阅读说明：本技术 火炭母胶囊治疗和／或预防急性肝损伤的应用 (Application of fire charcoal mother capsule in treatment and/or prevention of acute liver injury ) 是由 朱华 许立拔 谢凤凤 董雪静 苏健 王颖 韦安达 于 2021-02-05 设计创作，主要内容包括：本发明涉及药物应用领域,特别是火炭母胶囊治疗和/或预防急性肝损伤的应用。本发明所述火炭母胶囊对CCl4所致大鼠急性肝损伤有良好的治疗作用,可明显改善肝功能、氧化应激指标和细胞炎症因子水平,可明显减轻CCl4所致肝组织病理性损伤,有明显的护肝作用。(The invention relates to the field of medicine application, in particular to application of a fire charcoal mother capsule in treating and/or preventing acute liver injury. The Chinese knotweed capsule provided by the invention has a good treatment effect on acute liver injury of a rat caused by CCl4, can obviously improve liver function, oxidative stress indexes and levels of cell inflammatory factors, can obviously alleviate pathological liver tissue injury caused by CCl4, and has an obvious liver protection effect.)

1. Application of the fire charcoal mother capsule in treating and/or preventing acute liver injury.

2. The use according to claim 1, wherein the difference between the weight and the weight gain of the rats of the experimental animals does not have significance (P > 0.05) in each dose group of the Chinese knotweed capsules, and the difference has no obvious effect.

3. The application of claim 1, wherein the dose groups of the Chinese knotweed herb capsule have obvious improvement effect on the weight and coefficient of the liver of the rat (P < 0.05 or P < 0.01).

4. The use of claim 1, wherein the level of ALT, AST, TBIL, GLOB and GGT in rat liver function index is decreased and the level of ALB, TP and A/G is increased in each dose group of the Chinese knotweed herb capsule, wherein the level of ALT, GLOB, ALT, AST, TBIL, GLOB and GGT in low dose group, middle dose group and high dose group is significantly decreased (P < 0.05 or P < 0.01), and the level of ALB, A/G and TP in middle dose group is significantly increased (P < 0.05 or P < 0.01), which has obvious improvement effect.

5. The use of claim 1, wherein the serum, liver SOD and liver CAT levels of rats in each dose group of the Chinese knotweed capsule are increased, and the serum, liver MDA and serum NO levels are decreased, wherein the low dose liver CAT and GSH, the medium and high dose liver SOD and CAT and GSH are significantly increased (P < 0.05 or P < 0.01), and the low dose serum MDA, medium and high dose serum MDA and serum NO levels are significantly decreased (P < 0.05 or P < 0.01), which has significant improvement effect on oxidative stress indexes of rat serum and liver.

6. The use of claim 1, wherein the serum, liver tissue IL-1 β, IL-6 and TNF- α levels of rats in each dose group of the kaempferia galanga capsules are all reduced, wherein the serum IL-6, liver tissue TNF- α levels in the low dose group and the serum, liver tissue IL-1 β, IL-6 and TNF- α levels in the medium and high dose groups are significantly reduced (P < 0.05 or P < 0.01), the serum IL-10 levels in each dose group are significantly increased (P < 0.05 or P < 0.01), and the use of the capsules has significant improvement effect on the serum and liver tissue inflammatory factor indexes of rats.

7. The use according to claim 1, wherein the Huocheng mother capsule has a significantly reduced degree of swelling of liver of rat in low dose group, a significantly reduced degree of yellow-white granules of liver tissue, a significantly reduced degree of swelling of liver of rat in medium dose group, no yellow-white granules of liver tissue, but a slightly yellow and light color of liver, a significantly reduced degree of swelling of liver of rat in high dose group, no yellow-white granules of liver tissue, and no significant difference between the color of liver and that of normal group, and can improve pathological change of liver caused by CCl4 to different extent, wherein the effect is optimal with high dose.

