阅读说明：本技术 一种富含葛花异黄酮的葛花提取物及其制备方法和应用 (Kudzuvine flower extract rich in pueraria flower isoflavone and preparation method and application thereof ) 是由 谷素静 李林正 金子恒 文雁君 于 2020-06-22 设计创作，主要内容包括：本发明公开了一种富含葛花异黄酮的葛花提取物及其制备方法和应用,该方法将葛花用低浓度乙醇搅拌提取,提取液过滤分离,真空浓缩回收至一定固形物含量的浓缩液,再加入高浓度乙醇静置,沉淀去杂质,放置分层,离心分离,取上清液减压浓缩,直接喷雾干燥呈粉末,既得葛花提取物。采用本发明制备得到的葛花提取物异黄酮含量高达30％以上,总异黄酮回收率大于90％,同时产品水溶性良好,与现存市场上葛花提取物相比,葛花提取物加水可得到均匀高度澄清的液体,适合于饮料行业应用,扩大了适用范围。本发明方法设备要求低,操作简单可行,生产成本低,适用于工业化生产,有较大的应用价值。(The invention discloses a kudzuvine flower extract rich in pueraria flower isoflavone and a preparation method and application thereof. The content of isoflavone in the pueraria flower extract prepared by the method is up to more than 30%, the recovery rate of the total isoflavone is more than 90%, and meanwhile, the water solubility of the product is good. The method has the advantages of low equipment requirement, simple and feasible operation, low production cost, suitability for industrial production and higher application value.)

1. A flos Puerariae Lobatae extract rich in flos Puerariae Lobatae isoflavone is characterized in that flavone content in the extract is above 30%.

2. The method for preparing a pueraria lobata extract rich in pueraria lobata isoflavones according to claim 1, comprising the steps of:

adding 20-50% ethanol solution into flos Puerariae Lobatae for extraction, wherein the weight of ethanol solution is 3-12 times of the total weight of flos Puerariae Lobatae raw materials, the extraction temperature is 20-80 deg.C, and stirring for extraction for 1-4 hr; extracting for 1-3 times, and mixing extractive solutions; filtering the extracting solution; concentrating the filtrate under reduced pressure to obtain extract; adding anhydrous ethanol into the extract until the ethanol concentration is 70-95%, standing at room temperature or refrigerated storage temperature of 30-20 deg.C, and standing for 1-4 hr; centrifuging the obtained ethanol solution to obtain supernatant; centrifuging to obtain supernatant, concentrating under reduced pressure to obtain extract, and drying to obtain flos Puerariae Lobatae extract.

3. The method for preparing a pueraria lobata extract rich in pueraria lobata isoflavones according to claim 2, comprising the steps of:

1) preparing raw materials: taking dried flos Puerariae Lobatae, and making into coarse powder;

2) extraction: taking the coarse powder raw materials, adding an ethanol solution with the weight 5-10 times of the total weight of the raw materials, wherein the concentration of the ethanol is 30-40%, the extraction temperature is 60-80 ℃, stirring and extracting for 1-2h, extracting for 2-3 times, and combining the extracting solutions;

3) and (3) filtering: performing plate-and-frame filtration on the extracting solution, and separating material residues;

4) and (3) concentrating under reduced pressure: concentrating the filtrate under reduced pressure to obtain extract with solid content of 10-40%;

5) standing high-concentration ethanol: adding anhydrous ethanol into the extract until the ethanol concentration is 80-90%, standing at room temperature or cold storage temperature of 25-20 deg.C, and standing for 1-4 hr;

6) centrifuging: centrifuging the ethanol solution of 5) to obtain a supernatant, wherein the centrifugation speed is 2000-;

7) and (3) drying: concentrating the supernatant under reduced pressure to obtain extract, and spray drying to obtain flos Puerariae Lobatae extract.

