阅读说明：本技术 一种生物离子钙饲料添加剂的制备方法 (Preparation method of biological ionized calcium feed additive ) 是由 潘东冰 张勇 覃鸿妮 周婉婉 于 2020-08-31 设计创作，主要内容包括：本发明涉及一种生物离子钙饲料添加剂的制备方法,首先将枯草芽孢杆菌及发酵酵母的菌株接种培养得到发酵浓缩液,然后分别将发酵浓缩液与饲料原料及离子钙溶液进行混合搅拌并烘干得到发酵固体物,最终将两种发酵固体物与包被乳酸菌粉、天然麦饭石粉混合得到成品,用于添加至饲料中饲养畜禽,能够提高畜禽成活率及免疫力,同时能够降低饲料的消费量,具有广阔的市场使用前景,本发明的制备方法简单、生产周期短,且各原料投入成本相对较低,通过混合能够实现功效最大化,其中离子钙能够使畜禽的吸收达到最大,促进生长速度,改善产品品质,同时能够实现畜禽类粪便排泄物无臭化、无害化、资源化及无抗化。(The invention relates to a preparation method of biological ionized calcium feed additive, which comprises the steps of inoculating and culturing strains of bacillus subtilis and yeast to obtain fermentation concentrated solution, mixing and stirring the fermentation concentrated solution, feed raw materials and ionized calcium solution respectively, drying to obtain fermentation solid, mixing the two fermentation solid, coated lactic acid bacteria powder and natural medical stone powder to obtain a finished product, adding the finished product into feed to raise livestock and poultry, improving the survival rate and immunity of the livestock and poultry, reducing the consumption of the feed, and having wide market application prospect Harmless, resource and non-resistance.)

1. A preparation method of biological ionized calcium feed additive is characterized in that: the preparation method comprises the following specific steps:

(1) selection of strains and culture medium:

strain a: bacillus subtilis with the preservation number of CGMCC No.18614, wherein the weight percentage of the culture medium is as follows: 0.5-0.8% of peptone, 0.25-0.5% of yeast extract powder, 0.25-0.5% of sodium chloride, 0.5-0.8% of glucose and the balance of distilled water, wherein the pH value is 7.2, and then the fermentation concentrate A is obtained by culturing at the constant temperature of 30-35 ℃ for 48-55 h;

and (3) strain B: the fermentation yeast Saccharomyces fermentatii with the preservation number of CGMCC No.18616 comprises the following culture media in percentage by weight: 0.5-0.8% of peptone, 0.25-0.5% of yeast extract powder, 0.25-0.5% of sodium chloride, 0.5-0.8% of glucose and the balance of distilled water, wherein the pH value is 7.2, and then the fermentation concentrate B is obtained by culturing at the constant temperature of 30-35 ℃ for 48-55 h;

(2) preparation of ionized calcium solution:

ionized calcium solution A: mixing and stirring the water element water and the ionized calcium powder at the temperature of 30-35 ℃ for 3-6 min to obtain an ionized calcium solution A, wherein the water element water and the ionized calcium powder are mixed according to the weight ratio of 45-55: 1;

ionic calcium solution B: mixing and stirring the water element water and the ionized calcium powder at the temperature of 30-35 ℃ for 3-6 min to obtain an ionized calcium solution B, wherein the water element water and the ionized calcium powder are mixed according to the weight ratio of 90-100: 1;

(3) preparation of fermentation fixture:

fermentation solid A: mixing and stirring the fermentation concentrated solution A, the defatted rice bran, the wheat bran, the corn flour, the glucose and the ionized calcium solution A for 8-12 min according to the weight ratio of 2:1:10:5:1:2, and then fermenting at the temperature of 30-35 ℃ for 100-120 h in a heat preservation manner;

fermentation solid B: mixing and stirring the fermentation concentrated solution B, the defatted rice bran, the wheat bran, the corn flour, the glucose and the ionized calcium solution B according to the weight ratio of 2:6:5:4:1:2 for 8-12 min, and then fermenting at the temperature of 30-35 ℃ for 100-120 h in a heat preservation manner;

(4) drying the fermented solid:

after fermentation, the fermented solid A and the fermented solid B are spread and placed, dried by hot air for 24 hours until the mass water content is less than or equal to 10 percent, and crushed and sieved under the aseptic condition, wherein the mesh number is 100 meshes;

(5) preparing a finished product of the biological ion calcium feed additive:

mixing and stirring the dried fermented solid A and fermented solid B, the coated lactic acid bacteria powder and the natural medical stone powder according to the weight ratio of 200:200:2:1 for 8-12 min to obtain the biological ionized calcium feed additive, and packaging.