Technical Field

The invention relates to the field of medicine application, in particular to application of a fire charcoal mother capsule in treating and/or preventing acute liver injury.

Background

The herb of Chinese knotweed is dry herb of Chinese knotweed (Polygonum chinense L.) of Polygonum of Polygonaceae, is sour, sweet and cold, and has effects of clearing heat and removing dampness, cooling blood and removing toxic substances, benefiting liver and improving eyesight, and dredging channels and promoting blood circulation, and can be used for treating diarrhea, dysentery, cough due to lung heat, sore throat, hepatitis, eczema, otitis media, etc., and is mainly distributed in Fujian, Guangxi, Guangdong, etc. The Guangxi folk Chinese herb and hard bristle Chinese herb (Polygonum chinense L.var. hispidum hook.f.) are used as the Zhuang nationality medicine and the Yao nationality medicine, and are collected and carried by the first volume of Yao nationality medicine quality standard of Guangxi Zhuang nationality autonomous region and the first volume of Guangxi Zhuang nationality medicine quality standard, so that the Chinese herb plays an important role in the Guangxi medical field. The Chinese knotweed herb mainly contains flavonoid, flavonoid glycoside, phenolic acid, steroid and other chemical components, and has the pharmacological effects of resisting hepatitis B virus, protecting liver, resisting inflammation, relieving pain, resisting oxidation, resisting bacteria and the like.

The Baohe soup is prepared from 22kg of Chinese knotweed herb, 22kg of microcos paniculata, 22kg of Siyeteng, 3.4kg of pericarpium citri reticulatae viride, 90kg of folium eriobotryae and 5.0kg of liquorice in a traditional Chinese medicine hospital in the New meeting area of Jiangmen, Guangdong province. Zhang Chenlin, etc. taking five medicinal materials of 100kg of herb of Chinese knotweed, 100kg of Jasminum giraldii, 100kg of plantain, 67kg of ovateleaf holly bark, 33kg of pomegranate rind, etc., extracting, settling in a tank, concentrating to prepare dry paste, crushing and sieving by a 100-mesh sieve to prepare dry paste powder, adding sucrose powder, quickly mixing and granulating, drying, finishing granules, tabletting, sugar coating, and subpackaging to obtain the Fukean tablets. The chuanhonge and the like are also prepared into dispersible tablets by the prescription of herb of Chinese knotweed, Jasminum serrulata, ovateleaf holly bark, plantain herb and pomegranate rind. The first subsidiary hospital of Guangzhou university of traditional Chinese medicine uses 13 medicinal materials of Chinese knotweed herb 210g, radix codonopsitis 105g and the like to prepare thick paste by grinding, extracting, decocting with water and other methods, adding 80g of dextrin, carrying out spray drying to obtain dry extract powder, mixing uniformly, preparing into particles, and filling to obtain the intestinal tract stimulant capsule. Clinical application of the Chinese knotweed herb raw material is mostly a compound preparation, and the use of Chinese knotweed herb single-prescription capsules, particularly the application report of the Chinese knotweed herb capsules for treating and/or preventing acute liver injury is not seen.

Disclosure of Invention

In order to overcome the defects of the prior art, the invention provides a coke master capsule pair CCl₄The method for treating acute liver injury of rats can obviously improve liver function, oxidative stress index and level of cell inflammatory factor, can obviously relieve pathological liver tissue injury caused by CCl4, and has obvious liver protection effect.

The source of the Chinese knotweed herb is dry whole grass of Chinese knotweed herb (Polygonum chinense L.) and Chinese bristlegrass herb (Polygonum chinense L. var. hispidum hook. f.) of Polygonum of Polygonaceae.