4. The method for preparing a pueraria lobata extract rich in pueraria lobata isoflavones according to claim 2, comprising the steps of:

1) preparing raw materials: taking dried flos Puerariae Lobatae, making into coarse powder, and sieving with 40 mesh sieve;

2) extraction: taking the coarse powder, adding an ethanol solution with the weight 6 times of the total weight of the raw materials, wherein the ethanol concentration is 40%, the extraction temperature is 60 ℃, stirring and extracting for 1h for 2 times, and combining the extracting solutions;

3) filtering, and performing plate-and-frame filtration on the extract to separate material residues;

4) and (3) concentrating under reduced pressure: concentrating the filtrate under reduced pressure to obtain extract with solid content of 25%,

5) standing high-concentration ethanol: adding anhydrous ethanol into the extract until the ethanol concentration is 80%, standing at room temperature or refrigerated storage temperature to 20 deg.C, and standing for 1-4 hr;

6) centrifuging: centrifuging the ethanol solution of 5) to obtain a supernatant, wherein the centrifugation speed is 3000 r/min;

7) and (3) drying: concentrating the supernatant under reduced pressure to obtain extract, and spray drying to obtain flos Puerariae Lobatae extract.

5. Use of the pueraria flower extract rich in pueraria flower isoflavone according to claim 1 in foods, health products and medicines.

6. Use of the pueraria flower extract rich in pueraria flower isoflavone according to claim 1 in a beverage.

7. The use of the pueraria flower extract rich in pueraria flower isoflavones according to claim 1 in an oral liquid.

8. Use of the flos puerariae lobatae extract prepared by the preparation method of the flos puerariae lobatae extract according to any one of claims 2 to 4 in foods, health products and medicines.

9. The use of a pueraria flower extract prepared by the method according to any one of claims 2 to 4 in a beverage.

10. The use of a pueraria flower extract prepared by the method according to any one of claims 2 to 4 in an oral liquid.

Technical Field

The invention relates to medicinal and edible raw materials, in particular to an extraction and preparation method of effective components, and particularly relates to a pueraria flower extract rich in pueraria flower isoflavone, and a preparation method and application thereof.

Background

Pueraria flower is dried flower of Pueraria lobata (Willd.) Ohwi of Leguminosae. Is collected and carried by the Chinese medicinal material of the ministry of health of the people's republic of China, has no toxicity, has sweet and cool medicinal properties and flavor, enters the stomach channel, and has the effects of dispelling the effects of alcohol and activating the spleen. Can be used for treating excessive drinking, headache, dizziness, polydipsia, chest and diaphragm distention, acid regurgitation, etc. and stomach qi injury. In addition, modern researches find that the pueraria flower also has the effects of reducing blood sugar, reducing blood fat, resisting oxidation and allergy, improving memory, reducing body fat, losing weight and the like. The flos Puerariae Lobatae contains flavonoids, saponins and volatile oil compounds, wherein the most studied active ingredient is flavonoids, including tectorigenin, tectoridin, pueraria glycoside, irisolidone, etc.

At present, the pueraria flower extraction process mainly adopts water extraction and organic solvent extraction to obtain an extract with or without refining, but the extract obtained by the methods has low isoflavone content, complex process and low yield, or has poor dissolving state in water, so that the requirements of beverage and oral liquid food industry are difficult to meet. CN 101239092A pueraria flower isoflavone extract, its extraction method, pharmaceutical composition and its application in pharmacy, although the solubility is improved, it needs to be extracted by adding organic solvent after alumina treatment, the operation steps are tedious, the extraction rate is low, the cost is high. Patent publication No. CN 101129447A relates to a method for extracting total isoflavone from flos Puerariae Lobatae and its application. The method adopts ethanol extract to dilute with 10-60% ethanol, and adopts macroporous adsorbent resin purification method to prepare high-content isoflavone extract, and the process has high energy consumption and high cost.

Disclosure of Invention

In order to overcome the defects of the background art, the invention aims to solve the application problem of the solubility of the pueraria flower extract in food and the problem of quality improvement, and provides the pueraria flower isoflavone-rich extract with good water solubility and the preparation method with simple operation and low cost.