2. The method for preparing the biological ionized calcium feed additive according to claim 1, which is characterized in that: in the step (1), the number of the strains A of the Bacillus subtilis in the fermentation concentrated solution A is more than 10⁹The number of the strains B for fermenting the yeast Saccharomyces fermentati in the fermentation concentrated solution B is more than 10⁸/ml。

3. The method for preparing the biological ionized calcium feed additive according to claim 1, which is characterized in that: in the step (2), the concentration of the water containing the ion calcium solution A and the fermentation solid B added in the preparation process is 1-1.2 ppm.

4. The method for preparing the biological ionized calcium feed additive according to claim 1, which is characterized in that: in the step (3), the water contents of the defatted rice bran, wheat bran and corn flour added in the preparation process of the fermented solid A and the fermented solid B are respectively 8-12%.

5. The method for preparing the biological ionized calcium feed additive according to claim 1, which is characterized in that: in the step (4), the fermented solid A and the fermented solid B are firstly dried for 1-2 hours at the initial temperature of 35-40 ℃, and then continuously dried by hot air for 22-23 hours at the process temperature of 55-65 ℃.

6. The method for preparing the biological ionized calcium feed additive according to claim 1, which is characterized in that: in the step (5), the mesh number of the coating lactic acid bacteria powder is 1000 meshes, and the mesh number of the natural medical stone powder is 3000 meshes.

Technical Field

The invention relates to the technical field of feed preparation methods, in particular to a preparation method of a biological ionized calcium feed additive.

Background

In addition to feeding normal feed in the animal feeding process, some advanced feed additives are developed and applied in recent years, the feed additives refer to a small amount or trace substances added in the feed production processing and using processes, and the dosage of the feed additives in the feed is small but the effect is obvious; the feed additive is a raw material inevitably used in modern feed industry, and has obvious effects on strengthening the nutritive value of basic feed, improving the production performance of animals, ensuring the health of the animals, saving the feed cost, improving the quality of animal products and the like.

At present, two types of feed additives mainly appear on the market, one of which has good use effect but high input cost, the preparation of the animal feed additive is relatively complicated, the variety of the internal added raw materials is various, the input cost of the feed additive also comprises equipment used in the preparation, such as a traditional Chinese medicine microecological feed additive, and the feed additive contains a large amount of beneficial bacteria (active lactic acid bacteria, bifidobacteria and bacillus), complex enzyme, chelating peptide, mildew removing agent and the like, and the feed additive can be rapidly propagated after entering the livestock and poultry body as the feed, on one hand, metabolites of the bacteria are added to neutralize enterotoxin, and the growth of other harmful flora is inhibited, on the other hand, normal microbial flora is formed in the host, so that main vitamins are synthesized for the host, nutrition is provided, and the invasion of pathogenic bacteria is inhibited, the feed additive has remarkable effect, and the growth and the quality of the livestock and poultry, but the required investment cost is too high; secondly, the investment cost is low, and the additives can cause serious drug residue in livestock and poultry products and quality reduction, bring drug resistance and obesity to human and environment, influence the health of human, cause poor texture of cultured animals and the like; aiming at the problems, how to control the input cost while ensuring the quality of livestock and poultry products is the target to be realized at the present stage, the invention develops a preparation method of the biological ionized calcium feed additive to solve the problems in the prior art, and a technical scheme which is the same as or similar to the invention is not found after retrieval.

Disclosure of Invention

The invention aims to: the preparation method of the biological ion calcium feed additive is provided to solve the problems of complex preparation and various types of added raw materials, which result in high cost, aiming at the high-efficiency feed additive in the prior art; and meanwhile, the problems that the quality of livestock and poultry products is reduced, the human health and the environment are harmed and the like caused by unqualified feed products are further solved for the low-cost feed additive appearing in the market.

The technical scheme of the invention is as follows: a preparation method of a biological ion calcium feed additive comprises the following specific steps:

(1) selection of strains and culture medium:

strain a: the Bacillus subtilis with the preservation number of CGMCC No.18614 comprises the following culture media in percentage by weight: 0.5-0.8% of peptone, 0.25-0.5% of yeast extract powder, 0.25-0.5% of sodium chloride, 0.5-0.8% of glucose and the balance of distilled water, wherein the pH value is 7.2, and then the fermentation concentrate A is obtained by culturing at the constant temperature of 30-35 ℃ for 48-55 h;

and (3) strain B: the fermentation yeast Saccharomyces fermentatii with the preservation number of CGMCC No.18616 comprises the following culture media in percentage by weight: 0.5-0.8% of peptone, 0.25-0.5% of yeast extract powder, 0.25-0.5% of sodium chloride, 0.5-0.8% of glucose and the balance of distilled water, wherein the pH value is 7.2, and then the fermentation concentrate B is obtained by culturing at the constant temperature of 30-35 ℃ for 48-55 h;