The invention is realized by the following technical scheme:

(1) animal model establishment: the equal volume of 0.5% CMC-Na is given according to the body weight, and the stomach is drenched according to 10mL/kg, 1 time every day, and the stomach is drenched continuously for 14 days. Before dosing, Day3, Day6, Day9, Day12, Day13 and Day14 were recorded and weighed 1 time. Day13 was administered, and the normal rats were administered olive oil by intraperitoneal injection at 5mL/kg, and the remaining rats were administered 12% CCl4 olive oil solution by intraperitoneal injection at 5 mL/kg.

(2) Liver function index detection: after the model is made, fasting for 22-24 h without water prohibition, taking about 3mL of blood by using the retroorbital venous plexus after 1h of the last medicine, standing for 1-2 h, 3000rpm, centrifuging for 10min, collecting supernatant, and detecting the ALT, AST, GGT, ALB, TP and TBIL levels of serum for liver function index detection;

(3) detecting oxidative stress indexes: 3mL/kg of 10% chloral hydrate (300mg/kg) was intraperitoneally injected for anesthesia, abdominal aorta was sampled at 3000rpm, centrifuged for 10min, and the supernatant was collected and assayed for IL-1 β, IL-6, IL-10, TNF- α and TGF- β levels using ELISA.

(4) Detection of inflammatory factor indexes: serum samples or liver homogenate supernatants were taken and tested for SOD, MDA, GSH-PX, CAT and NO levels.

(5) Testing rat organ weight and organ coefficient detection: after the blood collection, the rats were sacrificed by cutting off abdominal aorta acute blood loss, the liver, spleen and thymus were rapidly separated, weighed, and organ coefficients (g/100g) were calculated. Organ coefficient 100 × (organ weight/body weight).

(6) Pathological examination of test rats: the liver tissue was observed for changes in color, texture, size, etc., recorded and photographed. Separating the left upper leaf of liver, fixing in 10% formalin solution, replacing fresh 10% formalin solution for 1 time after 6h, fixing for 24h, preparing pathological tablet, staining with hematoxylin-eosin (HE), and observing histopathological change under light microscope.

Compared with the prior art, the invention has the beneficial effects that:

1. the invention provides a Chinese knotweed capsule pair CCl₄In the detected liver function indexes, the level of ALT, AST, TBIL, GLOB and GGT in the low-dose group and the medium-high dose group of the Turkey red capsules is obviously reduced (P is less than 0.05 or P is less than 0.01), the level of ALB, A/G and TP in the medium-dose group is obviously increased (P is less than 0.05 or P is less than 0.01), and the Turkey red capsules have obvious improvement effect on the liver function indexes of rats.

2. The Huochun mother capsule provided by the invention can improve the levels of rat serum, liver tissue SOD and liver tissue CAT, reduce the levels of serum, liver tissue MDA and serum NO, and has an obvious improvement effect on oxidative stress indexes of rat serum and liver tissue.

3. The Huochun mother capsule provided by the invention can reduce the levels of IL-1 beta, IL-6 and TNF-alpha of rat serum and hepatic tissue, and has obvious improvement effect on the indexes of inflammatory factors of rat serum and hepatic tissue.

4. The Huocheng mother capsule provided by the invention can reduce the liver swelling degree of rats, reduce yellow-white particles of liver tissues, and improve the pathological change of liver caused by CCl4 to different degrees, wherein the effect is optimal in high dosage.

Drawings

FIG. 1 shows the fire charcoal female capsule pair CCl₄The pathological change of the liver of the rat with the acute liver injury is visually checked.

FIG. 2 is a diagram of the observation of the fire charcoal female capsule pair CCl by HE dyeing₄The pathological change of the liver of the rat with acute liver injury.

Detailed Description

Example 1 CCl₄Establishment of acute liver injury model

1. Laboratory animal

Provided by the experimental animals of slyke shoda, Hunan, the production license number of the experimental animals: SCXK (xiang) 2019-: 1107272011003888.