The pueraria flower extract rich in pueraria flower isoflavone provided by the invention has good water solubility, and the isoflavone content in the extract is more than 30%.

The invention also provides a preparation method of the pueraria flower extract rich in pueraria flower isoflavone, which comprises the following steps:

adding 20-50% ethanol solution into flos Puerariae Lobatae for extraction, wherein the weight of ethanol solution is 3-12 times of the total weight of flos Puerariae Lobatae raw materials, the extraction temperature is 20-80 deg.C, and stirring for extraction for 1-4 hr; extracting for 1-3 times, and mixing extractive solutions; filtering the extracting solution; concentrating the filtrate under reduced pressure to obtain extract; adding anhydrous ethanol into the extract until the ethanol concentration is 70-95%, standing at room temperature or refrigerated storage temperature of 30-20 deg.C, and standing for 1-4 hr; centrifuging the obtained ethanol solution to obtain supernatant; centrifuging to obtain supernatant, concentrating under reduced pressure to obtain extract, and drying to obtain flos Puerariae Lobatae extract.

More specifically, the preparation method comprises the following steps:

1) preparing raw materials: taking dried flos Puerariae Lobatae, and making into coarse powder;

2) extraction: taking the coarse powder raw materials, adding an ethanol solution with the weight 5-10 times of the total weight of the raw materials, wherein the concentration of the ethanol is 30-40%, the extraction temperature is 60-80 ℃, stirring and extracting for 1-2h, extracting for 2-3 times, and combining the extracting solutions;

3) and (3) filtering: performing plate-and-frame filtration on the extracting solution, and separating material residues;

4) and (3) concentrating under reduced pressure: concentrating the filtrate under reduced pressure to obtain extract with solid content of 10-40%;

5) standing high-concentration ethanol: adding anhydrous ethanol into the extract until the ethanol concentration is 80-90%, standing at room temperature or cold storage temperature of 25-20 deg.C, and standing for 1-4 hr;

6) centrifuging: centrifuging the ethanol solution of 5) to obtain a supernatant, wherein the centrifugation speed is 2000-;

7) and (3) drying: concentrating the supernatant under reduced pressure to obtain extract, and spray drying to obtain flos Puerariae Lobatae extract.

More preferably, the preparation method of the pueraria flower extract rich in pueraria flower isoflavone provided by the invention comprises the following steps:

1) preparing raw materials: taking dried flos Puerariae Lobatae, making into coarse powder, and sieving with 40 mesh sieve;

2) extraction: taking the coarse powder, adding an ethanol solution with the weight 6 times of the total weight of the raw materials, wherein the ethanol concentration is 40%, the extraction temperature is 60 ℃, stirring and extracting for 1h for 2 times, and combining the extracting solutions;

3) filtering, and performing plate-and-frame filtration on the extract to separate material residues;

4) and (3) concentrating under reduced pressure: concentrating the filtrate under reduced pressure to obtain extract with solid content of 25%,

5) standing high-concentration ethanol: adding anhydrous ethanol into the extract until the ethanol concentration is 80%, standing at room temperature or refrigerated storage temperature to 20 deg.C, and standing for 1-4 hr;

6) centrifuging: centrifuging the ethanol solution of 5) to obtain a supernatant, wherein the centrifugation speed is 3000 r/min;

7) and (3) drying: concentrating the supernatant under reduced pressure to obtain extract, and spray drying to obtain flos Puerariae Lobatae extract.

The invention provides application of a pueraria flower extract rich in pueraria flower isoflavone in food, health-care products and medicines, in particular application in beverages and oral liquids.

Compared with the prior art, the pueraria flower extract obtained by the invention has excellent solubility in water, high content of pueraria flower isoflavone and high yield, and can be used for food, beverage and oral liquid. The process operation is simple and feasible, and is suitable for industrial production.

Detailed Description