(2) preparation of ionized calcium solution:

ionized calcium solution A: mixing and stirring the water element water and the ionized calcium powder at the temperature of 30-35 ℃ for 3-6 min to obtain an ionized calcium solution A, wherein the water element water and the ionized calcium powder are mixed according to the weight ratio of 45-55: 1;

ionic calcium solution B: mixing and stirring the water element water and the ionized calcium powder at the temperature of 30-35 ℃ for 3-6 min to obtain an ionized calcium solution B, wherein the water element water and the ionized calcium powder are mixed according to the weight ratio of 90-100: 1;

(3) preparation of fermentation fixture:

fermentation solid A: mixing and stirring the fermentation concentrated solution A, the defatted rice bran, the wheat bran, the corn flour, the glucose and the ionized calcium solution A for 8-12 min according to the weight ratio of 2:1:10:5:1:2, and then fermenting at the temperature of 30-35 ℃ for 100-120 h in a heat preservation manner;

fermentation solid B: mixing and stirring the fermentation concentrated solution B, the defatted rice bran, the wheat bran, the corn flour, the glucose and the ionized calcium solution B according to the weight ratio of 2:6:5:4:1:2 for 8-12 min, and then fermenting at the temperature of 30-35 ℃ for 100-120 h in a heat preservation manner;

(4) drying the fermented solid:

after fermentation, the fermented solid A and the fermented solid B are spread and placed, dried by hot air for 24 hours until the mass water content is less than or equal to 10 percent, and crushed and sieved under the aseptic condition, wherein the mesh number is 100 meshes;

(5) preparing a finished product of the biological ion calcium feed additive:

mixing and stirring the dried fermented solid A and fermented solid B, the coated lactic acid bacteria powder and the natural medical stone powder according to the weight ratio of 200:200:2:1 for 8-12 min to obtain the biological ionized calcium feed additive, and packaging.

Preferably, in the step (1), the concentrated solution A is fermented with the hayThe number of the strain A of the Bacillus subtilis is more than 10⁹The number of the strains B for fermenting the yeast Saccharomyces fermentati in the fermentation concentrated solution B is more than 10⁸/ml。

Preferably, in the step (2), the concentration of the aqueous solution of the ionized calcium A and the aqueous solution of the fermented solid B added in the preparation process is 1 to 1.2 ppm.

Preferably, in the step (3), the water content of the defatted rice bran, wheat bran and corn flour added in the preparation process of the fermented solid A and the fermented solid B is 8-12% respectively.

Preferably, in the step (4), the fermented solid A and the fermented solid B are firstly dried at an initial temperature of 35-40 ℃ for 1-2 hours, and then continuously dried by hot air at a process temperature of 55-65 ℃ for 22-23 hours.

Preferably, in the step (5), the mesh number of the coated lactic acid bacteria powder is 1000 meshes, and the mesh number of the natural medical stone powder is 3000 meshes.

Compared with the prior art, the invention has the advantages that:

(1) according to the invention, the fermentation concentrated solution containing the bacillus subtilis and the fermentation yeast is respectively mixed with the feed raw materials, stirred and dried, and the ionized calcium is added in the mixing process to prepare the feed additive for feeding livestock and poultry, so that the survival rate and the immunity of the livestock and poultry can be improved, the consumption of the feed can be reduced, and the feed additive has a wide market application prospect.

(2) The preparation method is simple, the production period is short, the input cost of each raw material is relatively low, the effect maximization can be realized through mixing, the ionized calcium can maximize the absorption of livestock and poultry, the growth speed is promoted, the product quality is improved, and meanwhile, the deodorization, the harmlessness, the recycling and the antibiotic-free of livestock and poultry excrement can be realized.

Drawings

The invention is further described with reference to the following figures and examples:

FIG. 1 is a graph showing the comparison of the delivery rate of poultry-chickens tested in the method for preparing a bio-ionic calcium feed additive according to the present invention (with fermentation concentrate A and fermentation concentrate B as variables);

FIG. 2 is a graph showing the comparison of the feed consumption of poultry-chickens tested in the method for preparing a bio-ionic calcium feed additive according to the present invention (with fermentation concentrate A and fermentation concentrate B as variables);

FIG. 3 is a graph showing the comparison of the delivery rate of poultry-chickens tested in the method for preparing a bio-ionized calcium feed additive according to the present invention (ionized calcium solution A and ionized calcium solution B are used as variables);

fig. 4 is a graph showing the comparison of the feed consumption of poultry-chickens tested in the preparation method of the bio-ionized calcium feed additive of the invention (with ionized calcium solution a and ionized calcium solution B as variables).

Detailed Description

The present invention will be further described in detail with reference to the following specific examples: