阅读说明：本技术 一种植物蛋白替代肉的加工方法 (Processing method of vegetable protein substituted meat ) 是由 李�赫 刘新旗 曹金诺 陈天鹏 刘晶鑫 于 2020-12-30 设计创作，主要内容包括：本发明属于食品加工领域,提供了一种植物蛋白替代肉的加工方法,包括以下步骤：(1)将植物组织蛋白与液体乳液浸泡并滚揉；(2)通过抽真空的工艺使步骤(1)所得的植物组织蛋白内部填充液体乳液；(3)向步骤(2)所得的植物组织蛋白添加胶体粘合剂并进行滚揉；(4)通过抽真空的工艺使步骤(3)所得的植物组织蛋白表层布满胶体粘合剂；以及(5)将步骤(4)的植物组织蛋白和胶体粘合剂混合物用模具压合成形状并加热熟化定型,从而获得植物蛋白替代肉。由本发明的方法加工而成的植物蛋白替代肉块大、口感多汁细嫩、植物组织蛋纤维中充满脂肪,煎炒时有丰富的油脂。(The invention belongs to the field of food processing, and provides a processing method of vegetable protein substituted meat, which comprises the following steps: (1) soaking plant tissue protein and liquid emulsion and rolling and kneading; (2) filling the plant tissue protein obtained in the step (1) with liquid emulsion by a vacuum-pumping process; (3) adding a colloidal adhesive to the plant tissue protein obtained in the step (2) and rolling and kneading; (4) distributing a colloid adhesive on the surface layer of the plant tissue protein obtained in the step (3) by a vacuumizing process; and (5) pressing the mixture of the plant tissue protein and the colloidal adhesive in the step (4) into a shape by a mould, heating, curing and shaping, thereby obtaining the plant protein substituted meat. The vegetable protein processed by the method of the invention has large substituted meat block, juicy and tender mouthfeel, and the vegetable tissue egg fiber is full of fat and has rich grease when fried.)

1. A processing method of vegetable protein substituted meat is characterized by comprising the following steps:

(1) soaking plant tissue protein and liquid emulsion and rolling and kneading;

(2) filling the plant tissue protein obtained in the step (1) with liquid emulsion by a vacuum-pumping process;

(3) adding a colloidal adhesive to the plant tissue protein obtained in the step (2) and rolling and kneading;

(4) distributing a colloid adhesive on the surface layer of the plant tissue protein obtained in the step (3) by a vacuumizing process; and

(5) and (4) pressing the mixture of the plant tissue protein and the colloidal adhesive in the step (4) into a shape by using a mould, and heating, curing and shaping to obtain the plant protein substituted meat.

2. The method as claimed in claim 1, wherein the vegetable tissue protein is a micro-puffing tissue protein with porous type formed by single-screw or double-screw high-temperature extrusion, and the water content is 5-35%.

3. The method as claimed in claim 1, wherein the liquid emulsion in step (1) is formed by mixing and emulsifying water, soy protein, fat and starch.

4. The processing method of vegetable protein substituted meat as claimed in claim 3, wherein the oil and fat content is 4-10%, the soybean protein content is 4-10%, the moisture content is 75-90%, and the starch content is 2-6% by mass of the liquid emulsion, and preferably the oil and fat can be edible animal oil, edible vegetable oil or edible blend oil, preferably the starch can be one or more of corn starch, tapioca starch, sweet potato starch, potato starch and wheat starch, and further preferably sweet potato starch.

5. The method as claimed in claim 1, wherein the mass ratio of the plant tissue protein to the liquid emulsion is 1:1-1: 2.2.

6. The method for processing vegetable protein-substituted meat as claimed in claim 1, wherein in said step (2), the degree of vacuum is from-80 KPa to-100 KPa.

7. The process of claim 1, wherein said colloidal binder in step (3) is prepared by mixing water, soy protein, polysaccharide, soda, and transglutaminase, wherein said soy protein content is 8-15%, said polysaccharide content is 4-8%, said transglutaminase content is 0.2-1%, said soda content is 0.2-0.6%, based on the mass of said colloidal binder, and wherein the mass ratio of said colloidal binder to said plant tissue protein is preferably 1:0.8-1: 1.5.

8. The method for processing vegetable protein-substituted meat as claimed in claim 1, wherein in said step (4), the degree of vacuum is from-30 KPa to-79 KPa.

9. The method for processing vegetable protein-substituted meat as claimed in claim 1, wherein said heating, cooking and shaping in step (5) is divided into two stages, the first stage is an initial cooking stage, the heating temperature is 40-65 ℃, and the heating time is 1-4 h; the second stage is a curing and shaping stage, the heating temperature is 80-99 ℃, and the heating time is 1-4 h.

10. A vegetable protein-substituted meat produced by the method of any one of claims 1 to 9.

Technical Field

The invention relates to the field of food processing, in particular to a processing method of vegetable protein substituted meat.

Background

The increased nutritional demand for proteins has led in recent years to a continued increase in the consumption of vegetable proteins in food products, and the importance of legume and oilseed proteins in the production of functional food products has increased. The plant tissue protein is a protein product with a specific shape and a tissue state formed by crushing, humidifying and mixing materials and then putting the materials in a die head with a specified shape under the action of high temperature and high pressure. The plant tissue protein has the advantages of good animal protein fibrous structure, low allergen, low anti-nutritional factor, high digestion utilization rate, reduced unpleasant odor, high meat replacement cost performance and the like.

The plant tissue protein is porous micro-swelling plant protein, is in the form of particles, strips, sheets and spheres, is not suitable for direct eating or cooking, and is prepared into plant protein substitute meat, called plant meat for short, through the processes of blending, shaping, curing and the like. Almost all foreign vegetable protein meat-substitute enterprises develop products according to the mainstream dietary habits of the European and American population, and the most common product forms are hamburgers, sausages, meat pies, meat paste and the like which accord with the European and American fast food mode. But this does not conform to the dietary habits of Chinese consumers. The Chinese consumers have the requirement of creating color, aroma and shape similar to real meat, especially the requirement of large vegetable meat, and the Chinese consumers are suitable for the frying and frying cooking modes of Chinese dishes through dicing and slicing. Therefore, the development of vegetable meat protein replacing meat in China is based on rich dietary culture and food material resources.

At present, the main problem with vegetable protein replacement meat products is that large chunks of vegetable protein replace meat (typically greater than 4cm in volume)³) Because the fat content is low, the dried tofu has no juicy taste, is dry and astringent, tastes like dried tofu after frying, loses the fiber feeling of meat, and grease can not enter and can not taste after frying. Secondly, the adhesion of the large vegetable protein substituted meat is poor, and the large vegetable protein substituted meat is easy to break after slicing; unlike hamburger patties and dumpling fillings, the adhesion of large vegetable meat protein-substitute meats is not simply a coherent bond, except that the molecular level of vegetable protein is required to be intramolecular and intermolecular covalentAnd (3) crosslinking, namely the colloid adhesive on the surface structure can be embedded into pores of the plant tissue protein, so that the adjacent small pieces of plant tissue protein are bonded through multi-point bridging, and the bonding strength is enhanced.

Therefore, the processing method of vegetable protein substituted meat, in particular large vegetable protein substituted meat, which has juicy and tender mouthfeel, is full of fat in vegetable tissue egg fiber, has good adhesiveness and is not easy to break after being sliced, is urgently needed in the field.

Disclosure of Invention

In view of the above, the present invention aims to provide a processing method of vegetable protein-substituted meat, which can solve the problems of dry and astringent vegetable protein-substituted meat, poor taste, poor adhesiveness, and easy breakage after slicing, etc. the vegetable protein-substituted meat processed by the method has large meat mass, juicy and tender mouthfeel, and the vegetable tissue egg fiber is filled with fat and has firm structure adhesion.

The object of the present invention and the solution of the technical problem are achieved by the following technical means.

In one aspect, the invention provides a processing method of vegetable protein substituted meat, which comprises the following steps:

(1) soaking plant tissue protein and liquid emulsion and rolling and kneading;

(2) filling the plant tissue protein obtained in the step (1) with liquid emulsion by a vacuum-pumping process;

(3) adding a colloidal adhesive to the plant tissue protein obtained in the step (2) and rolling and kneading;

(4) distributing a colloid adhesive on the surface layer of the plant tissue protein obtained in the step (3) by a vacuumizing process; and

(5) and (4) pressing the mixture of the plant tissue protein and the colloidal adhesive in the step (4) into a shape by using a mould and heating, curing and shaping to form the plant protein substituted meat.

In another aspect, the present invention provides a vegetable protein-substituted meat prepared by the above method, which may be a large chunk of vegetable protein-substituted meat and may have a volume of at least 4cm³Can cook according to Chinese foodIs habitually used for dicing and slicing, has rich grease during frying and frying, and has juicy, greasy feeling and elasticity during chewing.

Compared with the prior art, the invention has obvious beneficial effects. According to the technical scheme, the vegetable tissue protein is soaked and rolled by the liquid emulsion, so that the emulsion containing the fat is filled in the vegetable tissue protein, and the vegetable tissue protein substitutes meat and contains abundant fat, and the fat flavor is good; through vacuum treatment, the emulsion is positioned in the middle and at the bottom of the porous tissue protein pores, the colloid adhesive is positioned at the upper part of the tissue protein pores, the colloid adhesive can form a thermally irreversible colloid at the temperature of more than 80 ℃, and the emulsion can be locked in the pores of small tissue proteins, so that the vegetable protein substituted meat has succulent feeling; through vacuum treatment, glutamine transaminase in the colloidal adhesive can enter pores and surfaces of the tissue protein, and covalent crosslinking can be generated in molecules and among molecules of the plant tissue protein and the soybean protein at a molecular level; after vacuum treatment, the colloidal adhesive on the characterization structure of the plant tissue protein can be embedded into the upper parts of plant tissue protein pores, multi-point bridging bonding is realized between adjacent small pieces of plant tissue protein, the bonding strength is strengthened, and large pieces of plant protein substitute meat is obtained.

Detailed Description

The technical solutions of the present invention will be clearly and completely described below in connection with specific embodiments, but it should be understood by those skilled in the art that the embodiments described below are only for illustrating the present invention and should not be construed as limiting the scope of the present invention. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.

Plant tissue protein

The plant tissue protein is porous micro-swelling plant tissue protein formed by single-screw or double-screw high-temperature extrusion, and the water content of the plant tissue protein can be 5-35%. In the embodiment of the invention, the plant tissue protein is usually prepared by extruding one or more of soybean protein, pea protein and wheat protein through single screw or double screw at high temperature.

In particular embodiments, the moisture content of the plant tissue protein may be 6-34%, 7-33%, 8-32%, 9-31%, 10-30%, 11-29%, 12-28%, 13-27%, 14-26%, 15-25%, 16-24%, 17-23%, 18-22%, 19-21%, e.g., 5%, 6%, 6.5%, 7%, 7.5%, 8%, 5.5%, 9%, 9.5%, 10%, 10.5%, 11%, 11.5%, 12%, 12.5%, 13%, 13.5%, 14%, 14.5%, 15%, 15.5%, 16%, 16.5%, 17%, 17.5%, 18%, 18.5%, 19%, 20.5%, 21%, 21.5%, 22%, 22.5%, 23%, 23.5%, 24%, 24.5%, 25%, 25.5%, 26%, 26.5%, 27%, 27.5%, 28%, or more, 28.5%, 29%, 29.5%, 30%, 31%, 32%, 33%, 34%, 35%.

If the moisture content of the porous micro-swelling plant tissue protein is more than 35 percent, the taste is soft and tender, the moisture content is higher, the milk absorbing capacity is weak, and the milk is difficult to transfer to the inside even by a vacuum process.

The non-swelling plant tissue protein has a compact structure, although the non-swelling plant tissue protein has water absorption capacity, water in the emulsion can partially migrate into the tissue protein, the compact tissue protein surface can form a membrane filtration effect, and solid matters in the emulsion can be trapped on the surface of the tissue protein, so that juicy mouthfeel cannot be formed.

Liquid emulsions

In an embodiment of the present invention, the liquid emulsion in step (1) may be formed by mixing and emulsifying water, soy protein, fat and starch.

In the embodiment of the present invention, the oil is well known to those skilled in the art, and may be common edible oil, preferably edible oil such as edible animal oil, edible vegetable oil or edible blend oil, the animal oil may be beef tallow, mutton tallow, lard or the like, and the vegetable oil may be soybean oil, peanut oil, rapeseed oil, sunflower seed oil, corn germ oil, cotton seed oil, sesame oil, olive oil or vegetable blend oil or the like, but is preferably vegetable oil or vegetable blend oil. In embodiments of the invention, the amount of oil may be 4-10%, e.g., 4%, 4.5%, 5%, 5.5%, 6%, 6.5%, 7%, 7.5%, 8%, 8.5%, 9%, 9.5%, 10%, and amounts between any two of the foregoing, e.g., 4.1%, 4.2%, 5.1%, 5.3%, 6.1%, 6.3%, etc., by weight of the liquid emulsion.

In embodiments of the invention, the soy protein may be present in an amount of 4-10%, e.g., 4%, 4.5%, 5%, 5.5%, 6%, 6.5%, 7%, 7.5%, 8%, 8.5%, 9%, 9.5%, 10%, and amounts between any two of the foregoing, e.g., 4.1%, 4.2%, 5.1%, 5.3%, 6.1%, 6.3%, etc., by weight of the liquid emulsion.

In an embodiment of the present invention, the starch may be one or more of corn starch, tapioca starch, sweet potato starch, wheat starch, and the like. In a preferred embodiment of the invention, the starch is sweet potato starch. In embodiments of the invention, the starch content may be from 2 to 6% by weight of the liquid emulsion. In particular embodiments of the present invention, the starch content may be 2%, 2.5%, 3.0%, 3.5%, 4.0%, 4.5%, 5.0%, 5.5%, 6.0%, and values between any two values, such as 2.1%, 2.2%, 2.3%, 3.1%, 3.2%, 3.3%, 3.4%, etc., by weight of the liquid emulsion.

In embodiments of the invention, the moisture content may be from 75 to 90% by weight of the liquid emulsion. In particular embodiments of the invention, the moisture content may be 75.5%, 76%, 76.5%, 77%, 77.5%, 78%, 78.5%, 79%, 79.5%, 80%, 80.5%, 81%, 81.5%, 82%, 82.5%, 83%, 83.5%, 84%, 84.5%, 85%, 85.5%, 86%, 82.5%, 87%, 87.5%, 88%, 88.5%, 90%.

In step (1) of the present invention, the mass ratio of the plant tissue protein to the liquid emulsion may be 1:1 to 1: 2.2. In particular embodiments of the invention, the mass ratio of plant tissue protein to liquid emulsion may be 1:1, 1:1.1, 1:2, 1:1.3, 1:4, 1:1.6, 1:6, 1:1.7, 1:8, 1:1.9, 1:2.0, 1:2.1, 1:2.2, and any ratio therebetween.

The liquid emulsion has low solid content and good fluidity in the process of implementing the step (1) and the step (2), so the emulsion can be migrated into the plant tissue protein through the liquid absorption capacity of the plant tissue protein and a vacuum process, and the oleoresin gel can be formed in the pores of the plant tissue protein after the heating in the step (5), thereby providing juicy mouthfeel.

Colloidal binder

In an embodiment of the present invention, the colloidal binder in step (3) is prepared by mixing water, soy protein, polysaccharide, soda, glutamine transaminase.

In an embodiment of the invention, the soy protein content may be 8-15% by mass of the colloidal binder. In particular embodiments of the invention, the soy protein content may be 8%, 8.5%, 9%, 9.5%, 10%, 10.5%, 11%, 11.5%, 12%, 12.5%, 13%, 13.5%, 14%, 14.5%, 15% by mass of the colloidal binder.

As is well known to those skilled in the art, amidotransaminase is a monomeric protein with a molecular weight of about 38000 consisting of 331 amino groups and having an active center, which can catalyze the covalent intramolecular and intermolecular crosslinking of soybean protein, thereby improving the structure and function of the protein. In an embodiment of the invention, the transglutaminase content may be 0.2-1% based on the mass of the colloidal binder. In a particular embodiment of the invention, the transglutaminase content may be 0.2%, 0.25%, 0.3%, 0.35%, 0.4%, 0.45%, 0.5%, 0.55%, 0.6%, 0.65%, 0.7%, 0.75%, 0.8%, 0.85%, 0.9%, 0.95%, 1% by mass of the colloidal binder.

The soda can improve the solubility of the soybean protein and promote the cross-linking reaction between the soybean protein molecules. In an embodiment of the invention, the soda content may be 0.2-0.6% by mass of the colloidal binder. In particular embodiments of the invention, the soda content may be 0.2%, 0.25%, 0.3%, 0.35%, 0.4%, 0.45%, 0.5%, 0.55%, 0.6% by mass of the colloidal binder.

In an embodiment of the invention, the polysaccharide may be present in an amount of 4-8% by mass of the colloidal binder. In particular embodiments of the invention, the polysaccharide may be present in an amount of 4%, 4.5%, 5%, 5.5%, 6%, 6.5%, 7%, 7.5%, 8% by mass of the colloidal binder.

In an embodiment of the invention, the polysaccharide may be a polysaccharide commonly used in the food processing industry, preferably curdlan and glucomannan. The polysaccharide can form a thermal gel, especially a polysaccharide based on a curdlan and glucomannan. In the heating, curing and shaping stage of the step (5), the polysaccharide and the crosslinked soybean protein and the protein molecules on the surface of the plant tissue protein can generate a synergistic effect, so that the strength of the colloidal adhesive is improved.

In an embodiment of the invention, the mass ratio of colloidal binder to plant tissue protein (i.e. the plant protein described in step 1, excluding the mass of the liquid emulsion) may be from 1:0.8 to 1: 1.5. In a particular embodiment of the invention, the mass of the colloidal adhesive to the plant tissue protein may be 1:0.8, 1:0.9, 1:1.0, 1:1.1, 1:1.2, 1:1.3, 1:1.4, 1:1.5 based on the mass of the colloidal adhesive.

Vacuumizing process

The evacuation in step (2) and step (4) of the present invention is well known to those skilled in the art, and may be carried out by a horizontal rotary vacuum tank or a stirring type vacuum tank, and the vacuum is released to return to normal pressure immediately after the degree of vacuum defined in step (2) and step (4) of the present invention is reached.

In the embodiment of the present invention, in the step (2), the degree of vacuum after the vacuuming process may be-80 KPa to-100 KPa. In a specific embodiment of the present invention, in the step (2), the degree of vacuum after the evacuation process may be, for example, -80KPa, -80.5KPa, -81KPa, -81.5KPa, -82KPa, -82.5KPa, -83KPa, -83.5KPa, -84KPa, -84.5KPa, -85KPa, -85.5KPa, 86KPa, -86.5KPa, -87KPa, -87.5KPa, -88KPa, -88.5KPa, -89KPa, -89.5KPa, 90KPa, -90.5KPa, 91KPa, -91.5KPa, -92KPa, -92.5KPa, -93KPa, -93.5KPa, -94KPa, -94.5KPa, 95 a, -95.5KPa, -96KPa, -96.5KPa, -97.5KPa, 98 a, -98.5KPa, -99.99.5 KPa, -99.5KPa, -83.5KPa, -100 KPa.

In the embodiment of the present invention, in the step (4), the degree of vacuum after the vacuuming process may be-30 KPa to-79 KPa. In the embodiment of the present invention, in the step (4), the degree of vacuum after the vacuuming process may be, for example, -35KPa to-74 KPa, -40KPa to-69 KPa, -45KPa to-64 KPa, -50KPa to-59 KPa, and values between the above-mentioned ranges, for example, -30KPa, -31KPa, -32KPa, -33KPa, -34KPa, -36KPa, -37KPa, -38KPa, -39KPa, etc.

In the embodiment of the present invention, the degree of vacuum in step (4) is preferably lower than that in step (2). If the vacuum degree in the step (4) is higher than that in the step (2), the liquid emulsion which enters the porous tissue protein can be sucked out in a large amount, the colloidal adhesive is greatly diluted, the adhesive capacity is reduced, and the succulent feeling of the vegetable protein replacing meat is reduced due to excessive loss of the liquid emulsion in pores of the porous tissue protein. In addition, the colloidal binder is highly solid and viscous, and under the vacuum in step (4), a small amount of the colloidal binder can enter the top of the tissue protein pores without the colloidal binder displacing the liquid emulsion that has entered the porous tissue protein. Only under the vacuum degree of the step (2) and the step (4) of the invention, the inside of the plant tissue protein pore is liquid emulsion, and the top of the pore and the surface of the plant tissue protein are the characteristics of the colloidal adhesive. The liquid emulsion can be sealed inside the plant tissue protein pores by a colloidal adhesive. After the heating, curing and shaping in the step (5), stable gel can be formed by both the liquid emulsion and the colloidal adhesive.

Heating, curing and shaping

In the embodiment of the present invention, the heat curing and shaping step (5) is divided into two stages, the first stage is a primary curing stage, the mixture of the plant tissue protein, the liquid emulsion and the colloidal binder in the mold is heated to 40-65 ℃ for 1-4 h. This stage allows the amidotransaminase to function effectively, catalyzing the soy protein to undergo intramolecular and intermolecular covalent cross-linking. The second stage is a curing and shaping stage, the temperature of the mixture of the plant tissue egg, the liquid emulsion and the colloid adhesive in the mold is 80-99 ℃ by heating, and the heating time is 1-4 h. This stage allows the protein and polysaccharide to form a stable gel. If the temperature of the first stage is too high, the aminoacyl amine transaminase is inactivated, and if the temperature is too low, the effect of the transaminase is poor; if the second temperature does not reach the temperature and time specified in step (5), the protein and polysaccharide will not form a stable gel.

In the embodiment of the present invention, in the step (5), the heating temperature of the first stage may be 40 ℃, 41 ℃, 42 ℃, 43 ℃, 44 ℃, 45 ℃, 46 ℃, 47 ℃, 48 ℃, 49 ℃, 50 ℃, 51 ℃, 52 ℃, 53 ℃, 54 ℃, 55 ℃, 56 ℃, 57 ℃, 58 ℃, 59 ℃, 60 ℃, 61 ℃, 62 ℃, 63 ℃, 64 ℃, 65 ℃ and a temperature between any two of the above values, for example, 40.5 ℃, 41.5 ℃ and the like, and the heating time may be 1 hour, 1.5 hours, 2 hours, 2.5 hours, 3 hours, 3.5 hours.

In an embodiment of the present invention, in step (5), the heating temperature of the second stage may be 80 ℃, 81 ℃, 82 ℃, 83 ℃, 84 ℃, 85 ℃, 86 ℃, 87 ℃, 88 ℃, 89 ℃, 90 ℃, 91 ℃, 92 ℃, 93 ℃, 94 ℃, 95 ℃, 96 ℃, 97 ℃, 98 ℃, 99 ℃, and a temperature between any two of the above values, such as 80.5 ℃, 81.5 ℃, 82.5 ℃, 83.5 ℃, 84.5 ℃, 85.5 ℃, 86.5 ℃, 87.5 ℃, 88.5 ℃, 90.5 ℃, 91.5 ℃, 92.5 ℃, 93.5 ℃, 94.5 ℃, 95.5 ℃, 96.5 ℃, 97.5 ℃, 98.5 ℃ and the like.

Another aspect of the present invention provides vegetable protein-substituted meat prepared by the above method, which may be large vegetable protein-substituted meat chunks having a volume of at least 4cm³The vegetable protein substitute meat has juicy and tender mouthfeel, the pores of the vegetable tissue protein are full of fat, and the vegetable protein substitute meat is rich in grease during frying and has juicy, greasy and elastic feeling during chewing.

In an embodiment of the invention, the volume of the vegetable protein substitute meat may be at least 5cm³At least 10cm³At least 50cm³At least 100cm³At least 150cm³At least 200cm³、250cm³At least 300cm³At least 350cm³At least 400cm³At least 450cm³At least 500cm³At least 550cm³、600cm³At least 650cm³At least 700cm³At least 750cm³At least 800cm³、850cm³At least 900cm³At least 950cm³At least 1000cm³At least 1100cm³At least 1200cm³At least 1300cm³、1400cm³At least 1500cm³And the like.

Preferred embodiments of the present invention will be described in detail with reference to the following examples. It is to be understood that the following examples are given for illustrative purposes only and are not intended to limit the scope of the present invention. Various modifications and substitutions may be made by those skilled in the art without departing from the spirit and scope of the invention, and all such modifications and substitutions are intended to be within the scope of the claims.

The experimental procedures used in the following examples are all conventional procedures unless otherwise specified. Materials, reagents and the like used in the following examples are commercially available unless otherwise specified. For example, the plant tissue protein used in the following examples may be a porous type micro-puffing plant tissue protein manufactured by Shandong Gulin food technology Co., Ltd, and the vacuum tank may be a horizontal type rotary vacuum tank manufactured by City lucky machines Ltd, but is not limited thereto.

Example 1

Soaking 100kg of plant tissue protein with water content of 35% and 100kg of liquid emulsion, and rolling and kneading; the liquid emulsion is formed by mixing and emulsifying 75% of water, 10% of soybean protein, 10% of vegetable blend oil and 5% of sweet potato starch. And vacuumizing in a horizontal rotary vacuum tank at-80 KPa, and recovering to normal pressure to fill the liquid emulsion in the plant tissue protein. Adding 150kg of colloid adhesive for rolling and kneading; the colloid adhesive is prepared from 8% of soybean protein, 4% of coagulated polysaccharide,0.2% glutamine transaminase, 0.2% soda and 87.6% water. And (3) vacuumizing in a horizontal rotary vacuum tank at-79 KPa, and then recovering to normal pressure, so that the tops of plant tissue protein pores and the surfaces of the tissue proteins are filled with colloid adhesives. Pressing with a rectangular stainless steel mold of 20cm x10cm x10cm, heating at 40 deg.C for 4 hr, heating at 80 deg.C for 4 hr for shaping, and making into at least 1000cm³The large pieces of vegetable protein replace meat.

Example 2

Soaking 100kg of plant tissue protein with water content of 5% and 220kg of liquid emulsion, and rolling and kneading; the liquid emulsion is formed by mixing and emulsifying 90% of water, 4% of soybean protein, 4% of vegetable blend oil and 2% of corn starch. And (3) vacuumizing in a horizontal rotary vacuum tank at-100 KPa, and then recovering to normal pressure, so that the liquid emulsion is filled in the plant tissue protein. Adding 80kg of colloid adhesive for rolling and kneading; the colloidal adhesive is prepared by mixing 15% of soybean protein, 8% of glucomannan, 1% of glutamine transaminase, 0.6% of soda and water. And vacuumizing in a horizontal rotary vacuum tank at-30 KPa, and recovering to normal pressure to fill the colloidal adhesive on the tops of plant tissue protein pores and the surfaces of the tissue proteins. Pressing with 4cm x2cm x1cm rectangular stainless steel mold, heating at 65 deg.C for 1 hr, heating at 99 deg.C for 1 hr for shaping, and making into at least 4cm³The large pieces of vegetable protein replace meat.

Example 3

Soaking 100kg of plant tissue protein with water content of 10% and 200kg of liquid emulsion, and rolling and kneading; the liquid emulsion is formed by mixing and emulsifying 84% of water, 5% of soybean protein, 5% of vegetable blend oil and 6% of corn starch. And (3) vacuumizing in a horizontal rotary vacuum tank at-100 KPa, and then recovering to normal pressure, so that the liquid emulsion is filled in the plant tissue protein. Adding 100kg of colloid adhesive for rolling and kneading; the colloidal adhesive is prepared by mixing 10% of soybean protein, 3% of glucomannan, 2% of curdlan, 2% of polyglucose, 0.5% of glutamine transaminase, 0.6% of soda and 81.9% of water. And vacuumizing in a horizontal rotary vacuum tank at-50 KPa, and recovering to normal pressure to fill the colloidal adhesive on the tops of plant tissue protein pores and the surfaces of the tissue proteins. By passingPressing with a rectangular stainless steel mold of 20cm x10cm x10cm, heating at 65 deg.C for 4 hr, heating at 99 deg.C for 4 hr for shaping, and making into a product with a volume of at least 1000cm³The large pieces of vegetable protein replace meat.

Example 4

Soaking 100kg of plant tissue protein with water content of 35% and 100kg of liquid emulsion, and rolling and kneading; the liquid emulsion is formed by mixing and emulsifying 84% of water, 10% of soybean protein, 10% of sunflower seed oil and 2% of tapioca starch. And vacuumizing in a horizontal rotary vacuum tank at-80 KPa, and recovering to normal pressure to fill the liquid emulsion in the plant tissue protein. Adding 150kg of colloid adhesive for rolling and kneading; the colloidal binder is prepared by mixing 8% of soybean protein, 2% of glucomannan, 2% of curdlan, 0.2% of glutamine transaminase, 0.5% of soda and 87.3% of water. And (3) vacuumizing in a horizontal rotary vacuum tank at-79 KPa, and then recovering to normal pressure, so that the tops of plant tissue protein pores and the surfaces of the tissue proteins are filled with colloid adhesives. Pressing with a rectangular stainless steel mold of 20cm x10cm x10cm, heating at 40 deg.C for 1 hr, heating at 80 deg.C for 1 hr for shaping, and making into at least 1000cm³The large pieces of vegetable protein replace meat.

Example 5

Soaking 100kg of plant tissue protein with water content of 5% and 200kg of liquid emulsion, and rolling and kneading; the liquid emulsion is formed by mixing and emulsifying 90% of water, 4% of soybean protein, 4% of grease and 2% of potato and cassava mixed starch. And vacuumizing in a horizontal rotary vacuum tank at-80 KPa, and recovering to normal pressure to fill the liquid emulsion in the plant tissue protein. Adding 80kg of colloid adhesive for rolling and kneading; the colloidal binder is prepared by mixing 8% of soybean protein, 1% of glucomannan, 3% of curdlan, 0.5% of glutamine transaminase, 0.6% of soda and 86.9% of water. And vacuumizing in a horizontal rotary vacuum tank at-50 KPa, and recovering to normal pressure to fill the colloidal adhesive on the tops of plant tissue protein pores and the surfaces of the tissue proteins. Pressing with a rectangular stainless steel mold of 20cm x10cm x10cm, heating at 40 deg.C for 4 hr, heating at 99 deg.C for 1 hr for shaping, and making into at least 1000cm³The large pieces of vegetable protein replace meat.

Comparative example 1

Soaking 100kg of plant tissue protein with water content of 5% and 220kg of liquid emulsion, and rolling and kneading; the liquid emulsion is formed by mixing and emulsifying 90% of water, 4% of soybean protein, 4% of grease and 2% of corn starch. -80KPa vacuum to fill the inside of the plant tissue protein with liquid emulsion. Adding 80kg of colloid adhesive for rolling and kneading; the colloidal adhesive is prepared by mixing 15% of soybean protein, 1% of glutamine transaminase and water. Pressing with a mold, heating at 65 deg.C for 1 hr, heating at 99 deg.C for 1 hr for shaping, and making into final product with volume of at least 1000cm³The large pieces of vegetable protein replace meat.

Comparative example 2

Soaking 100kg of plant tissue protein with water content of 35% and 100kg of liquid emulsion, and rolling and kneading; the liquid emulsion is formed by mixing and emulsifying 75% of water, 10% of soybean protein, 10% of grease and 5% of tapioca starch. Adding 150kg of colloid adhesive for rolling and kneading; the colloidal binder is prepared by mixing 8% of soybean protein, 4% of curdlan, 0.2% of glutamine transaminase, 0.2% of soda and 87.6% of water. Pressing with a mold, heating at 40 deg.C for 4 hr, heating at 80 deg.C for 4 hr for shaping, and making into final product with volume of at least 4cm³The large pieces of vegetable protein replace meat.

Comparative example 3

Soaking 100kg of plant tissue protein with water content of 5% and 300kg of liquid emulsion, and rolling and kneading; the liquid emulsion is prepared from 80% of water, 11% of soybean protein, 4% of glucomannan, 4% of oil and fat, 2% of potato starch, 0.5% of glutamine transaminase and 0.5% of soda through mixing and emulsifying. -90KPa vacuum tumbling. Pressing with a mold, heating at 60 deg.C for 2 hr, heating at 99 deg.C for 2 hr for shaping, and making into final product with volume of at least 1000cm³The large pieces of vegetable protein replace meat.

Comparative example

In comparative examples, juicy feeling, greasy feeling, elasticity, cohesiveness, chewiness, and other properties of the vegetable protein-substituted meat prepared in examples 1 to 5 and comparative examples 1 to 3 were tested and compared.

Elasticity: elasticity represents the ability of an object to deform under the action of an external force and to recover to the original state after the external force is removed.

Cohesion: cohesiveness reflects the property of food to resist damage and to tightly bind when chewed, leaving the food intact.

Chewiness: for describing the solid state test specimens, the values are expressed as the product of the tackiness and the elasticity.

The experimental method comprises the following steps: the vegetable protein substitute meat prepared in examples 1-5 and comparative examples 1-3 was cut into cubes of 1cm x1cm x1cm, and the samples were examined 10 times (n ═ 10) repeatedly using a Brookfield CT3 texture analyzer, compression set (compression distance/sample height) 20%, probe test rate 0.5mm/s, trigger point load 2g, standard probe TA 10.

The test results are shown in tables 1 and 2 below.

TABLE 1 taste of vegetable protein-substituted meat

	Juicy feeling	Feeling of fat	Elasticity
				Example 1	Succulent when chewing	Has a greasy feeling	Has elasticity
Example 2	Succulent when chewing	Has a greasy feeling	Has elasticity
				Example 3	Succulent when chewing	Has a greasy feeling	Has elasticity
Example 4	Succulent when chewing	Has a greasy feeling	Has elasticity
				Example 5	Succulent when chewing	Has a greasy feeling	Has elasticity
Comparative example 1	Succulent when chewing	The grease attached to the surface is not uniform but has no grease feeling	Loose, inelastic
				Comparative example 2	No succulence when chewing	The grease attached to the surface is not uniform but has no grease feeling	Loose, inelastic
Comparative example 3	No succulence when chewing	The grease attached to the surface is not uniform but has no grease feeling	Loose, inelastic

TABLE 2 binding Effect of vegetable protein-substituted meat

	Elasticity mm	Cohesion property	Chewiness mJ
				Example 1	6.15±0.62	0.75±0.03	42.0±5.5
Example 2	5.87±0.66	0.76±0.04	37.0±4.1
				Example 3	5.77±0.32	0.78±0.08	39.3±3.5
Example 4	5.13±0.34	0.76±0.02	29.0±0.8
				Example 5	5.99±0.22	0.76±0.02	35.5±1.2
Comparative example 1	4.51±0.40	0.67±0.03	20.6±3.3
				Comparative example 2	2.55±0.14	0.59±0.04	15.5±2.2
Comparative example 3	2.37±0.07	0.63±0.03	10.9±0.9

From the data in the table, we can see that the vegetable protein-substituted meat prepared in examples 1 to 5 is juicy, greasy, and elastic when chewed, as compared to the comparative example. From the texture data, the indexes of elasticity (mm), cohesiveness and chewiness (mJ) in Table 2 indicate that the bulk vegetable protein-substituted meat prepared in examples 1-5 of the present application has better cohesiveness, better elasticity and better chewiness than the vegetable protein-substituted meat of comparative examples 1-3.

Without being limited by theory, the elastic (mm) and chewable (mJ) effects of the vegetable protein replacement meat in examples 1-5 are primarily such that the processing method of the present invention results in a very good filling of the liquid emulsion in the vegetable tissue protein. Only under the vacuum degree implemented in the step (2) and the step (4) of the invention, the inside of the tissue protein pores is liquid emulsion, and the top of the pores and the surface of the tissue protein are the characteristics of the colloidal adhesive. The liquid emulsion can be sealed inside the plant tissue protein pores by a colloidal adhesive. In addition, the liquid emulsion has low concentration, good fluidity and easy filling, and can form stable gel after the curing and shaping of the second stage of the heating curing and shaping process in the step (5).

Without being limited by theory, the cohesiveness of examples 1-5 is due primarily to the excellent adhesive effect of the colloidal adhesive. In the method of the invention, the heating, curing and shaping process in the step (5) is divided into two sections, wherein the first section is a primary curing stage, the temperature of the mixture of the plant tissue protein, the liquid emulsion and the colloidal adhesive in the mold is controlled to be 40-65 ℃ by heating, and the heating time is 1-4 h. The heating stage enables the amidotransaminase to function effectively, catalyzing the soybean protein to generate covalent intra-molecular and intermolecular cross-linking. The second stage is a curing and shaping stage, the mixture of the plant tissue protein, the liquid emulsion and the colloid adhesive in the mold is heated to 80-99 ℃ for 1-4 h. This heating stage allows the protein and polysaccharide to form a stable gel. If the temperature of the first stage is too high, the aminoacyl-amine transaminase is inactivated, and if the temperature is too low, the effect of the transaminase is poor; if the second temperature does not reach the temperature and time specified in step (5), the protein and polysaccharide are not able to form a stable gel. In addition, the colloidal adhesive not only adheres to the surfaces of the small pieces of plant tissue protein, but also can be embedded into the tops of plant tissue protein pores under the vacuum degree in the step (4), so that multi-point bridging bonding is realized between the adjacent small pieces of plant tissue protein, the bonding strength is strengthened, the volume of the plant protein substituted meat is increased, and the large pieces of plant protein substituted meat is obtained.