、pBBR1MCS-2::ATCT2OR4、pBBR1MCS-2::ATCT2OR5、pBBR1MCS-2::ATCT2OR6或pBBR1MCS-2::ATCT2OR7。通过与实施例9同样的方法，对所得的变异体分别导入参考例4中所制作的质粒pMW119::EHER，制作了大肠菌变异体。将所得的菌株分别设为Ec/ADA1、Ec/ADA2、Ec/ADA3、Ec/ADA4、Ec/ADA5、Ec/ADA6、Ec/ADA7。

比较例26

利用未使丙酮酸激酶的功能受损的大肠菌变异体生产己二酸的试验

通过与实施例10同样的方法，对参考例24中所制作的变异体和Ec/pBBRpMW进行培养。将培养上清液中积累的己二酸以及其他产物、培养基中未被利用而残存的糖类的浓度进行定量。根据该值并使用上式(2)算出的己二酸的产率如表20所示。

当将本比较例26的结果与比较例11的结果进行比较时，可知：对于未使丙酮酸激酶的功能受损的大肠菌而言，强化了PEP羧化激酶的活性的情况与未强化的情况相比，己二酸的产率降低。

[表20]

比较例27

利用未使丙酮酸激酶的功能受损的沙雷氏菌属微生物变异体生产3-羟基己二酸和α-氢化己二烯二酸的试验2

通过与实施例15同样的方法，对参考例19中所制作的变异体进行培养。对培养上清液中积累的3-羟基己二酸、α-氢化己二烯二酸以及其他产物、培养基中未被利用而残存的糖类的浓度进行定量。根据该值并使用上式(2)算出的3-羟基己二酸和α-氢化己二烯二酸的产率如表21所示。

当将本比较例27的结果与比较例13的结果进行比较时，可知：对于未使丙酮酸激酶的功能受损的沙雷氏菌属微生物而言，强化了PEP羧化激酶的活性的情况与未强化的情况相比，即使在厌氧条件下，3-羟基己二酸和α-氢化己二烯二酸的产率也降低。

[表21]

比较例28

利用未使丙酮酸激酶的功能受损的大肠菌变异体生产3-羟基己二酸和α-氢化己二烯二酸的试验2

通过与实施例16同样的方法，对参考例20中所制作的变异体进行培养。对培养上清液中积累的3-羟基己二酸、α-氢化己二烯二酸以及其他产物、培养基中未被利用而残存的糖类的浓度进行定量。根据该值并使用上式(2)算出的3-羟基己二酸和α-氢化己二烯二酸的产率如表22所示。

当将本比较例28的结果与比较例15的结果进行比较时，可知：对于未使丙酮酸激酶的功能受损的大肠菌而言，强化了PEP羧化激酶的活性的情况与未强化的情况相比，即使在厌氧条件下，3-羟基己二酸和α-氢化己二烯二酸的产率也降低。

[表22]

比较例29

利用未使丙酮酸激酶的功能受损的沙雷氏菌属微生物变异体生产己二酸的试验2

通过与实施例17同样的方法，对参考例23中所制作的变异体进行培养。对培养上清液中积累的己二酸以及其他产物、培养基中未被利用而残存的糖类的浓度进行定量。根据该值并使用上式(2)算出的己二酸的产率如表23所示。

当将本比较例29的结果与比较例17的结果进行比较时，可知：对于未使丙酮酸激酶的功能受损的沙雷氏菌属微生物而言，强化了PEP羧化激酶的活性的情况与未强化的情况相比，即使在厌氧条件下，己二酸的产率也降低。

[表23]

比较例30

利用未使丙酮酸激酶的功能受损的大肠菌变异体生产己二酸的试验2

通过与实施例18同样的方法，对参考例24中所制作的变异体进行培养。对培养上清液中积累的己二酸以及其他产物、培养基中未被利用而残存的糖类的浓度进行定量。根据该值并使用上式(2)算出的己二酸的产率如表24所示。

当将本比较例30的结果与比较例19的结果进行比较时，可知：对于未使丙酮酸激酶的功能受损的大肠菌而言，强化了PEP羧化激酶的活性的情况与未强化的情况相比，即使在厌氧条件下，己二酸的产率也降低。

[表24]

实施例19

使丙酮酸激酶基因和磷酸转移酶类酶基因受损了的沙雷氏菌属微生物的基因变异体的制作

使对实施例1中制作的SgΔPP株所具有的磷酸转移酶进行编码的基因ptsG受损，从而制作了使丙酮酸激酶和磷酸转移酶类酶的功能受损了的沙雷氏菌属微生物变异体。

以pKD4为模板，使用序列号244和245的寡聚DNA作为引物来进行PCR，得到用于使ptsG受损的序列长度1.6kb的PCR片段。在该菌株中导入pKD46后，导入pstsG受损用PCR片段。使用所得的卡那霉素耐性株进行菌落直接PCR，根据条带长度确认了目的基因的受损和卡那霉素耐性基因的插入。引物使用了序列号224和247的寡聚DNA。

接着，使pKD46脱落，得到氨苄西林敏感性株。在氨苄西林敏感性株中导入pCP20，再次获得氨苄西林耐性株。使用所得的菌株进行菌落直接PCR，根据条带长度确认了卡那霉素耐性基因的脱落。引物使用了序列号246和247的寡聚DNA。使pCP20从卡那霉素敏感性株脱落。将所得的菌株以下记为SgΔPPG。

实施例20

导入有使丙酮酸激酶基因和磷酸转移酶类酶基因受损、并且表达PEP羧化激酶和对反应A、B、E以及F进行催化的酶的质粒的沙雷氏菌属微生物的基因变异体的制作

通过与实施例2同样的方法，对实施例19所制作的SgΔPPG株导入参考例5中所制作的质粒pBBR1MCS-2::ATCTOR1PCK，将所得的沙雷氏菌属微生物变异体设为SgΔPPG/3HA1PCK。

实施例21

使用导入有使丙酮酸激酶和磷酸转移酶类酶的功能受损、并且表达PEP羧化激酶和对反应A、B、E以及F进行催化的酶的质粒的沙雷氏菌属微生物变异体，生产3-羟基己二酸和α-氢化己二烯二酸的试验

使用实施例20中所制作的沙雷氏菌属微生物变异体，通过与实施例15同样的方法，进行了3-羟基己二酸和α-氢化己二烯二酸的生产试验。

比较例31

使用导入有未使丙酮酸激酶和磷酸转移酶类酶的功能受损、并且表达PEP羧化激酶和对反应A、B、E以及F进行催化的酶的质粒的沙雷氏菌属微生物变异体，生产3-羟基己二酸和α-氢化己二烯二酸的试验

使用参考例7中所制作的Sg/3HA1PCK株，通过与比较例13同样的方法进行了3-羟基己二酸和α-氢化己二烯二酸的生产试验。

在将实施例21的结果与实施例15的结果进行比较时，可知：在使丙酮酸激酶和磷酸转移酶类酶的基因受损、并且强化了PEP羧化激酶和对将3-氧代己二酰-CoA还原生成3-羟基己二酰-CoA的反应进行催化的酶活性的沙雷氏菌属微生物变异体中，3-羟基己二酸和α-氢化己二烯二酸的产率进一步提高。另外，在将实施例21与比较例31的结果进行比较时，可知：在使丙酮酸激酶和磷酸转移酶类酶的基因受损了的变异体中，乙酰-CoA转化生成的乙酸和乙醇的产率也提高。

[表25]

实施例22

使丙酮酸激酶基因和磷酸转移酶类酶基因受损了的大肠菌变异体的制作

使对实施例4中所制作的EcΔPP株所具有的磷酸转移酶进行编码的基因ptsG受损，从而制作了使丙酮酸激酶和磷酸转移酶类酶的功能受损了的大肠菌变异体。

以pKD4为模板，使用序列号248和249的寡聚DNA作为引物来进行PCR，得到用于使ptsG受损的序列长度1.6kb的PCR片段。在该菌株中导入pKD46后，导入pstsG受损用PCR片段。使用所得的卡那霉素耐性株进行菌落直接PCR，根据条带长度确认了目的基因的受损和卡那霉素耐性基因的插入。引物使用了序列号224和251的寡聚DNA。

接着，使pKD46脱落，得到氨苄西林敏感性株。在氨苄西林敏感性株中导入pCP20，再次获得氨苄西林耐性株。使用所得的菌株进行菌落直接PCR，根据条带长度确认了卡那霉素耐性基因的脱落。引物使用了序列号250和251的寡聚DNA。使pCP20从卡那霉素敏感性株脱落。将所得的菌株以下记为EcΔPPG。

实施例23

导入有使丙酮酸激酶基因和磷酸转移酶类酶基因受损、并且表达PEP羧化激酶和对反应A、B、E以及F进行催化的酶的质粒的大肠菌变异体的制作

通过与实施例5同样的方法，对实施例22中所制作的EcΔPPG株导入参考例5中所制作的pBBR1MCS-2::ATCTOR1PCK，将所得的大肠菌变异体设为EcΔPPG/3HA1PCK。

实施例24

使用导入有使丙酮酸激酶和磷酸转移酶类酶的功能受损、并且表达PEP羧化激酶和对反应A、B、E以及F进行催化的酶的质粒的大肠菌变异体，生产3-羟基己二酸和α-氢化己二烯二酸的试验

使用实施例23中所制作的大肠菌变异体，通过与实施例16同样的方法，进行了3-羟基己二酸和α-氢化己二烯二酸的生产试验。

比较例32

使用导入有未使丙酮酸激酶和磷酸转移酶类酶的功能受损、并且表达PEP羧化激酶和对反应A、B、E以及F进行催化的酶的质粒的大肠菌变异体，生产3-羟基己二酸和α-氢化己二烯二酸的试验

使用参考例9中所制作的Ec/3HA1，通过与比较例15同样的方法进行了3-羟基己二酸和α-氢化己二烯二酸的生产试验。

在将实施例24的结果与实施例16的结果进行比较时，可知：在使丙酮酸激酶和磷酸转移酶类酶的基因受损、并且强化了对将3-氧代己二酰-CoA还原生成3-羟基己二酰-CoA的反应进行催化的酶活性的大肠菌变异体中，3-羟基己二酸和α-氢化己二烯二酸的产率进一步提高。另外，在将实施例24与比较例32的结果进行比较时，可知：在使丙酮酸激酶和磷酸转移酶类酶的基因受损了的变异体中，乙酰-CoA转化生成的乙酸和乙醇的产率也提高。

[表26]

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Ile Asp Ile Ala Ala Val Ala Gly Val Asn Tyr Ala Asp Gly Ile Phe