2. reagent

The medicine is prepared by mixing the raw materials of 5g, 1g, 8.88g and 1g of crude medicines, wherein the raw materials comprise fire charcoal mother capsules, 4 capsules for 1 time and 3 times for 1 day in a key laboratory of Guangxi Zhuang Yao medicine, and the content of the capsules is brown powder; sodium carboxymethylcellulose, national drug group chemical reagents limited, lot number: 20151215; rat interleukin-1 beta (IL-1 beta), IL-6, IL-10, tumor necrosis factor alpha (TNF-alpha), Nitric Oxide (NO) and Glutathione (GSH) ELISA kit, Jiangsu enzyme immunoassay Co., Ltd., each under the batch number of 202006; superoxide dismutase (SOD), Catalase (CAT) and Malondialdehyde (MDA) kit, Nanjing was established as bioengineering institute, and the batch numbers are 20200923, 20200715 and 20200814 respectively; BCA protein assay kit, Shanghai Beihu Biotech limited, lot number: 20200619, respectively; alanine Aminotransferase (ALT) kit, shanghai kowa bioengineering, ltd, lot number: 20200422, respectively; glutamyl Transferase (GGT) kit, zhongsheng bei zhi biotechnology limited, lot no: 92638110, respectively; total Bilirubin (TBIL) kit, beijing lidman biochemistry gmbh, lot No.: 19112708, respectively; albumin (ALB), Total Protein (TP), and aspartate Aminotransferase (AST) kits, zhejiang elikang biotechnology limited, with lot numbers 200301, 200401, and 200401, respectively; chloral hydrate, national pharmaceutical group chemical reagents ltd, batch No.: 20160225, respectively; carbon tetrachloride (CCl)₄) Shanghai Mielin Biochemical technology, Inc., lot number: c11136534; olive oil, shanghai alatin biochemical science & technology ltd, lot No.: e202108, and the like.

3. Instrument for measuring the position of a moving object

MH-693 electronic balance, MingHei electronic science and technology, Inc., Dongguan; ES 2220M electronic balance, switzerland prisos; 0104 type biosafety independent ventilation cage (IVC cage), shenzhen, hong teng biotechnology limited; 5810R model high speed refrigerated centrifuge, egend, germany; an Epoch2 model microplate reader, Bio-Tek Instruments, USA; TENLIN-C homogenizer, feathering instruments Inc. of Jiangsu; MDF-U74V model-80 deg.C refrigerator, Japan Panasonic corporation; Direct-Q5 UV-R model ultrapure water, Merck Misco, USA; DHG-9240A type forced air drying cabinet, shanghai-chang scientific instruments ltd; 7180 model full-automatic biochemical analyzer, HITACHI, Japan, and the like.

4. Feeding environment

The animal feed is bred in the center barrier environment of the experimental animals of Guangxi Chinese medicine university, meets the SPF level requirement, and the license number of the experimental animals is SYXK (cinnamon) 2019-. A central air conditioner is used, the room temperature is controlled to be 19.0-25.0 ℃, the relative humidity is controlled to be 40.0-70.0%, and the illumination brightness is controlled to be 12h/12 h; the rats are raised in non-toxic, high-pressure resistant, high-temperature resistant and corrosion resistant IVC plastic cages, no more than 6 rats per cage are fed with SPF level rats to maintain the feed, and the rats can freely eat and drink purified water.

5. Method of producing a composite material

Selecting 60 healthy SD rats, and randomly dividing the SD rats into 6 groups according to weight by using an Excel software block layering random grouping method^[1]Each group contains 10 drugs, including normal group, model group, silymarin group (120mg/kg)^[2]And low, medium and high dose groups of the Chinese knotweed capsules (240, 479 and 958 mg/kg). The Chinese knotweed capsule dosage group is used for administering Chinese knotweed alcohol extract liquid medicine with corresponding concentration according to the body weight; silymarin group administered silymarin liquid medicine with corresponding concentration according to body weight; the model group and the normal group are administrated with equal volume of 0.5% CMC-Na according to the weight, and the stomach is drenched according to 10mL/kg for 1 time each day and continuously drenched for 14 days. Recording before administration, Day₃、Day₆、Day₉、Day₁₂、Day₁₃And Day₁₄Weighing 1 time, and administering Day₁₃The rats in the normal group were administered olive oil by intraperitoneal injection at 5mL/kg, and the remaining rats were administered 12% CCl by intraperitoneal injection at 5mL/kg₄Olive oil liquid medicine^[2]。

5.1 blood sampling

After the model is made, fasting is not forbidden for 22-24 h, after 1h of the last medicine, 3mL of blood is collected by using the retroorbital venous plexus, after the blood is kept stand for 1-2 h, 3000rpm is carried out, centrifugation is carried out for 10min, and supernatant is collected for liver function index detection; injecting 10% chloral hydrate (300mg/kg) into abdominal cavity for anesthesia according to 3mL/kg dosage, collecting blood from abdominal aorta, centrifuging at 3000rpm for 10min, and collecting supernatant for detecting oxidative stress index and inflammatory factor index.

5.2 taking materials and examining pathology

After the blood collection, the rats were sacrificed by cutting off abdominal aorta acute blood loss, the liver, spleen and thymus were rapidly separated, weighed, and organ coefficients (g/100g) were calculated. Organ coefficient 100 × (organ weight/body weight).

The liver tissue was observed for changes in color, texture, size, etc., recorded and photographed. Separating the left upper leaf of liver, fixing in 10% formalin solution, replacing fresh 10% formalin solution for 1 time after 6h, fixing for 24h, preparing pathological tablet, staining with hematoxylin-eosin (HE), and observing histopathological change under light microscope.

Separating left lower lobe of liver, collecting liver tissue of about 300mg from the same part, adding ice physiological saline according to liver weight: volume ratio of 1: 9, cutting, homogenizing with homogenizer to obtain 10% liver tissue homogenate, centrifuging at 3000rpm for 10min, and collecting supernatant. The protein level (ug/mL) of the liver tissue homogenate supernatant is detected by using a BCA method, and the specific operation steps are carried out according to the kit instructions.

5.3 liver function indices serum ALT, AST, GGT, ALB, TP and TBIL levels were measured using a fully automatic biochemical analyzer.

5.4 indices of cytokines serum samples or supernatants from liver homogenates were taken and tested for IL-1 β, IL-6, IL-10, TNF- α and TGF- β levels using ELISA.

5.5 oxidative stress index serum samples or liver tissue homogenate supernatants were taken and tested for SOD, MDA, GSH-PX, CAT and NO levels.

6. Data statistics SPSS17.0 statistics were used to obtainThe form shows that when P is less than 0.05, the data difference is considered to have statistical significance; measuring data according to normal distributionThe variance homogeneity is determined by adopting single-factor variance Test analysis and using LSD Test to carry out pairwise comparison analysis; if the data is not normal or the variance is not uniform, a Kruskal-Wallis H Test analysis is adopted, and a Mann-Whitney U Test is used for pairwise comparison analysis.

Example 2 pharmacodynamic test of Huocheng mother capsule for resisting acute liver injury

1. Body weight

Compared with the normal group, the weight gain and the total weight gain of the rats in the model group are not obviously different (P is more than 0.05); compared with the model group, the difference of the weight and the weight increase of each dose group of the Chinese knotweed herb capsules during the test period has no significance (P is more than 0.05); d₆The weight gains of silymarin group and Chinese knotweed herb dose groups are obviously increased (P is less than 0.05 or P is less than 0.01), and the differences of the weight gains and the total weight gains at other time points are not obvious (P is more than 0.05), so the Chinese knotweed herb capsules are considered to have no obvious influence on the weight of rats and are detailed in tables 1 to 4.

TABLE 1 Chinese charcoal master capsule Pair CCl₄Effect of weight on rats with acute liver injury

Table.1 Effects ofPolygonum chinense L.capsuleson body weight of acute liver injury rats induced by CCl₄

Note: in comparison with the set of models,^▲P＜0.05，^▲▲p is less than 0.01, and the number n of D3 and D6 animals in the low dose group is 9.

TABLE 2 Chinese charcoal female capsule Pair CCl₄Effect of weight on rats with acute liver injury

Table.2 Effects ofPolygonum chinense L.capsuleson body weight of acute liver injury rats induced by CCl₄

Note: in comparison with the set of models,^▲P＜0.05，^▲▲P＜0.01。

TABLE 3 Chinese medicine capsule pair CCl₄Effect of weight gain in rats with acute liver injury

Table.3 Effects ofPolygonum chinense L.capsuleson body weight gain of acute liver injury rats induced by CCl₄

Note: in comparison with the set of models,^▲P＜0.05，^▲▲P＜0.01。

TABLE 4 Chinese medicine capsule pair CCl₄Effect of weight gain in rats with acute liver injury

Table.4 Effects ofPolygonum chinense L.capsuleson body weight gain of acute liver injury rats induced by CCl₄

Note: in comparison with the set of models,^▲P＜0.05，^▲▲P＜0.01。

2. organ weight and organ coefficient

The weight and coefficient difference of spleen and thymus among all groups are not significant (P is more than 0.05); compared with the normal group, the liver weight and the coefficient of the rats in the model group are both obviously increased (P is less than 0.01); compared with the model group, the liver weight and the coefficient of the silymarin group rats are reduced, wherein the difference of the liver coefficients is significant (P is less than 0.05); the liver weight and the coefficient of rats in each dose group of the Chinese knotweed herb are obviously reduced (P is less than 0.05 or P is less than 0.01), so CCl is considered₄The weight and coefficient of spleen and thymus of the rat are not obviously affected, but the weight and coefficient of liver of the rat are increased, and the fire charcoal mother capsule has obvious improvement effect on the weight and coefficient of liver of the rat, which is detailed in tables 5 and 6.

TABLE 5 Chinese medicine capsule pair CCl₄Effect of visceral weight in rats with acute liver injury

Table.5 Effects ofPolygonum chinense L.capsuleson organ weight of acute liver injury rats induced by CCl₄

Note: in comparison with the set of models,^▲P＜0.05，^▲▲P＜0.01。

TABLE 6 Chinese medicine capsule pair CCl₄Influence of organ coefficient of rat with acute liver injury

Table.6 Effects ofPolygonum chinense L.capsuleson organ indexes of acute liver injury rats induced by CCl₄

Note: in comparison with the set of models,^▲P＜0.05，^▲▲P＜0.01。

3. liver function index

Compared with the normal group, the rat liver function indexes ALT, AST, TBIL, GLOB and GGT levels of the model group are all obviously increased (P < 0.05 or P < 0.01), and ALB, TP and A/G levels are all obviously reduced (P < 0.01); the silymarin group rats all had reduced levels of ALT, AST, TBIL, GLOB and GGT, with significant reductions in ALT, AST and GGT levels (P < 0.05 or P < 0.01) and significant increases in ALB, TP and A/G levels (P < 0.05 or P < 0.01), as compared to the model group; the rat liver function indexes ALT, AST, TBIL, GLOB and GGT levels of each dose group of the leontopodium chinense are all reduced, while ALB, TP and A/G levels are all increased. Wherein the low and medium dose groups AST and GLOB, the ALT, AST, TBIL, GLOB and GGT levels of the medium and high doses are obviously reduced (P < 0.05 or P < 0.01), and the ALB, A/G and TP levels of the medium and high doses are obviously increased (P < 0.05 or P < 0.01), so CCl is considered to be₄Can cause the abnormal change of the liver function index of the rat, and the Huocheng mother capsule has obvious improvement effect on the liver function index of the rat, and the details are shown in tables 7 and 8.

TABLE 7 Chinese medicine capsule pairs CCl₄Influence of liver function index of rat with acute liver injury

Table.7 Effects ofPolygonum chinense L.capsuleson liver function indexes of acute liver injury rats induced by CCl₄

Note: in comparison with the set of models,^▲P＜0.05，^▲▲p < 0.01, and n is 10 except for the low dose group of n 9.

TABLE 8 Chinese medicine capsule pairs CCl₄Influence of liver function index of rat with acute liver injury

Table.8 Effects ofPolygonum chinense L.capsuleson liver function indexes of acute liver injury rats induced by CCl₄

Note: in comparison with the set of models,^▲P＜0.05，^▲▲p < 0.01, and n is 10 except for the low dose group of n 9.

4. Oxidative stress index

Compared with the normal group, the levels of SOD, CAT and GSH in the serum, the liver tissue and the MDA in the serum and the liver tissue of the model group of rats are all obviously reduced (P is less than 0.01), and the levels of NO in the serum, the liver tissue and the serum are all obviously increased (P is less than 0.05 or P is less than 0.01); compared with the model group, the levels of SOD, CAT and GSH in serum, liver tissues and MDA and serum NO in the rat in the silymarin group are all obviously increased (P is less than 0.05 or P is less than 0.01), and the levels of MDA and serum NO in the rat in the serum, the liver tissues and the serum are all obviously reduced (P is less than 0.05 or P is less than 0.01); the rat serum, liver tissue SOD and liver tissue CAT levels of each dose group of the Chinese knotweed herb are all increased, and the serum, liver tissue MDA and serum NO levels are all reduced. Wherein the low-dose group liver tissues CAT and GSH, the medium-and high-dose group serum, liver tissue SOD, CAT and high-dose group liver tissues GSH are obviously increased (P is less than 0.05 or P is less than 0.01), the low-and high-dose group serum MDA, medium-and high-dose group serum, liver tissue MDA and serum NO level are obviously reduced (P is less than 0.05 or P is less than 0.01)0.01), CCl is considered₄Can cause the oxidative stress index of rats to be abnormally changed, and the fire charcoal female capsule has obvious improvement effect on the oxidative stress index of the serum and the liver tissues of the rats, which is detailed in tables 9 and 10.

TABLE 9 Chinese charcoal female Capsule Pair CCl₄Influence of rat serum SOD, MDA and NO on acute liver injury

Table.9 Effects ofPolygonum chinense L.capsuleson serum SOD，MDA and NO of acute liver injury rats induced by CCl₄

Note: in comparison with the set of models,^▲P＜0.05，^▲▲p < 0.01, and n is 10 except for the low dose group of n 9.

TABLE 10 Chinese charcoal female capsule Pair CCl₄Influence of SOD, MDA, CAT and GSH on liver tissue of rat with acute liver injury

Table.10 Effects ofPolygonum chinense L.capsuleson liver SOD，MDA，CAT and GSH of acute liver injury rats induced by CCl₄

Note: in comparison with the set of models,^▲P＜0.05，^▲▲p < 0.01, and n is 10 except for the low dose group of n 9.

5. Index of inflammatory factor

Compared with the normal group, the levels of IL-1 beta, IL-6 and TNF-alpha in the serum, liver tissues and the model group of rats are obviously increased (P is less than 0.01), and the level of IL-10 in the serum is obviously reduced (P is less than 0.01); compared with the model group, the silymarin group rat model group has the advantages that the levels of the serum, the hepatic tissue IL-1 beta, the IL-6 and the TNF-alpha of the rat are all obviously reduced (P is less than 0.05 or P is less than 0.01), and the level of the serum IL-10 is obviously increased (P is less than 0.05); the serum, liver tissue IL-1 beta, IL-6 and TNF-alpha levels of rats in each dose group of the Chinese knotweed are all reduced, wherein the serum IL-6, liver tissue TNF-alpha levels of rats in the low dose group and the serum, liver tissue IL-1 beta, IL-6 and TNF-alpha levels of rats in the medium and high dose groups are significantly reduced (P is less than 0.05 or P is less than 0.01), and the serum IL-10 levels of rats in each dose group are significantly increased (P is less than 0.05 or P is less than 0.01), so CCl is considered to be₄Can cause the abnormal change of the inflammatory factor index of the rat, and the fire charcoal female capsule has obvious improvement effect on the inflammatory factor index of the serum and the liver tissue of the rat, and is detailed in tables 11 and 12.

TABLE 11 Chinese charcoal female capsule Pair CCl₄Influence of serum IL-1 beta, IL-6, IL-10 and TNF-alpha on acute liver injury in rats

Table.11 Effects ofPolygonum chinense L.capsuleson serum IL-1β，IL-6，IL-10 and TNF-α of acute liver injury rats induced by CCl₄

Note: in comparison with the set of models,^▲P＜0.05，^▲▲p < 0.01, and n is 10 except for the low dose group of n 9.

TABLE 12 Chinese medicine capsule pairs CCl₄Influence of IL-1 beta, IL-6 and TNF-alpha on liver tissue of rat with acute liver injury

Table.12 Effects ofPolygonum chinense L.capsuleson liver IL-1β、IL-6 and TNF-α of acute liver injury rats induced by CCl₄

Note: in comparison with the set of models,^▲P＜0.05，^▲▲P＜0.01。

6. pathological examination

6.1 gross necropsy visual observation

The liver of the normal group of rats is dark red, uniform, free of mottle, smooth and glossy; the liver of the rat in the model group is obviously swollen, the color is yellow brown, and the liver tissues can be seen to be uniformly distributed with yellow-white particles. Compared with the model group, the liver swelling degree of the silymarin group rat is obviously reduced, the liver tissue does not have yellow and white particles, and the pathological change of the liver tissue is obviously improved; the liver swelling degree of rats in the low-dose group of the Huochou capsules is obviously reduced, yellow and white particles of liver tissues are obviously reduced, the liver swelling degree of rats in the medium-dose group is obviously reduced, the liver tissues do not see the yellow and white particles, but the liver color is slightly yellow and light, the liver swelling degree of rats in the high-dose group is obviously reduced, the liver tissues do not see the yellow and white particles, and the liver color is not obviously different from that of the normal group. Therefore, it is considered that CCl₄Can cause pathological change of liver tissue of rats, the liver tissue is light and yellow in color and luster which can be observed by naked eyes, the liver tissue can be uniformly distributed in yellow-white granules, and the Huocheng capsules can improve CCl to different degrees₄The resulting pathological changes of the liver, of which high doses are optimal.

6.2 Observation under the pathological microscope

The normal group of rats has large, medium and round hepatocyte nuclei, clear cell outline, radial arrangement, regular arrangement and no degeneration and necrosis of hepatocytes; the rat liver cells of the model group have large-area pathological changes, obvious deformation edema, a large amount of nucleus fixation and shrinkage, small nucleus, irregular shape, disordered cell arrangement, unclear outline and large amount of liver cell degeneration necrosis appear; compared with the model group, the pathological damage of the liver cells of the rat in the silymarin group is obviously improved, the form of edema is obviously reduced, the nuclear consolidation of cell nuclei is obviously reduced, the cell arrangement is more orderly, and the number of the degenerated necrotic cells is obviously reduced; the lesion area around the liver cell blood vessel of rats in each dose group of the Huocheng Chinese medicinal capsule is obviously reduced, the number of lesion cells is obviously reduced, the nuclear consolidation of cell nucleus is obviously reduced, and the pathological damage of liver cells is obviously improved, wherein the change is most obvious in high dose group.