450 455 460

Ser Trp Leu Ala Gln Leu Gly Gln Lys Asn Val Lys Ser Thr Leu Asp

465 470 475 480

Asn Met Ala Gln Leu Leu His Ser Ala Arg Tyr Tyr Pro His Tyr Ser

485 490 495

Leu Leu Asn Thr Pro Arg Pro Glu Leu Ala Val Ala Pro

500 505

<210> 5

<211> 509

<212> PRT

<213> Serratia ureilytica Lr5/4

<400> 5

Met Ala Glu Ser Asn Ala Ala Ile Gln Ser Ala Ala Ile Ile Gly Ala

1 5 10 15

Gly Thr Met Gly Arg Gly Ile Ala Tyr Leu Leu Ala Gln Lys Ser Ile

20 25 30

Arg Thr Val Leu Tyr Asn Arg Asn Gly Asn Thr Leu Asn Gln Ala Arg

35 40 45

Asp Ala Ile Val Gln Asp Leu Asn Lys Lys Val Glu Gln Gly Lys Leu

50 55 60

Ala Leu Gln Asp Lys Asp Ala Val Leu Ala Asn Leu Thr Phe Ser Ala

65 70 75 80

Glu Phe Gly Ala Ile Ala Asp Ser Asp Leu Val Ile Glu Thr Ile Ala

85 90 95

Glu His Glu Gln Ala Lys Leu Glu Val Leu Ala Ala Ile Ala Ala Thr

100 105 110

Val Lys Pro Asp Thr Leu Ile Ala Thr Asn Thr Ser Ser Leu Ser Leu

115 120 125

Asn Lys Leu Ala Thr Ala Val Thr His Ser Glu Arg Phe Ile Gly Leu

130 135 140

His Phe Phe Asn Pro Ala Pro Leu Met Lys Leu Ile Glu Ile Ile Pro

145 150 155 160

Ala Tyr Phe Thr Ala Gln Val Thr Thr Glu Arg Cys Arg Gln Leu Val

165 170 175

Ala Ala Leu Gly Lys Arg Asp Val Val Cys Gln Ala Thr Pro Gly Phe

180 185 190

Ile Val Asn Arg Met Ala Arg Pro Tyr Tyr Leu Glu Gly Phe Arg Leu

195 200 205

Leu Glu Glu His Val Ala Arg Ala Pro Gln Ile Asp Arg Ala Leu Lys

210 215 220

Ala Gly Gly His Phe Arg Met Gly Pro Leu Glu Leu Thr Asp Phe Ile

225 230 235 240

Gly Gln Asp Ile Asn Tyr Gln Val Ser Arg Gln Ile Trp Gln Asp Met

245 250 255

Gln Tyr Asp Pro Arg Tyr Thr Pro Gly His Leu Gln Arg Ser Leu Val

260 265 270

Asp Ala Gly Leu Leu Gly Lys Lys Asn Gly Arg Ser Tyr Phe Ser Ala

275 280 285

Glu Glu Ser Pro Pro Pro Leu Ala Ala Ala Val Asp Ala Glu Val Glu

290 295 300

Thr Leu Arg Ile Tyr Gly Glu His Pro Leu Phe Thr Leu Leu Gln Gln

305 310 315 320

Arg Ala Thr Leu Gln Trp Pro Arg Leu Arg Val Glu Gln Arg Pro Thr

325 330 335

Leu Pro Gly Leu Gly Ala Ala Ile Gln Val Asn Asp Ala Phe Thr Val

340 345 350

Ser Val Thr Asp Gly Arg Thr Ala Asn Gln Leu Ala Glu Gln Thr Ala

355 360 365

Ala Asp Ala Phe Val Val Asp Val Ala Leu Asn Tyr Gly Asp Thr Ala

370 375 380

Tyr Leu Val Ala Ala His Ser Arg His Ala Ser Ala Ala Asn Lys Ala

385 390 395 400

Leu Phe Leu Arg Leu Leu His Thr Ala Ile Pro Gln Val Glu Phe Ile

405 410 415

Lys Asp Ser Pro Ala Leu Ile Val Ala Arg Val Leu Ser Ser Leu Ile

420 425 430

Asn Glu Ser Val Ile Met Val Glu Ser Gly Val Cys Ser Arg Glu Asp

435 440 445

Ile Asp Val Ala Ala Val Ala Gly Val Asn Tyr Ala Asp Gly Ile Phe

450 455 460

Gly Trp Leu Thr Arg Leu Gly Glu Glu Asn Val Arg Thr Thr Leu Ser

465 470 475 480

Asn Leu Ala Gln Leu Leu His Ala Ala Arg Tyr Ala Pro His Tyr Thr

485 490 495

Leu Leu His Ala Ala Gln Pro Ala Leu Thr Thr Thr Pro

500 505

<210> 6

<211> 509

<212> PRT

<213> Serratia sp. BW106

<400> 6

Met Ala Glu Asn Asn Ser Ala Ile His Ser Val Ala Val Ile Gly Ala

1 5 10 15

Gly Thr Met Gly Arg Gly Ile Ala Tyr Leu Leu Ala Gln Asn Gly Ile

20 25 30

Arg Thr Leu Leu Tyr Asn Arg Ser Gly Asn Asn Leu Asp Gln Ala Arg

35 40 45

Asp Tyr Ile Ile Arg Asp Leu Asp Lys Lys Ile Asp Asn Gly Lys Ile

50 55 60

Ser Gln Gln Lys Lys Gly Glu Val Leu Ala Asn Leu Val Phe Ser Pro

65 70 75 80

Ile Phe Asp Ala Ile Ala Asp Ser Asp Leu Val Ile Glu Thr Ile Ala

85 90 95

Glu His Glu Thr Thr Lys His Glu Ile Leu Ala Ala Ile Ala Ala Thr

100 105 110

Val Lys Gln Glu Ala Ile Ile Ala Thr Asn Thr Ser Ser Leu Ser Leu

115 120 125

Asn Lys Leu Ala Ala Gly Val Glu Asn Asn Ala Arg Phe Ile Gly Leu

130 135 140

His Phe Phe Asn Pro Ala Pro Leu Met Lys Leu Ile Glu Ile Ile Pro

145 150 155 160

Ser Tyr Phe Thr Ser Arg Ala Thr Ser Leu Arg Cys Gln Gln Leu Val

165 170 175

Thr Ala Leu Gly Lys Gln Phe Val Val Cys Lys Ala Thr Pro Gly Phe

180 185 190

Ile Val Asn Arg Met Ala Arg Pro Phe Tyr Leu Glu Gly Phe Arg Leu

195 200 205

Leu Glu Glu Asn Val Ala Leu Ala Pro Gln Ile Asp Arg Ala Leu Lys

210 215 220

Ala Gly Gly His Phe Arg Met Gly Pro Leu Glu Leu Thr Asp Phe Ile

225 230 235 240

Gly Gln Asp Ile Asn Tyr Gln Val Ser Ser Gln Ile Trp Gln Asp Met

245 250 255

Gln Tyr Asp Pro Arg Tyr Thr Pro Gly His Leu Gln Arg Ser Leu Val

260 265 270

Asp Ala Gly Leu Leu Gly Lys Lys Asn Gly Arg Ser Phe Phe Ala Ala

275 280 285

Pro Ser Ser Glu Ser Asn Pro Leu Asp Ala Gly Asn Gly Thr Leu Thr

290 295 300

Ser Leu His Phe Tyr Gly Glu His Thr Leu Phe Asp Leu Leu Gln Gln

305 310 315 320

Arg Ala Leu Ala Ile Trp Pro Thr Leu Gln Ile Ile His Gln Pro Glu

325 330 335

Arg Pro Thr Leu Gly Arg Phe Ile Arg Val Asn Asp Ala Leu Ala Val

340 345 350

Lys Ile Thr Asp Gly Arg Thr Ala Asn Leu Leu Ala Glu Leu Thr Asp

355 360 365

Leu Asp Thr Phe Val Ile Asp Ala Ala Leu Asn Tyr Ser Asp Thr Ala

370 375 380

Tyr Leu Val Ala Ala His Asn Gln Asp Ala Ala Glu Ala Asn Lys Ala

385 390 395 400

Leu Phe Leu Ser Leu Leu Gln Thr Leu Ile Pro Gln Val Glu Phe Ile

405 410 415

Lys Asp Ser Pro Gly Leu Ile Val Ala Arg Val Leu Ser Ser Leu Ile

420 425 430

Asn Glu Ser Val Ile Met Val Glu Ser Gly Val Cys Ser Arg Ala Asp

435 440 445

Ile Asp Ile Ala Ala Val Ala Gly Val Asn Tyr Ala Asp Gly Ile Phe

450 455 460

Ala Trp Leu Thr Gln Leu Gly Gln Lys Asn Val Lys Ser Thr Leu Asp

465 470 475 480

Asn Met Ala Gln Leu Leu His Ser Ala Arg Tyr Tyr Pro His Tyr Ser

485 490 495

Leu Leu Asn Ala Pro Arg Pro Glu Leu Ala Val Ala Pro

500 505

<210> 7

<211> 509

<212> PRT

<213> Serratia liquefaciens FK01

<400> 7

Met Ala Glu Asn Asn Thr Ala Ile Asp Ser Val Ala Val Ile Gly Ala

1 5 10 15

Gly Thr Met Gly Arg Gly Ile Ala Tyr Leu Leu Ala Leu Asn Gly Ile

20 25 30

Arg Thr Leu Leu Tyr Asn Arg Asn Gly Asn His Leu Asn Gln Ala Arg

35 40 45

Asp Tyr Ile Val Gly Asp Leu Asp Lys Lys Ile Asp Asn Gly Lys Ile

50 55 60

Ser Gln Gln Lys Lys Gly Glu Val Leu Ala Asn Leu Val Phe Ser Pro

65 70 75 80

Val Phe Asp Ala Ile Ala Asp Ser Asp Leu Val Ile Glu Thr Ile Ala

85 90 95

Glu His Glu Pro Thr Lys His Glu Ile Leu Ala Ala Ile Ala Ala Thr

100 105 110

Val Lys Lys Glu Ala Ile Ile Ala Thr Asn Thr Ser Ser Leu Ser Leu

115 120 125

Asn Lys Leu Ala Ala Gly Ile Glu Asn Asn Ser Arg Phe Ile Gly Leu

130 135 140

His Phe Phe Asn Pro Ala Pro Leu Met Lys Leu Ile Glu Ile Ile Pro

145 150 155 160

Ser Tyr Phe Thr Ala Arg Ala Thr Thr Leu Arg Cys Gln Gln Leu Val

165 170 175

Thr Ala Ile Gly Lys Lys Phe Val Val Cys Lys Ala Thr Pro Gly Phe

180 185 190

Ile Val Asn Arg Met Ala Arg Pro Phe Tyr Leu Glu Gly Phe Arg Leu

195 200 205

Leu Glu Glu Asn Val Ala Leu Ala Pro Gln Ile Asp Arg Ala Leu Lys

210 215 220

Ala Gly Gly His Phe Arg Met Gly Pro Leu Glu Leu Thr Asp Phe Ile

225 230 235 240

Gly Gln Asp Ile Asn Tyr Gln Val Ser Ser Gln Ile Trp Gln Asp Met

245 250 255

Gln Tyr Asp Pro Arg Tyr Thr Pro Gly His Leu Gln Arg Ser Leu Val

260 265 270

Asp Ala Gly Leu Leu Gly Lys Lys Asn Gly Arg Ser Phe Phe Ser Pro

275 280 285

Ser Ala Asp Ala Ala Asn Pro Pro Ala Thr Gly Gly Gly Thr Leu Ser

290 295 300

Ser Leu His Phe Phe Gly Glu His Pro Leu Phe Asp Leu Leu Gln Gln

305 310 315 320

His Ala Phe Ala Thr Trp Ala Pro Leu Ala Ile Thr Arg Gln Pro Glu

325 330 335

His Pro Val Leu Gly Arg Phe Ile Gln Val Asn Asp Ser Leu Ala Val

340 345 350

Lys Ile Thr Asp Gly Arg Thr Ala Asn Gln Leu Ala Glu Leu Ala Gly

355 360 365

Leu Asp Thr Phe Val Val Asp Val Ala Leu Asn Tyr Ala Asn Thr Ala

370 375 380

Phe Leu Val Ala Ala His Ser Gln Gln Ala Thr Glu Ala Asn Lys Glu

385 390 395 400

Leu Phe Leu Thr Leu Leu Gln Thr Val Ile Pro Gln Val Glu Phe Val

405 410 415

Lys Asp Ser Pro Gly Leu Ile Val Ala Arg Val Leu Ser Ser Leu Ile

420 425 430

Asn Glu Ser Val Ile Met Val Glu Ser Gly Val Cys Ser Arg Glu Asp

435 440 445

Ile Asp Ile Ala Ala Val Ala Gly Val Asn Tyr Ala Asp Gly Ile Phe

450 455 460

Ala Trp Leu Ala Gln Leu Gly Gln Lys Asn Val Lys Ser Thr Leu Asp

465 470 475 480

Asn Met Ala Gln Leu Leu His Ser Ala Arg Tyr Tyr Pro His Tyr Ser

485 490 495

Leu Leu His Ala Thr Arg Pro Glu Leu Ala Val Ala Pro

500 505

<210> 8

<211> 509

<212> PRT

<213> Serratia sp. S119

<400> 8

Met Ala Glu Ser Asn Ala Ala Ile Gln Ser Ala Ala Ile Ile Gly Ala

1 5 10 15

Gly Thr Met Gly Arg Gly Ile Ala Tyr Leu Phe Ala Gln Lys Gly Ile

20 25 30

Arg Thr Val Leu Tyr Asn Arg Asn Gly Asn Thr Leu Asn Gln Ala Arg

35 40 45

Glu Tyr Ile Ala Gln Asp Leu Asn Lys Lys Val Glu Gln Gly Lys Ile

50 55 60

Ala Leu Gln Asp Lys Gly Ala Val Leu Ala Asn Leu Val Phe Thr Ser

65 70 75 80

Val Phe Glu Thr Ile Ala Asp Ser Asp Leu Val Ile Glu Thr Ile Ala

85 90 95

Glu Gln Glu Gln Thr Lys Leu Glu Val Leu Ala Ala Ile Ala Ala Ala

100 105 110

Val Lys Pro Asp Thr Leu Ile Ala Thr Asn Thr Ser Ser Leu Ser Leu

115 120 125

Asn Lys Leu Ala Thr Ala Val Thr His Ser Glu Arg Phe Ile Gly Leu

130 135 140

His Phe Phe Asn Pro Ala Pro Leu Met Lys Leu Ile Glu Ile Ile Pro

145 150 155 160

Ala Tyr Phe Thr Ala Arg Ala Thr Thr Glu Arg Cys Arg Gln Leu Val

165 170 175

Ala Ala Leu Gly Lys Arg Asp Val Val Cys Gln Ala Thr Pro Gly Phe

180 185 190

Ile Val Asn Arg Met Ala Arg Pro Tyr Tyr Leu Glu Gly Phe Arg Leu

195 200 205

Leu Glu Glu His Val Ala Arg Ala Pro Gln Ile Asp Arg Ala Leu Lys

210 215 220

Ala Gly Gly His Phe Arg Met Gly Pro Leu Glu Leu Thr Asp Phe Ile

225 230 235 240

Gly Gln Asp Ile Asn Tyr Gln Val Ser Arg Gln Ile Trp Gln Asp Met

245 250 255

Gln Tyr Asp Pro Arg Tyr Thr Pro Gly His Leu Gln Arg Ser Leu Val

260 265 270

Asp Ala Gly Leu Leu Gly Lys Lys Asn Gly Arg Ser Tyr Phe Ala Ala

275 280 285

Glu Glu Asn Ala Leu Pro Val Thr Ala Ala Thr Asp Ala Asp Val Glu

290 295 300

Thr Leu Arg Val Tyr Gly Glu His Pro Phe Phe Thr Leu Leu Gln Gln

305 310 315 320

Arg Ala Ala Leu Gln Trp Pro Arg Leu Arg Val Glu Gln Arg Pro Ala

325 330 335

Leu Pro Gly Leu Gly Pro Ala Val Gln Ile Asn Glu Ala Phe Thr Val

340 345 350

Ser Val Thr Asp Gly Arg Thr Ala Ser Gln Leu Ala Glu Leu Thr Ala

355 360 365

Ala Asp Ala Phe Val Val Asp Val Ala Leu Asn Tyr Ala Asp Thr Ala

370 375 380

Tyr Leu Val Ala Ala His Asn Arg His Ala Ser Ala Ala Asn Lys Ala

385 390 395 400

Leu Phe Leu Arg Leu Leu His Thr Ala Ile Pro Gln Val Glu Phe Ile

405 410 415

Lys Asp Ser Pro Ala Leu Ile Val Ala Arg Val Leu Ser Ser Leu Ile

420 425 430

Asn Glu Ser Val Ile Met Val Glu Ser Gly Val Cys Ser Arg Glu Asp

435 440 445

Ile Asn Val Ala Ala Val Ala Gly Val Asn Tyr Ala Asp Gly Ile Phe

450 455 460

Gly Leu Leu Asp Ser Leu Gly Glu Lys Asn Val Arg Thr Thr Leu Ser

465 470 475 480

Asn Leu Ala Gln Leu Leu His Ala Ala Arg Tyr Ala Pro His Tyr Thr

485 490 495

Leu Leu His Ala Ala Gln Pro Ala Leu Thr Thr Thr Pro

500 505

<210> 9

<211> 509

<212> PRT

<213> Serratia sp. YD25

<400> 9

Met Ala Glu Arg Asn Ala Ala Ile Gln Ser Ala Ala Ile Ile Gly Ala

1 5 10 15

Gly Thr Met Gly Arg Gly Ile Ala Tyr Leu Phe Ala Gln Lys Gly Ile

20 25 30

Arg Thr Val Leu Tyr Asn Arg Asn Gly Asn Thr Leu Ile Gln Ala Arg

35 40 45

Glu Tyr Ile Val Gln Asp Leu Asp Lys Lys Val Glu Gln Gly Arg Leu

50 55 60

Ala Pro Gln Asp Lys Asp Ala Val Leu Ala Asn Leu Gln Phe Ser Ser

65 70 75 80

Val Phe Glu Ala Ile Val Asp Ser Asp Leu Val Leu Glu Thr Ile Ala

85 90 95

Glu Gln Glu Gln Ala Lys Leu Glu Val Leu Ala Ala Ile Ala Ala Ala

100 105 110

Val Lys Pro Asp Thr Leu Ile Ala Thr Asn Thr Ser Ser Leu Ser Leu

115 120 125

Asn Lys Leu Ala Thr Ala Val Thr His Ser Glu Arg Phe Ile Gly Leu

130 135 140

His Phe Phe Asn Pro Ala Pro Leu Met Lys Leu Ile Glu Ile Ile Pro

145 150 155 160

Ala Tyr Phe Thr Thr Gln Ala Thr Thr Glu Arg Cys Arg Gln Leu Val

165 170 175

Ala Ala Leu Gly Lys Arg Asp Val Val Cys Gln Ala Thr Pro Gly Phe

180 185 190

Ile Val Asn Arg Met Ala Arg Pro Tyr Tyr Leu Glu Gly Phe Arg Leu

195 200 205

Leu Glu Glu His Val Ala Arg Ala Pro Gln Ile Asp Arg Ala Leu Lys

210 215 220

Ala Gly Gly His Phe Arg Met Gly Pro Leu Glu Leu Thr Asp Phe Ile

225 230 235 240

Gly Gln Asp Ile Asn Tyr Gln Val Ser Arg Gln Ile Trp Gln Asp Met

245 250 255

Gln Tyr Asp Pro Arg Tyr Thr Pro Gly His Leu Gln Arg Ser Leu Val

260 265 270

Asp Ala Gly Leu Leu Gly Lys Lys Asn Gly Arg Ser Tyr Phe Ser Ala

275 280 285

Glu Glu Thr Ala Pro Pro Val Glu Ala Ala Ala Glu Ala Asp Val Glu

290 295 300

Thr Leu Arg Ile Tyr Gly Glu His Pro Leu Phe Thr Leu Leu Gln Gln

305 310 315 320

Arg Ala Ala Leu Gln Trp Pro Gln Leu Arg Val Glu Gln Arg Pro Ala

325 330 335

Leu Ser Gly Leu Gly Ala Ala Ile Gln Val Asn Asp Ala Phe Thr Val

340 345 350

Ser Val Thr Asp Gly Arg Thr Ala Asn Gln Leu Ala Glu Gln Thr Ala

355 360 365

Ala Asp Ala Phe Val Val Asp Val Ala Leu Asn Tyr Ala Asp Thr Ala

370 375 380

Tyr Leu Val Ala Ala His Ser Arg His Ala Ser Ala Ala Asn Lys Ala

385 390 395 400

Leu Phe Leu Arg Leu Leu His Thr Ala Leu Pro Gln Val Glu Phe Ile

405 410 415

Lys Asp Ser Pro Ala Leu Ile Val Ala Arg Val Leu Ser Ser Leu Ile

420 425 430

Asn Glu Ser Val Ile Met Val Glu Ser Gly Val Cys Ser Arg Glu Asp

435 440 445

Ile Asp Val Ala Ala Val Ala Gly Val Asn Tyr Ala Asp Gly Ile Phe

450 455 460

Gly Trp Leu Thr Arg Leu Gly Glu Lys Asn Val Arg Thr Thr Leu Ser

465 470 475 480

Asn Leu Ala Gln Leu Leu His Ala Ala Arg Tyr Ala Pro His Tyr Thr

485 490 495

Leu Leu His Ala Ala Gln Pro Ala Leu Thr Thr Thr Pro

500 505

<210> 10

<211> 509

<212> PRT

<213> Serratia sp. FS14

<400> 10

Met Ala Glu Ser Asn Ala Ala Ile Gln Ser Ala Ala Ile Ile Gly Ala

1 5 10 15

Gly Thr Met Gly Arg Gly Ile Ala Tyr Leu Phe Ala Gln Lys Gly Ile

20 25 30

Pro Thr Met Leu Tyr Asn Arg Asn Gly Asn Thr Leu Asn Gln Ala Arg

35 40 45

Glu Tyr Ile Ala Gln Asp Leu Asn Lys Lys Val Glu Gln Gly Lys Ile

50 55 60

Ala Leu Gln Asp Lys Asp Ala Val Leu Ala Asn Leu Met Phe Thr Ser

65 70 75 80

Val Phe Glu Ala Ile Ala Asp Ser Glu Leu Val Ile Glu Thr Ile Ala

85 90 95

Glu Gln Glu Gln Thr Lys Leu Glu Val Leu Ala Ala Ile Ala Ala Val

100 105 110

Val Lys Pro Asp Thr Leu Ile Ala Thr Asn Thr Ser Ser Leu Ser Leu

115 120 125

Asn Lys Leu Ala Thr Ala Val Thr His Ser Glu Arg Phe Ile Gly Leu

130 135 140

His Phe Phe Asn Pro Ala Pro Leu Met Lys Leu Ile Glu Ile Ile Pro

145 150 155 160

Ala Tyr Phe Thr Ala His Ala Thr Thr Glu Arg Cys Arg Gln Leu Val

165 170 175

Ala Ala Leu Gly Lys Arg Asp Val Val Cys Gln Ala Thr Pro Gly Phe

180 185 190

Ile Val Asn Arg Met Ala Arg Pro Tyr Tyr Leu Glu Gly Phe Arg Leu

195 200 205

Leu Glu Glu His Val Ala Arg Ala Ala Gln Ile Asp Arg Ala Leu Lys

210 215 220

Ala Gly Gly Arg Phe Arg Met Gly Pro Leu Glu Leu Thr Asp Phe Ile

225 230 235 240

Gly Gln Asp Ile Asn Tyr Gln Val Ser Arg Gln Ile Trp Gln Asp Met

245 250 255

Gln Tyr Asp Pro Arg Tyr Thr Pro Gly His Leu Gln Arg Ser Leu Val

260 265 270

Asp Ala Gly Leu Leu Gly Lys Lys Asn Gly Arg Ser Tyr Phe Ala Ala

275 280 285

Glu Glu Thr Ala Pro Pro Val Thr Ala Ala Asn Asn Ala Asp Val Glu

290 295 300

Thr Leu Arg Val Tyr Gly Glu His Pro Phe Phe Thr Leu Leu Gln Gln

305 310 315 320

Arg Ala Ala Leu Gln Trp Pro Gln Leu Arg Val Glu Gln Arg Pro Ala

325 330 335

Leu Pro Gly Leu Gly Ala Ala Val Gln Ile Asn Asp Ala Phe Thr Val

340 345 350

Ser Ile Thr Asp Gly Arg Thr Ala Ser Gln Leu Ala Glu Gln Thr Ala

355 360 365

Ala Asp Ala Phe Val Val Asp Val Ala Leu Asn Tyr Ala Asp Thr Thr

370 375 380

Tyr Leu Val Ala Ala His Ser Arg His Ala Ser Ala Ala Asn Lys Ala

385 390 395 400

Leu Phe Leu Arg Leu Leu His Thr Ala Ile Pro Gln Val Glu Phe Ile

405 410 415

Lys Asp Ser Pro Ala Leu Ile Val Ala Arg Val Leu Ser Ser Leu Ile

420 425 430

Asn Glu Ser Val Ile Met Val Glu Ser Gly Val Cys Ser Arg Glu Asp

435 440 445

Ile Asp Val Ala Ala Val Ala Gly Val Asn Tyr Ala Gly Gly Ile Phe

450 455 460

Asp Trp Leu Gly Lys Leu Gly Glu Lys Asn Val Arg Thr Thr Leu Ser

465 470 475 480

Asn Leu Ala Gln Leu Leu His Ala Ala Arg Tyr Ala Pro His Tyr Thr

485 490 495

Leu Leu His Ala Ala Gln Pro Ala Leu Thr Thr Thr Pro

500 505

<210> 11

<211> 509

<212> PRT

<213> Serratia sp. HMSC15F11

<400> 11

Met Ala Glu Ser Asn Ala Ala Ile Gln Ser Ala Ala Ile Ile Gly Ala

1 5 10 15

Gly Thr Met Gly Arg Gly Ile Ala Tyr Leu Phe Ala Gln Lys Gly Ile

20 25 30

Arg Thr Val Leu Tyr Asn Arg Asn Gly Asn Thr Leu Asn Gln Ala Arg

35 40 45

Asp Tyr Ile Glu Leu Asp Leu Asn Lys Lys Val Glu Gln Gly Lys Ile

50 55 60

Ala Leu Gln Asp Lys Gly Ala Val Leu Ala Asn Leu Val Phe Thr Ser

65 70 75 80

Val Phe Glu Thr Ile Ala Asp Ser Glu Leu Val Ile Glu Thr Ile Ala

85 90 95

Glu Gln Glu Gln Thr Lys Leu Glu Val Leu Ala Ala Ile Ala Ala Ala

100 105 110

Val Lys Pro Asp Thr Leu Ile Ala Thr Asn Thr Ser Ser Leu Ser Leu

115 120 125

Asn Lys Leu Ala Thr Ala Val Thr His Ser Glu Arg Phe Ile Gly Leu

130 135 140

His Phe Phe Asn Pro Ala Pro Leu Met Lys Leu Ile Glu Ile Ile Pro

145 150 155 160

Ala Tyr Phe Thr Ala His Ala Thr Thr Glu Arg Cys Arg Gln Leu Val

165 170 175

Ala Ala Leu Gly Lys Arg Asp Val Val Cys Gln Ala Thr Pro Gly Phe

180 185 190

Ile Val Asn Arg Met Ala Arg Pro Tyr Tyr Leu Glu Gly Phe Arg Leu

195 200 205

Leu Glu Glu His Val Ala Arg Ala Pro Gln Ile Asp Arg Ala Leu Lys

210 215 220

Ala Gly Gly His Phe Arg Met Gly Pro Leu Glu Leu Thr Asp Phe Ile

225 230 235 240

Gly Gln Asp Ile Asn Tyr Gln Val Ser Arg Gln Ile Trp Gln Asp Met

245 250 255

Gln Tyr Asp Ala Arg Tyr Thr Pro Gly His Leu Gln Arg Ser Leu Val

260 265 270

Asp Ala Gly Leu Leu Gly Lys Lys Asn Gly Arg Ser Tyr Phe Ala Val

275 280 285

Glu Glu Asn Ala Pro Pro Val Met Ala Ala Thr Asp Ala Asp Ile Glu

290 295 300

Thr Leu His Val Tyr Gly Glu His Pro Phe Phe Thr Leu Leu Gln Gln

305 310 315 320

Arg Ala Ala Leu Gln Trp Pro Gln Leu Arg Val Glu Gln Arg Pro Ala

325 330 335

Leu Pro Gly Leu Gly Pro Ala Val Arg Ile Asn Asp Ala Phe Thr Val

340 345 350

Ser Ile Thr Asp Gly Arg Thr Ala Asn Gln Leu Ala Glu Gln Thr Ala

355 360 365

Ala Asp Ala Phe Val Val Asp Val Ala Leu Asn Tyr Ala Asp Thr Ala

370 375 380

Tyr Leu Val Ala Ala His Ser Arg His Ala Ser Ala Ala Asn Lys Ala

385 390 395 400

Leu Phe Leu Arg Leu Leu His Thr Ala Ile Pro Gln Val Glu Phe Ile

405 410 415

Lys Asp Ser Pro Ala Leu Ile Val Ala Arg Val Leu Ser Ser Leu Ile

420 425 430

Asn Glu Ser Val Ile Met Val Glu Ser Gly Val Cys Ser Arg Glu Asp

435 440 445

Ile Asp Val Ala Ala Val Ala Gly Val Asn Tyr Ala Asp Gly Ile Phe

450 455 460

Gly Trp Leu Asp Ser Leu Gly Glu Lys Asn Val Arg Thr Thr Leu Ser

465 470 475 480

Asn Leu Ala Gln Leu Leu His Ala Ala Arg Tyr Ala Pro His Tyr Thr

485 490 495

Leu Leu His Ala Ala Gln Pro Ala Leu Thr Thr Thr Pro

500 505

<210> 12

<211> 509

<212> PRT

<213> Serratia sp. JKS000199

<400> 12

Met Ala Glu Ser Asn Ala Ala Ile Gln Ser Ala Ala Ile Ile Gly Ala

1 5 10 15

Gly Thr Met Gly Arg Gly Ile Ala Tyr Leu Leu Ala Gln Lys Ser Ile

20 25 30

Arg Thr Val Leu Tyr Asn Arg Asn Gly Asn Thr Leu Asn Gln Ala Arg

35 40 45

Asp Ala Ile Val Gln Asp Leu Asn Lys Lys Val Glu Gln Gly Lys Leu

50 55 60

Ala Leu Gln Asp Lys Asp Ala Val Leu Ala Asn Leu Thr Phe Ser Thr

65 70 75 80

Glu Phe Gly Ala Ile Ala Asp Ser Asp Leu Val Ile Glu Thr Ile Ala

85 90 95

Glu His Glu Gln Ala Lys Leu Glu Val Leu Ala Ala Ile Ala Ala Thr

100 105 110

Val Lys Pro Asp Thr Leu Ile Ala Thr Asn Thr Ser Ser Leu Ser Leu

115 120 125

Asn Lys Leu Ala Thr Ala Val Thr His Ser Glu Arg Phe Ile Gly Leu

130 135 140

His Phe Phe Asn Pro Ala Pro Leu Met Lys Leu Ile Glu Ile Ile Pro

145 150 155 160

Ala Tyr Phe Thr Ala Gln Val Thr Thr Glu Arg Cys Arg Gln Leu Val

165 170 175

Ala Ala Leu Gly Lys Arg Asp Val Val Cys Gln Ala Thr Pro Gly Phe

180 185 190

Ile Val Asn Arg Met Ala Arg Pro Tyr Tyr Leu Glu Gly Phe Arg Leu

195 200 205

Leu Glu Glu His Val Ala Arg Ala Pro Gln Ile Asp Arg Ala Leu Lys

210 215 220

Ala Gly Gly His Phe Arg Met Gly Pro Leu Glu Leu Thr Asp Phe Ile

225 230 235 240

Gly Gln Asp Ile Asn Tyr Gln Val Ser Arg Gln Ile Trp Gln Asp Met

245 250 255

Gln Tyr Asp Pro Arg Tyr Thr Pro Gly His Leu Gln Arg Ser Leu Val

260 265 270

Asp Ala Gly Leu Leu Gly Lys Lys Asn Gly Arg Ser Tyr Phe Ser Ala

275 280 285

Glu Glu Ser Pro Pro Pro Leu Ala Ala Ala Val Asp Ala Glu Val Glu

290 295 300

Thr Leu Arg Ile Tyr Gly Glu His Pro Leu Phe Thr Leu Leu Gln Gln

305 310 315 320

Arg Ala Ala Leu Gln Trp Pro Arg Leu Arg Val Glu Gln Arg Pro Thr

325 330 335

Leu Pro Gly Leu Gly Ala Ala Ile Gln Val Asn Asp Ala Phe Thr Val

340 345 350

Ser Val Thr Asp Gly Arg Thr Ala Asn Gln Leu Ala Glu Gln Thr Ala

355 360 365

Ala Asp Ala Phe Val Val Asp Val Ala Leu Asn Tyr Gly Asp Thr Ala

370 375 380

Tyr Leu Val Ala Ala His Ser Arg His Ala Ser Ala Ala Asn Lys Ala

385 390 395 400

Leu Phe Leu Arg Leu Leu His Thr Ala Ile Pro Leu Val Glu Phe Ile

405 410 415

Lys Asp Ser Pro Ala Leu Ile Val Ala Arg Val Leu Ser Ser Leu Ile

420 425 430

Asn Glu Ser Val Ile Met Val Glu Ser Gly Val Cys Ser Arg Glu Asp

435 440 445

Ile Asp Val Ala Ala Val Ala Gly Val Asn Tyr Ala Asp Gly Ile Phe

450 455 460

Gly Trp Leu Thr Arg Leu Gly Glu Glu Asn Val Arg Thr Thr Leu Ser

465 470 475 480

Asn Leu Ala Gln Leu Leu His Ala Ala Arg Tyr Ala Pro His Tyr Thr

485 490 495

Leu Leu His Ala Ala Gln Pro Ala Leu Thr Thr Thr Pro

500 505

<210> 13

<211> 509

<212> PRT

<213> Serratia sp. TEL

<400> 13

Met Ala Glu Ser Asn Ala Ala Ile Gln Ser Ala Ala Ile Ile Gly Ala

1 5 10 15

Gly Thr Met Gly Arg Gly Ile Ala Tyr Leu Leu Ala Gln Lys Ser Ile

20 25 30

Arg Thr Val Leu Tyr Asn Arg Asn Gly Asn Thr Leu Asn Gln Ala Arg

35 40 45

Asp Ala Ile Val Gln Asp Leu Asn Lys Lys Val Glu Gln Gly Lys Leu

50 55 60

Ala Leu Gln Asp Lys Asp Ala Val Leu Ala Asn Leu Thr Phe Ser Ala

65 70 75 80

Glu Phe Gly Ala Ile Ala Asp Ser Asp Leu Val Ile Glu Thr Ile Ala

85 90 95

Glu His Glu Gln Ala Lys Leu Glu Val Leu Ala Ala Ile Ala Ala Thr

100 105 110

Val Lys Pro Asp Thr Leu Ile Ala Thr Asn Thr Ser Ser Leu Ser Leu

115 120 125

Asn Lys Leu Ala Thr Ala Val Thr His Ser Glu Arg Phe Ile Gly Leu

130 135 140

His Phe Phe Asn Pro Ala Pro Leu Met Lys Leu Ile Glu Ile Ile Pro

145 150 155 160

Ala Tyr Phe Thr Ala Gln Val Thr Thr Glu Arg Cys Arg Gln Leu Val

165 170 175

Ala Ala Leu Gly Lys Arg Asp Val Val Cys Gln Ala Thr Pro Gly Phe

180 185 190

Ile Val Asn Arg Met Ala Arg Pro Tyr Tyr Leu Glu Gly Phe Arg Leu

195 200 205

Leu Glu Glu His Val Ala Arg Ala Pro Gln Ile Asp Arg Ala Leu Lys

210 215 220

Ala Gly Gly His Phe Arg Met Gly Pro Leu Glu Leu Thr Asp Phe Ile

225 230 235 240

Gly Gln Asp Ile Asn Tyr Gln Val Ser Arg Gln Ile Trp Gln Asp Met

245 250 255

Gln Tyr Asp Pro Arg Tyr Thr Pro Gly His Leu Gln Arg Ser Leu Val

260 265 270

Asp Ala Gly Leu Leu Gly Lys Lys Asn Gly Arg Ser Tyr Phe Ser Ala

275 280 285

Glu Glu Ser Pro Pro Pro Leu Ala Ala Ala Val Asp Ala Glu Val Glu

290 295 300

Thr Leu Arg Ile Tyr Gly Glu His Pro Leu Phe Thr Leu Leu Gln Gln

305 310 315 320

Arg Ala Thr Leu Gln Trp Pro Arg Leu Arg Val Glu Gln Arg Pro Thr

325 330 335

Leu Pro Gly Leu Gly Ala Ala Ile Gln Val Asn Asp Ala Phe Thr Val

340 345 350

Ser Val Thr Asp Gly Arg Thr Ala Asn Gln Leu Ala Glu Gln Thr Ala

355 360 365

Ala Asp Ala Phe Val Val Asp Val Ala Leu Asn Tyr Gly Asp Thr Ala

370 375 380

Tyr Leu Val Ala Ala His Ser Arg His Ala Ser Ala Ala Asn Lys Ala

385 390 395 400

Leu Phe Leu Arg Leu Leu His Thr Ala Ile Pro Gln Val Glu Phe Ile

405 410 415

Lys Asp Ser Pro Ala Leu Ile Val Ala Arg Val Leu Ser Ser Leu Ile

420 425 430

Asn Glu Ser Val Ile Met Val Glu Ser Gly Val Cys Ser Arg Glu Asp

435 440 445

Ile Asp Val Ala Ala Val Ala Gly Val Asn Tyr Ala Asp Gly Ile Phe

450 455 460

Gly Trp Leu Thr Arg Leu Gly Glu Glu Asn Val Arg Ala Thr Leu Ser

465 470 475 480

Asn Leu Ala Gln Leu Leu His Ala Ala Arg Tyr Ala Pro His Tyr Thr

485 490 495

Leu Leu His Ala Ala Gln Pro Ala Leu Thr Thr Thr Pro

500 505

<210> 14

<211> 509

<212> PRT

<213> Serratia sp. ISTD04

<400> 14

Met Ala Glu Ser Asn Ala Ala Ile Gln Ser Ala Ala Ile Ile Gly Ala

1 5 10 15

Gly Thr Met Gly Arg Gly Ile Ala Tyr Leu Leu Ala Gln Lys Ser Ile

20 25 30

Arg Thr Val Leu Tyr Asn Arg Asn Gly Asn Thr Leu Asn Gln Ala Arg

35 40 45

Asp Ala Ile Val Gln Asp Leu Asn Lys Lys Val Glu Gln Gly Lys Leu

50 55 60

Ala Leu Gln Asp Lys Asp Ala Val Leu Ala Asn Leu Thr Phe Ser Ala

65 70 75 80

Glu Phe Gly Val Ile Ala Asp Ser Asp Leu Val Ile Glu Thr Ile Ala

85 90 95

Glu His Glu Gln Ala Lys Leu Glu Val Leu Ala Ala Ile Ala Ala Thr

100 105 110

Val Lys Ser Asp Thr Leu Ile Ala Thr Asn Thr Ser Ser Leu Ser Leu

115 120 125

Asn Lys Leu Ala Thr Ala Val Thr His Ser Glu Arg Phe Ile Gly Leu

130 135 140

His Phe Phe Asn Pro Ala Pro Leu Met Lys Leu Ile Glu Ile Ile Pro

145 150 155 160

Ala Tyr Phe Thr Ala Gln Val Thr Thr Glu Arg Cys Arg Gln Leu Val

165 170 175

Ala Ala Leu Gly Lys Arg Asp Val Val Cys Gln Ala Thr Pro Gly Phe

180 185 190

Ile Val Asn Arg Met Ala Arg Pro Tyr Tyr Leu Glu Gly Phe Arg Leu

195 200 205

Leu Glu Glu His Val Ala Arg Ala Pro Gln Ile Asp Arg Ala Leu Lys

210 215 220

Ala Gly Gly His Phe Arg Met Gly Pro Leu Glu Leu Thr Asp Phe Ile

225 230 235 240

Gly Gln Asp Ile Asn Tyr Gln Val Ser Arg Gln Ile Trp Gln Asp Met

245 250 255

Gln Tyr Asp Pro Arg Tyr Thr Pro Gly His Leu Gln Arg Ser Leu Val

260 265 270

Asp Ala Gly Leu Leu Gly Lys Lys Asn Gly Arg Ser Tyr Phe Ser Ala

275 280 285

Glu Glu Ser Pro Pro Pro Leu Ala Ala Ala Val Asp Ala Glu Val Glu

290 295 300

Thr Leu Arg Ile Tyr Gly Glu His Pro Leu Phe Thr Leu Leu Gln Gln

305 310 315 320

Arg Ala Ala Leu Gln Trp Pro Arg Leu Arg Val Glu Gln Arg Pro Thr

325 330 335

Leu Pro Gly Leu Gly Ala Ala Ile Gln Val Asn Asp Ala Phe Thr Val

340 345 350

Ser Val Thr Asp Gly Arg Thr Ala Asn Gln Leu Ala Glu Gln Thr Ala

355 360 365

Ala Asp Ala Phe Val Val Asp Val Ala Leu Asn Tyr Gly Asp Thr Ala

370 375 380

Tyr Leu Val Ala Ala His Ser Arg His Ala Ser Ala Ala Asn Lys Ala

385 390 395 400

Leu Phe Leu Arg Leu Leu His Thr Ala Ile Pro Gln Val Glu Phe Ile

405 410 415

Lys Asp Ser Pro Ala Leu Ile Val Ala Arg Val Leu Ser Ser Leu Ile

420 425 430

Asn Glu Ser Val Ile Met Val Glu Ser Gly Val Cys Ser Arg Glu Asp

435 440 445

Ile Asp Val Ala Ala Val Ala Gly Val Asn Tyr Ala Asp Gly Ile Phe

450 455 460

Gly Trp Leu Thr Arg Leu Gly Glu Glu Asn Val Arg Thr Thr Leu Ser

465 470 475 480

Asn Leu Ala Gln Leu Leu His Ala Ala Arg Tyr Ala Pro His Tyr Thr

485 490 495

Leu Leu His Ala Ala Gln Pro Ala Leu Thr Thr Thr Pro

500 505

<210> 15

<211> 509

<212> PRT

<213> Serratia sp. SCBI

<400> 15

Met Ala Glu Ser Asn Ala Ala Ile Gln Ser Ala Ala Ile Ile Gly Ala

1 5 10 15

Gly Thr Met Gly Arg Gly Ile Ala Tyr Leu Leu Ala Gln Lys Ser Ile

20 25 30

Arg Thr Val Leu Tyr Asn Arg Asn Gly Asn Thr Leu Asn Gln Ala Arg

35 40 45

Asp Ala Ile Val Gln Asp Leu Asn Lys Lys Val Glu Gln Gly Lys Leu

50 55 60

Ala Leu Gln Asp Lys Asp Ala Val Leu Ala Asn Leu Thr Phe Ser Ala

65 70 75 80

Glu Phe Gly Ala Ile Ala Asp Ser Asp Leu Val Ile Glu Thr Ile Ala

85 90 95

Glu His Glu Gln Ala Lys Leu Glu Val Leu Ala Ala Ile Ala Ala Thr

100 105 110

Val Lys Pro Asp Thr Leu Ile Ala Thr Asn Thr Ser Ser Leu Ser Leu

115 120 125

Asn Lys Leu Ala Thr Ala Val Thr His Ser Glu Arg Phe Ile Gly Leu

130 135 140

His Phe Phe Asn Pro Ala Pro Leu Met Lys Leu Ile Glu Ile Ile Pro

145 150 155 160

Ala Tyr Phe Thr Ala Gln Val Thr Thr Glu Arg Cys Arg Gln Leu Val

165 170 175

Ala Ala Leu Gly Lys Arg Asp Val Val Cys Gln Ala Thr Pro Gly Phe

180 185 190

Ile Val Asn Arg Met Ala Arg Pro Tyr Tyr Leu Glu Gly Phe Arg Leu

195 200 205

Leu Glu Glu His Val Ala Arg Ala Pro Gln Ile Asp Arg Ala Leu Lys

210 215 220

Ala Gly Gly His Phe Arg Met Gly Pro Leu Glu Leu Thr Asp Phe Ile

225 230 235 240

Gly Gln Asp Ile Asn Tyr Gln Val Ser Arg Gln Ile Trp Gln Asp Met

245 250 255

Gln Tyr Asp Pro Arg Tyr Thr Pro Gly His Leu Gln Arg Ser Leu Val

260 265 270

Asp Ala Gly Leu Leu Gly Lys Lys Asn Gly Arg Ser Tyr Phe Ser Thr

275 280 285

Glu Glu Ser Pro Pro Pro Leu Ala Ala Ala Val Asp Ala Glu Val Glu

290 295 300

Thr Leu Arg Ile Tyr Gly Glu His Pro Leu Phe Thr Leu Leu Gln Gln

305 310 315 320

Arg Ala Ala Leu Gln Trp Pro Arg Leu Arg Val Glu Gln Arg Pro Thr

325 330 335

Leu Pro Gly Leu Gly Ala Ala Ile Gln Val Asn Asp Ala Phe Thr Val

340 345 350

Ser Val Thr Asp Gly Arg Thr Ala Asn Gln Leu Ala Glu Gln Thr Ala

355 360 365

Ala Asp Ala Phe Val Val Asp Val Ala Leu Asn Tyr Gly Asp Thr Ala

370 375 380

Tyr Leu Val Ala Ala His Ser Arg His Ala Ser Ala Ala Asn Lys Ala

385 390 395 400

Leu Phe Leu Arg Leu Leu His Thr Ala Ile Pro Gln Val Glu Phe Ile

405 410 415

Lys Asp Ser Pro Ala Leu Ile Val Ala Arg Val Leu Ser Ser Leu Ile

420 425 430

Asn Glu Ser Val Ile Met Val Glu Ser Gly Val Cys Ser Arg Glu Asp

435 440 445

Ile Asp Val Ala Ala Val Ala Gly Val Asn Tyr Ala Asp Gly Ile Phe

450 455 460

Gly Trp Leu Thr Arg Leu Gly Glu Glu Asn Val Arg Thr Thr Leu Ser

465 470 475 480

Asn Leu Ala Gln Leu Leu His Ala Ala Arg Tyr Ala Pro His Tyr Thr

485 490 495

Leu Leu His Ala Ala Gln Pro Ala Leu Thr Thr Thr Pro

500 505

<210> 16

<211> 509

<212> PRT

<213> Serratia sp. S4

<400> 16

Met Ala Glu Asn Asn Ser Ala Ile His Ser Val Ala Val Ile Gly Ala

1 5 10 15

Gly Thr Met Gly Arg Gly Ile Ala Tyr Leu Leu Ala Gln Asn Gly Ile

20 25 30

Arg Thr Leu Leu Tyr Asn Arg Ser Gly Asn Asn Leu Asn Gln Ala Arg

35 40 45

Asp Tyr Ile Ile Arg Asp Leu Asp Lys Lys Ile Asp Gly Gly Lys Ile

50 55 60

Ser Leu Gln Lys Lys Gly Glu Ile Leu Ala Asn Leu Val Phe Ser Pro

65 70 75 80

Ile Phe Glu Ala Ile Ala Asp Ser Asp Leu Val Ile Glu Thr Ile Ala

85 90 95

Glu His Glu Ser Thr Lys His Glu Ile Leu Ser Ala Ile Ala Ala Thr

100 105 110

Val Lys Lys Glu Ala Ile Ile Ala Thr Asn Thr Ser Ser Leu Ser Leu

115 120 125

Asn Lys Leu Ala Ala Gly Val Glu Asn Asn Ala Arg Phe Ile Gly Leu

130 135 140

His Phe Phe Asn Pro Ala Pro Leu Met Lys Leu Ile Glu Ile Ile Pro

145 150 155 160

Ser Tyr Phe Thr Ser Arg Ala Thr Ser Leu Arg Cys Gln Gln Leu Val

165 170 175

Thr Val Ile Gly Lys Gln Phe Val Val Cys Lys Ala Thr Pro Gly Phe

180 185 190

Ile Val Asn Arg Met Ala Arg Pro Phe Tyr Leu Glu Gly Phe Arg Leu

195 200 205

Leu Glu Glu Asn Val Ala Leu Ala Pro Gln Ile Asp Arg Ala Leu Lys

210 215 220

Ala Gly Gly His Phe Arg Met Gly Pro Leu Glu Leu Thr Asp Phe Ile

225 230 235 240

Gly Gln Asp Ile Asn Tyr Gln Val Ser Ser Gln Ile Trp Gln Asp Met

245 250 255

Gln Tyr Asp Pro Arg Tyr Thr Pro Gly His Leu Gln Arg Ser Leu Val

260 265 270

Asp Ala Gly Leu Leu Gly Lys Lys Asn Gly Arg Ser Phe Phe Thr Pro

275 280 285

Ser Ser Ala Thr Pro Ser Ser Ala Asp Ala Gly Ser Gly Thr Pro Thr

290 295 300

Ser Leu Asn Phe Tyr Gly Glu His Pro Leu Phe Asp Leu Leu Gln Gln

305 310 315 320

Arg Ala Leu Ala Leu Trp Pro Arg Leu Gln Ile Asn Arg Gln Ser Glu

325 330 335

Gln Pro Thr Leu Gly Arg Phe Ile Arg Val Asn Asp Ala Met Ala Ile

340 345 350

Lys Ile Thr Asp Gly Arg Thr Ala Asn Leu Leu Ala Glu Leu Thr Glu

355 360 365

Leu Asp Thr Phe Val Ile Asp Ala Ala Leu Asn Tyr Ala Asp Thr Ala

370 375 380

Tyr Leu Ala Ala Ala His Ser Gln Asp Ala Ser Thr Ala Asn Lys Ala

385 390 395 400

Leu Phe Leu Thr Leu Leu Gln Thr Leu Ile Pro Gln Val Glu Phe Ile

405 410 415

Lys Asp Ser Pro Gly Leu Ile Val Ala Arg Val Leu Ser Ser Leu Ile

420 425 430

Asn Glu Ser Val Ile Met Val Glu Ser Gly Val Cys Ser Arg Ala Asp

435 440 445

Ile Asp Ile Ala Ala Val Ala Gly Val Asn Tyr Ala Asp Gly Ile Phe

450 455 460

Ser Trp Leu Ala Gln Leu Gly Gln Lys Asn Val Lys Ser Thr Leu Asp

465 470 475 480

Asn Met Ala Gln Leu Leu His Ser Ala Arg Tyr Tyr Pro His Tyr Ser

485 490 495

Leu Leu Asn Thr Pro Arg Pro Glu Leu Ala Val Ala Pro

500 505

<210> 17

<211> 509

<212> PRT

<213> Serratia sp. C-1

<400> 17

Met Ala Glu Asn Asn Ser Ala Ile Arg Ser Ala Ala Val Ile Gly Ala

1 5 10 15

Gly Thr Met Gly Arg Gly Ile Ala Tyr Leu Leu Ala Leu Asn Gly Ile

20 25 30

Arg Thr Val Leu Tyr Asn Arg Asn Gly Asn Asn Leu Asn Gln Ala Arg

35 40 45

Asp Tyr Ile Val Ser Asp Leu Asp Arg Lys Ile Asp Asn Gly Lys Ile

50 55 60

Thr Leu Gln Lys Lys Gly Gln Ile Leu Ala Asn Ile Ile Phe Ser Ala

65 70 75 80

Val Phe Asp Ala Ile Thr Asp Ser Asp Leu Val Ile Glu Thr Ile Ala

85 90 95

Glu Asp Glu Gln Thr Lys His Glu Ile Leu Ala Ala Ile Ala Ala Thr

100 105 110

Val Lys Pro Glu Ala Ile Ile Ala Thr Asn Thr Ser Ser Leu Ser Leu

115 120 125

Asn Lys Leu Ala Ala Gly Val Glu Asn Asn Pro Arg Phe Ile Gly Leu

130 135 140

His Phe Phe Asn Pro Ala Pro Leu Met Lys Leu Ile Glu Ile Ile Pro

145 150 155 160

Ser Tyr Phe Thr Ser His Ala Thr Ser Leu Arg Cys Gln Lys Leu Val

165 170 175

Ile Ala Leu Gly Lys Gln Phe Val Val Cys Lys Ala Thr Pro Gly Phe

180 185 190

Ile Val Asn Arg Met Ala Arg Pro Phe Tyr Leu Glu Gly Phe Arg Leu

195 200 205

Leu Glu Glu Asn Val Ala Leu Ala Pro Gln Ile Asp Arg Ala Leu Lys

210 215 220

Ala Gly Gly His Phe Arg Met Gly Pro Leu Glu Leu Thr Asp Phe Ile

225 230 235 240

Gly Gln Asp Ile Asn Tyr Gln Val Ser Lys Gln Ile Trp Gln Asp Met

245 250 255

Gln Phe Asp Pro Arg Tyr Thr Pro Gly His Leu Gln Arg Ser Leu Val

260 265 270

Asp Ala Gly Leu Leu Gly Arg Lys Asn Gly Arg Ser Phe Phe Ala Ser

275 280 285

Gln Pro Ala Thr Pro Pro Thr Pro Thr Thr Glu Ser Asp Thr Pro Thr

290 295 300

Ser Leu His Phe Tyr Gly Glu His Ala Leu Phe Asp His Leu Gln Gln

305 310 315 320

Arg Ala Leu Ala Ala Trp Pro Ala Leu Arg Val Gln Arg Leu Pro Glu

325 330 335

Arg Pro Glu Leu Gly Arg Phe Ile Leu Val Asn Asn Ala Leu Ala Ile

340 345 350

Lys Ile Thr Asp Gly Arg Thr Ala Asn Leu Leu Ala Gly Leu Thr Ala

355 360 365

Leu Asp Thr Phe Val Ile Asp Ala Ala Leu Asn Tyr Ala Asp Thr Ala

370 375 380

Tyr Leu Val Ala Ala His Asn Gln His Ala Thr Glu Thr Asn Lys Ala

385 390 395 400

Leu Phe Leu Thr Leu Leu Gln Thr Val Ile Ala Gln Val Glu Phe Ile

405 410 415

Lys Asp Ser Pro Ala Leu Ile Val Ala Arg Val Leu Ser Ser Leu Ile

420 425 430

Asn Glu Ser Val Ile Met Val Glu Ser Gly Val Cys Ser Arg Ala Asp

435 440 445

Ile Asp Ile Ala Ala Val Ala Gly Val Asn Tyr Ala Asp Gly Ile Phe

450 455 460

Ala Trp Leu Ala Gln Leu Gly Gln Lys Asn Val Lys Ser Thr Leu Asp

465 470 475 480

Asn Met Ala Gln Leu Leu His Ser Ala Arg Tyr Tyr Pro His Tyr Ser

485 490 495

Leu Leu Asn Ala Ala Arg Pro Glu Leu Ala Val Ala Pro

500 505

<210> 18

<211> 509

<212> PRT

<213> Serratia marcescens 532

<400> 18

Met Ala Glu Ser Asn Ala Ala Ile Gln Ser Ala Ala Ile Ile Gly Ala

1 5 10 15

Gly Thr Met Gly Arg Gly Ile Ala Tyr Leu Phe Ala Gln Lys Gly Ile

20 25 30

Arg Thr Val Leu Tyr Asn Arg Asn Gly Asn Thr Leu Asn Gln Ala Arg

35 40 45

Glu Tyr Ile Ala Gln Asp Leu Asn Lys Lys Val Glu Gln Gly Lys Ile

50 55 60

Ala Leu Gln Asp Lys Gly Ala Val Leu Ala Asn Leu Met Phe Thr Ser

65 70 75 80

Val Phe Glu Ala Ile Thr Asp Ser Glu Leu Val Ile Glu Thr Ile Ala

85 90 95

Glu Gln Glu Gln Thr Lys Leu Glu Val Leu Ala Ala Ile Ala Ala Val

100 105 110

Val Lys Pro Asp Thr Leu Ile Ala Thr Asn Thr Ser Ser Leu Ser Leu

115 120 125

Asn Lys Leu Ala Thr Ala Val Thr His Ser Glu Arg Phe Ile Gly Leu

130 135 140

His Phe Phe Asn Pro Ala Pro Leu Met Lys Leu Ile Glu Ile Ile Pro

145 150 155 160

Ala Tyr Phe Thr Ala His Ala Thr Thr Glu Arg Cys Arg Gln Leu Val

165 170 175

Ala Ala Leu Gly Lys His Asp Val Val Cys Gln Ala Thr Pro Gly Phe

180 185 190

Ile Val Asn Arg Met Ala Arg Pro Tyr Tyr Leu Glu Gly Phe Arg Leu

195 200 205

Leu Glu Glu His Val Ala Arg Ala Ala Gln Ile Asp Arg Ala Leu Lys

210 215 220

Ala Gly Gly Arg Phe Arg Met Gly Pro Leu Glu Leu Thr Asp Phe Ile

225 230 235 240

Gly Gln Asp Ile Asn Tyr Gln Val Ser Arg Gln Ile Trp Gln Asp Met

245 250 255

Gln Tyr Asp Pro Arg Tyr Thr Pro Gly His Leu Gln Arg Ser Leu Val

260 265 270

Asp Ala Gly Leu Leu Gly Lys Lys Asn Gly Arg Ser Tyr Phe Ala Ala

275 280 285

Glu Glu Thr Ala Pro Pro Val Thr Ala Ala Ser Asn Ala Asp Val Glu

290 295 300

Thr Leu Arg Val Tyr Gly Glu His Pro Phe Phe Thr Leu Leu Gln Gln

305 310 315 320

Arg Ala Ala Leu Gln Trp Pro Gln Leu Arg Val Glu Gln Arg Pro Ala

325 330 335

Leu Pro Gly Leu Gly Ser Ala Val Gln Ile Asn Asp Ala Phe Thr Val

340 345 350

Ser Ile Thr Asp Gly Arg Thr Ala Ser Gln Leu Ala Glu Gln Thr Ala

355 360 365

Ala Asp Ala Phe Val Val Asp Val Ala Leu Asn Tyr Ala Asp Thr Thr

370 375 380

Tyr Leu Ala Ala Ala His Ser Arg His Ala Ser Ala Ala Asn Lys Ala

385 390 395 400

Leu Phe Leu Arg Leu Leu His Thr Ala Ile Pro Gln Val Glu Phe Ile

405 410 415

Lys Asp Ser Pro Ala Leu Ile Val Ala Arg Val Leu Ser Ser Leu Ile

420 425 430

Asn Glu Ser Val Ile Met Val Glu Ser Gly Val Cys Ser Arg Glu Asp

435 440 445

Ile Asp Val Ala Ala Val Ala Gly Val Asn Tyr Ala Gly Gly Ile Phe

450 455 460

Asp Trp Leu Gly Lys Leu Gly Glu Lys Asn Val Arg Thr Thr Leu Ser

465 470 475 480

Asn Leu Ala Gln Leu Leu His Ala Ala Arg Tyr Ala Pro His Tyr Thr

485 490 495

Leu Leu His Ala Ala Gln Pro Ala Leu Thr Thr Thr Pro

500 505

<210> 19

<211> 509

<212> PRT

<213> Serratia marcescens 2880STDY5683033

<400> 19

Met Ala Glu Ser Asn Ala Ala Ile Gln Ser Ala Ala Ile Ile Gly Ala

1 5 10 15

Gly Thr Met Gly Arg Gly Ile Ala Tyr Leu Phe Ala Gln Lys Gly Ile

20 25 30

Arg Thr Val Leu Tyr Asn Arg Asn Gly Asn Thr Leu Asn Gln Ala Arg

35 40 45

Glu Tyr Ile Ala His Asp Leu Asn Lys Lys Val Glu Gln Gly Lys Ile

50 55 60

Ala Leu Gln Asp Lys Gly Ala Val Leu Ala Asn Leu Met Phe Thr Ser

65 70 75 80

Val Phe Glu Ala Ile Ala Asp Ser Glu Leu Val Ile Glu Thr Ile Ala

85 90 95

Glu Gln Glu Gln Thr Lys Leu Glu Val Leu Ala Ala Ile Ala Ala Val

100 105 110

Val Lys Pro Asp Thr Leu Ile Ala Thr Asn Thr Ser Ser Leu Ser Leu

115 120 125

Asn Lys Leu Ala Thr Ala Val Thr His Ser Glu Arg Phe Ile Gly Leu

130 135 140

His Phe Phe Asn Pro Ala Pro Leu Met Lys Leu Ile Glu Ile Ile Pro

145 150 155 160

Ala Tyr Phe Thr Ala His Ala Thr Thr Glu Arg Cys Arg Gln Leu Val

165 170 175

Ala Ala Leu Gly Lys His Asp Val Val Cys Gln Ala Thr Pro Gly Phe

180 185 190

Ile Val Asn Arg Met Ala Arg Pro Tyr Tyr Leu Glu Gly Phe Arg Leu

195 200 205

Leu Glu Glu His Val Ala Arg Ala Ala Gln Ile Asp Arg Ala Leu Lys

210 215 220

Ala Gly Gly Arg Phe Arg Met Gly Pro Leu Glu Leu Thr Asp Phe Ile

225 230 235 240

Gly Gln Asp Ile Asn Tyr Gln Val Ser Arg Gln Ile Trp Gln Asp Met

245 250 255

Gln Tyr Asp Pro Arg Tyr Thr Pro Gly His Leu Gln Arg Ser Leu Val

260 265 270

Asp Ala Gly Leu Leu Gly Lys Lys Asn Gly Arg Ser Tyr Phe Ala Thr

275 280 285

Glu Glu Thr Ala Pro Pro Val Thr Ala Ala Ser Asn Ala Asp Val Glu

290 295 300

Thr Leu Arg Val Tyr Gly Glu His Pro Phe Phe Thr Leu Leu Gln Gln

305 310 315 320

Arg Ala Ala Leu Gln Trp Pro Gln Leu Arg Val Glu Gln Arg Pro Ala

325 330 335

Leu Pro Gly Leu Gly Ser Ala Val Gln Ile Asn Asp Ala Phe Thr Val

340 345 350

Ser Ile Thr Asp Gly Arg Thr Ala Ser Gln Leu Ala Glu Gln Thr Ala

355 360 365

Ala Asp Ala Phe Val Val Asp Val Ala Leu Asn Tyr Ala Asp Thr Thr

370 375 380

Tyr Leu Ala Ala Ala His Ser Arg His Ala Ser Ala Ala Asn Lys Ala

385 390 395 400

Leu Phe Leu Arg Leu Leu His Thr Ala Ile Pro Gln Val Glu Phe Ile

405 410 415

Lys Asp Ser Pro Ala Leu Ile Val Ala Arg Val Leu Ser Ser Leu Ile

420 425 430

Asn Glu Ser Val Ile Met Val Glu Ser Gly Val Cys Ser Arg Glu Asp

435 440 445

Ile Asp Val Ala Ala Val Ala Gly Val Asn Tyr Ala Gly Gly Ile Phe

450 455 460

Asp Trp Leu Gly Lys Leu Gly Glu Lys Asn Val Arg Thr Thr Leu Ser

465 470 475 480

Asn Leu Ala Gln Leu Leu His Ala Ala Arg Tyr Ala Pro His Tyr Thr

485 490 495

Leu Leu His Ala Ala Gln Pro Ala Leu Thr Thr Thr Pro

500 505

<210> 20

<211> 509

<212> PRT

<213> Serratia marcescens WW4

<400> 20

Met Ala Glu Ser Asn Ala Ala Ile Gln Ser Ala Ala Ile Ile Gly Ala

1 5 10 15

Gly Thr Met Gly Arg Gly Ile Ala Tyr Leu Phe Ala Gln Lys Gly Ile

20 25 30

Pro Thr Met Leu Tyr Asn Arg Asn Gly Asn Thr Leu Asn Gln Ala Arg

35 40 45

Glu Tyr Ile Ala Gln Asp Leu Asn Lys Lys Val Glu Gln Gly Lys Ile

50 55 60

Ala Leu Gln Asp Lys Asp Ala Val Leu Ala Asn Leu Met Phe Thr Ser

65 70 75 80

Val Phe Glu Ala Ile Ala Asp Ser Glu Leu Val Ile Glu Thr Ile Ala

85 90 95

Glu Gln Glu Gln Thr Lys Leu Glu Val Leu Ala Ala Ile Ala Ala Val

100 105 110

Val Lys Pro Asp Thr Leu Ile Ala Thr Asn Thr Ser Ser Leu Ser Leu

115 120 125

Asn Lys Leu Ala Thr Ala Val Thr His Ser Glu Arg Phe Ile Gly Leu

130 135 140

His Phe Phe Asn Pro Ala Pro Leu Met Lys Leu Ile Glu Ile Ile Pro

145 150 155 160

Ala Tyr Phe Thr Ala His Ala Thr Thr Glu Arg Cys Arg Gln Leu Val

165 170 175

Ala Ala Leu Gly Lys Arg Asp Val Val Cys Gln Ala Thr Pro Gly Phe

180 185 190

Ile Val Asn Arg Met Ala Arg Pro Tyr Tyr Leu Glu Gly Phe Arg Leu

195 200 205

Leu Glu Glu His Val Ala Arg Ala Ala Gln Ile Asp Arg Ala Leu Lys

210 215 220

Ala Gly Gly Arg Phe Arg Met Gly Pro Leu Glu Leu Thr Asp Phe Ile

225 230 235 240

Gly Gln Asp Ile Asn Tyr Gln Val Ser Arg Gln Ile Trp Gln Asp Met

245 250 255

Gln Tyr Asp Pro Arg Tyr Thr Pro Gly His Leu Gln Arg Ser Leu Val

260 265 270

Asp Ala Gly Leu Leu Gly Lys Lys Asn Gly Arg Ser Tyr Phe Ala Ala

275 280 285

Glu Glu Thr Ala Pro Pro Val Thr Ala Ala Asn Asn Ala Asp Val Glu

290 295 300

Thr Leu Arg Val Tyr Gly Glu His Pro Phe Phe Thr Leu Leu Gln Gln

305 310 315 320

Arg Ala Ala Leu Gln Trp Pro Gln Leu Arg Val Glu Gln Arg Pro Ala

325 330 335

Leu Pro Gly Leu Gly Ala Ala Val Gln Ile Asn Asp Ala Phe Thr Val

340 345 350

Ser Ile Thr Asp Gly Arg Thr Ala Ser Gln Leu Ala Glu Gln Thr Ala

355 360 365

Ala Asp Ala Phe Val Ile Asp Val Ala Leu Asn Tyr Ala Asp Thr Thr

370 375 380

Tyr Leu Val Ala Ala His Ser Arg His Ala Ser Ala Ala Asn Lys Ala

385 390 395 400

Leu Phe Leu Arg Leu Leu His Thr Ala Ile Pro Gln Val Glu Phe Ile

405 410 415

Lys Asp Ser Pro Ala Leu Ile Val Ala Arg Val Leu Ser Ser Leu Ile

420 425 430

Asn Glu Ser Val Ile Met Val Glu Ser Gly Val Cys Ser Arg Glu Asp

435 440 445

Ile Asp Val Ala Ala Val Ala Gly Val Asn Tyr Ala Gly Gly Ile Phe

450 455 460

Asp Trp Leu Gly Lys Leu Gly Glu Lys Asn Val Arg Thr Thr Leu Ser

465 470 475 480

Asn Leu Ala Gln Leu Leu His Ala Ala Arg Tyr Ala Pro His Tyr Thr

485 490 495

Leu Leu His Ala Ala Gln Pro Ala Leu Thr Thr Thr Pro

500 505

<210> 21

<211> 509

<212> PRT

<213> Serratia marcescens K27

<400> 21

Met Ala Glu Ser Asn Ala Ala Ile Gln Ser Ala Ala Ile Ile Gly Ala

1 5 10 15

Gly Thr Met Gly Arg Gly Ile Ala Tyr Leu Phe Ala Gln Lys Gly Ile

20 25 30

Pro Thr Met Leu Tyr Asn Arg Asn Gly Asn Thr Leu Asn Gln Ala Arg

35 40 45

Glu Tyr Ile Ala Gln Asp Leu Asn Lys Lys Val Glu Gln Gly Lys Ile

50 55 60

Ala Leu Gln Asp Lys Asp Ala Val Leu Ala Asn Leu Met Phe Thr Ser

65 70 75 80

Val Phe Glu Ala Ile Ala Asp Ser Glu Leu Val Ile Glu Thr Ile Ala

85 90 95

Glu Gln Glu Gln Thr Lys Leu Glu Val Leu Ala Ala Ile Ala Ala Val

100 105 110

Val Lys Pro Asp Thr Leu Ile Ala Thr Asn Thr Ser Ser Leu Ser Leu

115 120 125

Asn Lys Leu Ala Thr Ala Val Thr His Ser Glu Arg Phe Ile Gly Leu

130 135 140

His Phe Phe Asn Pro Ala Pro Leu Met Lys Leu Ile Glu Ile Ile Pro

145 150 155 160

Ala Tyr Phe Thr Ala His Ala Thr Thr Glu Arg Cys Arg Gln Leu Val

165 170 175

Ala Ala Leu Gly Lys Arg Asp Val Val Cys Gln Ala Thr Pro Gly Phe

180 185 190

Ile Val Asn Arg Met Ala Arg Pro Tyr Tyr Leu Glu Gly Phe Arg Leu

195 200 205

Leu Glu Glu His Val Ala Arg Ser Ala Gln Ile Asp Arg Ala Leu Lys

210 215 220

Ala Gly Gly Arg Phe Arg Met Gly Pro Leu Glu Leu Thr Asp Phe Ile

225 230 235 240

Gly Gln Asp Ile Asn Tyr Gln Val Ser Arg Gln Ile Trp Gln Asp Met

245 250 255

Gln Tyr Asp Pro Arg Tyr Thr Pro Gly His Leu Gln Arg Ser Leu Val

260 265 270

Asp Ala Gly Leu Leu Gly Lys Lys Asn Gly Arg Ser Tyr Phe Ala Ala

275 280 285

Glu Glu Thr Ala Pro Pro Val Thr Ala Ala Asn Asn Ala Asp Val Glu

290 295 300

Thr Leu Arg Val Tyr Gly Glu His Pro Phe Phe Thr Leu Leu Gln Gln

305 310 315 320

Arg Ala Ala Leu Gln Trp Pro Gln Leu Arg Val Glu Gln Arg Pro Ala

325 330 335

Leu Pro Gly Leu Gly Ala Ala Val Gln Ile Asn Asp Ala Phe Thr Val

340 345 350

Ser Ile Thr Asp Gly Arg Thr Ala Ser Gln Leu Ala Glu Gln Thr Ala

355 360 365

Ala Asp Ala Phe Val Val Asp Val Ala Leu Asn Tyr Ala Asp Thr Thr

370 375 380

Tyr Leu Val Ala Ala His Ser Arg His Ala Ser Ala Ala Asn Lys Ala

385 390 395 400

Leu Phe Leu Arg Leu Leu His Thr Ala Ile Pro Gln Val Glu Phe Ile

405 410 415

Lys Asp Ser Pro Ala Leu Ile Val Ala Arg Val Leu Ser Ser Leu Ile

420 425 430

Asn Glu Ser Val Ile Met Val Glu Ser Gly Val Cys Ser Arg Glu Asp

435 440 445

Ile Asp Val Ala Ala Val Ala Gly Val Asn Tyr Ala Gly Gly Ile Phe

450 455 460

Asp Trp Leu Gly Lys Leu Gly Glu Arg Asn Val Arg Thr Thr Leu Ser

465 470 475 480

Asn Leu Ala Gln Leu Leu His Ala Ala Arg Tyr Ala Pro His Tyr Thr

485 490 495

Leu Leu His Ala Ala Gln Pro Ala Leu Thr Thr Thr Pro

500 505

<210> 22

<211> 509

<212> PRT

<213> Serratia marcescens 280

<400> 22

Met Ala Glu Ser Asn Ala Ala Ile Gln Ser Ala Ala Ile Ile Gly Ala

1 5 10 15

Gly Thr Met Gly Arg Gly Ile Ala Tyr Leu Phe Ala Gln Lys Gly Ile

20 25 30

Pro Thr Met Leu Tyr Asn Arg Asn Gly Asn Thr Leu Asn Gln Ala Arg

35 40 45

Glu Tyr Ile Ala Gln Asp Leu Asn Lys Lys Val Glu Gln Gly Lys Ile

50 55 60

Ala Leu Gln Asp Lys Asp Ala Val Leu Ala Asn Leu Met Phe Thr Ser

65 70 75 80

Val Phe Glu Ala Ile Ala Asp Ser Glu Leu Val Ile Glu Thr Ile Ala

85 90 95

Glu Gln Glu Gln Thr Lys Leu Glu Val Leu Ala Ala Ile Ala Ala Val

100 105 110

Val Lys Pro Asp Thr Leu Ile Ala Thr Asn Thr Ser Ser Leu Ser Leu

115 120 125

Asn Lys Leu Ala Thr Ala Val Thr His Ser Glu Arg Phe Ile Gly Leu

130 135 140

His Phe Phe Asn Pro Ala Pro Leu Met Lys Leu Ile Glu Ile Ile Pro

145 150 155 160

Ala Tyr Phe Thr Ala His Ala Thr Thr Glu Arg Cys Arg Gln Leu Val

165 170 175

Ala Ala Leu Gly Lys Arg Asp Val Val Cys Gln Ala Thr Pro Gly Phe

180 185 190

Ile Val Asn Arg Met Ala Arg Pro Tyr Tyr Leu Glu Gly Phe Arg Leu

195 200 205

Leu Glu Glu His Val Ala Arg Ala Ala Gln Ile Asp Arg Ala Leu Lys

210 215 220

Ala Gly Gly Arg Phe Arg Met Gly Pro Leu Glu Leu Thr Asp Phe Ile

225 230 235 240

Gly Gln Asp Ile Asn Tyr Gln Val Ser Arg Gln Ile Trp Gln Asp Met

245 250 255

Gln Tyr Asp Pro Arg Tyr Thr Pro Gly His Leu Gln Arg Ser Leu Val

260 265 270

Asp Ala Gly Leu Leu Gly Lys Lys Asn Gly Arg Ser Tyr Phe Ala Ala

275 280 285

Glu Glu Thr Ala Pro Pro Val Thr Ala Ala Asn Asn Ala Asp Val Glu

290 295 300

Thr Leu Arg Val Tyr Gly Glu His Pro Phe Phe Thr Leu Leu Gln Gln

305 310 315 320

Arg Ala Ala Leu Gln Trp Pro Gln Leu Arg Val Glu Gln Arg Pro Ala

325 330 335

Leu Pro Gly Leu Gly Ala Ala Val Gln Ile Asn Asp Ala Phe Thr Val

340 345 350

Ser Ile Thr Asp Gly Arg Thr Ala Ser Gln Leu Ala Glu Gln Thr Ala

355 360 365

Ala Asp Ala Phe Val Val Asp Val Ala Leu Asn Tyr Ala Asp Thr Thr

370 375 380

Tyr Leu Val Ala Ala His Ser Arg His Ala Ser Ala Ala Asn Lys Ala

385 390 395 400

Leu Phe Leu Leu Leu Leu His Thr Ala Ile Pro Gln Val Glu Phe Ile

405 410 415

Lys Asp Ser Pro Ala Leu Ile Val Ala Arg Val Leu Ser Ser Leu Ile

420 425 430

Asn Glu Ser Val Ile Met Val Glu Ser Gly Val Cys Ser Arg Glu Asp

435 440 445

Ile Asp Val Ala Ala Val Ala Gly Val Asn Tyr Ala Gly Gly Ile Phe

450 455 460

Asp Trp Leu Gly Lys Leu Gly Glu Lys Asn Val Arg Thr Thr Leu Ser

465 470 475 480

Asn Leu Ala Gln Leu Leu His Ala Ala Arg Tyr Ala Pro His Tyr Thr

485 490 495

Leu Leu His Ala Ala Gln Pro Ala Leu Thr Thr Thr Pro

500 505

<210> 23

<211> 509

<212> PRT

<213> Serratia marcescens 19F

<400> 23

Met Ala Glu Ser Asn Ala Ala Ile Gln Ser Ala Ala Ile Ile Gly Ala

1 5 10 15

Gly Thr Met Gly Arg Gly Ile Ala Tyr Leu Phe Ala Gln Lys Gly Ile

20 25 30

Pro Thr Met Leu Tyr Asn Arg Asn Gly Asn Thr Leu Asn Gln Ala Arg

35 40 45

Glu Tyr Ile Ala Gln Asp Leu Asn Lys Lys Val Glu Gln Gly Lys Ile

50 55 60

Ala Leu Gln Asp Lys Asp Ala Val Leu Ala Asn Leu Met Phe Thr Ser

65 70 75 80

Val Phe Glu Ala Ile Ala Asp Ser Glu Leu Val Ile Glu Thr Ile Ala

85 90 95

Glu Gln Glu Gln Thr Lys Leu Glu Val Leu Ala Ala Ile Ala Ala Val

100 105 110

Val Lys His Asp Thr Leu Ile Ala Thr Asn Thr Ser Ser Leu Ser Leu

115 120 125

Asn Lys Leu Ala Thr Ala Val Thr His Ser Glu Arg Phe Ile Gly Leu

130 135 140

His Phe Phe Asn Pro Ala Pro Leu Met Lys Leu Ile Glu Ile Ile Pro

145 150 155 160

Ala Tyr Phe Thr Ala His Ala Thr Thr Glu Arg Cys Arg Gln Leu Val

165 170 175

Ala Ala Leu Gly Lys Arg Asp Val Val Cys Gln Ala Thr Pro Gly Phe

180 185 190

Ile Val Asn Arg Met Ala Arg Pro Tyr Tyr Leu Glu Gly Phe Arg Leu

195 200 205

Leu Glu Glu His Val Ala Arg Ala Ala Gln Ile Asp Arg Ala Leu Lys

210 215 220

Ala Gly Gly Arg Phe Arg Met Gly Pro Leu Glu Leu Thr Asp Phe Ile

225 230 235 240

Gly Gln Asp Ile Asn Tyr Gln Val Ser Arg Gln Ile Trp Gln Asp Met

245 250 255

Gln Tyr Asp Pro Arg Tyr Thr Pro Gly His Leu Gln Arg Ser Leu Val

260 265 270

Asp Ala Gly Leu Leu Gly Lys Lys Asn Gly Arg Ser Tyr Phe Ala Ala

275 280 285

Glu Glu Thr Ala Pro Pro Val Thr Ala Ala Asn Asn Ala Asp Val Glu

290 295 300

Thr Leu Arg Val Tyr Gly Glu His Pro Phe Phe Thr Leu Leu Gln Gln

305 310 315 320

Arg Ala Ala Leu Gln Trp Pro Gln Leu Arg Val Glu Gln Arg Pro Ala

325 330 335

Leu Pro Gly Leu Gly Ala Ala Val Gln Ile Asn Asp Ala Phe Thr Val

340 345 350

Ser Ile Thr Asp Gly Arg Thr Ala Ser Gln Leu Ala Glu Gln Thr Ala

355 360 365

Ala Asp Ala Phe Val Val Asp Val Ala Leu Asn Tyr Ala Asp Thr Thr

370 375 380

Tyr Leu Val Ala Ala His Ser Arg His Ala Ser Ala Ala Asn Lys Ala

385 390 395 400

Leu Phe Leu Arg Leu Leu His Thr Ala Ile Pro Gln Val Glu Phe Ile

405 410 415

Lys Asp Ser Pro Ala Leu Ile Val Ala Arg Val Leu Ser Ser Leu Ile

420 425 430

Asn Glu Ser Val Ile Met Val Glu Ser Gly Val Cys Ser Arg Glu Asp

435 440 445

Ile Asp Val Ala Ala Val Ala Gly Val Asn Tyr Ala Gly Gly Ile Phe

450 455 460

Asp Trp Leu Gly Lys Leu Gly Glu Lys Asn Val Arg Thr Thr Leu Ser

465 470 475 480

Asn Leu Ala Gln Leu Leu His Ala Ala Arg Tyr Ala Pro His Tyr Thr

485 490 495

Leu Leu His Ala Ala Gln Pro Ala Leu Thr Thr Thr Pro

500 505

<210> 24

<211> 509

<212> PRT

<213> Serratia marcescens 1185

<400> 24

Met Ala Glu Ser Asn Ala Ala Ile Gln Ser Ala Ala Ile Ile Gly Ala

1 5 10 15

Gly Thr Met Gly Arg Gly Ile Ala Tyr Leu Phe Ala Gln Lys Gly Ile

20 25 30

Pro Thr Met Leu Tyr Asn Arg Asn Gly Asn Thr Leu Asn Gln Ala Arg

35 40 45

Glu Tyr Ile Ala Gln Asp Leu Asn Lys Lys Val Glu Gln Gly Lys Ile

50 55 60

Ala Leu Arg Asp Lys Asp Ala Val Leu Ala Asn Leu Met Phe Thr Ser

65 70 75 80

Val Phe Glu Ala Ile Ala Asp Ser Glu Leu Val Ile Glu Thr Ile Ala

85 90 95

Glu Gln Glu Gln Thr Lys Leu Glu Val Leu Ala Ala Ile Ala Ala Val

100 105 110

Val Lys Pro Asp Thr Leu Ile Ala Thr Asn Thr Ser Ser Leu Ser Leu

115 120 125

Asn Lys Leu Ala Thr Ala Val Thr His Ser Glu Arg Phe Ile Gly Leu

130 135 140

His Phe Phe Asn Pro Ala Pro Leu Met Lys Leu Ile Glu Ile Ile Pro

145 150 155 160

Ala Tyr Phe Thr Ala His Ala Thr Thr Glu Arg Cys Arg Gln Leu Val

165 170 175

Ala Ala Leu Gly Lys His Asp Val Val Cys Gln Ala Thr Pro Gly Phe

180 185 190

Ile Val Asn Arg Met Ala Arg Pro Tyr Tyr Leu Glu Gly Phe Arg Leu

195 200 205

Leu Glu Glu His Val Ala Arg Ala Ala Gln Ile Asp Arg Ala Leu Lys

210 215 220

Ala Gly Gly Arg Phe Arg Met Gly Pro Leu Glu Leu Thr Asp Phe Ile

225 230 235 240

Gly Gln Asp Ile Asn Tyr Gln Val Ser Arg Gln Ile Trp Gln Asp Met

245 250 255

Gln Tyr Asp Pro Arg Tyr Thr Pro Gly His Leu Gln Arg Ser Leu Val

260 265 270

Asp Ala Gly Leu Leu Gly Lys Lys Asn Gly Arg Ser Tyr Phe Ala Ala

275 280 285

Glu Glu Thr Ala Pro Pro Val Thr Ala Ala Asn Asn Ala Asp Val Glu

290 295 300

Thr Leu Arg Val Tyr Gly Glu His Pro Phe Phe Thr Leu Leu Gln Gln

305 310 315 320

Arg Ala Ala Leu Gln Trp Pro Gln Leu Arg Val Glu Gln Arg Pro Ala

325 330 335

Leu Pro Gly Leu Gly Ala Ala Val Gln Ile Asn Asp Ala Phe Thr Val

340 345 350

Ser Ile Thr Asp Gly Arg Thr Ala Ser Gln Leu Ala Glu Gln Thr Ala

355 360 365

Ala Asp Ala Phe Val Val Asp Leu Ala Leu Asn Tyr Ala Asp Thr Thr

370 375 380

Tyr Leu Val Ala Ala His Ser Arg His Ala Ser Ala Ala Asn Lys Ala

385 390 395 400

Leu Phe Leu Arg Leu Leu His Thr Ala Ile Pro Gln Val Glu Phe Ile

405 410 415

Lys Asp Ser Pro Ala Leu Ile Val Ala Arg Val Leu Ser Ser Leu Ile

420 425 430

Asn Glu Ser Val Ile Met Val Glu Ser Gly Val Cys Ser Arg Glu Asp

435 440 445

Ile Asp Val Ala Ala Val Ala Gly Val Asn Tyr Ala Gly Gly Ile Phe

450 455 460

Asp Trp Leu Gly Lys Leu Gly Glu Lys Asn Val Arg Thr Thr Leu Ser

465 470 475 480

Asn Leu Ala Gln Leu Leu His Ala Ala Arg Tyr Ala Pro His Tyr Thr

485 490 495

Leu Leu His Ala Ala Gln Ser Ala Leu Thr Thr Thr Pro

500 505

<210> 25

<211> 509

<212> PRT

<213> Serratia marcescens S2I7

<400> 25

Met Ala Glu Ser Asn Ala Ala Ile Gln Ser Ala Ala Ile Ile Gly Ala

1 5 10 15

Gly Thr Met Gly Arg Gly Ile Ala Tyr Leu Phe Ala Gln Lys Gly Ile

20 25 30

Pro Thr Met Leu Tyr Asn Arg Asn Gly Asn Thr Leu Asn Gln Ala Arg

35 40 45

Glu Tyr Ile Ala Gln Asp Leu Asn Lys Lys Val Glu Gln Gly Lys Ile

50 55 60

Ala Leu Gln Asp Lys Asp Ala Val Leu Ala Asn Leu Met Phe Thr Ser

65 70 75 80

Val Phe Glu Ala Ile Ala Asp Ser Glu Leu Val Ile Glu Thr Ile Ala

85 90 95

Glu Gln Glu Gln Thr Lys Leu Glu Val Leu Ala Ala Ile Ala Ala Val

100 105 110

Val Lys Pro Asp Thr Leu Ile Ala Thr Asn Thr Ser Ser Leu Ser Leu

115 120 125

Asn Lys Leu Ala Thr Ala Val Thr His Ser Glu Arg Phe Ile Gly Leu

130 135 140

His Phe Phe Asn Pro Ala Pro Leu Met Lys Leu Ile Glu Ile Ile Pro

145 150 155 160

Ala Tyr Phe Thr Ala His Ala Thr Thr Glu Arg Cys Arg Gln Leu Val

165 170 175

Ala Ala Leu Gly Lys Arg Asp Val Val Cys Gln Ala Thr Pro Gly Phe

180 185 190

Ile Val Asn Arg Met Ala Arg Pro Tyr Tyr Leu Glu Gly Phe Arg Leu

195 200 205

Leu Glu Glu His Val Ala Arg Ala Ala Gln Ile Asp Arg Ala Leu Lys

210 215 220

Ala Gly Gly Arg Phe Arg Met Gly Pro Leu Glu Leu Thr Asp Phe Ile

225 230 235 240

Gly Gln Asp Ile Asn Tyr Gln Val Ser Arg Gln Ile Trp Gln Asp Met

245 250 255

Gln Tyr Asp Pro Arg Tyr Thr Pro Gly His Leu Gln Arg Ser Leu Val

260 265 270

Asp Ala Gly Leu Leu Gly Lys Lys Asn Gly Arg Ser Tyr Phe Ala Ala

275 280 285

Glu Glu Thr Ala Pro Pro Val Thr Ala Ala Asn Asn Ala Asp Val Glu

290 295 300

Thr Leu Arg Val Tyr Gly Glu His Pro Phe Phe Thr Leu Leu Gln Gln

305 310 315 320

Arg Ala Ala Leu Gln Trp Pro Gln Leu Cys Val Glu Gln Arg Pro Ala

325 330 335

Leu Pro Gly Leu Gly Ala Ala Val Gln Ile Asn Asp Ala Phe Thr Val

340 345 350

Ser Ile Thr Asp Gly Arg Thr Ala Ser Gln Leu Ala Glu Gln Thr Ala

355 360 365

Ala Asp Ala Phe Val Val Asp Val Ala Leu Asn Tyr Ala Asp Thr Thr

370 375 380

Tyr Leu Val Ala Ala His Ser Arg His Ala Ser Ala Ala Asn Lys Ala

385 390 395 400

Leu Phe Leu Arg Leu Leu His Thr Ala Ile Pro Gln Val Glu Phe Ile

405 410 415

Lys Asp Ser Pro Ala Leu Ile Val Ala Arg Val Leu Ser Ser Leu Ile

420 425 430

Asn Glu Ser Val Ile Met Val Glu Ser Gly Val Cys Ser Arg Glu Asp

435 440 445

Ile Asp Val Ala Ala Val Ala Gly Val Asn Tyr Ala Gly Gly Ile Phe

450 455 460

Asp Trp Leu Gly Lys Leu Gly Glu Arg Asn Val Arg Thr Thr Leu Ser

465 470 475 480

Asn Leu Ala Gln Leu Leu His Ala Ala Arg Tyr Ala Pro His Tyr Thr

485 490 495

Leu Leu His Ala Ala Gln Pro Ala Leu Thr Thr Thr Pro

500 505

<210> 26

<211> 509

<212> PRT

<213> Serratia marcescens KHCo-24B

<400> 26

Met Ala Glu Ser Asn Ala Ala Ile Gln Ser Ala Ala Ile Ile Gly Ala

1 5 10 15

Gly Thr Met Gly Arg Gly Ile Ala Tyr Leu Phe Ala Gln Lys Gly Ile

20 25 30

Pro Thr Met Leu Tyr Asn Arg Asn Gly Asn Thr Leu Asn Gln Ala Arg

35 40 45

Glu Tyr Ile Ala Gln Asp Leu Asn Lys Lys Val Glu Gln Gly Lys Ile

50 55 60

Ala Leu Arg Asp Lys Asp Ala Val Leu Ala Asn Leu Met Phe Thr Ser

65 70 75 80

Val Phe Glu Ala Ile Ala Asp Ser Glu Leu Val Ile Glu Thr Ile Ala

85 90 95

Glu Gln Glu Gln Thr Lys Leu Glu Val Leu Ala Ala Ile Ala Ala Val

100 105 110

Val Lys Pro Asp Thr Leu Ile Ala Thr Asn Thr Ser Ser Leu Ser Leu

115 120 125

Asn Lys Leu Ala Thr Ala Val Thr His Ser Glu Arg Phe Ile Gly Leu

130 135 140

His Phe Phe Asn Pro Ala Pro Leu Met Lys Leu Ile Glu Ile Ile Pro

145 150 155 160

Ala Tyr Phe Thr Ala His Ala Thr Thr Glu Arg Cys Arg Gln Leu Val

165 170 175

Ala Ala Leu Gly Lys Arg Asp Val Val Cys Gln Ala Thr Pro Gly Phe

180 185 190

Ile Val Asn Arg Met Ala Arg Pro Tyr Tyr Leu Glu Gly Phe Arg Leu

195 200 205

Leu Glu Glu His Val Ala Arg Ala Ala Gln Ile Asp Arg Ala Leu Lys

210 215 220

Ala Gly Gly Arg Phe Arg Met Gly Pro Leu Glu Leu Thr Asp Phe Ile

225 230 235 240

Gly Gln Asp Ile Asn Tyr Gln Val Ser Arg Gln Ile Trp Gln Asp Met

245 250 255

Gln Tyr Asp Pro Arg Tyr Thr Pro Gly His Leu Gln Arg Ser Leu Val

260 265 270

Asp Ala Gly Leu Leu Gly Lys Lys Asn Gly Arg Ser Tyr Phe Ala Ala

275 280 285

Glu Glu Thr Ala Pro Pro Val Thr Ala Ala Asn Asn Ala Asp Val Glu

290 295 300

Thr Leu Arg Val Tyr Gly Glu His Pro Phe Phe Thr Leu Leu Gln Gln

305 310 315 320

Arg Ala Ala Leu Gln Trp Pro Gln Leu Arg Val Glu Gln Arg Pro Ala

325 330 335

Leu Pro Gly Leu Gly Ala Ala Val Gln Ile Asn Asp Ala Phe Thr Val

340 345 350

Ser Ile Thr Asp Gly Arg Thr Ala Ser Gln Leu Ala Glu Gln Ala Ala

355 360 365

Ala Asp Ala Phe Val Val Asp Leu Ala Leu Asn Tyr Ala Asp Thr Thr

370 375 380

Tyr Leu Val Ala Ala His Ser Arg His Ala Ser Ala Ala Asn Lys Ala

385 390 395 400

Leu Phe Leu Arg Leu Leu His Thr Ala Ile Pro Gln Val Glu Phe Ile

405 410 415

Lys Asp Ser Pro Ala Leu Ile Val Ala Arg Val Leu Ser Ser Leu Ile

420 425 430

Asn Glu Ser Val Ile Met Val Glu Ser Gly Val Cys Ser Arg Glu Asp

435 440 445

Ile Asp Val Ala Ala Val Ala Gly Val Asn Tyr Ala Gly Gly Ile Phe

450 455 460

Asp Trp Leu Gly Lys Leu Gly Glu Lys Asn Val Arg Thr Thr Leu Ser

465 470 475 480

Asn Leu Ala Gln Leu Leu His Ala Ala Arg Tyr Ala Pro His Tyr Thr

485 490 495

Leu Leu His Ala Ala Gln Pro Ala Leu Thr Thr Thr Pro

500 505

<210> 27

<211> 509

<212> PRT

<213> Serratia marcescens Z6

<400> 27

Met Ala Glu Ser Asn Ala Ala Ile Gln Ser Ala Ala Ile Ile Gly Ala

1 5 10 15

Gly Thr Met Gly Arg Gly Ile Ala Tyr Leu Phe Ala Gln Lys Gly Ile

20 25 30

Pro Thr Met Leu Tyr Asn Arg Asn Gly Asn Thr Leu Asn Gln Ala Arg

35 40 45

Glu Tyr Ile Ala Gln Asp Leu Asn Lys Lys Val Glu Gln Gly Lys Ile

50 55 60

Ala Leu Gln Asp Lys Asp Ala Val Leu Ala Asn Leu Met Phe Thr Ser

65 70 75 80

Val Phe Glu Ala Ile Ala Asp Ser Glu Leu Val Ile Glu Thr Ile Ala

85 90 95

Glu Gln Glu Gln Thr Lys Leu Glu Val Leu Ala Ala Ile Ala Ala Val

100 105 110

Val Lys Pro Asp Thr Leu Ile Ala Thr Asn Thr Ser Ser Leu Ser Leu

115 120 125

Asn Lys Leu Ala Thr Ala Val Thr His Arg Glu Arg Phe Ile Gly Leu

130 135 140

His Phe Phe Asn Pro Ala Pro Leu Met Lys Leu Ile Glu Ile Ile Pro

145 150 155 160

Ala Tyr Phe Thr Ala His Ala Thr Thr Glu Arg Cys Arg Gln Leu Val

165 170 175

Ala Ala Leu Gly Lys Arg Asp Val Val Cys Gln Ala Thr Pro Gly Phe

180 185 190

Ile Val Asn Arg Met Ala Arg Pro Tyr Tyr Leu Glu Gly Phe Arg Leu

195 200 205

Leu Glu Glu His Val Ala Arg Ala Ala Gln Ile Asp Arg Ala Leu Lys

210 215 220

Ala Gly Gly Arg Phe Arg Met Gly Pro Leu Glu Leu Thr Asp Phe Ile

225 230 235 240

Gly Gln Asp Ile Asn Tyr Gln Val Ser Arg Gln Ile Trp Gln Asp Met

245 250 255

Gln Tyr Asp Pro Arg Tyr Thr Pro Gly His Leu Gln Arg Ser Leu Val

260 265 270

Asp Ala Gly Leu Leu Gly Lys Lys Asn Gly Arg Ser Tyr Phe Ala Ala

275 280 285

Glu Glu Thr Ala Pro Pro Val Thr Ala Ala Asn Asn Ala Asp Val Glu

290 295 300

Thr Leu Arg Val Tyr Gly Glu His Pro Phe Phe Thr Leu Leu Gln Gln

305 310 315 320

Arg Ala Ala Leu Gln Trp Pro Gln Leu Cys Val Glu Gln Arg Pro Ala

325 330 335

Leu Pro Gly Leu Gly Ala Ala Val Gln Ile Asn Asp Ala Phe Thr Val

340 345 350

Ser Ile Thr Asp Gly Arg Thr Ala Ser Gln Leu Ala Glu Gln Thr Ala

355 360 365

Ala Asp Ala Phe Val Ile Asp Val Ala Leu Asn Tyr Ala Asp Thr Thr

370 375 380

Tyr Leu Val Ala Ala His Ser Arg His Ala Ser Ala Ala Asn Lys Ala

385 390 395 400

Leu Phe Leu Arg Leu Leu His Thr Ala Ile Pro Gln Val Glu Phe Ile

405 410 415

Lys Asp Ser Pro Ala Leu Ile Val Ala Arg Val Leu Ser Ser Leu Ile

420 425 430

Asn Glu Ser Val Ile Met Val Glu Ser Gly Val Cys Ser Arg Glu Asp

435 440 445

Ile Asp Val Ala Ala Val Ala Gly Val Asn Tyr Ala Gly Gly Ile Phe

450 455 460

Asp Trp Leu Gly Lys Leu Gly Glu Lys Asn Val Arg Thr Thr Leu Ser

465 470 475 480

Asn Leu Ala Gln Leu Leu His Ala Ala Arg Tyr Ala Pro His Tyr Thr

485 490 495

Leu Leu His Ala Ala Gln Pro Ala Leu Thr Thr Thr Pro

500 505

<210> 28

<211> 509

<212> PRT

<213> Serratia marcescens 546

<400> 28

Met Ala Glu Ser Asn Ala Ala Ile Gln Ser Ala Ala Ile Ile Gly Ala

1 5 10 15

Gly Thr Met Gly Arg Gly Ile Ala Tyr Leu Phe Ala Gln Lys Gly Ile

20 25 30

Pro Thr Met Leu Tyr Asn Arg Asn Gly Asn Thr Leu Asn Gln Ala Arg

35 40 45

Glu Tyr Ile Ala Gln Asp Leu Asn Lys Lys Val Glu Gln Gly Lys Ile

50 55 60

Ala Leu Arg Asp Lys Asp Ala Val Leu Ala Asn Leu Met Phe Thr Ser

65 70 75 80

Val Phe Glu Ala Ile Ala Asp Ser Glu Leu Val Ile Glu Thr Ile Ala

85 90 95

Glu Gln Glu Gln Thr Lys Leu Glu Val Leu Ala Ala Ile Ala Ala Val

100 105 110

Val Lys Pro Asp Thr Leu Ile Ala Thr Asn Thr Ser Ser Leu Ser Leu

115 120 125

Asn Lys Leu Ser Thr Ala Val Thr His Asn Glu Arg Phe Ile Gly Leu

130 135 140

His Phe Phe Asn Pro Ala Pro Leu Met Lys Leu Ile Glu Ile Ile Pro

145 150 155 160

Ala Tyr Phe Thr Ala His Ala Thr Thr Glu Arg Cys Arg Gln Leu Val

165 170 175

Ala Ala Leu Gly Lys Arg Asp Val Val Cys Gln Ala Thr Pro Gly Phe

180 185 190

Ile Val Asn Arg Met Ala Arg Pro Tyr Tyr Leu Glu Gly Phe Arg Leu

195 200 205

Leu Glu Glu His Val Ala Arg Ala Ala Gln Ile Asp Arg Ala Leu Lys

210 215 220

Ala Gly Gly Arg Phe Arg Met Gly Pro Leu Glu Leu Thr Asp Phe Ile

225 230 235 240

Gly Gln Asp Ile Asn Tyr Gln Val Ser Arg Gln Ile Trp Gln Asp Met

245 250 255

Gln Tyr Asp Pro Arg Tyr Thr Pro Gly His Leu Gln Arg Ser Leu Val

260 265 270

Asp Ala Gly Leu Leu Gly Lys Lys Asn Gly Arg Ser Tyr Phe Ala Ala

275 280 285

Glu Glu Thr Ala Pro Pro Val Thr Ala Ala Asn Asn Ala Asp Val Glu

290 295 300

Thr Leu Arg Val Tyr Gly Glu His Pro Phe Phe Thr Leu Leu Gln Gln

305 310 315 320

Arg Ala Ala Leu Gln Trp Pro Gln Leu Arg Val Glu Gln Arg Pro Ala

325 330 335

Leu Pro Gly Leu Gly Ala Ala Val Arg Ile Asn Asp Ala Phe Thr Val

340 345 350

Ser Ile Thr Asp Gly Arg Thr Ala Ser Gln Leu Ala Glu Gln Thr Ala

355 360 365

Ala Asp Ala Phe Val Val Asp Val Ala Leu Asn Tyr Ala Asp Thr Thr

370 375 380

Tyr Leu Val Ala Ala His Ser Arg His Ala Ser Ala Ala Asn Lys Ala

385 390 395 400

Leu Phe Leu Arg Leu Leu His Thr Ala Ile Pro Gln Val Glu Phe Ile

405 410 415

Lys Asp Ser Pro Ala Leu Ile Val Ala Arg Val Leu Ser Ser Leu Ile

420 425 430

Asn Glu Ser Val Ile Met Val Glu Ser Gly Val Cys Ser Arg Glu Asp

435 440 445

Ile Asp Val Ala Ala Val Ala Gly Val Asn Tyr Ala Gly Gly Ile Phe

450 455 460

Asp Trp Leu Gly Lys Leu Gly Glu Lys Asn Val Arg Thr Thr Leu Ser

465 470 475 480

Asn Leu Ala Gln Leu Leu His Ala Ala Arg Tyr Ala Pro His Tyr Thr

485 490 495

Leu Leu His Ala Ala Gln Pro Ala Leu Thr Thr Thr Pro

500 505

<210> 29

<211> 509

<212> PRT

<213> Serratia nematodiphila MB307

<400> 29

Met Ala Glu Ser Asn Ala Ala Ile Gln Ser Ala Ala Ile Ile Gly Ala

1 5 10 15

Gly Thr Met Gly Arg Gly Ile Ala Tyr Leu Phe Ala Gln Lys Gly Ile

20 25 30

Pro Thr Met Leu Tyr Asn Arg Asn Gly Asn Thr Leu Asn Gln Ala Arg

35 40 45

Glu Tyr Ile Ala Gln Asp Leu Asn Lys Lys Val Glu Gln Gly Lys Ile

50 55 60

Ala Leu Arg Asp Lys Asp Ala Val Leu Ala Asn Leu Met Phe Thr Ser

65 70 75 80

Val Phe Glu Ala Ile Ala Asp Ser Glu Leu Val Ile Glu Thr Ile Ala

85 90 95

Glu Gln Glu Gln Thr Lys Leu Glu Val Leu Ala Ala Ile Ala Ala Val

100 105 110

Val Lys Pro Asp Thr Leu Ile Ala Thr Asn Thr Ser Ser Leu Ser Leu

115 120 125

Asn Lys Leu Ala Thr Ala Val Thr His Ser Glu Arg Phe Ile Gly Leu

130 135 140

His Phe Phe Asn Pro Ala Pro Leu Met Lys Leu Ile Glu Ile Ile Pro

145 150 155 160

Ala Tyr Phe Thr Ala His Ala Thr Thr Glu Arg Cys Arg Gln Leu Val

165 170 175

Ala Ala Leu Gly Lys Arg Asp Val Val Cys Gln Ala Thr Pro Gly Phe

180 185 190

Ile Val Asn Arg Met Ala Arg Pro Tyr Tyr Leu Glu Gly Phe Arg Leu

195 200 205

Leu Glu Glu His Val Ala Arg Ala Ala Gln Ile Asp Arg Ala Leu Lys

210 215 220

Ala Gly Gly Arg Phe Arg Met Gly Pro Leu Glu Leu Thr Asp Phe Ile

225 230 235 240

Gly Gln Asp Ile Asn Tyr Gln Val Ser Arg Gln Ile Trp Gln Asp Met

245 250 255

Gln Tyr Asp Pro Arg Tyr Thr Pro Gly His Leu Gln Arg Ser Leu Val

260 265 270

Asp Ala Gly Leu Leu Gly Lys Lys Asn Gly Arg Ser Tyr Phe Ala Ala

275 280 285

Glu Glu Thr Ala Pro Gln Val Thr Ala Ala Asn Asn Ala Asp Val Glu

290 295 300

Thr Leu Arg Val Tyr Gly Glu His Pro Phe Phe Thr Leu Leu Gln Gln

305 310 315 320

Arg Ala Ala Leu Gln Trp Pro Gln Leu Arg Val Glu Gln Arg Pro Ala

325 330 335

Leu Pro Gly Leu Gly Ala Ala Val Gln Ile Asn Asp Ala Phe Thr Val

340 345 350

Ser Ile Thr Asp Gly Arg Thr Ala Ser Gln Leu Ala Glu Gln Thr Ala

355 360 365

Ala Asp Ala Phe Val Val Asp Leu Ala Leu Asn Tyr Ala Asp Thr Thr

370 375 380

Tyr Leu Val Ala Ala His Ser Arg His Ala Ser Ala Ala Asn Lys Ala

385 390 395 400

Leu Phe Leu Arg Leu Leu His Thr Ala Ile Pro Gln Val Glu Phe Ile

405 410 415

Lys Asp Ser Pro Ala Leu Ile Val Ala Arg Val Leu Ser Ser Leu Ile

420 425 430

Asn Glu Ser Val Ile Met Val Glu Ser Gly Val Cys Ser Arg Glu Asp

435 440 445

Ile Asp Val Ala Ala Val Ala Gly Val Asn Tyr Ala Gly Gly Ile Phe

450 455 460

Asp Trp Leu Gly Lys Leu Gly Glu Lys Asn Val Arg Thr Thr Leu Ser

465 470 475 480

Asn Leu Ala Gln Leu Leu His Ala Ala Arg Tyr Ala Pro His Tyr Thr

485 490 495

Leu Leu His Ala Ala Gln Pro Ala Leu Thr Thr Thr Pro

500 505

<210> 30

<211> 509

<212> PRT

<213> Serratia marcescens VGH107

<400> 30

Met Ala Glu Ser Asn Ala Ala Ile Gln Ser Ala Ala Ile Ile Gly Ala

1 5 10 15

Gly Thr Met Gly Arg Gly Ile Ala Tyr Leu Phe Ala Gln Lys Gly Ile

20 25 30

Pro Thr Met Leu Tyr Asn Arg Asn Gly Asn Thr Leu Asn Gln Ala Arg

35 40 45

Glu Tyr Ile Ala Gln Asp Leu Asn Lys Lys Val Glu Gln Gly Lys Ile

50 55 60

Ala Leu Gln Asp Lys Asp Ala Val Leu Ala Asn Leu Met Phe Thr Ser

65 70 75 80

Val Phe Glu Ala Ile Ala Asp Ser Glu Leu Val Ile Glu Thr Ile Ala

85 90 95

Glu Gln Glu Gln Thr Lys Leu Glu Val Leu Ala Ala Ile Ala Ala Val

100 105 110

Val Lys His Asp Thr Leu Ile Ala Thr Asn Thr Ser Ser Leu Ser Leu

115 120 125

Asn Lys Leu Ala Thr Ala Val Thr His Ser Glu Arg Phe Ile Gly Leu

130 135 140

His Phe Phe Asn Pro Ala Pro Leu Met Lys Leu Ile Glu Ile Ile Pro

145 150 155 160

Ala Tyr Phe Thr Ala His Ala Thr Thr Glu Arg Cys Arg Gln Leu Val

165 170 175

Ala Ala Leu Gly Lys Arg Asp Val Val Cys Gln Ala Thr Pro Gly Phe

180 185 190

Ile Val Asn Arg Met Ala Arg Pro Tyr Tyr Leu Glu Gly Phe Arg Leu

195 200 205

Leu Glu Glu His Val Ala Arg Ala Ala Gln Ile Asp Arg Ala Leu Lys

210 215 220

Ala Gly Gly Arg Phe Arg Met Gly Pro Leu Glu Leu Thr Asp Phe Ile

225 230 235 240

Gly Gln Asp Ile Asn Tyr Gln Val Ser Arg Gln Ile Trp Gln Asp Met

245 250 255

Gln Tyr Asp Pro Arg Tyr Thr Pro Gly His Leu Gln Arg Ser Leu Val

260 265 270

Asp Ala Gly Leu Leu Gly Lys Lys Asn Gly Arg Ser Tyr Phe Ala Ala

275 280 285

Glu Glu Thr Ala Pro Pro Val Thr Ala Ala Asn Asn Ala Asp Val Glu

290 295 300

Thr Leu Arg Val Tyr Gly Glu His Pro Phe Phe Thr Leu Leu Gln Gln

305 310 315 320

Arg Ala Ala Leu Gln Trp Pro Gln Leu Arg Val Glu Gln Arg Pro Ala

325 330 335

Leu Pro Gly Leu Gly Ala Ala Val Gln Ile Asn Asp Ala Phe Thr Val

340 345 350

Ser Ile Thr Asp Gly Arg Thr Ala Ser Gln Leu Ala Glu Gln Thr Ala

355 360 365

Ala Asp Ala Phe Val Val Asp Val Ala Leu Asn Tyr Ala Asp Thr Thr

370 375 380

Tyr Leu Val Ala Ala His Ser Arg His Ala Ser Ala Ala Asn Lys Ala

385 390 395 400

Leu Phe Leu Arg Leu Leu His Thr Ala Ile Pro Gln Val Glu Phe Ile

405 410 415

Lys Asp Ser Pro Ala Leu Ile Val Ala Arg Val Leu Ser Ser Leu Ile

420 425 430

Asn Glu Ser Val Ile Met Val Glu Ser Gly Val Cys Ser Arg Glu Asp

435 440 445

Ile Asp Val Ala Ala Val Ala Gly Val Asn Tyr Ala Gly Gly Ile Phe

450 455 460

Asp Trp Leu Gly Lys Leu Gly Glu Lys Asn Val Arg Thr Met Leu Ser

465 470 475 480

Asn Leu Ala Gln Leu Leu His Ala Ala Arg Tyr Ala Pro His Tyr Asn

485 490 495

Leu Leu His Ala Ala Gln Pro Ala Leu Thr Thr Thr Pro

500 505

<210> 31

<211> 509

<212> PRT

<213> Serratia marcescens MCB

<400> 31

Met Ala Glu Ser Asn Ala Ala Ile Gln Ser Ala Ala Ile Ile Gly Ala

1 5 10 15

Gly Thr Met Gly Arg Gly Ile Ala Tyr Leu Phe Ala Gln Lys Gly Ile

20 25 30

Arg Thr Val Leu Tyr Asn Arg Asn Gly Asn Thr Leu Asn Gln Ala Arg

35 40 45

Glu Tyr Ile Ala Gln Asp Leu Asn Lys Lys Val Glu Gln Gly Lys Ile

50 55 60

Ala Leu Gln Asp Lys Ser Ser Val Leu Ala Asn Leu Val Phe Ser Thr

65 70 75 80

Ala Leu Glu Ala Ile Ala Asp Ser Glu Leu Val Ile Glu Thr Ile Ala

85 90 95

Glu His Glu Gln Thr Lys Leu Glu Val Leu Ala Ala Ile Ala Ala Val

100 105 110

Val Lys Pro Asp Thr Leu Ile Ala Thr Asn Thr Ser Ser Leu Ser Leu

115 120 125

Asn Lys Leu Ala Thr Ala Val Thr His Ser Glu Arg Phe Ile Gly Leu

130 135 140

His Phe Phe Asn Pro Ala Pro Leu Met Lys Leu Ile Glu Ile Ile Pro

145 150 155 160

Ala Tyr Phe Thr Ala His Ala Thr Thr Glu Arg Cys Arg Gln Leu Val

165 170 175

Ala Ala Leu Gly Lys Arg Asp Val Val Cys Gln Ala Thr Pro Gly Phe

180 185 190

Ile Val Asn Arg Met Ala Arg Pro Tyr Tyr Leu Glu Gly Phe Arg Leu

195 200 205

Leu Glu Glu His Val Ala Arg Ala Pro Gln Ile Asp Arg Ala Leu Lys

210 215 220

Ala Gly Gly His Phe Arg Met Gly Pro Leu Glu Leu Thr Asp Phe Ile

225 230 235 240

Gly Gln Asp Ile Asn Tyr Gln Val Ser Arg Gln Ile Trp Gln Asp Met

245 250 255

Gln Tyr Asp Pro Arg Tyr Thr Pro Gly His Leu Gln Arg Ser Leu Val

260 265 270

Asp Ala Gly Leu Leu Gly Lys Lys Asn Gly Arg Ser Tyr Phe Thr Ala

275 280 285

Glu Glu Thr Ala Pro Pro Val Thr Ala Ala Ile Gly Ala Asp Val Glu

290 295 300

Thr Leu Arg Ile Tyr Gly Glu His Pro Phe Phe Thr Leu Leu Gln Gln

305 310 315 320

Arg Ala Ala Leu Gln Trp Pro Gln Leu Arg Val Glu Gln Arg Pro Ala

325 330 335

Leu Pro Gly Val Gly Pro Ala Val Gln Ile Asn Asp Ala Phe Thr Val

340 345 350

Ser Ile Thr Asp Gly Arg Thr Ala Ser Gln Leu Ala Glu Gln Thr Thr

355 360 365

Ala Asp Ala Phe Val Val Asp Ile Ala Leu Asn Tyr Ala Asp Thr Ala

370 375 380

Tyr Leu Val Ala Ala His Asn Arg His Ala Ser Ala Ala Asn Lys Ala

385 390 395 400

Leu Phe Leu Arg Leu Leu His Thr Ala Ile Pro Gln Val Glu Phe Ile

405 410 415

Lys Asp Ser Pro Ala Leu Ile Val Ala Arg Val Leu Ser Ser Leu Ile

420 425 430

Asn Glu Ser Val Ile Met Val Glu Ser Gly Val Cys Ser Arg Glu Asp

435 440 445

Ile Asp Val Ala Ala Val Ala Gly Val Asn Tyr Ala Gly Gly Ile Phe

450 455 460

Asp Trp Leu Ser Lys Leu Gly Glu Lys Asn Val Arg Thr Thr Leu Ser

465 470 475 480

Asn Leu Ala Gln Leu Leu His Ala Ala Arg Tyr Ala Pro His Tyr Thr

485 490 495

Leu Leu His Ala Ala Gln Pro Ala Leu Thr Thr Thr Pro

500 505

<210> 32

<211> 509

<212> PRT

<213> Serratia marcescens AH0650

<400> 32

Met Ala Glu Ser Asn Ala Ala Ile Gln Ser Ala Ala Ile Ile Gly Ala

1 5 10 15

Gly Thr Met Gly Arg Gly Ile Ala Tyr Leu Phe Ala Gln Lys Gly Ile

20 25 30

Arg Thr Val Leu Tyr Asn Arg Asn Gly Asn Thr Leu Asn Gln Ala Arg

35 40 45

Glu Tyr Ile Ala Gln Asp Leu Asn Lys Lys Val Glu Gln Gly Lys Ile

50 55 60

Ala Leu Gln Asp Lys Ser Ser Val Leu Ala Asn Leu Val Phe Ser Thr

65 70 75 80

Ala Leu Glu Ala Ile Ala Asp Ser Glu Leu Val Ile Glu Thr Ile Ala

85 90 95

Glu His Glu Gln Thr Lys Leu Glu Val Leu Ala Ala Ile Ala Ala Val

100 105 110

Val Lys Pro Asp Thr Leu Ile Ala Thr Asn Thr Ser Ser Leu Ser Leu

115 120 125

Asn Lys Leu Ala Thr Ala Val Thr His Ser Glu Arg Phe Ile Gly Leu

130 135 140

His Phe Phe Asn Pro Ala Pro Leu Met Lys Leu Ile Glu Ile Ile Pro

145 150 155 160

Ala Tyr Phe Thr Ala His Ala Thr Thr Glu Arg Cys Arg Gln Leu Val

165 170 175

Ala Ala Leu Gly Lys Arg Asp Val Val Cys Gln Ala Thr Pro Gly Phe

180 185 190

Ile Val Asn Arg Met Ala Arg Pro Tyr Tyr Leu Glu Gly Phe Arg Leu

195 200 205

Leu Glu Glu His Val Ala Arg Ala Pro Gln Ile Asp Arg Ala Leu Lys

210 215 220

Ala Gly Gly His Phe Arg Met Gly Pro Leu Glu Leu Thr Asp Phe Ile

225 230 235 240

Gly Gln Asp Ile Asn Tyr Gln Val Ser Arg Gln Ile Trp Gln Asp Met

245 250 255

Gln Tyr Asp Pro Arg Tyr Thr Pro Gly His Leu Gln Arg Ser Leu Val

260 265 270

Asp Ala Gly Leu Leu Gly Lys Lys Asn Gly Arg Ser Tyr Phe Ala Ala

275 280 285

Glu Glu Thr Ala Pro Pro Val Thr Ala Ala Ile Asp Ala Asp Val Glu

290 295 300

Thr Leu Arg Val Tyr Gly Glu His Pro Phe Phe Thr Leu Leu Gln Gln

305 310 315 320

Arg Ala Ala Arg Gln Trp Pro Gln Leu Arg Val Glu Gln Arg Pro Ala

325 330 335

Leu Pro Ser Leu Gly Pro Ala Val Gln Ile Asn Asp Ala Phe Thr Val

340 345 350

Ser Ile Thr Asp Gly Arg Thr Ala Ser Gln Leu Ala Glu Gln Thr Thr

355 360 365

Ala Asp Ala Phe Val Val Asp Ile Ala Leu Asn Tyr Ala Asp Thr Ala

370 375 380

Tyr Leu Val Ala Ala His Asn Arg Tyr Ala Ser Ala Ala Asn Lys Ala

385 390 395 400

Leu Phe Leu Arg Leu Leu His Thr Ala Ile Pro Gln Val Glu Phe Ile

405 410 415

Lys Asp Ser Pro Ala Leu Ile Val Ala Arg Val Leu Ser Ser Leu Ile

420 425 430

Asn Glu Ser Val Ile Met Val Glu Ser Gly Val Cys Ser Arg Glu Asp

435 440 445

Ile Asp Val Ala Ala Val Ala Gly Val Asn Tyr Ala Gly Gly Ile Phe

450 455 460

Asp Trp Leu Gly Lys Leu Gly Glu Lys Asn Val Arg Thr Thr Leu Ser

465 470 475 480

Asn Leu Ala Gln Leu Leu His Ala Ala Arg Tyr Ala Pro His Tyr Thr

485 490 495

Leu Leu His Ala Ala Gln Pro Ala Leu Thr Thr Thr Pro

500 505

<210> 33

<211> 509

<212> PRT

<213> Serratia marcescens UMH12

<400> 33

Met Ala Glu Ser Asn Ala Glu Ile Gln Ser Ala Ala Ile Ile Gly Ala

1 5 10 15

Gly Thr Met Gly Arg Gly Ile Ala Tyr Leu Phe Ala Gln Lys Gly Ile

20 25 30

Arg Thr Val Leu Tyr Asn Arg Asn Gly Asn Thr Leu Asn Gln Ala Arg

35 40 45

Glu Tyr Ile Ala Gln Asp Leu Asn Lys Lys Val Glu Gln Gly Lys Ile

50 55 60

Ala Leu Gln Asp Lys Ser Ser Val Leu Ala Asn Leu Val Phe Ser Thr

65 70 75 80

Ala Leu Glu Ala Ile Ala Asp Ser Glu Leu Val Ile Glu Thr Ile Ala

85 90 95

Glu His Glu Gln Thr Lys Leu Glu Val Leu Ala Ala Ile Ala Ala Val

100 105 110

Val Lys Pro Asp Thr Leu Ile Ala Thr Asn Thr Ser Ser Leu Ser Leu

115 120 125

Asn Lys Leu Ala Thr Ala Val Thr His Ser Glu Arg Phe Ile Gly Leu

130 135 140

His Phe Phe Asn Pro Ala Pro Leu Met Lys Leu Ile Glu Ile Ile Pro

145 150 155 160

Ala Tyr Phe Thr Ala His Ala Thr Thr Glu Arg Cys Arg Gln Leu Val

165 170 175

Ala Ala Leu Gly Lys Arg Asp Val Val Cys Gln Ala Thr Pro Gly Phe

180 185 190

Ile Val Asn Arg Met Ala Arg Pro Tyr Tyr Leu Glu Gly Phe Arg Leu

195 200 205

Leu Glu Glu His Val Ala Arg Ala Pro Gln Ile Asp Arg Ala Leu Lys

210 215 220

Ala Gly Gly His Phe Arg Met Gly Pro Leu Glu Leu Thr Asp Phe Ile

225 230 235 240

Gly Gln Asp Ile Asn Tyr Gln Val Ser Arg Gln Ile Trp Gln Asp Met

245 250 255

Gln Tyr Asp Pro Arg Tyr Thr Pro Gly His Leu Gln Arg Ser Leu Val

260 265 270

Asp Ala Gly Leu Leu Gly Lys Lys Asn Gly Arg Ser Tyr Phe Ala Ala

275 280 285

Glu Glu Thr Ala Pro Pro Val Thr Ala Ala Ile Asp Ala Asp Val Glu

290 295 300

Thr Leu Arg Val Tyr Gly Glu His Pro Phe Phe Thr Leu Leu Gln Gln

305 310 315 320

Arg Ala Ala Arg Gln Trp Pro Gln Leu Arg Val Glu Gln Arg Pro Ala

325 330 335

Leu Pro Ser Leu Gly Pro Ala Val Gln Ile Asn Asp Ala Phe Thr Val

340 345 350

Ser Ile Thr Asp Gly Arg Thr Ala Ser Gln Leu Ala Glu Gln Thr Thr

355 360 365

Ala Asp Ala Phe Val Val Asp Ile Ala Leu Asn Tyr Ala Asp Thr Ala

370 375 380

Tyr Leu Val Ala Ala His Asn Arg Tyr Ala Ser Ala Ala Asn Lys Ala

385 390 395 400

Leu Phe Leu Arg Leu Leu His Thr Ala Ile Pro Gln Val Glu Phe Ile

405 410 415

Lys Asp Ser Pro Ala Leu Ile Val Ala Arg Val Leu Ser Ser Leu Ile

420 425 430

Asn Glu Ser Val Ile Met Val Glu Ser Gly Val Cys Ser Arg Glu Asp

435 440 445

Ile Asp Val Ala Ala Val Ala Gly Val Asn Tyr Ala Gly Gly Ile Phe

450 455 460

Asp Trp Leu Gly Lys Leu Gly Glu Lys Asn Val Arg Thr Thr Leu Ser

465 470 475 480

Asn Leu Ala Gln Leu Leu His Ala Ala Arg Tyr Ala Pro His Tyr Thr

485 490 495

Leu Leu His Ala Ala Gln Pro Ala Leu Thr Thr Thr Pro

500 505

<210> 34

<211> 509

<212> PRT

<213> Serratia sp. OMLW3

<400> 34

Met Ala Glu Ser Asn Ala Ala Ile Gln Ser Ala Ala Ile Ile Gly Ala

1 5 10 15

Gly Thr Met Gly Arg Gly Ile Ala Tyr Leu Phe Ala Gln Lys Gly Ile

20 25 30

Arg Thr Val Leu Tyr Asn Arg Asn Gly Asn Thr Leu Asn Gln Ala Arg

35 40 45

Glu Tyr Ile Ala Gln Asp Leu Asn Lys Lys Val Glu Gln Gly Lys Ile

50 55 60

Ala Leu Gln Asp Lys Ser Ser Val Leu Ala Asn Leu Val Phe Ser Thr

65 70 75 80

Ala Leu Val Ala Ile Ala Asp Ser Glu Leu Val Ile Glu Thr Ile Ala

85 90 95

Glu His Glu Gln Thr Lys Leu Glu Val Leu Ala Ala Ile Ala Ala Val

100 105 110

Val Lys Pro Asp Thr Leu Ile Ala Thr Asn Thr Ser Ser Leu Ser Leu

115 120 125

Asn Lys Leu Ala Thr Ala Val Thr His Ser Glu Arg Phe Ile Gly Leu

130 135 140

His Phe Phe Asn Pro Ala Pro Leu Met Lys Leu Ile Glu Ile Ile Pro

145 150 155 160

Ala Tyr Phe Thr Ala His Ala Thr Thr Glu Arg Cys Arg Gln Leu Val

165 170 175

Ala Ala Leu Gly Lys Arg Asp Val Val Cys Gln Ala Thr Pro Gly Phe

180 185 190

Ile Val Asn Arg Met Ala Arg Pro Tyr Tyr Leu Glu Gly Phe Arg Leu

195 200 205

Leu Glu Glu His Val Ala Arg Ala Pro Gln Ile Asp Arg Ala Leu Lys

210 215 220

Ala Gly Gly His Phe Arg Met Gly Pro Leu Glu Leu Thr Asp Phe Ile

225 230 235 240

Gly Gln Asp Ile Asn Tyr Gln Val Ser Arg Gln Ile Trp Gln Asp Met

245 250 255

Gln Tyr Asp Pro Arg Tyr Thr Pro Gly His Leu Gln Arg Ser Leu Val

260 265 270

Asp Ala Gly Leu Leu Gly Lys Lys Asn Gly Arg Ser Tyr Phe Ala Ala

275 280 285

Glu Glu Thr Ala Pro Pro Val Thr Ala Ala Ile Asp Ala Asp Val Glu

290 295 300

Thr Leu Arg Val Tyr Gly Glu His Pro Phe Phe Thr Leu Leu Gln Gln

305 310 315 320

Arg Ala Ala Arg Gln Trp Pro Gln Leu Arg Val Glu Gln Arg Pro Ala

325 330 335

Leu Pro Ser Leu Gly Pro Ala Val Gln Ile Asn Asp Ala Phe Thr Val

340 345 350

Ser Ile Thr Asp Gly Arg Thr Ala Ser Gln Leu Ala Glu Gln Thr Thr

355 360 365

Ala Asp Ala Phe Val Val Asp Ile Ala Leu Asn Tyr Ala Asp Thr Ala

370 375 380

Tyr Leu Val Ala Ala His Asn Arg Tyr Ala Ser Ala Ala Asn Lys Ala

385 390 395 400

Leu Phe Leu Arg Leu Leu His Thr Ala Ile Pro Gln Val Glu Phe Ile

405 410 415

Lys Asp Ser Pro Ala Leu Ile Val Ala Arg Val Leu Ser Ser Leu Ile

420 425 430

Asn Glu Ser Val Ile Met Val Glu Ser Gly Val Cys Ser Arg Glu Asp

435 440 445

Ile Asp Val Ala Ala Val Ala Gly Val Asn Tyr Ala Gly Gly Ile Phe

450 455 460

Asp Trp Leu Gly Lys Leu Gly Glu Lys Asn Val Arg Thr Thr Leu Ser

465 470 475 480

Asn Leu Ala Gln Leu Leu His Ala Ala Arg Tyr Ala Pro His Tyr Thr

485 490 495

Leu Leu His Ala Ala Gln Pro Ala Leu Thr Thr Thr Pro

500 505

<210> 35

<211> 509

<212> PRT

<213> Serratia marcescens UMH11

<400> 35

Met Ala Glu Ser Asn Ala Ala Ile Gln Ser Ala Ala Ile Ile Gly Ala

1 5 10 15

Gly Thr Met Gly Arg Gly Ile Ala Tyr Leu Phe Ala Gln Lys Gly Ile

20 25 30

Arg Thr Val Leu Tyr Asn Arg Ser Gly Asn Thr Leu Asn Gln Ala Arg

35 40 45

Glu Tyr Ile Ala Gln Asp Leu Asn Lys Lys Val Glu Gln Gly Lys Ile

50 55 60

Ala Leu Gln Asp Lys Ser Ser Val Leu Ala Asn Leu Val Phe Ser Thr

65 70 75 80

Ala Leu Val Ala Ile Ala Asp Ser Glu Leu Val Ile Glu Thr Ile Ala

85 90 95

Glu His Glu Gln Thr Lys Leu Glu Val Leu Ala Ala Ile Ala Ala Val

100 105 110

Val Lys Pro Asp Thr Leu Ile Ala Thr Asn Thr Ser Ser Leu Ser Leu

115 120 125

Asn Lys Leu Ala Thr Ala Val Thr His Ser Glu Arg Phe Ile Gly Leu

130 135 140

His Phe Phe Asn Pro Ala Pro Leu Met Lys Leu Ile Glu Ile Ile Pro

145 150 155 160

Ala Tyr Phe Thr Ala His Ala Thr Thr Glu Arg Cys Arg Gln Leu Val

165 170 175

Ala Ala Leu Gly Lys Arg Asp Val Val Cys Gln Ala Thr Pro Gly Phe

180 185 190

Ile Val Asn Arg Met Ala Arg Pro Tyr Tyr Leu Glu Gly Phe Arg Leu

195 200 205

Leu Glu Glu His Val Ala Arg Ala Pro Gln Ile Asp Arg Ala Leu Lys

210 215 220

Ala Gly Gly His Phe Arg Met Gly Pro Leu Glu Leu Thr Asp Phe Ile

225 230 235 240

Gly Gln Asp Ile Asn Tyr Gln Val Ser Arg Gln Ile Trp Gln Asp Met

245 250 255

Gln Tyr Asp Pro Arg Tyr Thr Pro Gly His Leu Gln Arg Ser Leu Val

260 265 270

Asp Ala Gly Leu Leu Gly Lys Lys Asn Gly Arg Ser Tyr Phe Ala Ala

275 280 285

Glu Glu Thr Ala Pro Pro Val Thr Ala Ala Ile Asp Ala Asp Val Glu

290 295 300

Thr Leu Arg Val Tyr Gly Glu His Pro Phe Phe Thr Leu Leu Gln Gln

305 310 315 320

Arg Ala Ala Arg Gln Trp Pro Gln Leu Arg Val Glu Gln Arg Pro Ala

325 330 335

Leu Pro Ser Leu Gly Pro Ala Val Gln Ile Asn Asp Ala Phe Thr Val

340 345 350

Ser Ile Thr Asp Gly Arg Thr Ala Ser Gln Leu Ala Glu Gln Thr Thr

355 360 365

Ala Asp Ala Phe Val Val Asp Ile Ala Leu Asn Tyr Ala Asp Thr Ala

370 375 380

Tyr Leu Val Ala Ala His Asn Arg Tyr Ala Ser Ala Ala Asn Lys Ala

385 390 395 400

Leu Phe Leu Arg Leu Leu His Thr Ala Ile Pro Gln Val Glu Phe Ile

405 410 415

Lys Asp Ser Pro Ala Leu Ile Val Ala Arg Val Leu Ser Ser Leu Ile

420 425 430

Asn Glu Ser Val Ile Met Val Glu Ser Gly Val Cys Ser Arg Glu Asp

435 440 445

Ile Asp Val Ala Ala Val Ala Gly Val Asn Tyr Ala Gly Gly Ile Phe

450 455 460

Asp Trp Leu Gly Lys Leu Gly Glu Lys Asn Val Arg Thr Thr Leu Ser

465 470 475 480

Asn Leu Ala Gln Leu Leu His Ala Ala Arg Tyr Ala Pro His Tyr Thr

485 490 495

Leu Leu His Ala Ala Gln Pro Ala Leu Thr Thr Thr Pro

500 505

<210> 36

<211> 509

<212> PRT

<213> Serratia marcescens UMH1

<400> 36

Met Ala Glu Ser Asn Ala Ala Ile Gln Ser Ala Ala Ile Ile Gly Ala

1 5 10 15

Gly Thr Met Gly Arg Gly Ile Ala Tyr Leu Phe Ala Gln Lys Gly Ile

20 25 30

Arg Thr Val Leu Tyr Asn Arg Asn Gly Asn Thr Leu Asn Gln Ala Arg

35 40 45

Glu Tyr Ile Ala Gln Asp Leu Asn Lys Lys Val Glu Gln Gly Lys Ile

50 55 60

Ala Leu Gln Asp Lys Ser Ser Val Leu Ala Asn Leu Val Phe Ser Thr

65 70 75 80

Ala Leu Glu Ala Ile Ala Asp Ser Glu Leu Val Ile Glu Thr Ile Ala

85 90 95

Glu His Glu Gln Thr Lys Leu Glu Val Leu Ala Ala Ile Ala Ala Val

100 105 110

Val Lys Pro Asp Thr Leu Ile Ala Thr Asn Thr Ser Ser Leu Ser Leu

115 120 125

Asn Lys Leu Ala Thr Ala Val Thr His Ser Glu Arg Phe Ile Gly Leu

130 135 140

His Phe Phe Asn Pro Ala Pro Leu Met Lys Leu Ile Glu Ile Ile Pro

145 150 155 160

Ala Tyr Phe Thr Ala His Ala Thr Thr Glu Arg Cys Arg Gln Leu Val

165 170 175

Ala Ala Leu Gly Lys Arg Asp Val Val Cys Gln Ala Thr Pro Gly Phe

180 185 190

Ile Val Asn Arg Met Ala Arg Pro Tyr Tyr Leu Glu Gly Phe Arg Leu

195 200 205

Leu Glu Glu His Val Ala Arg Ala Pro Gln Ile Asp Arg Ala Leu Lys

210 215 220

Ala Gly Gly His Phe Arg Met Gly Pro Leu Glu Leu Thr Asp Phe Ile

225 230 235 240

Gly Gln Asp Ile Asn Tyr Gln Val Ser Arg Gln Ile Trp Gln Asp Met

245 250 255

Gln Tyr Asp Pro Arg Tyr Thr Pro Gly His Leu Gln Arg Ser Leu Val

260 265 270

Asp Ala Gly Leu Leu Gly Lys Lys Asn Gly Arg Ser Tyr Phe Ala Ala

275 280 285

Glu Glu Thr Ala Pro Pro Val Thr Ala Ala Ile Asp Ala Asp Val Glu

290 295 300

Thr Leu Arg Val Tyr Gly Glu His Pro Phe Phe Thr Leu Leu Gln Gln

305 310 315 320

Gln Ala Ala Arg Gln Trp Pro Gln Leu Arg Val Glu Gln Arg Pro Ala

325 330 335

Leu Pro Ser Leu Gly Pro Ala Val Gln Ile Asn Gly Ala Phe Thr Val

340 345 350

Ser Ile Thr Asp Gly Arg Thr Ala Ser Gln Leu Ala Glu Gln Thr Thr

355 360 365

Ala Asp Ala Phe Val Ile Asp Ile Ala Leu Asn Tyr Ala Asp Thr Ala

370 375 380

Tyr Leu Val Ala Ala His Asn Arg Tyr Ala Ser Ala Ala Asn Lys Ala

385 390 395 400

Leu Phe Leu Arg Leu Leu His Thr Ala Ile Pro Gln Val Glu Phe Ile

405 410 415

Lys Asp Ser Pro Ala Leu Ile Val Ala Arg Val Leu Ser Ser Leu Ile

420 425 430

Asn Glu Ser Val Ile Met Val Glu Ser Gly Val Cys Ser Arg Glu Asp

435 440 445

Ile Asp Val Ala Ala Val Ala Gly Val Asn Tyr Ala Gly Gly Ile Phe

450 455 460

Asp Trp Leu Gly Lys Leu Gly Glu Lys Asn Val Arg Thr Thr Leu Ser

465 470 475 480

Asn Leu Ala Gln Leu Leu His Ala Ala Arg Tyr Ala Pro His Tyr Thr

485 490 495

Leu Leu His Ala Ala Gln Pro Ala Leu Thr Thr Thr Pro

500 505

<210> 37

<211> 509

<212> PRT

<213> Serratia marcescens 2880STDY5683020

<400> 37

Met Ala Glu Ser Asn Ala Ala Ile Gln Ser Ala Ala Ile Ile Gly Ala

1 5 10 15

Gly Thr Met Gly Arg Gly Ile Ala Tyr Leu Phe Ala Gln Lys Gly Ile

20 25 30

Arg Thr Val Leu Tyr Asn Arg Asn Gly Asn Thr Leu Asn Gln Ala Arg

35 40 45

Glu Tyr Ile Ala Gln Asp Leu Asn Lys Lys Val Glu Gln Gly Lys Ile

50 55 60

Ala Leu Gln Asp Lys Gly Ala Val Leu Ala Asn Leu Val Phe Thr Ser

65 70 75 80

Val Phe Glu Thr Ile Ala Asp Ser Asp Leu Val Ile Glu Thr Ile Ala

85 90 95

Glu Gln Glu Gln Thr Lys Leu Glu Val Leu Ala Ala Ile Ala Ala Ala

100 105 110

Val Lys Pro Asp Thr Leu Ile Ala Thr Asn Thr Ser Ser Leu Ser Leu

115 120 125

Asn Lys Leu Ala Thr Ala Val Thr His Ser Glu Arg Phe Ile Gly Leu

130 135 140

His Phe Phe Asn Pro Ala Pro Leu Met Lys Leu Ile Glu Ile Ile Pro

145 150 155 160

Ala Tyr Phe Thr Ala Arg Ala Thr Thr Glu Arg Cys Arg Gln Leu Val

165 170 175

Ala Ala Leu Gly Lys Arg Asp Val Val Cys Gln Ala Thr Pro Gly Phe

180 185 190

Ile Val Asn Arg Met Ala Arg Pro Tyr Tyr Leu Glu Gly Phe Arg Leu

195 200 205

Leu Glu Glu His Val Ala Arg Ala Pro Gln Ile Asp Arg Ala Leu Lys

210 215 220

Ala Gly Gly His Phe Arg Met Gly Pro Leu Glu Leu Thr Asp Phe Ile

225 230 235 240

Gly Gln Asp Ile Asn Tyr Gln Val Ser Arg Gln Ile Trp Gln Asp Met

245 250 255

Gln Tyr Asp Pro Arg Tyr Thr Pro Gly His Leu Gln Arg Ser Leu Val

260 265 270

Asp Ala Gly Leu Leu Gly Lys Lys Asn Gly Arg Ser Tyr Phe Ala Ala

275 280 285

Glu Glu Asn Ala Leu Pro Val Thr Ala Ala Thr Asp Ala Asp Val Glu

290 295 300

Thr Leu Arg Val Tyr Gly Glu His Pro Phe Phe Thr Leu Leu Gln Gln

305 310 315 320

Arg Ala Ala Leu Gln Trp Pro Arg Leu Arg Val Glu Gln Arg Pro Ala

325 330 335

Leu Pro Gly Leu Gly Pro Ala Val Gln Ile Asn Glu Ala Phe Thr Val

340 345 350

Ser Val Thr Asp Gly Arg Thr Ala Ser Gln Leu Ala Glu Gln Thr Ala

355 360 365

Ala Asp Ala Phe Val Val Asp Val Ala Leu Asn Tyr Ala Asp Thr Ala

370 375 380

Tyr Leu Val Ala Ala His Asn Arg His Ala Ser Ala Ala Asn Lys Ala

385 390 395 400

Leu Phe Leu Arg Leu Leu His Thr Ala Ile Pro Gln Val Glu Phe Ile

405 410 415

Lys Asp Ser Pro Ala Leu Ile Val Ala Arg Val Leu Ser Ser Leu Ile

420 425 430

Asn Glu Ser Val Ile Met Val Glu Ser Gly Val Cys Ser Arg Glu Asp

435 440 445

Ile Asp Val Ala Ala Val Ala Gly Val Asn Tyr Ala Asp Gly Ile Phe

450 455 460

Gly Trp Leu Asp Ser Leu Gly Glu Lys Asn Val Arg Thr Thr Leu Ser

465 470 475 480

Asn Leu Ala Gln Leu Leu His Ala Ala Arg Tyr Ala Pro His Tyr Thr

485 490 495

Leu Leu His Ala Ala Gln Pro Ala Leu Thr Thr Thr Pro

500 505

<210> 38

<211> 509

<212> PRT

<213> Serratia marcescens 99

<400> 38

Met Ala Glu Ser Asn Ala Ala Ile Gln Ser Ala Ala Ile Ile Gly Ala

1 5 10 15

Gly Thr Met Gly Arg Gly Ile Ala Tyr Leu Phe Ala Gln Lys Gly Ile

20 25 30

Arg Thr Val Leu Tyr Asn Arg Asn Gly Asn Thr Leu Asn Gln Ala Arg

35 40 45

Glu Tyr Ile Ala Gln Asp Leu Asn Lys Lys Val Glu Gln Gly Lys Ile

50 55 60

Ala Leu Gln Asp Lys Gly Ala Val Leu Ala Asn Leu Val Phe Thr Ser

65 70 75 80

Val Phe Glu Thr Ile Ala Asp Ser Asp Leu Val Ile Glu Thr Ile Ala

85 90 95

Glu Gln Glu Gln Thr Lys Leu Glu Val Leu Ala Ala Ile Ala Ala Ala

100 105 110

Val Lys Pro Asp Thr Leu Ile Ala Thr Asn Thr Ser Ser Leu Ser Leu

115 120 125

Asn Lys Leu Ala Thr Ala Val Thr His Ser Glu Arg Phe Ile Gly Leu

130 135 140

His Phe Phe Asn Pro Ala Pro Leu Met Lys Leu Ile Glu Ile Ile Pro

145 150 155 160

Ala Tyr Phe Thr Ala Arg Ala Thr Thr Glu Arg Cys Arg Gln Leu Val

165 170 175

Ala Ala Leu Gly Lys Arg Asp Val Val Cys Gln Ala Thr Pro Gly Phe

180 185 190

Ile Val Asn Arg Met Ala Arg Pro Tyr Tyr Leu Glu Gly Phe Arg Leu

195 200 205

Leu Glu Glu His Val Ala Arg Ala Pro Gln Ile Asp Arg Ala Leu Lys

210 215 220

Ala Gly Gly His Phe Arg Met Gly Pro Leu Glu Leu Thr Asp Phe Ile

225 230 235 240

Gly Gln Asp Ile Asn Tyr Gln Val Ser Arg Gln Ile Trp Gln Asp Met

245 250 255

Gln Tyr Asp Pro Arg Tyr Thr Pro Gly His Leu Gln Arg Ser Leu Val

260 265 270

Asp Ala Gly Leu Leu Gly Lys Lys Asn Gly Arg Ser Tyr Phe Ala Ala

275 280 285

Glu Glu Asn Ala Leu Pro Val Thr Ala Ala Ser Asn Ala Asp Val Glu

290 295 300

Thr Leu Arg Val Tyr Gly Glu His Pro Phe Phe Thr Leu Leu Gln Gln

305 310 315 320

Arg Ala Ala Leu Gln Trp Pro Arg Leu Arg Val Glu Gln Arg Pro Ala

325 330 335

Leu Pro Gly Leu Gly Pro Ala Val Gln Ile Asn Glu Ala Phe Thr Val

340 345 350

Ser Val Thr Asp Gly Arg Thr Ala Ser Gln Leu Ala Glu Leu Thr Ala

355 360 365

Ala Asp Ala Phe Val Val Asp Val Ala Leu Asn Tyr Ala Asp Thr Ala

370 375 380

Tyr Leu Val Ala Ala His Asn Arg His Ala Ser Ala Ala Asn Lys Ala

385 390 395 400

Leu Phe Leu Arg Leu Leu His Thr Ala Ile Pro Gln Val Glu Phe Ile

405 410 415

Lys Asp Ser Pro Ala Leu Ile Val Ala Arg Val Leu Ser Ser Leu Ile

420 425 430

Asn Glu Ser Val Ile Met Val Glu Ser Gly Val Cys Ser Arg Glu Asp

435 440 445

Ile Asp Val Ala Ala Val Ala Gly Val Asn Tyr Ala Asp Gly Ile Phe

450 455 460

Gly Trp Leu Asp Ser Leu Gly Glu Lys Asn Val Arg Thr Thr Leu Ser

465 470 475 480

Asn Leu Ala Gln Leu Leu His Ala Ala Arg Tyr Ala Pro His Tyr Thr

485 490 495

Leu Leu His Ala Ala Gln Pro Val Leu Thr Thr Thr Pro

500 505

<210> 39

<211> 509

<212> PRT

<213> Serratia marcescens 374

<400> 39

Met Ala Glu Ser Asn Ala Ala Ile Gln Ser Ala Ala Ile Ile Gly Ala

1 5 10 15

Gly Thr Met Gly Arg Gly Ile Ala Tyr Leu Phe Ala Gln Lys Gly Ile

20 25 30

Arg Thr Val Leu Tyr Asn Arg Asn Gly Asn Thr Leu Asn Gln Ala Arg

35 40 45

Glu Tyr Ile Ala Gln Asp Leu Asn Lys Lys Val Glu Gln Gly Lys Ile

50 55 60

Ala Leu Gln Asp Lys Ser Ser Val Leu Ala Asn Leu Val Phe Ser Thr

65 70 75 80

Ala Leu Val Ala Ile Ala Asp Ser Glu Leu Val Ile Glu Thr Ile Ala

85 90 95

Glu His Glu Gln Thr Lys Leu Glu Val Leu Ala Ala Ile Ala Ala Val

100 105 110

Val Lys Pro Asp Thr Leu Ile Ala Thr Asn Thr Ser Ser Leu Ser Leu

115 120 125

Asn Lys Leu Ala Thr Ala Val Thr His Ser Glu Arg Phe Ile Gly Leu

130 135 140

His Phe Phe Asn Pro Ala Pro Leu Met Lys Leu Ile Glu Ile Ile Pro

145 150 155 160

Ala Tyr Phe Thr Ala His Ala Thr Thr Glu Arg Cys Arg Gln Leu Val

165 170 175

Ala Ala Leu Gly Lys Arg Asp Val Val Cys Gln Ala Thr Pro Gly Phe

180 185 190

Ile Val Asn Arg Met Ala Arg Pro Tyr Tyr Leu Glu Gly Phe Arg Leu

195 200 205

Leu Glu Glu His Val Ala Arg Ala Pro Gln Ile Asp Arg Ala Leu Lys

210 215 220

Ala Gly Gly His Phe Arg Met Gly Pro Leu Glu Leu Thr Asp Phe Ile

225 230 235 240

Gly Gln Asp Ile Asn Tyr Gln Val Ser Arg Gln Ile Trp Gln Asp Met

245 250 255

Gln Tyr Asp Pro Arg Tyr Thr Pro Gly His Leu Gln Arg Ser Leu Val

260 265 270

Asp Ala Gly Leu Leu Gly Lys Lys Asn Gly Arg Ser Tyr Phe Ala Ala

275 280 285

Glu Glu Thr Ala Pro Pro Val Thr Ala Ala Ile Asp Ala Asp Val Glu

290 295 300

Thr Leu Arg Val Tyr Gly Glu His Pro Phe Phe Thr Leu Leu Gln Gln

305 310 315 320

Gln Ala Ala Arg Gln Trp Pro Gln Leu Arg Val Glu Gln Arg Pro Ala

325 330 335

Leu Pro Ser Leu Gly Pro Ala Val Gln Ile Asn Gly Ala Phe Thr Val

340 345 350

Ser Ile Thr Asp Gly Arg Thr Ala Ser Gln Leu Ala Glu Gln Thr Thr

355 360 365

Ala Asp Ala Phe Val Ile Asp Ile Ala Leu Asn Tyr Ala Asp Thr Ala

370 375 380

Tyr Leu Val Ala Ala His Asn Arg Tyr Ala Ser Ala Ala Asn Lys Ala

385 390 395 400

Leu Phe Leu Arg Leu Leu His Thr Ala Ile Pro Gln Val Glu Phe Ile

405 410 415

Lys Asp Ser Pro Ala Leu Ile Val Ala Arg Val Leu Ser Ser Leu Ile

420 425 430

Asn Glu Ser Val Ile Met Val Glu Ser Gly Val Cys Ser Arg Glu Asp

435 440 445

Ile Asp Val Ala Ala Val Ala Gly Val Asn Tyr Ala Gly Gly Ile Phe

450 455 460

Asp Trp Leu Gly Lys Leu Gly Glu Lys Asn Val Arg Thr Thr Leu Ser

465 470 475 480

Asn Leu Ala Gln Leu Leu His Ala Ala Arg Tyr Ala Pro His Tyr Thr

485 490 495

Leu Leu His Ala Ala Gln Pro Ala Leu Thr Thr Thr Pro

500 505

<210> 40

<211> 509

<212> PRT

<213> Serratia marcescens 2880STDY5683036

<400> 40

Met Ala Glu Ser Asn Ala Ala Ile Gln Ser Ala Ala Ile Ile Gly Ala

1 5 10 15

Gly Thr Met Gly Arg Gly Ile Ala Tyr Leu Phe Ala Gln Lys Gly Ile

20 25 30

Arg Thr Val Leu Tyr Asn Arg Asn Gly Asn Thr Leu Asn Gln Ala Arg

35 40 45

Glu Tyr Ile Ala Gln Asp Leu Asn Lys Lys Val Glu Gln Gly Lys Ile

50 55 60

Ala Leu Gln Asp Lys Gly Ala Val Leu Ala Asn Leu Val Phe Thr Ser

65 70 75 80

Val Phe Glu Thr Ile Ala Asp Ser Asp Leu Val Ile Glu Thr Ile Ala

85 90 95

Glu Gln Glu Gln Thr Lys Leu Glu Val Leu Ala Ala Ile Ala Ala Ala

100 105 110

Val Lys Pro Asp Thr Leu Leu Ala Thr Asn Thr Ser Ser Leu Ser Leu

115 120 125

Asn Lys Leu Ala Thr Ala Val Thr His Ser Glu Arg Phe Ile Gly Leu

130 135 140

His Phe Phe Asn Pro Ala Pro Leu Met Lys Leu Ile Glu Ile Ile Pro

145 150 155 160

Ala Tyr Phe Thr Ala Arg Ala Thr Thr Glu Arg Cys Arg Gln Leu Val

165 170 175

Ala Ala Leu Gly Lys Arg Asp Val Val Cys Gln Ala Thr Pro Gly Phe

180 185 190

Ile Val Asn Arg Met Ala Arg Pro Tyr Tyr Leu Glu Gly Phe Arg Leu

195 200 205

Leu Glu Glu His Val Ala Arg Ala Pro Gln Ile Asp Arg Ala Leu Lys

210 215 220

Ala Gly Gly His Phe Arg Met Gly Pro Leu Glu Leu Thr Asp Phe Ile

225 230 235 240

Gly Gln Asp Ile Asn Tyr Gln Val Ser Arg Gln Ile Trp Gln Asp Met

245 250 255

Gln Tyr Asp Pro Arg Tyr Thr Pro Gly His Leu Gln Arg Ser Leu Val

260 265 270

Asp Ala Gly Leu Leu Gly Lys Lys Asn Gly Arg Ser Tyr Phe Ala Ala

275 280 285

Glu Glu Asn Ala Leu Pro Val Thr Ala Ala Ser Asn Ala Asp Val Glu

290 295 300

Thr Leu Arg Val Tyr Gly Glu His Pro Phe Phe Thr Leu Leu Gln Gln

305 310 315 320

Arg Ala Ala Leu Gln Trp Pro Arg Leu Arg Val Glu Gln Arg Pro Ala

325 330 335

Leu Pro Gly Leu Gly Pro Ala Val Gln Ile Asn Glu Ala Phe Thr Val

340 345 350

Ser Val Thr Asp Gly Arg Thr Ala Ser Gln Leu Ala Glu Leu Thr Ala

355 360 365

Ala Asp Ala Phe Val Val Asp Val Ala Leu Asn Tyr Ala Asp Thr Ala

370 375 380

Tyr Leu Val Ala Ala His Asn Arg His Ala Ser Ala Ala Asn Lys Ala

385 390 395 400

Leu Phe Leu Arg Leu Leu His Thr Ala Ile Pro Gln Val Glu Phe Ile

405 410 415

Lys Asp Ser Pro Ala Leu Ile Val Ala Arg Val Leu Ser Ser Leu Ile

420 425 430

Asn Glu Ser Val Ile Met Val Glu Ser Gly Val Cys Ser Arg Glu Asp

435 440 445

Ile Asp Val Ala Ala Val Ala Gly Val Asn Tyr Ala Asp Gly Ile Phe

450 455 460

Gly Trp Leu Asp Ser Leu Gly Glu Lys Asn Val Arg Thr Thr Leu Ser

465 470 475 480

Asn Leu Ala Gln Leu Leu His Ala Ala Arg Tyr Ala Pro His Tyr Thr

485 490 495

Leu Leu His Ala Ala Gln Pro Val Leu Thr Thr Thr Pro

500 505

<210> 41

<211> 509

<212> PRT

<213> Serratia marcescens 2880STDY5683034

<400> 41

Met Ala Glu Ser Asn Ala Ala Ile Gln Ser Ala Ala Ile Ile Gly Ala

1 5 10 15

Gly Thr Met Gly Arg Gly Ile Ala Tyr Leu Phe Ala Gln Lys Gly Ile

20 25 30

Arg Thr Val Leu Tyr Asn Arg Asn Gly Asn Thr Leu Asn Gln Ala Arg

35 40 45

Glu Tyr Ile Ala Gln Asp Leu Asn Lys Lys Val Glu Gln Gly Lys Ile

50 55 60

Ala Leu Gln Asp Lys Gly Ala Val Leu Ala Asn Leu Val Phe Thr Ser

65 70 75 80

Val Phe Glu Thr Ile Ala Asp Ser Glu Leu Val Ile Glu Thr Ile Ala

85 90 95

Glu Gln Glu Gln Thr Lys Leu Glu Val Leu Ala Ala Ile Ala Ala Ala

100 105 110

Val Lys Pro Asp Thr Leu Ile Ala Thr Asn Thr Ser Ser Leu Ser Leu

115 120 125

Asn Lys Leu Ala Thr Ala Val Thr His Ser Glu Arg Phe Ile Gly Leu

130 135 140

His Phe Phe Asn Pro Ala Pro Leu Met Lys Leu Ile Glu Ile Ile Pro

145 150 155 160

Ala Tyr Phe Thr Ala Arg Ala Thr Thr Glu Arg Cys Arg Gln Leu Val

165 170 175

Ala Ala Leu Gly Lys Arg Asp Val Val Cys Gln Ala Thr Pro Gly Phe

180 185 190

Ile Val Asn Arg Met Ala Arg Pro Tyr Tyr Leu Glu Gly Phe Arg Leu

195 200 205

Leu Glu Glu His Val Ala Arg Ala Pro Gln Ile Asp Arg Ala Leu Lys

210 215 220

Ala Gly Gly His Phe Arg Met Gly Pro Leu Glu Leu Thr Asp Phe Ile

225 230 235 240

Gly Gln Asp Ile Asn Tyr Gln Val Ser Arg Gln Ile Trp Gln Asp Met

245 250 255

Gln Tyr Asp Pro Arg Tyr Thr Pro Gly His Leu Gln Arg Ser Leu Val

260 265 270

Asp Ala Gly Leu Leu Gly Lys Lys Asn Gly Arg Ser Tyr Phe Ala Ala

275 280 285

Glu Glu Asn Ala Leu Pro Val Thr Ala Ala Thr Asp Ala Asp Val Glu

290 295 300

Thr Leu Arg Ile Tyr Gly Glu His Pro Phe Phe Thr Leu Leu Gln Gln

305 310 315 320

Arg Ala Ala Leu Gln Trp Pro Arg Leu Arg Val Glu Gln Arg Pro Ala

325 330 335

Leu Pro Gly Leu Gly Pro Ala Val Gln Ile Asn Glu Ala Phe Thr Val

340 345 350

Ser Val Thr Asp Gly Arg Thr Ala Ser Gln Leu Ala Glu Leu Thr Ala

355 360 365

Ala Asp Ala Phe Val Val Asp Val Ala Leu Asn Tyr Ala Asp Thr Ala

370 375 380

Tyr Leu Val Ala Ala His Asn Arg His Ala Ser Ala Ala Asn Lys Ala

385 390 395 400

Leu Phe Leu Arg Leu Leu His Thr Ala Ile Pro Gln Val Glu Phe Ile

405 410 415

Lys Asp Ser Pro Ala Leu Ile Val Ala Arg Val Leu Ser Ser Leu Ile

420 425 430

Asn Glu Ser Val Ile Met Val Glu Ser Gly Val Cys Ser Arg Glu Asp

435 440 445

Ile Asp Val Ala Ala Val Ala Gly Val Asn Tyr Ala Asp Gly Ile Phe

450 455 460

Gly Trp Leu Asp Asn Leu Gly Glu Lys Asn Val Arg Thr Thr Leu Ser

465 470 475 480

Asn Leu Ala Gln Leu Leu His Ala Ala Arg Tyr Ala Pro His Tyr Thr

485 490 495

Leu Leu His Ala Ala Gln Pro Ala Leu Thr Thr Thr Pro

500 505

<210> 42

<211> 509

<212> PRT

<213> Serratia marcescens 2880STDY5682892

<400> 42

Met Ala Glu Ser Asn Ala Ala Ile Gln Ser Ala Ala Ile Ile Gly Ala

1 5 10 15

Gly Thr Met Gly Arg Gly Ile Ala Tyr Leu Phe Ala Gln Lys Gly Ile

20 25 30

Arg Thr Val Leu Tyr Asn Arg Asn Gly Asn Thr Leu Asn Gln Ala Arg

35 40 45

Glu Tyr Ile Ala Gln Asp Leu Asn Lys Lys Val Glu Gln Gly Lys Ile

50 55 60

Ala Leu Gln Asp Lys Gly Ala Val Leu Ala Asn Leu Val Phe Thr Ser

65 70 75 80

Val Phe Glu Thr Ile Ala Asp Ser Asp Leu Val Ile Glu Thr Ile Ala

85 90 95

Glu Gln Glu Gln Thr Lys Leu Glu Val Leu Ala Ala Ile Ala Ala Ala

100 105 110

Val Lys Pro Asp Thr Leu Ile Ala Thr Asn Thr Ser Ser Leu Ser Leu

115 120 125

Asn Lys Leu Ala Thr Ala Val Thr His Ser Glu Arg Phe Ile Gly Leu

130 135 140

His Phe Phe Asn Pro Ala Pro Leu Met Lys Leu Ile Glu Ile Ile Pro

145 150 155 160

Ala Tyr Phe Thr Ala Arg Ala Thr Thr Glu Arg Cys Arg Gln Leu Val

165 170 175

Ala Ala Leu Gly Lys Arg Asp Val Val Cys Gln Ala Thr Pro Gly Phe

180 185 190

Ile Val Asn Arg Met Ala Arg Pro Tyr Tyr Leu Glu Gly Phe Arg Leu

195 200 205

Leu Glu Glu His Val Ala Arg Ala Pro Gln Ile Asp Arg Ala Leu Lys

210 215 220

Ala Gly Gly His Phe Arg Met Gly Pro Leu Glu Leu Thr Asp Phe Ile

225 230 235 240

Gly Gln Asp Ile Asn Tyr Gln Val Ser Arg Gln Ile Trp Gln Asp Met

245 250 255

Gln Tyr Asp Pro Arg Tyr Thr Pro Gly His Leu Gln Arg Ser Leu Val

260 265 270

Asp Ala Gly Leu Leu Gly Lys Lys Asn Gly Arg Ser Tyr Phe Ala Ala

275 280 285

Glu Glu Asn Ala Leu Pro Val Thr Ala Ala Thr Asp Ala Asp Val Glu

290 295 300

Thr Leu Arg Val Tyr Gly Glu His Pro Phe Phe Thr Leu Leu Gln Gln

305 310 315 320

Arg Ala Ala Leu Gln Trp Pro Arg Leu Arg Val Glu Gln Arg Pro Ala

325 330 335

Leu Pro Gly Leu Gly Pro Ala Val Gln Ile Asn Glu Ala Phe Thr Val

340 345 350

Ser Val Thr Asp Gly Arg Thr Ala Ser Gln Leu Ala Glu Leu Thr Ala

355 360 365

Ala Asp Ala Phe Val Val Asp Val Ala Leu Asn Tyr Ala Asp Thr Ala

370 375 380

Tyr Leu Val Ala Ala His Asn Arg His Ala Ser Ala Ala Asn Lys Ala

385 390 395 400

Leu Phe Leu Arg Leu Leu His Thr Ala Ile Pro Gln Val Glu Phe Ile

405 410 415

Lys Asp Ser Pro Ala Leu Ile Val Ala Arg Val Leu Ser Ser Leu Ile

420 425 430

Asn Glu Ser Val Ile Met Val Glu Ser Gly Val Cys Ser Arg Glu Asp

435 440 445

Ile Asp Val Ala Ala Val Ala Gly Val Asn Tyr Ala Asp Gly Ile Phe

450 455 460

Gly Trp Leu Asp Ser Leu Gly Glu Lys Asn Val Arg Thr Thr Leu Ser

465 470 475 480

Asn Leu Ala Gln Leu Leu His Ala Ala Arg Tyr Ala Pro His Tyr Thr

485 490 495

Leu Leu His Ala Ala Gln Pro Ala Leu Thr Thr Thr Pro

500 505

<210> 43

<211> 509

<212> PRT

<213> Serratia marcescens SM39

<400> 43

Met Ala Glu Ser Asn Ala Ala Ile Gln Ser Ala Ala Ile Ile Gly Ala

1 5 10 15

Gly Thr Met Gly Arg Gly Ile Ala Tyr Leu Phe Ala Gln Lys Gly Ile

20 25 30

Arg Thr Val Leu Tyr Asn Arg Asn Gly Asn Thr Leu Asn Gln Ala Arg

35 40 45

Glu Tyr Ile Val Gln Asp Leu Asn Lys Lys Val Glu Gln Gly Lys Ile

50 55 60

Ala Leu Gln Asp Lys Gly Ala Val Leu Ala Asn Leu Val Phe Thr Ser

65 70 75 80

Val Phe Glu Thr Ile Ala Asp Ser Glu Leu Val Ile Glu Thr Ile Ala

85 90 95

Glu Gln Glu Gln Thr Lys Leu Glu Val Leu Ala Ala Ile Ala Ala Ala

100 105 110

Val Lys Pro Asp Thr Leu Ile Ala Thr Asn Thr Ser Ser Leu Ser Leu

115 120 125

Asn Lys Leu Ala Thr Ala Val Thr His Ser Glu Arg Phe Ile Gly Leu

130 135 140

His Phe Phe Asn Pro Ala Pro Leu Met Lys Leu Ile Glu Ile Ile Pro

145 150 155 160

Ala Tyr Phe Thr Ala Arg Ala Thr Thr Glu Arg Cys Arg Gln Leu Val

165 170 175

Ala Ala Leu Gly Lys Arg Asp Val Val Cys Gln Ala Thr Pro Gly Phe

180 185 190

Ile Val Asn Arg Met Ala Arg Pro Tyr Tyr Leu Glu Gly Phe Arg Leu

195 200 205

Leu Glu Glu His Val Ala Arg Ala Pro Gln Ile Asp Arg Ala Leu Lys

210 215 220

Ala Gly Gly His Phe Arg Met Gly Pro Leu Glu Leu Thr Asp Phe Ile

225 230 235 240

Gly Gln Asp Ile Asn Tyr Gln Val Ser Arg Gln Ile Trp Gln Asp Met

245 250 255

Gln Tyr Asp Pro Arg Tyr Thr Pro Gly His Leu Gln Arg Ser Leu Val

260 265 270

Asp Ala Gly Leu Leu Gly Lys Lys Asn Gly Arg Ser Tyr Phe Ala Ala

275 280 285

Glu Glu Asn Ala Leu Pro Val Thr Ala Ala Thr Asp Ala Asp Val Glu

290 295 300

Thr Leu Arg Ile Tyr Gly Glu His Pro Phe Phe Thr Leu Leu Gln Gln

305 310 315 320

Arg Ala Ala Leu Gln Trp Pro Arg Leu Arg Val Glu Gln Arg Pro Ala

325 330 335

Leu Pro Gly Leu Gly Pro Ala Val Gln Ile Asn Glu Ala Phe Thr Val

340 345 350

Ser Val Thr Asp Gly Arg Thr Ala Ser Gln Leu Ala Glu Leu Thr Ala

355 360 365

Ala Asp Ala Phe Val Val Asp Val Ala Leu Asn Tyr Ala Asp Thr Ala

370 375 380

Tyr Leu Val Ala Ala His Asn Arg His Ala Ser Ala Ala Asn Lys Ala

385 390 395 400

Leu Phe Leu Arg Leu Leu His Thr Ala Ile Pro Gln Val Glu Phe Ile

405 410 415

Lys Asp Ser Pro Ala Leu Ile Val Ala Arg Val Leu Ser Ser Leu Ile

420 425 430

Asn Glu Ser Val Ile Met Val Glu Ser Gly Val Cys Ser Arg Glu Asp

435 440 445

Ile Asp Val Ala Ala Val Ala Gly Val Asn Tyr Ala Asp Gly Ile Phe

450 455 460

Gly Trp Leu Asp Asn Leu Gly Glu Lys Asn Val Arg Thr Thr Leu Ser

465 470 475 480

Asn Leu Ala Gln Leu Leu His Ala Ala Arg Tyr Ala Pro His Tyr Thr

485 490 495

Leu Leu His Ala Ala Gln Pro Ala Leu Thr Thr Thr Pro

500 505

<210> 44

<211> 509

<212> PRT

<213> Serratia marcescens 189

<400> 44

Met Ala Glu Ser Asn Ala Ala Ile Gln Ser Ala Ala Ile Ile Gly Ala

1 5 10 15

Gly Thr Met Gly Arg Gly Ile Ala Tyr Leu Phe Ala Gln Lys Gly Ile

20 25 30

Arg Thr Val Leu Tyr Asn Arg Asn Gly Asn Thr Leu Asn Gln Ala Arg

35 40 45

Glu Tyr Ile Ala Gln Asp Leu Asn Lys Lys Val Glu Gln Gly Lys Ile

50 55 60

Ala Leu Gln Asp Lys Gly Ala Val Leu Ala Asn Leu Val Phe Thr Ser

65 70 75 80

Val Phe Glu Thr Ile Ala Asp Ser Asp Leu Val Ile Glu Thr Ile Ala

85 90 95

Glu Gln Glu Gln Thr Lys Leu Glu Val Leu Ala Ala Ile Ala Ala Ala

100 105 110

Val Lys Pro Asp Thr Leu Ile Ala Thr Asn Thr Ser Ser Leu Ser Leu

115 120 125

Asn Lys Leu Ala Thr Ala Val Thr His Ser Glu Arg Phe Ile Gly Leu

130 135 140

His Phe Phe Asn Pro Ala Pro Leu Met Lys Leu Ile Glu Ile Ile Pro

145 150 155 160

Ala Tyr Phe Thr Ala Arg Ala Thr Thr Glu Arg Cys Arg Gln Leu Val

165 170 175

Ala Ala Leu Gly Lys Arg Asp Val Val Cys Gln Ala Thr Pro Gly Phe

180 185 190

Ile Val Asn Arg Met Ala Arg Pro Tyr Tyr Leu Glu Gly Phe Arg Leu

195 200 205

Leu Glu Glu His Val Ala Arg Ala Pro Gln Ile Asp Arg Ala Leu Lys

210 215 220

Ala Gly Gly His Phe Arg Met Gly Pro Leu Glu Leu Thr Asp Phe Ile

225 230 235 240

Gly Gln Asp Ile Asn Tyr Gln Val Ser Arg Gln Ile Trp Gln Asp Met

245 250 255

Gln Tyr Asp Pro Arg Tyr Thr Pro Gly His Leu Gln Arg Ser Leu Val

260 265 270

Asp Ala Gly Leu Leu Gly Lys Lys Asn Gly Arg Ser Tyr Phe Ala Ala

275 280 285

Glu Glu Asn Ala Leu Pro Val Thr Ala Ala Thr Asp Ala Asp Val Glu

290 295 300

Thr Leu Arg Ile Tyr Gly Glu His Pro Phe Phe Thr Leu Leu Gln Gln

305 310 315 320

Arg Ala Ala Leu Gln Trp Pro Arg Leu Arg Val Glu Gln Arg Pro Ala

325 330 335

Leu Pro Gly Leu Gly Pro Ala Val Gln Ile Asn Glu Ala Phe Thr Val

340 345 350

Ser Val Thr Asp Gly Arg Thr Ala Ser Gln Leu Ala Glu Leu Thr Ala

355 360 365

Ala Asp Ala Phe Val Val Asp Val Ala Leu Asn Tyr Ala Asp Thr Ala

370 375 380

Tyr Leu Val Ala Ala His Asn Arg His Ala Ser Ala Ala Asn Lys Ala

385 390 395 400

Leu Phe Leu Arg Leu Leu His Thr Ala Ile Pro Gln Val Glu Phe Ile

405 410 415

Lys Asp Ser Pro Ala Leu Ile Val Ala Arg Val Leu Ser Ser Leu Ile

420 425 430

Asn Glu Ser Val Ile Met Val Glu Ser Gly Val Cys Ser Arg Glu Asp

435 440 445

Ile Asp Val Ala Ala Val Ala Gly Val Asn Tyr Ala Asp Gly Ile Phe

450 455 460

Gly Trp Leu Asp Asn Leu Gly Glu Lys Asn Val Arg Thr Thr Leu Ser

465 470 475 480

Asn Leu Ala Gln Leu Leu His Ala Ala Arg Tyr Ala Pro His Tyr Thr

485 490 495

Leu Leu His Ala Ala Gln Pro Ala Leu Thr Thr Thr Pro

500 505

<210> 45

<211> 509

<212> PRT

<213> Serratia marcescens SMB2099

<400> 45

Met Ala Glu Ser Asn Ala Ala Ile Gln Ser Ala Ala Ile Ile Gly Ala

1 5 10 15

Gly Thr Met Gly Arg Gly Ile Ala Tyr Leu Phe Ala Gln Lys Gly Ile

20 25 30

Arg Thr Val Leu Tyr Asn Arg Asn Gly Asn Thr Leu Asn Gln Ala Arg

35 40 45

Glu Tyr Ile Ala Gln Asp Leu Asn Lys Lys Val Glu Gln Gly Lys Ile

50 55 60

Ala Leu Gln Asp Lys Gly Ala Val Leu Ala Asn Leu Val Phe Thr Ser

65 70 75 80

Val Phe Glu Thr Ile Ala Asp Ser Asp Leu Val Ile Glu Thr Ile Ala

85 90 95

Glu Gln Glu Gln Thr Lys Leu Glu Val Leu Ala Ala Ile Ala Ala Ala

100 105 110

Val Lys Pro Asp Thr Leu Ile Ala Thr Asn Thr Ser Ser Leu Ser Leu

115 120 125

Asn Lys Leu Ala Thr Ala Val Thr His Ser Glu Arg Phe Ile Gly Leu

130 135 140

His Phe Phe Asn Pro Ala Pro Leu Met Lys Leu Ile Glu Ile Ile Pro

145 150 155 160

Ala Tyr Phe Thr Ala Arg Ala Thr Thr Glu Arg Cys Arg Gln Leu Val

165 170 175

Ala Ala Leu Gly Lys Arg Asp Val Val Cys Gln Ala Thr Pro Gly Phe

180 185 190

Ile Val Asn Arg Met Ala Arg Pro Tyr Tyr Leu Glu Gly Phe Arg Leu

195 200 205

Leu Glu Glu His Val Ala Arg Ala Pro Gln Ile Asp Arg Ala Leu Lys

210 215 220

Ala Gly Gly His Phe Arg Met Gly Pro Leu Glu Leu Thr Asp Phe Ile

225 230 235 240

Gly Gln Asp Ile Asn Tyr Gln Val Ser Arg Gln Ile Trp Gln Asp Met

245 250 255

Gln Tyr Asp Pro Arg Tyr Thr Pro Gly His Leu Gln Arg Ser Leu Val

260 265 270

Asp Ala Gly Leu Leu Gly Lys Lys Asn Gly Arg Ser Tyr Phe Ala Ala

275 280 285

Glu Glu Asn Ala Leu Pro Val Thr Ala Ala Thr Asp Ala Asp Val Glu

290 295 300

Thr Leu Arg Val Tyr Gly Glu His Pro Phe Phe Thr Leu Leu Gln Gln

305 310 315 320

Arg Ala Ala Leu Gln Trp Pro Arg Leu Arg Val Glu Gln Arg Pro Ala

325 330 335

Leu Pro Gly Leu Gly Pro Ala Val Gln Ile Asn Glu Ala Phe Thr Val

340 345 350

Ser Val Thr Asp Gly Arg Thr Ala Ser Gln Leu Ala Glu Leu Thr Ala

355 360 365

Ala Asp Ala Phe Val Val Asp Val Ala Leu Asn Tyr Ala Asp Thr Ala

370 375 380

Tyr Leu Val Ala Ala His Asn Arg His Ala Ser Glu Ala Asn Lys Ala

385 390 395 400

Leu Phe Leu Arg Leu Leu His Thr Ala Ile Pro Gln Val Glu Phe Ile

405 410 415

Lys Asp Ser Pro Ala Leu Ile Val Ala Arg Val Leu Ser Ser Leu Ile

420 425 430

Asn Glu Ser Val Ile Met Val Glu Ser Gly Val Cys Ser Arg Glu Asp

435 440 445

Ile Asp Val Ala Ala Val Ala Gly Val Asn Tyr Ala Asp Gly Ile Phe

450 455 460

Gly Trp Leu Asp Ser Leu Gly Glu Lys Asn Val Arg Thr Thr Leu Ser

465 470 475 480

Asn Leu Ala Gln Leu Leu His Ala Ala Arg Tyr Ala Pro His Tyr Thr

485 490 495

Leu Leu His Ala Ala Gln Pro Ala Leu Thr Thr Thr Pro

500 505

<210> 46

<211> 509

<212> PRT

<213> Serratia marcescens 2880STDY5682862

<400> 46

Met Ala Glu Ser Asn Ala Ala Ile Gln Ser Ala Ala Ile Ile Gly Ala

1 5 10 15

Gly Thr Met Gly Arg Gly Ile Ala Tyr Leu Phe Ala Gln Lys Gly Ile

20 25 30

Arg Thr Val Leu Tyr Asn Arg Asn Gly Asn Thr Leu Asn Gln Ala Arg

35 40 45

Glu Tyr Ile Val Gln Asp Leu Asn Lys Lys Val Glu Gln Gly Lys Ile

50 55 60

Ala Leu Gln Asp Lys Gly Ala Val Leu Ala Asn Leu Val Phe Thr Ser

65 70 75 80

Val Phe Glu Thr Ile Ala Asp Ser Glu Leu Val Ile Glu Thr Ile Ala

85 90 95

Glu Gln Glu Gln Thr Lys Leu Glu Val Leu Ala Ala Ile Ala Ala Ala

100 105 110

Val Lys Pro Asp Thr Leu Ile Ala Thr Asn Thr Ser Ser Leu Ser Leu

115 120 125

Asn Lys Leu Ala Thr Ala Val Thr His Ser Glu Arg Phe Ile Gly Leu

130 135 140

His Phe Phe Asn Pro Ala Pro Leu Met Lys Leu Ile Glu Ile Ile Pro

145 150 155 160

Ala Tyr Phe Thr Ala Arg Ala Thr Thr Glu Arg Cys Arg Gln Leu Val

165 170 175

Ala Ala Leu Gly Lys Arg Asp Val Val Cys Gln Ala Thr Pro Gly Phe

180 185 190

Ile Val Asn Arg Met Ala Arg Pro Tyr Tyr Leu Glu Gly Phe Arg Leu

195 200 205

Leu Glu Glu His Val Ala Arg Ala Pro Gln Ile Asp Arg Ala Leu Lys

210 215 220

Ala Gly Gly His Phe Arg Met Gly Pro Leu Glu Leu Thr Asp Phe Ile

225 230 235 240

Gly Gln Asp Ile Asn Tyr Gln Val Ser Arg Gln Ile Trp Gln Asp Met

245 250 255

Gln Tyr Asp Pro Arg Tyr Thr Pro Gly His Leu Gln Arg Ser Leu Val

260 265 270

Asp Ala Gly Leu Leu Gly Lys Lys Asn Gly Arg Ser Tyr Phe Ala Ala

275 280 285

Glu Glu Asn Ala Leu Pro Val Thr Ala Ala Thr Asp Ala Asp Val Glu

290 295 300

Thr Leu Arg Val Tyr Gly Glu His Pro Phe Phe Thr Leu Leu Gln Gln

305 310 315 320

Arg Ala Ala Leu Gln Trp Pro Arg Leu Arg Val Glu Gln Arg Pro Ala

325 330 335

Leu Pro Gly Leu Gly Pro Ala Val Gln Ile Asn Glu Ala Phe Thr Val

340 345 350

Ser Val Thr Asp Gly Arg Thr Ala Ser Gln Leu Ala Glu Leu Thr Ala

355 360 365

Ala Asp Ala Phe Val Val Asp Val Ala Leu Asn Tyr Ala Asp Thr Ala

370 375 380

Tyr Leu Val Ala Ala His Asn Arg His Ala Ser Ala Ala Asn Lys Ala

385 390 395 400

Leu Phe Leu Arg Leu Leu His Thr Ala Ile Pro Gln Val Glu Phe Ile

405 410 415

Lys Asp Ser Pro Ala Leu Ile Val Ala Arg Val Leu Ser Ser Leu Ile

420 425 430

Asn Glu Ser Val Ile Met Val Glu Ser Gly Val Cys Ser Arg Glu Asp

435 440 445

Ile Asp Val Ala Ala Val Ala Gly Val Asn Tyr Ala Asp Gly Ile Phe

450 455 460

Gly Trp Leu Asp Ser Leu Gly Glu Lys Asn Val Arg Thr Thr Leu Ser

465 470 475 480

Asn Met Ala Gln Leu Leu His Ala Ala Arg Tyr Ala Pro His Tyr Thr

485 490 495

Leu Leu His Ala Ala Gln Pro Ala Leu Met Thr Thr Pro

500 505

<210> 47

<211> 509

<212> PRT

<213> Serratia marcescens SE4145

<400> 47

Met Ala Glu Ser Asn Ala Glu Ile Gln Ser Ala Ala Ile Ile Gly Ala

1 5 10 15

Gly Thr Met Gly Arg Gly Ile Ala Tyr Leu Phe Ala Gln Lys Gly Ile

20 25 30

Arg Thr Val Leu Tyr Asn Arg Asn Gly Asn Thr Leu Asn Gln Ala Arg

35 40 45

Glu Tyr Ile Val Gln Asp Leu Asn Lys Lys Val Glu Gln Gly Lys Ile

50 55 60

Ala Leu Gln Asp Lys Gly Ala Val Leu Ala Asn Leu Val Phe Thr Ser

65 70 75 80

Val Phe Glu Thr Ile Ala Asp Ser Glu Leu Val Ile Glu Thr Ile Ala

85 90 95

Glu Gln Glu Gln Thr Lys Leu Glu Val Leu Ala Ala Ile Ala Ala Ala

100 105 110

Val Lys Pro Asp Thr Leu Ile Ala Thr Asn Thr Ser Ser Leu Ser Leu

115 120 125

Asn Lys Leu Ala Thr Ala Val Thr His Ser Glu Arg Phe Ile Gly Leu

130 135 140

His Phe Phe Asn Pro Ala Pro Leu Met Lys Leu Ile Glu Ile Ile Pro

145 150 155 160

Ala Tyr Phe Thr Ala Arg Ala Thr Thr Glu Arg Cys Arg Gln Leu Val

165 170 175

Ala Ala Leu Gly Lys Arg Asp Val Val Cys Gln Ala Thr Pro Gly Phe

180 185 190

Ile Val Asn Arg Met Ala Arg Pro Tyr Tyr Leu Glu Gly Phe Arg Leu

195 200 205

Leu Glu Glu His Val Ala Arg Ala Pro Gln Ile Asp Arg Ala Leu Lys

210 215 220

Ala Gly Gly His Phe Arg Met Gly Pro Leu Glu Leu Thr Asp Phe Ile

225 230 235 240

Gly Gln Asp Ile Asn Tyr Gln Val Ser Arg Gln Ile Trp Gln Asp Met

245 250 255

Gln Tyr Asp Pro Arg Tyr Thr Pro Gly His Leu Gln Arg Ser Leu Val

260 265 270

Asp Ala Gly Leu Leu Gly Lys Lys Asn Gly Arg Ser Tyr Phe Ala Ala

275 280 285

Glu Glu Asn Ala Leu Pro Val Thr Ala Ala Thr Asp Ala Asp Val Glu

290 295 300

Thr Leu Arg Ile Tyr Gly Glu His Pro Phe Phe Thr Leu Leu Gln Gln

305 310 315 320

Arg Ala Ala Leu Gln Trp Pro Arg Leu Arg Val Glu Gln Arg Pro Ala

325 330 335

Leu Pro Gly Leu Gly Pro Ala Val Gln Ile Asn Glu Ala Phe Thr Val

340 345 350

Ser Val Thr Asp Gly Arg Thr Ala Ser Gln Leu Ala Glu Leu Thr Ala

355 360 365

Ala Asp Ala Phe Val Val Asp Val Ala Leu Asn Tyr Ala Asp Thr Ala

370 375 380

Tyr Leu Val Ala Ala His Asn Arg His Ala Ser Ala Ala Asn Lys Ala

385 390 395 400

Leu Phe Leu Arg Leu Leu His Thr Ala Ile Pro Gln Val Glu Phe Ile

405 410 415

Lys Asp Ser Pro Ala Leu Ile Val Ala Arg Val Leu Ser Ser Leu Ile

420 425 430

Asn Glu Ser Val Ile Met Val Glu Ser Gly Val Cys Ser Arg Glu Asp

435 440 445

Ile Asp Val Ala Ala Val Ala Gly Val Asn Tyr Ala Asp Gly Ile Phe

450 455 460

Gly Trp Leu Asp Asn Leu Gly Glu Lys Asn Val Arg Thr Thr Leu Ser

465 470 475 480

Asn Leu Ala Gln Leu Leu His Ala Ala Arg Tyr Ala Pro His Tyr Thr

485 490 495

Leu Leu His Ala Ala Gln Pro Ala Leu Thr Thr Thr Pro

500 505

<210> 48

<211> 509

<212> PRT

<213> Serratia marcescens 2880STDY5682876

<400> 48

Met Ala Glu Ser Asn Ala Ala Ile Gln Ser Ala Ala Ile Ile Gly Ala

1 5 10 15

Gly Thr Met Gly Arg Gly Ile Ala Tyr Leu Phe Ala Gln Lys Gly Ile

20 25 30

Arg Thr Val Leu Tyr Asn Arg Asn Gly Asn Thr Leu Asn Gln Ala Arg

35 40 45

Glu Tyr Ile Ala Gln Asp Leu Asn Lys Lys Val Glu Gln Gly Lys Ile

50 55 60

Ala Leu Gln Gly Lys Gly Ala Val Leu Ala Asn Leu Val Phe Thr Ser

65 70 75 80

Val Phe Glu Thr Ile Ala Asp Ser Asp Leu Val Ile Glu Thr Ile Ala

85 90 95

Glu Gln Glu Gln Thr Lys Leu Glu Val Leu Ala Ala Ile Ala Ala Ala

100 105 110

Val Lys Pro Asp Thr Leu Ile Ala Thr Asn Thr Ser Ser Leu Ser Leu

115 120 125

Asn Lys Leu Ala Thr Ala Val Thr His Ser Glu Arg Phe Ile Gly Leu

130 135 140

His Phe Phe Asn Pro Ala Pro Leu Met Lys Leu Ile Glu Ile Ile Pro

145 150 155 160

Ala Tyr Phe Thr Ala Arg Ala Thr Thr Glu Arg Cys Arg Gln Leu Val

165 170 175

Ala Ala Leu Gly Lys Arg Asp Val Val Cys Gln Ala Thr Pro Gly Phe

180 185 190

Ile Val Asn Arg Met Ala Arg Pro Tyr Tyr Leu Glu Gly Phe Arg Leu

195 200 205

Leu Glu Glu His Val Ala Arg Ala Pro Gln Ile Asp Arg Ala Leu Lys

210 215 220

Ala Gly Gly His Phe Arg Met Gly Pro Leu Glu Leu Thr Asp Phe Ile

225 230 235 240

Gly Gln Asp Ile Asn Tyr Gln Val Ser Arg Gln Ile Trp Gln Asp Met

245 250 255

Gln Tyr Asp Pro Arg Tyr Thr Pro Gly His Leu Gln Arg Ser Leu Val

260 265 270

Asp Ala Gly Leu Leu Gly Lys Lys Asn Gly Arg Ser Tyr Phe Ala Ala

275 280 285

Glu Glu Asn Ala Leu Pro Val Thr Ala Ala Thr Asp Ala Asp Val Glu

290 295 300

Thr Leu Arg Val Tyr Gly Glu His Pro Phe Phe Thr Leu Leu Gln Gln

305 310 315 320

Arg Ala Ala Leu Gln Trp Pro Arg Leu Arg Val Glu Gln Arg Pro Ala

325 330 335

Leu Pro Gly Leu Gly Pro Ala Val Gln Ile Asn Glu Ala Phe Thr Val

340 345 350

Ser Val Thr Asp Gly Arg Thr Ala Ser Gln Leu Ala Glu Leu Thr Ala

355 360 365

Ala Asp Ala Phe Val Val Asp Val Ala Leu Asn Tyr Ala Asp Thr Ala

370 375 380

Tyr Leu Val Ala Ala His Asn Arg His Ala Ser Ala Ala Asn Lys Ala

385 390 395 400

Leu Phe Leu Arg Leu Leu His Thr Ala Ile Pro Gln Val Glu Phe Ile

405 410 415

Lys Asp Ser Pro Ala Leu Ile Val Ala Arg Val Leu Ser Ser Leu Ile

420 425 430

Asn Glu Ser Val Ile Met Val Glu Ser Gly Val Cys Ser Arg Glu Asp

435 440 445

Ile Asp Val Ala Ala Val Ala Gly Val Asn Tyr Ala Asp Gly Ile Phe

450 455 460

Gly Trp Leu Asp Ser Leu Gly Glu Lys Asn Val Arg Thr Thr Leu Ser

465 470 475 480

Asn Leu Ala Gln Leu Leu His Ala Ala Arg Tyr Ala Pro His Tyr Thr

485 490 495

Leu Leu His Ala Ala Gln Pro Ala Leu Thr Thr Thr Pro

500 505

<210> 49

<211> 509

<212> PRT

<213> Serratia marcescens 709

<400> 49

Met Ala Glu Ser Asn Ala Ala Ile Gln Ser Ala Ala Ile Ile Gly Ala

1 5 10 15

Gly Thr Met Gly Arg Gly Ile Ala Tyr Leu Phe Ala Gln Lys Gly Ile

20 25 30

Arg Thr Val Leu Tyr Asn Arg Asn Gly Asn Thr Leu Asn Gln Ala Arg

35 40 45

Glu Tyr Ile Ala Gln Asp Leu Asn Lys Lys Val Glu Gln Gly Lys Ile

50 55 60

Ala Leu Gln Asp Lys Gly Ala Val Leu Ala Asn Leu Val Phe Thr Ser

65 70 75 80

Val Phe Glu Thr Ile Ala Asp Ser Asp Leu Val Ile Glu Thr Ile Ala

85 90 95

Glu Gln Glu Gln Thr Lys Leu Glu Val Leu Ala Ala Ile Ala Ala Ala

100 105 110

Val Lys Pro Asp Thr Leu Ile Ala Thr Asn Thr Ser Ser Leu Ser Leu

115 120 125

Asn Lys Leu Ala Thr Ala Val Thr His Ser Glu Arg Phe Ile Gly Leu

130 135 140

His Phe Phe Asn Pro Ala Pro Leu Met Lys Leu Ile Glu Ile Ile Pro

145 150 155 160

Ala Tyr Phe Thr Ala Arg Ala Thr Thr Glu Arg Cys Arg Gln Leu Val

165 170 175

Ala Ala Leu Gly Lys Arg Asp Val Val Cys Gln Ala Thr Pro Gly Phe

180 185 190

Ile Val Asn Arg Met Ala Arg Pro Tyr Tyr Leu Glu Gly Phe Arg Leu

195 200 205

Leu Glu Glu His Val Ala Arg Ala Pro Gln Ile Asp Arg Ala Leu Lys

210 215 220

Ala Gly Gly His Phe Arg Met Gly Pro Leu Glu Leu Thr Asp Phe Ile

225 230 235 240

Gly Gln Asp Ile Asn Tyr Gln Val Ser Arg Gln Ile Trp Gln Asp Met

245 250 255

Gln Tyr Asp Pro Arg Tyr Thr Pro Gly His Leu Gln Arg Ser Leu Val

260 265 270

Asp Ala Gly Leu Leu Gly Lys Lys Asn Gly Arg Ser Tyr Phe Ala Ala

275 280 285

Glu Glu Asn Ala Leu Pro Val Thr Ala Ala Thr Asp Ala Asp Val Glu

290 295 300

Thr Leu Arg Val Tyr Asp Glu His Pro Phe Phe Thr Leu Leu Gln Gln

305 310 315 320

Arg Ala Ala Leu Gln Trp Pro Arg Leu Arg Val Glu Gln Arg Pro Ala

325 330 335

Leu Pro Gly Leu Gly Pro Ala Val Gln Ile Asn Glu Ala Phe Thr Val

340 345 350

Ser Val Thr Asp Gly Arg Thr Ala Ser Gln Leu Ala Glu Leu Thr Ala

355 360 365

Ala Asp Ala Phe Val Val Asp Val Ala Leu Asn Tyr Ala Asp Thr Ala

370 375 380

Tyr Leu Val Ala Ala His Asn Arg His Ala Ser Ala Ala Asn Lys Ala

385 390 395 400

Leu Phe Leu Arg Leu Leu His Thr Ala Ile Pro Gln Val Glu Phe Ile

405 410 415

Lys Asp Ser Pro Ala Leu Ile Val Ala Arg Val Leu Ser Ser Leu Ile

420 425 430

Asn Glu Ser Val Ile Met Val Glu Ser Gly Val Cys Ser Arg Glu Asp

435 440 445

Ile Asp Val Ala Ala Val Ala Gly Val Asn Tyr Ala Asp Gly Ile Phe

450 455 460

Gly Trp Leu Asp Ser Leu Gly Glu Lys Asn Val Arg Thr Thr Leu Ser

465 470 475 480

Asn Leu Ala Gln Leu Leu His Ala Ala Arg Tyr Ala Pro His Tyr Thr

485 490 495

Leu Leu His Ala Ala Gln Pro Ala Leu Thr Thr Thr Pro

500 505

<210> 50

<211> 509

<212> PRT

<213> Serratia marcescens MGH136

<400> 50

Met Ala Glu Ser Asn Ala Ala Ile Gln Ser Ala Ala Ile Ile Gly Ala

1 5 10 15

Gly Thr Met Gly Arg Gly Ile Ala Tyr Leu Phe Ala Gln Lys Gly Ile

20 25 30

Arg Thr Val Leu Tyr Asn Arg Asn Gly Asn Thr Leu Asn Gln Ala Arg

35 40 45

Glu Tyr Ile Ala Gln Asp Leu Asn Lys Lys Val Glu Gln Asp Lys Ile

50 55 60

Ala Leu Gln Asp Lys Gly Ala Val Leu Ala Asn Leu Val Phe Thr Ser

65 70 75 80

Val Phe Glu Thr Ile Ala Asp Ser Asp Leu Val Ile Glu Thr Ile Ala

85 90 95

Glu Gln Glu Gln Thr Lys Leu Glu Val Leu Ala Ala Ile Ala Ala Ala

100 105 110

Val Lys Pro Asp Thr Leu Ile Ala Thr Asn Thr Ser Ser Leu Ser Leu

115 120 125

Asn Lys Leu Ala Thr Ala Val Thr His Ser Glu Arg Phe Ile Gly Leu

130 135 140

His Phe Phe Asn Pro Ala Pro Leu Met Lys Leu Ile Glu Ile Ile Pro

145 150 155 160

Ala Tyr Phe Thr Ala Arg Val Thr Thr Glu Arg Cys Arg Gln Leu Val

165 170 175

Ala Ala Leu Gly Lys Arg Asp Val Val Cys Gln Ala Thr Pro Gly Phe

180 185 190

Ile Val Asn Arg Met Ala Arg Pro Tyr Tyr Leu Glu Gly Phe Arg Leu

195 200 205

Leu Glu Glu His Val Ala Arg Ala Pro Gln Ile Asp Arg Ala Leu Lys

210 215 220

Ala Gly Gly His Phe Arg Met Gly Pro Leu Glu Leu Thr Asp Phe Ile

225 230 235 240

Gly Gln Asp Ile Asn Tyr Gln Val Ser Arg Gln Ile Trp Gln Asp Met

245 250 255

Gln Tyr Asp Pro Arg Tyr Thr Pro Gly His Leu Gln Arg Ser Leu Val

260 265 270

Asp Ala Gly Leu Leu Gly Lys Lys Asn Gly Arg Ser Tyr Phe Ala Ala

275 280 285

Glu Glu Asn Ala Leu Pro Val Thr Ala Ala Thr Asp Ala Asp Val Glu

290 295 300

Thr Leu Arg Val Tyr Gly Glu His Pro Phe Phe Thr Leu Leu Gln Gln

305 310 315 320

Arg Ala Ala Leu Gln Trp Pro Arg Leu Arg Val Glu Gln Arg Pro Ala

325 330 335

Leu Pro Gly Leu Gly Pro Ala Val Gln Ile Asn Glu Ala Phe Thr Val

340 345 350

Ser Val Thr Asp Gly Arg Thr Ala Ser Gln Leu Ala Glu Leu Thr Ala

355 360 365

Ala Asp Ala Phe Val Val Asp Val Ala Leu Asn Tyr Ala Asp Thr Ala

370 375 380

Tyr Leu Val Ala Ala His Asn Arg His Ala Ser Ala Ala Asn Lys Ala

385 390 395 400

Leu Phe Leu Arg Leu Leu His Thr Ala Ile Pro Gln Val Glu Phe Ile

405 410 415

Lys Asp Ser Pro Ala Leu Ile Val Ala Arg Val Leu Ser Ser Leu Ile

420 425 430

Asn Glu Ser Val Ile Met Val Glu Ser Gly Val Cys Ser Arg Glu Asp

435 440 445

Ile Asp Val Ala Ala Val Ala Gly Val Asn Tyr Ala Asp Gly Ile Phe

450 455 460

Gly Trp Leu Asp Ser Leu Gly Glu Lys Asn Val Arg Thr Thr Leu Ser

465 470 475 480

Asn Leu Ala Gln Leu Leu His Ala Ala Arg Tyr Ala Pro His Tyr Thr

485 490 495

Leu Leu His Ala Ala Gln Pro Ala Leu Thr Thr Thr Pro

500 505

<210> 51

<211> 509

<212> PRT

<213> Serratia marcescens 2880STDY5682884

<400> 51

Met Ala Glu Ser Asn Ala Ala Ile Gln Ser Ala Ala Ile Ile Gly Ala

1 5 10 15

Gly Thr Met Gly Arg Gly Ile Ala Tyr Leu Phe Ala Gln Lys Gly Ile

20 25 30

Arg Thr Val Leu Tyr Asn Arg Asn Gly Asn Thr Leu Asn Gln Ala Arg

35 40 45

Glu Tyr Ile Ala Gln Asp Leu Asn Lys Lys Val Glu Gln Gly Lys Ile

50 55 60

Ala Leu Gln Asp Lys Gly Ala Val Leu Ala Asn Leu Val Phe Thr Ser

65 70 75 80

Val Phe Glu Thr Ile Ala Asp Ser Glu Leu Val Ile Glu Thr Ile Ala

85 90 95

Glu Gln Glu Gln Thr Lys Leu Glu Val Leu Ala Ala Ile Ala Ala Ala

100 105 110

Val Lys Pro Asp Thr Leu Ile Ala Thr Asn Thr Ser Ser Leu Ser Leu

115 120 125

Asn Lys Leu Ala Thr Ala Val Thr His Ser Glu Arg Phe Ile Gly Leu

130 135 140

His Phe Phe Asn Pro Ala Pro Leu Met Lys Leu Ile Glu Ile Ile Pro

145 150 155 160

Ala Tyr Phe Thr Ala Arg Ala Thr Thr Glu Arg Cys Arg Gln Leu Val

165 170 175

Ala Ala Leu Gly Lys Arg Asp Val Val Cys Gln Ala Thr Pro Gly Phe

180 185 190

Ile Val Asn Arg Met Ala Arg Pro Tyr Tyr Leu Glu Gly Phe Arg Leu

195 200 205

Leu Glu Glu His Val Ala Arg Ala Pro Gln Ile Asp Arg Ala Leu Lys

210 215 220

Ala Gly Gly His Phe Arg Met Gly Pro Leu Glu Leu Thr Asp Phe Ile

225 230 235 240

Gly Gln Asp Ile Asn Tyr Gln Val Ser Arg Gln Ile Trp Gln Asp Met

245 250 255

Gln Tyr Asp Pro Arg Tyr Thr Pro Gly His Leu Gln Arg Ser Leu Val

260 265 270

Asp Ala Gly Leu Leu Gly Lys Lys Asn Gly Arg Ser Tyr Phe Ala Ala

275 280 285

Glu Glu Asn Ala Leu Pro Val Thr Ala Ala Thr Asp Ala Asp Val Glu

290 295 300

Thr Leu Arg Ile Tyr Gly Glu His Pro Phe Phe Thr Leu Leu Gln Gln

305 310 315 320

Arg Ala Thr Leu Gln Trp Pro Arg Leu Arg Val Glu Gln Arg Pro Ala

325 330 335

Leu Pro Gly Leu Gly Pro Ala Val Gln Ile Asn Glu Ala Phe Thr Val

340 345 350

Ser Val Thr Asp Gly Arg Thr Ala Ser Gln Leu Ala Glu Leu Met Ala

355 360 365

Ala Asp Ala Phe Val Val Asp Val Ala Leu Asn Tyr Ala Asp Thr Ala

370 375 380

Tyr Leu Val Ala Ala His Asn Arg His Ala Ser Ala Ala Asn Lys Ala

385 390 395 400

Leu Phe Leu Arg Leu Leu His Thr Ala Ile Pro Gln Val Glu Phe Ile

405 410 415

Lys Asp Ser Pro Ala Leu Ile Val Ala Arg Val Leu Ser Ser Leu Ile

420 425 430

Asn Glu Ser Val Ile Met Val Glu Ser Gly Val Cys Ser Arg Glu Asp

435 440 445

Ile Asp Val Ala Ala Val Ala Gly Val Asn Tyr Ala Asp Gly Ile Phe

450 455 460

Gly Trp Leu Asp Ser Leu Gly Glu Lys Asn Val Arg Met Thr Leu Ser

465 470 475 480

Asn Leu Ala Gln Leu Leu His Ala Ala Arg Tyr Ala Pro His Tyr Thr

485 490 495

Leu Leu His Ala Ala Gln Pro Ala Leu Thr Thr Thr Pro

500 505

<210> 52

<211> 509

<212> PRT

<213> Serratia marcescens D-3

<400> 52

Met Ala Glu Ser Asn Ala Ala Ile Gln Ser Ala Ala Ile Ile Gly Ala

1 5 10 15

Gly Thr Met Gly Arg Gly Ile Ala Tyr Leu Phe Ala Gln Lys Gly Ile

20 25 30

Arg Thr Val Leu Tyr Asn Arg Asn Gly Asn Thr Leu Asn Gln Ala Arg

35 40 45

Glu Tyr Ile Ala Gln Asp Leu Asn Lys Lys Val Glu Gln Gly Lys Ile

50 55 60

Ala Leu Gln Asp Lys Gly Ala Val Leu Ala Asn Leu Val Phe Thr Ser

65 70 75 80

Val Phe Glu Thr Ile Ala Asp Ser Asp Leu Val Ile Glu Thr Ile Ala

85 90 95

Glu Gln Glu Gln Thr Lys Leu Glu Val Leu Ala Ala Ile Ala Ala Ala

100 105 110

Val Lys Pro Asp Thr Leu Ile Ala Thr Asn Thr Ser Ser Leu Ser Leu

115 120 125

Asn Lys Leu Ala Thr Ala Val Thr His Ser Glu Arg Phe Ile Gly Leu

130 135 140

His Phe Phe Asn Pro Ala Pro Leu Met Lys Leu Ile Glu Ile Ile Pro

145 150 155 160

Ala Tyr Phe Thr Ala Arg Ala Thr Thr Glu Arg Cys Arg Gln Leu Val

165 170 175

Ala Ala Leu Gly Lys Arg Asp Val Val Cys Gln Ala Thr Pro Gly Phe

180 185 190

Ile Val Asn Arg Met Ala Arg Pro Tyr Tyr Leu Glu Gly Phe Arg Leu

195 200 205

Leu Glu Glu His Val Ala Arg Ala Pro Gln Ile Asp Arg Ala Leu Lys

210 215 220

Ala Gly Gly His Phe Arg Met Gly Pro Leu Glu Leu Thr Asp Phe Ile

225 230 235 240

Gly Gln Asp Ile Asn Tyr Gln Val Ser Arg Gln Ile Trp Gln Asp Met

245 250 255

Gln Tyr Asp Pro Arg Tyr Thr Pro Gly His Leu Gln Arg Ser Leu Val

260 265 270

Asp Ala Gly Leu Leu Gly Lys Lys Asn Gly Arg Ser Tyr Phe Ala Ala

275 280 285

Glu Glu Asn Ala Leu Pro Val Thr Ala Ala Ile Asp Ala Asp Val Glu

290 295 300

Thr Leu Arg Val Tyr Gly Glu His Pro Phe Phe Thr Leu Leu Gln Gln

305 310 315 320

Arg Ala Ala Leu Gln Trp Pro Arg Leu Arg Val Glu Gln Arg Pro Ala

325 330 335

Leu Pro Gly Leu Gly Pro Ala Val Gln Ile Asn Glu Ala Phe Thr Val

340 345 350

Ser Val Thr Asp Gly Arg Thr Ala Ser Gln Leu Ala Glu Leu Thr Ala

355 360 365

Ala Asp Ala Phe Val Val Asp Val Ala Leu Asn Tyr Ala Asp Thr Ala

370 375 380

Tyr Leu Val Ala Ala His Asn Arg His Ala Ser Ala Ala Asn Lys Ala

385 390 395 400

Leu Phe Leu Arg Leu Leu His Thr Ala Ile Pro Gln Val Glu Phe Ile

405 410 415

Lys Asp Ser Pro Ala Leu Ile Val Ala Arg Val Leu Ser Ser Leu Ile

420 425 430

Asn Glu Ser Val Ile Met Val Glu Ser Gly Val Cys Ser Arg Glu Asp

435 440 445

Ile Asp Val Ala Ala Val Ala Gly Val Asn Tyr Ala Asp Gly Ile Phe

450 455 460

Gly Leu Leu Asp Ser Leu Gly Glu Lys Asn Val Arg Thr Thr Leu Ser

465 470 475 480

Asn Leu Ala Gln Leu Leu His Ala Ala Arg Tyr Ala Pro His Tyr Thr

485 490 495

Leu Leu His Ala Ala Gln Pro Ala Leu Thr Thr Thr Pro

500 505

<210> 53

<211> 509

<212> PRT

<213> Serratia marcescens 2880STDY5682957

<400> 53

Met Ala Glu Ser Asn Ala Ala Ile Gln Ser Ala Ala Ile Ile Gly Ala

1 5 10 15

Gly Thr Met Gly Arg Gly Ile Ala Tyr Leu Phe Ala Gln Lys Gly Ile

20 25 30

Arg Thr Val Leu Tyr Asn Arg Asn Gly Asn Thr Leu Asn Gln Ala Arg

35 40 45

Glu Tyr Ile Glu Leu Asp Leu Asn Lys Lys Val Glu Gln Gly Lys Ile

50 55 60

Ala Leu Gln Asp Lys Gly Ala Val Leu Ala Asn Leu Val Phe Thr Ser

65 70 75 80

Ala Phe Glu Thr Ile Ala Asp Ser Glu Leu Val Ile Glu Thr Ile Ala

85 90 95

Glu Gln Glu Gln Thr Lys Leu Glu Val Leu Ala Ala Ile Ala Ala Ala

100 105 110

Val Lys Pro Asp Thr Leu Ile Ala Thr Asn Thr Ser Ser Leu Ser Leu

115 120 125

Asn Lys Leu Ala Thr Ala Val Thr His Ser Glu Arg Phe Ile Gly Leu

130 135 140

His Phe Phe Asn Pro Ala Pro Leu Met Lys Leu Ile Glu Ile Ile Pro

145 150 155 160

Ala Tyr Phe Thr Ala Arg Ala Thr Thr Glu Arg Cys Arg Gln Leu Val

165 170 175

Ala Ala Leu Gly Lys Arg Asp Val Val Cys Gln Ala Thr Pro Gly Phe

180 185 190

Ile Val Asn Arg Met Ala Arg Pro Tyr Tyr Leu Glu Gly Phe Arg Leu

195 200 205

Leu Glu Glu His Val Ala Arg Ala Pro Gln Ile Asp Arg Ala Leu Lys

210 215 220

Ala Gly Gly His Phe Arg Met Gly Pro Leu Glu Leu Thr Asp Phe Ile

225 230 235 240

Gly Gln Asp Ile Asn Tyr Gln Val Ser Arg Gln Ile Trp Gln Asp Met

245 250 255

Gln Tyr Asp Pro Arg Tyr Thr Pro Gly His Leu Gln Arg Ser Leu Val

260 265 270

Asp Ala Gly Leu Leu Gly Lys Lys Asn Gly Arg Ser Tyr Phe Ala Ala

275 280 285

Glu Glu Asn Ala Leu Pro Val Thr Ala Ala Thr Asp Ala Asp Val Glu

290 295 300

Thr Leu Arg Val Tyr Gly Glu His Pro Phe Phe Thr Leu Leu Gln Gln

305 310 315 320

Arg Ala Ala Leu Gln Trp Pro Arg Leu Arg Val Glu Gln Arg Pro Ala

325 330 335

Leu Pro Gly Leu Gly Pro Ala Val Gln Ile Asn Glu Ala Phe Thr Val

340 345 350

Ser Val Thr Asp Gly Arg Thr Ala Ser Gln Leu Ala Glu Leu Thr Ala

355 360 365

Ala Asp Ala Phe Val Val Asp Val Ala Leu Asn Tyr Ala Asp Thr Ala

370 375 380

Tyr Leu Val Ala Ala His Asn Arg His Ala Ser Ala Ala Asn Lys Ala

385 390 395 400

Leu Phe Leu Arg Leu Leu His Thr Ala Ile Pro Gln Val Glu Phe Ile

405 410 415

Lys Asp Ser Pro Ala Leu Ile Val Ala Arg Val Leu Ser Ser Leu Ile

420 425 430

Asn Glu Ser Val Ile Met Val Glu Ser Gly Val Cys Ser Arg Glu Asp

435 440 445

Ile Asp Val Ala Ala Val Ala Gly Val Asn Tyr Ala Asp Gly Ile Phe

450 455 460

Gly Trp Leu Asp Ser Leu Gly Glu Lys Asn Val Arg Thr Thr Leu Ser

465 470 475 480

Asn Leu Ala Gln Leu Leu His Ala Ala Arg Tyr Ala Pro His Tyr Thr

485 490 495

Leu Leu His Ala Ala Gln Pro Ala Leu Thr Thr Thr Pro

500 505

<210> 54

<211> 509

<212> PRT

<213> Serratia marcescens YDC563

<400> 54

Met Ala Glu Ser Asn Ala Ala Ile Gln Ser Ala Ala Ile Ile Gly Ala

1 5 10 15

Gly Thr Met Gly Arg Gly Ile Ala Tyr Leu Phe Ala Gln Lys Gly Ile

20 25 30

Arg Thr Val Leu Tyr Asn Arg Asn Gly Asn Thr Leu Asn Gln Ala Arg

35 40 45

Glu Tyr Ile Glu Leu Asp Leu Asn Lys Lys Val Glu Gln Gly Lys Ile

50 55 60

Ala Leu Gln Asp Lys Gly Ala Val Leu Ala Asn Leu Val Phe Thr Ser

65 70 75 80

Ala Phe Glu Thr Ile Ala Asp Ser Glu Leu Val Ile Glu Thr Ile Ala

85 90 95

Glu Gln Glu Gln Thr Lys Leu Glu Val Leu Ala Ala Ile Ala Ala Ala

100 105 110

Val Lys Pro Asp Thr Leu Ile Ala Thr Asn Thr Ser Ser Leu Ser Leu

115 120 125

Asn Lys Leu Ala Thr Ala Val Thr His Ser Glu Arg Phe Ile Gly Leu

130 135 140

His Phe Phe Asn Pro Ala Pro Leu Met Lys Leu Ile Glu Ile Ile Pro

145 150 155 160

Ala Tyr Phe Thr Ala Arg Ala Thr Thr Glu Arg Cys Arg Gln Leu Val

165 170 175

Ala Ala Leu Gly Lys Arg Asp Val Val Cys Gln Ala Thr Pro Gly Phe

180 185 190

Ile Val Asn Arg Met Ala Arg Pro Tyr Tyr Leu Glu Gly Phe Arg Leu

195 200 205

Leu Glu Glu His Val Ala Arg Ala Pro Gln Ile Asp Arg Ala Leu Lys

210 215 220

Ala Gly Gly His Phe Arg Met Gly Pro Leu Glu Leu Thr Asp Phe Ile

225 230 235 240

Gly Gln Asp Ile Asn Tyr Gln Val Ser Arg Gln Ile Trp Gln Asp Met

245 250 255

Gln Tyr Asp Pro Arg Tyr Thr Pro Gly His Leu Gln Arg Ser Leu Val

260 265 270

Asp Ala Gly Leu Leu Gly Lys Lys Asn Gly Arg Ser Tyr Phe Ala Ala

275 280 285

Glu Glu Asn Ala Leu Pro Val Thr Ala Ala Thr Asp Ala Asp Val Glu

290 295 300

Thr Leu Arg Val Tyr Gly Glu His Pro Phe Phe Thr Leu Leu Gln Gln

305 310 315 320

Arg Ala Ala Leu Gln Trp Pro Arg Leu Arg Val Glu Gln Arg Pro Ala

325 330 335

Leu Pro Gly Leu Gly Pro Ala Val Gln Ile Asn Glu Ala Phe Thr Val

340 345 350

Ser Val Thr Asp Gly Arg Thr Ala Ser Gln Leu Ala Glu Gln Thr Ala

355 360 365

Ala Asp Ala Phe Val Val Asp Val Ala Leu Asn Tyr Ala Asp Thr Ala

370 375 380

Tyr Leu Val Ala Ala His Asn Arg His Ala Ser Ala Ala Asn Lys Ala

385 390 395 400

Leu Phe Leu Arg Leu Leu His Thr Ala Ile Pro Gln Val Glu Phe Ile

405 410 415

Lys Asp Ser Pro Ala Leu Ile Val Ala Arg Val Leu Ser Ser Leu Ile

420 425 430

Asn Glu Ser Val Ile Met Val Glu Ser Gly Val Cys Ser Arg Glu Asp

435 440 445

Ile Asp Val Ala Ala Val Ala Gly Val Asn Tyr Ala Asp Gly Ile Phe

450 455 460

Gly Trp Leu Asp Ser Leu Gly Glu Lys Asn Val Arg Thr Thr Leu Ser

465 470 475 480

Asn Met Ala Gln Leu Leu His Ala Ala Arg Tyr Ala Pro His Tyr Thr

485 490 495

Leu Leu His Ala Ala Gln Pro Ala Leu Met Thr Thr Pro

500 505

<210> 55

<211> 509

<212> PRT

<213> Serratia marcescens 2880STDY5683035

<400> 55

Met Ala Glu Ser Asn Ala Ala Ile Gln Ser Ala Ala Ile Ile Gly Ala

1 5 10 15

Gly Thr Met Gly Arg Gly Ile Ala Tyr Leu Phe Ala Gln Lys Gly Ile

20 25 30

Arg Thr Val Leu Tyr Asn Arg Asn Gly Asn Thr Leu Asn Gln Ala Arg

35 40 45

Glu Tyr Ile Ala Gln Asp Leu Asn Lys Lys Val Glu Gln Gly Lys Ile

50 55 60

Ala Leu Gln Asp Lys Gly Ala Val Leu Ala Asn Leu Val Phe Thr Ser

65 70 75 80

Val Phe Glu Thr Ile Ala Asp Ser Asp Leu Val Ile Glu Thr Ile Ala

85 90 95

Glu Gln Glu Gln Thr Lys Leu Glu Val Leu Ala Ala Ile Ala Ala Ala

100 105 110

Val Lys Pro Asp Thr Leu Ile Ala Thr Asn Thr Ser Ser Leu Ser Leu

115 120 125

Asn Lys Leu Ala Thr Ala Val Thr His Ser Glu Arg Phe Ile Gly Leu

130 135 140

His Phe Phe Asn Pro Ala Pro Leu Met Lys Leu Ile Glu Ile Ile Pro

145 150 155 160

Ala Tyr Phe Thr Ala Arg Ala Thr Thr Glu Arg Cys Arg Gln Leu Val

165 170 175

Ala Ala Leu Gly Lys Arg Asp Val Val Cys Gln Ala Thr Pro Gly Phe

180 185 190

Ile Val Asn Arg Met Ala Arg Pro Tyr Tyr Leu Glu Gly Phe Arg Leu

195 200 205

Leu Glu Glu His Val Ala Arg Ala Pro Gln Ile Asp Arg Ala Leu Lys

210 215 220

Ala Gly Gly His Phe Arg Met Gly Pro Leu Glu Leu Thr Asp Phe Ile

225 230 235 240

Gly Gln Asp Ile Asn Tyr Gln Val Ser Arg Gln Ile Trp Gln Asp Met

245 250 255

Gln Tyr Asp Pro Arg Tyr Thr Pro Gly His Leu Gln Arg Ser Leu Val

260 265 270

Asp Ala Gly Leu Leu Gly Lys Lys Asn Gly Arg Ser Tyr Phe Ala Ala

275 280 285

Glu Glu Asn Ala Leu Pro Val Thr Ala Ala Ile Asp Ala Asp Val Glu

290 295 300

Thr Leu Arg Val Tyr Gly Glu His Pro Phe Phe Thr Leu Leu Gln Gln

305 310 315 320

Arg Ala Ala Leu Gln Trp Pro Arg Leu Arg Val Glu Gln Arg Pro Ala

325 330 335

Leu Pro Gly Leu Gly Pro Ala Val Gln Ile Asn Glu Ala Phe Thr Val

340 345 350

Ser Val Thr Asp Gly Arg Thr Ala Ser Gln Leu Ala Glu Leu Thr Ala

355 360 365

Ala Asp Val Phe Val Val Asp Val Ala Leu Asn Tyr Ala Asp Thr Ala

370 375 380

Tyr Leu Val Ala Ala His Asn Arg His Ala Ser Ala Ala Asn Lys Ala

385 390 395 400

Leu Phe Leu Arg Leu Leu His Thr Ala Ile Pro Gln Val Glu Phe Ile

405 410 415

Lys Asp Ser Pro Ala Leu Ile Val Ala Arg Val Leu Ser Ser Leu Ile

420 425 430

Asn Glu Ser Val Ile Met Val Glu Ser Gly Val Cys Ser Arg Glu Asp

435 440 445

Ile Asp Val Ala Ala Val Ala Gly Val Asn Tyr Ala Asp Gly Ile Phe

450 455 460

Gly Leu Leu Asp Ser Leu Gly Glu Lys Asn Val Arg Thr Thr Leu Ser

465 470 475 480

Asn Leu Ala Gln Leu Leu His Ala Ala Arg Tyr Ala Pro His Tyr Thr

485 490 495

Leu Leu His Ala Ala Gln Pro Ala Leu Thr Thr Thr Pro

500 505

<210> 56

<211> 509

<212> PRT

<213> Serratia marcescens 2880STDY5682930

<400> 56

Met Ala Glu Ser Asn Ala Ala Ile Gln Ser Ala Ala Ile Ile Gly Ala

1 5 10 15

Gly Thr Met Gly Arg Gly Ile Ala Tyr Leu Phe Ala Gln Lys Gly Ile

20 25 30

Arg Thr Val Leu Tyr Asn Arg Asn Gly Asn Thr Leu Asn Gln Ala Arg

35 40 45

Glu Tyr Ile Ala Gln Asp Leu Asn Lys Lys Val Glu Gln Gly Lys Ile

50 55 60

Ala Leu Gln Asp Lys Gly Ala Val Leu Ala Asn Leu Val Phe Thr Ser

65 70 75 80

Val Phe Glu Thr Ile Ala Asp Ser Asp Leu Val Ile Glu Thr Ile Ala

85 90 95

Glu Gln Glu Gln Thr Lys Leu Glu Val Leu Ala Ala Ile Ala Ala Ala

100 105 110

Val Lys Pro Asp Thr Leu Ile Ala Thr Asn Thr Ser Ser Leu Ser Leu

115 120 125

Asn Lys Leu Ala Thr Ala Val Thr His Ser Glu Arg Phe Ile Gly Leu

130 135 140

His Phe Phe Asn Pro Ala Pro Leu Met Lys Leu Ile Glu Ile Ile Pro

145 150 155 160

Ala Tyr Phe Thr Ala Arg Ala Thr Thr Glu Arg Cys Arg Gln Leu Val

165 170 175

Ala Ala Leu Gly Lys Arg Asp Val Val Cys Gln Ala Thr Pro Gly Phe

180 185 190

Ile Val Asn Arg Met Ala Arg Pro Tyr Tyr Leu Glu Gly Phe Arg Leu

195 200 205

Leu Glu Glu His Val Ala Arg Ala Pro Gln Ile Asp Arg Ala Leu Lys

210 215 220

Ala Gly Gly His Phe Arg Met Gly Pro Leu Glu Leu Thr Asp Phe Ile

225 230 235 240

Gly Gln Asp Ile Asn Tyr Gln Val Ser Arg Gln Ile Trp Gln Asp Met

245 250 255

Gln Tyr Asp Pro Arg Tyr Thr Pro Gly His Leu Gln Arg Ser Leu Val

260 265 270

Asp Ala Gly Leu Leu Gly Lys Lys Asn Gly Arg Ser Tyr Phe Ala Ala

275 280 285

Glu Glu Asn Ala Leu Pro Val Thr Ala Ala Thr Asp Ala Asp Val Glu

290 295 300

Thr Leu Arg Val Tyr Gly Glu His Pro Phe Phe Thr Leu Leu Gln Gln

305 310 315 320

Arg Ala Ala Leu Gln Trp Pro Arg Leu Arg Val Glu Gln Arg Pro Ala

325 330 335

Leu Pro Gly Leu Gly Pro Ala Val Gln Ile Asn Glu Ala Phe Thr Val

340 345 350

Ser Val Thr Asp Gly Arg Thr Ala Ser Gln Leu Ala Glu Leu Thr Ala

355 360 365

Ala Asp Ala Phe Val Val Asp Val Ala Leu Asn Tyr Ala Asp Thr Ala

370 375 380

Tyr Leu Val Ala Ala His Asn Arg His Ala Ser Ala Ala Asn Lys Ala

385 390 395 400

Leu Phe Leu Arg Leu Leu His Thr Ala Ile Pro Gln Val Glu Phe Ile

405 410 415

Lys Asp Ser Pro Ala Leu Ile Val Ala Arg Val Leu Ser Ser Leu Ile

420 425 430

Asn Glu Ser Val Ile Met Val Glu Ser Gly Val Cys Ser Arg Glu Asp

435 440 445

Ile Asn Val Ala Ala Val Ala Gly Val Asn Tyr Ala Asp Gly Ile Phe

450 455 460

Gly Leu Leu Asp Ser Leu Gly Glu Lys Asn Val Arg Thr Thr Leu Ser

465 470 475 480

Asn Leu Ala Gln Leu Leu His Ala Ala Arg Tyr Ala Pro His Tyr Thr

485 490 495

Leu Leu His Ala Ala Gln Pro Val Leu Thr Thr Thr Pro

500 505

<210> 57

<211> 509

<212> PRT

<213> Serratia marcescens 790

<400> 57

Met Ala Glu Ser Asn Ala Ala Ile Gln Ser Ala Ala Ile Ile Gly Ala

1 5 10 15

Gly Thr Met Gly Arg Gly Ile Ala Tyr Leu Phe Ala Gln Lys Gly Ile

20 25 30

Arg Thr Val Leu Tyr Asn Arg Asn Gly Asn Thr Leu Asn Gln Ala Arg

35 40 45

Glu Tyr Ile Ala Gln Asp Leu Asn Lys Lys Val Glu Gln Gly Lys Ile

50 55 60

Ala Leu Arg Asp Lys Gly Ala Val Leu Ala Asn Leu Val Phe Thr Ser

65 70 75 80

Val Phe Glu Thr Ile Ala Asp Ser Glu Leu Val Ile Glu Thr Ile Ala

85 90 95

Glu Gln Glu Gln Thr Lys Leu Glu Val Leu Ala Ala Ile Ala Ala Ala

100 105 110

Val Lys Pro Asp Thr Leu Ile Ala Thr Asn Thr Ser Ser Leu Ser Leu

115 120 125

Asn Lys Leu Ala Thr Ala Val Thr His Ser Glu Arg Phe Ile Gly Leu

130 135 140

His Phe Phe Asn Pro Ala Pro Leu Met Lys Leu Ile Glu Ile Ile Pro

145 150 155 160

Ala Tyr Phe Thr Ala Arg Ala Thr Thr Glu Arg Cys Arg Gln Leu Val

165 170 175

Ala Ala Leu Gly Lys Arg Asp Val Val Cys Gln Ala Thr Pro Gly Phe

180 185 190

Ile Val Asn Arg Met Ala Arg Pro Tyr Tyr Leu Glu Gly Phe Arg Leu

195 200 205

Leu Glu Glu His Val Ala Arg Ala Pro Gln Ile Asp Arg Ala Leu Lys

210 215 220

Ala Gly Gly His Phe Arg Met Gly Pro Leu Glu Leu Thr Asp Phe Ile

225 230 235 240

Gly Gln Asp Ile Asn Tyr Gln Val Ser Arg Gln Ile Trp Gln Asp Met

245 250 255

Gln Tyr Asp Pro Arg Tyr Thr Pro Gly His Leu Gln Arg Ser Leu Val

260 265 270

Asp Ala Gly Leu Leu Gly Lys Lys Asn Gly Arg Ser Tyr Phe Ala Ala

275 280 285

Glu Glu Asn Ala Leu Pro Val Thr Ala Ala Thr Asp Ala Asp Val Glu

290 295 300

Thr Leu Arg Val Tyr Gly Glu His Pro Phe Phe Thr Leu Leu Gln Gln

305 310 315 320

Arg Ala Ala Leu Gln Trp Pro Arg Leu Arg Val Glu Gln Arg Pro Ala

325 330 335

Leu Pro Gly Leu Gly Pro Ala Val Gln Thr Asn Glu Ala Phe Thr Val

340 345 350

Ser Val Thr Asp Gly Arg Thr Ala Asn Gln Leu Ala Glu Leu Thr Ala

355 360 365

Ala Asp Ala Phe Val Val Asp Val Ala Leu Asn Tyr Ala Asp Thr Ala

370 375 380

Tyr Leu Val Ala Ala His Asn Arg His Ala Ser Ala Ala Asn Lys Ala

385 390 395 400

Leu Phe Leu Arg Leu Leu His Thr Ala Ile Pro Gln Val Glu Phe Ile

405 410 415

Lys Asp Ser Pro Ala Leu Ile Val Ala Arg Val Leu Ser Ser Leu Ile

420 425 430

Asn Glu Ser Val Ile Met Val Glu Ser Gly Val Cys Ser Arg Glu Asp

435 440 445

Ile Asp Val Ala Ala Val Ala Gly Val Asn Tyr Ala Asp Gly Ile Phe

450 455 460

Gly Trp Leu Asp Ser Leu Gly Glu Lys Asn Val Arg Thr Thr Leu Ser

465 470 475 480

Asn Met Ala Gln Leu Leu His Ala Ala Arg Tyr Ala Pro His Tyr Thr

485 490 495

Leu Leu His Ala Ala Gln Pro Ala Leu Met Thr Thr Pro

500 505

<210> 58

<211> 509

<212> PRT

<213> Serratia marcescens UMH5

<400> 58

Met Ala Glu Ser Asn Ala Ala Ile Gln Ser Ala Ala Ile Ile Gly Ala

1 5 10 15

Gly Thr Met Gly Arg Gly Ile Ala Tyr Leu Phe Ala Gln Lys Gly Ile

20 25 30

Arg Thr Val Leu Tyr Asn Arg Asn Gly Asn Thr Leu Ser Gln Ala Arg

35 40 45

Asp Tyr Ile Glu Leu Asp Leu Asn Lys Lys Val Glu Gln Gly Lys Ile

50 55 60

Ala Leu Gln Asp Lys Gly Ala Val Leu Ala Asn Leu Val Phe Thr Ser

65 70 75 80

Val Phe Glu Thr Ile Ala Asp Ser Glu Leu Val Ile Glu Thr Ile Ala

85 90 95

Glu Gln Glu Gln Thr Lys Leu Glu Val Leu Ala Thr Ile Ala Ala Ala

100 105 110

Val Lys Pro Asp Thr Leu Ile Ala Thr Asn Thr Ser Ser Leu Ser Leu

115 120 125

Asn Lys Leu Ala Thr Ala Val Thr His Ser Glu Arg Phe Ile Gly Leu

130 135 140

His Phe Phe Asn Pro Ala Pro Leu Met Lys Leu Ile Glu Ile Ile Pro

145 150 155 160

Ala Tyr Cys Thr Ala His Ala Thr Thr Glu Arg Cys Arg Gln Leu Val

165 170 175

Ala Ala Leu Gly Lys Arg Asp Val Val Cys Gln Ala Thr Pro Gly Phe

180 185 190

Ile Val Asn Arg Met Ala Arg Pro Tyr Tyr Leu Glu Gly Phe Arg Leu

195 200 205

Leu Glu Glu His Val Ala Arg Ala Pro Gln Ile Asp Arg Ala Leu Lys

210 215 220

Ala Gly Gly His Phe Arg Met Gly Pro Leu Glu Leu Thr Asp Phe Ile

225 230 235 240

Gly Gln Asp Ile Asn Tyr Gln Val Ser Arg Gln Ile Trp Gln Asp Met

245 250 255

Gln Tyr Asp Ala Arg Tyr Thr Pro Gly His Leu Gln Arg Ser Leu Val

260 265 270

Asp Ala Gly Leu Leu Gly Lys Lys Asn Gly Arg Ser Tyr Phe Ala Val

275 280 285

Glu Glu Asn Ala Pro Pro Val Met Ala Ala Thr Asp Ala Asp Ile Glu

290 295 300

Thr Leu His Val Tyr Gly Glu His Pro Phe Phe Thr Leu Leu Gln Gln

305 310 315 320

Arg Ala Ala Leu Gln Trp Pro Arg Leu Arg Val Glu Gln Arg Gln Ala

325 330 335

Leu Pro Gly Leu Gly Pro Ala Val Arg Ile Asn Asp Ala Phe Thr Ile

340 345 350

Ser Ile Thr Asp Gly Arg Thr Ala Asn Gln Leu Ala Glu Gln Thr Ala

355 360 365

Ala Asp Ala Phe Val Val Asp Val Ala Leu Asn Tyr Ala Asp Thr Ala

370 375 380

Tyr Leu Val Ala Ala His Ser Arg His Ala Ser Gly Ala Asn Lys Ala

385 390 395 400

Leu Phe Leu Arg Leu Leu His Thr Ala Ile Pro Gln Val Glu Phe Ile

405 410 415

Lys Asp Ser Pro Ala Leu Ile Val Ala Arg Val Leu Ser Ser Leu Ile

420 425 430

Asn Glu Ser Val Ile Met Val Glu Ser Gly Val Cys Ser Arg Glu Asp

435 440 445

Ile Asp Val Ala Ala Val Ala Gly Val Asn Tyr Ala Asp Gly Ile Phe

450 455 460

Gly Trp Leu Asp Ser Leu Gly Glu Lys Asn Val Arg Thr Thr Leu Ser

465 470 475 480

Asn Leu Ala Gln Leu Leu His Ala Ala Arg Tyr Ala Pro His Tyr Thr

485 490 495

Leu Leu His Ala Ala Gln Pro Ala Leu Thr Thr Thr Pro

500 505

<210> 59

<211> 509

<212> PRT

<213> Serratia marcescens 2880STDY5682988

<400> 59

Met Ala Glu Ser Asn Ala Ala Ile Gln Ser Ala Ala Ile Ile Gly Ala

1 5 10 15

Gly Thr Met Gly Arg Gly Ile Ala Tyr Leu Phe Ala Gln Lys Gly Ile

20 25 30

Arg Thr Val Leu Tyr Asn Arg Asn Gly Asn Thr Leu Ser Gln Ala Arg

35 40 45

Asp Tyr Ile Glu Leu Asp Leu Asn Lys Lys Val Glu Gln Gly Lys Ile

50 55 60

Ala Leu Gln Asp Lys Gly Ala Val Leu Ala Asn Leu Val Phe Thr Ser

65 70 75 80

Val Phe Glu Thr Ile Ala Asp Ser Glu Leu Val Ile Glu Thr Ile Ala

85 90 95

Glu Gln Glu Gln Thr Lys Leu Glu Val Leu Ala Thr Ile Ala Ala Ala

100 105 110

Val Lys Pro Asp Thr Leu Ile Ala Thr Asn Thr Ser Ser Leu Ser Leu

115 120 125

Asn Lys Leu Ala Thr Ala Val Thr His Ser Glu Arg Phe Ile Gly Leu

130 135 140

His Phe Phe Asn Pro Ala Pro Leu Met Lys Leu Ile Glu Ile Ile Pro

145 150 155 160

Ala Tyr Cys Thr Ala His Ala Thr Thr Glu Arg Cys Arg Gln Leu Val

165 170 175

Ala Ala Leu Gly Lys Arg Asp Val Val Cys Gln Ala Thr Pro Gly Phe

180 185 190

Ile Val Asn Arg Met Ala Arg Pro Tyr Tyr Leu Glu Gly Phe Arg Leu

195 200 205

Leu Glu Glu His Val Ala Arg Ala Pro Gln Ile Asp Arg Ala Leu Lys

210 215 220

Ala Gly Gly His Phe Arg Met Gly Pro Leu Glu Leu Thr Asp Phe Ile

225 230 235 240

Gly Gln Asp Ile Asn Tyr Gln Val Ser Arg Gln Ile Trp Gln Asp Met

245 250 255

Gln Tyr Asp Ala Arg Tyr Thr Pro Gly His Leu Gln Arg Ser Leu Val

260 265 270

Asp Ala Gly Leu Leu Gly Lys Lys Asn Gly Arg Ser Tyr Phe Ala Val

275 280 285

Glu Glu Asn Ala Pro Pro Val Met Ala Ala Thr Asp Ala Asp Ile Glu

290 295 300

Thr Leu His Val Tyr Gly Glu His Pro Phe Phe Thr Leu Leu Gln Gln

305 310 315 320

Arg Ala Ala Leu Gln Trp Pro Arg Leu Arg Val Glu Gln Arg Gln Ala

325 330 335

Leu Pro Gly Leu Gly Pro Ala Val Arg Ile Asn Asp Ala Phe Thr Ile

340 345 350

Ser Ile Thr Asp Gly Arg Thr Ala Asn Gln Leu Ala Glu Gln Thr Ala

355 360 365

Thr Asp Ala Phe Val Val Asp Val Ala Leu Asn Tyr Ala Asp Thr Ala

370 375 380

Tyr Leu Val Ala Ala His Ser Arg His Ala Ser Gly Ala Asn Lys Ala

385 390 395 400

Leu Phe Leu Arg Leu Leu His Thr Ala Ile Pro Gln Val Glu Phe Ile

405 410 415

Lys Asp Ser Pro Ala Leu Ile Val Ala Arg Val Leu Ser Ser Leu Ile

420 425 430

Asn Glu Ser Val Ile Met Val Glu Ser Gly Val Cys Ser Arg Glu Asp

435 440 445

Ile Asp Val Ala Ala Val Ala Gly Val Asn Tyr Ala Asp Gly Ile Phe

450 455 460

Gly Trp Leu Asp Ser Leu Gly Glu Lys Asn Val Arg Thr Thr Leu Ser

465 470 475 480

Asn Leu Ala Gln Leu Leu His Ala Ala Arg Tyr Ala Pro His Tyr Thr

485 490 495

Leu Leu His Ala Ala Gln Pro Ala Leu Thr Thr Thr Pro

500 505

<210> 60

<211> 509

<212> PRT

<213> Serratia marcescens 945154301

<400> 60

Met Ala Glu Ser Asn Ala Ala Ile Gln Ser Ala Ala Ile Ile Gly Ala

1 5 10 15

Gly Thr Met Gly Arg Gly Ile Ala Tyr Leu Phe Ala Gln Lys Gly Ile

20 25 30

Arg Thr Val Leu Tyr Asn Arg Asn Gly Asn Thr Leu Asn Gln Ala Arg

35 40 45

Asp Tyr Ile Glu Leu Asp Leu Asn Lys Lys Val Glu Gln Gly Lys Ile

50 55 60

Ala Leu Gln Asp Lys Gly Ala Val Leu Ala Asn Leu Val Phe Thr Ser

65 70 75 80

Val Phe Glu Thr Ile Ala Asp Ser Glu Leu Val Ile Glu Thr Ile Ala

85 90 95

Glu Gln Glu Gln Thr Lys Leu Glu Val Leu Ala Ala Ile Ala Ala Ala

100 105 110

Val Lys Pro Asp Thr Leu Ile Ala Thr Asn Thr Ser Ser Leu Ser Leu

115 120 125

Asn Lys Leu Ala Thr Ala Val Thr His Ser Glu Arg Phe Ile Gly Leu

130 135 140

His Phe Phe Asn Pro Ala Pro Leu Met Lys Leu Ile Glu Ile Ile Pro

145 150 155 160

Ala Tyr Phe Thr Ala His Ala Thr Thr Glu Arg Cys Arg Gln Leu Val

165 170 175

Ala Ala Leu Gly Lys Arg Asp Val Val Cys Gln Ala Thr Pro Gly Phe

180 185 190

Ile Val Asn Arg Met Ala Arg Pro Tyr Tyr Leu Glu Gly Phe Arg Leu

195 200 205

Leu Glu Glu His Val Ala Arg Ala Pro Gln Ile Asp Arg Ala Leu Lys

210 215 220

Ala Gly Gly His Phe Arg Met Gly Pro Leu Glu Leu Thr Asp Phe Ile

225 230 235 240

Gly Gln Asp Ile Asn Tyr Gln Val Ser Arg Gln Ile Trp Gln Asp Met

245 250 255

Gln Tyr Asp Ala Arg Tyr Thr Pro Gly His Leu Gln Arg Ser Leu Val

260 265 270

Asp Ala Gly Leu Leu Gly Lys Lys Asn Gly Arg Ser Tyr Phe Ala Ala

275 280 285

Glu Glu Asn Ala Pro Pro Val Met Ala Ala Thr Asp Ala Asp Ile Glu

290 295 300

Thr Leu His Val Tyr Gly Glu His Pro Phe Phe Thr Leu Leu Gln Gln

305 310 315 320

Arg Ala Ala Leu Gln Trp Pro Gln Leu Arg Val Glu Gln Arg Pro Ala

325 330 335

Leu Pro Gly Leu Gly Pro Ala Val Arg Ile Asn Asp Ala Phe Ser Val

340 345 350

Ser Ile Thr Asp Gly Arg Thr Ala Asn Gln Leu Ala Glu Gln Thr Ala

355 360 365

Ala Asp Ala Phe Val Val Asp Val Ala Leu Asn Tyr Ala Asp Thr Ala

370 375 380

Tyr Leu Val Ala Ala His Ser Arg His Ala Ser Ala Ala Asn Lys Ala

385 390 395 400

Leu Phe Leu Arg Leu Leu His Thr Ala Ile Pro Gln Val Glu Phe Ile

405 410 415

Lys Asp Ser Pro Ala Leu Ile Val Ala Arg Val Leu Ser Ser Leu Ile

420 425 430

Asn Glu Ser Val Ile Met Val Glu Ser Gly Val Cys Ser Arg Glu Asp

435 440 445

Ile Asp Val Ala Ala Val Ala Gly Val Asn Tyr Ala Asp Gly Ile Phe

450 455 460

Gly Trp Leu Asp Ser Leu Gly Glu Lys Asn Val Arg Thr Thr Leu Ser

465 470 475 480

Asn Leu Ala Gln Leu Leu His Ala Ala Arg Tyr Ala Pro His Tyr Thr

485 490 495

Leu Leu His Ala Ala Gln Pro Ala Leu Thr Thr Thr Pro

500 505

<210> 61

<211> 509

<212> PRT

<213> Serratia marcescens at10508

<400> 61

Met Ala Glu Ser Asn Ala Ala Ile Gln Ser Ala Ala Ile Ile Gly Ala

1 5 10 15

Gly Thr Met Gly Arg Gly Ile Ala Tyr Leu Phe Ala Gln Lys Gly Ile

20 25 30

Arg Thr Val Leu Tyr Asn Arg Asn Gly Asn Thr Leu Asn Gln Ala Arg

35 40 45

Asp Tyr Ile Glu Leu Asp Leu Asn Lys Lys Val Glu Gln Gly Lys Ile

50 55 60

Ala Leu Gln Asp Lys Gly Ala Val Leu Ala Asn Leu Val Phe Thr Ser

65 70 75 80

Val Phe Glu Thr Ile Ala Asp Ser Glu Leu Val Ile Glu Thr Ile Ala

85 90 95

Glu Gln Glu Gln Thr Lys Leu Glu Val Leu Ala Ala Ile Ala Ala Ala

100 105 110

Val Lys Pro Asp Thr Leu Ile Ala Thr Asn Thr Ser Ser Leu Ser Leu

115 120 125

Asn Lys Leu Ala Thr Ala Val Thr His Ser Glu Arg Phe Ile Gly Leu

130 135 140

His Phe Phe Asn Pro Ala Pro Leu Met Lys Leu Ile Glu Ile Ile Pro

145 150 155 160

Ala Tyr Phe Thr Ala His Ala Thr Thr Glu Arg Cys Arg Gln Leu Val

165 170 175

Thr Ala Leu Gly Lys Arg Asp Val Val Cys Gln Ala Thr Pro Gly Phe

180 185 190

Ile Val Asn Arg Met Ala Arg Pro Tyr Tyr Leu Glu Gly Phe Arg Leu

195 200 205

Leu Glu Glu His Val Ala Arg Ala Pro Gln Ile Asp Arg Ala Leu Lys

210 215 220

Ala Gly Gly His Phe Arg Met Gly Pro Leu Glu Leu Thr Asp Phe Ile

225 230 235 240

Gly Gln Asp Ile Asn Tyr Gln Val Ser Arg Gln Ile Trp Gln Asp Met

245 250 255

Gln Tyr Asp Ala Arg Tyr Thr Pro Gly His Leu Gln Arg Ser Leu Val

260 265 270

Asp Ala Gly Leu Leu Gly Lys Lys Asn Gly Arg Ser Tyr Phe Ala Val

275 280 285

Glu Glu Asn Ala Pro Pro Val Met Ala Ala Thr Asp Ala Asp Ile Glu

290 295 300

Thr Leu His Val Tyr Gly Glu His Pro Phe Phe Thr Leu Leu Gln Gln

305 310 315 320

Arg Ala Ala Leu Gln Trp Pro Gln Leu Arg Val Glu Gln Arg Pro Ala

325 330 335

Leu Pro Gly Leu Gly Pro Ala Val Arg Ile Asn Asp Ala Phe Thr Val

340 345 350

Ser Ile Thr Asp Gly Arg Thr Ala Asn Gln Leu Ala Glu Gln Thr Ala

355 360 365

Ala Asp Ala Phe Val Val Asp Val Ala Leu Asn Tyr Ala Asp Thr Ala

370 375 380

Tyr Leu Val Ala Ala His Ser Arg His Ala Ser Ala Ala Asn Lys Ala

385 390 395 400

Leu Phe Leu Arg Leu Leu His Thr Ala Ile Pro Gln Val Glu Phe Ile

405 410 415

Lys Asp Ser Pro Ala Leu Ile Val Ala Arg Val Leu Ser Ser Leu Ile

420 425 430

Asn Glu Ser Val Ile Met Val Glu Ser Gly Val Cys Ser Arg Glu Asp

435 440 445

Ile Asp Val Ala Ala Val Ala Gly Val Asn Tyr Ala Asp Gly Ile Phe

450 455 460

Gly Trp Leu Asp Ser Leu Gly Glu Lys Asn Val Arg Thr Thr Leu Ser

465 470 475 480

Asn Leu Ala Gln Leu Leu His Ala Ala Arg Tyr Ala Pro His Tyr Thr

485 490 495

Leu Leu His Ala Ala Gln Pro Ala Leu Thr Thr Thr Pro

500 505

<210> 62

<211> 509

<212> PRT

<213> Serratia marcescens ML2637

<400> 62

Met Ala Glu Ser Asn Ala Ala Ile Gln Ser Ala Ala Ile Ile Gly Ala

1 5 10 15

Gly Thr Met Gly Arg Gly Ile Ala Tyr Leu Phe Ala Gln Lys Gly Ile

20 25 30

Arg Thr Val Leu Tyr Asn Arg Asn Gly Asn Thr Leu Asn Gln Ala Arg

35 40 45

Asp Tyr Ile Glu Leu Asp Leu Asn Lys Lys Val Glu Gln Gly Lys Ile

50 55 60

Ala Leu Gln Asp Lys Gly Ala Val Leu Ala Asn Leu Val Phe Thr Ser

65 70 75 80

Val Phe Glu Thr Ile Ala Asp Ser Glu Leu Val Ile Glu Thr Ile Ala

85 90 95

Glu Gln Glu Gln Thr Lys Leu Glu Val Leu Ala Ala Ile Ala Ala Ala

100 105 110

Val Lys Pro Asp Thr Leu Ile Ala Thr Asn Thr Ser Ser Leu Ser Leu

115 120 125

Asn Lys Leu Ala Thr Ala Val Thr His Ser Glu Arg Phe Ile Gly Leu

130 135 140

His Phe Phe Asn Pro Ala Pro Leu Met Lys Leu Ile Glu Ile Ile Pro

145 150 155 160

Ala Tyr Phe Thr Ala His Ala Thr Thr Glu Arg Cys Arg Gln Leu Val

165 170 175

Thr Ala Leu Gly Lys Arg Asp Val Val Cys Gln Ala Thr Pro Gly Phe

180 185 190

Ile Val Asn Arg Met Ala Arg Pro Tyr Tyr Leu Glu Gly Phe Arg Leu

195 200 205

Leu Glu Glu His Val Ala Arg Ala Pro Gln Ile Asp Arg Ala Leu Lys

210 215 220

Ala Gly Gly His Phe Arg Met Gly Pro Leu Glu Leu Thr Asp Phe Ile

225 230 235 240

Gly Gln Asp Ile Asn Tyr Gln Val Ser Arg Gln Ile Trp Gln Asp Met

245 250 255

Gln Tyr Asp Ala Arg Tyr Thr Pro Gly His Leu Gln Arg Ser Leu Val

260 265 270

Asp Ala Gly Leu Leu Gly Lys Lys Asn Gly Arg Ser Tyr Phe Ala Val

275 280 285

Glu Glu Asn Ala Pro Pro Val Met Ala Ala Thr Asp Ala Asp Ile Glu

290 295 300

Thr Leu His Val Tyr Gly Glu His Pro Phe Phe Thr Leu Leu Gln Gln

305 310 315 320

Arg Ala Ala Leu Gln Trp Pro Gln Leu Arg Val Glu Gln Arg Pro Ala

325 330 335

Leu Pro Gly Leu Gly Pro Ala Val Arg Ile Asn Asp Ala Phe Thr Val

340 345 350

Ser Ile Thr Asp Gly Arg Thr Ala Asn Gln Leu Ala Glu Gln Thr Ala

355 360 365

Ala Asp Ala Phe Val Val Asp Val Ala Leu Asn Tyr Ala Asp Thr Ala

370 375 380

Tyr Leu Val Ala Ala His Ser Arg His Ala Ser Ala Ala Asn Lys Ala

385 390 395 400

Leu Phe Leu Arg Leu Leu His Thr Ala Ile Pro Gln Val Glu Phe Ile

405 410 415

Lys Asp Ser Pro Ala Leu Ile Val Ala Arg Val Leu Ser Ser Leu Ile

420 425 430

Asn Glu Ser Val Ile Met Val Glu Ser Gly Val Cys Ser Arg Glu Asp

435 440 445

Ile Asp Val Ala Ala Val Ala Gly Val Asn Tyr Ala Asp Gly Ile Phe

450 455 460

Gly Trp Leu Asp Ser Leu Gly Glu Lys Asn Val Arg Thr Thr Leu Ser

465 470 475 480

Asn Leu Ala Gln Leu Leu His Ala Ala Arg Tyr Ala Pro His Tyr Thr

485 490 495

Leu Leu His Ala Val Gln Pro Ala Leu Thr Thr Thr Pro

500 505

<210> 63

<211> 509

<212> PRT

<213> Serratia marcescens SM1978

<400> 63

Met Ala Glu Ser Asn Ala Ala Ile Gln Ser Ala Ala Ile Ile Gly Ala

1 5 10 15

Gly Thr Met Gly Arg Gly Ile Ala Tyr Leu Phe Ala Gln Lys Gly Ile

20 25 30

Arg Thr Val Leu Tyr Asn Arg Asn Gly Asn Thr Leu Asn Gln Ala Arg

35 40 45

Asp Tyr Ile Glu Leu Asp Leu Asn Lys Lys Val Glu Gln Gly Lys Ile

50 55 60

Ala Leu Gln Asp Lys Gly Ala Val Leu Ala Asn Leu Val Phe Thr Ser

65 70 75 80

Val Phe Glu Thr Ile Ala Asp Ser Glu Leu Val Ile Glu Thr Ile Ala

85 90 95

Glu Gln Glu Gln Thr Lys Leu Glu Val Leu Ala Ala Ile Ala Ala Ala

100 105 110

Val Lys Pro Asp Thr Leu Ile Ala Thr Asn Thr Ser Ser Leu Ser Leu

115 120 125

Asn Lys Leu Ala Thr Ala Val Thr His Ser Glu Arg Phe Ile Gly Leu

130 135 140

His Phe Phe Asn Pro Ala Pro Leu Met Lys Leu Ile Glu Ile Ile Pro

145 150 155 160

Ala Tyr Phe Thr Ala His Ala Thr Thr Glu Arg Cys Arg Gln Leu Val

165 170 175

Thr Ala Leu Gly Lys Arg Asp Val Val Cys Gln Ala Thr Pro Gly Phe

180 185 190

Ile Val Asn Arg Met Ala Arg Pro Tyr Tyr Leu Glu Gly Phe Arg Leu

195 200 205

Leu Glu Glu His Val Ala Arg Ala Pro Gln Ile Asp Arg Ala Leu Lys

210 215 220

Ala Gly Gly His Phe Arg Met Gly Pro Leu Glu Leu Thr Asp Phe Ile

225 230 235 240

Gly Gln Asp Ile Asn Tyr Gln Val Ser Arg Gln Ile Trp Gln Asp Met

245 250 255

Gln Tyr Asp Ala Arg Tyr Thr Pro Gly His Leu Gln Arg Ser Leu Val

260 265 270

Asp Ala Gly Leu Leu Gly Lys Lys Asn Gly Arg Ser Tyr Phe Ala Ala

275 280 285

Glu Glu Asn Ala Pro Pro Val Met Ala Ser Thr Asp Ala Asp Ile Glu

290 295 300

Thr Leu His Val Tyr Gly Glu His Pro Phe Phe Thr Leu Leu Gln Gln

305 310 315 320

Arg Ala Ala Leu Gln Trp Pro Gln Leu Arg Val Glu Gln Arg Pro Ala

325 330 335

Leu Pro Gly Leu Gly Pro Ala Val Arg Ile Asn Asp Ala Phe Ser Val

340 345 350

Ser Ile Thr Asp Gly Arg Thr Ala Asn Gln Leu Ala Glu Gln Thr Thr

355 360 365

Ala Asp Ala Phe Val Val Asp Val Ala Leu Asn Tyr Ala Asp Thr Ala

370 375 380

Tyr Leu Val Ala Ala His Ser Arg His Ala Ser Ala Ala Asn Lys Ala

385 390 395 400

Leu Phe Leu Arg Leu Leu His Thr Ala Ile Pro Gln Val Glu Phe Ile

405 410 415

Lys Asp Ser Pro Ala Leu Ile Val Ala Arg Val Leu Ser Ser Leu Ile

420 425 430

Asn Glu Ser Val Ile Met Val Glu Ser Gly Val Cys Ser Arg Glu Asp

435 440 445

Ile Asp Val Ala Ala Val Ala Gly Val Asn Tyr Ala Asp Gly Ile Phe

450 455 460

Gly Trp Leu Asp Ser Leu Gly Glu Lys Asn Val Arg Thr Thr Leu Ser

465 470 475 480

Asn Leu Ala Gln Leu Leu His Ala Ala Arg Tyr Ala Pro His Tyr Thr

485 490 495

Leu Leu His Ala Ala Gln Pro Ala Leu Thr Thr Thr Pro

500 505

<210> 64

<211> 509

<212> PRT

<213> Serratia marcescens PWN146

<400> 64

Met Ala Glu Ser Asn Ala Ala Ile Gln Ser Ala Ala Ile Ile Gly Ala

1 5 10 15

Gly Thr Met Gly Arg Gly Ile Ala Tyr Leu Phe Ala Gln Lys Gly Ile

20 25 30

Arg Thr Val Leu Tyr Asn Arg Asn Gly Asn Thr Leu Asn Gln Ala Arg

35 40 45

Asp Tyr Ile Glu Leu Asp Leu Asn Lys Lys Val Glu Gln Gly Lys Ile

50 55 60

Ala Leu Gln Asp Lys Gly Ala Val Leu Ala Asn Leu Val Phe Thr Ser

65 70 75 80

Val Phe Glu Thr Ile Ala Asp Ser Glu Leu Val Ile Glu Thr Ile Ala

85 90 95

Glu Gln Glu Gln Thr Lys Leu Glu Val Leu Ala Thr Ile Ala Ala Ala

100 105 110

Val Lys Pro Asp Thr Leu Ile Ala Thr Asn Thr Ser Ser Leu Ser Leu

115 120 125

Asn Lys Leu Ala Thr Ala Val Thr His Ser Glu Arg Phe Ile Gly Leu

130 135 140

His Phe Phe Asn Pro Ala Pro Leu Met Lys Leu Ile Glu Ile Ile Pro

145 150 155 160

Ala Tyr Phe Thr Ala His Ala Thr Thr Glu Arg Cys Arg Gln Leu Val

165 170 175

Ala Ala Leu Gly Lys Arg Asp Val Val Cys Gln Ala Thr Pro Gly Phe

180 185 190

Ile Val Asn Arg Met Ala Arg Pro Tyr Tyr Leu Glu Gly Phe Arg Leu

195 200 205

Leu Glu Glu His Val Ala Arg Ala Pro Gln Ile Asp Arg Ala Leu Lys

210 215 220

Ala Gly Gly His Phe Arg Met Gly Pro Leu Glu Leu Thr Asp Phe Ile

225 230 235 240

Gly Gln Asp Ile Asn Tyr Gln Val Ser Arg Gln Ile Trp Gln Asp Met

245 250 255

Gln Tyr Asp Ala Arg Tyr Thr Pro Gly His Leu Gln Arg Ser Leu Val

260 265 270

Asp Ala Gly Leu Leu Gly Lys Lys Asn Gly Arg Ser Tyr Phe Ala Val

275 280 285

Glu Glu Asn Ala Pro Pro Val Met Ala Ala Thr Asp Ala Asp Ile Glu

290 295 300

Thr Leu His Val Cys Gly Glu His Pro Phe Phe Thr Leu Leu Gln Gln

305 310 315 320

Arg Ala Ala Leu Gln Trp Pro Arg Leu Arg Val Glu Gln Arg Pro Ala

325 330 335

Leu Pro Gly Leu Gly Pro Ala Val Trp Ile Asn Asp Ala Phe Thr Val

340 345 350

Ser Ile Thr Asp Gly Arg Thr Ala Asn Gln Leu Ala Glu Gln Thr Ala

355 360 365

Ala Asp Ala Phe Val Val Asp Val Ala Leu Asn Tyr Ala Asp Thr Ala

370 375 380

Tyr Leu Val Ala Ala His Ser Arg His Ala Ser Ala Ala Asn Lys Ala

385 390 395 400

Leu Phe Leu Arg Leu Leu His Thr Ala Ile Pro Gln Val Glu Phe Ile

405 410 415

Lys Asp Ser Pro Ala Leu Ile Val Ala Arg Val Leu Ser Ser Leu Ile

420 425 430

Asn Glu Ser Val Ile Met Val Glu Ser Gly Val Cys Ser Arg Glu Asp

435 440 445

Ile Asp Val Ala Ala Val Ala Gly Val Asn Tyr Ala Asp Gly Ile Phe

450 455 460

Gly Trp Leu Asp Ser Leu Gly Glu Lys Asn Val Arg Ala Thr Leu Ser

465 470 475 480

Asn Leu Ala Gln Leu Leu His Ala Ala Arg Tyr Ala Pro His Tyr Thr

485 490 495

Leu Leu His Ala Ala Gln Pro Ala Leu Thr Thr Thr Pro

500 505

<210> 65

<211> 509

<212> PRT

<213> Serratia marcescens H1q

<400> 65

Met Ala Glu Arg Asn Ala Ala Ile Gln Ser Ala Ala Ile Ile Gly Ala

1 5 10 15

Gly Thr Met Gly Arg Gly Ile Ala Tyr Leu Phe Ala Gln Lys Gly Ile

20 25 30

Arg Thr Val Leu Tyr Asn Arg Asn Gly Asn Thr Leu Ile Gln Ala Arg

35 40 45

Glu Tyr Ile Val Gln Asp Leu Asp Lys Lys Val Glu Gln Gly Arg Leu

50 55 60

Ala Pro Gln Asp Lys Asp Ala Val Leu Ala Asn Leu Gln Phe Ser Ser

65 70 75 80

Val Phe Glu Ala Ile Val Asp Ser Asp Leu Val Leu Glu Thr Ile Ala

85 90 95

Glu Gln Glu Gln Ala Lys Leu Glu Val Leu Ala Ala Ile Ala Ala Ala

100 105 110

Val Lys Pro Asp Thr Leu Ile Ala Thr Asn Thr Ser Ser Leu Ser Leu

115 120 125

Asn Lys Leu Ala Thr Ala Val Thr His Ser Glu Arg Phe Ile Gly Leu

130 135 140

His Phe Phe Asn Pro Ala Pro Leu Met Lys Leu Ile Glu Ile Ile Pro

145 150 155 160

Ala Tyr Phe Thr Ala Gln Ala Thr Thr Glu Thr Cys Arg Gln Leu Val

165 170 175

Ala Ala Leu Gly Lys Arg Asp Val Val Cys Gln Ala Thr Pro Gly Phe

180 185 190

Ile Val Asn Arg Met Ala Arg Pro Tyr Tyr Leu Glu Gly Phe Arg Leu

195 200 205

Leu Glu Glu His Val Ala Arg Ala Pro Gln Ile Asp Arg Ala Leu Lys

210 215 220

Ala Gly Gly His Phe Arg Met Gly Pro Leu Glu Leu Thr Asp Phe Ile

225 230 235 240

Gly Gln Asp Ile Asn Tyr Gln Val Ser Arg Gln Ile Trp Gln Asp Met

245 250 255

Gln Tyr Asp Pro Arg Tyr Thr Pro Gly His Leu Gln Arg Ser Leu Val

260 265 270

Asp Ala Gly Leu Leu Gly Lys Lys Asn Gly Arg Ser Tyr Phe Ser Ala

275 280 285

Glu Glu Thr Ala Pro Pro Val Glu Ala Ala Val Glu Ala Asp Val Glu

290 295 300

Thr Leu Arg Ile Tyr Gly Glu His Pro Leu Phe Thr Leu Leu Gln Gln

305 310 315 320

Arg Ala Ala Leu Gln Trp Pro Gln Leu Arg Val Glu Gln Arg Pro Ala

325 330 335

Leu Ser Gly Leu Gly Ala Ala Ile Gln Val Asn Asp Ala Phe Thr Val

340 345 350

Ser Ile Thr Asp Gly Arg Thr Ala Asn Gln Leu Ala Glu Gln Thr Ala

355 360 365

Ala Asp Ala Phe Val Val Asp Val Ala Leu Asn Tyr Ala Asp Thr Ala

370 375 380

Tyr Leu Val Ala Ala His Ser Arg His Ala Ser Ala Ala Asn Lys Ala

385 390 395 400

Leu Phe Leu Arg Leu Leu His Thr Ala Leu Pro Gln Val Glu Phe Ile

405 410 415

Lys Asp Ser Pro Ala Leu Ile Val Ala Arg Val Leu Ser Ser Leu Ile

420 425 430

Asn Glu Ser Val Ile Met Val Glu Ser Gly Val Cys Ser Arg Glu Asp

435 440 445

Ile Asp Val Ala Ala Val Ala Gly Val Asn Tyr Ala Asp Gly Ile Phe

450 455 460

Gly Trp Leu Thr Arg Leu Gly Glu Lys Asn Val Arg Thr Thr Leu Ser

465 470 475 480

Asn Leu Ala Gln Leu Leu His Ala Ala Arg Tyr Ala Pro His Tyr Thr

485 490 495

Leu Leu His Ala Ala Gln Pro Ala Leu Thr Thr Thr Pro

500 505

<210> 66

<211> 509

<212> PRT

<213> Serratia marcescens UMH6

<400> 66

Met Ala Glu Ser Asn Ala Ala Ile Gln Ser Ala Ala Ile Ile Gly Ala

1 5 10 15

Gly Thr Met Gly Arg Gly Ile Ala Tyr Leu Phe Ala Gln Lys Ser Ile

20 25 30

Arg Thr Val Leu Tyr Asn Arg Asn Gly Asn Thr Leu Asn Gln Ala Arg

35 40 45

Asp Ala Ile Val Gln Asp Leu Asn Lys Lys Val Glu Gln Gly Lys Leu

50 55 60

Ala Leu Gln Asp Lys Asp Ala Val Leu Ala Asn Leu Thr Phe Ser Ala

65 70 75 80

Glu Phe Gly Ala Ile Ala Asp Ser Asp Leu Val Ile Glu Thr Ile Ala

85 90 95

Glu His Glu Gln Ala Lys Leu Glu Val Leu Ala Ala Ile Ala Ala Thr

100 105 110

Val Lys Pro Asp Thr Leu Ile Ala Thr Asn Thr Ser Ser Leu Ser Leu

115 120 125

Asn Lys Leu Ala Thr Ala Val Thr His Ser Glu Arg Phe Ile Gly Leu

130 135 140

His Phe Phe Asn Pro Ala Pro Leu Met Lys Leu Ile Glu Ile Ile Pro

145 150 155 160

Ala Tyr Phe Thr Ala Gln Val Thr Thr Glu Arg Cys Arg Gln Leu Val

165 170 175

Ala Ala Leu Gly Lys Arg Asp Val Val Cys Gln Ala Thr Pro Gly Phe

180 185 190

Ile Val Asn Arg Met Ala Arg Pro Tyr Tyr Leu Glu Gly Phe Arg Leu

195 200 205

Leu Glu Glu His Val Ala Arg Ala Pro Gln Ile Asp Arg Ala Leu Lys

210 215 220

Ala Gly Gly His Phe Arg Met Gly Pro Leu Glu Leu Thr Asp Phe Ile

225 230 235 240

Gly Gln Asp Ile Asn Tyr Gln Val Ser Arg Gln Ile Trp Gln Asp Met

245 250 255

Gln Tyr Asp Pro Arg Tyr Thr Pro Gly His Leu Gln Arg Ser Leu Val

260 265 270

Asp Ala Gly Leu Leu Gly Lys Lys Asn Gly Arg Ser Tyr Phe Ser Ala

275 280 285

Glu Glu Ser Pro Pro Pro Leu Ala Ala Ala Val Asp Ala Glu Val Glu

290 295 300

Thr Leu Arg Ile Tyr Gly Glu His Pro Leu Phe Thr Leu Leu Gln Gln

305 310 315 320

Arg Ala Ala Leu Gln Trp Pro Arg Leu Arg Val Glu Gln Arg Pro Thr

325 330 335

Leu Pro Gly Leu Gly Ala Ala Ile Gln Val Asn Asp Ala Phe Thr Val

340 345 350

Ser Val Thr Asp Gly Arg Thr Ala Asn Gln Leu Ala Glu Gln Thr Ala

355 360 365

Ala Asp Ala Phe Val Val Asp Val Ala Leu Asn Tyr Gly Asp Thr Ala

370 375 380

Tyr Leu Val Ala Ala His Ser Arg His Ala Ser Ala Ala Asn Lys Ala

385 390 395 400

Leu Phe Leu Arg Leu Leu His Thr Ala Ile Pro Gln Val Glu Phe Ile

405 410 415

Lys Asp Ser Pro Ala Leu Ile Val Ala Arg Val Leu Ser Ser Leu Ile

420 425 430

Asn Glu Ser Val Ile Met Val Glu Ser Gly Val Cys Ser Arg Glu Asp

435 440 445

Ile Asp Val Ala Ala Val Ala Gly Val Asn Tyr Ala Asp Gly Ile Phe

450 455 460

Gly Trp Leu Thr Arg Leu Gly Glu Glu Asn Val Arg Thr Thr Leu Ser

465 470 475 480

Asn Leu Ala Gln Leu Leu His Ala Ala Arg Tyr Ala Pro His Tyr Thr

485 490 495

Leu Leu His Ala Ala Gln Pro Ala Leu Thr Thr Thr Pro

500 505

<210> 67

<211> 509

<212> PRT

<213> Serratia nematodiphila WCU338

<400> 67

Met Ala Glu Ser Asn Ala Ala Ile Gln Ser Ala Ala Ile Ile Gly Ala

1 5 10 15

Gly Thr Met Gly Arg Gly Ile Ala Tyr Leu Phe Ala Gln Lys Ser Ile

20 25 30

Arg Thr Val Leu Tyr Asn Arg Asn Gly Asn Thr Leu Asn Gln Ala Arg

35 40 45

Asp Ala Ile Val Gln Asp Leu Asn Lys Lys Val Glu Gln Gly Lys Leu

50 55 60

Ala Leu Gln Asp Lys Asp Ala Val Leu Ala Asn Leu Thr Phe Ser Ala

65 70 75 80

Glu Phe Gly Ala Ile Ala Asp Ser Asp Leu Val Ile Glu Thr Ile Ala

85 90 95

Glu His Glu Gln Ala Lys Leu Glu Val Leu Ala Ala Ile Ala Ala Thr

100 105 110

Val Lys Pro Asp Thr Leu Ile Ala Thr Asn Thr Ser Ser Leu Ser Leu

115 120 125

Asn Lys Leu Ala Thr Ala Val Thr His Ser Glu Arg Phe Ile Gly Leu

130 135 140

His Phe Phe Asn Pro Ala Pro Leu Met Lys Leu Ile Glu Ile Ile Pro

145 150 155 160

Ala Tyr Phe Thr Ala Gln Ile Thr Thr Glu Arg Cys Arg Gln Leu Val

165 170 175

Ala Ala Leu Gly Lys Arg Asp Val Val Cys Gln Ala Thr Pro Gly Phe

180 185 190

Ile Val Asn Arg Met Ala Arg Pro Tyr Tyr Leu Glu Gly Phe Arg Leu

195 200 205

Leu Glu Glu His Val Ala Arg Ala Pro Gln Ile Asp Arg Ala Leu Lys

210 215 220

Ala Gly Gly His Phe Arg Met Gly Pro Leu Glu Leu Thr Asp Phe Ile

225 230 235 240

Gly Gln Asp Ile Asn Tyr Gln Val Ser Arg Gln Ile Trp Gln Asp Met

245 250 255

Gln Tyr Asp Pro Arg Tyr Thr Pro Gly His Leu Gln Arg Ser Leu Val

260 265 270

Asp Ala Gly Leu Leu Gly Lys Lys Asn Gly Arg Ser Tyr Phe Ser Ala

275 280 285

Glu Glu Ser Pro Pro Pro Leu Ala Ala Ala Val Asp Ala Glu Val Glu

290 295 300

Thr Leu Arg Ile Tyr Gly Glu His Pro Leu Phe Thr Leu Leu Gln Gln

305 310 315 320

Arg Ala Ala Leu Gln Trp Pro Arg Leu Arg Val Glu Gln Arg Pro Thr

325 330 335

Leu Pro Gly Leu Gly Ala Ala Ile Gln Val Asn Asp Ala Phe Thr Val

340 345 350

Ser Val Thr Asp Gly Arg Thr Ala Asn Gln Leu Ala Glu Gln Thr Ala

355 360 365

Ala Asp Ala Phe Val Val Asp Val Ala Leu Asn Tyr Gly Asp Thr Ala

370 375 380

Tyr Leu Val Ala Ala His Ser Arg His Ala Ser Ala Ala Asn Lys Ala

385 390 395 400

Leu Phe Leu Arg Leu Leu His Thr Ala Ile Pro Gln Val Glu Phe Ile

405 410 415

Lys Asp Ser Pro Ala Leu Ile Val Ala Arg Val Leu Ser Ser Leu Ile

420 425 430

Asn Glu Ser Val Ile Met Val Glu Ser Gly Val Cys Ser Arg Glu Asp

435 440 445

Ile Asp Val Ala Ala Val Ala Gly Val Asn Tyr Ala Asp Gly Ile Phe

450 455 460

Gly Trp Leu Thr Arg Leu Gly Glu Glu Asn Val Arg Thr Thr Leu Ser

465 470 475 480

Asn Leu Ala Gln Leu Leu His Ala Ala Arg Tyr Ala Pro His Tyr Thr

485 490 495

Leu Leu His Ala Ala Gln Pro Ala Leu Thr Thr Thr Pro

500 505

<210> 68

<211> 509

<212> PRT

<213> Serratia sp. OLEL1

<400> 68

Met Ala Glu Ser Asn Ala Ala Ile Gln Ser Ala Ala Ile Ile Gly Ala

1 5 10 15

Gly Thr Met Gly Arg Gly Ile Ala Tyr Leu Leu Ala Gln Lys Ser Ile

20 25 30

Arg Thr Val Leu Tyr Asn Arg Asn Gly Asn Thr Leu Asn Gln Ala Arg

35 40 45

Asp Ala Ile Val Gln Asp Leu Asn Lys Lys Val Glu Gln Gly Lys Leu

50 55 60

Ala Leu Gln Asp Lys Asp Ala Val Leu Ala Asn Leu Thr Phe Ser Ala

65 70 75 80

Glu Phe Gly Ala Ile Ala Asp Ser Asp Leu Val Ile Glu Thr Ile Ala

85 90 95

Glu His Glu Gln Ala Lys Leu Glu Val Leu Ala Ala Ile Ala Ala Thr

100 105 110

Val Lys Pro Asp Thr Leu Ile Ala Thr Asn Thr Ser Ser Leu Ser Leu

115 120 125

Asn Lys Leu Ala Thr Ala Val Thr His Ser Glu Arg Phe Ile Gly Leu

130 135 140

His Phe Phe Asn Pro Ala Pro Leu Met Lys Leu Ile Glu Ile Ile Pro

145 150 155 160

Ala Tyr Phe Thr Ala Gln Val Thr Thr Glu Arg Cys Arg Gln Leu Val

165 170 175

Ala Ala Leu Gly Lys Arg Asp Val Val Cys Gln Ala Thr Pro Gly Phe

180 185 190

Ile Val Asn Arg Met Ala Arg Pro Tyr Tyr Leu Glu Gly Phe Arg Leu

195 200 205

Leu Glu Glu His Val Ala Arg Ala Pro Gln Ile Asp Arg Ala Leu Lys

210 215 220

Ala Gly Gly His Phe Arg Met Gly Pro Leu Glu Leu Thr Asp Phe Ile

225 230 235 240

Gly Gln Asp Ile Asn Tyr Gln Val Ser Arg Gln Ile Trp Gln Asp Met

245 250 255

Gln Tyr Asp Pro Arg Tyr Thr Pro Gly His Leu Gln Arg Ser Leu Val

260 265 270

Asp Ala Gly Leu Leu Gly Lys Lys Asn Gly Arg Ser Tyr Phe Ser Ala

275 280 285

Glu Glu Ser Pro Pro Pro Leu Ala Ala Ala Val Asp Ala Glu Val Glu

290 295 300

Thr Leu Arg Ile Tyr Gly Glu His Pro Leu Phe Thr Leu Leu Gln Gln

305 310 315 320

Arg Ala Ala Leu Gln Trp Pro Arg Leu Arg Val Glu Gln Arg Pro Thr

325 330 335

Leu Pro Gly Leu Gly Ala Ala Ile Gln Val Asn Asp Ala Phe Thr Val

340 345 350

Ser Val Thr Asp Gly Arg Thr Ala Asn Gln Leu Ala Glu Gln Thr Ala

355 360 365

Ala Asp Ala Phe Val Val Asp Val Ala Leu Asn Tyr Gly Asp Thr Ala

370 375 380

Tyr Leu Val Ala Ala His Ser Arg His Ala Ser Ala Ala Asn Lys Ala

385 390 395 400

Leu Phe Leu Arg Leu Leu His Thr Ala Ile Pro Gln Val Glu Phe Ile

405 410 415

Lys Asp Ser Pro Ala Leu Ile Val Ala Arg Val Leu Ser Ser Leu Ile

420 425 430

Asn Glu Ser Val Ile Met Val Glu Ser Gly Val Cys Ser Arg Glu Asp

435 440 445

Ile Asp Val Ala Ala Val Ala Gly Val Asn Tyr Ala Asp Gly Ile Phe

450 455 460

Gly Trp Leu Thr Arg Leu Gly Glu Glu Asn Val Arg Thr Thr Leu Ser

465 470 475 480

Asn Leu Ala Gln Leu Leu His Ala Ala Arg Tyr Ala Pro His Tyr Thr

485 490 495

Leu Leu His Ala Ala Gln Pro Ala Leu Thr Thr Thr Pro

500 505

<210> 69

<211> 509

<212> PRT

<213> Serratia marcescens 7209

<400> 69

Met Ala Glu Ser Asn Ala Ala Ile Gln Ser Ala Ala Ile Ile Gly Ala

1 5 10 15

Gly Thr Met Gly Arg Gly Ile Ala Tyr Leu Phe Ala Gln Lys Ser Ile

20 25 30

Arg Thr Val Leu Tyr Asn Arg Asn Gly Asn Thr Leu Asn Gln Ala Arg

35 40 45

Asp Ala Ile Val Gln Asp Leu Asn Lys Lys Val Glu Gln Gly Lys Leu

50 55 60

Ala Leu Gln Asp Lys Asp Ala Val Leu Ala Asn Leu Thr Phe Ser Ala

65 70 75 80

Glu Phe Gly Val Ile Ala Asp Ser Asp Leu Val Ile Glu Thr Ile Ala

85 90 95

Glu His Glu Gln Ala Lys Leu Glu Val Leu Ala Ala Ile Ala Ala Thr

100 105 110

Val Lys Pro Asp Thr Leu Ile Ala Thr Asn Thr Ser Ser Leu Ser Leu

115 120 125

Asn Lys Leu Ala Thr Ala Val Thr His Ser Glu Arg Phe Ile Gly Leu

130 135 140

His Phe Phe Asn Pro Ala Pro Leu Met Lys Leu Ile Glu Ile Ile Pro

145 150 155 160

Ala Tyr Phe Thr Ala Gln Val Thr Thr Glu Arg Cys Arg Gln Leu Val

165 170 175

Ala Ala Leu Gly Lys Arg Asp Val Val Cys Gln Ala Thr Pro Gly Phe

180 185 190

Ile Val Asn Arg Met Ala Arg Pro Tyr Tyr Leu Glu Gly Phe Arg Leu

195 200 205

Leu Glu Glu His Val Ala Arg Ala Pro Gln Ile Asp Arg Ala Leu Lys

210 215 220

Ala Gly Gly His Phe Arg Met Gly Pro Leu Glu Leu Thr Asp Phe Ile

225 230 235 240

Gly Gln Asp Ile Asn Tyr Gln Val Ser Arg Gln Ile Trp Gln Asp Met

245 250 255

Gln Tyr Asp Pro Arg Tyr Thr Pro Gly His Leu Gln Arg Ser Leu Val

260 265 270

Asp Ala Gly Leu Leu Gly Lys Lys Asn Gly Arg Ser Tyr Phe Ser Ala

275 280 285

Glu Glu Ser Pro Pro Pro Leu Ala Ala Ala Val Asp Ala Glu Val Glu

290 295 300

Thr Leu Arg Ile Tyr Gly Glu His Pro Leu Phe Thr Leu Leu Gln Gln

305 310 315 320

Arg Ala Ala Leu Gln Trp Pro Arg Leu Arg Val Glu Gln Arg Pro Thr

325 330 335

Leu Pro Gly Leu Gly Ala Ala Ile Gln Val Asn Asp Ala Phe Thr Val

340 345 350

Ser Val Thr Asp Gly Arg Thr Ala Asn Gln Leu Ala Glu Gln Thr Ala

355 360 365

Ala Asp Ala Phe Val Val Asp Val Ala Leu Asn Tyr Gly Asp Thr Ala

370 375 380

Tyr Leu Val Ala Ala His Ser Arg His Ala Ser Ala Ala Asn Lys Ala

385 390 395 400

Leu Phe Leu Arg Leu Leu His Thr Ala Ile Pro Gln Val Glu Phe Ile

405 410 415

Lys Asp Ser Pro Ala Leu Ile Val Ala Arg Val Leu Ser Ser Leu Ile

420 425 430

Asn Glu Ser Val Ile Met Val Glu Ser Gly Val Cys Ser Arg Glu Asp

435 440 445

Ile Asp Val Ala Ala Val Ala Gly Val Asn Tyr Ala Asp Gly Ile Phe

450 455 460

Gly Trp Leu Thr Arg Leu Gly Glu Glu Asn Val Arg Thr Thr Leu Ser

465 470 475 480

Asn Leu Ala Gln Leu Leu His Ala Ala Arg Tyr Ala Pro His Tyr Thr

485 490 495

Leu Leu His Ala Ala Gln Pro Ala Leu Thr Thr Thr Pro

500 505

<210> 70

<211> 509

<212> PRT

<213> Serratia marcescens sicaria (Ss1)

<400> 70

Met Ala Glu Ser Asn Ala Ala Ile Gln Ser Ala Ala Ile Ile Gly Ala

1 5 10 15

Gly Thr Met Gly Arg Gly Ile Ala Tyr Leu Phe Ala Gln Lys Ser Ile

20 25 30

Arg Thr Val Leu Tyr Asn Arg Asn Gly Asn Thr Leu Asn Gln Ala Arg

35 40 45

Asp Ala Ile Val Gln Asp Leu Asn Lys Lys Val Glu Gln Gly Lys Leu

50 55 60

Ala Leu Gln Asp Lys Asp Ala Val Leu Ala Asn Leu Thr Phe Ser Ala

65 70 75 80

Glu Phe Gly Ala Ile Ala Asp Ser Asp Leu Val Ile Glu Thr Ile Ala

85 90 95

Glu His Glu Gln Ala Lys Leu Glu Val Leu Ala Ala Ile Ala Ala Thr

100 105 110

Val Lys Pro Asp Thr Leu Ile Ala Thr Asn Thr Ser Ser Leu Ser Leu

115 120 125

Asn Lys Leu Ala Thr Ala Val Thr His Ser Glu Arg Phe Ile Gly Leu

130 135 140

His Phe Phe Asn Pro Ala Pro Leu Met Lys Leu Ile Glu Ile Ile Pro

145 150 155 160

Ala Tyr Phe Thr Ala Gln Val Thr Thr Glu Arg Cys Arg Gln Leu Val

165 170 175

Ala Ala Leu Gly Lys Arg Asp Val Val Cys Gln Ala Thr Pro Gly Phe

180 185 190

Ile Val Asn Arg Met Ala Arg Pro Tyr Tyr Leu Glu Gly Phe Arg Leu

195 200 205

Leu Glu Glu His Val Ala Arg Ala Pro Gln Ile Asp Arg Ala Leu Lys

210 215 220

Ala Gly Gly His Phe Arg Met Gly Pro Leu Glu Leu Thr Asp Phe Ile

225 230 235 240

Gly Gln Asp Ile Asn Tyr Gln Val Ser Arg Gln Ile Trp Gln Asp Met

245 250 255

Gln Tyr Asp Pro Arg Tyr Thr Pro Gly His Leu Gln Arg Ser Leu Val

260 265 270

Asp Ala Gly Leu Leu Gly Lys Lys Asn Gly Arg Ser Tyr Phe Ser Thr

275 280 285

Glu Glu Ser Pro Pro Pro Leu Ala Ala Ala Val Asp Ala Glu Val Glu

290 295 300

Thr Leu Arg Ile Tyr Gly Glu His Pro Leu Phe Thr Leu Leu Gln Gln

305 310 315 320

Arg Ala Ala Leu Gln Trp Pro Arg Leu Arg Val Glu Gln Arg Pro Thr

325 330 335

Leu Pro Gly Leu Gly Ala Ala Ile Gln Val Asn Asp Ala Phe Thr Val

340 345 350

Ser Val Thr Asp Gly Arg Thr Ala Asn Gln Leu Ala Glu Gln Thr Ala

355 360 365

Ala Asp Ala Phe Val Val Asp Val Ala Leu Asn Tyr Gly Asp Thr Ala

370 375 380

Tyr Leu Val Ala Ala His Ser Arg His Ala Ser Ala Ala Asn Lys Ala

385 390 395 400

Leu Phe Leu Arg Leu Leu His Thr Ala Ile Pro Gln Val Glu Phe Ile

405 410 415

Lys Asp Ser Pro Ala Leu Ile Val Ala Arg Val Leu Ser Ser Leu Ile

420 425 430

Asn Glu Ser Val Ile Met Val Glu Ser Gly Val Cys Ser Arg Glu Asp

435 440 445

Ile Asp Val Ala Ala Val Ala Gly Val Asn Tyr Ala Asp Gly Ile Phe

450 455 460

Gly Trp Leu Thr Arg Leu Gly Glu Glu Asn Val Arg Thr Thr Leu Ser

465 470 475 480

Asn Leu Ala Gln Leu Leu His Ala Ala Arg Tyr Ala Pro His Tyr Thr

485 490 495

Leu Leu His Ala Ala Gln Pro Ala Leu Thr Thr Thr Pro

500 505

<210> 71

<211> 509

<212> PRT

<213> Serratia sp. OLFL2

<400> 71

Met Ala Glu Ser Asn Ala Ala Ile Gln Ser Ala Ala Ile Ile Gly Ala

1 5 10 15

Gly Thr Met Gly Arg Gly Ile Ala Tyr Leu Leu Ala Gln Lys Ser Ile

20 25 30

Arg Thr Val Leu Tyr Asn Arg Asn Gly Asn Thr Leu Asn Gln Ala Arg

35 40 45

Asp Ala Ile Val Gln Asp Leu Asn Lys Lys Val Glu Gln Gly Lys Leu

50 55 60

Ala Leu Gln Asp Lys Asp Ala Val Leu Ala Asn Leu Thr Phe Ser Ala

65 70 75 80

Glu Phe Gly Ala Ile Ala Asp Ser Asp Leu Val Ile Glu Thr Ile Ala

85 90 95

Glu His Glu Gln Ala Lys Leu Asp Val Leu Ala Ala Ile Ala Ala Thr

100 105 110

Val Lys Pro Asp Thr Leu Ile Ala Thr Asn Thr Ser Ser Leu Ser Leu

115 120 125

Asn Lys Leu Ala Thr Ala Val Thr His Ser Glu Arg Phe Ile Gly Leu

130 135 140

His Phe Phe Asn Pro Ala Pro Leu Met Lys Leu Ile Glu Ile Ile Pro

145 150 155 160

Ala Tyr Phe Thr Ala Gln Val Thr Thr Glu Arg Cys Arg Gln Leu Val

165 170 175

Ala Ala Leu Gly Lys Arg Asp Val Val Cys Gln Ala Thr Pro Gly Phe

180 185 190

Ile Val Asn Arg Met Ala Arg Pro Tyr Tyr Leu Glu Gly Phe Arg Leu

195 200 205

Leu Glu Glu His Val Ala Arg Ala Pro Gln Ile Asp Arg Ala Leu Lys

210 215 220

Ala Gly Gly His Phe Arg Met Gly Pro Leu Glu Leu Thr Asp Phe Ile

225 230 235 240

Gly Gln Asp Ile Asn Tyr Gln Val Ser Arg Gln Ile Trp Gln Asp Met

245 250 255

Gln Tyr Asp Pro Arg Tyr Thr Pro Gly His Leu Gln Arg Ser Leu Val

260 265 270

Asp Ala Gly Leu Leu Gly Lys Lys Asn Gly Arg Ser Tyr Phe Ser Ala

275 280 285

Glu Glu Ser Pro Pro Pro Leu Ala Ala Ala Val Asp Ala Glu Val Glu

290 295 300

Thr Leu Arg Ile Tyr Gly Glu His Pro Leu Phe Thr Leu Leu Gln Gln

305 310 315 320

Arg Ala Ala Leu Gln Trp Pro Arg Leu Arg Val Glu Gln Arg Pro Thr

325 330 335

Leu Pro Gly Leu Gly Ala Ala Ile Gln Val Asn Asp Ala Phe Thr Val

340 345 350

Ser Val Thr Asp Gly Arg Thr Ala Asn Gln Leu Ala Glu Gln Thr Ala

355 360 365

Ala Asp Ala Phe Val Val Asp Val Ala Leu Asn Tyr Gly Asp Thr Ala

370 375 380

Tyr Leu Val Ala Ala His Ser Arg His Ala Ser Ala Ala Asn Lys Ala

385 390 395 400

Leu Phe Leu Arg Leu Leu His Thr Ala Ile Pro Gln Val Glu Phe Ile

405 410 415

Lys Asp Ser Pro Ala Leu Ile Val Ala Arg Val Leu Ser Ser Leu Ile

420 425 430

Asn Glu Ser Val Ile Met Val Glu Ser Gly Val Cys Ser Arg Glu Asp

435 440 445

Ile Asp Val Ala Ala Val Ala Gly Val Asn Tyr Ala Asp Gly Ile Phe

450 455 460

Gly Trp Leu Thr Arg Leu Gly Glu Glu Asn Val Arg Thr Thr Leu Ser

465 470 475 480

Asn Leu Ala Gln Leu Leu His Ala Ala Arg Tyr Ala Pro His Tyr Thr

485 490 495

Leu Leu His Ala Ala Gln Pro Ala Leu Thr Thr Thr Pro

500 505

<210> 72

<211> 509

<212> PRT

<213> Serratia marcescens BIDMC 81

<400> 72

Met Ala Glu Ser Asn Ala Ala Ile Gln Ser Ala Ala Ile Ile Gly Ala

1 5 10 15

Gly Thr Met Gly Arg Gly Ile Ala Tyr Leu Leu Ala Gln Lys Ser Ile

20 25 30

Arg Thr Val Leu Tyr Asn Arg Asn Gly Asn Thr Leu Asn Gln Ala Arg

35 40 45

Asp Ala Ile Val Gln Asp Leu Asn Lys Lys Val Glu Gln Gly Lys Leu

50 55 60

Ala Leu Gln Asp Lys Asp Ala Val Leu Ala Asn Leu Thr Phe Ser Ala

65 70 75 80

Glu Phe Gly Ala Ile Ala Asp Ser Asp Leu Val Ile Glu Thr Ile Ala

85 90 95

Glu His Glu Gln Ala Lys Leu Glu Val Leu Ala Ala Ile Ala Ala Thr

100 105 110

Val Lys Pro Asp Thr Leu Ile Ala Thr Asn Thr Ser Ser Leu Ser Leu

115 120 125

Asn Lys Leu Ala Thr Ala Val Thr His Ser Glu Arg Phe Ile Gly Leu

130 135 140

His Phe Phe Asn Pro Ala Pro Leu Met Lys Leu Ile Glu Ile Ile Pro

145 150 155 160

Ala Tyr Phe Thr Ala Gln Val Thr Thr Glu Arg Cys Arg Gln Leu Val

165 170 175

Ala Ala Leu Gly Lys Arg Asp Val Val Cys Gln Ala Thr Pro Gly Phe

180 185 190

Ile Val Asn Arg Met Ala Arg Pro Tyr Tyr Leu Glu Gly Phe Arg Leu

195 200 205

Leu Glu Glu His Val Ala Arg Ala Pro Gln Ile Asp Arg Ala Leu Lys

210 215 220

Ala Gly Gly His Phe Arg Met Gly Pro Leu Glu Leu Thr Asp Phe Ile

225 230 235 240

Gly Gln Asp Ile Asn Tyr Gln Val Ser Arg Gln Ile Trp Gln Asp Met

245 250 255

Gln Tyr Asp Pro Arg Tyr Thr Pro Gly His Leu Gln Arg Ser Leu Val

260 265 270

Asp Ala Gly Leu Leu Gly Lys Lys Asn Gly Arg Ser Tyr Phe Ser Ala

275 280 285

Glu Glu Ser Pro Pro Pro Leu Ala Ala Ala Val Asp Ala Glu Val Glu

290 295 300

Thr Leu Arg Ile Tyr Gly Glu His Pro Leu Phe Thr Leu Leu Gln Gln

305 310 315 320

Arg Ala Ala Leu Gln Trp Pro Arg Leu Arg Val Glu Gln Arg Pro Thr

325 330 335

Leu Pro Asp Leu Gly Ala Ala Ile Gln Val Asn Asp Ala Phe Thr Val

340 345 350

Ser Val Thr Asp Gly Arg Thr Ala Asn Gln Leu Ala Glu Gln Thr Ala

355 360 365

Ala Asp Ala Phe Val Val Asp Val Ala Leu Asn Tyr Gly Asp Thr Ala

370 375 380

Tyr Leu Val Ala Ala His Ser Arg His Ala Ser Ala Ala Asn Lys Ala

385 390 395 400

Leu Phe Leu Arg Leu Leu His Thr Ala Ile Pro Gln Val Glu Phe Ile

405 410 415

Lys Asp Ser Pro Ala Leu Ile Val Ala Arg Val Leu Ser Ser Leu Ile

420 425 430

Asn Glu Ser Val Ile Met Val Glu Ser Gly Val Cys Ser Arg Glu Asp

435 440 445

Ile Asp Val Ala Ala Val Ala Gly Val Asn Tyr Ala Asp Gly Ile Phe

450 455 460

Gly Trp Leu Thr Arg Leu Gly Glu Glu Asn Val Arg Thr Thr Leu Ser

465 470 475 480

Asn Leu Ala Gln Leu Leu His Ala Ala Arg Tyr Ala Pro His Tyr Thr

485 490 495

Leu Leu His Ala Ala Gln Pro Ala Leu Thr Thr Thr Pro

500 505

<210> 73

<211> 509

<212> PRT

<213> Serratia marcescens BIDMC 50

<400> 73

Met Ala Glu Ser Asn Ala Ala Ile Gln Ser Ala Ala Ile Ile Gly Ala

1 5 10 15

Gly Thr Met Gly Arg Gly Ile Ala Tyr Leu Phe Ala Gln Lys Ser Ile

20 25 30

Arg Thr Val Leu Tyr Asn Arg Asn Gly Asn Thr Leu Asn Gln Ala Arg

35 40 45

Asp Ala Ile Val Gln Asp Leu Asn Lys Lys Val Glu Gln Gly Lys Leu

50 55 60

Ala Leu Gln Asp Lys Asp Val Val Leu Ala Asn Leu Thr Phe Ser Ala

65 70 75 80

Glu Phe Gly Ala Ile Ala Asp Ser Asp Leu Val Ile Glu Thr Ile Ala

85 90 95

Glu His Glu Gln Ala Lys Leu Glu Val Leu Ala Ala Ile Ala Ala Thr

100 105 110

Val Lys Pro Asp Thr Leu Ile Ala Thr Asn Thr Ser Ser Leu Ser Leu

115 120 125

Asn Lys Leu Ala Thr Ala Val Thr His Ser Glu Arg Phe Ile Gly Leu

130 135 140

His Phe Phe Asn Pro Ala Pro Leu Met Lys Leu Ile Glu Ile Ile Pro

145 150 155 160

Ala Tyr Phe Thr Ala Gln Val Thr Thr Glu Arg Cys Arg Gln Leu Val

165 170 175

Ala Ala Leu Gly Lys Arg Asp Val Val Cys Gln Ala Thr Pro Gly Phe

180 185 190

Ile Val Asn Arg Met Ala Arg Pro Tyr Tyr Leu Glu Gly Phe Arg Leu

195 200 205

Leu Glu Glu His Val Ala Arg Ala Pro Gln Ile Asp Arg Ala Leu Lys

210 215 220

Ala Gly Gly His Phe Arg Met Gly Pro Leu Glu Leu Thr Asp Phe Ile

225 230 235 240

Gly Gln Asp Ile Asn Tyr Gln Val Ser Arg Gln Ile Trp Gln Asp Met

245 250 255

Gln Tyr Asp Pro Arg Tyr Thr Pro Gly His Leu Gln Arg Ser Leu Val

260 265 270

Asp Ala Gly Leu Leu Gly Lys Lys Asn Gly Arg Ser Tyr Phe Ser Ala

275 280 285

Glu Glu Ser Pro Pro Pro Leu Ala Ala Ala Val Asp Ala Glu Val Glu

290 295 300

Thr Leu Arg Ile Tyr Gly Glu His Pro Leu Phe Thr Leu Leu Gln Gln

305 310 315 320

Arg Ala Ala Leu Gln Trp Pro Arg Leu Arg Val Glu Gln Arg Pro Thr

325 330 335

Leu Pro Asp Leu Gly Ala Ala Ile Gln Val Asn Asp Ala Phe Thr Val

340 345 350

Ser Val Thr Asp Gly Arg Thr Ala Asn Gln Leu Ala Glu Gln Thr Ala

355 360 365

Ala Asp Ala Phe Val Val Asp Val Ala Leu Asn Tyr Gly Asp Thr Ala

370 375 380

Tyr Leu Val Ala Ala His Ser Arg His Ala Ser Ala Ala Asn Lys Ala

385 390 395 400

Leu Phe Leu Arg Leu Leu His Thr Ala Ile Pro Gln Val Glu Phe Ile

405 410 415

Lys Asp Ser Pro Ala Leu Ile Val Ala Arg Val Leu Ser Ser Leu Ile

420 425 430

Asn Glu Ser Val Ile Met Val Glu Ser Gly Val Cys Ser Arg Glu Asp

435 440 445

Ile Asp Val Ala Ala Val Ala Gly Val Asn Tyr Ala Asp Gly Ile Phe

450 455 460

Gly Trp Leu Thr Arg Leu Gly Glu Glu Asn Val Arg Thr Thr Leu Ser

465 470 475 480

Asn Leu Ala Gln Leu Leu His Ala Ala Arg Tyr Ala Pro His Tyr Thr

485 490 495

Leu Leu His Ala Ala Gln Pro Ala Leu Thr Thr Thr Pro

500 505

<210> 74

<211> 509

<212> PRT

<213> Serratia marcescens UMH7

<400> 74

Met Ala Glu Ser Asn Ala Ala Ile Gln Ser Ala Ala Ile Ile Gly Ala

1 5 10 15

Gly Thr Met Gly Arg Gly Ile Ala Tyr Leu Leu Ala Gln Lys Ser Ile

20 25 30

Arg Thr Val Leu Tyr Asn Arg Asn Gly Asn Thr Leu Asn Gln Ala Arg

35 40 45

Asp Ala Ile Val Gln Asp Leu Asn Lys Lys Val Glu Gln Gly Lys Leu

50 55 60

Ala Leu Gln Asp Lys Asp Thr Val Leu Ala Asn Leu Thr Phe Ser Ala

65 70 75 80

Glu Phe Gly Ala Ile Ala Asp Ser Asp Leu Val Ile Glu Thr Ile Ala

85 90 95

Glu His Glu Gln Ala Lys Leu Glu Val Leu Ala Ala Ile Ala Ala Thr

100 105 110

Val Lys Pro Asp Thr Leu Ile Ala Thr Asn Thr Ser Ser Leu Ser Leu

115 120 125

Asn Lys Leu Ala Thr Ala Val Thr His Ser Glu Arg Phe Ile Gly Leu

130 135 140

His Phe Phe Asn Pro Ala Pro Leu Met Lys Leu Ile Glu Ile Ile Pro

145 150 155 160

Ala Tyr Phe Thr Ala Gln Val Thr Thr Glu Arg Cys Arg Gln Leu Val

165 170 175

Ala Ala Leu Gly Lys Arg Asp Val Val Cys Gln Ala Thr Pro Gly Phe

180 185 190

Ile Val Asn Arg Met Ala Arg Pro Tyr Tyr Leu Glu Gly Phe Arg Leu

195 200 205

Leu Glu Glu His Val Ala Arg Ala Pro Gln Ile Asp Arg Ala Leu Lys

210 215 220

Ala Gly Gly His Phe Arg Met Gly Pro Leu Glu Leu Thr Asp Phe Ile

225 230 235 240

Gly Gln Asp Ile Asn Tyr Gln Val Ser Arg Gln Ile Trp Gln Asp Met

245 250 255

Gln Tyr Asp Pro Arg Tyr Thr Pro Gly His Leu Gln Arg Ser Leu Val

260 265 270

Asp Ala Gly Leu Leu Gly Lys Lys Asn Gly Arg Ser Tyr Phe Ser Ala

275 280 285

Glu Glu Ser Pro Pro Pro Leu Ala Ala Ala Val Asp Ala Glu Val Glu

290 295 300

Thr Leu Arg Ile Tyr Gly Glu His Pro Leu Phe Thr Leu Leu Gln Gln

305 310 315 320

Arg Ala Thr Leu Gln Trp Pro Arg Leu Arg Val Glu Gln Arg Pro Thr

325 330 335

Leu Pro Gly Leu Gly Ala Ala Ile Gln Val Asn Asp Ala Phe Thr Val

340 345 350

Ser Val Thr Asp Gly Arg Thr Ala Asn Gln Leu Ala Glu Gln Thr Ala

355 360 365

Ala Asp Ala Phe Val Val Asp Val Ala Leu Asn Tyr Gly Asp Thr Ala

370 375 380

Tyr Leu Val Ala Ala His Ser Arg His Ala Ser Ala Ala Asn Lys Ala

385 390 395 400

Leu Phe Leu Arg Leu Leu His Thr Ala Ile Pro Gln Val Glu Phe Ile

405 410 415

Lys Asp Ser Pro Ala Leu Ile Val Ala Arg Val Leu Ser Ser Leu Ile

420 425 430

Asn Glu Ser Val Ile Met Val Glu Ser Gly Val Cys Ser Arg Glu Asp

435 440 445

Ile Asp Val Ala Ala Val Ala Gly Val Asn Tyr Ala Asp Gly Ile Phe

450 455 460

Gly Trp Leu Thr Arg Leu Gly Glu Glu Asn Val Arg Thr Thr Leu Ser

465 470 475 480

Asn Leu Ala Gln Leu Leu His Ala Ala Arg Tyr Ala Pro His Tyr Thr

485 490 495

Leu Leu His Ala Ala Gln Pro Ala Leu Thr Thr Thr Pro

500 505

<210> 75

<211> 509

<212> PRT

<213> Serratia marcescens RSC-14

<400> 75

Met Ala Glu Ser Asn Ala Ala Ile Gln Ser Ala Ala Ile Ile Gly Ala

1 5 10 15

Gly Thr Met Gly Arg Gly Ile Ala Tyr Leu Leu Ala Gln Lys Ser Ile

20 25 30

Arg Thr Val Leu Tyr Asn Arg Asn Gly Asn Thr Leu Asn Gln Ala Arg

35 40 45

Asp Ala Ile Val Gln Asp Leu Asn Lys Lys Val Glu Gln Gly Lys Leu

50 55 60

Ala Leu Gln Asp Lys Asp Ala Val Leu Ala Asn Leu Thr Phe Ser Thr

65 70 75 80

Glu Phe Gly Ala Ile Ala Asp Ser Asp Leu Val Ile Glu Thr Ile Ala

85 90 95

Glu His Glu Gln Ala Lys Leu Glu Val Leu Ala Ala Ile Ala Ala Thr

100 105 110

Val Lys Pro Asp Arg Leu Ile Ala Thr Asn Thr Ser Ser Leu Ser Leu

115 120 125

Asn Lys Leu Ala Thr Ala Val Thr His Ser Glu Arg Phe Ile Gly Leu

130 135 140

His Phe Phe Asn Pro Ala Pro Leu Met Lys Leu Ile Glu Ile Ile Pro

145 150 155 160

Ala Tyr Phe Thr Ala Gln Val Thr Thr Glu Arg Cys Arg Gln Leu Val

165 170 175

Ala Ala Leu Gly Lys Arg Asp Val Val Cys Gln Ala Thr Pro Gly Phe

180 185 190

Ile Val Asn Arg Met Ala Arg Pro Tyr Tyr Leu Glu Gly Phe Arg Leu

195 200 205

Leu Glu Glu His Val Ala Arg Ala Pro Gln Ile Asp Arg Ala Leu Lys

210 215 220

Ala Gly Gly His Phe Arg Met Gly Pro Leu Glu Leu Thr Asp Phe Ile

225 230 235 240

Gly Gln Asp Ile Asn Tyr Gln Val Ser Arg Gln Ile Trp Gln Asp Met

245 250 255

Gln Tyr Asp Pro Arg Tyr Thr Pro Gly His Leu Gln Arg Ser Leu Val

260 265 270

Asp Ala Gly Leu Leu Gly Lys Lys Asn Gly Arg Ser Tyr Phe Ser Ala

275 280 285

Glu Glu Ser Pro Pro Pro Leu Ala Ala Ala Val Asp Ala Glu Val Glu

290 295 300

Thr Leu Arg Ile Tyr Gly Glu His Pro Leu Phe Thr Leu Leu Gln Gln

305 310 315 320

Arg Ala Ala Leu Gln Trp Pro Arg Leu Arg Val Glu Gln Gln Pro Thr

325 330 335

Leu Pro Gly Leu Gly Ala Ala Ile Gln Val Asn Asp Ala Phe Thr Val

340 345 350

Ser Val Thr Asp Gly Arg Thr Ala Asn Gln Leu Ala Glu Gln Thr Ala

355 360 365

Ala Asp Ala Phe Val Val Asp Val Ala Leu Asn Tyr Gly Asp Thr Ala

370 375 380

Tyr Leu Val Ala Ala His Ser Arg His Ala Ser Ala Ala Asn Lys Ala

385 390 395 400

Leu Phe Leu Arg Leu Leu His Thr Ala Ile Pro Gln Val Glu Phe Ile

405 410 415

Lys Asp Ser Pro Ala Leu Ile Val Ala Arg Val Leu Ser Ser Leu Ile

420 425 430

Asn Glu Ser Val Ile Met Val Glu Ser Gly Val Cys Ser Arg Glu Asp

435 440 445

Ile Asp Val Ala Ala Val Ala Gly Val Asn Tyr Ala Asp Gly Ile Phe

450 455 460

Gly Trp Leu Thr Arg Leu Gly Glu Glu Asn Val Arg Thr Thr Leu Ser

465 470 475 480

Asn Leu Ala Gln Leu Leu His Ala Ala Arg Tyr Ala Pro His Tyr Thr

485 490 495

Leu Leu His Ala Ala Gln Pro Ala Leu Thr Thr Thr Pro

500 505

<210> 76

<211> 509

<212> PRT

<213> Serratia marcescens SM03

<400> 76

Met Ala Glu Arg Asn Ala Ala Ile Gln Ser Ala Ala Ile Ile Gly Ala

1 5 10 15

Gly Thr Met Gly Arg Gly Ile Ala Tyr Leu Phe Ala Gln Lys Gly Ile

20 25 30

Arg Thr Val Leu Tyr Asn Arg Asn Gly Asn Thr Leu Ile Gln Ala Arg

35 40 45

Glu Tyr Ile Val Gln Asp Leu Asp Lys Lys Val Glu Gln Gly Arg Leu

50 55 60

Ala Pro Gln Asp Lys Asp Ala Val Leu Ala Asn Leu Gln Phe Ser Ser

65 70 75 80

Val Phe Glu Ala Ile Val Asp Ser Asp Leu Val Leu Glu Thr Ile Ala

85 90 95

Glu Gln Glu Gln Ala Lys Leu Glu Val Leu Ala Ala Ile Ala Ala Ala

100 105 110

Val Lys Pro Asp Thr Leu Ile Ala Thr Asn Thr Ser Ser Leu Ser Leu

115 120 125

Asn Lys Leu Ala Thr Ala Val Thr His Ser Glu Arg Phe Ile Gly Leu

130 135 140

His Phe Phe Asn Pro Ala Pro Leu Met Lys Leu Ile Glu Ile Ile Pro

145 150 155 160

Ala Tyr Phe Thr Ala Gln Ala Thr Thr Glu Thr Cys Arg Gln Leu Val

165 170 175

Ala Ala Leu Gly Lys Arg Asp Val Val Cys Gln Ala Thr Pro Gly Phe

180 185 190

Ile Val Asn Arg Met Ala Arg Pro Tyr Tyr Leu Glu Gly Phe Arg Leu

195 200 205

Leu Glu Glu His Val Ala Arg Ala Pro Gln Ile Asp Arg Ala Leu Lys

210 215 220

Ala Gly Gly His Phe Arg Met Gly Pro Leu Glu Leu Thr Asp Phe Ile

225 230 235 240

Gly Gln Asp Ile Asn Tyr Gln Val Ser Arg Gln Ile Trp Gln Asp Met

245 250 255

Gln Tyr Asp Pro Arg Tyr Thr Pro Gly His Leu Gln Arg Ser Leu Val

260 265 270

Asp Ala Gly Leu Leu Gly Lys Lys Asn Gly Arg Ser Tyr Phe Ser Ala

275 280 285

Glu Glu Thr Ala Pro Pro Val Glu Ala Ala Ala Glu Ala Asp Val Glu

290 295 300

Thr Leu Arg Ile Tyr Gly Glu His Pro Leu Phe Thr Leu Leu Gln Gln

305 310 315 320

Arg Ala Ala Leu Gln Trp Pro Gln Leu Arg Val Glu Gln Arg Pro Ala

325 330 335

Leu Ser Gly Leu Gly Ala Ala Ile Gln Val Asn Asp Ala Phe Thr Val

340 345 350

Ser Val Thr Asp Gly Arg Thr Ala Asn Gln Leu Ala Glu Gln Thr Ala

355 360 365

Ala Asp Ala Phe Val Val Asp Val Ala Leu Asn Tyr Ala Asp Thr Ala

370 375 380

Tyr Leu Val Ala Ala His Ser Arg His Ala Ser Ala Ala Asn Lys Ala

385 390 395 400

Leu Phe Leu Arg Leu Leu His Thr Ala Leu Pro Gln Val Glu Phe Ile

405 410 415

Lys Asp Ser Pro Ala Leu Ile Val Ala Arg Val Leu Ser Ser Leu Ile

420 425 430

Asn Glu Ser Val Ile Met Val Glu Ser Gly Val Cys Ser Arg Glu Asp

435 440 445

Ile Asp Val Ala Ala Val Ala Gly Val Asn Tyr Ala Asp Gly Ile Phe

450 455 460

Gly Trp Leu Thr Arg Leu Gly Glu Lys Asn Val Arg Thr Thr Leu Ser

465 470 475 480

Asn Leu Ala Gln Leu Leu His Ala Ala Arg Tyr Ala Pro His Tyr Thr

485 490 495

Leu Leu His Ala Ala Gln Pro Ala Leu Thr Thr Thr Pro

500 505

<210> 77

<211> 509

<212> PRT

<213> Serratia marcescens 90-166

<400> 77

Met Ala Glu Ser Asn Ala Ala Ile Gln Ser Ala Ala Ile Ile Gly Ala

1 5 10 15

Gly Thr Met Gly Arg Gly Ile Ala Tyr Leu Phe Ala Gln Lys Gly Ile

20 25 30

Arg Thr Val Leu Tyr Asn Arg Asn Gly Asn Thr Leu Ile Gln Ala Arg

35 40 45

Glu Tyr Ile Val Gln Asp Leu Asp Lys Lys Ile Glu Gln Gly Arg Val

50 55 60

Ala Pro Gln Asp Lys Asp Ala Val Leu Ala Asn Leu Thr Phe Ser Ala

65 70 75 80

Glu Phe Gly Ala Ile Val Asp Ser Asp Leu Val Ile Glu Thr Ile Ala

85 90 95

Glu His Glu Gln Ala Lys Leu Glu Val Leu Ala Ala Ile Ala Ala Thr

100 105 110

Val Lys Pro Asp Thr Leu Ile Ala Thr Asn Thr Ser Ser Leu Ser Leu

115 120 125

Asn Lys Leu Ala Thr Ala Val Thr His Ser Glu Arg Phe Ile Gly Leu

130 135 140

His Phe Phe Asn Pro Ala Pro Leu Met Lys Leu Ile Glu Ile Ile Pro

145 150 155 160

Ala Tyr Phe Thr Ala Gln Val Thr Thr Glu Arg Cys Arg Gln Leu Val

165 170 175

Ala Ala Leu Gly Lys Arg Asp Val Val Cys Gln Ala Thr Pro Gly Phe

180 185 190

Ile Val Asn Arg Met Ala Arg Pro Tyr Tyr Leu Glu Gly Phe Arg Leu

195 200 205

Leu Glu Glu His Val Ala Arg Ala Pro Gln Ile Asp Arg Ala Leu Lys

210 215 220

Ala Gly Gly His Phe Arg Met Gly Pro Leu Glu Leu Thr Asp Phe Ile

225 230 235 240

Gly Gln Asp Ile Asn Tyr Gln Val Ser Arg Gln Ile Trp Gln Asp Met

245 250 255

Gln Tyr Asp Pro Arg Tyr Thr Pro Gly His Leu Gln Arg Ser Leu Val

260 265 270

Asp Ala Gly Leu Leu Gly Lys Lys Asn Gly Arg Ser Tyr Phe Ser Ala

275 280 285

Glu Glu Ser Pro Pro Pro Leu Ala Ala Ala Val Asp Ala Glu Ile Glu

290 295 300

Thr Leu Arg Ile Tyr Gly Glu His Pro Leu Phe Thr Leu Leu Gln Gln

305 310 315 320

Arg Ala Ala Leu Gln Trp Pro Arg Leu Arg Val Glu His Arg Pro Thr

325 330 335

Leu Pro Gly Leu Gly Ala Ala Ile Gln Val Asn Asp Ala Phe Thr Val

340 345 350

Ser Val Thr Asp Gly Arg Thr Ala Asn Gln Leu Ala Glu Gln Thr Ala

355 360 365

Ala Asp Ala Phe Val Val Asp Val Ala Leu Asn Tyr Gly Asp Thr Ala

370 375 380

Tyr Leu Ala Ala Ala His Ser Arg His Ala Ser Ala Ala Asn Lys Ala

385 390 395 400

Leu Phe Leu Arg Leu Leu His Thr Ala Ile Pro Gln Val Glu Phe Ile

405 410 415

Lys Asp Ser Pro Ala Leu Ile Val Ala Arg Val Leu Ser Ser Leu Ile

420 425 430

Asn Glu Ser Val Ile Met Val Glu Ser Gly Val Cys Ser Arg Glu Asp

435 440 445

Ile Asp Val Ala Ala Val Ala Gly Val Asn Tyr Ala Asp Gly Ile Phe

450 455 460

Val Trp Leu Thr Arg Leu Gly Glu Glu Asn Val Arg Thr Thr Leu Ser

465 470 475 480

Asn Leu Ala Gln Leu Leu His Ala Ala Arg Tyr Ala Pro His Tyr Thr

485 490 495

Leu Leu His Ala Ala Gln Pro Ala Leu Thr Ser Thr Pro

500 505

<210> 78

<211> 509

<212> PRT

<213> Serratia marcescens UMH2

<400> 78

Met Ala Glu Ser Asn Ala Ala Ile Gln Ser Ala Ala Ile Ile Gly Ala

1 5 10 15

Gly Thr Met Gly Arg Gly Ile Ala Tyr Leu Leu Ala Gln Lys Ser Ile

20 25 30

Arg Thr Val Leu Tyr Asn Arg Asn Gly Asn Thr Leu Asn Gln Ala Arg

35 40 45

Asp Ala Ile Val Gln Asp Leu Asn Lys Lys Val Glu Gln Gly Lys Leu

50 55 60

Ala Leu Gln Asp Lys Asp Ala Val Leu Ala Asn Leu Thr Phe Ser Thr

65 70 75 80

Glu Phe Gly Ala Ile Ala Asp Ser Asp Leu Val Ile Glu Thr Ile Ala

85 90 95

Glu His Glu Gln Ala Lys Leu Glu Val Leu Ala Ala Ile Ala Ala Thr

100 105 110

Val Lys Pro Asp Thr Leu Ile Ala Thr Asn Thr Ser Ser Leu Ser Leu

115 120 125

Asn Lys Leu Ala Thr Ala Val Thr His Ser Glu Arg Phe Ile Gly Leu

130 135 140

His Phe Phe Asn Pro Ala Pro Leu Met Lys Leu Ile Glu Ile Ile Pro

145 150 155 160

Ala Tyr Phe Thr Ala Gln Val Thr Thr Glu Arg Cys Arg Gln Leu Val

165 170 175

Ala Ala Leu Gly Lys Arg Asp Val Val Cys Gln Ala Thr Pro Gly Phe

180 185 190

Ile Val Asn Arg Met Ala Arg Pro Tyr Tyr Leu Glu Gly Phe Arg Leu

195 200 205

Leu Glu Glu His Val Ala Arg Ala Pro Gln Ile Asp Arg Ala Leu Lys

210 215 220

Ala Gly Gly His Phe Arg Met Gly Pro Leu Glu Leu Thr Asp Phe Ile

225 230 235 240

Gly Gln Asp Ile Asn Tyr Gln Val Ser Arg Gln Ile Trp Gln Asp Met

245 250 255

Gln Tyr Asp Pro Arg Tyr Thr Pro Gly His Leu Gln Arg Ser Leu Val

260 265 270

Asp Ala Gly Leu Leu Gly Lys Lys Asn Gly Arg Ser Tyr Phe Ser Ala

275 280 285

Glu Glu Ser Pro Pro Pro Leu Ala Ala Ala Val Asp Ala Glu Val Glu

290 295 300

Ala Leu Arg Ile Tyr Gly Glu His Pro Leu Phe Thr Leu Leu Gln Gln

305 310 315 320

Arg Ala Ala Leu Gln Trp Pro Arg Leu Arg Val Glu Gln Arg Pro Thr

325 330 335

Leu Pro Gly Leu Gly Ala Ala Ile Gln Val Asn Asp Ala Phe Thr Val

340 345 350

Ser Val Thr Asp Gly Arg Thr Ala Asn Gln Leu Ala Glu Gln Thr Ala

355 360 365

Ala Asp Ala Phe Val Val Asp Val Ala Leu Asn Tyr Gly Asp Thr Ala

370 375 380

Tyr Leu Val Ala Ala His Ser Arg His Ala Ser Ala Ala Asn Lys Ala

385 390 395 400

Leu Phe Leu Arg Leu Leu His Thr Ala Ile Pro Gln Val Glu Phe Ile

405 410 415

Lys Asp Ser Pro Ala Leu Ile Val Ala Arg Val Leu Ser Ser Leu Ile

420 425 430

Asn Glu Ser Val Ile Met Val Glu Ser Gly Val Cys Ser Arg Glu Asp

435 440 445

Ile Asp Val Ala Ala Val Ala Gly Val Asn Tyr Ala Asp Gly Ile Phe

450 455 460

Gly Trp Leu Thr His Leu Gly Glu Glu Asn Val Arg Thr Thr Leu Ser

465 470 475 480

Asn Leu Ala Gln Leu Leu His Ala Ala Arg Tyr Ala Pro His Tyr Thr

485 490 495

Leu Leu His Ala Ala Gln Pro Ala Leu Thr Thr Thr Pro

500 505

<210> 79

<211> 509

<212> PRT

<213> Serratia plymuthica AS9

<400> 79

Met Ala Glu Asn Asn Ser Ala Ile Arg Ser Ala Ala Val Ile Gly Ala

1 5 10 15

Gly Thr Met Gly Arg Gly Ile Ala Tyr Leu Leu Ala Leu Asn Gly Ile

20 25 30

Arg Thr Val Leu Tyr Asn Arg Asn Gly Asn Asn Leu Asn Gln Ala Arg

35 40 45

Asp Tyr Ile Val Ser Asp Leu Asp Arg Lys Met Asp Asn Gly Lys Ile

50 55 60

Thr Leu Gln Lys Lys Gly Gln Ile Leu Ala Asn Ile Ile Phe Ser Ala

65 70 75 80

Val Phe Asp Ala Ile Thr Asp Ser Asp Leu Val Ile Glu Thr Ile Ala

85 90 95

Glu Asp Glu Gln Thr Lys His Glu Ile Leu Ala Ala Ile Ala Ala Thr

100 105 110

Val Lys Pro Glu Ala Ile Ile Ala Thr Asn Thr Ser Ser Leu Ser Leu

115 120 125

Asn Lys Leu Ala Ala Gly Val Glu Asn Asn Pro Arg Phe Ile Gly Leu

130 135 140

His Phe Phe Asn Pro Ala Pro Leu Met Lys Leu Ile Glu Ile Ile Pro

145 150 155 160

Ser Tyr Phe Thr Ser His Thr Thr Ser Leu Arg Cys Gln Gln Leu Val

165 170 175

Ile Ala Leu Gly Lys Gln Phe Val Val Cys Lys Ala Thr Pro Gly Phe

180 185 190

Ile Val Asn Arg Met Ala Arg Pro Phe Tyr Leu Glu Gly Phe Arg Leu

195 200 205

Leu Glu Glu Asn Val Ala Gln Ala Pro Gln Ile Asp Arg Ala Leu Lys

210 215 220

Ala Gly Gly His Phe Arg Met Gly Pro Leu Glu Leu Thr Asp Phe Ile

225 230 235 240

Gly Gln Asp Ile Asn Tyr Gln Val Ser Lys Gln Ile Trp Gln Asp Met

245 250 255

Gln Phe Asp Ser Arg Tyr Thr Pro Gly His Leu Gln Arg Ser Leu Val

260 265 270

Asp Ala Gly Leu Leu Gly Arg Lys Asn Gly Arg Ser Phe Phe Ala Ser

275 280 285

Leu Pro Ala Thr Pro Pro Thr Pro Ala Thr Glu Ser Asp Thr Pro Thr

290 295 300

Ser Leu His Phe Tyr Gly Glu His Ala Leu Phe Asp His Leu Gln Gln

305 310 315 320

Arg Ala Leu Ala Thr Trp Pro Ala Leu Arg Val Gln Arg Leu Pro Glu

325 330 335

Arg Pro Glu Leu Gly Arg Leu Ile Leu Val Asn Asn Thr Leu Ala Ile

340 345 350

Lys Ile Thr Asp Gly Arg Thr Ala Asn Leu Leu Ala Gly Leu Thr Ala

355 360 365

Leu Asp Thr Phe Val Ile Asp Ala Ala Leu Asn Tyr Ala Asp Thr Ala

370 375 380

Tyr Leu Val Ala Ala His Asn Gln His Ala Thr Glu Ala Asn Lys Ala

385 390 395 400

Leu Phe Leu Ser Leu Leu Gln Thr Val Ile Ala Gln Val Glu Phe Ile

405 410 415

Lys Asp Ser Pro Ala Leu Ile Val Ala Arg Val Leu Ser Ser Leu Ile

420 425 430

Asn Glu Ser Val Ile Met Val Glu Ser Gly Val Cys Ser Arg Ala Asp

435 440 445

Ile Asp Ile Ala Ala Val Ala Gly Val Asn Tyr Ala Asp Gly Ile Phe

450 455 460

Ala Trp Leu Ala Gln Leu Gly Gln Lys Asn Val Lys Ser Thr Leu Asp

465 470 475 480

Asn Met Ala Gln Leu Leu His Ser Ala Arg Tyr Tyr Pro His Tyr Ser

485 490 495

Leu Leu Asn Ala Ala Arg Pro Glu Leu Ala Val Ala Pro

500 505

<210> 80

<211> 509

<212> PRT

<213> Serratia plymuthica tumat 205

<400> 80

Met Ala Glu Asn Asn Ser Ala Ile Arg Ser Ala Ala Val Ile Gly Ala

1 5 10 15

Gly Thr Met Gly Arg Gly Ile Ala Tyr Leu Leu Ala Leu Asn Gly Ile

20 25 30

Arg Thr Val Leu Tyr Asn Arg Asn Gly Asn Asn Leu Asn Gln Ala Arg

35 40 45

Asp Tyr Ile Val Ser Asp Leu Asp Arg Lys Ile Asp Asn Gly Lys Ile

50 55 60

Thr Leu Gln Lys Lys Gly Gln Ile Leu Ala Asn Ile Ile Phe Ser Ala

65 70 75 80

Val Phe Asp Ala Ile Thr Asp Ser Asp Leu Val Ile Glu Thr Ile Ala

85 90 95

Glu Asp Glu Gln Thr Lys His Glu Ile Leu Ala Ala Ile Ala Ala Thr

100 105 110

Val Lys Pro Glu Ala Ile Ile Ala Thr Asn Thr Ser Ser Leu Ser Leu

115 120 125

Asn Lys Leu Ala Ala Gly Val Glu Asn Asn Pro Arg Phe Ile Gly Leu

130 135 140

His Phe Phe Asn Pro Ala Pro Leu Met Lys Leu Ile Glu Ile Ile Pro

145 150 155 160

Ser Tyr Phe Thr Ser His Ala Thr Ser Leu Arg Cys Gln Gln Leu Val

165 170 175

Ile Ala Leu Gly Lys Gln Phe Val Val Cys Lys Ala Thr Pro Gly Phe

180 185 190

Ile Val Asn Arg Met Ala Arg Pro Phe Tyr Leu Glu Gly Phe Arg Leu

195 200 205

Leu Glu Glu Asn Val Ala Leu Ala Pro Gln Ile Asp Arg Ala Leu Lys

210 215 220

Ala Gly Gly His Phe Arg Met Gly Pro Leu Glu Leu Thr Asp Phe Ile

225 230 235 240

Gly Gln Asp Ile Asn Tyr Gln Val Ser Lys Gln Ile Trp Gln Asp Met

245 250 255

Gln Phe Asp Pro Arg Tyr Thr Pro Gly His Leu Gln Arg Ser Leu Val

260 265 270

Asp Ala Gly Leu Leu Gly Arg Lys Asn Gly Arg Ser Phe Phe Ala Ser

275 280 285

Gln Pro Val Thr Pro Pro Thr Pro Thr Thr Glu Ser Asp Thr Pro Thr

290 295 300

Ser Leu His Phe Tyr Gly Glu His Ala Leu Phe Asp His Leu Gln Gln

305 310 315 320

Arg Ala Leu Ala Thr Trp Pro Ala Leu Arg Val Gln Arg Leu Pro Glu

325 330 335

Arg Pro Glu Leu Gly Arg Phe Ile Leu Val Asn Asn Ala Met Ala Ile

340 345 350

Lys Ile Thr Asp Gly Arg Thr Ala Asn Leu Leu Ala Gly Leu Thr Ala

355 360 365

Leu Asp Thr Phe Val Ile Asp Ala Ala Leu Asn Tyr Ala Asp Thr Ala

370 375 380

Tyr Leu Val Ala Ala His Asn Gln His Ala Thr Glu Thr Asn Lys Ala

385 390 395 400

Leu Phe Leu Thr Leu Leu Gln Thr Val Ile Ala Gln Val Glu Phe Ile

405 410 415

Lys Asp Ser Pro Ala Leu Ile Val Ala Arg Val Leu Ser Ser Leu Ile

420 425 430

Asn Glu Ser Val Ile Met Val Glu Ser Gly Val Cys Ser Arg Ala Asp

435 440 445

Ile Asp Ile Ala Ala Val Ala Gly Val Asn Tyr Ala Asp Gly Ile Phe

450 455 460

Ala Trp Leu Ala Gln Leu Gly Gln Lys Asn Val Lys Ser Thr Leu Asp

465 470 475 480

Asn Met Ala Gln Leu Leu His Ser Ala Arg Tyr Tyr Pro His Tyr Ser

485 490 495

Leu Leu Asn Ala Ala Arg Pro Glu Leu Ala Val Ala Pro

500 505

<210> 81

<211> 509

<212> PRT

<213> Serratia plymuthica A30

<400> 81

Met Ala Glu Asn Asn Ser Ala Ile Arg Ser Ala Ala Val Ile Gly Ala

1 5 10 15

Gly Thr Met Gly Arg Gly Ile Ala Tyr Leu Leu Ala Leu Asn Gly Ile

20 25 30

Arg Thr Val Leu Tyr Asn Arg Asn Gly Asn Asn Leu Asn Gln Ala Arg

35 40 45

Asp Tyr Ile Val Ser Asp Leu Asp Arg Lys Ile Asp Asn Gly Lys Ile

50 55 60

Thr Leu Gln Lys Lys Gly Gln Ile Leu Ala Asn Ile Ile Phe Ser Ala

65 70 75 80

Val Phe Asp Ala Ile Thr Asp Ser Asp Leu Val Ile Glu Thr Ile Ala

85 90 95

Glu Asp Glu Gln Thr Lys His Glu Ile Leu Ala Ala Ile Ala Ala Thr

100 105 110

Val Lys Pro Glu Ala Ile Ile Ala Thr Asn Thr Ser Ser Leu Ser Leu

115 120 125

Asn Lys Leu Ala Ala Gly Val Glu Asn Asn Pro Arg Phe Ile Gly Leu

130 135 140

His Phe Phe Asn Pro Ala Pro Leu Met Lys Leu Ile Glu Ile Ile Pro

145 150 155 160

Ser Tyr Phe Thr Ser His Ala Thr Ser Leu Arg Cys Gln Lys Leu Val

165 170 175

Ile Ala Leu Gly Lys Gln Phe Val Val Cys Lys Ala Thr Pro Gly Phe

180 185 190

Ile Val Asn Arg Met Ala Arg Pro Phe Tyr Leu Glu Gly Phe Arg Leu

195 200 205

Leu Glu Glu Asn Val Ala Leu Ala Pro Gln Ile Asp Arg Ala Leu Lys

210 215 220

Ala Gly Gly His Phe Arg Met Gly Pro Leu Glu Leu Thr Asp Phe Ile

225 230 235 240

Gly Gln Asp Ile Asn Tyr Gln Val Ser Lys Gln Ile Trp Gln Asp Met

245 250 255

Gln Phe Asp Pro Arg Tyr Thr Pro Gly His Leu Gln Arg Ser Leu Val

260 265 270

Asp Ala Gly Leu Leu Gly Arg Lys Asn Gly Arg Ser Phe Phe Ala Ser

275 280 285

Gln Pro Ala Thr Pro Pro Asn Pro Thr Thr Glu Gly Asp Thr Pro Thr

290 295 300

Ser Leu His Phe Tyr Gly Glu His Ala Leu Phe Asp His Leu Gln Gln

305 310 315 320

Arg Ala Leu Ala Thr Trp Pro Ala Leu Arg Val Gln Arg Leu Pro Glu

325 330 335

Arg Pro Glu Leu Gly Arg Phe Ile Leu Met Asn Asn Arg Leu Ala Ile

340 345 350

Lys Ile Thr Asp Gly Arg Thr Ala Asn Leu Leu Ala Gly Leu Thr Ala

355 360 365

Leu Asp Thr Phe Val Ile Asp Ala Ala Leu Asn Tyr Ala Asp Thr Ala

370 375 380

Tyr Leu Val Ala Ala His Asn Gln His Ala Thr Glu Thr Asn Lys Ala

385 390 395 400

Leu Phe Leu Thr Leu Leu Gln Thr Leu Ile Ala Gln Val Glu Phe Ile

405 410 415

Lys Asp Ser Pro Ala Leu Ile Val Ala Arg Val Leu Ser Ser Leu Ile

420 425 430

Asn Glu Ser Val Ile Met Val Glu Ser Gly Val Cys Ser Arg Ala Asp

435 440 445

Ile Asp Ile Ala Ala Val Ala Gly Val Asn Tyr Ala Asp Gly Ile Phe

450 455 460

Ala Trp Leu Ala Gln Leu Gly Gln Lys Asn Val Lys Ser Thr Leu Asp

465 470 475 480

Asn Met Ala Gln Leu Leu His Ser Ala Arg Tyr Tyr Pro His Tyr Ser

485 490 495

Leu Leu Asn Ala Ala Arg Pro Glu Leu Ala Val Ala Pro

500 505

<210> 82

<211> 509

<212> PRT

<213> Serratia plymuthica 4Rx13

<400> 82

Met Ala Glu Asn Asn Ser Ala Ile Arg Ser Ala Ala Val Ile Gly Ala

1 5 10 15

Gly Thr Met Gly Arg Gly Ile Ala Tyr Leu Leu Ala Leu Asn Gly Ile

20 25 30

Arg Thr Val Leu Tyr Asn Arg Asn Gly Asn Ser Leu Asn Gln Ala Arg

35 40 45

Asp Tyr Ile Val Ser Asp Leu Asp Arg Lys Ile Asp Asn Gly Lys Ile

50 55 60

Thr Leu Gln Lys Lys Gly Gln Ile Leu Ala Asn Ile Ile Phe Ser Ala

65 70 75 80

Val Phe Asp Ala Ile Thr Asp Ser Asp Leu Val Ile Glu Thr Ile Ala

85 90 95

Glu Asp Glu Gln Thr Lys His Glu Ile Leu Ala Ala Ile Ala Ala Thr

100 105 110

Val Lys Pro Glu Ala Ile Ile Ala Thr Asn Thr Ser Ser Leu Ser Leu

115 120 125

Asn Lys Leu Ala Ala Gly Val Glu Asn Asn Pro Arg Phe Ile Gly Leu

130 135 140

His Phe Phe Asn Pro Ala Pro Leu Met Lys Leu Ile Glu Ile Ile Pro

145 150 155 160

Ser Tyr Phe Thr Ser His Ala Thr Ser Leu Arg Cys Gln Lys Leu Val

165 170 175

Ile Ala Leu Gly Lys Gln Phe Val Val Cys Lys Ala Thr Pro Gly Phe

180 185 190

Ile Val Asn Arg Met Ala Arg Pro Phe Tyr Leu Glu Gly Phe Arg Leu

195 200 205

Leu Glu Glu Asn Val Ala Leu Ala Pro Gln Ile Asp Arg Ala Leu Lys

210 215 220

Ala Gly Gly His Phe Arg Met Gly Pro Leu Glu Leu Thr Asp Phe Ile

225 230 235 240

Gly Gln Asp Ile Asn Tyr Gln Val Ser Lys Gln Ile Trp Gln Asp Met

245 250 255

Gln Phe Asp Pro Arg Tyr Thr Pro Gly His Leu Gln Arg Ser Leu Val

260 265 270

Asp Ala Gly Leu Leu Gly Arg Lys Asn Gly Arg Ser Phe Phe Ala Ser

275 280 285

Gln Pro Ala Thr Pro Pro Thr Pro Thr Thr Glu Ser Asp Thr Pro Thr

290 295 300

Ser Leu His Phe Tyr Gly Glu His Ala Leu Phe Asp His Leu Gln Gln

305 310 315 320

Arg Ala Leu Ala Thr Trp Pro Ala Leu Ser Val Gln Arg Leu Pro Glu

325 330 335

Arg Pro Glu Leu Gly Arg Phe Ile Leu Val Asn Asn Ala Leu Ala Ile

340 345 350

Lys Ile Thr Asp Gly Arg Thr Ala Asn Leu Leu Ala Gly Leu Thr Ala

355 360 365

Leu Asp Thr Phe Val Ile Asp Ala Ala Leu Asn Tyr Ala Asp Thr Ala

370 375 380

Tyr Leu Val Ala Ala His Asn Gln His Ala Thr Glu Thr Asn Lys Ala

385 390 395 400

Leu Phe Leu Thr Leu Leu Gln Thr Val Ile Ala Gln Val Glu Phe Ile

405 410 415

Lys Asp Ser Pro Ala Leu Ile Val Ala Arg Val Leu Ser Ser Leu Ile

420 425 430

Asn Glu Ser Val Ile Met Val Glu Ser Gly Val Cys Ser Arg Ala Asp

435 440 445

Ile Asp Ile Ala Ala Val Ala Gly Val Asn Tyr Ala Asp Gly Ile Phe

450 455 460

Ala Trp Leu Ala Gln Leu Gly Gln Lys Asn Val Lys Ser Thr Leu Asp

465 470 475 480

Asn Met Ala Gln Leu Leu His Ser Ala Arg Tyr Tyr Pro His Tyr Ser

485 490 495

Leu Leu Asn Ala Ala Arg Pro Glu Leu Ala Val Ala Pro

500 505

<210> 83

<211> 509

<212> PRT

<213> Serratia plymuthica V4

<400> 83

Met Ala Glu Asn Asn Ser Ala Ile Arg Ser Ala Ala Val Ile Gly Ala

1 5 10 15

Gly Thr Met Gly Arg Gly Ile Ala Tyr Leu Leu Ala Leu Asn Gly Ile

20 25 30

Arg Thr Val Leu Tyr Asn Arg Asn Gly Asn Asn Leu Asn Gln Ala Arg

35 40 45

Asp Tyr Ile Val Ser Asp Leu Asp Arg Lys Ile Asp Asn Gly Lys Ile

50 55 60

Thr Leu Gln Lys Lys Gly Gln Ile Leu Ala Asn Ile Ile Phe Ser Ala

65 70 75 80

Val Phe Asp Ala Ile Thr Asp Ser Asp Leu Val Ile Glu Thr Ile Ala

85 90 95

Glu Asp Glu Gln Thr Lys His Glu Ile Leu Ala Ala Ile Ala Ala Thr

100 105 110

Val Lys Pro Glu Ala Ile Ile Ala Thr Asn Thr Ser Ser Leu Ser Leu

115 120 125

Asn Lys Leu Ala Ala Gly Val Glu Asn Asn Pro Arg Phe Ile Gly Leu

130 135 140

His Phe Phe Asn Pro Ala Pro Leu Met Lys Leu Ile Glu Ile Ile Pro

145 150 155 160

Ser Tyr Phe Thr Ser His Ala Thr Ser Leu Arg Cys Gln Lys Leu Val

165 170 175

Ile Ala Leu Gly Lys Gln Phe Val Val Cys Lys Ala Thr Pro Gly Phe

180 185 190

Ile Val Asn Arg Met Ala Arg Pro Phe Tyr Leu Glu Gly Phe Arg Leu

195 200 205

Leu Glu Glu Asn Val Ala Leu Ala Pro Gln Ile Asp Arg Ala Leu Lys

210 215 220

Ala Gly Gly His Phe Arg Met Gly Pro Leu Glu Leu Thr Asp Phe Ile

225 230 235 240

Gly Gln Asp Ile Asn Tyr Gln Val Ser Lys Gln Ile Trp Gln Asp Met

245 250 255

Gln Phe Asp Pro Arg Tyr Thr Pro Gly His Leu Gln Arg Ser Leu Val

260 265 270

Asp Ala Gly Leu Leu Gly Arg Lys Asn Gly Arg Ser Phe Phe Ala Ser

275 280 285

Gln Pro Ala Thr Pro Pro Thr Pro Thr Thr Glu Ser Asp Thr Pro Thr

290 295 300

Ser Leu His Phe Tyr Gly Glu His Ala Leu Phe Asp His Leu Gln Gln

305 310 315 320

Arg Ala Leu Ala Thr Trp Pro Ala Leu Arg Val Gln Arg Leu Pro Glu

325 330 335

Arg Pro Glu Leu Gly Arg Phe Ile Leu Val Asn Asn Arg Leu Ala Ile

340 345 350

Lys Ile Thr Asp Gly Arg Thr Ala Asn Leu Leu Ala Gly Leu Thr Ala

355 360 365

Leu Asp Thr Phe Val Ile Asp Ala Ala Leu Asn Tyr Ala Asp Thr Ala

370 375 380

Tyr Leu Val Ala Ala His Asn Gln His Ala Thr Glu Thr Asn Lys Ala

385 390 395 400

Leu Phe Leu Thr Leu Leu Gln Thr Val Ile Ala Gln Val Glu Phe Ile

405 410 415

Lys Asp Ser Pro Ala Leu Ile Val Ala Arg Val Leu Ser Ser Leu Ile

420 425 430

Asn Glu Ser Val Ile Met Val Glu Ser Gly Val Cys Ser Arg Ala Asp

435 440 445

Ile Asp Ile Ala Ala Val Ala Gly Val Asn Tyr Ala Asp Gly Ile Phe

450 455 460

Ala Trp Leu Ala Gln Leu Gly Gln Lys Asn Val Lys Ser Thr Leu Asp

465 470 475 480

Asn Met Ala Gln Leu Leu His Ser Ala Arg Tyr Tyr Pro His Tyr Ser

485 490 495

Leu Leu Asn Ala Ala Arg Pro Glu Leu Ala Val Ala Pro

500 505

<210> 84

<211> 509

<212> PRT

<213> Serratia plymuthica 3Rp8

<400> 84

Met Ala Glu Asn Asn Ser Ala Ile Arg Ser Ala Ala Val Ile Gly Ala

1 5 10 15

Gly Thr Met Gly Arg Gly Ile Ala Tyr Leu Leu Ala Leu Asn Gly Ile

20 25 30

Arg Thr Val Leu Tyr Asn Arg Asn Gly Asn Asn Leu Asn Gln Ala Arg

35 40 45

Asp Tyr Ile Val Ser Asp Leu Asp Arg Lys Ile Asp Asn Gly Lys Ile

50 55 60

Thr Leu Gln Lys Lys Gly Gln Ile Leu Ala Asn Ile Ile Phe Ser Ala

65 70 75 80

Val Phe Asp Ala Ile Thr Asp Ser Asp Leu Val Ile Glu Thr Ile Ala

85 90 95

Glu Asp Glu Gln Thr Lys His Glu Ile Leu Ala Ala Ile Ala Ala Thr

100 105 110

Val Lys Pro Glu Ala Ile Ile Ala Thr Asn Thr Ser Ser Leu Ser Leu

115 120 125

Asn Lys Leu Ala Ala Gly Val Glu Asn Asn Pro Arg Phe Ile Gly Leu

130 135 140

His Phe Phe Asn Pro Ala Pro Leu Met Lys Leu Ile Glu Ile Ile Pro

145 150 155 160

Ser Tyr Phe Thr Ser His Ala Thr Ser Leu Arg Cys Gln Lys Leu Val

165 170 175

Ile Ala Leu Gly Lys Gln Phe Val Val Cys Lys Ala Thr Pro Gly Phe

180 185 190

Ile Val Asn Arg Met Ala Arg Pro Phe Tyr Leu Glu Gly Phe Arg Leu

195 200 205

Leu Glu Glu Asn Val Ala Leu Ala Pro Gln Ile Asp Arg Ala Leu Lys

210 215 220

Ala Gly Gly His Phe Arg Met Gly Pro Leu Glu Leu Thr Asp Phe Ile

225 230 235 240

Gly Gln Asp Ile Asn Tyr Gln Val Ser Lys Gln Ile Trp Gln Asp Met

245 250 255

Gln Phe Asp Pro Arg Tyr Thr Pro Gly His Leu Gln Arg Ser Leu Val

260 265 270

Asp Ala Gly Leu Leu Gly Arg Lys Asn Gly Arg Ser Phe Phe Ala Ser

275 280 285

Gln Pro Ala Thr Pro Pro Thr Pro Thr Thr Glu Ser Asp Thr Pro Thr

290 295 300

Ser Leu His Phe Tyr Gly Glu His Ala Leu Phe Asp His Leu Gln Gln

305 310 315 320

Arg Ala Leu Ala Thr Trp Pro Ala Leu Arg Val Gln Arg Leu Pro Glu

325 330 335

Arg Pro Glu Leu Gly Arg Phe Ile Leu Val Asn Asn Arg Leu Ala Ile

340 345 350

Lys Ile Thr Asp Gly Arg Thr Ala Asn Leu Leu Ala Gly Leu Thr Ala

355 360 365

Leu Asp Thr Phe Val Ile Asp Ala Ala Leu Asn Tyr Ala Asp Thr Ala

370 375 380

Tyr Leu Val Ala Ala His Asn Gln His Ala Thr Glu Thr Asn Lys Ala

385 390 395 400

Leu Phe Leu Thr Leu Leu Gln Thr Leu Ile Ala Gln Val Glu Phe Ile

405 410 415

Lys Asp Ser Pro Ala Leu Ile Val Ala Arg Val Leu Ser Ser Leu Ile

420 425 430

Asn Glu Ser Val Ile Met Val Glu Ser Gly Val Cys Ser Arg Ala Asp

435 440 445

Ile Asp Ile Ala Ala Val Ala Gly Val Asn Tyr Ala Asp Gly Ile Phe

450 455 460

Ala Trp Leu Ala Gln Leu Gly Gln Lys Asn Val Lys Ser Thr Leu Asp

465 470 475 480

Asn Met Ala Gln Leu Leu His Ser Ala Arg Tyr Tyr Pro His Tyr Ser

485 490 495

Leu Leu Asn Ala Ala Arg Pro Glu Leu Ala Val Ala Pro

500 505

<210> 85

<211> 509

<212> PRT

<213> Serratia proteamaculans MFPA44A14

<400> 85

Met Ala Glu Asn Asn Ser Ala Ile His Ser Val Ala Val Ile Gly Ala

1 5 10 15

Gly Thr Met Gly Arg Gly Ile Ala Tyr Leu Leu Ala Gln Asn Gly Ile

20 25 30

Arg Thr Leu Leu Tyr Asn Arg Ser Gly Asn Asn Leu Asp Gln Ala Arg

35 40 45

Asp Tyr Ile Ile Arg Asp Leu Asp Lys Lys Ile Asp Asn Gly Lys Ile

50 55 60

Ser Pro Gln Lys Lys Gly Glu Val Leu Ala Asn Leu Val Phe Ser Pro

65 70 75 80

Ile Phe Asp Ala Ile Ala Asp Ser Asp Leu Val Ile Glu Thr Ile Ala

85 90 95

Glu His Glu Thr Thr Lys His Glu Ile Leu Ala Ala Ile Ala Ala Thr

100 105 110

Val Lys Gln Glu Ala Ile Ile Ala Thr Asn Thr Ser Ser Leu Ser Leu

115 120 125

Asn Lys Leu Ala Ala Gly Val Glu Asn Asn Ala Arg Phe Ile Gly Leu

130 135 140

His Phe Phe Asn Pro Ala Pro Leu Met Lys Leu Ile Glu Ile Ile Pro

145 150 155 160

Ser Tyr Phe Thr Ser Arg Ala Thr Ser Leu Arg Cys Gln Gln Leu Val

165 170 175

Thr Ala Leu Gly Lys Gln Phe Val Val Cys Lys Ala Thr Pro Gly Phe

180 185 190

Ile Val Asn Arg Met Ala Arg Pro Phe Tyr Leu Glu Gly Phe Arg Leu

195 200 205

Leu Glu Glu Asn Val Ala Leu Ala Pro Gln Ile Asp Arg Ala Leu Lys

210 215 220

Ala Gly Gly His Phe Arg Met Gly Pro Leu Glu Leu Thr Asp Phe Ile

225 230 235 240

Gly Gln Asp Ile Asn Tyr Gln Val Ser Ser Gln Ile Trp Gln Asp Met

245 250 255

Gln Tyr Asp Pro Arg Tyr Thr Pro Gly His Leu Gln Arg Ser Leu Val

260 265 270

Asp Ala Gly Leu Leu Gly Lys Lys Asn Gly Arg Ser Phe Phe Ala Ala

275 280 285

Pro Ser Ala Glu Ser Asn Leu Leu Asp Ala Gly Asn Gly Thr Leu Thr

290 295 300

Ser Leu His Phe Tyr Gly Glu His Thr Leu Phe Asp Leu Leu Gln Gln

305 310 315 320

Arg Ala Leu Ala Thr Trp Pro Thr Leu Gln Ile Ile His Gln Pro Glu

325 330 335

Arg Pro Thr Leu Gly Arg Phe Ile Arg Val Asn Asp Ala Leu Ala Val

340 345 350

Lys Ile Thr Asp Gly Arg Thr Ala Asn Leu Leu Ala Glu Leu Thr Asp

355 360 365

Leu Asp Thr Phe Val Ile Asp Ala Ala Leu Asn Tyr Ser Asp Thr Thr

370 375 380

Tyr Leu Val Ala Ala His Asn Gln Asp Ala Ala Glu Ala Asn Lys Ala

385 390 395 400

Leu Phe Leu Ser Leu Leu Gln Thr Leu Ile Pro Gln Val Glu Phe Ile

405 410 415

Lys Asp Ser Pro Gly Leu Ile Val Ala Arg Val Leu Ser Ser Leu Ile

420 425 430

Asn Glu Ser Val Ile Met Val Glu Ser Gly Val Cys Ser Arg Ala Asp

435 440 445

Ile Asp Ile Ala Ala Val Ala Gly Val Asn Tyr Ala Asp Gly Ile Phe

450 455 460

Ala Trp Leu Thr Gln Leu Gly Gln Lys Asn Val Lys Ser Thr Leu Asp

465 470 475 480

Asn Met Ala Gln Leu Leu His Ser Ala Arg Tyr Tyr Pro His Tyr Ser

485 490 495

Leu Leu Asn Ile Pro Arg Pro Glu Leu Ala Val Ala Pro

500 505

<210> 86

<211> 509

<212> PRT

<213> Serratia plymuthica A153

<400> 86

Met Ala Glu Asn Asn Ser Ala Ile Arg Ser Ala Ala Val Ile Gly Ala

1 5 10 15

Gly Thr Met Gly Arg Gly Ile Ala Tyr Leu Leu Ala Leu Asn Gly Ile

20 25 30

Arg Thr Val Leu Tyr Asn Arg Asn Gly Asn Asn Leu Asn Gln Ala Arg

35 40 45

Asp Tyr Ile Val Ser Asp Leu Asp Arg Lys Ile Asp Asn Gly Lys Ile

50 55 60

Thr Leu Gln Lys Lys Gly Gln Ile Leu Ala Asn Ile Ile Phe Ser Asp

65 70 75 80

Val Phe Asp Ala Ile Thr Asp Ser Asp Leu Val Ile Glu Thr Ile Ala

85 90 95

Glu Asp Glu Gln Thr Lys His Glu Ile Leu Ala Ala Ile Ala Ala Thr

100 105 110

Val Lys Pro Glu Ala Ile Ile Ala Thr Asn Thr Ser Ser Leu Ser Leu

115 120 125

Asn Lys Leu Ala Ala Gly Val Glu Asn Asn Pro Arg Phe Ile Gly Leu

130 135 140

His Phe Phe Asn Pro Ala Pro Leu Met Lys Leu Ile Glu Ile Ile Pro

145 150 155 160

Ser Tyr Phe Thr Ser Arg Ala Thr Ser Leu Arg Cys Gln Gln Leu Val

165 170 175

Thr Ala Leu Gly Lys Leu Phe Val Val Cys Lys Ala Thr Pro Gly Phe

180 185 190

Ile Val Asn Arg Met Ala Arg Pro Phe Tyr Leu Glu Gly Phe Arg Leu

195 200 205

Leu Glu Glu Asn Val Ala Leu Ala Pro Gln Ile Asp Arg Ala Leu Lys

210 215 220

Ala Gly Gly His Phe Arg Met Gly Pro Leu Glu Leu Thr Asp Phe Ile

225 230 235 240

Gly Gln Asp Ile Asn Tyr Gln Val Ser Lys Gln Ile Trp Gln Asp Met

245 250 255

Gln Phe Asp Pro Arg Tyr Thr Pro Gly His Leu Gln Arg Ser Leu Val

260 265 270

Asp Ala Gly Leu Leu Gly Arg Lys Asn Gly Arg Ser Phe Phe Ala Ser

275 280 285

Gln Pro Ala Thr Pro Pro Asn Pro Thr Thr Glu Gly His Thr Pro Thr

290 295 300

Ser Leu Leu Phe Tyr Gly Glu His Ala Leu Phe Asp His Leu Gln Gln

305 310 315 320

Arg Ala Leu Ala Thr Trp Pro Ala Leu Arg Val Gln Arg Leu Pro Glu

325 330 335

Arg Pro Glu Leu Gly Arg Phe Ile Leu Val Asn Asn Arg Leu Ala Ile

340 345 350

Lys Ile Thr Asp Gly Arg Thr Ala Asn Leu Leu Ala Gly Leu Thr Ala

355 360 365

Leu Asp Thr Phe Val Ile Asp Ala Ala Leu Asn Tyr Ala Asp Thr Ala

370 375 380

Tyr Leu Val Ala Ala His Asn Gln His Ala Thr Glu Pro Asn Lys Ala

385 390 395 400

Leu Phe Leu Thr Leu Leu Gln Thr Leu Ile Ala Gln Val Glu Phe Ile

405 410 415

Lys Asp Ser Pro Ala Leu Ile Val Ala Arg Val Leu Ser Ser Leu Ile

420 425 430

Asn Glu Ser Val Ile Met Val Glu Ser Gly Val Cys Ser Arg Ala Asp

435 440 445

Ile Asp Ile Ala Ala Val Ala Gly Val Asn Tyr Ala Asp Gly Ile Phe

450 455 460

Ala Trp Leu Ala Gln Leu Gly Gln Lys Asn Val Lys Ser Thr Leu Asp

465 470 475 480

Asn Met Ala Gln Leu Leu His Ser Thr Arg Tyr Tyr Pro His Tyr Ser

485 490 495

Leu Leu Asn Ala Ala Arg Pro Glu Leu Ala Val Ala Pro

500 505

<210> 87

<211> 1530

<212> DNA

<213> Serratia marcescens ATCC13880

<400> 87

atggcagaaa gtaatgcggc aattcaatcg gctgcgatta tcggcgcggg aacgatgggc 60

agaggcatcg cttatctttt cgcgcaaaaa ggcattcgca cggtgcttta taatcgcaac 120

ggcaataccc tcaatcaggc tcgcgaatat atcgcgcaag acctgaacaa gaaagtcgaa 180

cagggcaaga tcgcgctgca ggataaaggc gcggtgctgg ccaatctaat gttcacttca 240

gtgtttgagg ccatcgccga cagcgagctg gtgatagaaa ccatcgccga gcaagaacaa 300

accaaacttg aggtgctggc ggccatcgcc gcggtggtca agcccgacac gctgatcgcc 360

accaatacct cctcactgtc gcttaacaag ctggctactg cggtgacgca cagcgaacgc 420

tttatcggtt tgcatttttt caaccccgcg ccgctgatga agctgattga aatcattccg 480

gcctacttta ccgcgcacgc caccaccgaa cgctgccgcc aactggtggc ggcgttgggg 540

aaacacgatg tcgtctgcca ggccacgccg gggttcatcg tcaatcgcat ggcccgcccc 600

tactacctgg aagggttccg cctgttggaa gaacacgtgg cgcgcgcggc tcagatcgac 660

cgcgccctca aggccggcgg gcgcttccgc atggggccgc tcgagctgac cgattttatc 720

ggccaagaca tcaactatca ggtcagtcgg caaatctggc aggatatgca atacgacccg 780

cgctataccc ccggtcatct gcagcgttca ctggtcgatg ccggtctgtt ggggaaaaag 840

aacggccgct cctattttgc cgccgaagaa accgccccgc cggtgacggc cgccagcaat 900

gcagacgtcg agacgctgcg cgtttacggc gagcaccctt tttttaccct gttacagcag 960

cgagccgcgc ttcagtggcc acagctgcgc gtggaacaac ggccggcatt accggggctg 1020

gggtcggccg tccagatcaa tgacgctttc accgtcagca tcaccgatgg ccgcacggcg 1080

agccaactgg ccgagcagac ggcagcggat gcctttgtgg tcgatgtcgc cctgaactac 1140

gccgacacga cgtatctggc ggcggcgcac agccgccacg cctctgcggc caataaggcg 1200

ctgtttttac gcctgctgca cacggcaatc ccgcaggttg aatttatcaa ggactctccg 1260

gcgcttatcg tcgcccgcgt cctcagcagc ctgatcaatg agtcggtgat catggtggaa 1320

agcggcgtct gcagccggga agacatcgat gtcgccgccg tcgcgggcgt taactacgcc 1380

ggcggcattt tcgactggct cggcaaactg ggggagaaaa acgtcaggac aacgctgagc 1440

aatctggctc agctgctgca cgcggcgcgc tatgcgccgc attacaccct tctgcacgcc 1500

gcgcaaccgg cgctgacgac cacgccttaa 1530

<210> 88

<211> 1530

<212> DNA

<213> Serratia nematodiphila DSM21420

<400> 88

atggcagaaa gtaatgcggc aattcaatcg gctgcgatta tcggcgcggg aacgatgggc 60

agaggcatcg cttatctttt cgcgcaaaaa ggcattccca cgatgcttta taatcgcaac 120

ggcaataccc tcaatcaagc tcgcgaatat atcgcgcaag acctgaataa gaaagtcgaa 180

cagggcaaga tcgcgctgcg ggataaagac gcggtgctgg ccaatctgat gttcacttcc 240

gtgtttgagg ccatcgccga cagcgagctg gtgatagaaa ccatcgccga gcaagaacaa 300

accaaacttg aggtgctggc ggccatcgcc gcagtggtca agcccgacac gctgatcgcc 360

accaatacct cctcactgtc gctcaacaag ctggcaacgg cggtaacgca cagcgaacgc 420

tttatcggtt tgcatttttt caaccccgcg ccgctgatga agctgattga aatcattccg 480

gcctacttta ccgcgcacgc caccacggaa cgctgccgcc aactggtggc ggcgttgggg 540

aaacgcgatg tcgtctgcca ggccacgccg gggttcatcg tcaatcgcat ggcccgcccc 600

tactacctgg aagggttccg cctgttggaa gaacatgtgg cgcgcgcggc gcagatcgac 660

cgcgccctca aagccggcgg gcgcttccgc atggggccgc tcgagctgac cgattttatc 720

ggccaagaca tcaactatca ggtcagccgg caaatctggc aggacatgca atacgacccg 780

cgctataccc ctggtcatct gcagcgttca ctggtcgatg ccggtctgtt ggggaaaaag 840

aacggccgct cctattttgc cgccgaagaa accgccccgc cggtgacggc cgccaacaat 900

gcagacgtcg agacgctgcg cgtttacggc gagcatcctt tttttaccct gttgcagcag 960

cgagccgcgc ttcagtggcc acagctgcgc gtggaacaac ggccggcatt accggggctg 1020

ggagcggccg tccagatcaa tgacgctttc accgtcagca tcaccgatgg ccgcacggcg 1080

agccaactgg ccgagcagac ggcagcggat gcctttgtgg tcgatctcgc cctgaactac 1140

gccgacacca cgtatctggt ggcggcgcac agccgccacg cttctgcggc caataaggcg 1200

ctgtttttac gcctgctgca cacggcaatc cctcaggttg aatttatcaa ggactctccg 1260

gcgcttatcg tcgcccgcgt cctcagcagc ctgatcaatg agtcggtgat catggtggaa 1320

agcggcgtct gcagccggga agacatcgat gtcgccgccg tcgcgggcgt taactacgcc 1380

ggcggcattt tcgactggct cggcaaactg ggggagaaaa acgtcaggac gacgctgagc 1440

aatctggcgc agctgctgca cgcggcgcgc tatgcgccgc attacaccct tctgcacgcc 1500

gcgcaaccgg cgctgacgac cacgccttaa 1530

<210> 89

<211> 1530

<212> DNA

<213> Serratia plymuthica NBRC102599

<400> 89

atggcagaga ataattcggc aatccgttca gccgccgtta ttggtgcggg gaccatgggc 60

agaggcatcg cctatctcct ggcgctgaac ggcatacgaa ccgtacttta taatcgcaat 120

ggtaataatc tcaatcaggc ccgtgactat attgtcagcg acctggacag aaaaatagat 180

aacggaaaaa taaccctgca gaaaaaaggc cagatattag ccaatattat tttctcggac 240

gtctttgacg ccataaccga cagcgatctg gtgattgaaa ccattgcgga agatgagcaa 300

accaagcatg aaatcctggc agccattgcg gcgacggtaa aaccggaggc gatcattgcg 360

accaatactt cctctctgtc gctgaacaaa ctggcggcgg gggtggaaaa caacccgcgc 420

tttatcggcc tgcatttttt caatccggcg ccgctgatga agttgatcga aattattccc 480

tcttatttca cctctcgcgc caccagtcta cgctgccagc agttggtaac agcgttgggt 540

aaacagtttg tggtctgcaa agccacgccg ggctttattg ttaaccgcat ggcgcggcct 600

ttctatctgg aagggttccg gctgctggag gaaaacgtgg cgctggcgcc acagatcgac 660

cgcgccctca aggccggcgg gcattttcgc atggggcctt tagaactgac tgattttatc 720

ggccaggata tcaactatca ggtcagcaag cagatttggc aggatatgca gttcgatccc 780

cgctataccc ccggccattt gcaacgctcg ctggtggatg ccgggctgct ggggaggaaa 840

aacgggcgct ctttttttgc ttcccaacct gctacaccac ccaacccaac cacagagggc 900

gacacgccaa cttcactgca tttttatggt gaacacgctt tattcgatca cctgcaacag 960

cgcgctttgg ccacctggcc tgcgctgcgc gttcagcggt tgccggaacg gccggaactg 1020

gggcgtttta tcctggtgaa taacaggctg gcgatcaaaa tcactgatgg cagaacggcg 1080

aacctgctcg ccggcttaac cgctctcgac accttcgtga ttgacgccgc gctgaactac 1140

gccgacaccg cctatctggt ggccgcccac aatcaacatg ccacagagac gaataaagcg 1200

ctgtttctga cgctgctgca aaccctcatc gctcaggtgg agtttattaa agattcccct 1260

gccctgatcg ttgcccgcgt actgagcagc ctgatcaatg aatcggtgat catggtggag 1320

agcggcgttt gcagccgggc agatatcgat atcgccgccg tggccggcgt gaactatgcc 1380

gacggcattt ttgcctggtt ggcgcagctc gggcagaaaa acgtgaaatc gacgctggat 1440

aacatggcgc aattgctgca ctccacgcgc tattacccgc attactcatt gctgaacgcg 1500

gcccggcctg agctggctgt agcgccctaa 1530

<210> 90

<211> 1530

<212> DNA

<213> Serratia proteamaculans 568

<400> 90

atggcagaga ataattcggc aatccattcg gtcgctgtta ttggtgccgg gaccatgggc 60

agaggtattg cctatcttct ggcgcaaaac ggcatacgaa ccctgcttta taatcgtagc 120

ggtaataatc tgaatcaggc cagggactat attattcgtg acctggataa gaaaatagat 180

ggcggaaaaa taagcccgca gaaaaaaggc gagatattgg ccaatctggt tttctctccc 240

attttcgagg ctattgccga cagcgatctg gtgattgaaa ccatcgcgga acatgaggca 300

accaagcatg agatcctcgc ggcgattgcg gccacggtga aaaaagaggc gattatcgcc 360

accaatacct cctcgctgtc attgaataag ctggccgcag gcgtcgaaaa taacgcccgg 420

tttatcggcc tgcacttctt caatccggcc ccgctgatga aactgatcga aattattccg 480

tcttacttta ccagccgggc caccagcctg cgttgccagc agttggtgac ggcgataggc 540

aaacagtttg tggtctgcaa agccacgccg ggctttatcg tcaaccggat ggcgcgacct 600

ttttatctgg aagggttccg gctgttggaa gagaacgtgg cgctggcgcc gcagatcgac 660

cgcgcactca aggccggtgg ccactttcgc atgggacctt tagagctgac cgattttatc 720

ggccaggata ttaactatca ggtcagcagc cagatttggc aggacatgca gtacgatccc 780

cgctataccc ccggccattt gcaacgttcg ctggtggatg ccgggctgct ggggaagaaa 840

aacggccgat ccttttttac cccctcttcc gccgaaccca gctccgccga tgcaggtagc 900

ggcacgccga cctcactgaa tttttatggt gaacatcccc tgttcgacct gttgcaacag 960

cgcgctttgg cgctctggcc aagggtgcag attaatcgcc aatcggaaca gccaacgctg 1020

ggccgcttta tccgggtgaa tgacgcaatg gccatcaaaa tcaccgatgg ccgcaccgcc 1080

aatctgctgg ctgaattgac cgaactcgat accttcgtga tcgacgccgc gctcaactac 1140

gccgataccg cctatctggc ggctgcccac agccaggatg ccagcgcggc caataaagcg 1200

ctgtttctga cgctgctgca aacgttgatc ccgcaggtgg agtttattaa agactccccg 1260

ggcctgattg tcgcccgcgt cctgagcagc ctgatcaatg agtcggtgat tatggtggag 1320

agcggggttt gcagccgggc ggacatcgat atcgccgcgg tggccggcgt taactatgcc 1380

gatggcatct ttagctggct ggcgcagctt gggcaaaaaa acgtgaagtc gacgctggac 1440

aatatggcgc aactgctgca ttccgcccgc tattacccgc attactcttt gctcaatacc 1500

ccccggccag agctggccgt cgcgccctaa 1530

<210> 91

<211> 1530

<212> DNA

<213> Serratia ureilytica Lr5/4

<400> 91

atggcagaaa gtaatgcggc aattcaatcg gctgcgatta tcggcgcggg aacgatgggc 60

agaggcatcg cttatctttt ggcgcaaaaa agcattcgta cggtgcttta taatcgcaac 120

ggcaataccc tcaatcaagc tcgcgacgct atcgtgcaag acctgaacaa aaaggtggaa 180

cagggcaagc tcgcgctgca ggacaaagac gcggtgctgg ctaatctgac gttctccgcg 240

gaatttgggg ccatcgccga cagcgatctg gtgatcgaaa ccatcgcaga gcatgagcaa 300

gccaaactcg aggtgctggc ggccatcgcc gcgacggtca agcccgacac gctgatcgcc 360

accaatacct cctcactgtc gcttaataag ctggcgacgg cggtgacgca cagcgaacgc 420

tttatcggtt tgcacttttt caaccccgcg ccgctgatga agctgattga aatcattccg 480

gcctatttta ccgcacaggt caccaccgaa cgttgccgtc aactggtggc ggcgttgggg 540

aaacgcgatg tcgtctgtca ggccacgccg ggatttatcg tcaaccgtat ggcccgcccc 600

tactatctgg aagggttccg cctgttggaa gaacacgtgg cgcgtgcgcc gcagatcgac 660

cgcgccctca aagccggcgg acactttcgc atggggccgc tcgaactgac cgattttatc 720

ggtcaggaca tcaactatca ggtcagccgg caaatttggc aggacatgca gtacgatccg 780

cgctataccc cgggccatct gcagcgctcg ctggtcgatg ccggtctgtt gggcaaaaag 840

aacggccgct cctatttttc cgccgaagaa tctcctccgc cgcttgcggc cgccgtcgat 900

gcggaggtcg agacgctacg catttacggt gaacatcctc tctttactct gctacagcag 960

cgggccaccc tgcaatggcc gcggctgcgc gtggaacaac ggccgacatt accgggcctg 1020

ggcgccgcca ttcaggttaa tgacgctttc accgtcagcg ttaccgatgg ccgcacggca 1080

aaccagcttg ccgaacagac cgcggcggac gcctttgtcg tcgatgtcgc cctgaactac 1140

ggcgatacgg cgtacctggt ggcggcgcat agccgccacg cctctgcagc caataaggcg 1200

ctgtttttac gcctgctgca caccgcgatc ccgcaggtgg aatttatcaa ggattccccg 1260

gccttgatcg tcgcccgcgt gctcagcagc ctgatcaacg agtcggtgat catggtggaa 1320

agcggcgtct gcagccggga agacattgat gtcgccgccg tcgccggcgt gaactacgcc 1380

gacggtattt tcggctggct cactcgcctc ggggaggaaa atgtcaggac gacgctgagc 1440

aacctggcgc aattgctgca cgcggcgcgc tatgcgccgc attacaccct tctgcacgcc 1500

gcccaaccgg cgctgaccac cacgccttaa 1530

<210> 92

<211> 1530

<212> DNA

<213> Serratia sp. BW106

<400> 92

atggcagaga ataattcggc aatccattcg gtcgctgtta ttggtgccgg aaccatgggc 60

agaggtattg cctatcttct ggcgcagaac ggcatacgaa ccctgcttta taatcgtagc 120

ggtaataatc tggatcaggc ccgcgactat attatccgtg acctggataa gaaaatagac 180

aacgggaaaa taagccaaca gaaaaaaggc gaagtattgg ccaacctggt tttctctccc 240

attttcgacg ctatcgccga cagcgacctg gtgattgaaa ccatcgcgga gcatgagacc 300

accaagcatg aaatcctcgc ggcgattgcg gccacggtga aacaagaggc cattatcgcc 360

accaacacct catcgctgtc gttgaataag ctggcggcag gcgtcgaaaa caacgcccgt 420

tttatcggcc tgcacttctt caatccggcc ccgctgatga aactgatcga gattattccg 480

tcctatttta ccagccgggc caccagcctg cgctgccagc agttggtgac ggcgctaggc 540

aaacagtttg tggtctgcaa agccacgccg ggttttatcg tcaaccggat ggcacggcct 600

ttttatctgg aaggattccg gctgttggaa gaaaacgtag cattggcacc gcagatcgac 660

cgcgccctca aggccggtgg ccactttcgc atgggccctt tagagctgac cgactttatc 720

ggccaggata ttaactatca ggtcagcagc cagatttggc aggacatgca gtacgaccct 780

cgctataccc ccggccattt gcaacgttcg ctggtggatg ccgggttgtt ggggaagaaa 840

aacggccgat ccttttttgc tgccccttct tccgaatcga accccctcga cgcaggcaac 900

ggcacgctga cttccctgca tttttatggc gaacataccc tgtttgacct gctgcaacag 960

cgcgccttgg ctatctggcc aacgctgcag attattcacc agccggaacg gccgacgctg 1020

ggacgcttta tccgggtgaa tgacgcattg gccgtcaaaa tcaccgatgg tcgcaccgcc 1080

aatctgctcg ctgaattgac cgatctcgac acctttgtga tcgacgccgc actgaattac 1140

agcgataccg cctatctggt ggccgcccac aatcaggacg ccgccgaggc caataaagcg 1200

ctgtttctgt cgctgctgca aacgttgatc ccgcaggtgg agtttattaa agactctcca 1260

ggcctgatcg tcgcccgggt tctgagcagt ctgatcaatg agtcggtgat catggtggag 1320

agcggggttt gcagccgggc agatatcgat attgccgccg tggcgggcgt taactatgcc 1380

gatggcatct ttgcctggct gacgcagctc gggcaaaaaa acgtgaaatc aacgctggat 1440

aatatggcgc aactgctgca ttccgcccgc tattacccgc attactcatt gctgaatgcc 1500

ccccggcccg aactggccgt cgcgccgtaa 1530

<210> 93

<211> 1530

<212> DNA

<213> Serratia liquefaciens FK01

<400> 93

atggcagaga ataatacggc aatagattcg gtcgccgtga ttggcgccgg aaccatgggc 60

agaggcattg cctatctttt ggcactgaac ggcatacgaa ccctgcttta taatcgaaac 120

ggtaatcatc ttaaccaggc ccgggattat attgtcggcg acctggataa aaaaatcgac 180

aacggaaaaa taagccaaca gaaaaagggc gaggtcctgg ccaatctggt tttctcgcca 240

gttttcgacg ccattgccga cagtgacctg gtgattgaaa ccatagcgga acatgaaccc 300

accaagcatg agatcctcgc ggccattgcc gccacggtaa aaaaagaggc gattatcgcc 360

accaatacct cttcgctgtc gctgaataaa ctggccgcag ggatagaaaa caactcgcgc 420

tttatcggcc tgcatttctt caacccggca ccgctgatga agctgatcga aattatcccg 480

tcctatttta ccgcccgggc caccacctta cgctgccagc agttggtcac ggcgataggc 540

aaaaaatttg tggtctgcaa agccacgccg ggctttatcg tcaatcgcat ggcgcggcct 600

ttttatctgg aaggttttcg cctgctggag gaaaacgtgg cgctggcacc gcaaatcgac 660

cgcgcgctca aggccggagg ccacttccgc atggggcctt tggaactgac tgattttatt 720

ggtcaggaca ttaattatca ggtcagcagc cagatttggc aggacatgca gtatgacccc 780

cgctataccc ccggccattt gcaacgttcg ctggtggatg ccgggctgtt ggggaagaaa 840

aacgggcgct cttttttttc cccctcggct gacgccgcca acccaccggc taccggcggc 900

ggtacgctga gctcgctgca tttttttggc gaacatccgc tgtttgatct gctgcaacag 960

cacgccttcg caacctgggc gcccctggca ataacacgtc agccggaaca cccggttctg 1020

ggccgtttta tccaggtgaa tgacagtctg gcagtcaaaa tcaccgacgg gcgaacggcc 1080

aatcagcttg ccgagctggc gggtctcgat accttcgtgg tcgacgttgc actgaactat 1140

gccaacaccg cttttctggt ggcggcccac agccaacagg ctaccgaggc gaataaagag 1200

ctgttcctca cgctgctgca aacggtgatc ccgcaggtgg agtttgttaa agattcccca 1260

ggcctgatcg tcgcccgggt tctgagcagc ctgatcaatg agtcggtgat catggtggag 1320

agcggggttt gtagccgaga agacatcgat atcgccgccg tggccggcgt caactatgcc 1380

gacggcattt ttgcctggct ggcgcagctc gggcagaaaa acgtgaaatc gacgctggat 1440

aatatggcgc aactgctgca ttccgcccgc tattacccgc actactcgtt gctccacgct 1500

accaggcccg agctggccgt cgcgccatga 1530

<210> 94

<211> 1530

<212> DNA

<213> Serratia sp. S119

<400> 94

atggcagaaa gtaatgcggc aattcaatcg gctgcgatta tcggcgcggg aacgatgggc 60

agaggcatcg cttatctttt cgcgcaaaaa ggcattcgca cggtgcttta taatcgcaac 120

ggcaataccc tcaatcaggc tcgcgaatat atcgcgcaag acctgaacaa gaaagtcgaa 180

cagggcaaga tcgcgctgca ggataaaggc gcagtgctgg ctaatctggt gttcacctcg 240

gtgtttgaga ctatcgccga cagcgatcta gtgatagaaa ccatcgccga gcaagagcaa 300

accaaacttg aggtgctggc ggccatcgcc gcggcggtca agcccgacac gctgatcgcc 360

accaatacat cctcactgtc gcttaacaag ctggcaaccg cggtgacaca cagcgagcgg 420

tttatcggtt tgcacttttt caatcccgcg ccgctgatga aactgattga aatcatcccg 480

gcctacttta ccgcacgcgc caccaccgaa cgttgccgtc agctggtggc cgcgttgggg 540

aaacgcgatg tcgtctgcca ggccacgccg gggtttatcg tcaaccgcat ggcccgcccc 600

tactacctgg aagggttccg cctgttggaa gaacacgtgg cgcgcgcgcc gcagatcgac 660

cgcgccctca aggccggcgg gcactttcgc atggggccgc tcgagctgac cgattttatc 720

ggccaggaca tcaactatca ggtcagccgg caaatctggc aggacatgca atacgatccg 780

cgctataccc ccggccatct gcagcgttcg ctggtcgatg ccggtctgtt ggggaaaaag 840

aacggccgct cctattttgc cgccgaagaa aacgccttac cggtaacggc cgccaccgat 900

gcagacgtcg agacgctgcg cgtttacggc gaacaccctt tttttaccct gttgcaacag 960

cgggccgcgc ttcagtggcc acggctgcgc gtggagcaac ggccggcctt accgggcctg 1020

gggccggccg tccagatcaa tgaggctttc accgtcagcg tcaccgatgg ccgcacggcg 1080

agccagttgg cggagctgac ggcggcggat gcctttgtgg tcgatgtcgc cctgaactac 1140

gccgacacgg cgtatctggt ggcagcgcac aaccgccacg cctctgcggc caataaggcg 1200

ctgtttttac gcctgctgca cacggcaatc ccgcaggttg aatttatcaa ggactccccg 1260

gcgctgatcg tcgcccgcgt cctcagcagc ctgatcaacg agtcggtgat catggtggaa 1320

agcggcgtct gcagccggga agacatcaat gtcgccgccg tcgccggcgt taactacgcc 1380

gacggcattt tcggcttgct cgatagcctg ggggagaaaa acgtcaggac gacgctgagc 1440

aacctggcgc agctgctgca cgcggcacgc tatgcgccgc attacaccct tctacacgcc 1500

gcgcaaccgg cgctgacgac cacgccttaa 1530

<210> 95

<211> 1530

<212> DNA

<213> Serratia sp. YD25

<400> 95

atggcagaac gtaatgcggc aattcaatcg gctgcgatta tcggcgcggg aacgatgggc 60

agaggcatcg cttatctttt cgcgcaaaaa ggcattcgta cggtgcttta taatcgcaac 120

ggcaataccc tcattcaggc tcgtgaatat atcgtgcaag acctggacaa aaaggtcgaa 180

cagggcaggc tcgcgccgca ggataaagac gcggtgctgg ccaatctgca gttctcatcg 240

gtgtttgaag ctatcgtcga cagcgatttg gtgctcgaaa ctatcgccga gcaagagcaa 300

gccaaactcg aggtgctggc cgccatcgct gcggcggtca aacccgacac gctgatcgcc 360

accaatactt cctcattgtc gctcaataag ctggcaacgg cggtgaccca cagcgaacgc 420

tttatcggtt tgcacttttt caatcccgca ccgctaatga agctgattga aatcattccg 480

gcctacttta ccacccaagc caccactgaa cgttgccgcc aactggtggc ggcattgggg 540

aaacgcgatg tcgtctgcca ggctacgccg gggttcatcg tcaaccgtat ggcccgcccc 600

tactacctgg aaggctttcg cctgttggaa gagcacgtgg cgcgcgcgcc gcagatcgac 660

cgcgccctca aggccggcgg acactttcgc atggggccgc tcgagctgac cgattttatc 720

ggccaggaca tcaactatca ggtcagccgg caaatttggc aggacatgca gtacgacccg 780

cgctataccc ccggccatct gcagcgttcg ctggtcgatg ccgggctgct ggggaaaaag 840

aacggtcgct cctatttttc cgccgaagaa accgccccgc cggttgaggc cgccgccgag 900

gcggatgtcg agacgctgcg catttacggc gaacaccctt tgtttaccct gctgcaacag 960

cgagccgcac tgcaatggcc gcagttgcgc gtggaacagc ggccggcctt gtcgggcctg 1020

ggagcggcca ttcaggtcaa tgacgctttc accgtcagcg tcaccgatgg ccgcacggca 1080

aatcagttgg ccgagcagac ggcggcggac gcctttgtgg tcgatgtcgc cctgaactac 1140

gccgacacgg cgtatctggt ggcggcgcac agccgccacg cctctgcggc taataaggcg 1200

ttgtttttgc gcctgctgca caccgcgctt ccgcaggttg aatttatcaa ggactccccg 1260

gcgctgatcg tcgcccgcgt cctcagcagc ctgatcaacg agtcggtgat catggtggaa 1320

agcggcgtct gcagccggga agacatcgat gtcgccgccg tcgccggcgt gaactacgcc 1380

gacggcattt tcggctggct cactcgcctg ggggagaaaa acgtcaggac gacgctgagc 1440

aacctggcgc agctgctgca cgcggcccgc tatgcgccgc attacaccct tctgcacgcc 1500

gcccaaccgg cgctgaccac cacgccttaa 1530

<210> 96

<211> 1530

<212> DNA

<213> Serratia sp. FS14

<400> 96

atggcagaaa gtaatgcggc aattcaatcg gctgcgatta tcggcgcggg aacgatgggc 60

agaggcatcg cttatctttt cgcgcaaaaa ggcattccca cgatgcttta taatcgcaac 120

ggcaataccc tcaatcaagc tcgcgaatat atcgcgcaag acctgaacaa gaaagtcgaa 180

cagggcaaga tcgcgttgca ggataaagac gcggtgctgg ccaatctgat gttcacttcc 240

gtgtttgagg ccatcgccga cagcgagctg gtgatagaaa ccatcgccga gcaagaacaa 300

accaaacttg aggtgctggc ggccatcgcc gcggtggtca agcccgacac gctgatcgcc 360

accaatacct cctcactgtc gcttaacaag ctggcaactg cggtgacgca cagcgagcgc 420

tttatcggtt tacatttttt caaccccgct ccgctgatga agctgattga aatcattccg 480

gcctacttta ccgcgcacgc caccacggaa cgctgccgcc aactggtggc ggcgttgggg 540

aaacgcgatg tcgtctgcca ggccacgccg gggttcatcg tcaatcgcat ggcccgcccc 600

tactacctgg aagggttccg cctgttggaa gaacatgtgg cgcgcgcggc gcagatcgac 660

cgcgccctca aggccggcgg gcgcttccgc atggggccgc tcgagctgac cgattttatc 720

ggccaagaca ttaactatca ggtcagccgg caaatctggc aggacatgca atacgacccg 780

cgctataccc ctggtcatct gcagcgttca ctggtcgatg ccggtctgtt ggggaaaaag 840

aacggccgct cctattttgc cgccgaagaa accgccccgc cggtgacggc cgccaacaat 900

gcagacgtcg agacgctgcg cgtttacggc gagcatcctt tttttaccct gttgcagcag 960

cgagccgcgc ttcagtggcc acagctgcgc gtggaacaac ggccggcatt accggggctg 1020

ggagcggccg tccagatcaa tgacgctttc accgtcagca tcaccgatgg ccgcacggcg 1080

agccaactgg ccgagcagac ggcagcggat gcctttgtgg tcgatgtcgc cctgaactac 1140

gccgacacca cgtacctggt ggcggcgcac agtcgccacg cttctgcggc caataaggcg 1200

ctgtttttac gcctgctgca cacggcaatc ccacaggttg aatttatcaa ggactctccg 1260

gcgcttatcg tcgcccgcgt cctcagcagc ctgatcaatg agtcggtgat catggtggaa 1320

agcggcgtct gcagccggga agacatcgat gtcgccgccg tcgcgggcgt taactacgcc 1380

ggcggcattt tcgactggct cggcaaactg ggggagaaaa acgtcaggac gacgctgagc 1440

aatctggcac agctgctgca cgcggcgcgc tatgcgccgc attacaccct tctgcacgcc 1500

gcgcaaccgg cgctgacgac cacgccttaa 1530

<210> 97

<211> 1530

<212> DNA

<213> Serratia sp. HMSC15F11

<400> 97

atggcagaaa gtaatgcggc aattcagtcg gctgcgatta tcggcgcggg aacgatgggc 60

agaggcatcg cttatctttt cgcgcaaaaa ggcattcgca cggtgcttta taatcgcaac 120

ggcaataccc tcaatcaggc tcgcgactat atcgagctag acctgaacaa gaaagtcgaa 180

cagggtaaga tcgcgctgca ggataaaggc gcggtgttgg ccaatctggt gttcacctcg 240

gtatttgaga ccattgccga cagcgagctg gtgatagaaa ccatcgccga gcaagagcaa 300

accaaacttg aggtgctggc ggccatcgcc gcggcagtca agcccgacac gctgatcgcc 360

accaatacct cctcactgtc gctgaataag ctggcaaccg cggtgacgca cagcgagcgg 420

tttatcggtt tgcacttttt taaccccgcg ccgctgatga aactgattga aatcatcccg 480

gcctacttta ccgcacacgc caccacggag cgttgccgtc aactggtggc cgcgttgggg 540

aaacgcgatg ttgtctgcca ggccacgccg gggtttatcg tcaaccgcat ggcccgcccc 600

tactacctgg aagggttccg cctattggaa gaacacgtgg cgcgcgcgcc gcagatcgac 660

cgtgccctca aggccggcgg gcactttcgt atggggccgc tcgagctgac cgattttatc 720

ggccaggaca tcaactatca ggtcagtcgg caaatctggc aggacatgca atacgacgcg 780

cgctataccc ccggccacct gcagcgttcg ctggtcgatg ccggtctatt gggaaaaaag 840

aacggccgct cctattttgc cgtcgaagaa aacgccccgc cggtgatggc cgccaccgat 900

gcagacattg agacgctgca cgtttacggc gaacaccctt tttttaccct gttacaacaa 960

cgtgccgcgc ttcagtggcc acagctgcgc gtggaacaac ggccggcatt accgggcctg 1020

gggccggccg tccggatcaa tgacgctttc accgtcagca tcaccgatgg ccgcacggca 1080

aaccagctgg ccgagcagac ggcggcggat gcctttgtgg tcgatgtcgc cctgaattac 1140

gccgacacgg cgtatctggt ggcagcgcac agccgccacg cctctgcagc caataaagcg 1200

ctgttcttac gcctgctgca cacggcaatc ccgcaggttg aatttatcaa ggattccccg 1260

gcgctgatcg tcgcccgcgt cctcagcagc ctaatcaacg agtcggtgat catggtggaa 1320

agcggcgtct gcagccggga agacatcgat gtcgccgccg tcgccggcgt taactacgcc 1380

gacggcattt tcggctggct agatagcctg ggggagaaaa acgtcaggac gacgctgagc 1440

aacctggcgc agctgctgca cgcagcacgc tatgcgccgc attacaccct tctgcacgcc 1500

gcgcaaccgg cgctgacgac cacgccttaa 1530

<210> 98

<211> 1530

<212> DNA

<213> Serratia sp. JKS000199

<400> 98

atggcagaaa gtaatgcggc aattcaatcg gctgcgatta tcggcgcggg aacgatgggc 60

agaggcatcg cttatctttt ggcgcaaaaa agcattcgta cggtgcttta taatcgcaac 120

ggcaataccc tcaatcaagc tcgcgacgct atcgtgcaag acctgaacaa aaaggtggaa 180

cagggcaagc tcgcgctgca ggacaaagac gcggtgctgg ctaatctgac gttctccacg 240

gaatttgggg ccatcgccga cagcgatctg gtgatcgaaa ccatcgcaga gcatgagcaa 300

gccaaactcg aagtgctggc ggccatcgcc gcgacggtca agcccgacac gctgatcgcc 360

accaatacct cctcactgtc gcttaataag ctggcgacgg cggtgacgca cagcgaacgc 420

tttatcggtt tgcacttttt caaccccgcg ccgctgatga agctgattga aatcattccg 480

gcctatttta ccgcacaggt caccaccgaa cgttgccgtc aactggtggc ggcgttaggg 540

aaacgcgatg tcgtctgtca ggccacgccg ggatttatcg tcaaccgtat ggcccgcccc 600

tactatctgg aagggttccg cctgttggaa gaacacgtgg cgcgtgcgcc gcagatcgac 660

cgcgccctca aagccggcgg acactttcgc atggggccgc tcgagctgac cgattttatc 720

ggtcaggaca tcaactatca ggtcagccgg caaatttggc aggacatgca gtacgatccg 780

cgctataccc cgggccatct gcagcgctcg ctggtcgatg ccggtctgtt gggcaaaaag 840

aacggccgct cctatttttc cgccgaagaa tctcccccgc cgcttgcggc cgccgtcgat 900

gcggaagtcg agacgctacg catttacggt gaacatcctc tctttaccct gctacagcag 960

cgggccgccc tgcaatggcc gcggctgcgc gtggaacaac ggccgacatt accgggcctg 1020

ggcgccgcca ttcaggtcaa tgacgctttc accgtcagcg ttaccgatgg ccgcacggca 1080

aaccagcttg ccgaacagac cgcggcggac gcctttgtcg tcgatgtcgc cctgaactac 1140

ggcgatacgg cgtacctggt ggcggcgcat agccgccacg cctctgcggc caataaggcg 1200

ctgtttttgc gcctgctgca caccgcgatc ccgctggtgg aatttatcaa ggattccccg 1260

gccttgatcg tcgcccgcgt gctcagcagc ctgatcaacg agtcggtgat catggtggaa 1320

agcggcgtct gcagccggga agacattgat gtcgccgccg tcgccggcgt gaactacgcc 1380

gacggtattt tcggctggct cactcgcctc ggggaggaaa atgtcaggac gacgctgagc 1440

aacctggcgc aattgctgca cgcggcgcgc tatgcgccgc attacaccct tctgcacgcc 1500

gcccaaccgg cgctgaccac cacgccttaa 1530

<210> 99

<211> 1530

<212> DNA

<213> Serratia sp. TEL

<400> 99

atggcagaaa gtaatgcggc aattcaatcg gctgcgatta tcggcgcggg aacgatgggc 60

agaggcatcg cttatctttt ggcgcaaaaa agcattcgta cggtgcttta taatcgcaac 120

ggcaataccc tcaatcaagc tcgcgacgct atcgtgcaag acctgaacaa aaaggtggaa 180

cagggcaagc tcgcgctgca ggacaaagac gcggtgctgg ctaatctgac gttctccgcg 240

gaatttgggg ccatcgccga cagcgatctg gtgatcgaaa ccatcgcaga gcatgagcaa 300

gccaaactcg aggtgctggc ggccatcgcc gcgacggtca agcccgacac gctgatcgcc 360

accaatacct cctcactgtc gcttaataag ctggcgacgg cggtgacgca cagcgaacgc 420

tttatcggtt tgcacttttt caaccccgcg ccgctgatga agctgattga aatcattccg 480

gcctatttta ccgcacaggt caccaccgaa cgttgccgtc aactggtggc ggcgttgggg 540

aaacgcgatg tcgtctgtca ggccacgccg ggatttatcg tcaaccgtat ggcccgcccc 600

tactatctgg aagggttccg cctgttggaa gaacacgtgg cgcgtgcgcc gcagatcgac 660

cgcgccctca aagccggcgg acactttcgc atggggccgc tcgaactgac cgattttatc 720

ggtcaggaca tcaactatca ggtcagccgg caaatttggc aggacatgca gtacgatccg 780

cgctataccc cgggccatct gcagcgctcg ctggtcgatg ccggtctgtt gggcaaaaag 840

aacggccgct cctatttttc cgccgaagaa tctcctccgc cgcttgcggc cgccgtcgat 900

gcggaggtcg agacgctacg catttacggt gaacatcctc tctttactct gctacagcag 960

cgggccaccc tgcaatggcc gcggctgcgc gtggaacaac ggccgacatt accgggcctg 1020

ggcgccgcca ttcaggttaa tgacgctttc accgtcagcg ttaccgatgg ccgcacggca 1080

aaccagcttg ccgaacagac cgcggcggac gcctttgtcg tcgatgtcgc cctgaactac 1140

ggcgatacgg cgtacctggt ggcggcgcat agccgccacg cctctgcagc caataaggcg 1200

ctgtttttac gcctgctgca caccgcgatc ccgcaggtgg aatttatcaa ggattccccg 1260

gccttgatcg tcgcccgcgt gctcagcagc ctgatcaacg agtcggtgat catggtggaa 1320

agcggcgtct gcagccggga agacattgat gtcgccgccg tcgccggcgt gaactacgcc 1380

gacggtattt tcggctggct cactcgcctc ggggaggaaa atgtcagggc gacgctgagc 1440

aacctggcgc aattgctgca cgcggcgcgc tatgcgccgc attacaccct tctgcacgcc 1500

gcccaaccgg cgctgaccac cacgccttaa 1530

<210> 100

<211> 1530

<212> DNA

<213> Serratia sp. ISTD04

<400> 100

atggcagaaa gtaatgcggc aattcaatcg gctgcgatta tcggcgcggg aacgatgggc 60

agaggcatcg cttatctttt ggcgcaaaaa agcattcgta cggtgcttta taatcgcaac 120

ggcaataccc tcaatcaagc tcgcgacgct atcgtgcaag acctgaacaa aaaggtggaa 180

cagggcaagc tcgcgctgca ggacaaagac gcggtgctgg ctaatctgac gttctccgca 240

gaatttgggg tcatcgccga cagcgatctg gtgatcgaaa ccatcgcaga acatgagcaa 300

gccaaactcg aggtgctggc ggccatcgcc gcgacggtca agtccgacac gctgatcgcc 360

accaatacct cctcactgtc gcttaataag ctggcgacgg cggtgacgca cagcgaacgc 420

tttatcggtt tgcacttttt caaccccgcg ccgctgatga agctgattga aatcattccg 480

gcctatttta ccgcacaggt caccaccgaa cgttgccgtc aactggtggc ggcgttaggg 540

aaacgcgatg tcgtctgtca ggccacgccg ggatttatcg tcaaccgtat ggcccgcccc 600

tactatctgg aagggttccg cctgttggaa gaacacgtgg cgcgtgcgcc gcagatcgac 660

cgcgccctca aagccggcgg acactttcgc atggggccgc tcgagctgac cgattttatc 720

ggtcaggaca tcaactatca ggtcagccgg caaatttggc aggacatgca gtacgatccg 780

cgctataccc cgggccatct gcagcgctcg ctggtcgatg ccggtctgtt gggcaaaaag 840

aacggccgct cctatttttc cgccgaagaa tctcccccgc cgcttgcggc cgccgtcgat 900

gcggaagtcg agacgctacg catttacggt gaacatcctc tctttactct gctacagcag 960

cgggccgccc tgcaatggcc gcggctgcgc gtggaacaac ggccgacatt accgggcctg 1020

ggcgccgcca ttcaggtcaa tgacgctttc accgtcagcg ttaccgatgg ccgcacggca 1080

aaccagcttg ccgaacagac cgcggcggac gcctttgtcg tcgatgtcgc cctgaactac 1140

ggcgatacgg cgtacctggt ggcggcacat agccgccatg cctctgcggc caataaggcg 1200

ctgtttttac gcctgctgca caccgcgatc ccgcaggtgg aatttatcaa ggattccccg 1260

gccttgatcg tcgcccgcgt gctcagcagc ctgatcaacg agtcggtgat catggtggaa 1320

agcggcgtct gcagccggga agacattgat gtcgccgccg tcgccggcgt gaactacgcc 1380

gacggtattt tcggctggct cactcgcctc ggggaggaaa atgtcaggac gacgctgagc 1440

aacctggcgc aattgctgca cgcggcgcgc tatgcgccgc attacaccct tctgcacgcc 1500

gcccaaccgg cgctgaccac cacgccttaa 1530

<210> 101

<211> 1530

<212> DNA

<213> Serratia sp. SCBI

<400> 101

atggcagaaa gtaatgcggc aattcaatcg gctgcgatta tcggcgcggg aacgatgggc 60

agaggcatcg cttatctttt ggcgcaaaaa agcattcgta cggtgcttta taatcgcaac 120

ggcaataccc tcaatcaagc tcgcgacgct atcgtgcaag acctgaacaa aaaggtggaa 180

cagggcaagc tcgcgctgca ggacaaagac gcggtgctgg ctaatctgac gttctccgcg 240

gaatttgggg ccatcgccga cagcgatctg gtgatcgaaa ccatcgcaga gcatgagcaa 300

gccaaactcg aggtgctggc ggccatcgcc gcgacggtca agcccgacac gctgatcgcc 360

accaatacct cctcactgtc gcttaataag ctggcgacgg cggtgacgca cagcgaacgc 420

tttatcggtt tgcacttttt caaccccgcg ccgctgatga agctgattga aatcattccg 480

gcctatttta ccgcacaggt taccaccgaa cgttgccgtc aactggtggc ggcgttgggg 540

aaacgcgatg tcgtctgtca ggccacgccg ggatttatcg tcaaccgtat ggcccgcccc 600

tactatctgg aagggttccg cctgttggaa gaacacgtgg cgcgtgcgcc gcagatcgac 660

cgcgccctca aagccggcgg acactttcgc atggggccgc tcgagctgac cgattttatc 720

ggtcaggaca tcaactatca ggtcagccgg caaatttggc aggacatgca gtacgatccg 780

cgctataccc cgggccatct gcagcgctcg ctggtcgatg ccggtctgtt gggcaaaaag 840

aacggccgct cctatttttc caccgaagaa tctcccccgc cgcttgcggc cgccgtcgat 900

gcggaggtcg agacgctacg catttacggt gaacatcctc tctttaccct gctacagcag 960

cgggccgccc tgcaatggcc gcggctgcgc gtggaacaac ggccgacatt accgggcctg 1020

ggcgccgcca ttcaggtcaa tgacgctttc accgtcagcg ttaccgatgg ccgcacggca 1080

aaccagcttg ccgaacagac cgcggcggac gcctttgtcg tcgatgtcgc cctgaactac 1140

ggcgatacgg cgtacctggt ggcggcgcat agccgccacg cctctgcggc caataaggcg 1200

ctgtttttac gcctgctgca caccgcgatc ccgcaggtgg aatttatcaa ggattccccg 1260

gccttgatcg tcgcccgcgt gctcagcagc ctgatcaacg agtcggtgat catggtggaa 1320

agcggcgtct gcagccggga agacattgat gtcgccgccg tcgccggcgt gaactacgcc 1380

gacggcattt tcggctggct cactcgcctc ggggaggaaa atgtcaggac gacgctgagc 1440

aacctggcgc aattgctgca cgcggcgcgc tatgcgccgc attacaccct tctgcacgcc 1500

gcccaaccgg cgctgaccac cacgccttaa 1530

<210> 102

<211> 1530

<212> DNA

<213> Serratia sp. S4

<400> 102

atggcagaga ataattcggc aatccattcg gtcgctgtta ttggtgccgg gaccatgggc 60

agaggtattg cctatcttct ggcgcaaaac ggcatacgaa ccctgcttta taatcgtagc 120

ggtaataatc tgaatcaggc cagggactat attatccgtg acctggataa gaaaatagat 180

ggcggtaaaa taagcctgca gaagaaaggc gagatattgg ccaatctggt tttctctcct 240

attttcgagg ctattgccga cagcgacctg gtgattgaaa ccatcgcgga acatgagtca 300

accaagcatg agatcctctc ggcgattgcg gccacggtga aaaaagaggc gattatcgcc 360

accaatacct catcgctgtc attgaataag ctggcggcag gcgtcgaaaa taacgcccgt 420

tttatcggcc tgcacttctt caatccggcc ccgctgatga aactgatcga aattattccg 480

tcttacttta ccagccgggc caccagcctg cgttgccagc agttggtgac ggtgataggc 540

aaacagtttg tggtctgcaa agccacgccg ggctttatcg tcaaccggat ggcgcgacct 600

ttttatctgg aagggtttcg gctgttggaa gagaacgtgg cgctggcgcc gcagatcgac 660

cgcgcactca aggccggtgg ccacttccgc atgggacctt tagagctgac cgattttatc 720

ggccaggata ttaactatca ggtcagcagc cagatttggc aggacatgca gtacgacccc 780

cgctataccc ccggtcattt gcaacgttcg ctggtggatg ccgggctgct ggggaagaaa 840

aacggccgat ccttttttac cccctcttcc gccacaccca gctccgccga cgcaggcagc 900

ggcacgccga cctcactgaa tttttatggt gaacatcccc tgttcgacct gttgcaacag 960

cgcgctttgg cgctctggcc aaggttgcag attaatcgcc aatcggaaca gccaacgctg 1020

ggccgcttta tccgggtgaa tgacgcaatg gccatcaaaa ttaccgatgg ccgcaccgcc 1080

aatctgctgg ctgaattgac cgaactcgat accttcgtga tcgacgccgc gctcaactac 1140

gccgataccg cctatctggc ggctgcccac agccaggatg ccagcacggc caataaagcg 1200

ctgtttctga cgctgctgca aacgttgatc ccacaggtgg agtttattaa agactccccg 1260

ggcctgattg tcgcccgcgt cctgagcagc ctgatcaatg agtcggtgat tatggtggag 1320

agcggggttt gcagccgggc ggacatcgat atcgccgcgg tggccggcgt taactatgcc 1380

gatggcatct ttagctggct ggcgcagctt gggcaaaaaa acgtgaagtc gacgctggac 1440

aatatggctc aactgctgca ttccgcccgc tattacccgc attactcttt gctcaatacc 1500

ccccggccag agctggccgt cgcgccctaa 1530

<210> 103

<211> 1530

<212> DNA

<213> Serratia sp. C-1

<400> 103

atggcagaga ataattcggc aatccgttca gccgccgtta ttggtgcggg gaccatgggc 60

agaggcatcg cctacctcct ggcactgaac ggcatacgaa ccgtacttta taatcgcaat 120

ggtaataatc tcaatcaggc ccgtgactat attgtcagcg acctggacag aaaaatagat 180

aacggaaaaa taaccctgca gaaaaaaggc cagatattag ccaatattat tttctcggcc 240

gtctttgacg ccataaccga cagcgatctg gtgattgaaa ccattgcgga agatgagcaa 300

accaagcatg aaatcctggc agccattgcg gctacggtaa aaccggaggc gatcattgcg 360

accaatacct cctcgttgtc gctgaacaaa ctggcagcag gggtggaaaa caacccgcgc 420

tttatcggcc tgcatttttt caatccggcg ccgctgatga agttgatcga aatcattccc 480

tcttatttta cctcccacgc caccagccta cgctgccaga agttggtaat agcattgggt 540

aaacagtttg tggtctgcaa agccacgccg ggctttattg ttaatcgcat ggcgcggcct 600

ttctatctgg aagggttccg gctgctggag gaaaacgtgg cgctggcgcc acagatcgac 660

cgcgccctca aggccggcgg gcattttcgc atgggccctt tagaactgac ggattttatc 720

ggtcaggata tcaactacca ggtcagcaag cagatttggc aggatatgca gttcgaccct 780

cgctataccc ctggtcattt gcaacgctcg ctggtggatg ccgggctgct ggggaggaaa 840

aacgggcgct ctttttttgc ttcccaaccg gcgacgccgc ccaccccgac cacagagagc 900

gacacgccaa cgtcactgca tttttatggg gagcatgctt tattcgatca tctgcaacag 960

cgtgctctgg ccgcctggcc tgcgctgcgc gttcagcggt tgccggaacg gcctgaactg 1020

gggcgattta tcctggtgaa taacgcgctg gcgatcaaaa tcaccgatgg cagaacggca 1080

aacctgctcg ccggcttaac cgctctcgac accttcgtga ttgacgctgc gctgaattac 1140

gccgacaccg cctatctggt ggcagcccac aatcaacatg ccacagagac gaataaagcg 1200

ctgtttctga cgctgctgca aaccgtcatc gctcaggtgg agtttattaa agattcccct 1260

gccctgatcg ttgcccgcgt actgagcagc ctgatcaatg aatcggtgat catggtggag 1320

agcggcgttt gcagccgggc agatatcgat atcgccgccg tggccggcgt gaactatgcc 1380

gacggcattt ttgcctggtt ggcgcagctc gggcagaaaa acgtgaaatc gacgctggat 1440

aacatggcgc aactgctgca ctccgcgcgc tattacccgc attactcttt gctgaacgcg 1500

gcccggcctg agctggccgt agcgccctaa 1530

<210> 104

<211> 1530

<212> DNA

<213> Serratia marcescens 532

<400> 104

atggcagaaa gtaatgcggc aattcaatcg gctgcgatta tcggcgcggg aacgatgggc 60

agaggcatcg cttatctttt cgcgcaaaaa ggcattcgca cggtgcttta taatcgcaac 120

ggcaataccc tcaatcaggc tcgcgaatat atcgcgcaag acctgaacaa gaaagtcgaa 180

cagggcaaga tcgcgctgca ggataaaggc gcggtgctgg ccaatctgat gttcacttca 240

gtgtttgagg ccatcaccga cagcgagctg gtgatagaaa ccatcgccga gcaagaacaa 300

accaaacttg aggtgctggc ggccatcgcc gcggtggtca agcccgacac gctgatcgcc 360

accaatacct cctcactgtc gcttaacaag ctggctactg cggtgacgca cagcgaacgc 420

tttatcggtt tgcatttttt caaccccgcg ccgctgatga agctgattga aatcattccg 480

gcctacttta ccgcgcacgc caccaccgaa cgctgccgcc aactggtggc ggcgttgggg 540

aaacacgatg tcgtctgcca ggccacgccg gggttcatcg tcaatcgcat ggcccgcccc 600

tactacctgg aagggttccg tctgttggaa gaacacgtgg cgcgcgcggc gcagatcgac 660

cgcgctctca aggccggcgg gcgcttccgc atggggccgc tcgagctgac cgattttatc 720

ggccaagaca tcaactatca ggtcagtcgg caaatctggc aggacatgca atacgacccg 780

cgctataccc ccggtcatct gcagcgttca ctggtcgatg ccggtctgtt ggggaaaaag 840

aacggccgct cctattttgc cgccgaagaa accgccccgc cggtgacggc cgccagcaat 900

gcagacgtcg agacgctgcg cgtttacggc gagcaccctt tttttaccct gttacagcag 960

cgagccgcgc ttcagtggcc acagctgcgc gtggaacaac ggccggcatt accggggctg 1020

gggtcggccg tccagatcaa tgacgctttc accgtcagca tcaccgatgg ccgcacggcg 1080

agccaactgg ccgagcagac ggcagcagat gcctttgtgg tcgatgtcgc cctgaactac 1140

gccgacacga cgtatctggc ggcggcgcac agccgccacg cctctgcggc caataaggcg 1200

ctgtttttac gcctgctgca cacggcaatc ccgcaggttg aatttatcaa ggactctccg 1260

gcgcttatcg tcgcccgcgt cctcagcagc ctgatcaatg agtcggtgat catggtggaa 1320

agcggcgtct gcagccggga agacatcgat gtcgccgccg tcgcgggcgt taactacgcc 1380

ggcggcattt tcgactggct cggcaaactg ggggagaaaa acgtcaggac aacactgagc 1440

aatctggctc agctgctgca cgcggcgcgc tatgcgccgc attacaccct tctgcacgcc 1500

gcgcaaccgg cgctgacgac cacgccttaa 1530

<210> 105

<211> 1530

<212> DNA

<213> Serratia marcescens 2880STDY5683033

<400> 105

atggcagaaa gtaatgcggc aattcaatcg gctgcgatta tcggcgcggg aacgatgggc 60

agaggcatcg cttatctttt cgcgcaaaaa ggcattcgca cggtgcttta taatcgcaac 120

ggcaataccc tcaatcaggc tcgcgaatat atcgcgcacg acctgaacaa gaaagtcgaa 180

caaggcaaga tcgcgctgca ggataaaggc gcggtgctgg ccaatctaat gttcacttca 240

gtgtttgagg ccatcgccga cagcgagctg gtgatagaaa ccatcgccga gcaagaacaa 300

accaaacttg aggtgctggc ggccatcgcc gcggtggtca agcccgacac gctgatcgcc 360

accaatacct cctcactgtc gcttaacaag ctggctactg cggtgacgca cagcgaacgc 420

tttatcggtt tgcatttttt caaccccgcg ccgctgatga agctgattga aatcattccg 480

gcctacttta ccgcgcacgc caccaccgaa cgctgccgcc aactggtagc ggcgttgggg 540

aaacacgatg tcgtctgcca ggccacgccg gggttcatcg tcaatcgcat ggcccgcccc 600

tactacctgg aagggttccg cctgttggaa gaacacgtgg cgcgcgcggc gcagatcgac 660

cgcgccctca aggccggcgg gcgcttccgc atggggccgc tcgagctgac cgattttatc 720

ggccaagaca tcaactatca ggtcagtcgg caaatctggc aggatatgca atacgacccg 780

cgctataccc ccggtcatct gcagcgttca ctggtcgatg ccggtctgtt ggggaaaaag 840

aacggccgct cctattttgc caccgaagaa accgccccgc cggtgacggc cgccagcaat 900

gcagacgtcg agacgctgcg cgtttacggc gagcaccctt tttttaccct gttacagcag 960

cgagccgcgc ttcagtggcc acagctgcgc gtggaacaac ggccggcatt accggggctg 1020

gggtcggccg tccagatcaa tgacgctttc accgtcagca tcaccgatgg ccgcacggcg 1080

agccaactgg ccgagcagac ggcagcggat gcctttgtgg tcgatgtcgc cctgaactac 1140

gccgacacga cgtatctggc ggcggcgcac agccgccacg cctctgcggc caataaggcg 1200

ctgtttttac gcctgctgca cacggcaatc ccgcaggttg aatttatcaa ggactctccg 1260

gcgcttatcg tcgcccgcgt cctcagcagc ctgatcaatg agtcggtgat catggtggaa 1320

agcggcgtct gcagccggga agacatcgat gtcgccgccg tcgcgggcgt taactacgcc 1380

ggcggcattt tcgactggct cggcaaactg ggggagaaaa acgtcaggac aacgctgagc 1440

aatctggctc agctgctgca cgcggcgcgc tatgcgccgc attacaccct tctgcacgcc 1500

gcgcaaccgg cgctgacgac cacgccttaa 1530

<210> 106

<211> 1530

<212> DNA

<213> Serratia marcescens WW4

<400> 106

atggcagaaa gtaatgcggc aattcaatcg gctgcgatta tcggcgcggg aacgatgggc 60

agaggcatcg cttatctttt cgcgcaaaaa ggcattccca cgatgcttta taatcgcaac 120

ggcaataccc tcaatcaagc tcgcgaatat atcgcgcaag acctgaacaa gaaagtcgaa 180

cagggcaaga tcgcgctgca ggataaagac gcggtgctgg ccaatctgat gttcacttcc 240

gtgtttgagg ccatcgccga cagcgagctg gtgatagaaa ccatcgccga gcaagaacaa 300

accaaacttg aggtgctggc ggccatcgcc gcagtggtca agcccgacac gctgatcgcc 360

accaatacct cctcactgtc gcttaacaag ctggcaactg cggtgacgca cagcgagcgc 420

tttatcggtt tgcatttttt caaccccgcg ccgctgatga agctgattga aatcattccg 480

gcctacttta ccgcacacgc caccacggaa cgctgccgcc aactggtggc ggcgttgggg 540

aaacgcgatg tcgtctgcca ggccacgccg gggttcatcg tcaatcgcat ggcccgcccc 600

tactacctgg aagggttccg cctgttggaa gaacatgtgg cgcgcgcggc gcagatcgac 660

cgcgccctca aggccggcgg gcgcttccgc atggggccgc tcgagctgac cgattttatc 720

ggccaagaca ttaactatca ggtcagccgg caaatctggc aggacatgca atacgacccg 780

cgctataccc ctggtcatct gcagcgttca ctggtcgatg ccggtctgtt ggggaaaaag 840

aacggccgct cctattttgc cgccgaagaa accgccccgc cggtgacggc cgccaacaat 900

gcagacgtcg agacgctgcg cgtttacggc gagcatcctt tttttaccct gttgcagcag 960

cgagccgcgc ttcagtggcc acagctgcgc gtggaacaac ggccggcatt accggggctg 1020

ggagcggccg tccagatcaa tgacgctttc accgtcagca tcaccgatgg ccgcacggcg 1080

agccaactgg ccgagcagac ggcagcggat gcctttgtga tcgatgtcgc cctgaactac 1140

gccgacacca cgtatctggt ggcggcgcac agccgccacg cttctgcggc caataaggcg 1200

ctgtttttac gcctgctgca cacggcaatc ccgcaggttg aatttatcaa ggactccccg 1260

gcgcttatcg tcgcccgcgt cctcagcagc ctgatcaatg agtcggtgat catggtggaa 1320

agcggcgtct gcagccggga agacatcgat gtcgccgccg tcgcgggcgt taactacgcc 1380

ggcggcattt tcgactggct cggcaaactg ggggagaaaa acgtcaggac gacgctgagc 1440

aatctggcgc agctgctgca cgcggcgcgc tatgcgccgc attacaccct tctgcacgcc 1500

gcgcaaccgg cgctgacgac cacgccttaa 1530

<210> 107

<211> 1530

<212> DNA

<213> Serratia marcescens K27

<400> 107

atggcagaaa gtaatgcggc aattcaatcg gctgcgatta tcggcgcggg aacgatgggc 60

agaggcatcg cttatctttt cgcgcaaaaa ggcattccca cgatgcttta taatcgcaac 120

ggcaataccc tcaatcaagc tcgcgaatat atcgcgcaag acctgaataa gaaagtcgaa 180

cagggcaaga tcgcgctgca ggataaagac gcggtgctgg ccaatctgat gttcacttcc 240

gtgtttgagg ccatcgccga cagcgagctg gtaatagaaa ccatcgccga gcaagaacaa 300

accaaacttg aggtgctggc ggccatcgcc gcggtggtca agcccgacac gctgatcgcc 360

accaatacct cctcactgtc gctcaacaag ctggcaactg cggtgacgca cagcgaacgc 420

tttatcggtt tacatttttt caaccccgcg ccgctgatga agctgattga aatcattccg 480

gcctacttta ccgcgcacgc caccacggaa cgctgccgcc aactggtggc ggcgttaggg 540

aaacgcgatg tcgtctgcca ggccacgccg gggttcatcg tcaatcgcat ggcccgcccc 600

tactacctgg aagggttccg cctgttggaa gaacatgtgg cgcgctcggc gcagatcgac 660

cgcgccctca aggccggcgg gcgcttccgc atggggccgc tcgagctgac cgattttatc 720

ggccaagaca ttaactatca ggtcagccgg caaatctggc aggacatgca atacgacccg 780

cgctataccc ctggtcatct gcagcgttca ctggtcgatg ccggtctgtt ggggaaaaag 840

aacggccgct cctattttgc cgccgaagaa accgccccgc cggtgacggc cgccaacaat 900

gcagacgtcg agacgctgcg cgtttacggc gagcatcctt tttttaccct gttgcagcag 960

cgagccgcgc ttcagtggcc acagctgcgc gtggaacaac ggccggcatt accggggctg 1020

ggagcggccg tccagatcaa tgacgctttc accgtcagca tcaccgatgg ccgcacggcg 1080

agccaactgg ccgagcagac ggcagcggat gcctttgtgg tcgatgtcgc cctgaactac 1140

gccgacacca cgtatctggt ggcggcgcac agccgccacg cttctgcggc caataaggcg 1200

ctgtttttac gcctgctgca cacggcaatc ccgcaggttg aatttatcaa ggactctccg 1260

gcgcttatcg tcgcccgcgt cctcagcagc ctgatcaatg agtcggtgat catggtggaa 1320

agcggcgtct gcagccggga agacatcgat gtcgccgccg tcgcgggcgt taactacgcc 1380

ggcggcattt tcgactggct cggcaaactg ggggagagaa acgtcaggac gacgctgagc 1440

aatctggcgc agctgctgca cgcggcgcgc tatgcgccgc attacaccct tctgcacgcc 1500

gcgcaaccgg cgctgacgac cacgccttaa 1530

<210> 108

<211> 1530

<212> DNA

<213> Serratia marcescens 280

<400> 108

atggcagaaa gtaatgcggc aattcaatcg gctgcgatta tcggcgcggg aacgatgggc 60

agaggcatcg cttatctttt cgcgcaaaaa ggcattccca cgatgcttta taatcgcaac 120

ggcaataccc tcaatcaagc tcgcgaatat atcgcgcaag acctgaataa gaaagtcgaa 180

cagggcaaga tcgcgctgca ggataaagac gcggtgctgg ccaatctgat gttcacttcc 240

gtgtttgagg ccatcgccga cagcgagctg gtaatagaaa ccatcgccga gcaagaacaa 300

accaaacttg aggtgctggc ggccatcgcc gcagtggtca agcccgacac gctgatcgcc 360

accaatacct cctcactgtc gcttaacaag ctggcaactg cggtaacgca cagcgaacgc 420

tttatcggtt tgcatttttt caaccccgcg ccgctgatga agctgattga aatcattccg 480

gcctacttta ccgcgcacgc caccacggaa cgctgccgcc aactggtggc ggcgttgggg 540

aaacgcgatg tcgtctgcca ggccacgccg gggttcatcg tcaatcgcat ggcccgcccc 600

tactacctgg aagggttccg cctgttggaa gaacatgtgg cgcgcgcggc gcagatcgac 660

cgcgccctca aggctggcgg gcgcttccgc atggggccgc tcgagctgac cgattttatc 720

ggccaagaca tcaactatca ggtcagccgg caaatctggc aggacatgca atacgacccg 780

cgctataccc ctggtcatct gcagcgttca ctggtcgatg ccggtctgtt ggggaaaaag 840

aacggccgct cctattttgc cgccgaagaa accgccccgc cggtgacggc cgccaacaat 900

gcagacgtcg agacgctgcg cgtttacggc gagcaccctt tttttaccct gttgcagcag 960

cgagccgcgc ttcagtggcc acagctgcgc gtggaacaac ggccggcatt accggggctg 1020

ggagcggccg tccagatcaa tgacgctttc accgtcagca tcaccgatgg ccgcacggcg 1080

agccaactgg ccgagcagac ggcagcggat gcctttgtgg tcgatgtcgc cctgaactac 1140

gccgacacca cgtatctggt ggcggcgcac agccgccacg cttctgctgc caataaggcg 1200

ctgtttttac tcctgctgca cacggcaatc ccgcaggttg aatttatcaa ggactccccg 1260

gcgcttatcg tcgcccgcgt cctcagcagc ctgatcaatg agtcggtgat catggtggaa 1320

agcggcgtct gcagccggga agacatcgat gtcgccgccg tcgcgggcgt taactacgcc 1380

ggcggcattt tcgactggct cggcaagctg ggggagaaaa acgtcaggac gacgctgagc 1440

aatctggcgc agctgctgca cgcggcgcgc tatgcgccgc attacaccct tctgcacgcc 1500

gcgcaaccgg cgctgacgac cacgccttaa 1530

<210> 109

<211> 1530

<212> DNA

<213> Serratia marcescens 19F

<400> 109

atggcagaaa gtaatgcggc aattcaatcg gctgcgatta tcggcgcggg aacgatgggc 60

agaggcatcg cttatctttt cgcgcaaaaa ggcattccca cgatgcttta taatcgcaac 120

ggcaataccc tcaatcaagc tcgcgaatat atcgcgcaag acctgaacaa gaaagtcgaa 180

cagggcaaga tcgcgctgca ggataaggac gcggtgctgg ccaatctgat gttcacttcc 240

gtgtttgagg ccatcgccga cagcgagctg gtaatagaaa ccatcgccga gcaagaacaa 300

accaaacttg aggtgctggc ggccatcgcc gcggtggtca agcacgacac gctgatcgcc 360

accaatacct cctcactgtc gcttaacaag ctggcaactg cggtgacgca cagcgaacgc 420

tttatcggtt tgcatttttt caaccccgcg ccgctgatga agctgattga aatcattccg 480

gcctacttta ccgcgcacgc caccacggaa cgctgccgcc aactggtggc ggcgttgggg 540

aaacgcgatg tcgtctgcca ggccacgccg gggttcatcg tcaatcgcat ggcccgcccc 600

tactacctgg aagggttccg cctgttggaa gaacatgtgg cgcgcgcggc gcagatcgac 660

cgcgccctca aggccggcgg gcgcttccgc atggggccgc tcgagctgac cgattttatc 720

ggccaagaca ttaactatca ggtcagccgg caaatctggc aggacatgca atacgacccg 780

cgctataccc ctggtcatct gcagcgttca ctggtcgatg ccggtctgtt ggggaaaaag 840

aacggccgct cctattttgc cgccgaagaa accgccccgc cggtgacggc cgccaacaat 900

gcagacgtcg agacgctgcg cgtttacggc gagcatcctt tttttaccct gttgcagcag 960

cgagccgcgc ttcagtggcc acagctgcgc gtggaacaac ggccggcatt accggggctg 1020

ggagcggccg tccagatcaa tgacgctttc accgtcagca tcaccgatgg ccgcacggcg 1080

agccaactgg ccgagcagac ggcagcggat gcctttgtgg tcgatgtcgc cctgaactac 1140

gccgacacca cgtatctggt ggcggcgcac agccgccacg cttctgcggc caataaggcg 1200

ctgtttttac gcctgctgca cacggcaatc ccgcaggttg aatttatcaa ggactccccg 1260

gcgcttatcg tcgcccgcgt cctcagcagc ctgatcaatg agtcggtgat catggtggaa 1320

agcggcgtct gcagccggga agacatcgat gtcgccgccg tcgcgggcgt taactacgcc 1380

ggcggcattt tcgactggct cggcaaactg ggggagaaaa acgtcaggac gacgctgagc 1440

aatctggcgc agctgctgca cgcggcgcgc tatgcgccgc attacaccct tctgcacgcc 1500

gcgcaaccgg cgctgacgac cacgccttaa 1530

<210> 110

<211> 1530

<212> DNA

<213> Serratia marcescens 1185

<400> 110

atggcagaaa gtaatgcggc aattcaatcg gctgcgatta tcggcgcggg aacgatgggc 60

agaggcatcg cttatctttt cgcgcaaaaa ggcattccca cgatgcttta taatcgcaac 120

ggcaataccc tcaatcaagc tcgcgaatat atcgcgcaag acctgaacaa gaaagtcgaa 180

cagggcaaga tcgcgctgcg ggataaagac gcggtgctgg ccaatctgat gttcacttcc 240

gtgtttgagg ccatcgccga cagcgagctg gtgatagaaa ccatcgccga gcaagaacaa 300

accaaacttg aggtgctggc ggccatcgcc gcggtggtca agcccgacac gctgatcgcc 360

accaatacct cctcactgtc gcttaacaag ctggctactg cggtgacgca cagcgaacgc 420

tttatcggtt tgcatttttt caaccccgcg ccgctgatga agctgattga aatcattccg 480

gcctacttta ccgcgcacgc caccaccgaa cgctgccgcc aactggtggc ggcgttgggg 540

aaacacgatg tcgtctgcca ggccacgccg gggttcatcg tcaatcgcat ggcccgcccc 600

tactacctgg aagggttccg cctgttggaa gaacatgtgg cgcgcgcggc gcagatcgac 660

cgcgccctca aggccggcgg gcgcttccgc atggggccgc tcgagctgac cgattttatc 720

ggccaagaca tcaactatca ggtcagccgg caaatctggc aggacatgca atacgacccg 780

cgctataccc ctggtcatct gcagcgttca ctggtcgatg ccggtctgtt ggggaaaaag 840

aacggccgct cttattttgc cgccgaagaa accgccccgc cggtgacggc cgccaacaat 900

gcagacgtcg agacgctgcg cgtttacggc gagcatcctt tttttaccct gttgcagcag 960

cgagccgcgc ttcagtggcc acagctgcgc gtggaacaac ggccggcatt accggggctg 1020

ggagcggccg tccagatcaa tgacgctttc accgtcagca tcaccgatgg ccgcacggcg 1080

agccaactgg ccgagcagac ggcagcggat gcctttgtgg tcgatctcgc cctgaactac 1140

gccgacacca cgtatctggt ggcggcgcac agccgccacg cttctgcggc caataaggcg 1200

ctgtttttac gcctgctgca cacggcaatc ccgcaggttg aatttatcaa ggactctccg 1260

gcgcttatcg tggcccgcgt cctcagcagc ctgatcaatg agtcggtgat catggtggaa 1320

agcggcgtct gcagccggga agacatcgat gtcgccgccg tcgcgggcgt taactacgcc 1380

ggcggcattt tcgactggct cggcaaactg ggggagaaaa acgtcaggac gacgctgagc 1440

aatctggcgc agctgctgca cgcggcgcgc tatgcgccgc attacaccct tctgcacgcc 1500

gcgcaatcgg cgctgacgac cacgccttaa 1530

<210> 111

<211> 1530

<212> DNA

<213> Serratia marcescens S2I7

<400> 111

atggcagaaa gtaatgcggc aattcaatcg gctgcgatta tcggcgcggg aacgatgggc 60

agaggcatcg cttatctttt cgcgcaaaaa ggcattccca cgatgcttta taatcgcaac 120

ggcaataccc tcaatcaagc tcgcgaatat atcgcgcaag atctgaataa gaaagtcgaa 180

cagggcaaga tcgcgctgca ggataaggac gcggtgctgg ccaatctgat gttcacttcc 240

gtgtttgagg ccatcgccga cagcgagctg gtgatagaaa ccatcgccga gcaagaacaa 300

accaaacttg aggtgctggc ggccatcgcc gcggtggtca agcccgacac gctgatcgcc 360

accaatacct cctcactgtc gcttaacaag ctggcaactg cggtgacgca cagcgaacgc 420

tttatcggtt tgcatttttt caaccccgcg ccgctgatga agctgattga aatcattccg 480

gcctacttta ccgcgcacgc caccacggaa cgctgccgcc aactggtggc ggcgttgggg 540

aaacgcgatg tcgtctgcca ggccacgccg gggttcatcg tcaatcgcat ggcccgcccc 600

tactacctgg aagggttccg cctgttggaa gaacatgtgg cgcgcgcggc gcagatcgac 660

cgcgccctca aggccggcgg gcgcttccgc atggggccgc tcgagctgac cgattttatc 720

ggccaagaca tcaactatca ggtcagccgg caaatctggc aggacatgca atacgacccg 780

cgctataccc ctggtcatct gcagcgttca ctggtcgatg ccggtctgtt ggggaaaaag 840

aacggccgct cctattttgc cgccgaagaa accgccccgc cggtgacggc cgccaacaat 900

gcagacgtcg agacgctgcg cgtttacggc gagcatcctt tttttaccct gttgcagcag 960

cgagccgcgc ttcagtggcc acagctgtgc gtggaacaac ggccggcatt accggggctg 1020

ggagcggccg tccagatcaa tgacgctttc accgtcagca tcaccgatgg ccgcacggcg 1080

agccaactgg ccgagcagac agcagcggat gcctttgtgg tcgatgtcgc cctgaactac 1140

gccgacacca cgtatctggt ggcagcgcac agccgccacg cttctgcggc caataaggcg 1200

ctgtttttac gcctgctgca cacggcaatc ccgcaggttg aatttatcaa ggactctccg 1260

gcgcttatcg tcgcccgcgt cctcagcagc ctgatcaatg agtcggtgat catggtggaa 1320

agcggcgtct gcagccggga agacatcgat gtcgccgccg tcgcgggcgt taactacgcc 1380

ggcggcattt tcgactggct cggcaaactg ggggagagaa acgtcaggac gacgctgagc 1440

aatctggcgc agctgctgca cgcggcgcgc tatgcgccgc attacaccct tctgcacgcc 1500

gcgcaaccgg cgctgacgac cacgccttaa 1530

<210> 112

<211> 1530

<212> DNA

<213> Serratia marcescens KHCo-24B

<400> 112

atggcagaaa gtaatgcggc aattcaatcg gctgcgatta tcggcgcggg aacgatgggc 60

agaggcatcg cttatctttt cgcgcaaaaa ggcattccca cgatgcttta taatcgcaac 120

ggcaataccc tcaatcaagc tcgcgaatat atcgcgcaag acctgaataa gaaagtcgaa 180

cagggcaaga tcgcgctgcg ggataaagac gcggtgctgg ccaatctgat gttcacttcc 240

gtgtttgagg ccatcgccga cagcgagctg gtgatagaaa ccatcgccga gcaagaacaa 300

accaaacttg aggtgctggc ggccatcgcc gcagtggtca agcccgacac gctgatcgcc 360

accaatacct cctcactgtc gctcaacaag ctggcaacgg cggtaacgca cagcgaacgc 420

tttatcggtt tgcatttttt caaccccgcg ccgctgatga agctgattga aatcattccg 480

gcctacttta ccgcgcacgc caccacggaa cgctgccgcc aactggtggc ggcgttgggg 540

aaacgcgatg tcgtctgcca ggccacgccg gggttcatcg tcaatcgcat ggcccgcccc 600

tactacctgg aagggttccg cctgttggaa gaacatgtgg cgcgcgcggc gcagatcgac 660

cgcgccctca aggctggcgg gcgcttccgc atggggccgc tcgagctgac cgattttatc 720

ggccaagaca tcaactatca ggtcagccgg caaatctggc aggacatgca atacgacccg 780

cgctataccc ctggtcatct gcagcgttca ctggtcgatg ccggtctgtt ggggaaaaag 840

aacggccgct cctattttgc cgccgaagaa accgccccgc cggtgacggc cgccaacaat 900

gcagacgtcg agacgctgcg cgtttacggc gagcatcctt tttttaccct gttgcagcag 960

cgagccgcgc ttcagtggcc acagctgcgc gtggaacaac ggccggcatt accggggctg 1020

ggagcggccg tccagatcaa tgacgctttc accgtcagca tcaccgatgg ccgcacggcg 1080

agccaactgg ccgagcaggc ggcagcggat gcctttgtgg tcgatctcgc cctgaactac 1140

gccgacacca cgtatctggt ggcggcgcac agccgccacg cttctgcggc caataaggcg 1200

ctgtttttac gcctgctgca cacggcaatc ccgcaggttg aatttatcaa ggactctccg 1260

gcgcttatcg tcgcccgcgt cctcagcagc ctgatcaatg agtcggtgat catggtggaa 1320

agcggcgtct gcagccggga agacatcgat gtcgccgccg tcgcgggcgt taactacgcc 1380

ggcggcattt tcgactggct cggcaaactg ggggagaaaa acgtcaggac gacgctgagc 1440

aatctggcgc agctgctgca cgcggcgcgc tatgcgccgc attacaccct tctgcacgcc 1500

gcgcaaccgg cgctgacgac cacgccttaa 1530

<210> 113

<211> 1530

<212> DNA

<213> Serratia marcescens Z6

<400> 113

atggcagaaa gtaatgcggc aattcaatcg gctgcgatta ttggcgcggg aacgatgggc 60

agaggcatcg cttatctttt cgcgcaaaaa ggcattccca cgatgcttta taatcgcaac 120

ggcaataccc tcaatcaagc tcgcgaatat atcgcgcaag acctgaacaa gaaagtcgaa 180

cagggcaaga tcgcgctgca ggataaagac gcggtgctgg ccaatctgat gttcacttcc 240

gtgtttgagg ccatcgccga cagcgagctg gtgatagaaa ccatcgccga gcaagaacaa 300

accaaacttg aggtgctggc ggccatcgcc gcggtggtca agcccgacac gctgatcgcc 360

accaatacct cctcactgtc gcttaacaag ctggcaactg cggtgacgca cagagagcgc 420

tttatcggtt tgcatttttt caaccccgcg ccgctgatga agctgattga aatcattccg 480

gcctacttta ccgcgcacgc caccacggaa cgctgccgcc aactggtggc ggcgttgggg 540

aaacgcgatg tcgtctgcca ggccacgccg gggttcatcg tcaatcgcat ggcccgcccc 600

tactacctgg aagggttccg cctgttggaa gaacatgtgg cgcgcgcggc gcagatcgac 660

cgcgccctca aggccggcgg gcgcttccgc atggggccgc tcgagctgac cgattttatc 720

ggccaagaca ttaactatca ggtcagccgg caaatctggc aggacatgca atacgacccg 780

cgctataccc ctggtcattt gcagcgttca ctggtcgatg ccggtctgtt ggggaaaaag 840

aacggccgct cctattttgc cgccgaagaa accgccccgc cggtgacggc cgccaacaat 900

gcagacgtcg agacgctgcg cgtttacggc gagcaccctt tttttaccct gttgcagcag 960

cgagccgcgc ttcagtggcc acagctgtgc gtggaacaac ggccggcatt accggggctg 1020

ggagcggccg tccagataaa tgacgctttc accgtcagca tcaccgatgg ccgcacggcg 1080

agccaactgg ccgagcagac ggcagcggat gcctttgtga tcgatgtcgc cctgaactac 1140

gccgacacca cgtatctggt ggcagcgcac agccgccacg cttctgcggc caataaggcg 1200

ctgtttttac gcctgctgca cacggcaatc ccgcaggttg aatttatcaa ggactccccg 1260

gcgcttatcg tcgcccgcgt cctcagcagc ctgatcaatg agtcggtgat catggtggaa 1320

agcggcgtct gcagccggga agacatcgat gtcgccgccg tcgcgggcgt taactacgcc 1380

ggcggcattt tcgactggct cggcaagctg ggggagaaaa acgtcaggac gacgctgagc 1440

aatctggcgc agctgctgca cgcggcgcgc tatgcgccgc attacaccct cctgcacgcc 1500

gcgcaaccgg cgctgacgac cacgccttaa 1530

<210> 114

<211> 1530

<212> DNA

<213> Serratia marcescens 546

<400> 114

atggcagaaa gtaatgcggc aattcaatcg gctgcgatta tcggcgcggg aacgatgggc 60

agaggcatcg cttatctttt cgcgcaaaaa ggcattccca cgatgcttta taatcgcaac 120

ggcaataccc taaatcaagc tcgcgaatat atcgcgcaag acctgaacaa gaaagtcgaa 180

cagggcaaga tcgcgctgcg ggataaagac gcggtgctgg ccaatctgat gttcacttcc 240

gtgtttgagg ccatcgccga cagcgagctg gtgatagaaa ccatcgccga gcaagaacaa 300

accaaacttg aggtgctggc ggccatcgcc gcagtggtca agcccgacac gctgatcgcc 360

accaatacct cctcactgtc gcttaacaag ctgtcaactg cggtaacgca caacgaacgc 420

tttatcggtt tgcatttttt caaccccgcg ccgctgatga agctgattga aatcattccg 480

gcctacttta ccgcgcacgc caccacggaa cgctgccgcc aactggtggc ggcgttgggg 540

aaacgcgatg tcgtctgcca ggccacgccg gggttcatcg tcaatcgcat ggcccgcccc 600

tactacctgg aagggttccg cctgttggaa gaacatgtgg cgcgcgcggc gcagatcgac 660

cgcgccctca aggccggcgg gcgcttccgc atggggccgc tcgagctgac cgattttatc 720

ggccaagaca tcaactatca ggtcagccgg caaatctggc aggacatgca atacgacccg 780

cgctataccc ctggtcatct gcagcgttca ctggtcgatg ccggtctgtt ggggaaaaag 840

aacggccgct cctattttgc cgccgaagaa accgccccgc cggtgacggc cgccaacaat 900

gcagacgtcg agacgctgcg cgtttacggc gagcaccctt tttttaccct gttgcagcag 960

cgagccgcgc ttcagtggcc acagctgcgc gtggaacaac ggccggcatt accggggctg 1020

ggagcggccg tccggatcaa tgacgctttc accgtcagca tcaccgatgg ccgcacggcg 1080

agccaactgg ccgagcagac ggcagcggat gcctttgtgg tcgatgtcgc cctgaactac 1140

gccgacacca cgtatctggt ggcggcgcac agccgccacg cttctgcggc caataaggcg 1200

ctgtttttac gcctgctgca cacggcaatc ccgcaggttg aatttatcaa ggactctccg 1260

gcgcttatcg tcgcccgcgt cctcagcagc ctgatcaatg agtcggtgat catggtggaa 1320

agcggcgtct gcagccggga agacatcgat gtcgccgccg tcgcgggcgt taactacgcc 1380

ggcggcattt tcgactggct cggcaaactg ggggagaaaa acgtcaggac gacgctgagc 1440

aatctggcgc agctgctgca cgcggcgcgc tatgcgccgc attacaccct tctgcacgcc 1500

gcgcaaccgg cgctgacgac cacgccttaa 1530

<210> 115

<211> 1530

<212> DNA

<213> Serratia nematodiphila MB307

<400> 115

atggcagaaa gtaatgcggc aattcaatcg gctgcgatta tcggcgcggg aacgatgggc 60

agaggcatcg cttatctttt cgcgcaaaaa ggcattccca cgatgcttta taatcgcaac 120

ggcaataccc tcaatcaagc tcgcgaatat atcgcgcaag acctgaataa gaaagtcgaa 180

cagggcaaga tcgcgctgcg ggataaagac gcggtgctgg ccaatctgat gttcacttcc 240

gtgtttgagg ccatcgccga cagcgagctg gtgatagaaa ccatcgccga gcaagaacaa 300

accaaacttg aggtgctggc ggccatcgcc gcagtggtca agcccgacac gctgatcgcc 360

accaatacct cctcactgtc gctcaacaag ctggcaacgg cggtaacgca cagcgaacgc 420

tttatcggtt tgcatttttt caaccccgcg ccgctgatga agctgattga aatcattccg 480

gcctacttta ccgcgcacgc caccacggaa cgctgccgcc aactggtggc ggcgttgggg 540

aaacgcgatg tcgtctgcca ggccacgccg ggtttcatcg tcaatcgcat ggcccgcccc 600

tactacctgg aagggttccg cctgttggaa gaacatgtgg cgcgcgcggc gcagatcgac 660

cgcgccctca aggctggcgg gcgcttccgc atggggccgc tcgagctgac cgattttatc 720

ggccaagaca tcaactatca ggtcagccgg caaatctggc aggacatgca atacgacccg 780

cgctataccc ctggtcatct gcagcgttca ctggtcgatg ccggtctgtt ggggaaaaag 840

aacggccgct cctattttgc cgccgaagaa accgccccgc aggtgacggc cgccaacaat 900

gcagacgtcg agacgctgcg cgtttacggc gagcatcctt tttttaccct gttgcagcag 960

cgagccgcgc ttcagtggcc acagctgcgc gtggaacaac ggccggcatt accggggctg 1020

ggagcggccg tccagatcaa tgacgctttc accgtcagca tcaccgatgg ccgcacggcg 1080

agccaactgg ccgagcagac ggcagcggat gcctttgtgg tcgatctcgc cctgaactac 1140

gccgacacca cgtatctggt ggcggcgcac agccgccacg cttctgcggc caataaggcg 1200

ctgtttttac gcctgctgca cacggcaatc ccgcaggttg aatttatcaa ggactctccg 1260

gcgcttatcg tcgcccgcgt cctcagcagc ctgatcaatg agtcggtgat catggtggaa 1320

agcggcgtct gcagccggga agacatcgat gtcgccgccg tcgcgggcgt taactacgcc 1380

ggcggcattt tcgactggct cggcaaactg ggggagaaaa acgtcaggac gacgctgagc 1440

aatctggcgc agctgctgca cgcggcgcgc tatgcgccgc attacaccct tctgcacgcc 1500

gcgcaaccgg cgctgacgac cacgccttaa 1530

<210> 116

<211> 1530

<212> DNA

<213> Serratia marcescens VGH107

<400> 116

atggcagaaa gtaatgcggc aattcaatcg gctgcgatta tcggcgcggg aacgatgggc 60

agaggcatcg cttatctttt cgcgcaaaaa ggcattccca cgatgcttta taatcgcaac 120

ggcaataccc tcaatcaagc tcgcgaatat atcgcgcaag acctgaataa gaaagtcgaa 180

cagggcaaga tcgcgctgca ggataaggac gcggtgctgg ccaatctgat gttcacttcc 240

gtgtttgagg ccatcgccga cagcgagctg gtaatagaaa ccatcgccga gcaagaacaa 300

accaaacttg aggtgctggc ggccatcgcc gcggtggtca agcacgacac gctgatcgcc 360

accaatacct cctcactgtc gctcaacaag ctggcaactg cggtgacgca cagcgaacgc 420

tttatcggtt tgcatttttt caaccccgcg ccgctgatga agctgattga aatcattccg 480

gcctacttta ccgcgcacgc caccacggaa cgctgccgcc aactggtggc ggcgttgggg 540

aaacgcgatg tcgtctgcca ggccacgccg gggttcatcg tcaatcgcat ggcccgcccc 600

tactacctgg aagggttccg cctgttggaa gaacatgtgg cgcgcgcggc gcagatcgac 660

cgcgccctca aggccggcgg gcgcttccgc atggggccgc tcgagctgac cgattttatt 720

ggccaagaca ttaactatca ggtcagccgg caaatctggc aggacatgca atacgacccg 780

cgctataccc ctggtcatct gcagcgttca ctggtcgatg ccggtctgtt ggggaaaaag 840

aacggccgct cctattttgc cgccgaagaa accgccccgc cggtgacggc cgccaacaat 900

gcagacgtcg agacgctgcg cgtttacggc gagcatcctt tttttaccct gttgcagcag 960

cgagccgcgc ttcagtggcc acagctgcgc gtggaacaac ggccggcatt accggggctg 1020

ggagcggccg tccagatcaa tgacgctttc accgtaagca tcaccgatgg ccgcacggcg 1080

agccaactgg ccgagcagac ggcagcggat gcctttgtgg tcgatgtcgc cctgaactac 1140

gccgacacca cgtatctggt ggcggcgcac agccgccacg cttctgcggc caataaggcg 1200

ctgtttttgc gcctgctgca cacggcaatc ccgcaggttg aatttatcaa ggactctccg 1260

gcgcttatcg tcgcccgcgt cctcagcagc ctgatcaatg agtcggtgat catggtggaa 1320

agcggcgtct gcagccggga agacatcgat gtcgccgccg tcgcgggcgt taactacgcc 1380

ggcggcattt tcgactggct cggcaaactg ggggagaaaa acgtcaggac gatgctgagc 1440

aatctggcgc agctgctgca cgcggcgcgc tatgcgccgc attacaacct tctgcacgcc 1500

gcgcaaccgg cgctgacgac cacgccttaa 1530

<210> 117

<211> 1530

<212> DNA

<213> Serratia marcescens MCB

<400> 117

atggcagaaa gtaatgcggc aattcaatcg gctgcgatta tcggcgcggg aacgatgggc 60

agaggcatcg cttatctttt cgcgcaaaag ggcattcgta cggtgcttta taatcgcaac 120

ggcaataccc tcaatcaggc tcgcgaatat atcgcgcaag acctgaacaa gaaagtcgaa 180

cagggcaaga tcgcgctgca ggataaaagc tcagtgctgg ccaatctggt gttctcgacg 240

gcgcttgagg ctatcgccga cagcgagttg gtgatagaaa ccatcgccga gcatgaacaa 300

accaaacttg aggtgttggc ggccatcgcc gcggtggtca agcccgatac gctgatcgcc 360

accaatacct cctcactgtc gcttaacaag ctggcaaccg cggtgacgca cagcgagcgg 420

tttatcggtt tgcacttttt caaccctgcg ccgctgatga aactgattga aatcatcccg 480

gcctacttta ccgcacacgc caccacggaa cgttgccgtc aactggtggc cgcgttgggg 540

aaacgcgatg tcgtctgtca ggccacgccg gggtttatcg ttaaccgcat ggcccgcccc 600

tactacctgg aagggttccg cctgttggaa gaacacgtgg cgcgcgcgcc gcagatcgac 660

cgtgccctca aggccggcgg acacttccgc atggggccgc tcgagctgac cgatttcatc 720

ggccaggaca tcaactatca ggtcagtcgg caaatctggc aggacatgca atacgacccg 780

cgctataccc ccggccatct gcagcgttcg ctggtcgatg ccggtctgtt ggggaaaaag 840

aacggccgtt cctattttac cgccgaagaa accgccccgc cggtgacggc tgccatcggt 900

gcagacgtcg agacgctgcg catttacggc gaacatcctt tttttaccct gttgcaacag 960

cgggccgcac ttcagtggcc acagctgcgc gtggaacaac ggccggcctt accaggcgtg 1020

gggccggccg tccagatcaa tgacgcattc accgtcagca tcaccgacgg ccgtacggcg 1080

agccagttag ccgagcagac gacggctgac gcttttgtgg tcgatatcgc cctgaactac 1140

gccgacacgg cgtacctggt tgcggcgcac aatcgccacg cctctgcggc caataaggcg 1200

ctgtttttac gcctgttgca caccgcaatt ccgcaggttg aatttatcaa agactccccg 1260

gcgctgatcg tcgctcgcgt cctcagcagc ctgatcaatg agtcggtgat catggtggaa 1320

agcggcgttt gcagccggga agacattgat gtcgccgccg tcgcgggcgt taactacgcc 1380

ggcggcattt tcgactggct cagcaagctg ggggagaaaa acgtcaggac gacgctgagc 1440

aatctggcgc agttgctgca cgcggcgcgt tatgcgccgc attacaccct tctgcacgcc 1500

gcgcaaccgg cgctgacgac cacgccttga 1530

<210> 118

<211> 1530

<212> DNA

<213> Serratia marcescens AH0650

<400> 118

atggcagaaa gtaatgcggc aattcaatcg gctgcgatta tcggcgcggg aacgatgggc 60

agaggcatcg cttatctttt cgcgcaaaaa ggcattcgta cggtgcttta taatcgcaac 120

ggcaataccc tcaatcaggc tcgcgaatat atcgcgcaag acctgaacaa gaaagtcgaa 180

cagggcaaga tcgcgctgca ggataaaagc tcagtgctgg ccaatctggt gttctcgacg 240

gcgcttgagg ctatcgccga cagcgagttg gtgatagaaa ccatcgccga gcatgaacaa 300

accaaacttg aggtgttggc ggccatcgcc gcggtggtca agcccgatac gctgatcgcc 360

accaatacct cctcactgtc gcttaacaag ctggcaaccg cggtgacgca cagcgagcgg 420

tttatcggtt tgcacttttt caaccctgcg ccgctgatga aactgattga aatcatcccg 480

gcctacttta ccgcacacgc caccacggaa cgttgccgtc aactggtggc cgcgttgggg 540

aaacgcgatg tcgtctgtca ggccacgccg gggtttatcg ttaaccgcat ggcccgcccc 600

tactacctgg aagggttccg cctgttggaa gaacacgtgg cgcgcgcgcc gcagatcgac 660

cgtgccctca aggccggcgg acacttccgc atggggccgc tcgagctgac cgattttatc 720

ggccaggaca tcaactatca ggtcagccgg caaatctggc aggacatgca atacgacccg 780

cgctataccc ccggccatct gcagcgttcg ctggtcgatg ccggtctgtt ggggaaaaag 840

aacggccgct cctattttgc cgccgaagaa accgccccgc cagtgacggc cgccatcgat 900

gcagacgtcg agacgctgcg cgtttacggc gaacaccctt tttttaccct gttgcaacag 960

cgagccgcac gtcagtggcc acagctgcgc gtggaacaac ggccggcctt accgagcctg 1020

gggccggccg tccagatcaa tgacgcattc accgtcagca tcaccgatgg ccgcacggcg 1080

agccagttag ccgagcagac gacggctgac gcttttgtgg tcgacatcgc cctgaactac 1140

gccgacacgg cgtatctggt tgcggcgcac aatcgctatg cctctgcggc caataaggcg 1200

ctgtttttac gcctgctgca cactgcgatc ccgcaggttg aatttatcaa agactccccg 1260

gcgctgatcg tcgctcgtgt cctcagcagc ctgatcaatg agtcggtgat catggtggaa 1320

agcggcgttt gcagccggga agacattgat gtcgccgccg tcgcgggcgt taactacgcc 1380

ggcggcattt tcgactggct cggcaaactg ggggagaaaa acgtcaggac gacgctgagc 1440

aatctggcgc agctgctgca cgcagcgcgc tatgcgccgc attacaccct tctgcacgcc 1500

gcgcaaccgg cgctgacgac cacgccttga 1530

<210> 119

<211> 1530

<212> DNA

<213> Serratia marcescens UMH12

<400> 119

atggcagaaa gtaatgcgga aattcaatcg gctgcgatta tcggcgcggg aacgatgggc 60

agaggcatcg cttatctttt cgcgcaaaaa ggcattcgta cggtgcttta taatcgcaac 120

ggcaataccc tcaatcaggc tcgcgaatat atcgcgcaag acctgaacaa gaaagtcgaa 180

cagggcaaga tcgcgctgca ggataaaagc tcagtgctgg ccaatctggt gttctcgacg 240

gcgcttgagg ctatcgccga cagcgagttg gtgatagaaa ccatcgccga gcatgaacaa 300

accaaacttg aggtgttggc ggccatcgcc gcggtggtca agcccgatac gctgatcgcc 360

accaatacct cctcactgtc gcttaacaag ctggcaaccg cggtgacgca cagcgagcgg 420

tttatcggtt tgcacttttt caaccctgcg ccgctgatga aactgattga aatcatcccg 480

gcctacttta ccgcacacgc caccacggaa cgttgccgtc aactggtggc cgcgttgggg 540

aaacgcgatg tcgtctgtca ggccacgccg gggtttatcg ttaaccgcat ggcccgcccc 600

tactacctgg aagggttccg cctgttggaa gaacacgtgg cgcgcgcgcc gcagatcgac 660

cgtgccctca aggccggcgg acacttccgc atggggccgc tcgagctgac cgattttatc 720

ggccaggaca tcaactatca ggtcagccgg caaatctggc aggacatgca atacgacccg 780

cgctataccc ccggccatct gcagcgttcg ctggtcgatg ccggtctgtt ggggaaaaag 840

aacggccgct cctattttgc cgccgaagaa accgccccgc cagtgacggc cgccatcgat 900

gcagacgtcg agacgctgcg cgtttacggc gaacaccctt tttttaccct gttgcaacag 960

cgagccgcac gtcagtggcc acagctgcgc gtggaacaac ggccggcctt accgagcctg 1020

gggccggccg tccagatcaa tgacgcattc accgtcagca tcaccgatgg ccgcacggcg 1080

agccagttag ccgagcagac gacggctgac gcttttgtgg tcgacatcgc cctgaactac 1140

gccgacacgg cgtatctggt tgcggcgcac aatcgctatg cctctgcggc caataaggcg 1200

ctgtttttac gcctgctgca cactgcgatc ccgcaggttg aatttatcaa agactccccg 1260

gcgctgatcg tcgctcgtgt cctcagcagc ctgatcaatg agtcggtgat catggtggaa 1320

agcggcgttt gcagccggga agacattgat gtcgccgccg tcgcgggcgt taactacgcc 1380

ggcggcattt tcgactggct cggcaaactg ggggagaaaa acgtcaggac gacgctgagc 1440

aatctggcgc agctgctgca cgcagcgcgc tatgcgccgc attacaccct tctgcacgcc 1500

gcgcaaccgg cgctgacgac cacgccttga 1530

<210> 120

<211> 1530

<212> DNA

<213> Serratia sp. OMLW3

<400> 120

atggcagaaa gtaatgcggc aattcaatcg gctgcgatta tcggcgcggg aacgatgggc 60

agaggcatcg cttatctttt cgcgcaaaaa ggcattcgta cggtgcttta taatcgcaac 120

ggcaataccc tcaatcaggc tcgcgaatat atcgcgcaag acctgaacaa gaaagtcgaa 180

cagggcaaga tcgcgctgca ggataaaagc tcagtgctgg ccaatctggt gttctcgacg 240

gcgcttgtgg ctatcgccga cagcgagttg gtgatagaaa ccatcgccga gcatgaacaa 300

accaaacttg aggtgttggc ggccatcgcc gcggtggtca agcccgatac gctgatcgcc 360

accaatacct cctcactgtc gcttaacaag ctggcaaccg cggtgacgca cagcgagcgg 420

tttatcggtt tgcacttttt caaccctgcg ccgctgatga aactgattga aatcatcccg 480

gcctacttta ccgcacacgc caccacggaa cgttgccgtc aactggtggc cgcgttgggg 540

aaacgcgatg tcgtctgtca ggccacgccg gggtttatcg tcaaccgcat ggcccgcccc 600

tactacctgg aagggttccg cctgttggaa gagcacgtgg cgcgcgcgcc gcagatcgac 660

cgtgccctca aggccggcgg acacttccgc atggggccgc tcgagctgac cgattttatc 720

ggccaggaca tcaactatca ggtcagccgg caaatctggc aggacatgca atacgacccg 780

cgctataccc ccggccatct gcagcgttcg ctggtcgatg ccggtctgtt ggggaaaaag 840

aacggccgct cctattttgc cgccgaagaa accgccccgc cagtgacggc cgccatcgat 900

gcagacgtcg agacgctgcg cgtttacggc gaacaccctt tttttaccct gttgcaacag 960

cgagccgcac gtcagtggcc acagctgcgc gtggaacaac ggccggcctt accgagcctg 1020

gggccggccg tccagatcaa tgacgcattc accgtcagca tcaccgatgg ccgcacggcg 1080

agccagttag ccgagcagac gacggctgac gcttttgtgg tcgacatcgc cctgaactac 1140

gccgacacgg cgtatctggt tgcggcgcac aatcgctatg cctctgcggc caataaggcg 1200

ctgtttttac gcctgctgca cactgcgatc ccgcaggttg aatttatcaa agattccccg 1260

gcgctgatcg tcgctcgcgt cctcagcagc ctgatcaatg agtcggtgat catggtggaa 1320

agcggcgtct gcagccggga agacattgat gtcgccgccg tcgcgggcgt taactacgcc 1380

ggcggcattt tcgactggct cggcaaactg ggggagaaaa acgtcaggac gacgctgagc 1440

aatctggcgc agctgctgca cgcagcgcgc tatgcgccgc attacaccct tctgcacgcc 1500

gcgcaaccgg cgctgacgac cacgccttga 1530

<210> 121

<211> 1530

<212> DNA

<213> Serratia marcescens UMH11

<400> 121

atggcagaaa gtaatgcggc aattcaatcg gctgcgatta tcggcgcggg aacgatgggc 60

agaggcatcg cttatctttt cgcgcaaaaa ggcattcgta cggtgcttta taatcgcagc 120

ggcaataccc tcaatcaggc tcgcgaatat atcgcgcaag acctgaacaa gaaagtcgaa 180

cagggcaaga tcgcgctgca ggataaaagc tcagtgctgg ccaatctggt gttctcgacg 240

gcgcttgtgg ctatcgccga cagcgagttg gtgatagaaa ccatcgccga gcatgaacaa 300

accaaacttg aggtgttggc ggccatcgcc gcggtggtca agcccgatac gctgatcgcc 360

accaatacct cctcactgtc gcttaacaag ctggcaaccg cggtgacgca cagcgagcgg 420

tttatcggtt tgcacttttt caaccctgcg ccgctgatga aactgattga aatcatcccg 480

gcctacttta ccgcacacgc caccacggaa cgttgccgtc aactggtggc cgcgttgggg 540

aaacgcgatg tcgtctgtca ggccacgccg gggtttatcg tcaaccgcat ggcccgcccc 600

tactacctgg aagggttccg cctgttggaa gagcacgtgg cgcgcgcgcc gcagatcgac 660

cgtgccctca aggccggcgg acacttccgc atggggccgc tcgagctgac cgattttatc 720

ggccaggaca tcaactatca ggtcagccgg caaatctggc aggacatgca atacgacccg 780

cgctataccc ccggccatct gcagcgttcg ctggtcgatg ccggtctgtt ggggaaaaag 840

aacggccgct cctattttgc cgccgaagaa accgccccgc cagtgacggc cgccatcgat 900

gcagacgtcg agacgctgcg cgtttacggc gaacaccctt tttttaccct gttgcaacag 960

cgagccgcac gtcagtggcc acagctgcgc gtggaacaac ggccggcctt accgagcctg 1020

gggccggccg tccagatcaa tgacgcattc accgtcagca tcaccgatgg ccgcacggcg 1080

agccagttag ccgagcagac gacggctgac gcttttgtgg tcgacatcgc cctgaactac 1140

gccgacacgg cgtatctggt tgcggcgcac aatcgctatg cctctgcggc caataaggcg 1200

ctgtttttac gcctgctgca cactgcgatc ccgcaggttg aatttatcaa agattccccg 1260

gcgctgatcg tcgctcgcgt cctcagcagc ctgatcaatg agtcggtgat catggtggaa 1320

agcggcgtct gcagccggga agacattgat gtcgccgccg tcgcgggcgt taactacgcc 1380

ggcggcattt tcgactggct cggcaaactg ggggagaaaa acgtcaggac gacgctgagc 1440

aatctggcgc aactgctgca cgcagcgcgc tatgcgccgc attacaccct tctgcacgcc 1500

gcgcaaccgg cgctgacgac cacgccttga 1530

<210> 122

<211> 1530

<212> DNA

<213> Serratia marcescens UMH1

<400> 122

atggcagaaa gtaatgcggc aattcaatcg gctgcgatta tcggcgcggg aacgatgggc 60

agaggtatcg cttatctttt cgcgcaaaaa ggcattcgta cggtgcttta taatcgcaac 120

ggcaataccc tcaatcaggc tcgcgaatat atcgcgcaag acctgaacaa gaaagtcgaa 180

cagggcaaga tcgcgctgca ggataaaagc tcagtgctgg ccaatctggt gttctcgacg 240

gcgcttgagg ctatcgccga cagcgagttg gtgatagaaa ccatcgctga gcatgaacaa 300

accaaacttg aggtgttggc ggccatcgcc gcggtggtca agcccgatac gctgatcgcc 360

accaatacct cctcactgtc gcttaacaag ctggcaaccg cggtgacgca cagcgagcgg 420

tttatcggtt tgcacttttt caaccctgcg ccgctgatga aactgattga aatcatcccg 480

gcctacttta ccgcacatgc caccacggaa cgttgccgtc aactggtggc cgcgttgggg 540

aaacgcgatg tcgtctgcca ggccacgccg gggtttatcg ttaaccgcat ggcccgcccc 600

tactacctgg aagggttccg cctgttggaa gaacacgtgg cgcgcgcgcc gcagatcgac 660

cgtgccctca aggccggcgg acacttccgc atggggccgc tcgagctgac cgattttatc 720

ggccaggaca tcaactatca ggtcagccgg caaatctggc aggacatgca atacgacccg 780

cgctataccc ccggccatct gcagcgttcg ctggtcgatg ccggtctgtt ggggaaaaag 840

aacggccgct cctattttgc cgccgaagaa accgccccgc cagtgacggc cgccatcgat 900

gcagacgtcg agacgctgcg cgtttacggc gaacaccctt tttttaccct gttgcaacag 960

caagccgcac gtcagtggcc acagctgcgc gtggaacaac ggccggcctt accgagcctg 1020

gggccggccg tccagatcaa tggcgcattc accgtcagca tcaccgatgg ccgcacggcg 1080

agccagttag ccgagcagac gacggctgac gcttttgtga tcgacatcgc cctgaactac 1140

gccgacacgg cgtatctggt tgcggcgcac aatcgctatg cctctgcggc caataaggcg 1200

ctgtttttac gcctgctgca cactgcgatc ccgcaggttg aatttatcaa agactccccg 1260

gcgctgatcg tcgctcgtgt cctcagcagc ctgatcaatg agtcggtgat catggtggaa 1320

agcggcgttt gcagccggga agacattgat gtcgccgccg tcgcgggcgt taactacgcc 1380

ggcggcattt tcgactggct cggcaaactg ggggagaaaa acgtcaggac gacgctgagc 1440

aatctggcgc agctgctgca cgcagcgcgc tatgcgccgc attacaccct tctgcacgcc 1500

gcgcaaccgg cgctgacgac cacgccttga 1530

<210> 123

<211> 1530

<212> DNA

<213> Serratia marcescens 2880STDY5683020

<400> 123

atggcagaaa gtaatgcggc aattcaatcg gctgcgatta tcggcgcggg aacgatgggc 60

agaggcatcg cttatctttt cgcgcaaaaa ggcattcgca cggtgcttta taatcgcaac 120

ggcaataccc tcaatcaggc tcgcgaatat atcgcgcaag acctgaacaa gaaagtcgaa 180

cagggcaaga tcgcgctgca ggataaaggc gcagtgctgg ccaatctggt gttcacctcg 240

gtgtttgaga ctatcgccga cagcgatctg gtgatagaaa ccatcgccga gcaagagcaa 300

accaaacttg aggtgctggc ggccatcgcc gcggcggtca agcccgacac gctgatcgcc 360

accaatacct cctcactgtc gcttaacaag ctggcaaccg cggtgacgca cagcgagcgg 420

tttatcggtt tgcacttttt caatcccgcg ccgctgatga aactgattga aatcatcccg 480

gcctacttta ccgcacgcgc caccaccgaa cgttgccgtc agctggtagc cgcgttgggg 540

aaacgcgatg tcgtctgcca ggccacgccg gggtttatcg tcaaccgcat ggcccgcccc 600

tactacctgg aagggttccg cctgttggaa gaacacgtgg cgcgcgcgcc gcagatcgac 660

cgcgccctca aggccggcgg gcactttcgc atggggccgc tcgagctgac cgattttatc 720

ggccaggaca tcaactatca ggtcagccgg caaatctggc aggacatgca atacgatccg 780

cgctataccc ccggccatct gcagcgttcg ctggtcgatg ccggtctgtt ggggaaaaag 840

aacggccgct cctattttgc cgccgaagaa aacgccttac cggtaacggc cgccaccgat 900

gcagacgtcg agacgctgcg cgtttacggc gaacaccctt tttttaccct gttgcaacag 960

cgggccgcgc ttcagtggcc acggctgcgc gtggagcaac ggccggcctt accgggcctg 1020

gggccggccg tccagatcaa tgaggctttc accgtcagcg tcaccgatgg ccgcacggcg 1080

agccagttgg cggagcagac ggcggcggat gcctttgtgg tcgatgtcgc cctgaattac 1140

gccgacacgg cgtatctggt ggcagcgcac aaccgccacg cctctgcggc caataaggcg 1200

ctgtttttac gcctgctgca cacggcaatc ccgcaggttg aatttatcaa ggactccccg 1260

gcgctgatcg tcgcccgcgt cctcagcagc ctgatcaacg agtcggtgat catggtggaa 1320

agcggcgtct gcagccggga agacatcgat gtcgccgccg tcgccggcgt taactacgcc 1380

gacggcattt tcggctggct cgatagcctg ggggagaaaa acgtcaggac gacgctgagc 1440

aacctggcgc agctgctgca cgcggcacgc tatgcgccgc attacaccct tctgcacgcc 1500

gcgcaaccgg cgctgacgac cacgccttaa 1530

<210> 124

<211> 1530

<212> DNA

<213> Serratia marcescens 99

<400> 124

atggcagaaa gtaatgcggc aattcaatcg gctgcgatta tcggtgcggg aacgatgggc 60

agaggcatcg cttatctttt cgcgcaaaaa ggcattcgca cggtgcttta taatcgcaac 120

ggcaataccc tcaatcaggc tcgcgaatat atcgcgcaag acctgaacaa gaaagtcgaa 180

cagggcaaga tcgcgctgca ggataaaggc gcagtgctgg ccaatctggt gttcacctcg 240

gtgtttgaga ctatcgccga cagcgatctg gtgatagaaa ccatcgccga gcaagagcaa 300

accaaacttg aggtgctggc ggccatcgcc gcggcggtca agcccgacac gctgatcgcc 360

accaatacct cctcactgtc gcttaacaag ctggcaaccg cggtgacgca cagcgagcgg 420

tttatcggtt tgcacttttt caatcccgcg ccgctgatga aactgattga aatcatcccg 480

gcctacttta ccgcacgcgc caccaccgaa cgttgccgtc agctggtggc cgcgttgggg 540

aaacgcgatg tcgtctgcca ggccacgccg gggtttatcg tcaaccgcat ggcccgcccc 600

tactacctgg aagggttccg cctgttggaa gaacacgtgg cgcgcgcgcc gcagatcgac 660

cgcgccctca aggccggcgg gcactttcgc atggggccgc tcgagctgac cgattttatc 720

ggccaggaca tcaactatca ggtcagccgg caaatctggc aggacatgca atacgatccg 780

cgctataccc ccggccatct gcagcgttcg ctggtcgatg ccggtctgtt ggggaaaaag 840

aacggccgct cctattttgc cgccgaagaa aacgccttac cggtaacggc cgcctccaat 900

gcagacgtcg agacgctgcg cgtttacggc gaacaccctt tttttaccct gttgcaacag 960

cgggccgcgc ttcagtggcc acggctgcgc gtggagcaac ggccggcctt accgggtctg 1020

gggccggccg tccagatcaa tgaggctttc accgtcagcg tcaccgatgg ccgcacggcg 1080

agccaattgg cggagctgac ggcggcggat gcctttgtgg tcgatgtcgc cctgaactac 1140

gccgacacgg cgtatctggt ggcagcgcac aaccgccacg cctctgcggc caataaggcg 1200

ctgtttttac gcctgctgca cacggcaatc ccgcaggttg aatttatcaa ggactccccg 1260

gcgctgatcg tcgcccgcgt cctcagcagc ctgatcaacg agtcggtgat catggtggaa 1320

agcggcgtct gcagccggga agacatcgat gtcgccgccg tcgccggcgt taactacgcc 1380

gacggcattt tcggctggct cgatagcctg ggggagaaaa acgtcaggac gacgctgagc 1440

aacctggcgc agctgctgca cgcggcacgc tatgcgccgc attacaccct tctacacgcc 1500

gcgcaaccgg tgctgacgac cacgccttaa 1530

<210> 125

<211> 1530

<212> DNA

<213> Serratia marcescens 374

<400> 125

atggcagaaa gtaatgcggc aattcaatcg gctgcgatta tcggcgcggg aacgatgggc 60

agaggcatcg cttatctttt cgcgcaaaag ggcattcgta cggtgcttta taatcgcaac 120

ggcaataccc tcaatcaggc tcgcgaatat atcgcgcaag acctgaacaa gaaagtcgaa 180

cagggcaaga tcgcgctgca ggataaaagc tcagtgctgg ccaatctggt gttctcgacg 240

gcgcttgtgg ctatcgccga cagcgagttg gtgatagaaa ccatcgccga gcatgaacaa 300

accaaacttg aggtgttggc ggccatcgcc gcggtggtca agcccgatac gctgatcgcc 360

accaatacct cctcactgtc gcttaacaag ctggcaaccg cggtgacgca cagcgagcgg 420

tttatcggtt tgcacttttt caaccctgcg ccgctgatga aactgattga aatcatcccg 480

gcctacttta ccgcacacgc caccacggaa cgttgccgtc aactggtggc cgcgttgggg 540

aaacgcgatg tcgtctgtca ggccacgccg gggtttatcg tcaaccgcat ggcccgcccc 600

tactacctgg aagggttccg cctgttggaa gagcacgtgg cgcgcgcgcc gcagatcgac 660

cgtgccctca aggccggcgg acacttccgc atggggccgc tcgagctgac cgattttatc 720

ggccaggaca tcaactatca ggtcagccgg caaatctggc aggacatgca atacgacccg 780

cgctataccc ccggccatct gcagcgttcg ctggtcgatg ccggtctgtt ggggaaaaag 840

aacggccgct cctattttgc cgccgaagaa accgccccgc cagtgacggc cgccatcgat 900

gcagacgtcg agacgctgcg cgtttacggc gaacaccctt tttttaccct gttgcaacag 960

caagccgcac gtcagtggcc acagctgcgc gtggaacaac ggccggcctt accgagcctg 1020

gggccggccg tccagatcaa tggcgcattc accgtcagca tcaccgatgg ccgcacggcg 1080

agccagttag ccgagcagac gacggctgac gcttttgtga tcgacatcgc cctgaactac 1140

gccgacacgg cgtatctggt tgcggcgcac aatcgctatg cctctgcggc caataaggcg 1200

ctgtttttac gcctactgca cactgcgatc ccgcaggttg aatttatcaa agactccccg 1260

gcgctgatcg tcgctcgtgt cctcagcagc ctgatcaatg agtcggtgat catggtggaa 1320

agcggcgttt gcagccggga agacattgat gtcgccgccg tcgcgggcgt taactacgcc 1380

ggcggcattt tcgactggct cggcaaactg ggggagaaaa acgtcaggac gacgctgagc 1440

aatctggcgc agctgctgca cgcagcgcgc tatgcgccgc attacaccct tctgcacgcc 1500

gcgcaaccgg cgctgacgac cacgccttga 1530

<210> 126

<211> 1530

<212> DNA

<213> Serratia marcescens 2880STDY5683036

<400> 126

atggcagaaa gtaatgcggc aattcaatcg gctgcgatta tcggtgcggg aacgatgggc 60

agaggcatcg cttatctttt cgcgcaaaaa ggcattcgca cggtgcttta taatcgcaac 120

ggcaataccc tcaatcaggc tcgcgaatat atcgcgcaag acctgaacaa gaaagtcgaa 180

cagggcaaga tcgcgctgca ggataaaggc gcagtgctgg ccaatctggt gttcacctcg 240

gtgtttgaga ctatcgccga cagcgatctg gtgatagaaa ccatcgccga gcaagagcaa 300

accaaacttg aggtgctggc ggccatcgcc gcggcggtca agcccgacac gctgctcgcc 360

accaatacct cctcactgtc gcttaacaag ctggcaaccg cggtgacgca cagcgagcgg 420

tttatcggtt tgcacttttt caatcccgcg ccgctgatga aactgattga aatcatcccg 480

gcctacttta ccgcacgcgc caccaccgaa cgttgccgtc agctggtggc cgcgttgggg 540

aaacgcgatg tcgtctgcca ggccacgccg gggtttatcg tcaaccgcat ggcccgcccc 600

tactacctgg aagggttccg cctgttggaa gaacacgtgg cgcgcgcgcc gcagatcgac 660

cgcgccctca aggccggcgg gcactttcgc atggggccgc tcgagctgac cgattttatc 720

ggccaggaca tcaactatca ggtcagccgg caaatctggc aggacatgca atacgatccg 780

cgctataccc ccggccatct gcagcgttcg ctggtcgatg ccggtctgtt ggggaaaaag 840

aacggccgct cctattttgc cgccgaagaa aacgccttac cggtaacggc cgcctccaat 900

gcagacgtcg agacgctgcg cgtttacggc gaacaccctt tttttaccct gttgcaacag 960

cgggccgcgc ttcagtggcc acggctgcgc gtggagcaac ggccggcctt accgggtctg 1020

gggccggccg tccagatcaa tgaggctttc accgtcagcg tcaccgatgg ccgcacggcg 1080

agccaattgg cggagctgac ggcggcggat gcctttgtgg tcgatgtcgc cctgaactac 1140

gccgacacgg cgtatctggt ggcagcgcac aaccgccacg cctctgcggc caataaggcg 1200

ctgtttttac gcctgctgca cacggcaatc ccgcaggttg aatttatcaa ggactccccg 1260

gcgctgatcg tcgcccgcgt cctcagcagc ctgatcaacg agtcggtgat catggtggaa 1320

agcggcgtct gcagccggga agacatcgat gtcgccgccg tcgccggcgt taactacgcc 1380

gacggcattt tcggctggct cgatagcctg ggggagaaaa acgtcaggac gacgctgagc 1440

aacctggcgc agctgctgca cgcggcacgc tatgcgccgc attacaccct tctacacgcc 1500

gcgcaaccgg tgctgacgac cacgccttaa 1530

<210> 127

<211> 1530

<212> DNA

<213> Serratia marcescens 2880STDY5683034

<400> 127

atggcagaaa gtaatgcggc aattcaatcg gctgcgatta tcggcgcggg aacgatgggc 60

agaggcatcg cttatctttt cgcgcaaaaa ggcattcgca cggtgcttta taatcgcaac 120

ggcaataccc tcaatcaggc tcgcgaatat atcgcgcaag acctgaacaa gaaagtcgaa 180

cagggcaaga tcgcgctgca ggataaaggc gcagtgctgg ccaatctggt gttcacctcg 240

gtgtttgaga ctatcgccga cagcgagctg gtgatagaaa ccatcgccga gcaagagcaa 300

accaaacttg aggtgctggc ggccatcgcc gcggcggtca agcccgacac gctgatcgcc 360

accaatacct cctcactgtc gcttaacaag ctggcaaccg cggtgacgca cagcgagcgg 420

tttatcggtt tgcacttttt caatcccgcg ccgctgatga aactgattga aatcatcccg 480

gcctacttta ccgcacgcgc caccaccgaa cgctgccgtc agttggtggc cgcgttgggg 540

aaacgcgatg tcgtctgcca ggccacgccg gggtttatcg tcaaccgcat ggcccgcccc 600

tactacctgg aagggttccg cctgttggaa gaacacgtgg cgcgcgcgcc gcagatcgac 660

cgcgccctca aggccggcgg gcactttcgc atggggccgc tcgagctgac cgattttatc 720

ggccaggaca tcaactatca ggtcagccgg caaatctggc aggacatgca atacgatccg 780

cgctataccc ccggccatct gcagcgttcg ctggtcgatg ccggtctgtt ggggaaaaag 840

aacggccgct cctattttgc cgccgaagaa aacgccttac cggtaacggc cgccaccgat 900

gcagacgtcg agacgctgcg catttacggc gaacaccctt tttttaccct gttgcaacag 960

cgggccgcgc ttcagtggcc acggctgcgc gtggagcaac ggccggcctt accgggcctg 1020

gggccggccg tccagatcaa tgaggctttc accgtcagcg tcaccgatgg ccgcacggcg 1080

agccagttgg cggagctgac ggcggcggat gcctttgtgg tcgatgtcgc cctgaactac 1140

gccgacacgg cgtatctggt ggcagcgcac aaccgccacg cctctgcggc caataaggcg 1200

ctgtttttac gcctgctgca cacggcaatc ccgcaggttg aatttatcaa ggactccccg 1260

gcgctgatcg tcgcccgcgt cctcagcagc ctgatcaacg agtcggtgat catggtggaa 1320

agcggcgtct gcagccggga agacatcgat gtcgccgccg tcgccggcgt taactacgcc 1380

gacggcattt tcggctggct cgataacctg ggggagaaaa acgtcaggac gacgctgagc 1440

aacctggcgc agctgctgca cgcggcacgc tatgcgccgc attacaccct tctacacgcc 1500

gcgcaaccgg cgctgacgac cacgccttaa 1530

<210> 128

<211> 1530

<212> DNA

<213> Serratia marcescens 2880STDY5682892

<400> 128

atggcagaaa gtaatgcggc aattcaatcg gctgcgatta tcggtgcggg aacgatgggc 60

agaggcatcg cttatctttt cgcgcaaaaa ggcattcgca cggtgcttta taatcgcaac 120

ggcaataccc tcaatcaggc tcgcgaatat atcgcgcaag acctgaacaa gaaagtcgaa 180

cagggcaaga tcgcgctgca ggataaaggc gcagtgctgg ccaatctggt gttcacctcg 240

gtgtttgaga ctatcgccga cagcgatctg gtgatagaaa ccatcgccga gcaagagcaa 300

accaaacttg aggtgctggc ggccatcgcc gcggcggtca agcccgacac gctgatcgcc 360

accaatacct cctcactgtc gcttaacaag ctggcaaccg cggtgacgca cagcgagcgg 420

tttatcggtt tgcacttttt caatcccgcg ccgctgatga aactgattga aatcatcccg 480

gcctacttta ccgcacgcgc caccaccgaa cgttgccgtc agctggtagc cgcgttgggg 540

aaacgcgatg tcgtctgcca ggccacgccg gggtttatcg tcaaccgcat ggcccgcccc 600

tactacctgg aagggttccg cctgttggaa gaacacgtgg cgcgcgcgcc gcagatcgac 660

cgcgccctca aggccggcgg gcactttcgc atggggccgc tcgagctgac cgattttatc 720

ggccaggaca tcaactatca ggtcagccgg caaatctggc aggacatgca atacgatccg 780

cgctataccc ccggccatct gcagcgttcg ctggtcgatg ccggtctgtt ggggaaaaag 840

aacggccgct cctattttgc cgccgaagaa aacgccttac cggtaacggc cgccaccgat 900

gcagacgtcg agacgctgcg cgtttacggc gaacaccctt tttttaccct gttgcaacag 960

cgggccgcgc ttcagtggcc acggctgcgc gtggagcaac ggccggcctt accgggcctg 1020

gggccggccg tccagatcaa tgaggctttc accgtcagcg tcaccgatgg ccgcacggcg 1080

agccagttgg cggagctgac ggcggcggat gcctttgtgg tcgatgtcgc cctgaactac 1140

gccgacacgg cgtatctggt ggcagcgcac aaccgccacg cctctgcggc caataaggcg 1200

ctgtttttac gcctgctgca cacggcaatc ccgcaggttg aatttatcaa ggactccccg 1260

gcgctgatcg tcgcccgcgt cctcagcagc ctgatcaacg agtcggtgat catggtggaa 1320

agcggcgtct gcagccggga agacatcgat gtcgccgccg tcgccggcgt taactacgcc 1380

gacggcattt tcggctggct cgatagcctg ggggagaaaa acgtcaggac gacgctgagc 1440

aacctggcgc agctgctgca cgcggcacgc tatgcgccgc attacaccct tctgcacgcc 1500

gcgcaaccgg cgctgacgac cacgccttaa 1530

<210> 129

<211> 1530

<212> DNA

<213> Serratia marcescens SM39

<400> 129

atggcagaaa gtaatgcggc aattcaatcg gctgcgatta tcggcgcggg aacgatgggc 60

agaggcatcg cttatctttt cgcgcaaaaa ggcattcgca cggtgcttta taatcgcaac 120

ggcaataccc tcaatcaggc tcgcgaatat atcgtgcaag acctgaacaa gaaagtcgaa 180

cagggcaaga tcgcgctgca ggataaaggc gcagtgctgg ccaatctggt gttcacctcg 240

gtgtttgaga ctatcgccga cagcgagctg gtgatagaaa ccatcgccga gcaagagcaa 300

accaaacttg aggtgctggc ggccatcgcc gcggcggtca agcccgacac gctgatcgcc 360

accaatacct cctcactgtc gcttaacaag ctggcaaccg cggtgacgca cagcgagcgg 420

tttatcggtt tgcacttttt caatcccgcg ccgctgatga aactgattga aatcatcccg 480

gcctacttta ccgcacgcgc caccaccgaa cgttgccgtc agctggtggc cgcgttgggg 540

aaacgcgatg tcgtctgcca ggccacgccg gggtttatcg tcaaccgcat ggcccgcccc 600

tactacctgg aagggttccg cctgttggaa gaacacgtgg cgcgcgcgcc gcagatcgac 660

cgcgccctca aggccggcgg gcactttcgc atggggccgc tcgagctgac cgattttatc 720

ggccaggaca tcaactatca ggtcagccgg caaatctggc aggacatgca atacgatccg 780

cgctataccc ccggccatct gcagcgttcg ctggtcgatg ccggtctgtt ggggaaaaag 840

aacggccgct cctattttgc cgccgaagaa aacgccttac cggtaacggc cgccaccgat 900

gcagacgtcg agacgctgcg catttacggc gaacaccctt tttttaccct gttgcaacag 960

cgggccgcgc ttcagtggcc acggctgcgc gtggagcaac ggccggcctt accgggcctg 1020

gggccggccg tccagatcaa tgaggctttc accgtcagcg tcaccgatgg ccgcacggcg 1080

agccagttgg cggagctgac ggcggcggat gcctttgtgg tcgatgtcgc cctgaactac 1140

gccgacacgg cgtatctggt ggcagcgcac aaccgccacg cctctgcggc caataaggcg 1200

ctgtttttac gcctgctgca cacggcaatc ccgcaggttg aatttatcaa ggactccccg 1260

gcgctgatcg tcgcccgcgt cctcagcagc ctgatcaacg agtcggtgat catggtggaa 1320

agcggcgtct gcagccggga agacatcgat gtcgccgccg tcgccggcgt taactacgcc 1380

gacggcattt tcggctggct cgataacctg ggggagaaaa acgtcaggac gacgctgagc 1440

aacctggcgc agctgctgca cgcggcacgc tatgcgccgc attacaccct tctacacgcc 1500

gcgcaaccgg cgctgacgac cacgccttaa 1530

<210> 130

<211> 1530

<212> DNA

<213> Serratia marcescens 189

<400> 130

atggcagaaa gtaatgcggc aattcaatcg gctgcgatta tcggcgcggg aacgatgggc 60

agaggcatcg cttatctttt cgcgcaaaaa ggcattcgca cggtgcttta taatcgcaac 120

ggcaataccc tcaatcaggc tcgcgaatat atcgcgcaag acctgaacaa gaaagtcgaa 180

cagggcaaga tcgcgctgca ggataaaggc gcagtgctgg ccaatctggt gttcacctcg 240

gtgtttgaga ctatcgccga cagcgatctg gtgatagaaa ccatcgccga gcaagagcaa 300

accaaacttg aggtgctggc ggccatcgcc gcggcggtca agcccgacac gctgatcgcc 360

accaatacct cctcactgtc gcttaacaag ctggcaaccg cggtgacgca cagcgagcgg 420

tttatcggtt tgcacttttt caatcccgcg ccgctgatga aactgattga aatcatcccg 480

gcctacttta ccgcacgcgc caccaccgaa cgttgccgtc agctggtagc cgcgttgggg 540

aaacgcgatg tcgtctgcca ggccacgccg gggtttatcg tcaaccgcat ggcccgcccc 600

tactacctgg aagggttccg cctgttggaa gaacacgtgg cgcgcgcgcc gcagatcgac 660

cgcgccctca aggccggcgg gcactttcgc atggggccgc tcgagctgac cgattttatc 720

ggccaggaca tcaactatca ggtcagccgg caaatctggc aggacatgca atacgatccg 780

cgctataccc ccggccatct gcagcgttcg ctggtcgatg ccggtctgtt ggggaaaaag 840

aacggccgct cctattttgc cgccgaagaa aacgccttac cggtaacggc cgccaccgat 900

gcagacgtcg agacgctgcg catttacggc gaacaccctt tttttaccct gttgcaacag 960

cgggccgcgc ttcagtggcc acggctgcgc gtggagcaac ggccggcctt accgggcctg 1020

gggccggccg tccagatcaa tgaggctttc accgtcagcg tcaccgatgg ccgcacggcg 1080

agccagttgg cggagctgac ggcggcggat gcctttgtgg tcgatgtcgc cctgaactac 1140